Rw5G018820

salt tolerance-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0015021
Physical Location & Seq
Forward (+)
24272278 .. 24274843
2566 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G018820.1

Sequence Viewer

Length: 855 bp
ATGAAGATACAGTGTAACGTGTGCGAGACGGCGGAGGCGAACGTGCTCTGCTGCGCCGACGAGGCGGCGCTCTGCTGGGCCTGCGACGAGAAGGTCCACGCCGCCAACAAGCTCGCCAGCAAGCACCAGAGGGTTCCTCTCTCCGCCTCCCACATGCCCAAGTGCGATATCTGCCAGGAGGCGGTTGGGTATTTTTTCTGTTTGGAGGATAGAGCTCTGCTGTGTAGGAACTGTGATGTTGCTGTACATACTGCGAATAGTTTTGTTTCCGGTCACCGGAGGTTTTTGCTGACCGGAATTAAAGTTGGGCCGGAGTCCAGTCCACCTGGGTCTGGTGGTGGTGGTGGTGGTGTTGCTGGGTCGTCTTCTTCTGTCAAACCGGACTCTGGGGCTGCGTCGAAATGTGAAGCAAACATTCAATTGGCGGAGGAATGCAAAGTGGCGCCGTCAAGTGTTGCTGGGATGTCTTTTGCTGGTGGTTCGGCAGCTGGGTCTGGTCCACAGTGGCCTATAGATGAGTTTCTGGGATTCACTGATTTCGATCAGAGCTTTGGGTACATGGATAATGGATCGTCTAAGGCTGACTGCGGTAAACTTGGGGCTTCTGATTCATCAATTTTAAGATCATCTGAGGAGCAAGAGGAATACGAGTGCATAGGTCAGGTGCCAGAGATCTCTTGGATGGTGCCAGAGGTACCTTCCCCGCCTACATCCTCGGGGCTATACTGGCCTAAAACTTATCAGAACCCATCGGATTCTGCAGTGTTTGTTCCAGACGTTTGCTATTCAGAAATGGAAAATCCTCTTCACTGTCAACAGAATGGTACTGTCTCAAAACGTCGTAGGCAATTCTAA

Protein Analysis

284

Amino Acids

30.16

Weight (kDa)

4.94

Isoelectric Point (pI)

