alpha,alpha-trehalose-phosphate synthase UDP-forming
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 1705 |
| AccB1I | GGYRCC | 4 cut(s) | 696, 1478, 1496, 1705 |
| AciI | CCGC | 1 cut(s) | 1482 |
| AclI | AACGTT | 1 cut(s) | 1741 |
| AclWI | GGATC | 5 cut(s) | 957, 1329, 1892, 1905, 2432 |
| AcsI | RAATTY | 3 cut(s) | 177, 798, 1334 |
| AcuI | CTGAAG | 5 cut(s) | 957, 1596, 2708, 2760, 2774 |
| AdeI | CACNNNGTG | 1 cut(s) | 2501 |
| AfaI | GTAC | 8 cut(s) | 17, 931, 1707, 1967, 2379, 2420, 2652, 2704 |
| AfeI | AGCGCT | 1 cut(s) | 1887 |
| AflIII | ACRYGT | 2 cut(s) | 1612, 1983 |
| AjnI | CCWGG | 4 cut(s) | 4, 1317, 1632, 2497 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 1207, 1239, 2325, 2357 |
| Alw21I | GWGCWC | 3 cut(s) | 831, 1018, 1354 |
| Alw26I | GTCTC | 6 cut(s) | 307, 1693, 1724, 1813, 2608, 2749 |
| Alw44I | GTGCAC | 1 cut(s) | 1350 |
| AlwI | GGATC | 5 cut(s) | 957, 1329, 1892, 1905, 2432 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 274 |
| Ama87I | CYCGRG | 1 cut(s) | 966 |
| Aor51HI | AGCGCT | 1 cut(s) | 1887 |
| AoxI | GGCC | 4 cut(s) | 222, 257, 548, 2621 |
| ApaI | GGGCCC | 1 cut(s) | 226 |
| ApaLI | GTGCAC | 1 cut(s) | 1350 |
| ApoI | RAATTY | 3 cut(s) | 177, 798, 1334 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 744, 776 |
| AseI | ATTAAT | 1 cut(s) | 1275 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 994, 1149, 2340 |
| Asp718I | GGTACC | 1 cut(s) | 1705 |
| AspLEI | GCGC | 3 cut(s) | 837, 1687, 1888 |
| AspS9I | GGNCC | 6 cut(s) | 222, 223, 549, 1453, 2231, 2621 |
| AsuC2I | CCSGG | 1 cut(s) | 2122 |
| AsuHPI | GGTGA | 6 cut(s) | 145, 332, 695, 1307, 2487, 2513 |
| AsuII | TTCGAA | 2 cut(s) | 2060, 2133 |
| AvaI | CYCGRG | 1 cut(s) | 966 |
| AvaII | GGWCC | 2 cut(s) | 1453, 2231 |
| AxyI | CCTNAGG | 1 cut(s) | 1391 |
| BaeGI | GKGCMC | 2 cut(s) | 226, 1354 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 1124, 1157, 1662, 1695 |
| BamHI | GGATCC | 1 cut(s) | 1897 |
| BanI | GGYRCC | 4 cut(s) | 696, 1478, 1496, 1705 |
| BanII | GRGCYC | 2 cut(s) | 226, 831 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2657, 2689 |
| BauI | CACGAG | 1 cut(s) | 2153 |
| BbsI | GAAGAC | 3 cut(s) | 481, 2197, 2453 |
| Bbv12I | GWGCWC | 3 cut(s) | 831, 1018, 1354 |
| BccI | CCATC | 6 cut(s) | 151, 402, 717, 823, 1391, 2028 |
| BceAI | ACGGC | 2 cut(s) | 71, 1314 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1322, 1356 |
