Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001314

Physical Location & Seq

Genomic Coordinates Forward (+)

33830125 .. 33843647

Gene Structure 13523 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1250300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2790 bp

ATGCCTGGGAACAAGTACAACGGTAATTCAGCCCACATCCCACCAACTCGAATTGAAAGGCTCTTGAGAGAAAGGGAGCTAAGGAAAAATATCAAGGCCTTGAACTTGAATGAAACATTTGAACCTGAGTTGCAGTTAAGAGAAGATGAAAACTCAAAAGCTGCCCTTGTTGAACGATTCTTGGAAGGGGCTGCAGCTGCAAGGGCGCTTAATGAGGGATGCGAAAAGCAAGACGGGAGGCCTAGCAGACAACGACTGTTGGTTGTGGCGAACAGGTTACCTGTCTCTGCAATTAGGAGAGGTGAAGATTCATGGTCTCTGGAGATGAGTGCAGGGGGTCTGGTCAGTGCACTTTTGGGTGTAAAGGAGTTTGAGGCAAGGTGGATTGGTTGGGCCGGGGTAAATGTGCCGGATGAAATTGGACAGAAGGCGCTTACTAGAGCTCTTGCTGAGAAGAGATGTATTCCAGTTTTCCTTGACGAGGAGATTGTTCATCAATATTACAATGGCTATTGCAACAATATTTTGTGGCCCCTTTTCCATTACCTTGGACTTCCACAAGAAGATCGACTTGCCACCACCCGGAGTTTCCAGTCACAGTTTGCTGCATATAAGAAGGCAAATCAAATGTTTGCTGATGTAGTTAATGAGCACTATGAAGAGGGTGACGTAGTTTGGTGCCATGATTACCATCTTATGTTTCTTCCAAAATGCTTGAAGGATTACGATAGTAAAATGAAAGTTGGTTGGTTTCTCCACACCCCTTTTCCTTCTTCTGAAATCCATAGGACATTGCCATCACGATCAGAACTGCTACGTGCAGTTCTTGCAGCTGATTTAGTTGGTTTTCACACTTATGACTATGCACGGCACTTTGTTAGTGCTTGCACTCGCATTCTTGGACTTGAGGGTACACCTGAAGGAGTTGAGGATCAAGGGAGGCTTACTCGAGTGGCTGCGTTTCCTATTGGAATAGATTCAGACCGCTTCATACGTGGACTTGAGGATCCTCAAGTCCAAGAACACATTAAGAAAATGAAGGAACGGTTGGCTGGGAGAAAGGTAATGCTAGGTGTTGATCGCCTTGATATGATTAAAGGAATTCCTCAAAAGTTACTGGCGTTTGAAAAGTTTCTTGAGGAAAACCCAACTTGGCGTGACAAAGTGGTTTTGCTGCAAATTGCAGTGCCAACAAGAACAGATGTTCCAGAGTATCAAAAACTTACAAGCCAGGTTCATGAAATTGTTGGGAGAATTAATGGCAGATTTGGAACTTTGACCGCTGTCCCAATACATCACCTGGATTGTTCTCTCGACTTTCATGAATTGTGTGCACTATATGCTGTAACTGATGTAGCACTTGTCACATCCCTGAGGGACGGAATGAATCTTGTCAGTTATGAGTTTGTGGCATGCCAGGATGCAAAGAGAGGGGTCCTTATTCTGAGTGAGTTTGCTGGTGCCGCACAGTCTTTAGGTGCTGGAGCAATTCTAGTGAACCCGTGGAACATCACAGAAGTTGCTAATTCAATTGCTCAAGCATTGAATATGGACTCCGAACAAAGAGAGAAGCGACATAAGCATAATTTTTTACACGTGACAGCCCACACTGCACAGGAATGGGCCGAAACTTTTGTAAGTGAACTGAACGATACTGTTGTAGCGGCGCAATTACGGACCAGACAGGTGCCACCACCACTTCCAAACAAGGATGCAATTCAAAGTTATTTGCAAGCAAATAATCGATTGATCATACTGGGATTCAATGCAACATTAACTGAATCAGTGGACACACATGAGAGGCGAGGTGATCAAATAAAAGAAATGGAACTTAAACTGCACCCCAAATTGAAAGAAACCTTGTCAGCACTCTGCAATGATCCAACAACAACTATTGTCGTCCTCAGTGGGAGTGAGAGACATGTCTTAGATGATAACTTTGGGGAGCTTGACATGTGGTTGGCAGCTGAAAACGGGATGTTTTTACGCCTTACAAAGGGAGAATGGATGACAACAATGCCTGAACATGTGAATATGGAATGGGTCAACAGTGTGGAGCATGTTTTTGAGTATTTCAAAGAGAGAACACCGCGATCACATTATGAACGTCGTCAAACTTCCCTTGTGTGGAATTATAAATATGCAGATATTGATTTTGGGAGACTTCAAGCCAGAGATATGCTGCAGCATCTCTGGACTGGCCCAATTTCTAATGCCTCTGTTGATGTTGTTCAAGGTAGCCGGTCTGTTGAGGTTCGAGCAGTTGGTGTGACAAAGGGTGCAGCAATTGACCGTATATTGGGAGAGATAGTTCATAGTAAATCCATGACATCCCCCATTGATTATGTGCTGTGTATTGGACATTTTCTTGGGAAGGATGAAGATGTGTACACCTTTTTCGAGCCAGATCTTCCGCCTGATCCGAGTAGTCTTCCAAGAACCAAGATACCTGATGGACTAAAGCCTAACGAGAGGCGATCTTCTTTGAAGGCTCCGGCCATCAAAAGCGGCTCAAAATCGTCTCAGAGCAAAGCGCAACGGGCGCCTTTACCAAACCACGAGAAGAAAAATGCAAATCACAACACCACGAATCCACGATGGCCATCTCCAGAAAAGGTTTCGTGGAACGTACTTGATCTAAAGAAGGAGAACTACTACTCTTGCGCTGTCGGGCGGACTCGCACAAATGCTCGGTATCTACTTCAGTCCTCGGATGATGTTGTCTCGTTTCTGAAGGAATTGGCGGGTGCCTCTTCTGCAAGCGTTTCATCTTCCGGTTCAGAGATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003825 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009311 GO:0009312 GO:0009743 GO:0009756 GO:0009790 GO:0009791 GO:0009793 GO:0009832 GO:0009987 GO:0010033 GO:0010154 GO:0010182 GO:0016051 GO:0016311 GO:0016740 GO:0016757 GO:0016758 GO:0016787 GO:0016788 GO:0016791 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0033554 GO:0034637 GO:0035251 GO:0042221 GO:0042546 GO:0042578 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0044424 GO:0044464 GO:0046351 GO:0046527 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051301 GO:0051716 GO:0061458 GO:0065007 GO:0070413 GO:0070887 GO:0071310 GO:0071322 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

930

Amino Acids

104.79

Weight (kDa)

6.46

Isoelectric Point (pI)

46.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_transf_20	PF00982	85 - 550	6.6e-189	Glycosyltransferase family 20
Trehalose_PPase	PF02358	606 - 804	2.1e-59	Trehalose-phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001314)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78580 AT1G78580 AT1G78580 AT1G78580
fragaria_vesca	FvH4_3g06590 FvH4_3g06590 FvH4_3g17650 FvH4_3g17650
malus_domestica	MD03G1250300.v1.1 MD10G1270400.v1.1 MD11G1270400.v1.1
prunus_persica	Prupe.4G071400_v2.0.a1 Prupe.4G071400_v2.0.a1 Prupe.4G071400_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1
pyrus_communis	pycom03g19800 pycom10g22630 pycom11g23920
rosa_chinensis	RchiOBHm_Chr5g0012561 RchiOBHm_Chr5g0029381
rosa_laevigata	RLG00000031925 RLG00000033160
rosa_multiflora	Rmu_sc0003577.1_g000012 Rmu_sc0004373.1_g000021
