pycom03g21210

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0012509
Physical Location & Seq
Forward (+)
21524488 .. 21525686
1199 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g21210.2

Sequence Viewer

Length: 777 bp
ATGAGAGGGTTTGATTCCATAGCTCACACTTTGGCTCAGCTCTCGAATACTTTGGATAATGCTCTTCAGGGAGCCAACGGTCTAGCTAAGCCACCCACATTGACGGAGATATTACATAACCACCTGAGCAACTCGGAGACGAAGAGTAGAGAAAAGGATTCGGAAGAACAGCAGAATGCAGAAGATTCCAATGTTGGATTGAAGAGGAAATTCGATGACAGCCATTGCTCAGAGGAACAAGGAGATGAATCCCAGAAAGAAAATGAGAAAGAAGGCCCGAGAGAGGGGAAGCTGAAGGAAGCAAAAAATCTTGCGATTTCTATGGCAACAAAAGCAGCTTCGTTTGCCCGGGAACTCAAGTCATTAAGGTTAGATCTATCCTTTATGCAAGAGCGCTGTGTTTTGCTGGAGGAGGAGAATCGGAGGCTCCGAGACGGGCTGGATAAAGGGTCGAGGCCTGAGGAAGATGATCTGGTGACGCTTCAGTTGGAGGCACTGCTAGCTGAGAAATCAAGACTAGCAAATGAAAATTCAAACCTAGTTAGGGAAAATCAATGCCTCCACCAGCTTGTGGAGTACCACCAGCTCACCTCCCAAGACCTCTCTGATTCATATGAACAAGTTGTACAAGGGTTGTGCCTAGACTTCTCGTCTCCTCCACGACCGACAGCAGACGAGGCAAATGGTGGAGATGGTGATGCTGCTGCTGATAATGAAGTTTCTCAGACACCAAGAAGTAATCTTTTAGGGTTCTCTGCTTCGCTTGATGATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

259

Amino Acids

28.58

Weight (kDa)