| BciT130I | CCWGG | 4 cut(s) | 6, 1319, 1634, 2499 |
| BciVI | GTATCC | 1 cut(s) | 491 |
| BcnI | CCSGG | 1 cut(s) | 2122 |
| BcoDI | GTCTC | 6 cut(s) | 307, 1693, 1724, 1813, 2608, 2749 |
| BfaI | CTAG | 3 cut(s) | 464, 1511, 2654 |
| BfmI | CTRYAG | 1 cut(s) | 210 |
| BfoI | RGCGCY | 1 cut(s) | 1889 |
| BfuI | GTATCC | 1 cut(s) | 491 |
| BglII | AGATCT | 1 cut(s) | 2510 |
| Bme1390I | CCNGG | 5 cut(s) | 6, 1319, 1634, 2122, 2499 |
| Bme18I | GGWCC | 2 cut(s) | 1453, 2231 |
| BmeT110I | CYCGRG | 1 cut(s) | 966 |
| BmgT120I | GGNCC | 6 cut(s) | 222, 223, 549, 1453, 2231, 2621 |
| BmrFI | CCNGG | 5 cut(s) | 6, 1319, 1634, 2122, 2499 |
| BmrI | ACTGGG | 1 cut(s) | 1784 |
| BmsI | GCATC | 7 cut(s) | 104, 1429, 1867, 2227, 2255, 2758, 2780 |
| BmuI | ACTGGG | 1 cut(s) | 1784 |
| BoxI | GACNNNNGTC | 2 cut(s) | 1301, 2491 |
| BpiI | GAAGAC | 3 cut(s) | 481, 2197, 2453 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 155, 187, 949, 981 |
| BpmI | CTGGAG | 1 cut(s) | 2613 |
| Bpu14I | TTCGAA | 2 cut(s) | 2060, 2133 |
| BpuEI | CTTGAG | 7 cut(s) | 82, 157, 944, 1014, 1040, 1175, 2645 |
| BpuMI | CCSGG | 1 cut(s) | 2122 |
| Bsa29I | ATCGAT | 1 cut(s) | 1762 |
| BsaAI | YACGTR | 1 cut(s) | 1747 |
| BsaBI | GATNNNNATC | 1 cut(s) | 999 |
| BsaJI | CCNNGG | 5 cut(s) | 5, 565, 1520, 1632, 2120 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 2325, 2355, 2686, 2716 |
| Bse118I | RCCGGY | 1 cut(s) | 2271 |
| Bse1I | ACTGG | 5 cut(s) | 610, 1140, 1779, 1802, 2233 |
| Bse21I | CCTNAGG | 1 cut(s) | 1391 |
| Bse8I | GATNNNNATC | 1 cut(s) | 999 |
| BseBI | CCWGG | 4 cut(s) | 6, 1319, 1634, 2499 |
| BseCI | ATCGAT | 1 cut(s) | 1762 |
| BseDI | CCNNGG | 5 cut(s) | 5, 565, 1520, 1632, 2120 |
| BseGI | GGATG | 8 cut(s) | 413, 1385, 1402, 1858, 2039, 2043, 2360, 2413 |
| BseJI | GATNNNNATC | 1 cut(s) | 999 |
| BseMII | CTCAG | 8 cut(s) | 138, 277, 852, 1218, 1405, 1806, 1936, 2577 |
| BseNI | ACTGG | 5 cut(s) | 610, 1140, 1779, 1802, 2233 |
| BseRI | GAGGAG | 3 cut(s) | 248, 515, 1017 |
| BseSI | GKGCMC | 2 cut(s) | 226, 1354 |
| BseYI | CCCAGC | 1 cut(s) | 350 |
| BsgI | GTGCAG | 3 cut(s) | 889, 2105, 2331 |
| BshFI | GGCC | 4 cut(s) | 224, 259, 550, 2623 |
| BshNI | GGYRCC | 4 cut(s) | 696, 1478, 1496, 1705 |
| BshVI | ATCGAT | 1 cut(s) | 1762 |
| BsiHKAI | GWGCWC | 3 cut(s) | 831, 1018, 1354 |