61.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 52 - 93 2.1e-07 B-box zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015021)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10240
fragaria_vesca FvH4_3g17750
malus_domestica MD03G1247700.v1.1 MD11G1269800.v1.1
prunus_persica Prupe.4G156600_v2.0.a1
pyrus_communis pycom03g19640 pycom11g23830
rosa_chinensis RchiOBHm_Chr5g0029541
rosa_laevigata RLG00000033177
rosa_multiflora Rmu_sc0000210.1_g000014
rosa_roxburghii Rroxscaffold_1G00050400
rosa_samantha Rh5AG206900 Rh5BG205600 Rh5CG227700 Rh5DG209300
rosa_wichuraiana Rw5G018820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
Acc65I GGTACC 1 cut(s) 694
AccB1I GGYRCC 4 cut(s) 442, 664, 685, 694
AciI CCGC 8 cut(s) 32, 65, 102, 144, 182, 425, 588, 704
AclWI GGATC 1 cut(s) 577
AcyI GRCGYC 1 cut(s) 443
AfaI GTAC 4 cut(s) 246, 557, 696, 826
AfiI CCNNNNNNNGG 4 cut(s) 181, 276, 332, 386
AflIII ACRYGT 1 cut(s) 18
AgsI TTSAA 1 cut(s) 419
AjnI CCWGG 2 cut(s) 174, 325
AluBI AGCT 4 cut(s) 112, 215, 488, 549
AluI AGCT 4 cut(s) 112, 215, 488, 549
Alw21I GWGCWC 2 cut(s) 48, 217
Alw26I GTCTC 2 cut(s) 20, 835
AlwI GGATC 1 cut(s) 577
Ama87I CYCGRG 1 cut(s) 715
AoxI GGCC 4 cut(s) 78, 308, 506, 728
ApeKI GCWGC 3 cut(s) 51, 392, 485
Asp718I GGTACC 1 cut(s) 694
AspLEI GCGC 3 cut(s) 56, 70, 445
AspS9I GGNCC 4 cut(s) 78, 94, 308, 497
AsuHPI GGTGA 1 cut(s) 266
AvaI CYCGRG 1 cut(s) 715
AvaII GGWCC 2 cut(s) 94, 497
BanI GGYRCC 4 cut(s) 442, 664, 685, 694
BanII GRGCYC 1 cut(s) 217
BbsI GAAGAC 1 cut(s) 357
Bbv12I GWGCWC 2 cut(s) 48, 217
BbvI GCAGC 3 cut(s) 38, 379, 497
BccI CCATC 2 cut(s) 676, 757
BceAI ACGGC 2 cut(s) 45, 430
BciT130I CCWGG 2 cut(s) 176, 327
BcoDI GTCTC 2 cut(s) 20, 835
BfmI CTRYAG 2 cut(s) 510, 759
BfoI RGCGCY 2 cut(s) 71, 446
BglI GCCNNNNNGGC 1 cut(s) 62
BglII AGATCT 1 cut(s) 672
BisI GCNGC 5 cut(s) 52, 66, 102, 393, 486
BlsI GCNGC 5 cut(s) 53, 67, 103, 394, 487
Bme1390I CCNGG 2 cut(s) 176, 327
Bme18I GGWCC 2 cut(s) 94, 497
BmeT110I CYCGRG 1 cut(s) 715
BmgT120I GGNCC 4 cut(s) 78, 94, 308, 497
BmiI GGNNCC 5 cut(s) 135, 444, 666, 687, 696
BmrFI CCNGG 2 cut(s) 176, 327
BpiI GAAGAC 1 cut(s) 357
BplI GAGNNNNNCTC 2 cut(s) 121, 153
BsaBI GATNNNNATC 1 cut(s) 540
BsaHI GRCGYC 1 cut(s) 443
BsaJI CCNNGG 2 cut(s) 326, 714
BsaWI WCCGGW 4 cut(s) 269, 276, 293, 379
Bsc4I CCNNNNNNNGG 4 cut(s) 181, 276, 332, 386
Bse1I ACTGG 2 cut(s) 318, 731
Bse8I GATNNNNATC 1 cut(s) 540