rosa_roxburghii	Rroxscaffold_1G00050580 Rroxscaffold_1G00064100
rosa_rugosa	Rorug05G0002400 Rorug05G0002400 Rorug05G0002500 Rorug05G0002600 Rorug05G0114000 Rorug05G0114000 Rorug05G0114000 Rorug05G0114000 Rorug05G0114000
rosa_samantha	Rh5AG096400 Rh5AG205600 Rh5BG092600 Rh5BG204200 Rh5CG105500 Rh5DG092100 Rh5DG207800
rosa_wichuraiana	Rw5G008440 Rw5G018730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2136
AccB1I	GGYRCC	5 cut(s)	678, 1460, 1687, 2542, 2747
AccII	CGCG	1 cut(s)	2092
AciI	CCGC	9 cut(s)	985, 1281, 1464, 1664, 2090, 2414, 2508, 2674, 2744
AclWI	GGATC	5 cut(s)	939, 1001, 1014, 1874, 2414
AcoI	YGGCCR	2 cut(s)	2496, 2600
AcsI	RAATTY	1 cut(s)	1101
AcuI	CTGAAG	3 cut(s)	939, 2687, 2753
AcvI	CACGTG	1 cut(s)	1597
AcyI	GRCGYC	1 cut(s)	2543
AfaI	GTAC	4 cut(s)	17, 913, 2390, 2631
AfiI	CCNNNNNNNGG	5 cut(s)	481, 582, 1996, 2127, 2299
AflIII	ACRYGT	4 cut(s)	1594, 1921, 1953, 2026
AjnI	CCWGG	4 cut(s)	4, 1230, 1299, 1416
AjuI	GAANNNNNNNTTGG	2 cut(s)	1031, 1063
AloI	GAACNNNNNNTCC	4 cut(s)	1189, 1221, 2295, 2327
AluBI	AGCT	7 cut(s)	79, 161, 197, 443, 833, 1948, 1967
AluI	AGCT	7 cut(s)	79, 161, 197, 443, 833, 1948, 1967
Alw21I	GWGCWC	4 cut(s)	352, 445, 654, 1336
Alw26I	GTCTC	6 cut(s)	289, 321, 1912, 2155, 2526, 2728
Alw44I	GTGCAC	2 cut(s)	348, 1332
AlwI	GGATC	5 cut(s)	939, 1001, 1014, 1874, 2414
Ama87I	CYCGRG	1 cut(s)	948
AoxI	GGCC	8 cut(s)	96, 239, 393, 530, 1623, 2200, 2496, 2600
ApaLI	GTGCAC	2 cut(s)	348, 1332
ApoI	RAATTY	1 cut(s)	1101
AseI	ATTAAT	1 cut(s)	1257
Asp700I	GAANNNNTTC	2 cut(s)	976, 1131
AspLEI	GCGC	6 cut(s)	208, 433, 1669, 2536, 2545, 2666
AspS9I	GGNCC	6 cut(s)	393, 531, 1435, 1623, 1677, 2201
AsuC2I	CCSGG	2 cut(s)	397, 583
AsuHPI	GGTGA	4 cut(s)	314, 677, 1289, 1820
AvaI	CYCGRG	1 cut(s)	948
AvaII	GGWCC	2 cut(s)	1435, 1677
AxyI	CCTNAGG	1 cut(s)	1373
BaeGI	GKGCMC	2 cut(s)	352, 1336
BaeI	ACNNNNGTAYC	2 cut(s)	1644, 1677
BalI	TGGCCA	1 cut(s)	2602
BamHI	GGATCC	1 cut(s)	1006
BanI	GGYRCC	5 cut(s)	678, 1460, 1687, 2542, 2747
BanII	GRGCYC	1 cut(s)	445
BarI	GAAGNNNNNNTAC	2 cut(s)	2636, 2668
BauI	CACGAG	1 cut(s)	2558
BbrPI	CACGTG	1 cut(s)	1597
BbsI	GAAGAC	1 cut(s)	2423
Bbv12I	GWGCWC	4 cut(s)	352, 445, 654, 1336
BccI	CCATC	6 cut(s)	699, 805, 2447, 2507, 2592, 2611
BceAI	ACGGC	1 cut(s)	884
BciT130I	CCWGG	4 cut(s)	6, 1232, 1301, 1418
BclI	TGATCA	2 cut(s)	1749, 1810