| BsiHKCI | CYCGRG | 1 cut(s) | 966 |
| BsiSI | CCGG | 3 cut(s) | 1500, 2122, 2272 |
| BsiWI | CGTACG | 1 cut(s) | 2702 |
| BslFI | GGGAC | 2 cut(s) | 1289, 1555 |
| BsmAI | GTCTC | 6 cut(s) | 307, 1693, 1724, 1813, 2608, 2749 |
| BsmBI | CGTCTC | 2 cut(s) | 307, 1813 |
| BsmFI | GGGAC | 2 cut(s) | 1289, 1555 |
| BsmI | GAATGC | 2 cut(s) | 912, 2713 |
| BsnI | GGCC | 4 cut(s) | 224, 259, 550, 2623 |
| BsoBI | CYCGRG | 1 cut(s) | 966 |
| Bsp119I | TTCGAA | 2 cut(s) | 2060, 2133 |
| Bsp120I | GGGCCC | 1 cut(s) | 222 |
| Bsp1286I | GDGCHC | 4 cut(s) | 226, 831, 1018, 1354 |
| Bsp19I | CCATGG | 1 cut(s) | 1520 |
| BspACI | CCGC | 1 cut(s) | 1482 |
| BspANI | GGCC | 4 cut(s) | 224, 259, 550, 2623 |
| BspCNI | CTCAG | 8 cut(s) | 139, 276, 851, 1219, 1404, 1807, 1935, 2578 |
| BspDI | ATCGAT | 1 cut(s) | 1762 |
| BspHI | TCATGA | 1 cut(s) | 1255 |
| BspMAI | CTGCAG | 1 cut(s) | 214 |
| BspPI | GGATC | 5 cut(s) | 957, 1329, 1892, 1905, 2432 |
| BspT104I | TTCGAA | 2 cut(s) | 2060, 2133 |
| BspT107I | GGYRCC | 4 cut(s) | 696, 1478, 1496, 1705 |
| BsrFI | RCCGGY | 1 cut(s) | 2271 |
| BsrI | ACTGG | 5 cut(s) | 610, 1140, 1779, 1802, 2233 |
| BssAI | RCCGGY | 1 cut(s) | 2271 |
| BssECI | CCNNGG | 5 cut(s) | 5, 565, 1520, 1632, 2120 |
| BssSI | CACGAG | 1 cut(s) | 2153 |
| BssT1I | CCWWGG | 2 cut(s) | 565, 1520 |
| Bst2BI | CACGAG | 1 cut(s) | 2153 |
| Bst2UI | CCWGG | 4 cut(s) | 6, 1319, 1634, 2499 |
| Bst4CI | ACNGT | 7 cut(s) | 23, 1356, 1488, 1675, 1965, 2324, 2382 |
| Bst6I | CTCTTC | 3 cut(s) | 156, 672, 1572 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1358 |
| BstBAI | YACGTR | 1 cut(s) | 1747 |
| BstBI | TTCGAA | 2 cut(s) | 2060, 2133 |
| BstC8I | GCNNGC | 4 cut(s) | 904, 1432, 1752, 2289 |
| BstDSI | CCRYGG | 1 cut(s) | 1520 |
| BstEII | GGTNACC | 1 cut(s) | 294 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 230, 497, 2663 |
| BstF5I | GGATG | 8 cut(s) | 413, 1385, 1402, 1858, 2039, 2043, 2360, 2413 |
| BstH2I | RGCGCY | 1 cut(s) | 1889 |
| BstHHI | GCGC | 3 cut(s) | 837, 1687, 1888 |
| BstMAI | GTCTC | 6 cut(s) | 307, 1693, 1724, 1813, 2608, 2749 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 215, 221, 244, 1022, 1349, 1358, 1481, 1597 |
| BstNI | CCWGG | 4 cut(s) | 6, 1319, 1634, 2499 |
| BstNSI | RCATGY | 4 cut(s) | 1434, 1616, 1987, 2093 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 1301, 2491 |
