Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0001314

Physical Location & Seq

Genomic Coordinates Reverse (-)

24954055 .. 24960213

Gene Structure 6159 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g22630.14

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2790 bp

ATGCCAGGAAATAATTATAACTGCAATCCTTCCACACCAAGAAGTAGATTGGAAAGGCTTCTCAGGGAAAGAGAGCTAAGGAGATCAAACCGCGTTTCACAATCAAATGAAGATGGAAACAGACAGGCAGAATTCGTAGGGAGTGATGCATTTTTCACCGAGGGTGAAAATTTAGGTTTATCCAGTGAGGACGATTTTCCGGAAGGAGTTTCAGCAACAAAAATTTTTATTGATGGATGTGAATGGAAGGAGGGACGGCCTCCTAAACAGCGTTTACTGGTGGTGGCTAATAGGTTACCTGTCTCTGCAGTTAGGAAGGGAGAGGACTCATGGCAGCTTGAGATAAGTGTCGGAGGACTAGTCAGTGCACTTTTAGGCGTAAAGGAGTTCGATGCCAGGTGGATTGGTTGGGCTGGTGTAAATGTACCAGATAGTGTTGGACAAAAAGCATTGACTAAAGCTTTAGCTGAAAAGAGGTGCATCCCGGTGTTTCTTGACGAAGAAATTGTTCATCAATATTACAATGGCTACTGTAACAACATCTTGTGGCCTCTTTTCCATTATCTTGGACTTCCACAAGAAGACCGCCTTGCAACCACCCGAAGTTTCCAATCTCAGTTTGATGCGTATAAGAAGGCAAACCAAATGTTTGCTGATGTTGTACATGAACACTACCAGGAGGGGGATGTCGTTTGGTGCCATGATTACCACCTAATGTTTCTTCCTAAATGCCTAAAAGAACATAACAACAAAATGAAAGTTGGTTGGTTTCTCCATACACCTTTTCCTTCCTCAGAAATACATAGGACGCTGCCATCTCGGTCAGAACTATTGAGATCGGTTCTTGCTGCCGATTTGGTTGGTTTCCACACATATGATTATGCAAGGCACTTTGTTAGTGCTTGCACTCGTATTCTTGGTTTAGAGGGTACACCTGAAGGAGTAGAGAATCAAGGAAAGCTGACTCGCGTGGCTGCGTTTCCAATTGGGATAGACTCAGACCGATTTATCCGAGCTCTAGAACTCCCTCAAGTCCAGGACCACATGAAAGAACTGAAAGAAAGATTTGCTGGAAGAAAAGTAATGTTGGGTGTTGATCGTCTCGATATGATCAAGGGGATTCCTCAAAAGATTTTGGCGTTTGAGAAATTTCTCGAGGAAAACCTGAACTGGCGTGATAAAGTAGTCTTGCTGCAAATTGCTGTACCAACAAGAACAGACGTTCCTGAGTATCAAAAGCTCACGAGTCAGGTTCATGAGATTGTCGGACGCATTAATGGGAGGTTTGGAACTCTTACTGCTGTTCCGATACATCATCTGGATCGATCTCTTGACTTTCACGCATTATGTGCACTATACGCTGTTACTGATGTAGCGCTTGTTACATCTTTAAGGGATGGAATGAATCTTGTGAGCTATGAGTTTGTTGCTTGTCAAGCTTCCAAGAAAGGAGTTCTCATTTTAAGCGAGTTTGCAGGTGCAGCACAGTCTCTTGGTGCTGGGGCTATATTAGTGAATCCTTGGAATATCACTGAAGTCGCAGCTTCCATAGGTTATGCTTTGAATATGGCAGCTGATGAAAGAGAAAAGCGTCACCATCATAACTTCATGCACGTAACAACTCACACGTCTCAAGAGTGGGCTGCAACCTTTGTAAGTGAACTCAATGATACAATTGTCGAAGCTCAACTAAGGACGAGACAAGTTCCCCCTATACTTCCAATCAAAGTAGCAGTTGATCGTTATTTGCAATCCAGCAATCGATTACTTATACTGGGATTCAATGCTACTTTAACCGAACCTAAGGACACGCTTGGGAGAAGGGGTGGCCAAATCAGAGAAATGGAACTTAAATTGCACCCAGATCTCAAGGAACCCTTGAAAAAGATTTGTGATGATTCAAAGACAACAATTGTTGTTCTCAGTGGAAGTGACAGAAGCGTCTTGGACTATAACTTCGGTGATTACAATATGTGGTTGGCAGCAGAAAATGGGATGTTCTTACGTCTTACAATGGGAGAATGGATGACAACAATGCCAGAAAATTTGAATATGGATTGGGTTGACAGCGTCAAGCATGTTTTTGAGTACTTTACTGAGAGAACACCACGGTCACATTTTGAGCTTCGTGAAACTTCACTTGTATGGAACTACAAGTATGCAGATATTGAGTTTGGAAGGCTCCAAGCTAGAGATCTGCTGCAGCATCTGTGGACAGGCCCGATCTCAAATGCATCTGTTGATGTCGTCCAAGGTGGTCGATCTGTGGAGGTCCGAGCAGTTGGTGTTACAAAGGGTGCAGCTATCGATCGTATTTTAGGAGAAATAGTTCATAACAAAGGCATGAAAGCACCGATCGATTATGTCCTCTGCATTGGACACTTTCTACCAAAGGACGAAGATGTCTATACGTTTTTTGAACCAGAGCTTCCTGTTGAGGCACCTCCAACTGTTCCTACAAGACCTCCTTCACCCACTCCCGGCATGTCATCTGCGCCACTGCCAAGGATTTCAATGAGTAAAAGTAATTCAAAGGCAGCTCGCCTTAAGAAACAACGGTCCTTGTCAACCTTAGAAAAGAGAGCTAGTATCCATGCAATCGGGAGTGCTTGGAGGCCAACGTTAATGCGTGATAAAATGTCACTCCACGAAGGTTCTTCCGTGCTTGACCTCAAGGGTGACAATTACTTCTCATGCGCTGTTGGACGAAAGAGATCAAGTGCACGATATCTCCTTGGATCGTCAGACGACGTTGTCACACTCTTGAAAGAGCTGGCTGAAGGTTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003825 GO:0005975 GO:0005984 GO:0005991 GO:0005992 GO:0006793 GO:0006796 GO:0006950 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009311 GO:0009312 GO:0009743 GO:0009756 GO:0009790 GO:0009791 GO:0009793 GO:0009832 GO:0009987 GO:0010033 GO:0010154 GO:0010182 GO:0016051 GO:0016311 GO:0016740 GO:0016757 GO:0016758 GO:0016787 GO:0016788 GO:0016791 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0033554 GO:0034637 GO:0035251 GO:0042221 GO:0042546 GO:0042578 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044262 GO:0046351 GO:0046527 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051301 GO:0051716 GO:0061458 GO:0065007 GO:0070413 GO:0070887 GO:0071310 GO:0071322 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00500 ko01100 map00500 map01100