BcnI	CCSGG	2 cut(s)	397, 583
BcoDI	GTCTC	6 cut(s)	289, 321, 1912, 2155, 2526, 2728
BfaI	CTAG	4 cut(s)	243, 438, 1070, 1493
BfmI	CTRYAG	2 cut(s)	192, 2183
BfoI	RGCGCY	3 cut(s)	209, 434, 2546
BglII	AGATCT	1 cut(s)	2407
Bme1390I	CCNGG	6 cut(s)	6, 397, 583, 1232, 1301, 1418
Bme18I	GGWCC	2 cut(s)	1435, 1677
BmeT110I	CYCGRG	1 cut(s)	948
BmgT120I	GGNCC	6 cut(s)	393, 531, 1435, 1623, 1677, 2201
BmiI	GGNNCC	9 cut(s)	533, 680, 1008, 1436, 1462, 1689, 2493, 2544, 2749
BmrFI	CCNGG	6 cut(s)	6, 397, 583, 1232, 1301, 1418
BmrI	ACTGGG	1 cut(s)	1766
BmsI	GCATC	4 cut(s)	209, 1411, 1702, 2197
BmuI	ACTGGG	1 cut(s)	1766
BoxI	GACNNNNGTC	1 cut(s)	1283
BpiI	GAAGAC	1 cut(s)	2423
BplI	GAGNNNNNCTC	2 cut(s)	931, 963
BpmI	CTGGAG	3 cut(s)	341, 1503, 2592
Bpu10I	CCTNAGC	1 cut(s)	80
BpuEI	CTTGAG	6 cut(s)	85, 926, 996, 1022, 1157, 1521
BpuMI	CCSGG	2 cut(s)	397, 583
Bsa29I	ATCGAT	1 cut(s)	1744
BsaAI	YACGTR	3 cut(s)	818, 995, 1597
BsaBI	GATNNNNATC	2 cut(s)	690, 2602
BsaHI	GRCGYC	1 cut(s)	2543
BsaI	GGTCTC	1 cut(s)	321
BsaJI	CCNNGG	5 cut(s)	5, 396, 547, 1502, 2709
BsaWI	WCCGGW	1 cut(s)	2774
BsaXI	ACNNNNNCTCC	2 cut(s)	2295, 2325
Bsc4I	CCNNNNNNNGG	5 cut(s)	481, 582, 1996, 2127, 2299
Bse118I	RCCGGY	1 cut(s)	2241
Bse1I	ACTGG	5 cut(s)	467, 592, 1122, 1761, 2203
Bse21I	CCTNAGG	1 cut(s)	1373
Bse3DI	GCAATG	2 cut(s)	791, 1882
Bse8I	GATNNNNATC	2 cut(s)	690, 2602
BseBI	CCWGG	4 cut(s)	6, 1232, 1301, 1418
BseCI	ATCGAT	1 cut(s)	1744
BseDI	CCNNGG	5 cut(s)	5, 396, 547, 1502, 2709
BseJI	GATNNNNATC	2 cut(s)	690, 2602
BseLI	CCNNNNNNNGG	5 cut(s)	481, 582, 1996, 2127, 2299
BseMI	GCAATG	2 cut(s)	791, 1882
BseMII	CTCAG	6 cut(s)	117, 441, 1364, 1436, 1918, 2537
BseNI	ACTGG	5 cut(s)	467, 592, 1122, 1761, 2203
BseRI	GAGGAG	1 cut(s)	497
BseSI	GKGCMC	2 cut(s)	352, 1336
BseYI	CCCAGC	1 cut(s)	1052
BsgI	GTGCAG	5 cut(s)	351, 840, 1596, 1823, 2301
Bsh1236I	CGCG	1 cut(s)	2092
BshFI	GGCC	8 cut(s)	98, 241, 395, 532, 1625, 2202, 2498, 2602
BshNI	GGYRCC	5 cut(s)	678, 1460, 1687, 2542, 2747
BshVI	ATCGAT	1 cut(s)	1744
BsiHKAI	GWGCWC	4 cut(s)	352, 445, 654, 1336
BsiHKCI	CYCGRG	1 cut(s)	948
BsiSI	CCGG	6 cut(s)	396, 410, 583, 2242, 2495, 2775
BslFI	GGGAC	2 cut(s)	1271, 1391
BslI	CCNNNNNNNGG	5 cut(s)	481, 582, 1996, 2127, 2299
BsmAI	GTCTC	6 cut(s)	289, 321, 1912, 2155, 2526, 2728
BsmBI	CGTCTC	1 cut(s)	2526
BsmFI	GGGAC	2 cut(s)	1271, 1391
BsmI	GAATGC	1 cut(s)	894