| BstPI | GGTNACC | 1 cut(s) | 294 |
| BstSCI | CCNGG | 5 cut(s) | 4, 1317, 1632, 2120, 2497 |
| BstSFI | CTRYAG | 1 cut(s) | 210 |
| BstSLI | GKGCMC | 2 cut(s) | 226, 1354 |
| BstV2I | GAAGAC | 3 cut(s) | 481, 2197, 2453 |
| BstX2I | RGATCY | 3 cut(s) | 1897, 2437, 2510 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 566 |
| BstYI | RGATCY | 3 cut(s) | 1897, 2437, 2510 |
| Bsu15I | ATCGAT | 1 cut(s) | 1762 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1391 |
| BsuI | GTATCC | 1 cut(s) | 491 |
| BsuRI | GGCC | 4 cut(s) | 224, 259, 550, 2623 |
| BsuTUI | ATCGAT | 1 cut(s) | 1762 |
| BtgI | CCRYGG | 1 cut(s) | 1520 |
| BtgZI | GCGATG | 1 cut(s) | 2535 |
| BtsCI | GGATG | 8 cut(s) | 413, 1385, 1402, 1858, 2039, 2043, 2360, 2413 |
| BtsI | GCAGTG | 4 cut(s) | 363, 809, 1209, 1626 |
| BtsIMutI | CAGTG | 7 cut(s) | 363, 809, 1209, 1352, 1626, 1809, 1930 |
| Cac8I | GCNNGC | 4 cut(s) | 904, 1432, 1752, 2289 |
| CaiI | CAGNNNCTG | 1 cut(s) | 274 |
| CciI | TCATGA | 1 cut(s) | 1255 |
| CfoI | GCGC | 3 cut(s) | 837, 1687, 1888 |
| Cfr10I | RCCGGY | 1 cut(s) | 2271 |
| Cfr13I | GGNCC | 6 cut(s) | 222, 223, 549, 1453, 2231, 2621 |
| ClaI | ATCGAT | 1 cut(s) | 1762 |
| CsiI | ACCWGGT | 1 cut(s) | 2497 |
| Csp6I | GTAC | 8 cut(s) | 16, 930, 1706, 1966, 2378, 2419, 2651, 2703 |
| CviQI | GTAC | 8 cut(s) | 16, 930, 1706, 1966, 2378, 2419, 2651, 2703 |
| DraIII | CACNNNGTG | 1 cut(s) | 2501 |
| Eam1104I | CTCTTC | 3 cut(s) | 156, 672, 1572 |
| EarI | CTCTTC | 3 cut(s) | 156, 672, 1572 |
| Ecl136II | GAGCTC | 1 cut(s) | 829 |
| Eco130I | CCWWGG | 2 cut(s) | 565, 1520 |
| Eco147I | AGGCCT | 1 cut(s) | 259 |
| Eco24I | GRGCYC | 2 cut(s) | 226, 831 |
| Eco47I | GGWCC | 2 cut(s) | 1453, 2231 |
| Eco47III | AGCGCT | 1 cut(s) | 1887 |
| Eco53kI | GAGCTC | 1 cut(s) | 829 |
| Eco57I | CTGAAG | 5 cut(s) | 957, 1596, 2708, 2760, 2774 |
| Eco81I | CCTNAGG | 1 cut(s) | 1391 |
| Eco88I | CYCGRG | 1 cut(s) | 966 |
| Eco91I | GGTNACC | 1 cut(s) | 294 |
| EcoICRI | GAGCTC | 1 cut(s) | 829 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 230, 497, 2663 |
| EcoO109I | RGGNCCY | 4 cut(s) | 222, 223, 1453, 2621 |
| EcoO65I | GGTNACC | 1 cut(s) | 294 |
| EcoRII | CCWGG | 4 cut(s) | 4, 1317, 1632, 2497 |
| EcoT14I | CCWWGG | 2 cut(s) | 565, 1520 |
| EcoT22I | ATGCAT | 2 cut(s) | 2248, 2773 |
| EcoT38I | GRGCYC | 2 cut(s) | 226, 831 |
