MD03G1277000.v1.1

Sodium hydrogen exchanger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007582
Physical Location & Seq
Reverse (-)
35949775 .. 35954850
5076 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1277000.v1.1.491

Sequence Viewer

Length: 1638 bp
ATGGAGGTCTCTACTTTGGTGAGATTGGGGGTGAGATCGGCCACCAGTGAAGCTTCCGTAGATTCGATCACTTTGTTCGTGGTTCTCCTATGTTTATGCATTGTGATTGGTCATCTTCTGGAGAAGGCTCGATGGATGACGGAATCGGTAACAGCGCTTCTCATTGGTCTTTGTATTGGAATTGTTATTCTACTAACTACCAAGGGGAAAAGCTCACACATCTTAGTATTTAATGAAGATCTCTTCTTTATATATCTTCTGCCGCCTATTATATTTAACGCCGGATTTCAGGTGAAAAAGAAGCGGTTTTTCCAGAACTTCATGACAATCACGTCATTGGGTGCTGTCGGCAGTTTAGTATCATTCGTAATAATATGGCTAGGTTCAATGCATTTGTTTAAAAATTTGGACGTTGGTTTCCTTGAGATGGGAGATTATCTAGCACTCGGAGCAATCTTCTCGGCTACAGATTCAGTTTGCACCCTGCAAGTGCTTAATCAAGATGAGACACCTTTACTCTACAGTCTAGTTTTTGGAGAAGGTGTTGTAAATGATGCCACATCTGTGGTACTTTTCAACGCAATTCAGAAATTTGATCTCTCTCGCATCAACTCAACCATTGCCTTGAAGTTTAGTGGCAATTTCGTATTTCTGTTGTTGACAAGCATGGTGCTTGGTGCAGCGGTTGGATTGCTTAGCGCGTACATCATAAAGAAGTTGTACCTCGGAAGGCACTCGACTGATCGTGAAGTTGCACTTATGATTCTAATGGCTTATTTGTCATATATGGCGGCAGAACTATTCAATCTAAGTGGTATTCTTACCGTTTTCTTTTGCGGGCTTGTCATGTCACACTATACCTGGCATAATGTAACTGAAAGTTCAAGAGTTACAACCAAGCATGCTTTTGCAACGTTCTCGTTTGTTTCTGAGATCTTCATCTTTCTATATGTTGGTATGGATGCCTTGGACATTGAGAAGTGGAAATTCGTGAAGGAGAGCCCTGGGACATCAATTGGAGTCAGTTCGATATTACTTGGCTTAGTTCTTGTTGGAAGAGCAGCTTTTGTATTTCCTCTATGTTTCTTATCAAACTTAACCAGGACATCTCAGATTGACAAAATCGGGTTTAAGCAACAGGTGACAGTATGGTGGGCAGGACTCATGCGTGGTGCTGTATCCGTGGCTCTTGCTTATAATTGGTTTGCAATGTCAACGCATACTCAACAGCGTGGAATTGCAATCATGATCACTAGTACGATAACAGTAGTTCTCTTCACTAATGTGGTGTGCGGATTATTGACTAAGCCTCTCGTAAGGTTCTTGTTGCCGCAACAGAATCACTTAGATAGCATGATGATGTCCTCTGAGTCGGAGATGTCTCTGGCTATGCCGCTCCTCGCTAATGAGGAAGATCCAAAGTCAAATATGTCTAGCGATAACATTCCTCGTCCATCCAGCCTGCGCATGCTCTTGACGACACCAACTCGTTCTGTCCATTACTATTGGAGAATGTTTGATGATGGTTACATGCGTCCTGTCTTTGGCGGTAGAGGATTCGTTCCTTATGCTGCCGGTTCACCCAATGAAAATATGCATCAACCGTTACTCAGTGAGCAGAAAATTGTGGAGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

546

Amino Acids

60.49

Weight (kDa)

7.64

Isoelectric Point (pI)

