MD11G1285200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0012509
Physical Location & Seq
Forward (+)
40356100 .. 40358013
1914 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1285200.v1.1.491

Sequence Viewer

Length: 1071 bp
ATGGCAGCTTCAGTGGACACGCCATCGCCTGCCCACCTCAGCAAGCAATCACTCATGGCTTCTTCTTCTCCGGTTTTCAGCCCCTCCTCCGATAAGCGCTTCTGGAGCTCTCTCCGCAGCCGGATCGATGCGCTTCTCGACGATCGGAACTCCAAAATCCCAATTGCTCAGAACCCACCAAATGCCGATCCGTCTCTGCCAATTCAAACGGAAAATAATGGCGGAGAAACAAAACCTGCGAGAGCGAGGAAGGAGGACTCGTTGCTTTTGATGAGAGGGTTCGATTCCATAGCTTACACTTTGTCTCAGCTCTCCAACACTTTGGATAATGCTCTTCAGGGAGCCAACGATCTAGCTAAGCCACCCACATTGACAGAGATATTCCATAGCCATCTGAGCAACTCGGAGGCGAAGAGTAAAGAGAAGGAATCGGAAGAACAGCAGAGTGCAGACGATTCCCATGTAGGATTGAAGAGGAAATTCGACGATAGTCATTGCTCAGAGGAACAAGGAGATGAATCCAAGAAAGAAAATGAGCACGAGCACGAAGGCCCGAAAGACGGGAAGCTGAAGAAAGCAAAAAATCTTGCAATTTCAATGGCAACAAAAGCAGCTTCGTTTGCCCGGGAACTCAAGTCGATAAGATCGGATTTATGCTTTATGCAAGAGCGCTGTGCTCTGCTGGAGGAGGAGAATCGGAGGCTCTGGGACGGGATGGATAAAGGGTTGAGGCCTGAGGAAGATGATCTGGTGAGGCTTCAGTTGGAGGCACTGCTAGCTGAGAAATCAAGACTAGCAAATGAAAATTCAAACCTGGTTAGGGAAAATCAATGCCTCCACCAGCTTGTGGAGTACCACCAGCTCACCTCCCAAGATCTCTCTGCTTCGTATGAACAAGTTGTACAAGGGTTGTGCCTAGACTTCTCGTCTCCTCCACGACCGACAGCAGACGAGGCAAATGGTGAAGATGGTGATGCTGCTGCTGATAATGAAGTTTATCAGACACCAAGAAGTAATCTTTTTGGCTTCTCTGCCTCGCTTGATGACGAGTGTTCTCGTGGAGATCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

357

Amino Acids

39.44

Weight (kDa)

4.93

Isoelectric Point (pI)