| ErhI | CCWWGG | 2 cut(s) | 565, 1520 |
| Esp3I | CGTCTC | 2 cut(s) | 307, 1813 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 573, 605, 945, 977, 1440, 1472, 2608, 2640 |
| FaqI | GGGAC | 2 cut(s) | 1289, 1555 |
| FokI | GGATG | 8 cut(s) | 420, 1372, 1409, 1845, 2046, 2050, 2347, 2420 |
| FriOI | GRGCYC | 2 cut(s) | 226, 831 |
| FspBI | CTAG | 3 cut(s) | 464, 1511, 2654 |
| GlaI | GCGC | 3 cut(s) | 836, 1686, 1887 |
| GsaI | CCCAGC | 1 cut(s) | 354 |
| GsuI | CTGGAG | 1 cut(s) | 2613 |
| HaeII | RGCGCY | 1 cut(s) | 1889 |
| HaeIII | GGCC | 4 cut(s) | 224, 259, 550, 2623 |
| HapII | CCGG | 3 cut(s) | 1500, 2122, 2272 |
| HhaI | GCGC | 3 cut(s) | 837, 1687, 1888 |
| Hin6I | GCGC | 3 cut(s) | 835, 1685, 1886 |
| HinP1I | GCGC | 3 cut(s) | 835, 1685, 1886 |
| HincII | GTYRAC | 1 cut(s) | 1794 |
| HindII | GTYRAC | 1 cut(s) | 1794 |
| HindIII | AAGCTT | 1 cut(s) | 2519 |
| HinfI | GANTC | 7 cut(s) | 136, 326, 995, 1119, 1405, 1779, 2698 |
| HpaI | GTTAAC | 1 cut(s) | 1794 |
| HpaII | CCGG | 3 cut(s) | 1500, 2122, 2272 |
| HphI | GGTGA | 6 cut(s) | 145, 332, 695, 1307, 2487, 2513 |
| Hpy166II | GTNNAC | 5 cut(s) | 1352, 1516, 1794, 1807, 2419 |
| Hpy8I | GTNNAC | 5 cut(s) | 1352, 1516, 1794, 1807, 2419 |
| Hpy99I | CGWCG | 2 cut(s) | 1792, 2142 |
| HpyAV | CCTTC | 9 cut(s) | 197, 439, 628, 730, 798, 932, 2634, 2659, 2749 |
| HpyCH4III | ACNGT | 7 cut(s) | 23, 1356, 1488, 1675, 1965, 2324, 2382 |
| HpyCH4IV | ACGT | 6 cut(s) | 1371, 1741, 1746, 1790, 2017, 2137 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 215, 221, 244, 1022, 1349, 1358, 1481, 1597 |
| HpySE526I | ACGT | 6 cut(s) | 1371, 1741, 1746, 1790, 2017, 2137 |
| HspAI | GCGC | 3 cut(s) | 835, 1685, 1886 |
| KpnI | GGTACC | 1 cut(s) | 1709 |
| KspAI | GTTAAC | 1 cut(s) | 1794 |
| LmnI | GCTCC | 5 cut(s) | 235, 834, 1030, 1502, 1975 |
| LweI | GCATC | 7 cut(s) | 104, 1429, 1867, 2227, 2255, 2758, 2780 |
| MabI | ACCWGGT | 1 cut(s) | 2497 |
| MaeI | CTAG | 3 cut(s) | 464, 1511, 2654 |
| MaeII | ACGT | 6 cut(s) | 1371, 1741, 1746, 1790, 2017, 2137 |
| MfeI | CAATTG | 3 cut(s) | 104, 1548, 2316 |
| MflI | RGATCY | 3 cut(s) | 1897, 2437, 2510 |
| MhlI | GDGCHC | 4 cut(s) | 226, 831, 1018, 1354 |
| MlyI | GAGTC | 1 cut(s) | 2692 |
| MmeI | TCCRAC | 1 cut(s) | 1246 |
| Mph1103I | ATGCAT | 2 cut(s) | 2248, 2773 |
| MroXI | GAANNNNTTC | 3 cut(s) | 994, 1149, 2340 |