40.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 30 - 440 5.9e-53 Sodium/hydrogen exchanger, transmembrane
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1197
AasI GACNNNNNNGTC 1 cut(s) 331
Acc16I TGCGCA 1 cut(s) 1466
AccBSI CCGCTC 1 cut(s) 1396
AccII CGCG 1 cut(s) 701
AciI CCGC 9 cut(s) 263, 304, 683, 791, 837, 1293, 1331, 1394, 1548
AclI AACGTT 1 cut(s) 914
AclWI GGATC 1 cut(s) 1409
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 3 cut(s) 403, 590, 986
AfaI GTAC 4 cut(s) 570, 704, 722, 1258
AfeI AGCGCT 1 cut(s) 156
AfiI CCNNNNNNNGG 2 cut(s) 427, 1544
AgsI TTSAA 5 cut(s) 387, 577, 628, 805, 885
AhlI ACTAGT 1 cut(s) 1253
AjiI CACGTC 1 cut(s) 333
AjnI CCWGG 3 cut(s) 860, 1003, 1100
AleI CACNNNNGTG 2 cut(s) 563, 1283
AluBI AGCT 3 cut(s) 53, 213, 1064
AluI AGCT 3 cut(s) 53, 213, 1064
Alw26I GTCTC 3 cut(s) 13, 500, 1386
AlwI GGATC 1 cut(s) 1409
Aor51HI AGCGCT 1 cut(s) 156
AoxI GGCC 1 cut(s) 39
ApeKI GCWGC 3 cut(s) 680, 1061, 1571
ApoI RAATTY 3 cut(s) 403, 590, 986
AspLEI GCGC 3 cut(s) 157, 701, 1467
AsuHPI GGTGA 5 cut(s) 31, 43, 304, 1153, 1572
BanII GRGCYC 1 cut(s) 1004
BarI GAAGNNNNNNTAC 2 cut(s) 141, 173
BbvI GCAGC 3 cut(s) 692, 1073, 1558
BccI CCATC 4 cut(s) 126, 421, 1462, 1517
BcgI CGANNNNNNTGC 4 cut(s) 441, 475, 900, 934
BciT130I CCWGG 3 cut(s) 862, 1005, 1102
BciVI GTATCC 1 cut(s) 1189
BclI TGATCA 1 cut(s) 1248
BcoDI GTCTC 3 cut(s) 13, 500, 1386
BcuI ACTAGT 1 cut(s) 1253
BfaI CTAG 5 cut(s) 380, 440, 527, 1254, 1434
BfmI CTRYAG 2 cut(s) 465, 520
BfoI RGCGCY 1 cut(s) 158
BfuI GTATCC 1 cut(s) 1189
BglII AGATCT 2 cut(s) 238, 933
BisI GCNGC 7 cut(s) 263, 681, 792, 1062, 1331, 1394, 1572
BlpI GCTNAGC 1 cut(s) 695
BlsI GCNGC 7 cut(s) 264, 682, 793, 1063, 1332, 1395, 1573
Bme1390I CCNGG 3 cut(s) 862, 1005, 1102
BmgBI CACGTC 1 cut(s) 333
BmrFI CCNGG 3 cut(s) 862, 1005, 1102
BmsI GCATC 4 cut(s) 544, 615, 952, 1606
BpmI CTGGAG 1 cut(s) 140
Bpu1102I GCTNAGC 1 cut(s) 695
BpuEI CTTGAG 1 cut(s) 443
BsaBI GATNNNNATC 1 cut(s) 938
BsaI GGTCTC 1 cut(s) 13
BsaJI CCNNGG 6 cut(s) 201, 724, 966, 1003, 1004, 1182
BsaXI ACNNNNNCTCC 2 cut(s) 528, 558
Bsc4I CCNNNNNNNGG 2 cut(s) 427, 1544
Bse118I RCCGGY 1 cut(s) 1574
Bse1I ACTGG 1 cut(s) 45
Bse3DI GCAATG 2 cut(s) 618, 1215
Bse8I GATNNNNATC 1 cut(s) 938
BseBI CCWGG 3 cut(s) 862, 1005, 1102
BseDI CCNNGG 6 cut(s) 201, 724, 966, 1003, 1004, 1182
BseGI GGATG 3 cut(s) 141, 967, 1454
BseJI GATNNNNATC 1 cut(s) 938
BseLI CCNNNNNNNGG 2 cut(s) 427, 1544
BseMI GCAATG 2 cut(s) 618, 1215
BseMII CTCAG 4 cut(s) 921, 1124, 1359, 1624