Pfam Domains

Protein Families

Glyco_transf_20 Trehalose_PPase

Protein Analysis

930

Amino Acids

104.6

Weight (kDa)

6.77

Isoelectric Point (pI)

47.12

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001314)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G78580 AT1G78580 AT1G78580 AT1G78580
fragaria_vesca	FvH4_3g06590 FvH4_3g06590 FvH4_3g17650 FvH4_3g17650
malus_domestica	MD03G1250300.v1.1 MD10G1270400.v1.1 MD11G1270400.v1.1
prunus_persica	Prupe.4G071400_v2.0.a1 Prupe.4G071400_v2.0.a1 Prupe.4G071400_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1 Prupe.4G155900_v2.0.a1
pyrus_communis	pycom03g19800 pycom10g22630 pycom11g23920
rosa_chinensis	RchiOBHm_Chr5g0012561 RchiOBHm_Chr5g0029381
rosa_laevigata	RLG00000031925 RLG00000033160
rosa_multiflora	Rmu_sc0003577.1_g000012 Rmu_sc0004373.1_g000021
rosa_roxburghii	Rroxscaffold_1G00050580 Rroxscaffold_1G00064100
rosa_rugosa	Rorug05G0002400 Rorug05G0002400 Rorug05G0002500 Rorug05G0002600 Rorug05G0114000 Rorug05G0114000 Rorug05G0114000 Rorug05G0114000 Rorug05G0114000
rosa_samantha	Rh5AG096400 Rh5AG205600 Rh5BG092600 Rh5BG204200 Rh5CG105500 Rh5DG092100 Rh5DG207800
rosa_wichuraiana	Rw5G008440 Rw5G018730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	18
AarI	CACCTGC	1 cut(s)	1466
AasI	GACNNNNNNGTC	3 cut(s)	1940, 2402, 2753
Acc36I	ACCTGC	1 cut(s)	1466
AccB1I	GGYRCC	2 cut(s)	696, 2440
AccII	CGCG	2 cut(s)	93, 969
AccIII	TCCGGA	1 cut(s)	199
AciI	CCGC	2 cut(s)	91, 586
AclI	AACGTT	1 cut(s)	2621
AclWI	GGATC	2 cut(s)	1329, 2746
AcoI	YGGCCR	1 cut(s)	1828
AcsI	RAATTY	5 cut(s)	131, 169, 222, 1148, 2044
AcuI	CTGAAG	2 cut(s)	957, 1554
AfaI	GTAC	5 cut(s)	426, 663, 931, 1206, 2090
AfeI	AGCGCT	1 cut(s)	1377
AfiI	CCNNNNNNNGG	2 cut(s)	682, 2480
AflII	CTTAAG	1 cut(s)	2546
AflIII	ACRYGT	1 cut(s)	1626
AgsI	TTSAA	9 cut(s)	1564, 1783, 1882, 1902, 2050, 2420, 2514, 2532, 2767
AhlI	ACTAGT	1 cut(s)	358
AjiI	CACGTC	1 cut(s)	1629
AjnI	CCWGG	4 cut(s)	4, 395, 675, 1035
AloI	GAACNNNNNNTCC	4 cut(s)	1207, 1239, 2313, 2345
Alw21I	GWGCWC	4 cut(s)	370, 1018, 1354, 2725
Alw26I	GTCTC	5 cut(s)	307, 1106, 1494, 1635, 1693
Alw44I	GTGCAC	3 cut(s)	366, 1350, 2721
AlwI	GGATC	2 cut(s)	1329, 2746
AlwNI	CAGNNNCTG	1 cut(s)	2209
Ama87I	CYCGRG	1 cut(s)	1154
Aor13HI	TCCGGA	1 cut(s)	199
Aor51HI	AGCGCT	1 cut(s)	1377
AoxI	GGCC	5 cut(s)	257, 548, 1828, 2218, 2615
ApaLI	GTGCAC	3 cut(s)	366, 1350, 2721
ApoI	RAATTY	5 cut(s)	131, 169, 222, 1148, 2044