BseBI CCWGG 2 cut(s) 176, 327
BseDI CCNNGG 2 cut(s) 326, 714
BseGI GGATG 3 cut(s) 468, 687, 710
BseJI GATNNNNATC 1 cut(s) 540
BseLI CCNNNNNNNGG 4 cut(s) 181, 276, 332, 386
BseMII CTCAG 1 cut(s) 621
BseNI ACTGG 2 cut(s) 318, 731
BseRI GAGGAG 1 cut(s) 647
BseXI GCAGC 3 cut(s) 38, 379, 497
BseYI CCCAGC 4 cut(s) 75, 356, 458, 488
BshFI GGCC 4 cut(s) 80, 310, 508, 730
BshNI GGYRCC 4 cut(s) 442, 664, 685, 694
BsiHKAI GWGCWC 2 cut(s) 48, 217
BsiHKCI CYCGRG 1 cut(s) 715
BsiSI CCGG 5 cut(s) 270, 277, 294, 311, 380
BslI CCNNNNNNNGG 4 cut(s) 181, 276, 332, 386
BsmAI GTCTC 2 cut(s) 20, 835
BsmBI CGTCTC 1 cut(s) 20
BsmI GAATGC 1 cut(s) 437
BsnI GGCC 4 cut(s) 80, 310, 508, 730
BsoBI CYCGRG 1 cut(s) 715
Bsp1286I GDGCHC 2 cut(s) 48, 217
Bsp1407I TGTACA 1 cut(s) 244
Bsp143I GATC 4 cut(s) 541, 569, 623, 672
BspACI CCGC 8 cut(s) 32, 65, 102, 144, 182, 425, 588, 704
BspANI GGCC 4 cut(s) 80, 310, 508, 730
BspCNI CTCAG 1 cut(s) 622
BspLI GGNNCC 5 cut(s) 135, 444, 666, 687, 696
BspMAI CTGCAG 1 cut(s) 763
BspPI GGATC 1 cut(s) 577
BspT107I GGYRCC 4 cut(s) 442, 664, 685, 694
BsrGI TGTACA 1 cut(s) 244
BsrI ACTGG 2 cut(s) 318, 731
BssECI CCNNGG 2 cut(s) 326, 714
BssMI GATC 4 cut(s) 541, 569, 623, 672
BssNI GRCGYC 1 cut(s) 443
Bst2UI CCWGG 2 cut(s) 176, 327
Bst4CI ACNGT 5 cut(s) 12, 233, 504, 812, 829
Bst6I CTCTTC 1 cut(s) 810
BstACI GRCGYC 1 cut(s) 443
BstAUI TGTACA 1 cut(s) 244
BstC8I GCNNGC 4 cut(s) 82, 114, 118, 122
BstDEI CTNAG 2 cut(s) 576, 630
BstEII GGTNACC 1 cut(s) 272
BstF5I GGATG 3 cut(s) 468, 687, 710
BstH2I RGCGCY 2 cut(s) 71, 446
BstHHI GCGC 3 cut(s) 56, 70, 445
BstKTI GATC 4 cut(s) 544, 572, 626, 675
BstMAI GTCTC 2 cut(s) 20, 835
BstMBI GATC 4 cut(s) 541, 569, 623, 672
BstMWI GCNNNNNNNGC 4 cut(s) 62, 81, 171, 727
BstNI CCWGG 2 cut(s) 176, 327
BstNSI RCATGY 1 cut(s) 157
BstPI GGTNACC 1 cut(s) 272
BstSCI CCNGG 2 cut(s) 174, 325
BstSFI CTRYAG 2 cut(s) 510, 759
BstV1I GCAGC 3 cut(s) 38, 379, 497
BstV2I GAAGAC 1 cut(s) 357
BstX2I RGATCY 1 cut(s) 672
BstYI RGATCY 1 cut(s) 672
BsuRI GGCC 4 cut(s) 80, 310, 508, 730
BtsCI GGATG 3 cut(s) 468, 687, 710
BtsI GCAGTG 1 cut(s) 768
BtsIMutI CAGTG 5 cut(s) 17, 509, 531, 768, 808
Cac8I GCNNGC 4 cut(s) 82, 114, 118, 122
CfoI GCGC 3 cut(s) 56, 70, 445
Cfr13I GGNCC 4 cut(s) 78, 94, 308, 497
CseI GACGC 1 cut(s) 384
Csp6I GTAC 4 cut(s) 245, 556, 695, 825
CviAII CATG 2 cut(s) 154, 559
CviQI GTAC 4 cut(s) 245, 556, 695, 825
DdeI CTNAG 2 cut(s) 576, 630
DinI GGCGCC 1 cut(s) 444