51.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012509)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02800
fragaria_vesca FvH4_2g23580 FvH4_2g23580
malus_domestica MD03G1265000.v1.1 MD11G1285200.v1.1
prunus_persica Prupe.8G241100_v2.0.a1
pyrus_communis pycom03g21210 pycom11g25280 pycom11g25340
rosa_chinensis RchiOBHm_Chr6g0291051
rosa_laevigata RLG00000012151
rosa_multiflora Rmu_sc0004642.1_g000003
rosa_roxburghii Rroxscaffold_7G00176420
rosa_rugosa Rorug06G0217200
rosa_samantha Rh6AG327300 Rh6BG334800 Rh6CG341000 Rh6DG328300
rosa_wichuraiana Rw6G028470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 244
AccB7I CCANNNNNTGG 1 cut(s) 847
AciI CCGC 2 cut(s) 115, 222
AclWI GGATC 2 cut(s) 131, 182
AcsI RAATTY 2 cut(s) 479, 805
AcuI CTGAAG 3 cut(s) 320, 590, 743
AfaI GTAC 2 cut(s) 854, 903
AfeI AGCGCT 2 cut(s) 98, 671
AfiI CCNNNNNNNGG 3 cut(s) 560, 820, 847
AgsI TTSAA 4 cut(s) 206, 472, 597, 810
AhdI GACNNNNNGTC 1 cut(s) 925
AjnI CCWGG 1 cut(s) 813
Alw21I GWGCWC 4 cut(s) 110, 540, 546, 679
Alw26I GTCTC 3 cut(s) 198, 309, 933
AlwI GGATC 2 cut(s) 131, 182
Ama87I CYCGRG 1 cut(s) 624
Aor51HI AGCGCT 2 cut(s) 98, 671
AoxI GGCC 2 cut(s) 550, 731
ApeKI GCWGC 5 cut(s) 5, 117, 611, 977, 980
ApoI RAATTY 2 cut(s) 479, 805
AspLEI GCGC 3 cut(s) 99, 133, 672
AspS9I GGNCC 1 cut(s) 551
AsuC2I CCSGG 2 cut(s) 625, 626
AsuHPI GGTGA 4 cut(s) 763, 856, 974, 983
AsuNHI GCTAGC 1 cut(s) 775
AvaI CYCGRG 1 cut(s) 624
AxyI CCTNAGG 1 cut(s) 735
BanII GRGCYC 1 cut(s) 110
BauI CACGAG 2 cut(s) 539, 1056
Bbv12I GWGCWC 4 cut(s) 110, 540, 546, 679
BbvCI CCTCAGC 1 cut(s) 38
BbvI GCAGC 5 cut(s) 17, 129, 623, 964, 967
BccI CCATC 4 cut(s) 31, 399, 709, 962
BcgI CGANNNNNNTGC 2 cut(s) 106, 140
BciT130I CCWGG 1 cut(s) 815
BcnI CCSGG 2 cut(s) 625, 626
BcoDI GTCTC 3 cut(s) 198, 309, 933
BfaI CTAG 4 cut(s) 353, 776, 794, 917
BfoI RGCGCY 2 cut(s) 100, 673
BfuAI ACCTGC 1 cut(s) 244
BglII AGATCT 1 cut(s) 874
BisI GCNGC 5 cut(s) 6, 118, 612, 978, 981
BlpI GCTNAGC 1 cut(s) 357
BlsI GCNGC 5 cut(s) 7, 119, 613, 979, 982
Bme1390I CCNGG 3 cut(s) 625, 626, 815
BmeRI GACNNNNNGTC 1 cut(s) 925
BmeT110I CYCGRG 1 cut(s) 624
BmgT120I GGNCC 1 cut(s) 551
BmiI GGNNCC 1 cut(s) 343
BmrFI CCNGG 3 cut(s) 625, 626, 815
BmsI GCATC 2 cut(s) 118, 964
BmtI GCTAGC 1 cut(s) 779
BoxI GACNNNNGTC 1 cut(s) 489
BpmI CTGGAG 2 cut(s) 124, 704
Bpu10I CCTNAGC 1 cut(s) 38
Bpu1102I GCTNAGC 1 cut(s) 357
BpuEI CTTGAG 1 cut(s) 617
BpuMI CCSGG 2 cut(s) 625, 626
Bsa29I ATCGAT 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 624