BsnI	GGCC	8 cut(s)	98, 241, 395, 532, 1625, 2202, 2498, 2602
Bso31I	GGTCTC	1 cut(s)	321
BsoBI	CYCGRG	1 cut(s)	948
Bsp1286I	GDGCHC	4 cut(s)	352, 445, 654, 1336
Bsp1407I	TGTACA	1 cut(s)	2388
BspACI	CCGC	9 cut(s)	985, 1281, 1464, 1664, 2090, 2414, 2508, 2674, 2744
BspANI	GGCC	8 cut(s)	98, 241, 395, 532, 1625, 2202, 2498, 2602
BspCNI	CTCAG	6 cut(s)	118, 442, 1365, 1437, 1917, 2536
BspDI	ATCGAT	1 cut(s)	1744
BspFNI	CGCG	1 cut(s)	2092
BspHI	TCATGA	2 cut(s)	1237, 1321
BspLI	GGNNCC	9 cut(s)	533, 680, 1008, 1436, 1462, 1689, 2493, 2544, 2749
BspMAI	CTGCAG	2 cut(s)	196, 2187
BspPI	GGATC	5 cut(s)	939, 1001, 1014, 1874, 2414
BspT107I	GGYRCC	5 cut(s)	678, 1460, 1687, 2542, 2747
BspTNI	GGTCTC	1 cut(s)	321
BsrDI	GCAATG	2 cut(s)	791, 1882
BsrFI	RCCGGY	1 cut(s)	2241
BsrGI	TGTACA	1 cut(s)	2388
BsrI	ACTGG	5 cut(s)	467, 592, 1122, 1761, 2203
BssAI	RCCGGY	1 cut(s)	2241
BssECI	CCNNGG	5 cut(s)	5, 396, 547, 1502, 2709
BssNI	GRCGYC	1 cut(s)	2543
BssSI	CACGAG	1 cut(s)	2558
BssT1I	CCWWGG	1 cut(s)	547
Bst2BI	CACGAG	1 cut(s)	2558
Bst2UI	CCWGG	4 cut(s)	6, 1232, 1301, 1418
Bst4CI	ACNGT	8 cut(s)	23, 258, 600, 1047, 1470, 1657, 2051, 2294
Bst6I	CTCTTC	3 cut(s)	449, 654, 2758
BstACI	GRCGYC	1 cut(s)	2543
BstAPI	GCANNNNNTGC	2 cut(s)	827, 1340
BstAUI	TGTACA	1 cut(s)	2388
BstBAI	YACGTR	3 cut(s)	818, 995, 1597
BstC8I	GCNNGC	4 cut(s)	886, 1414, 1734, 2761
BstDEI	CTNAG	8 cut(s)	80, 126, 450, 1373, 1445, 1904, 1927, 2523
BstDSI	CCRYGG	1 cut(s)	1502
BstEII	GGTNACC	1 cut(s)	276
BstENI	CCTNNNNNAGG	2 cut(s)	479, 1994
BstFNI	CGCG	1 cut(s)	2092
BstH2I	RGCGCY	3 cut(s)	209, 434, 2546
BstHHI	GCGC	6 cut(s)	208, 433, 1669, 2536, 2545, 2666
BstMAI	GTCTC	6 cut(s)	289, 321, 1912, 2155, 2526, 2728
BstNI	CCWGG	4 cut(s)	6, 1232, 1301, 1418
BstNSI	RCATGY	5 cut(s)	1416, 1925, 1957, 2030, 2063
BstPAI	GACNNNNGTC	1 cut(s)	1283
BstPI	GGTNACC	1 cut(s)	276
BstSCI	CCNGG	6 cut(s)	4, 395, 581, 1230, 1299, 1416
BstSFI	CTRYAG	2 cut(s)	192, 2183
BstSLI	GKGCMC	2 cut(s)	352, 1336
BstUI	CGCG	1 cut(s)	2092
BstV2I	GAAGAC	1 cut(s)	2423
BstX2I	RGATCY	2 cut(s)	1006, 2407
BstXI	CCANNNNNNTGG	1 cut(s)	548
BstYI	RGATCY	2 cut(s)	1006, 2407
Bsu15I	ATCGAT	1 cut(s)	1744
Bsu36I	CCTNAGG	1 cut(s)	1373
BsuRI	GGCC	8 cut(s)	98, 241, 395, 532, 1625, 2202, 2498, 2602
BsuTUI	ATCGAT	1 cut(s)	1744
BtgI	CCRYGG	1 cut(s)	1502
BtsI	GCAGTG	2 cut(s)	1191, 1608
BtsIMutI	CAGTG	6 cut(s)	352, 1191, 1608, 1791, 1912, 2056
