MD03G1265000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012509
Physical Location & Seq
Forward (+)
35046023 .. 35047691
1669 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1265000.v1.1.491

Sequence Viewer

Length: 981 bp
ATGGCAGCTTCAGCGGACACGCCATCGTCTATCCACCTCAACAAGCAGTCACTCATGGATTCTTCTTCTCCGGTATTCAGCCCCTCCTCCGATAAGCGCTTCTGGAGCTCTCTCCGCAGCCGGATTGACGCGCTTTTAGATGATCGCAGCTCCACAATCCCAATTGCTCAGAGCACACAAAATGTTGATCCGTCTCGGCCAATTCAAACGAATGGCGGAGGATCGAAACGAGCAAGAGCGACGAAGGACGACTCATTGCTTTTGATGAGAGGGTTTGATTCCATAGCTCACACTTTGTCTCAGCTCTCGAACACTTTGGATAATGCTCTTCAGAGAGAGAAGGATTCGGAAGAACAGCAGAATGCAGAAGATTCCAATGTTGGATTGAAGAGGAAATTCGACGACAGCCATTGCTCAGAGGAACAAGGTGATGAATCCCAGAAAGAACATGAGCAAGAAGTCCCGAGAGACAGGAAGCTGAAGAAAGCGAAAAATCTTGCGATTTCTATGGCAACAAAAGCAGCTTCGTTTGCCCGGGAACTCAAGTCGATAAGATTGGACTTATCCTTTATGCAAGAGCGCTGTGTTTTGCTGGAGGAGGAGAATCGGAGGCTCCGAGACGGGCGGGATAAAGGGTTGAGGCTTGAGGAAGATGATCTGGTGAGGCTTCAGTTAGAGGCACTGCTAGCCGAGAAATCAAGACTAGCAAATGAAAATTCAAACCTAGTTAGGGAAAATCAATGCCTCCACCAGCTTGTGGAGTACCACCAGCTCACCTCCCAAGACCTCTCTGATTCATATGAACAAGTTGTACAAGGGTTGTGCCTAGACTTCTCGTCTCCTCCACGACCGACAGCAGACGAGGCAAATGGTGGAGATGGTGATGCTACTGCTGATAATGAAGTTTCTCAGACACCAAGAAGTAATCTTTTAGGGTTCTCTGCTTCGCTTGATGATGAGTTTTCTCATGGAGATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

327

Amino Acids

36.33

Weight (kDa)

4.91

Isoelectric Point (pI)