BseNI ACTGG 1 cut(s) 45
BseRI GAGGAG 1 cut(s) 1388
BseXI GCAGC 3 cut(s) 692, 1073, 1558
BsgI GTGCAG 1 cut(s) 699
Bsh1236I CGCG 1 cut(s) 701
BshFI GGCC 1 cut(s) 41
BsiSI CCGG 2 cut(s) 282, 1575
BslFI GGGAC 1 cut(s) 1021
BslI CCNNNNNNNGG 2 cut(s) 427, 1544
BsmAI GTCTC 3 cut(s) 13, 500, 1386
BsmFI GGGAC 1 cut(s) 1021
BsnI GGCC 1 cut(s) 41
Bso31I GGTCTC 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 1004
Bsp143I GATC 8 cut(s) 35, 66, 238, 595, 742, 933, 1248, 1414
Bsp1720I GCTNAGC 1 cut(s) 695
BspACI CCGC 9 cut(s) 263, 304, 683, 791, 837, 1293, 1331, 1394, 1548
BspANI GGCC 1 cut(s) 41
BspCNI CTCAG 4 cut(s) 922, 1123, 1360, 1623
BspFNI CGCG 1 cut(s) 701
BspHI TCATGA 2 cut(s) 321, 1245
BspPI GGATC 1 cut(s) 1409
BspQI GCTCTTC 1 cut(s) 1051
BspTNI GGTCTC 1 cut(s) 13
BsrBI CCGCTC 1 cut(s) 1396
BsrDI GCAATG 2 cut(s) 618, 1215
BsrFI RCCGGY 1 cut(s) 1574
BsrI ACTGG 1 cut(s) 45
BssAI RCCGGY 1 cut(s) 1574
BssECI CCNNGG 6 cut(s) 201, 724, 966, 1003, 1004, 1182
BssMI GATC 8 cut(s) 35, 66, 238, 595, 742, 933, 1248, 1414
BssT1I CCWWGG 2 cut(s) 201, 966
Bst2UI CCWGG 3 cut(s) 862, 1005, 1102
Bst4CI ACNGT 5 cut(s) 524, 826, 1147, 1267, 1605
Bst6I CTCTTC 3 cut(s) 248, 1051, 1280
BstC8I GCNNGC 4 cut(s) 839, 903, 1463, 1469
BstDSI CCRYGG 1 cut(s) 1182
BstF5I GGATG 3 cut(s) 141, 967, 1454
BstFNI CGCG 1 cut(s) 701
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 3 cut(s) 157, 701, 1467
BstKTI GATC 8 cut(s) 38, 69, 241, 598, 745, 936, 1251, 1417
BstMAI GTCTC 3 cut(s) 13, 500, 1386
BstMBI GATC 8 cut(s) 35, 66, 238, 595, 742, 933, 1248, 1414
BstMWI GCNNNNNNNGC 1 cut(s) 449
BstNI CCWGG 3 cut(s) 862, 1005, 1102
BstNSI RCATGY 3 cut(s) 905, 1471, 1534
BstSCI CCNGG 3 cut(s) 860, 1003, 1100
BstSFI CTRYAG 2 cut(s) 465, 520
BstUI CGCG 1 cut(s) 701
BstV1I GCAGC 3 cut(s) 692, 1073, 1558
BstX2I RGATCY 3 cut(s) 238, 933, 1414
BstXI CCANNNNNNTGG 1 cut(s) 565
BstYI RGATCY 3 cut(s) 238, 933, 1414
BsuI GTATCC 1 cut(s) 1189
BsuRI GGCC 1 cut(s) 41
BtgI CCRYGG 1 cut(s) 1182
BtrI CACGTC 1 cut(s) 333
BtsCI GGATG 3 cut(s) 141, 967, 1454
BtsIMutI CAGTG 2 cut(s) 52, 1618
Cac8I GCNNGC 4 cut(s) 839, 903, 1463, 1469
CciI TCATGA 2 cut(s) 321, 1245
CfoI GCGC 3 cut(s) 157, 701, 1467
Cfr10I RCCGGY 1 cut(s) 1574
CseI GACGC 1 cut(s) 1523
Csp6I GTAC 4 cut(s) 569, 703, 721, 1257
CspCI CAANNNNNGTGG 2 cut(s) 793, 828
CviAII CATG 9 cut(s) 322, 667, 847, 902, 1165, 1246, 1354, 1468, 1531
CviQI GTAC 4 cut(s) 569, 703, 721, 1257
DpnI GATC 8 cut(s) 37, 68, 240, 597, 744, 935, 1250, 1416