Cac8I	GCNNGC	4 cut(s)	886, 1414, 1734, 2761
CciI	TCATGA	2 cut(s)	1237, 1321
CfoI	GCGC	6 cut(s)	208, 433, 1669, 2536, 2545, 2666
Cfr10I	RCCGGY	1 cut(s)	2241
Cfr13I	GGNCC	6 cut(s)	393, 531, 1435, 1623, 1677, 2201
ClaI	ATCGAT	1 cut(s)	1744
Csp6I	GTAC	4 cut(s)	16, 912, 2389, 2630
CviQI	GTAC	4 cut(s)	16, 912, 2389, 2630
DdeI	CTNAG	8 cut(s)	80, 126, 450, 1373, 1445, 1904, 1927, 2523
DinI	GGCGCC	1 cut(s)	2544
EaeI	YGGCCR	2 cut(s)	2496, 2600
Eam1104I	CTCTTC	3 cut(s)	449, 654, 2758
EarI	CTCTTC	3 cut(s)	449, 654, 2758
EciI	GGCGGA	2 cut(s)	2403, 2689
Ecl136II	GAGCTC	1 cut(s)	443
Eco130I	CCWWGG	1 cut(s)	547
Eco147I	AGGCCT	2 cut(s)	98, 241
Eco24I	GRGCYC	1 cut(s)	445
Eco31I	GGTCTC	1 cut(s)	321
Eco47I	GGWCC	2 cut(s)	1435, 1677
Eco53kI	GAGCTC	1 cut(s)	443
Eco57I	CTGAAG	3 cut(s)	939, 2687, 2753
Eco72I	CACGTG	1 cut(s)	1597
Eco81I	CCTNAGG	1 cut(s)	1373
Eco88I	CYCGRG	1 cut(s)	948
Eco91I	GGTNACC	1 cut(s)	276
EcoICRI	GAGCTC	1 cut(s)	443
EcoNI	CCTNNNNNAGG	2 cut(s)	479, 1994
EcoO109I	RGGNCCY	1 cut(s)	1435
EcoO65I	GGTNACC	1 cut(s)	276
EcoRI	GAATTC	1 cut(s)	1101
EcoRII	CCWGG	4 cut(s)	4, 1230, 1299, 1416
EcoT14I	CCWWGG	1 cut(s)	547
EcoT38I	GRGCYC	1 cut(s)	445
EgeI	GGCGCC	1 cut(s)	2544
EheI	GGCGCC	1 cut(s)	2544
ErhI	CCWWGG	1 cut(s)	547
Esp3I	CGTCTC	1 cut(s)	2526
FalI	AAGNNNNNCTT	6 cut(s)	150, 182, 555, 587, 927, 959
FaqI	GGGAC	2 cut(s)	1271, 1391
FauI	CCCGC	1 cut(s)	2737
FbaI	TGATCA	2 cut(s)	1749, 1810
FriOI	GRGCYC	1 cut(s)	445
FspBI	CTAG	4 cut(s)	243, 438, 1070, 1493
GlaI	GCGC	6 cut(s)	207, 432, 1668, 2535, 2544, 2665
GsaI	CCCAGC	1 cut(s)	1056
GsuI	CTGGAG	3 cut(s)	341, 1503, 2592
HaeII	RGCGCY	3 cut(s)	209, 434, 2546
HaeIII	GGCC	8 cut(s)	98, 241, 395, 532, 1625, 2202, 2498, 2602
HapII	CCGG	6 cut(s)	396, 410, 583, 2242, 2495, 2775
HhaI	GCGC	6 cut(s)	208, 433, 1669, 2536, 2545, 2666
Hin1I	GRCGYC	1 cut(s)	2543
Hin6I	GCGC	6 cut(s)	206, 431, 1667, 2534, 2543, 2664
HinP1I	GCGC	6 cut(s)	206, 431, 1667, 2534, 2543, 2664
HincII	GTYRAC	1 cut(s)	2047
HindII	GTYRAC	1 cut(s)	2047
HinfI	GANTC	9 cut(s)	177, 308, 977, 1387, 1553, 1761, 1781, 2590, 2677
HpaII	CCGG	6 cut(s)	396, 410, 583, 2242, 2495, 2775
HphI	GGTGA	4 cut(s)	314, 677, 1289, 1820
Hpy99I	CGWCG	1 cut(s)	2112
HpyCH4III	ACNGT	8 cut(s)	23, 258, 600, 1047, 1470, 1657, 2051, 2294
HpyCH4IV	ACGT	6 cut(s)	669, 817, 994, 1596, 2107, 2628