4.66

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012509)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02800
fragaria_vesca FvH4_2g23580 FvH4_2g23580
malus_domestica MD03G1265000.v1.1 MD11G1285200.v1.1
prunus_persica Prupe.8G241100_v2.0.a1
pyrus_communis pycom03g21210 pycom11g25280 pycom11g25340
rosa_chinensis RchiOBHm_Chr6g0291051
rosa_laevigata RLG00000012151
rosa_multiflora Rmu_sc0004642.1_g000003
rosa_roxburghii Rroxscaffold_7G00176420
rosa_rugosa Rorug06G0217200
rosa_samantha Rh6AG327300 Rh6BG334800 Rh6CG341000 Rh6DG328300
rosa_wichuraiana Rw6G028470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 571
AcsI RAATTY 2 cut(s) 209, 529
AcuI CTGAAG 3 cut(s) 50, 314, 467
AfaI GTAC 2 cut(s) 578, 627
AfeI AGCGCT 1 cut(s) 395
AfiI CCNNNNNNNGG 4 cut(s) 283, 284, 544, 571
AgsI TTSAA 2 cut(s) 202, 534
AhdI GACNNNNNGTC 1 cut(s) 649
AluBI AGCT 8 cut(s) 23, 40, 86, 292, 338, 503, 568, 586
AluI AGCT 8 cut(s) 23, 40, 86, 292, 338, 503, 568, 586
Alw26I GTCTC 3 cut(s) 131, 426, 657
Ama87I CYCGRG 2 cut(s) 277, 348
Aor51HI AGCGCT 1 cut(s) 395
AoxI GGCC 2 cut(s) 274, 455
ApeKI GCWGC 3 cut(s) 335, 701, 704
ApoI RAATTY 2 cut(s) 209, 529
AspLEI GCGC 1 cut(s) 396
AspS9I GGNCC 1 cut(s) 275
AsuC2I CCSGG 2 cut(s) 349, 350
AsuHPI GGTGA 3 cut(s) 487, 580, 707
AsuNHI GCTAGC 1 cut(s) 499
AvaI CYCGRG 2 cut(s) 277, 348
AxyI CCTNAGG 1 cut(s) 459
BbvI GCAGC 3 cut(s) 347, 688, 691
BccI CCATC 1 cut(s) 686
BclI TGATCA 1 cut(s) 769
BcnI CCSGG 2 cut(s) 349, 350
BcoDI GTCTC 3 cut(s) 131, 426, 657
BfaI CTAG 5 cut(s) 83, 500, 518, 539, 641
BfoI RGCGCY 1 cut(s) 397
BglII AGATCT 1 cut(s) 373
BisI GCNGC 3 cut(s) 336, 702, 705
BlpI GCTNAGC 2 cut(s) 36, 87
BlsI GCNGC 3 cut(s) 337, 703, 706
Bme1390I CCNGG 2 cut(s) 349, 350
BmeRI GACNNNNNGTC 1 cut(s) 649
BmeT110I CYCGRG 2 cut(s) 277, 348
BmgT120I GGNCC 1 cut(s) 275
BmiI GGNNCC 2 cut(s) 73, 428
BmrFI CCNGG 2 cut(s) 349, 350
BmsI GCATC 1 cut(s) 688
BmtI GCTAGC 1 cut(s) 503
BpmI CTGGAG 1 cut(s) 428
Bpu10I CCTNAGC 1 cut(s) 125
Bpu1102I GCTNAGC 2 cut(s) 36, 87
BpuEI CTTGAG 1 cut(s) 341
BpuMI CCSGG 2 cut(s) 349, 350
BsaJI CCNNGG 1 cut(s) 348
Bsc4I CCNNNNNNNGG 4 cut(s) 283, 284, 544, 571
Bse21I CCTNAGG 1 cut(s) 459
Bse3DI GCAATG 1 cut(s) 223
BseDI CCNNGG 1 cut(s) 348
BseLI CCNNNNNNNGG 4 cut(s) 283, 284, 544, 571
BseMI GCAATG 1 cut(s) 223
BseMII CTCAG 6 cut(s) 50, 116, 243, 450, 495, 737
BseRI GAGGAG 3 cut(s) 425, 428, 645
BseXI GCAGC 3 cut(s) 347, 688, 691
Bsh1285I CGRYCG 1 cut(s) 665
BshFI GGCC 2 cut(s) 276, 457
BsiEI CGRYCG 1 cut(s) 665
BsiHKCI CYCGRG 2 cut(s) 277, 348