ArsI	GACNNNNNNTTYG	2 cut(s)	1940, 1972
AseI	ATTAAT	1 cut(s)	1275
Asp700I	GAANNNNTTC	3 cut(s)	57, 507, 2328
AspLEI	GCGC	3 cut(s)	1378, 2497, 2699
AspS9I	GGNCC	4 cut(s)	1039, 2219, 2272, 2559
AsuC2I	CCSGG	2 cut(s)	485, 2481
AsuHPI	GGTGA	6 cut(s)	148, 176, 1586, 1973, 2463, 2690
AvaI	CYCGRG	1 cut(s)	1154
AvaII	GGWCC	3 cut(s)	1039, 2272, 2559
AxyI	CCTNAGG	1 cut(s)	1803
BaeGI	GKGCMC	3 cut(s)	370, 1354, 2725
BaeI	ACNNNNGTAYC	2 cut(s)	1662, 1695
BalI	TGGCCA	1 cut(s)	1830
BanI	GGYRCC	2 cut(s)	696, 2440
BanII	GRGCYC	1 cut(s)	1018
BauI	CACGAG	1 cut(s)	1243
BbsI	GAAGAC	1 cut(s)	588
Bbv12I	GWGCWC	4 cut(s)	370, 1018, 1354, 2725
BccI	CCATC	5 cut(s)	107, 227, 823, 1391, 1605
BceAI	ACGGC	1 cut(s)	272
BciT130I	CCWGG	4 cut(s)	6, 397, 677, 1037
BciVI	GTATCC	1 cut(s)	2600
BclI	TGATCA	1 cut(s)	1110
BcnI	CCSGG	2 cut(s)	485, 2481
BcoDI	GTCTC	5 cut(s)	307, 1106, 1494, 1635, 1693
BcuI	ACTAGT	1 cut(s)	358
BfaI	CTAG	4 cut(s)	359, 1019, 2190, 2586
BfmI	CTRYAG	2 cut(s)	306, 2201
BfoI	RGCGCY	1 cut(s)	1379
BfrI	CTTAAG	1 cut(s)	2546
BfuAI	ACCTGC	1 cut(s)	1466
BfuI	GTATCC	1 cut(s)	2600
BglII	AGATCT	2 cut(s)	1864, 2194
BmcAI	AGTACT	1 cut(s)	2090
Bme1390I	CCNGG	6 cut(s)	6, 397, 485, 677, 1037, 2481
Bme18I	GGWCC	3 cut(s)	1039, 2272, 2559
BmeT110I	CYCGRG	1 cut(s)	1154
BmgBI	CACGTC	1 cut(s)	1629
BmgT120I	GGNCC	4 cut(s)	1039, 2219, 2272, 2559
BmiI	GGNNCC	4 cut(s)	698, 1875, 2183, 2442
BmrFI	CCNGG	6 cut(s)	6, 397, 485, 677, 1037, 2481
BmrI	ACTGGG	1 cut(s)	1784
BmsI	GCATC	6 cut(s)	136, 382, 489, 613, 2215, 2243
BmuI	ACTGGG	1 cut(s)	1784
BpiI	GAAGAC	1 cut(s)	588
Bpu10I	CCTNAGC	1 cut(s)	77
BpuEI	CTTGAG	5 cut(s)	359, 1014, 1617, 1853, 2657
BpuMI	CCSGG	2 cut(s)	485, 2481
Bsa29I	ATCGAT	4 cut(s)	1324, 1762, 2307, 2358
BsaAI	YACGTR	1 cut(s)	1615
BsaJI	CCNNGG	6 cut(s)	159, 1520, 2108, 2251, 2504, 2734
BsaWI	WCCGGW	1 cut(s)	199
Bsc4I	CCNNNNNNNGG	2 cut(s)	682, 2480
Bse1I	ACTGG	4 cut(s)	183, 282, 1175, 1779
Bse21I	CCTNAGG	1 cut(s)	1803
BseAI	TCCGGA	1 cut(s)	199
BseBI	CCWGG	4 cut(s)	6, 397, 677, 1037
BseCI	ATCGAT	4 cut(s)	1324, 1762, 2307, 2358
BseDI	CCNNGG	6 cut(s)	159, 1520, 2108, 2251, 2504, 2734
BseGI	GGATG	6 cut(s)	242, 480, 691, 1402, 2001, 2031
BseLI	CCNNNNNNNGG	2 cut(s)	682, 2480
BseMII	CTCAG	7 cut(s)	76, 629, 807, 1011, 1218, 1936, 2088
BseNI	ACTGG	4 cut(s)	183, 282, 1175, 1779
BseSI	GKGCMC	3 cut(s)	370, 1354, 2725
BseYI	CCCAGC	1 cut(s)	1499
BsgI	GTGCAG	2 cut(s)	1500, 2319