55.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012509)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02800
fragaria_vesca FvH4_2g23580 FvH4_2g23580
malus_domestica MD03G1265000.v1.1 MD11G1285200.v1.1
prunus_persica Prupe.8G241100_v2.0.a1
pyrus_communis pycom03g21210 pycom11g25280 pycom11g25340
rosa_chinensis RchiOBHm_Chr6g0291051
rosa_laevigata RLG00000012151
rosa_multiflora Rmu_sc0004642.1_g000003
rosa_roxburghii Rroxscaffold_7G00176420
rosa_rugosa Rorug06G0217200
rosa_samantha Rh6AG327300 Rh6BG334800 Rh6CG341000 Rh6DG328300
rosa_wichuraiana Rw6G028470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 757
AccII CGCG 1 cut(s) 131
AciI CCGC 4 cut(s) 14, 115, 216, 625
AclWI GGATC 2 cut(s) 182, 229
AcoI YGGCCR 1 cut(s) 197
AcsI RAATTY 2 cut(s) 395, 715
AcuI CTGAAG 3 cut(s) 314, 500, 653
AfaI GTAC 2 cut(s) 764, 813
AfeI AGCGCT 2 cut(s) 98, 581
AfiI CCNNNNNNNGG 2 cut(s) 730, 757
AgsI TTSAA 3 cut(s) 206, 388, 720
AhdI GACNNNNNGTC 1 cut(s) 835
AluBI AGCT 9 cut(s) 8, 108, 150, 287, 304, 478, 524, 754, 772
AluI AGCT 9 cut(s) 8, 108, 150, 287, 304, 478, 524, 754, 772
Alw21I GWGCWC 2 cut(s) 110, 176
Alw26I GTCTC 5 cut(s) 198, 303, 462, 612, 843
AlwI GGATC 2 cut(s) 182, 229
Ama87I CYCGRG 2 cut(s) 463, 534
Aor51HI AGCGCT 2 cut(s) 98, 581
AoxI GGCC 1 cut(s) 197
ApeKI GCWGC 4 cut(s) 5, 117, 147, 521
ApoI RAATTY 2 cut(s) 395, 715
AspLEI GCGC 3 cut(s) 99, 133, 582
AsuC2I CCSGG 2 cut(s) 535, 536
AsuHPI GGTGA 4 cut(s) 440, 673, 766, 893
AsuNHI GCTAGC 1 cut(s) 685
AvaI CYCGRG 2 cut(s) 463, 534
BanII GRGCYC 1 cut(s) 110
Bbv12I GWGCWC 2 cut(s) 110, 176
BbvI GCAGC 4 cut(s) 17, 129, 159, 533
BccI CCATC 2 cut(s) 31, 872
BcnI CCSGG 2 cut(s) 535, 536
BcoDI GTCTC 5 cut(s) 198, 303, 462, 612, 843
BfaI CTAG 4 cut(s) 686, 704, 725, 827
BfoI RGCGCY 2 cut(s) 100, 583
BisI GCNGC 4 cut(s) 6, 118, 148, 522
BlsI GCNGC 4 cut(s) 7, 119, 149, 523
Bme1390I CCNGG 2 cut(s) 535, 536
BmeRI GACNNNNNGTC 1 cut(s) 835
BmeT110I CYCGRG 2 cut(s) 463, 534
BmiI GGNNCC 1 cut(s) 614
BmrFI CCNGG 2 cut(s) 535, 536
BmsI GCATC 1 cut(s) 874
BmtI GCTAGC 1 cut(s) 689
BpmI CTGGAG 2 cut(s) 124, 614
BpuEI CTTGAG 2 cut(s) 527, 665
BpuMI CCSGG 2 cut(s) 535, 536
BsaJI CCNNGG 1 cut(s) 534
BsaWI WCCGGW 1 cut(s) 70
Bsc4I CCNNNNNNNGG 2 cut(s) 730, 757
Bse3DI GCAATG 2 cut(s) 254, 409
BseDI CCNNGG 1 cut(s) 534
BseLI CCNNNNNNNGG 2 cut(s) 730, 757
BseMI GCAATG 2 cut(s) 254, 409
BseMII CTCAG 4 cut(s) 182, 314, 429, 923
BseRI GAGGAG 4 cut(s) 76, 611, 614, 831
BseXI GCAGC 4 cut(s) 17, 129, 159, 533
Bsh1236I CGCG 1 cut(s) 131
Bsh1285I CGRYCG 1 cut(s) 851
BshFI GGCC 1 cut(s) 199
BsiEI CGRYCG 1 cut(s) 851
BsiHKAI GWGCWC 2 cut(s) 110, 176
BsiHKCI CYCGRG 2 cut(s) 463, 534
BsiSI CCGG 3 cut(s) 71, 121, 535
BslFI GGGAC 1 cut(s) 446
BslI CCNNNNNNNGG 2 cut(s) 730, 757
BsmAI GTCTC 5 cut(s) 198, 303, 462, 612, 843
BsmBI CGTCTC 3 cut(s) 198, 612, 843
BsmFI GGGAC 1 cut(s) 446