DpnII GATC 8 cut(s) 35, 66, 238, 595, 742, 933, 1248, 1414
DraI TTTAAA 1 cut(s) 400
DrdI GACNNNNNNGTC 1 cut(s) 331
DseDI GACNNNNNNGTC 1 cut(s) 331
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 3 cut(s) 248, 1051, 1280
EarI CTCTTC 3 cut(s) 248, 1051, 1280
Eco130I CCWWGG 2 cut(s) 201, 966
Eco24I GRGCYC 1 cut(s) 1004
Eco31I GGTCTC 1 cut(s) 13
Eco47III AGCGCT 1 cut(s) 156
EcoRII CCWGG 3 cut(s) 860, 1003, 1100
EcoT14I CCWWGG 2 cut(s) 201, 966
EcoT22I ATGCAT 3 cut(s) 101, 393, 1599
EcoT38I GRGCYC 1 cut(s) 1004
ErhI CCWWGG 2 cut(s) 201, 966
FaeI CATG 9 cut(s) 325, 670, 850, 905, 1168, 1249, 1357, 1471, 1534
FalI AAGNNNNNCTT 4 cut(s) 741, 773, 1048, 1080
FaqI GGGAC 1 cut(s) 1021
FatI CATG 9 cut(s) 321, 666, 846, 901, 1164, 1245, 1353, 1467, 1530
FauI CCCGC 1 cut(s) 830
FbaI TGATCA 1 cut(s) 1248
Fnu4HI GCNGC 7 cut(s) 263, 681, 792, 1062, 1331, 1394, 1572
FokI GGATG 3 cut(s) 148, 974, 1441
FriOI GRGCYC 1 cut(s) 1004
Fsp4HI GCNGC 7 cut(s) 263, 681, 792, 1062, 1331, 1394, 1572
FspBI CTAG 5 cut(s) 380, 440, 527, 1254, 1434
FspI TGCGCA 1 cut(s) 1466
GlaI GCGC 3 cut(s) 156, 700, 1466
GluI GCNGC 7 cut(s) 263, 681, 792, 1062, 1331, 1394, 1572
GsuI CTGGAG 1 cut(s) 140
HaeII RGCGCY 1 cut(s) 158
HaeIII GGCC 1 cut(s) 41
HapII CCGG 2 cut(s) 282, 1575
HgaI GACGC 1 cut(s) 1523
HhaI GCGC 3 cut(s) 157, 701, 1467
Hin1II CATG 9 cut(s) 325, 670, 850, 905, 1168, 1249, 1357, 1471, 1534
Hin6I GCGC 3 cut(s) 155, 699, 1465
HinP1I GCGC 3 cut(s) 155, 699, 1465
HincII GTYRAC 2 cut(s) 660, 1215
HindII GTYRAC 2 cut(s) 660, 1215
HindIII AAGCTT 1 cut(s) 51
HinfI GANTC 9 cut(s) 62, 143, 470, 763, 1020, 1161, 1339, 1370, 1557
HpaII CCGG 2 cut(s) 282, 1575
HphI GGTGA 5 cut(s) 31, 43, 304, 1153, 1572
Hpy166II GTNNAC 3 cut(s) 660, 1215, 1580
Hpy188I TCNGA 7 cut(s) 449, 588, 728, 931, 1113, 1369, 1375
Hpy188III TCNNGA 9 cut(s) 119, 313, 322, 500, 746, 885, 991, 1246, 1474
Hpy8I GTNNAC 3 cut(s) 660, 1215, 1580
HpyAV CCTTC 4 cut(s) 118, 533, 723, 988
HpyCH4III ACNGT 5 cut(s) 524, 826, 1147, 1267, 1605
HpyCH4IV ACGT 3 cut(s) 332, 411, 914
HpyF10VI GCNNNNNNNGC 1 cut(s) 449
HpySE526I ACGT 3 cut(s) 332, 411, 914
Hsp92II CATG 9 cut(s) 325, 670, 850, 905, 1168, 1249, 1357, 1471, 1534
HspAI GCGC 3 cut(s) 155, 699, 1465
Ksp22I TGATCA 1 cut(s) 1248
Kzo9I GATC 8 cut(s) 35, 66, 238, 595, 742, 933, 1248, 1414
LguI GCTCTTC 1 cut(s) 1051
LmnI GCTCC 2 cut(s) 449, 1401
Lsp1109I GCAGC 3 cut(s) 692, 1073, 1558
LweI GCATC 4 cut(s) 544, 615, 952, 1606
MaeI CTAG 5 cut(s) 380, 440, 527, 1254, 1434
MaeII ACGT 3 cut(s) 332, 411, 914