Bsh1236I	CGCG	2 cut(s)	93, 969
Bsh1285I	CGRYCG	2 cut(s)	2311, 2358
BshFI	GGCC	5 cut(s)	259, 550, 1830, 2220, 2617
BshNI	GGYRCC	2 cut(s)	696, 2440
BshVI	ATCGAT	4 cut(s)	1324, 1762, 2307, 2358
BsiEI	CGRYCG	2 cut(s)	2311, 2358
BsiHKAI	GWGCWC	4 cut(s)	370, 1018, 1354, 2725
BsiHKCI	CYCGRG	1 cut(s)	1154
BsiSI	CCGG	3 cut(s)	200, 485, 2481
BslFI	GGGAC	1 cut(s)	267
BslI	CCNNNNNNNGG	2 cut(s)	682, 2480
BsmAI	GTCTC	5 cut(s)	307, 1106, 1494, 1635, 1693
BsmBI	CGTCTC	2 cut(s)	1106, 1635
BsmFI	GGGAC	1 cut(s)	267
BsnI	GGCC	5 cut(s)	259, 550, 1830, 2220, 2617
BsoBI	CYCGRG	1 cut(s)	1154
Bsp1286I	GDGCHC	4 cut(s)	370, 1018, 1354, 2725
Bsp13I	TCCGGA	1 cut(s)	199
Bsp1407I	TGTACA	1 cut(s)	661
BspACI	CCGC	2 cut(s)	91, 586
BspANI	GGCC	5 cut(s)	259, 550, 1830, 2220, 2617
BspCNI	CTCAG	7 cut(s)	75, 628, 806, 1010, 1219, 1935, 2089
BspDI	ATCGAT	4 cut(s)	1324, 1762, 2307, 2358
BspEI	TCCGGA	1 cut(s)	199
BspFNI	CGCG	2 cut(s)	93, 969
BspHI	TCATGA	2 cut(s)	1255, 2786
BspLI	GGNNCC	4 cut(s)	698, 1875, 2183, 2442
BspMAI	CTGCAG	2 cut(s)	310, 2205
BspMI	ACCTGC	1 cut(s)	1466
BspPI	GGATC	2 cut(s)	1329, 2746
BspT107I	GGYRCC	2 cut(s)	696, 2440
BspTI	CTTAAG	1 cut(s)	2546
BsrGI	TGTACA	1 cut(s)	661
BsrI	ACTGG	4 cut(s)	183, 282, 1175, 1779
BssECI	CCNNGG	6 cut(s)	159, 1520, 2108, 2251, 2504, 2734
BssSI	CACGAG	1 cut(s)	1243
BssT1I	CCWWGG	4 cut(s)	1520, 2251, 2504, 2734
Bst2BI	CACGAG	1 cut(s)	1243
Bst2UI	CCWGG	4 cut(s)	6, 397, 677, 1037
Bst4CI	ACNGT	5 cut(s)	533, 1488, 2112, 2452, 2559
BstAFI	CTTAAG	1 cut(s)	2546
BstAPI	GCANNNNNTGC	1 cut(s)	1349
BstAUI	TGTACA	1 cut(s)	661
BstBAI	YACGTR	1 cut(s)	1615
BstC8I	GCNNGC	3 cut(s)	904, 2542, 2775
BstDSI	CCRYGG	1 cut(s)	2108
BstEII	GGTNACC	1 cut(s)	294
BstF5I	GGATG	6 cut(s)	242, 480, 691, 1402, 2001, 2031
BstFNI	CGCG	2 cut(s)	93, 969
BstH2I	RGCGCY	1 cut(s)	1379
BstHHI	GCGC	3 cut(s)	1378, 2497, 2699
BstMAI	GTCTC	5 cut(s)	307, 1106, 1494, 1635, 1693
BstMCI	CGRYCG	2 cut(s)	2311, 2358
BstMWI	GCNNNNNNNGC	4 cut(s)	1349, 1358, 1436, 1481
BstNI	CCWGG	4 cut(s)	6, 397, 677, 1037
BstNSI	RCATGY	2 cut(s)	2081, 2488
BstPI	GGTNACC	1 cut(s)	294
BstSCI	CCNGG	6 cut(s)	4, 395, 483, 675, 1035, 2479
BstSFI	CTRYAG	2 cut(s)	306, 2201
BstSLI	GKGCMC	3 cut(s)	370, 1354, 2725
BstUI	CGCG	2 cut(s)	93, 969
BstV2I	GAAGAC	1 cut(s)	588
BstX2I	RGATCY	2 cut(s)	1864, 2194
BstXI	CCANNNNNNTGG	1 cut(s)	566
BstYI	RGATCY	2 cut(s)	1864, 2194
Bsu15I	ATCGAT	4 cut(s)	1324, 1762, 2307, 2358