HpyF3I	CTNAG	8 cut(s)	80, 126, 450, 1373, 1445, 1904, 1927, 2523
HpySE526I	ACGT	6 cut(s)	669, 817, 994, 1596, 2107, 2628
Hsp92I	GRCGYC	1 cut(s)	2543
HspAI	GCGC	6 cut(s)	206, 431, 1667, 2534, 2543, 2664
KasI	GGCGCC	1 cut(s)	2542
Ksp22I	TGATCA	2 cut(s)	1749, 1810
LmnI	GCTCC	5 cut(s)	76, 1484, 1945, 2056, 2497
LweI	GCATC	4 cut(s)	209, 1411, 1702, 2197
MaeI	CTAG	4 cut(s)	243, 438, 1070, 1493
MaeII	ACGT	6 cut(s)	669, 817, 994, 1596, 2107, 2628
MaeIII	GTNAC	9 cut(s)	276, 594, 665, 1113, 1157, 1345, 1363, 1597, 2269
MfeI	CAATTG	2 cut(s)	1530, 2286
MflI	RGATCY	2 cut(s)	1006, 2407
MhlI	GDGCHC	4 cut(s)	352, 445, 654, 1336
MlsI	TGGCCA	1 cut(s)	2602
MluNI	TGGCCA	1 cut(s)	2602
Mly113I	GGCGCC	1 cut(s)	2543
MlyI	GAGTC	2 cut(s)	1547, 2671
MmeI	TCCRAC	1 cut(s)	1907
Mox20I	TGGCCA	1 cut(s)	2602
MroXI	GAANNNNTTC	2 cut(s)	976, 1131
MscI	TGGCCA	1 cut(s)	2602
MseI	TTAA	8 cut(s)	137, 210, 645, 1029, 1095, 1257, 1775, 1833
MslI	CAYNNNNRTG	2 cut(s)	855, 1618
Msp20I	TGGCCA	1 cut(s)	2602
MspA1I	CMGCKG	4 cut(s)	197, 833, 1283, 1967
MspI	CCGG	6 cut(s)	396, 410, 583, 2242, 2495, 2775
MspR9I	CCNGG	6 cut(s)	6, 397, 583, 1232, 1301, 1418
MunI	CAATTG	2 cut(s)	1530, 2286
Mva1269I	GAATGC	1 cut(s)	894
MvaI	CCWGG	4 cut(s)	6, 1232, 1301, 1418
MvnI	CGCG	1 cut(s)	2092
NarI	GGCGCC	1 cut(s)	2543
NciI	CCSGG	2 cut(s)	397, 583
NlaIV	GGNNCC	9 cut(s)	533, 680, 1008, 1436, 1462, 1689, 2493, 2544, 2749
NmuCI	GTSAC	6 cut(s)	594, 665, 1157, 1363, 1597, 2269
NspI	RCATGY	5 cut(s)	1416, 1925, 1957, 2030, 2063
PaeI	GCATGC	1 cut(s)	1416
PaeR7I	CTCGAG	1 cut(s)	948
PagI	TCATGA	2 cut(s)	1237, 1321
PceI	AGGCCT	2 cut(s)	98, 241
PciI	ACATGT	3 cut(s)	1921, 1953, 2026
PctI	GAATGC	1 cut(s)	894
PdmI	GAANNNNTTC	2 cut(s)	976, 1131
PfeI	GAWTC	7 cut(s)	177, 308, 977, 1387, 1761, 1781, 2590
PleI	GAGTC	2 cut(s)	1547, 2671
PluTI	GGCGCC	1 cut(s)	2546
PmaCI	CACGTG	1 cut(s)	1597
PmlI	CACGTG	1 cut(s)	1597
PpsI	GAGTC	2 cut(s)	1547, 2671
Ppu21I	YACGTR	3 cut(s)	818, 995, 1597
PpuMI	RGGWCCY	1 cut(s)	1435
PscI	ACATGT	3 cut(s)	1921, 1953, 2026
PshAI	GACNNNNGTC	1 cut(s)	1283
PshBI	ATTAAT	1 cut(s)	1257
PsiI	TTATAA	1 cut(s)	2136
Psp124BI	GAGCTC	1 cut(s)	445
Psp5II	RGGWCCY	1 cut(s)	1435
Psp6I	CCWGG	4 cut(s)	4, 1230, 1299, 1416
PspCI	CACGTG	1 cut(s)	1597
PspEI	GGTNACC	1 cut(s)	276
PspFI	CCCAGC	1 cut(s)	1052
PspGI	CCWGG	4 cut(s)	4, 1230, 1299, 1416
PspN4I	GGNNCC	9 cut(s)	533, 680, 1008, 1436, 1462, 1689, 2493, 2544, 2749