DpnI GATC 4 cut(s) 543, 571, 625, 674
DpnII GATC 4 cut(s) 541, 569, 623, 672
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
Eam1104I CTCTTC 1 cut(s) 810
EarI CTCTTC 1 cut(s) 810
EciI GGCGGA 3 cut(s) 47, 133, 440
Ecl136II GAGCTC 1 cut(s) 215
Eco24I GRGCYC 1 cut(s) 217
Eco32I GATATC 1 cut(s) 169
Eco47I GGWCC 2 cut(s) 94, 497
Eco53kI GAGCTC 1 cut(s) 215
Eco88I CYCGRG 1 cut(s) 715
Eco91I GGTNACC 1 cut(s) 272
EcoICRI GAGCTC 1 cut(s) 215
EcoO65I GGTNACC 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 174, 325
EcoRV GATATC 1 cut(s) 169
EcoT38I GRGCYC 1 cut(s) 217
EgeI GGCGCC 1 cut(s) 444
EheI GGCGCC 1 cut(s) 444
Esp3I CGTCTC 1 cut(s) 20
FaeI CATG 2 cut(s) 157, 562
FaiI YATR 6 cut(s) 155, 249, 512, 560, 656, 724
FatI CATG 2 cut(s) 153, 558
FauI CCCGC 1 cut(s) 711
Fnu4HI GCNGC 5 cut(s) 52, 66, 102, 393, 486
FokI GGATG 3 cut(s) 475, 694, 697
FriOI GRGCYC 1 cut(s) 217
Fsp4HI GCNGC 5 cut(s) 52, 66, 102, 393, 486
GlaI GCGC 3 cut(s) 55, 69, 444
GluI GCNGC 5 cut(s) 52, 66, 102, 393, 486
GsaI CCCAGC 4 cut(s) 79, 360, 462, 492
HaeII RGCGCY 2 cut(s) 71, 446
HaeIII GGCC 4 cut(s) 80, 310, 508, 730
HapII CCGG 5 cut(s) 270, 277, 294, 311, 380
HgaI GACGC 1 cut(s) 384
HhaI GCGC 3 cut(s) 56, 70, 445
Hin1I GRCGYC 1 cut(s) 443
Hin1II CATG 2 cut(s) 157, 562
Hin6I GCGC 3 cut(s) 54, 68, 443
HinP1I GCGC 3 cut(s) 54, 68, 443
HincII GTYRAC 1 cut(s) 815
HindII GTYRAC 1 cut(s) 815
HinfI GANTC 5 cut(s) 314, 383, 528, 608, 755
HpaII CCGG 5 cut(s) 270, 277, 294, 311, 380
HphI GGTGA 1 cut(s) 266
Hpy166II GTNNAC 5 cut(s) 97, 323, 500, 593, 815
Hpy188I TCNGA 6 cut(s) 546, 607, 631, 744, 754, 790
Hpy188III TCNNGA 1 cut(s) 773
Hpy8I GTNNAC 5 cut(s) 97, 323, 500, 593, 815
Hpy99I CGWCG 4 cut(s) 62, 89, 400, 843
HpyAV CCTTC 2 cut(s) 85, 708
HpyCH4III ACNGT 5 cut(s) 12, 233, 504, 812, 829
HpyCH4IV ACGT 4 cut(s) 18, 42, 777, 838
HpyCH4V TGCA 3 cut(s) 435, 654, 761
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 81, 171, 727
HpyF3I CTNAG 2 cut(s) 576, 630
HpySE526I ACGT 4 cut(s) 18, 42, 777, 838
Hsp92I GRCGYC 1 cut(s) 443
Hsp92II CATG 2 cut(s) 157, 562
HspAI GCGC 3 cut(s) 54, 68, 443
KasI GGCGCC 1 cut(s) 442
KpnI GGTACC 1 cut(s) 698
Kzo9I GATC 4 cut(s) 541, 569, 623, 672
LmnI GCTCC 1 cut(s) 634
Lsp1109I GCAGC 3 cut(s) 38, 379, 497
MaeII ACGT 4 cut(s) 18, 42, 777, 838
MaeIII GTNAC 2 cut(s) 14, 272
MalI GATC 4 cut(s) 543, 571, 625, 674
MboI GATC 4 cut(s) 541, 569, 623, 672