BsaWI WCCGGW 1 cut(s) 70
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 3 cut(s) 560, 820, 847
Bse21I CCTNAGG 1 cut(s) 735
Bse3DI GCAATG 1 cut(s) 493
BseBI CCWGG 1 cut(s) 815
BseCI ATCGAT 1 cut(s) 126
BseDI CCNNGG 1 cut(s) 624
BseGI GGATG 1 cut(s) 720
BseLI CCNNNNNNNGG 3 cut(s) 560, 820, 847
BseMI GCAATG 1 cut(s) 493
BseMII CTCAG 7 cut(s) 52, 182, 320, 386, 513, 726, 771
BseRI GAGGAG 4 cut(s) 76, 701, 704, 921
BseXI GCAGC 5 cut(s) 17, 129, 623, 964, 967
BsgI GTGCAG 1 cut(s) 468
Bsh1285I CGRYCG 2 cut(s) 145, 941
BshFI GGCC 2 cut(s) 552, 733
BshVI ATCGAT 1 cut(s) 126
BsiEI CGRYCG 2 cut(s) 145, 941
BsiHKAI GWGCWC 4 cut(s) 110, 540, 546, 679
BsiHKCI CYCGRG 1 cut(s) 624
BsiSI CCGG 3 cut(s) 71, 121, 625
BslFI GGGAC 1 cut(s) 722
BslI CCNNNNNNNGG 3 cut(s) 560, 820, 847
BsmAI GTCTC 3 cut(s) 198, 309, 933
BsmBI CGTCTC 2 cut(s) 198, 933
BsmFI GGGAC 1 cut(s) 722
BsnI GGCC 2 cut(s) 552, 733
BsoBI CYCGRG 1 cut(s) 624
Bsp1286I GDGCHC 4 cut(s) 110, 540, 546, 679
Bsp1407I TGTACA 1 cut(s) 901
Bsp143I GATC 8 cut(s) 123, 142, 187, 349, 644, 745, 874, 1063
Bsp1720I GCTNAGC 1 cut(s) 357
BspACI CCGC 2 cut(s) 115, 222
BspANI GGCC 2 cut(s) 552, 733
BspCNI CTCAG 7 cut(s) 51, 181, 319, 387, 512, 727, 772
BspDI ATCGAT 1 cut(s) 126
BspLI GGNNCC 1 cut(s) 343
BspMI ACCTGC 1 cut(s) 244
BspOI GCTAGC 1 cut(s) 779
BspPI GGATC 2 cut(s) 131, 182
BspQI GCTCTTC 1 cut(s) 339
BsrDI GCAATG 1 cut(s) 493
BsrGI TGTACA 1 cut(s) 901
BssECI CCNNGG 1 cut(s) 624
BssMI GATC 8 cut(s) 123, 142, 187, 349, 644, 745, 874, 1063
BssSI CACGAG 2 cut(s) 539, 1056
Bst2BI CACGAG 2 cut(s) 539, 1056
Bst2UI CCWGG 1 cut(s) 815
Bst6I CTCTTC 3 cut(s) 339, 407, 467
BstAUI TGTACA 1 cut(s) 901
BstC8I GCNNGC 3 cut(s) 30, 44, 777
BstDEI CTNAG 8 cut(s) 38, 168, 306, 357, 395, 499, 735, 780
BstF5I GGATG 1 cut(s) 720
BstH2I RGCGCY 2 cut(s) 100, 673
BstHHI GCGC 3 cut(s) 99, 133, 672
BstKTI GATC 8 cut(s) 126, 145, 190, 352, 647, 748, 877, 1066
BstMAI GTCTC 3 cut(s) 198, 309, 933
BstMBI GATC 8 cut(s) 123, 142, 187, 349, 644, 745, 874, 1063
BstMCI CGRYCG 2 cut(s) 145, 941
BstMWI GCNNNNNNNGC 7 cut(s) 105, 114, 396, 608, 620, 776, 953
BstNI CCWGG 1 cut(s) 815
BstPAI GACNNNNGTC 1 cut(s) 489
BstSCI CCNGG 3 cut(s) 623, 624, 813
BstV1I GCAGC 5 cut(s) 17, 129, 623, 964, 967
BstX2I RGATCY 1 cut(s) 874
BstXI CCANNNNNNTGG 1 cut(s) 322
BstYI RGATCY 1 cut(s) 874
Bsu15I ATCGAT 1 cut(s) 126
Bsu36I CCTNAGG 1 cut(s) 735
BsuRI GGCC 2 cut(s) 552, 733
BsuTUI ATCGAT 1 cut(s) 126
BtgZI GCGATG 1 cut(s) 9
BtsCI GGATG 1 cut(s) 720