BsiSI CCGG 1 cut(s) 349
BslI CCNNNNNNNGG 4 cut(s) 283, 284, 544, 571
BsmAI GTCTC 3 cut(s) 131, 426, 657
BsmBI CGTCTC 3 cut(s) 131, 426, 657
BsmI GAATGC 1 cut(s) 181
BsnI GGCC 2 cut(s) 276, 457
BsoBI CYCGRG 2 cut(s) 277, 348
Bsp1407I TGTACA 1 cut(s) 625
Bsp143I GATC 3 cut(s) 373, 469, 769
Bsp1720I GCTNAGC 2 cut(s) 36, 87
BspANI GGCC 2 cut(s) 276, 457
BspCNI CTCAG 6 cut(s) 49, 117, 242, 451, 496, 736
BspLI GGNNCC 2 cut(s) 73, 428
BspOI GCTAGC 1 cut(s) 503
BspQI GCTCTTC 1 cut(s) 69
BsrDI GCAATG 1 cut(s) 223
BsrGI TGTACA 1 cut(s) 625
BssECI CCNNGG 1 cut(s) 348
BssMI GATC 3 cut(s) 373, 469, 769
Bst4CI ACNGT 1 cut(s) 80
Bst6I CTCTTC 3 cut(s) 69, 137, 197
BstAUI TGTACA 1 cut(s) 625
BstC8I GCNNGC 1 cut(s) 501
BstDEI CTNAG 7 cut(s) 36, 87, 125, 229, 459, 504, 723
BstH2I RGCGCY 1 cut(s) 397
BstHHI GCGC 1 cut(s) 396
BstKTI GATC 3 cut(s) 376, 472, 772
BstMAI GTCTC 3 cut(s) 131, 426, 657
BstMBI GATC 3 cut(s) 373, 469, 769
BstMCI CGRYCG 1 cut(s) 665
BstMWI GCNNNNNNNGC 4 cut(s) 332, 344, 500, 677
BstSCI CCNGG 2 cut(s) 347, 348
BstV1I GCAGC 3 cut(s) 347, 688, 691
BstX2I RGATCY 1 cut(s) 373
BstYI RGATCY 1 cut(s) 373
Bsu36I CCTNAGG 1 cut(s) 459
BsuRI GGCC 2 cut(s) 276, 457
BtsI GCAGTG 1 cut(s) 494
BtsIMutI CAGTG 1 cut(s) 494
Cac8I GCNNGC 1 cut(s) 501
CfoI GCGC 1 cut(s) 396
Cfr13I GGNCC 1 cut(s) 275
Cfr9I CCCGGG 1 cut(s) 348
CseI GACGC 1 cut(s) 487
Csp6I GTAC 2 cut(s) 577, 626
CspCI CAANNNNNGTGG 2 cut(s) 81, 116
CviQI GTAC 2 cut(s) 577, 626
DdeI CTNAG 7 cut(s) 36, 87, 125, 229, 459, 504, 723
DpnI GATC 3 cut(s) 375, 471, 771
DpnII GATC 3 cut(s) 373, 469, 769
DriI GACNNNNNGTC 1 cut(s) 649
Eam1104I CTCTTC 3 cut(s) 69, 137, 197
Eam1105I GACNNNNNGTC 1 cut(s) 649
EarI CTCTTC 3 cut(s) 69, 137, 197
Eco147I AGGCCT 1 cut(s) 457
Eco47III AGCGCT 1 cut(s) 395
Eco57I CTGAAG 3 cut(s) 50, 314, 467
Eco81I CCTNAGG 1 cut(s) 459
Eco88I CYCGRG 2 cut(s) 277, 348
Esp3I CGTCTC 3 cut(s) 131, 426, 657
FaiI YATR 6 cut(s) 20, 117, 323, 386, 613, 615
FauNDI CATATG 1 cut(s) 613
FbaI TGATCA 1 cut(s) 769
Fnu4HI GCNGC 3 cut(s) 336, 702, 705
Fsp4HI GCNGC 3 cut(s) 336, 702, 705
FspBI CTAG 5 cut(s) 83, 500, 518, 539, 641
GlaI GCGC 1 cut(s) 395
GluI GCNGC 3 cut(s) 336, 702, 705
GsuI CTGGAG 1 cut(s) 428
HaeII RGCGCY 1 cut(s) 397
HaeIII GGCC 2 cut(s) 276, 457
HapII CCGG 1 cut(s) 349
HgaI GACGC 1 cut(s) 487
HhaI GCGC 1 cut(s) 396
Hin6I GCGC 1 cut(s) 394
HinP1I GCGC 1 cut(s) 394
HinfI GANTC 6 cut(s) 14, 158, 185, 248, 418, 608
HpaII CCGG 1 cut(s) 349
HphI GGTGA 3 cut(s) 487, 580, 707
Hpy188I TCNGA 7 cut(s) 136, 163, 232, 423, 431, 607, 726