MboII GAAGA 4 cut(s) 16, 357, 360, 797
MfeI CAATTG 1 cut(s) 419
MflI RGATCY 1 cut(s) 672
MhlI GDGCHC 2 cut(s) 48, 217
MluCI AATT 4 cut(s) 297, 419, 615, 848
Mly113I GGCGCC 1 cut(s) 443
MlyI GAGTC 2 cut(s) 323, 377
MseI TTAA 2 cut(s) 300, 620
MspA1I CMGCKG 1 cut(s) 488
MspI CCGG 5 cut(s) 270, 277, 294, 311, 380
MspR9I CCNGG 2 cut(s) 176, 327
MunI CAATTG 1 cut(s) 419
Mva1269I GAATGC 1 cut(s) 437
MvaI CCWGG 2 cut(s) 176, 327
MwoI GCNNNNNNNGC 4 cut(s) 62, 81, 171, 727
NarI GGCGCC 1 cut(s) 443
NdeII GATC 4 cut(s) 541, 569, 623, 672
NlaIII CATG 2 cut(s) 157, 562
NlaIV GGNNCC 5 cut(s) 135, 444, 666, 687, 696
NmuCI GTSAC 1 cut(s) 272
NspI RCATGY 1 cut(s) 157
PcsI WCGNNNNNNNCGW 1 cut(s) 35
PctI GAATGC 1 cut(s) 437
PfeI GAWTC 3 cut(s) 528, 608, 755
PkrI GCNGC 5 cut(s) 53, 67, 103, 394, 487
PleI GAGTC 2 cut(s) 322, 377
PluTI GGCGCC 1 cut(s) 446
PpsI GAGTC 2 cut(s) 322, 377
Psp124BI GAGCTC 1 cut(s) 217
Psp6I CCWGG 2 cut(s) 174, 325
PspEI GGTNACC 1 cut(s) 272
PspFI CCCAGC 4 cut(s) 75, 356, 458, 488
PspGI CCWGG 2 cut(s) 174, 325
PspN4I GGNNCC 5 cut(s) 135, 444, 666, 687, 696
PspPI GGNCC 4 cut(s) 78, 94, 308, 497
PstI CTGCAG 1 cut(s) 763
PsuI RGATCY 1 cut(s) 672
PvuII CAGCTG 1 cut(s) 488
RsaI GTAC 4 cut(s) 246, 557, 696, 826
RsaNI GTAC 4 cut(s) 245, 556, 695, 825
SacI GAGCTC 1 cut(s) 217
SaqAI TTAA 2 cut(s) 300, 620
SatI GCNGC 5 cut(s) 52, 66, 102, 393, 486
Sau3AI GATC 4 cut(s) 541, 569, 623, 672
Sau96I GGNCC 4 cut(s) 78, 94, 308, 497
SchI GAGTC 2 cut(s) 323, 377
ScrFI CCNGG 2 cut(s) 176, 327
SduI GDGCHC 2 cut(s) 48, 217
SfcI CTRYAG 2 cut(s) 510, 759
SfoI GGCGCC 1 cut(s) 444
SinI GGWCC 2 cut(s) 94, 497
Sse9I AATT 4 cut(s) 297, 419, 615, 848
SsiI CCGC 8 cut(s) 32, 65, 102, 144, 182, 425, 588, 704
SspDI GGCGCC 1 cut(s) 442
SstI GAGCTC 1 cut(s) 217
StyD4I CCNGG 2 cut(s) 174, 325
TaaI ACNGT 5 cut(s) 12, 233, 504, 812, 829
TaiI ACGT 4 cut(s) 21, 45, 780, 841
TaqI TCGA 2 cut(s) 398, 540
TasI AATT 4 cut(s) 297, 419, 615, 848
TatI WGTACW 1 cut(s) 244
TauI GCSGC 2 cut(s) 68, 104
TfiI GAWTC 3 cut(s) 528, 608, 755
Tru1I TTAA 2 cut(s) 300, 620
Tru9I TTAA 2 cut(s) 300, 620
TscAI CASTG 5 cut(s) 17, 509, 538, 768, 815
TseFI GTSAC 1 cut(s) 272
TseI GCWGC 3 cut(s) 51, 392, 485
Tsp45I GTSAC 1 cut(s) 272
TspDTI ATGAA 2 cut(s) 17, 600
TspRI CASTG 5 cut(s) 17, 509, 538, 768, 815
VpaK11BI GGWCC 2 cut(s) 94, 497
XceI RCATGY 1 cut(s) 157
XcmI CCANNNNNNNNNTGG 2 cut(s) 182, 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.