BtsI GCAGTG 1 cut(s) 770
BtsIMutI CAGTG 2 cut(s) 18, 770
BveI ACCTGC 1 cut(s) 244
Cac8I GCNNGC 3 cut(s) 30, 44, 777
CfoI GCGC 3 cut(s) 99, 133, 672
Cfr13I GGNCC 1 cut(s) 551
Cfr9I CCCGGG 1 cut(s) 624
ClaI ATCGAT 1 cut(s) 126
CsiI ACCWGGT 1 cut(s) 813
Csp6I GTAC 2 cut(s) 853, 902
CspCI CAANNNNNGTGG 2 cut(s) 351, 386
CviAII CATG 2 cut(s) 55, 461
CviQI GTAC 2 cut(s) 853, 902
DdeI CTNAG 8 cut(s) 38, 168, 306, 357, 395, 499, 735, 780
DpnI GATC 8 cut(s) 125, 144, 189, 351, 646, 747, 876, 1065
DpnII GATC 8 cut(s) 123, 142, 187, 349, 644, 745, 874, 1063
DriI GACNNNNNGTC 1 cut(s) 925
Eam1104I CTCTTC 3 cut(s) 339, 407, 467
Eam1105I GACNNNNNGTC 1 cut(s) 925
EarI CTCTTC 3 cut(s) 339, 407, 467
EciI GGCGGA 1 cut(s) 237
Ecl136II GAGCTC 1 cut(s) 108
Eco147I AGGCCT 1 cut(s) 733
Eco24I GRGCYC 1 cut(s) 110
Eco47III AGCGCT 2 cut(s) 98, 671
Eco53kI GAGCTC 1 cut(s) 108
Eco57I CTGAAG 3 cut(s) 320, 590, 743
Eco81I CCTNAGG 1 cut(s) 735
Eco88I CYCGRG 1 cut(s) 624
EcoICRI GAGCTC 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 813
EcoT38I GRGCYC 1 cut(s) 110
Esp3I CGTCTC 2 cut(s) 198, 933
FaeI CATG 2 cut(s) 58, 464
FaiI YATR 7 cut(s) 56, 290, 387, 462, 655, 662, 891
FaqI GGGAC 1 cut(s) 722
FatI CATG 2 cut(s) 54, 460
Fnu4HI GCNGC 5 cut(s) 6, 118, 612, 978, 981
FokI GGATG 1 cut(s) 727
FriOI GRGCYC 1 cut(s) 110
Fsp4HI GCNGC 5 cut(s) 6, 118, 612, 978, 981
FspBI CTAG 4 cut(s) 353, 776, 794, 917
GlaI GCGC 3 cut(s) 98, 132, 671
GluI GCNGC 5 cut(s) 6, 118, 612, 978, 981
GsuI CTGGAG 2 cut(s) 124, 704
HaeII RGCGCY 2 cut(s) 100, 673
HaeIII GGCC 2 cut(s) 552, 733
HapII CCGG 3 cut(s) 71, 121, 625
HhaI GCGC 3 cut(s) 99, 133, 672
Hin1II CATG 2 cut(s) 58, 464
Hin6I GCGC 3 cut(s) 97, 131, 670
HinP1I GCGC 3 cut(s) 97, 131, 670
HinfI GANTC 6 cut(s) 257, 284, 428, 455, 518, 694
HpaII CCGG 3 cut(s) 71, 121, 625
HphI GGTGA 4 cut(s) 763, 856, 974, 983
Hpy166II GTNNAC 1 cut(s) 16
Hpy188III TCNNGA 3 cut(s) 103, 137, 789
Hpy8I GTNNAC 1 cut(s) 16
Hpy99I CGWCG 2 cut(s) 143, 488
HpyAV CCTTC 3 cut(s) 244, 418, 542
HpyCH4V TGCA 3 cut(s) 449, 590, 664
HpyF10VI GCNNNNNNNGC 7 cut(s) 105, 114, 396, 608, 620, 776, 953
HpyF3I CTNAG 8 cut(s) 38, 168, 306, 357, 395, 499, 735, 780
Hsp92II CATG 2 cut(s) 58, 464
HspAI GCGC 3 cut(s) 97, 131, 670
Kzo9I GATC 8 cut(s) 123, 142, 187, 349, 644, 745, 874, 1063
LguI GCTCTTC 1 cut(s) 339
LmnI GCTCC 2 cut(s) 105, 341
Lsp1109I GCAGC 5 cut(s) 17, 129, 623, 964, 967
LweI GCATC 2 cut(s) 118, 964
MabI ACCWGGT 1 cut(s) 813
MaeI CTAG 4 cut(s) 353, 776, 794, 917