Bsu36I	CCTNAGG	1 cut(s)	1803
BsuI	GTATCC	1 cut(s)	2600
BsuRI	GGCC	5 cut(s)	259, 550, 1830, 2220, 2617
BsuTUI	ATCGAT	4 cut(s)	1324, 1762, 2307, 2358
BtgI	CCRYGG	1 cut(s)	2108
BtrI	CACGTC	1 cut(s)	1629
BtsCI	GGATG	6 cut(s)	242, 480, 691, 1402, 2001, 2031
BtsI	GCAGTG	1 cut(s)	2498
BtsIMutI	CAGTG	5 cut(s)	190, 370, 1530, 1930, 2498
BveI	ACCTGC	1 cut(s)	1466
Cac8I	GCNNGC	3 cut(s)	904, 2542, 2775
CaiI	CAGNNNCTG	1 cut(s)	2209
CciI	TCATGA	2 cut(s)	1255, 2786
CfoI	GCGC	3 cut(s)	1378, 2497, 2699
Cfr13I	GGNCC	4 cut(s)	1039, 2219, 2272, 2559
ClaI	ATCGAT	4 cut(s)	1324, 1762, 2307, 2358
CseI	GACGC	5 cut(s)	817, 1278, 1580, 1930, 2059
Csp6I	GTAC	5 cut(s)	425, 662, 930, 1205, 2089
CviQI	GTAC	5 cut(s)	425, 662, 930, 1205, 2089
DrdI	GACNNNNNNGTC	3 cut(s)	1940, 2402, 2753
DseDI	GACNNNNNNGTC	3 cut(s)	1940, 2402, 2753
EaeI	YGGCCR	1 cut(s)	1828
Ecl136II	GAGCTC	1 cut(s)	1016
Eco130I	CCWWGG	4 cut(s)	1520, 2251, 2504, 2734
Eco24I	GRGCYC	1 cut(s)	1018
Eco32I	GATATC	1 cut(s)	2729
Eco47I	GGWCC	3 cut(s)	1039, 2272, 2559
Eco47III	AGCGCT	1 cut(s)	1377
Eco53kI	GAGCTC	1 cut(s)	1016
Eco57I	CTGAAG	2 cut(s)	957, 1554
Eco81I	CCTNAGG	1 cut(s)	1803
Eco88I	CYCGRG	1 cut(s)	1154
Eco91I	GGTNACC	1 cut(s)	294
EcoICRI	GAGCTC	1 cut(s)	1016
EcoO65I	GGTNACC	1 cut(s)	294
EcoRI	GAATTC	1 cut(s)	131
EcoRII	CCWGG	4 cut(s)	4, 395, 675, 1035
EcoRV	GATATC	1 cut(s)	2729
EcoT14I	CCWWGG	4 cut(s)	1520, 2251, 2504, 2734
EcoT22I	ATGCAT	2 cut(s)	151, 2236
EcoT38I	GRGCYC	1 cut(s)	1018
ErhI	CCWWGG	4 cut(s)	1520, 2251, 2504, 2734
Esp3I	CGTCTC	2 cut(s)	1106, 1635
FalI	AAGNNNNNCTT	6 cut(s)	573, 605, 1862, 1894, 2452, 2484
FaqI	GGGAC	1 cut(s)	267
FauNDI	CATATG	1 cut(s)	874
FbaI	TGATCA	1 cut(s)	1110
FokI	GGATG	6 cut(s)	249, 467, 698, 1409, 2008, 2038
FriOI	GRGCYC	1 cut(s)	1018
FspBI	CTAG	4 cut(s)	359, 1019, 2190, 2586
GlaI	GCGC	3 cut(s)	1377, 2496, 2698
GsaI	CCCAGC	1 cut(s)	1503
HaeII	RGCGCY	1 cut(s)	1379
HaeIII	GGCC	5 cut(s)	259, 550, 1830, 2220, 2617
HapII	CCGG	3 cut(s)	200, 485, 2481
HgaI	GACGC	5 cut(s)	817, 1278, 1580, 1930, 2059
HhaI	GCGC	3 cut(s)	1378, 2497, 2699
Hin6I	GCGC	3 cut(s)	1376, 2495, 2697
HinP1I	GCGC	3 cut(s)	1376, 2495, 2697
HincII	GTYRAC	2 cut(s)	2065, 2568
HindII	GTYRAC	2 cut(s)	2065, 2568
HindIII	AAGCTT	2 cut(s)	459, 1437
HpaII	CCGG	3 cut(s)	200, 485, 2481
HphI	GGTGA	6 cut(s)	148, 176, 1586, 1973, 2463, 2690
Hpy166II	GTNNAC	9 cut(s)	275, 368, 932, 1352, 1661, 2065, 2214, 2568, 2723