| MseI | TTAA | 7 cut(s) | 228, 663, 1047, 1113, 1275, 1793, 2078 |
| MslI | CAYNNNNRTG | 1 cut(s) | 873 |
| MspA1I | CMGCKG | 3 cut(s) | 215, 851, 1997 |
| MspI | CCGG | 3 cut(s) | 1500, 2122, 2272 |
| MspR9I | CCNGG | 5 cut(s) | 6, 1319, 1634, 2122, 2499 |
| MunI | CAATTG | 3 cut(s) | 104, 1548, 2316 |
| Mva1269I | GAATGC | 2 cut(s) | 912, 2713 |
| MvaI | CCWGG | 4 cut(s) | 6, 1319, 1634, 2499 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 215, 221, 244, 1022, 1349, 1358, 1481, 1597 |
| NciI | CCSGG | 1 cut(s) | 2122 |
| NcoI | CCATGG | 1 cut(s) | 1520 |
| NmuCI | GTSAC | 8 cut(s) | 361, 1175, 1381, 1615, 1931, 2124, 2299, 2493 |
| NsiI | ATGCAT | 2 cut(s) | 2248, 2773 |
| NspI | RCATGY | 4 cut(s) | 1434, 1616, 1987, 2093 |
| NspV | TTCGAA | 2 cut(s) | 2060, 2133 |
| PaeI | GCATGC | 1 cut(s) | 1434 |
| PaeR7I | CTCGAG | 1 cut(s) | 966 |
| PagI | TCATGA | 1 cut(s) | 1255 |
| PceI | AGGCCT | 1 cut(s) | 259 |
| PciI | ACATGT | 2 cut(s) | 1612, 1983 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1009, 1747 |
| PctI | GAATGC | 2 cut(s) | 912, 2713 |
| PdmI | GAANNNNTTC | 3 cut(s) | 994, 1149, 2340 |
| PfeI | GAWTC | 6 cut(s) | 136, 326, 995, 1119, 1405, 1779 |
| Pfl23II | CGTACG | 1 cut(s) | 2702 |
| PleI | GAGTC | 1 cut(s) | 2692 |
| PpsI | GAGTC | 1 cut(s) | 2692 |
| Ppu21I | YACGTR | 1 cut(s) | 1747 |
| PpuMI | RGGWCCY | 1 cut(s) | 1453 |
| PscI | ACATGT | 2 cut(s) | 1612, 1983 |
| PshAI | GACNNNNGTC | 2 cut(s) | 1301, 2491 |
| PshBI | ATTAAT | 1 cut(s) | 1275 |
| Psp124BI | GAGCTC | 1 cut(s) | 831 |
| Psp1406I | AACGTT | 1 cut(s) | 1741 |
| Psp5II | RGGWCCY | 1 cut(s) | 1453 |
| Psp6I | CCWGG | 4 cut(s) | 4, 1317, 1632, 2497 |
| PspEI | GGTNACC | 1 cut(s) | 294 |
| PspFI | CCCAGC | 1 cut(s) | 350 |
| PspGI | CCWGG | 4 cut(s) | 4, 1317, 1632, 2497 |
| PspLI | CGTACG | 1 cut(s) | 2702 |
| PspOMI | GGGCCC | 1 cut(s) | 222 |
| PspPI | GGNCC | 6 cut(s) | 222, 223, 549, 1453, 2231, 2621 |
| PspPPI | RGGWCCY | 1 cut(s) | 1453 |
| PspXI | VCTCGAGB | 1 cut(s) | 966 |
| PstI | CTGCAG | 1 cut(s) | 214 |
| PstNI | CAGNNNCTG | 1 cut(s) | 274 |
| PsuI | RGATCY | 3 cut(s) | 1897, 2437, 2510 |
| PvuII | CAGCTG | 3 cut(s) | 215, 851, 1997 |
| RsaI | GTAC | 8 cut(s) | 17, 931, 1707, 1967, 2379, 2420, 2652, 2704 |
| RsaNI | GTAC | 8 cut(s) | 16, 930, 1706, 1966, 2378, 2419, 2651, 2703 |
| RseI | CAYNNNNRTG | 1 cut(s) | 873 |
| SacI | GAGCTC | 1 cut(s) | 831 |