BsmI GAATGC 1 cut(s) 367
BsnI GGCC 1 cut(s) 199
BsoBI CYCGRG 2 cut(s) 463, 534
Bsp1286I GDGCHC 2 cut(s) 110, 176
Bsp1407I TGTACA 1 cut(s) 811
Bsp143I GATC 5 cut(s) 142, 187, 221, 655, 973
BspACI CCGC 4 cut(s) 14, 115, 216, 625
BspANI GGCC 1 cut(s) 199
BspCNI CTCAG 4 cut(s) 181, 313, 428, 922
BspFNI CGCG 1 cut(s) 131
BspLI GGNNCC 1 cut(s) 614
BspOI GCTAGC 1 cut(s) 689
BspPI GGATC 2 cut(s) 182, 229
BspQI GCTCTTC 1 cut(s) 333
BsrDI GCAATG 2 cut(s) 254, 409
BsrGI TGTACA 1 cut(s) 811
BssECI CCNNGG 1 cut(s) 534
BssMI GATC 5 cut(s) 142, 187, 221, 655, 973
Bst6I CTCTTC 2 cut(s) 333, 383
BstAUI TGTACA 1 cut(s) 811
BstC8I GCNNGC 1 cut(s) 687
BstDEI CTNAG 4 cut(s) 168, 300, 415, 909
BstFNI CGCG 1 cut(s) 131
BstH2I RGCGCY 2 cut(s) 100, 583
BstHHI GCGC 3 cut(s) 99, 133, 582
BstKTI GATC 5 cut(s) 145, 190, 224, 658, 976
BstMAI GTCTC 5 cut(s) 198, 303, 462, 612, 843
BstMBI GATC 5 cut(s) 142, 187, 221, 655, 973
BstMCI CGRYCG 1 cut(s) 851
BstMWI GCNNNNNNNGC 7 cut(s) 11, 105, 114, 518, 530, 686, 863
BstSCI CCNGG 2 cut(s) 533, 534
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 4 cut(s) 17, 129, 159, 533
BsuRI GGCC 1 cut(s) 199
BtsI GCAGTG 1 cut(s) 680
BtsIMutI CAGTG 1 cut(s) 680
Cac8I GCNNGC 1 cut(s) 687
CfoI GCGC 3 cut(s) 99, 133, 582
Cfr9I CCCGGG 1 cut(s) 534
CseI GACGC 1 cut(s) 137
Csp6I GTAC 2 cut(s) 763, 812
CviAII CATG 3 cut(s) 55, 449, 968
CviQI GTAC 2 cut(s) 763, 812
DdeI CTNAG 4 cut(s) 168, 300, 415, 909
DpnI GATC 5 cut(s) 144, 189, 223, 657, 975
DpnII GATC 5 cut(s) 142, 187, 221, 655, 973
DriI GACNNNNNGTC 1 cut(s) 835
EaeI YGGCCR 1 cut(s) 197
Eam1104I CTCTTC 2 cut(s) 333, 383
Eam1105I GACNNNNNGTC 1 cut(s) 835
EarI CTCTTC 2 cut(s) 333, 383
EciI GGCGGA 1 cut(s) 231
Ecl136II GAGCTC 1 cut(s) 108
Eco24I GRGCYC 1 cut(s) 110
Eco47III AGCGCT 2 cut(s) 98, 581
Eco53kI GAGCTC 1 cut(s) 108
Eco57I CTGAAG 3 cut(s) 314, 500, 653
Eco88I CYCGRG 2 cut(s) 463, 534
EcoICRI GAGCTC 1 cut(s) 108
EcoT38I GRGCYC 1 cut(s) 110
Esp3I CGTCTC 3 cut(s) 198, 612, 843
FaeI CATG 3 cut(s) 58, 452, 971
FaiI YATR 8 cut(s) 56, 284, 450, 509, 572, 799, 801, 969
FaqI GGGAC 1 cut(s) 446
FatI CATG 3 cut(s) 54, 448, 967
FauI CCCGC 1 cut(s) 618
FauNDI CATATG 1 cut(s) 799
Fnu4HI GCNGC 4 cut(s) 6, 118, 148, 522
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 4 cut(s) 6, 118, 148, 522
FspBI CTAG 4 cut(s) 686, 704, 725, 827
GlaI GCGC 3 cut(s) 98, 132, 581
GluI GCNGC 4 cut(s) 6, 118, 148, 522
GsuI CTGGAG 2 cut(s) 124, 614
HaeII RGCGCY 2 cut(s) 100, 583
HaeIII GGCC 1 cut(s) 199
HapII CCGG 3 cut(s) 71, 121, 535
HgaI GACGC 1 cut(s) 137
HhaI GCGC 3 cut(s) 99, 133, 582
Hin1II CATG 3 cut(s) 58, 452, 971
Hin6I GCGC 3 cut(s) 97, 131, 580
HinP1I GCGC 3 cut(s) 97, 131, 580
HinfI GANTC 8 cut(s) 59, 251, 278, 344, 371, 434, 604, 794
HpaII CCGG 3 cut(s) 71, 121, 535
HphI GGTGA 4 cut(s) 440, 673, 766, 893
Hpy188I TCNGA 9 cut(s) 91, 171, 333, 349, 418, 609, 617, 793, 912