Hpy8I	GTNNAC	9 cut(s)	275, 368, 932, 1352, 1661, 2065, 2214, 2568, 2723
Hpy99I	CGWCG	1 cut(s)	2753
HpyCH4III	ACNGT	5 cut(s)	533, 1488, 2112, 2452, 2559
HpyCH4IV	ACGT	7 cut(s)	1221, 1614, 1628, 2005, 2411, 2621, 2751
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1349, 1358, 1436, 1481
HpySE526I	ACGT	7 cut(s)	1221, 1614, 1628, 2005, 2411, 2621, 2751
HspAI	GCGC	3 cut(s)	1376, 2495, 2697
Kpn2I	TCCGGA	1 cut(s)	199
Ksp22I	TGATCA	1 cut(s)	1110
LmnI	GCTCC	1 cut(s)	2187
LweI	GCATC	6 cut(s)	136, 382, 489, 613, 2215, 2243
MaeI	CTAG	4 cut(s)	359, 1019, 2190, 2586
MaeII	ACGT	7 cut(s)	1221, 1614, 1628, 2005, 2411, 2621, 2751
MboII	GAAGA	7 cut(s)	122, 512, 593, 713, 1086, 2411, 2649
MfeI	CAATTG	3 cut(s)	984, 1674, 1911
MflI	RGATCY	2 cut(s)	1864, 2194
MhlI	GDGCHC	4 cut(s)	370, 1018, 1354, 2725
MlsI	TGGCCA	1 cut(s)	1830
MluNI	TGGCCA	1 cut(s)	1830
MlyI	GAGTC	4 cut(s)	320, 958, 989, 1255
MmeI	TCCRAC	5 cut(s)	331, 418, 1246, 2471, 2683
Mox20I	TGGCCA	1 cut(s)	1830
Mph1103I	ATGCAT	2 cut(s)	151, 2236
MroI	TCCGGA	1 cut(s)	199
MroXI	GAANNNNTTC	3 cut(s)	57, 507, 2328
MscI	TGGCCA	1 cut(s)	1830
MseI	TTAA	7 cut(s)	1275, 1391, 1463, 1793, 1851, 2547, 2624
MslI	CAYNNNNRTG	3 cut(s)	485, 873, 2143
Msp20I	TGGCCA	1 cut(s)	1830
MspA1I	CMGCKG	1 cut(s)	1574
MspCI	CTTAAG	1 cut(s)	2546
MspI	CCGG	3 cut(s)	200, 485, 2481
MspR9I	CCNGG	6 cut(s)	6, 397, 485, 677, 1037, 2481
MunI	CAATTG	3 cut(s)	984, 1674, 1911
MvaI	CCWGG	4 cut(s)	6, 397, 677, 1037
MvnI	CGCG	2 cut(s)	93, 969
MwoI	GCNNNNNNNGC	4 cut(s)	1349, 1358, 1436, 1481
NciI	CCSGG	2 cut(s)	485, 2481
NdeI	CATATG	1 cut(s)	874
NlaIV	GGNNCC	4 cut(s)	698, 1875, 2183, 2442
NmuCI	GTSAC	6 cut(s)	1592, 1931, 2112, 2640, 2678, 2755
NsiI	ATGCAT	2 cut(s)	151, 2236
NspI	RCATGY	2 cut(s)	2081, 2488
PaeR7I	CTCGAG	1 cut(s)	1154
PagI	TCATGA	2 cut(s)	1255, 2786
PaqCI	CACCTGC	1 cut(s)	1466
PdmI	GAANNNNTTC	3 cut(s)	57, 507, 2328
PfeI	GAWTC	6 cut(s)	949, 1120, 1405, 1516, 1779, 1898
PflFI	GACNNNGTC	2 cut(s)	2069, 2753
PfoI	TCCNGGA	1 cut(s)	1035
Ple19I	CGATCG	2 cut(s)	2311, 2358
PleI	GAGTC	4 cut(s)	320, 958, 989, 1254
PpsI	GAGTC	4 cut(s)	320, 958, 989, 1254
Ppu21I	YACGTR	1 cut(s)	1615
PshBI	ATTAAT	1 cut(s)	1275
PsiI	TTATAA	1 cut(s)	18
Psp124BI	GAGCTC	1 cut(s)	1018
Psp1406I	AACGTT	1 cut(s)	2621
Psp6I	CCWGG	4 cut(s)	4, 395, 675, 1035
PspEI	GGTNACC	1 cut(s)	294
PspFI	CCCAGC	1 cut(s)	1499
PspGI	CCWGG	4 cut(s)	4, 395, 675, 1035
PspN4I	GGNNCC	4 cut(s)	698, 1875, 2183, 2442