| SaqAI | TTAA | 7 cut(s) | 228, 663, 1047, 1113, 1275, 1793, 2078 |
| Sau96I | GGNCC | 6 cut(s) | 222, 223, 549, 1453, 2231, 2621 |
| SchI | GAGTC | 1 cut(s) | 2692 |
| ScrFI | CCNGG | 5 cut(s) | 6, 1319, 1634, 2122, 2499 |
| SduI | GDGCHC | 4 cut(s) | 226, 831, 1018, 1354 |
| SexAI | ACCWGGT | 1 cut(s) | 2497 |
| SfaNI | GCATC | 7 cut(s) | 104, 1429, 1867, 2227, 2255, 2758, 2780 |
| SfcI | CTRYAG | 1 cut(s) | 210 |
| Sfr274I | CTCGAG | 1 cut(s) | 966 |
| SfuI | TTCGAA | 2 cut(s) | 2060, 2133 |
| SinI | GGWCC | 2 cut(s) | 1453, 2231 |
| SlaI | CTCGAG | 1 cut(s) | 966 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 873 |
| SmlI | CTYRAG | 8 cut(s) | 61, 172, 923, 966, 1019, 1029, 1154, 2660 |
| SmoI | CTYRAG | 8 cut(s) | 61, 172, 923, 966, 1019, 1029, 1154, 2660 |
| SphI | GCATGC | 1 cut(s) | 1434 |
| SseBI | AGGCCT | 1 cut(s) | 259 |
| SsiI | CCGC | 1 cut(s) | 1482 |
| SspI | AATATT | 3 cut(s) | 518, 541, 2455 |
| SspMI | CTAG | 3 cut(s) | 464, 1511, 2654 |
| SstI | GAGCTC | 1 cut(s) | 831 |
| StuI | AGGCCT | 1 cut(s) | 259 |
| StyD4I | CCNGG | 5 cut(s) | 4, 1317, 1632, 2120, 2497 |
| StyI | CCWWGG | 2 cut(s) | 565, 1520 |
| TaaI | ACNGT | 7 cut(s) | 23, 1356, 1488, 1675, 1965, 2324, 2382 |
| TaiI | ACGT | 6 cut(s) | 1374, 1744, 1749, 1793, 2020, 2140 |
| TatI | WGTACW | 4 cut(s) | 15, 2377, 2418, 2650 |
| TauI | GCSGC | 1 cut(s) | 1484 |
| TfiI | GAWTC | 6 cut(s) | 136, 326, 995, 1119, 1405, 1779 |
| Tru1I | TTAA | 7 cut(s) | 228, 663, 1047, 1113, 1275, 1793, 2078 |
| Tru9I | TTAA | 7 cut(s) | 228, 663, 1047, 1113, 1275, 1793, 2078 |
| TscAI | CASTG | 7 cut(s) | 370, 816, 1209, 1359, 1633, 1809, 1930 |
| TseFI | GTSAC | 8 cut(s) | 361, 1175, 1381, 1615, 1931, 2124, 2299, 2493 |
| Tsp45I | GTSAC | 8 cut(s) | 361, 1175, 1381, 1615, 1931, 2124, 2299, 2493 |
| TspGWI | ACGGA | 2 cut(s) | 1708, 2499 |
| TspRI | CASTG | 7 cut(s) | 370, 816, 1209, 1359, 1633, 1809, 1930 |
| VneI | GTGCAC | 1 cut(s) | 1350 |
| VpaK11BI | GGWCC | 2 cut(s) | 1453, 2231 |
| VspI | ATTAAT | 1 cut(s) | 1275 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 230, 497, 2663 |
| XapI | RAATTY | 3 cut(s) | 177, 798, 1334 |
| XceI | RCATGY | 4 cut(s) | 1434, 1616, 1987, 2093 |
| XhoI | CTCGAG | 1 cut(s) | 966 |
| XmnI | GAANNNNTTC | 3 cut(s) | 994, 1149, 2340 |
| XspI | CTAG | 3 cut(s) | 464, 1511, 2654 |
| Zsp2I | ATGCAT | 2 cut(s) | 2248, 2773 |