MalI GATC 8 cut(s) 125, 144, 189, 351, 646, 747, 876, 1065
MboI GATC 8 cut(s) 123, 142, 187, 349, 644, 745, 874, 1063
MboII GAAGA 9 cut(s) 54, 57, 326, 424, 446, 484, 583, 752, 977
MfeI CAATTG 1 cut(s) 162
MflI RGATCY 1 cut(s) 874
MhlI GDGCHC 4 cut(s) 110, 540, 546, 679
MluCI AATT 5 cut(s) 162, 201, 479, 591, 805
MlyI GAGTC 1 cut(s) 251
MmeI TCCRAC 2 cut(s) 339, 744
MspI CCGG 3 cut(s) 71, 121, 625
MspR9I CCNGG 3 cut(s) 625, 626, 815
MunI CAATTG 1 cut(s) 162
MvaI CCWGG 1 cut(s) 815
MwoI GCNNNNNNNGC 7 cut(s) 105, 114, 396, 608, 620, 776, 953
NciI CCSGG 2 cut(s) 625, 626
NdeII GATC 8 cut(s) 123, 142, 187, 349, 644, 745, 874, 1063
NheI GCTAGC 1 cut(s) 775
NlaIII CATG 2 cut(s) 58, 464
NlaIV GGNNCC 1 cut(s) 343
PceI AGGCCT 1 cut(s) 733
PciSI GCTCTTC 1 cut(s) 339
PfeI GAWTC 5 cut(s) 284, 428, 455, 518, 694
PflMI CCANNNNNTGG 1 cut(s) 847
PkrI GCNGC 5 cut(s) 7, 119, 613, 979, 982
Ple19I CGATCG 1 cut(s) 145
PleI GAGTC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 251
PshAI GACNNNNGTC 1 cut(s) 489
Psp124BI GAGCTC 1 cut(s) 110
Psp6I CCWGG 1 cut(s) 813
PspGI CCWGG 1 cut(s) 813
PspN4I GGNNCC 1 cut(s) 343
PspPI GGNCC 1 cut(s) 551
PsrI GAACNNNNNNTAC 2 cut(s) 885, 917
PsuI RGATCY 1 cut(s) 874
PvuI CGATCG 1 cut(s) 145
RsaI GTAC 2 cut(s) 854, 903
RsaNI GTAC 2 cut(s) 853, 902
SacI GAGCTC 1 cut(s) 110
SapI GCTCTTC 1 cut(s) 339
SatI GCNGC 5 cut(s) 6, 118, 612, 978, 981
Sau3AI GATC 8 cut(s) 123, 142, 187, 349, 644, 745, 874, 1063
Sau96I GGNCC 1 cut(s) 551
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 3 cut(s) 625, 626, 815
SduI GDGCHC 4 cut(s) 110, 540, 546, 679
SexAI ACCWGGT 1 cut(s) 813
SfaNI GCATC 2 cut(s) 118, 964
SmaI CCCGGG 1 cut(s) 626
SmlI CTYRAG 1 cut(s) 632
SmoI CTYRAG 1 cut(s) 632
Sse9I AATT 5 cut(s) 162, 201, 479, 591, 805
SseBI AGGCCT 1 cut(s) 733
SsiI CCGC 2 cut(s) 115, 222
SspMI CTAG 4 cut(s) 353, 776, 794, 917
SstI GAGCTC 1 cut(s) 110
StuI AGGCCT 1 cut(s) 733
StyD4I CCNGG 3 cut(s) 623, 624, 813
TaqI TCGA 5 cut(s) 126, 138, 282, 483, 638
TaqII GACCGA 1 cut(s) 955
TasI AATT 5 cut(s) 162, 201, 479, 591, 805
TatI WGTACW 1 cut(s) 901
TfiI GAWTC 5 cut(s) 284, 428, 455, 518, 694
TscAI CASTG 2 cut(s) 18, 777
TseI GCWGC 5 cut(s) 5, 117, 611, 977, 980
TspDTI ATGAA 4 cut(s) 531, 816, 906, 1005
TspGWI ACGGA 2 cut(s) 180, 224
TspMI CCCGGG 1 cut(s) 624
TspRI CASTG 2 cut(s) 18, 777
Van91I CCANNNNNTGG 1 cut(s) 847
XapI RAATTY 2 cut(s) 479, 805
XmaI CCCGGG 1 cut(s) 624
XspI CTAG 4 cut(s) 353, 776, 794, 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.