Hpy188III TCNNGA 2 cut(s) 43, 513
HpyAV CCTTC 2 cut(s) 266, 289
HpyCH4III ACNGT 1 cut(s) 80
HpyCH4V TGCA 2 cut(s) 179, 388
HpyF10VI GCNNNNNNNGC 4 cut(s) 332, 344, 500, 677
HpyF3I CTNAG 7 cut(s) 36, 87, 125, 229, 459, 504, 723
HspAI GCGC 1 cut(s) 394
Ksp22I TGATCA 1 cut(s) 769
Kzo9I GATC 3 cut(s) 373, 469, 769
LguI GCTCTTC 1 cut(s) 69
LmnI GCTCC 2 cut(s) 71, 432
Lsp1109I GCAGC 3 cut(s) 347, 688, 691
LweI GCATC 1 cut(s) 688
MaeI CTAG 5 cut(s) 83, 500, 518, 539, 641
MaeIII GTNAC 1 cut(s) 475
MalI GATC 3 cut(s) 375, 471, 771
MboI GATC 3 cut(s) 373, 469, 769
MboII GAAGA 6 cut(s) 56, 154, 176, 194, 214, 476
MflI RGATCY 1 cut(s) 373
MluCI AATT 2 cut(s) 209, 529
MmeI TCCRAC 2 cut(s) 175, 468
MseI TTAA 1 cut(s) 365
MspI CCGG 1 cut(s) 349
MspR9I CCNGG 2 cut(s) 349, 350
Mva1269I GAATGC 1 cut(s) 181
MwoI GCNNNNNNNGC 4 cut(s) 332, 344, 500, 677
NciI CCSGG 2 cut(s) 349, 350
NdeI CATATG 1 cut(s) 613
NdeII GATC 3 cut(s) 373, 469, 769
NheI GCTAGC 1 cut(s) 499
NlaIV GGNNCC 2 cut(s) 73, 428
NmuCI GTSAC 1 cut(s) 475
PceI AGGCCT 1 cut(s) 457
PciSI GCTCTTC 1 cut(s) 69
PcsI WCGNNNNNNNCGW 1 cut(s) 427
PctI GAATGC 1 cut(s) 181
PfeI GAWTC 6 cut(s) 14, 158, 185, 248, 418, 608
PflMI CCANNNNNTGG 1 cut(s) 571
PkrI GCNGC 3 cut(s) 337, 703, 706
PspN4I GGNNCC 2 cut(s) 73, 428
PspPI GGNCC 1 cut(s) 275
PsrI GAACNNNNNNTAC 2 cut(s) 609, 641
PsuI RGATCY 1 cut(s) 373
RsaI GTAC 2 cut(s) 578, 627
RsaNI GTAC 2 cut(s) 577, 626
SapI GCTCTTC 1 cut(s) 69
SaqAI TTAA 1 cut(s) 365
SatI GCNGC 3 cut(s) 336, 702, 705
Sau3AI GATC 3 cut(s) 373, 469, 769
Sau96I GGNCC 1 cut(s) 275
ScrFI CCNGG 2 cut(s) 349, 350
SfaNI GCATC 1 cut(s) 688
SmaI CCCGGG 1 cut(s) 350
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 2 cut(s) 209, 529
SseBI AGGCCT 1 cut(s) 457
SspMI CTAG 5 cut(s) 83, 500, 518, 539, 641
StuI AGGCCT 1 cut(s) 457
StyD4I CCNGG 2 cut(s) 347, 348
TaaI ACNGT 1 cut(s) 80
TaqI TCGA 3 cut(s) 44, 213, 452
TaqII GACCGA 1 cut(s) 679
TasI AATT 2 cut(s) 209, 529
TatI WGTACW 1 cut(s) 625
TfiI GAWTC 6 cut(s) 14, 158, 185, 248, 418, 608
Tru1I TTAA 1 cut(s) 365
Tru9I TTAA 1 cut(s) 365
TscAI CASTG 1 cut(s) 501
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 3 cut(s) 335, 701, 704
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 5 cut(s) 261, 540, 600, 630, 729
TspGWI ACGGA 1 cut(s) 119
TspMI CCCGGG 1 cut(s) 348
TspRI CASTG 1 cut(s) 501
Van91I CCANNNNNTGG 1 cut(s) 571
XapI RAATTY 2 cut(s) 209, 529
XmaI CCCGGG 1 cut(s) 348
XspI CTAG 5 cut(s) 83, 500, 518, 539, 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.