PspPI	GGNCC	6 cut(s)	393, 531, 1435, 1623, 1677, 2201
PspPPI	RGGWCCY	1 cut(s)	1435
PspXI	VCTCGAGB	1 cut(s)	948
PstI	CTGCAG	2 cut(s)	196, 2187
PsuI	RGATCY	2 cut(s)	1006, 2407
PvuII	CAGCTG	3 cut(s)	197, 833, 1967
RsaI	GTAC	4 cut(s)	17, 913, 2390, 2631
RsaNI	GTAC	4 cut(s)	16, 912, 2389, 2630
RseI	CAYNNNNRTG	2 cut(s)	855, 1618
SacI	GAGCTC	1 cut(s)	445
SaqAI	TTAA	8 cut(s)	137, 210, 645, 1029, 1095, 1257, 1775, 1833
Sau96I	GGNCC	6 cut(s)	393, 531, 1435, 1623, 1677, 2201
SchI	GAGTC	2 cut(s)	1547, 2671
ScrFI	CCNGG	6 cut(s)	6, 397, 583, 1232, 1301, 1418
SduI	GDGCHC	4 cut(s)	352, 445, 654, 1336
SfaNI	GCATC	4 cut(s)	209, 1411, 1702, 2197
SfcI	CTRYAG	2 cut(s)	192, 2183
SfoI	GGCGCC	1 cut(s)	2544
Sfr274I	CTCGAG	1 cut(s)	948
SinI	GGWCC	2 cut(s)	1435, 1677
SlaI	CTCGAG	1 cut(s)	948
SmiMI	CAYNNNNRTG	2 cut(s)	855, 1618
SmlI	CTYRAG	7 cut(s)	64, 905, 948, 1001, 1011, 1136, 1536
SmoI	CTYRAG	7 cut(s)	64, 905, 948, 1001, 1011, 1136, 1536
SphI	GCATGC	1 cut(s)	1416
SseBI	AGGCCT	2 cut(s)	98, 241
SsiI	CCGC	9 cut(s)	985, 1281, 1464, 1664, 2090, 2414, 2508, 2674, 2744
SspDI	GGCGCC	1 cut(s)	2542
SspI	AATATT	2 cut(s)	500, 523
SspMI	CTAG	4 cut(s)	243, 438, 1070, 1493
SstI	GAGCTC	1 cut(s)	445
StuI	AGGCCT	2 cut(s)	98, 241
StyD4I	CCNGG	6 cut(s)	4, 395, 581, 1230, 1299, 1416
StyI	CCWWGG	1 cut(s)	547
TaaI	ACNGT	8 cut(s)	23, 258, 600, 1047, 1470, 1657, 2051, 2294
TaiI	ACGT	6 cut(s)	672, 820, 997, 1599, 2110, 2631
TaqI	TCGA	7 cut(s)	49, 568, 949, 1314, 1744, 2257, 2400
TatI	WGTACW	2 cut(s)	15, 2388
TauI	GCSGC	3 cut(s)	1466, 1667, 2511
TfiI	GAWTC	7 cut(s)	177, 308, 977, 1387, 1761, 1781, 2590
Tru1I	TTAA	8 cut(s)	137, 210, 645, 1029, 1095, 1257, 1775, 1833
Tru9I	TTAA	8 cut(s)	137, 210, 645, 1029, 1095, 1257, 1775, 1833
TscAI	CASTG	6 cut(s)	352, 1191, 1615, 1791, 1912, 2056
TseFI	GTSAC	6 cut(s)	594, 665, 1157, 1363, 1597, 2269
Tsp45I	GTSAC	6 cut(s)	594, 665, 1157, 1363, 1597, 2269
TspGWI	ACGGA	2 cut(s)	1395, 1690
TspRI	CASTG	6 cut(s)	352, 1191, 1615, 1791, 1912, 2056
VneI	GTGCAC	2 cut(s)	348, 1332
VpaK11BI	GGWCC	2 cut(s)	1435, 1677
VspI	ATTAAT	1 cut(s)	1257
XagI	CCTNNNNNAGG	2 cut(s)	479, 1994
XapI	RAATTY	1 cut(s)	1101
XceI	RCATGY	5 cut(s)	1416, 1925, 1957, 2030, 2063
XhoI	CTCGAG	1 cut(s)	948
XmnI	GAANNNNTTC	2 cut(s)	976, 1131
XspI	CTAG	4 cut(s)	243, 438, 1070, 1493

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.