Hpy188III TCNNGA 4 cut(s) 103, 307, 463, 699
Hpy99I CGWCG 2 cut(s) 244, 404
HpyAV CCTTC 2 cut(s) 238, 334
HpyCH4V TGCA 2 cut(s) 365, 574
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 105, 114, 518, 530, 686, 863
HpyF3I CTNAG 4 cut(s) 168, 300, 415, 909
Hsp92II CATG 3 cut(s) 58, 452, 971
HspAI GCGC 3 cut(s) 97, 131, 580
Kzo9I GATC 5 cut(s) 142, 187, 221, 655, 973
LguI GCTCTTC 1 cut(s) 333
LmnI GCTCC 3 cut(s) 105, 155, 618
Lsp1109I GCAGC 4 cut(s) 17, 129, 159, 533
LweI GCATC 1 cut(s) 874
MaeI CTAG 4 cut(s) 686, 704, 725, 827
MaeIII GTNAC 1 cut(s) 48
MalI GATC 5 cut(s) 144, 189, 223, 657, 975
MboI GATC 5 cut(s) 142, 187, 221, 655, 973
MboII GAAGA 8 cut(s) 54, 57, 320, 362, 380, 400, 493, 662
MfeI CAATTG 1 cut(s) 162
MhlI GDGCHC 2 cut(s) 110, 176
MluCI AATT 4 cut(s) 162, 201, 395, 715
MlyI GAGTC 1 cut(s) 245
MmeI TCCRAC 1 cut(s) 361
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 3 cut(s) 71, 121, 535
MspR9I CCNGG 2 cut(s) 535, 536
MunI CAATTG 1 cut(s) 162
Mva1269I GAATGC 1 cut(s) 367
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 7 cut(s) 11, 105, 114, 518, 530, 686, 863
NciI CCSGG 2 cut(s) 535, 536
NdeI CATATG 1 cut(s) 799
NdeII GATC 5 cut(s) 142, 187, 221, 655, 973
NheI GCTAGC 1 cut(s) 685
NlaIII CATG 3 cut(s) 58, 452, 971
NlaIV GGNNCC 1 cut(s) 614
NmeAIII GCCGAG 2 cut(s) 175, 715
NmuCI GTSAC 1 cut(s) 48
PciSI GCTCTTC 1 cut(s) 333
PcsI WCGNNNNNNNCGW 1 cut(s) 613
PctI GAATGC 1 cut(s) 367
PfeI GAWTC 7 cut(s) 59, 278, 344, 371, 434, 604, 794
PflMI CCANNNNNTGG 1 cut(s) 757
PkrI GCNGC 4 cut(s) 7, 119, 149, 523
PleI GAGTC 1 cut(s) 245
PpsI GAGTC 1 cut(s) 245
Psp124BI GAGCTC 1 cut(s) 110
PspN4I GGNNCC 1 cut(s) 614
PsrI GAACNNNNNNTAC 2 cut(s) 795, 827
RsaI GTAC 2 cut(s) 764, 813
RsaNI GTAC 2 cut(s) 763, 812
SacI GAGCTC 1 cut(s) 110
SapI GCTCTTC 1 cut(s) 333
SatI GCNGC 4 cut(s) 6, 118, 148, 522
Sau3AI GATC 5 cut(s) 142, 187, 221, 655, 973
SchI GAGTC 1 cut(s) 245
ScrFI CCNGG 2 cut(s) 535, 536
SduI GDGCHC 2 cut(s) 110, 176
SfaNI GCATC 1 cut(s) 874
SmaI CCCGGG 1 cut(s) 536
SmlI CTYRAG 2 cut(s) 542, 644
SmoI CTYRAG 2 cut(s) 542, 644
Sse9I AATT 4 cut(s) 162, 201, 395, 715
SsiI CCGC 4 cut(s) 14, 115, 216, 625
SspMI CTAG 4 cut(s) 686, 704, 725, 827
SstI GAGCTC 1 cut(s) 110
StyD4I CCNGG 2 cut(s) 533, 534
TaqI TCGA 4 cut(s) 224, 308, 399, 548
TaqII GACCGA 1 cut(s) 865
TasI AATT 4 cut(s) 162, 201, 395, 715
TatI WGTACW 1 cut(s) 811
TfiI GAWTC 7 cut(s) 59, 278, 344, 371, 434, 604, 794
TscAI CASTG 1 cut(s) 687
TseFI GTSAC 1 cut(s) 48
TseI GCWGC 4 cut(s) 5, 117, 147, 521
Tsp45I GTSAC 1 cut(s) 48
TspDTI ATGAA 5 cut(s) 447, 726, 786, 816, 915
TspGWI ACGGA 1 cut(s) 180
TspMI CCCGGG 1 cut(s) 534
TspRI CASTG 1 cut(s) 687
Van91I CCANNNNNTGG 1 cut(s) 757
XapI RAATTY 2 cut(s) 395, 715
XmaI CCCGGG 1 cut(s) 534
XspI CTAG 4 cut(s) 686, 704, 725, 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.