PspPI	GGNCC	4 cut(s)	1039, 2219, 2272, 2559
PstI	CTGCAG	2 cut(s)	310, 2205
PstNI	CAGNNNCTG	1 cut(s)	2209
PsuI	RGATCY	2 cut(s)	1864, 2194
PsyI	GACNNNGTC	2 cut(s)	2069, 2753
PvuI	CGATCG	2 cut(s)	2311, 2358
PvuII	CAGCTG	1 cut(s)	1574
RsaI	GTAC	5 cut(s)	426, 663, 931, 1206, 2090
RsaNI	GTAC	5 cut(s)	425, 662, 930, 1205, 2089
RseI	CAYNNNNRTG	3 cut(s)	485, 873, 2143
SacI	GAGCTC	1 cut(s)	1018
SaqAI	TTAA	7 cut(s)	1275, 1391, 1463, 1793, 1851, 2547, 2624
Sau96I	GGNCC	4 cut(s)	1039, 2219, 2272, 2559
ScaI	AGTACT	1 cut(s)	2090
SchI	GAGTC	4 cut(s)	320, 958, 989, 1255
ScrFI	CCNGG	6 cut(s)	6, 397, 485, 677, 1037, 2481
SduI	GDGCHC	4 cut(s)	370, 1018, 1354, 2725
SfaNI	GCATC	6 cut(s)	136, 382, 489, 613, 2215, 2243
SfcI	CTRYAG	2 cut(s)	306, 2201
Sfr274I	CTCGAG	1 cut(s)	1154
SinI	GGWCC	3 cut(s)	1039, 2272, 2559
SlaI	CTCGAG	1 cut(s)	1154
SmiMI	CAYNNNNRTG	3 cut(s)	485, 873, 2143
SmlI	CTYRAG	7 cut(s)	338, 1029, 1154, 1632, 1868, 2546, 2672
SmoI	CTYRAG	7 cut(s)	338, 1029, 1154, 1632, 1868, 2546, 2672
SpeI	ACTAGT	1 cut(s)	358
SsiI	CCGC	2 cut(s)	91, 586
SspI	AATATT	1 cut(s)	518
SspMI	CTAG	4 cut(s)	359, 1019, 2190, 2586
SstI	GAGCTC	1 cut(s)	1018
StyD4I	CCNGG	6 cut(s)	4, 395, 483, 675, 1035, 2479
StyI	CCWWGG	4 cut(s)	1520, 2251, 2504, 2734
TaaI	ACNGT	5 cut(s)	533, 1488, 2112, 2452, 2559
TaiI	ACGT	7 cut(s)	1224, 1617, 1631, 2008, 2414, 2624, 2754
TaqI	TCGA	9 cut(s)	390, 1104, 1155, 1324, 1680, 1762, 2260, 2307, 2358
TaqII	GACCGA	2 cut(s)	810, 1017
TatI	WGTACW	2 cut(s)	661, 2088
TfiI	GAWTC	6 cut(s)	949, 1120, 1405, 1516, 1779, 1898
Tru1I	TTAA	7 cut(s)	1275, 1391, 1463, 1793, 1851, 2547, 2624
Tru9I	TTAA	7 cut(s)	1275, 1391, 1463, 1793, 1851, 2547, 2624
TscAI	CASTG	5 cut(s)	190, 370, 1537, 1930, 2505
TseFI	GTSAC	6 cut(s)	1592, 1931, 2112, 2640, 2678, 2755
Tsp45I	GTSAC	6 cut(s)	1592, 1931, 2112, 2640, 2678, 2755
TspGWI	ACGGA	1 cut(s)	2650
TspRI	CASTG	5 cut(s)	190, 370, 1537, 1930, 2505
Tth111I	GACNNNGTC	2 cut(s)	2069, 2753
Vha464I	CTTAAG	1 cut(s)	2546
VneI	GTGCAC	3 cut(s)	366, 1350, 2721
VpaK11BI	GGWCC	3 cut(s)	1039, 2272, 2559
VspI	ATTAAT	1 cut(s)	1275
XapI	RAATTY	5 cut(s)	131, 169, 222, 1148, 2044
XbaI	TCTAGA	1 cut(s)	1018
XceI	RCATGY	2 cut(s)	2081, 2488
XhoI	CTCGAG	1 cut(s)	1154
XmnI	GAANNNNTTC	3 cut(s)	57, 507, 2328
XspI	CTAG	4 cut(s)	359, 1019, 2190, 2586
ZrmI	AGTACT	1 cut(s)	2090
Zsp2I	ATGCAT	2 cut(s)	151, 2236

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.