MaeIII GTNAC 7 cut(s) 148, 849, 871, 889, 1141, 1526, 1605
MalI GATC 8 cut(s) 37, 68, 240, 597, 744, 935, 1250, 1416
MbiI CCGCTC 1 cut(s) 1396
MboI GATC 8 cut(s) 35, 66, 238, 595, 742, 933, 1248, 1414
MboII GAAGA 9 cut(s) 107, 235, 248, 248, 448, 928, 1068, 1267, 1424
MfeI CAATTG 1 cut(s) 1014
MflI RGATCY 3 cut(s) 238, 933, 1414
MhlI GDGCHC 1 cut(s) 1004
MlyI GAGTC 3 cut(s) 1029, 1155, 1379
MmeI TCCRAC 3 cut(s) 667, 1033, 1353
MnlI CCTC 8 cut(s) 734, 1086, 1320, 1375, 1402, 1409, 1458, 1547
Mph1103I ATGCAT 3 cut(s) 101, 393, 1599
MseI TTAA 6 cut(s) 231, 276, 399, 495, 1097, 1131
MslI CAYNNNNRTG 3 cut(s) 563, 1283, 1358
MspA1I CMGCKG 1 cut(s) 683
MspI CCGG 2 cut(s) 282, 1575
MspR9I CCNGG 3 cut(s) 862, 1005, 1102
MunI CAATTG 1 cut(s) 1014
MvaI CCWGG 3 cut(s) 862, 1005, 1102
MvnI CGCG 1 cut(s) 701
MwoI GCNNNNNNNGC 1 cut(s) 449
NdeII GATC 8 cut(s) 35, 66, 238, 595, 742, 933, 1248, 1414
NlaIII CATG 9 cut(s) 325, 670, 850, 905, 1168, 1249, 1357, 1471, 1534
NmeAIII GCCGAG 1 cut(s) 440
NmuCI GTSAC 2 cut(s) 849, 1141
NsbI TGCGCA 1 cut(s) 1466
NsiI ATGCAT 3 cut(s) 101, 393, 1599
NspI RCATGY 3 cut(s) 905, 1471, 1534
OliI CACNNNNGTG 2 cut(s) 563, 1283
PaeI GCATGC 2 cut(s) 905, 1471
PagI TCATGA 2 cut(s) 321, 1245
PasI CCCWGGG 1 cut(s) 1004
PciSI GCTCTTC 1 cut(s) 1051
PfeI GAWTC 6 cut(s) 62, 143, 470, 763, 1339, 1557
PkrI GCNGC 7 cut(s) 264, 682, 793, 1063, 1332, 1395, 1573
PleI GAGTC 3 cut(s) 1028, 1155, 1378
PpsI GAGTC 3 cut(s) 1028, 1155, 1378
PsiI TTATAA 1 cut(s) 1197
Psp1406I AACGTT 1 cut(s) 914
Psp6I CCWGG 3 cut(s) 860, 1003, 1100
PspGI CCWGG 3 cut(s) 860, 1003, 1100
PsuI RGATCY 3 cut(s) 238, 933, 1414
RsaI GTAC 4 cut(s) 570, 704, 722, 1258
RsaNI GTAC 4 cut(s) 569, 703, 721, 1257
RseI CAYNNNNRTG 3 cut(s) 563, 1283, 1358
SapI GCTCTTC 1 cut(s) 1051
SaqAI TTAA 6 cut(s) 231, 276, 399, 495, 1097, 1131
SatI GCNGC 7 cut(s) 263, 681, 792, 1062, 1331, 1394, 1572
Sau3AI GATC 8 cut(s) 35, 66, 238, 595, 742, 933, 1248, 1414
SchI GAGTC 3 cut(s) 1029, 1155, 1379
ScrFI CCNGG 3 cut(s) 862, 1005, 1102
SduI GDGCHC 1 cut(s) 1004
SfaNI GCATC 4 cut(s) 544, 615, 952, 1606
SfcI CTRYAG 2 cut(s) 465, 520
SmiMI CAYNNNNRTG 3 cut(s) 563, 1283, 1358
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
SpeI ACTAGT 1 cut(s) 1253
SphI GCATGC 2 cut(s) 905, 1471
SsiI CCGC 9 cut(s) 263, 304, 683, 791, 837, 1293, 1331, 1394, 1548
SspMI CTAG 5 cut(s) 380, 440, 527, 1254, 1434
StyD4I CCNGG 3 cut(s) 860, 1003, 1100
StyI CCWWGG 2 cut(s) 201, 966
TaaI ACNGT 5 cut(s) 524, 826, 1147, 1267, 1605
TaiI ACGT 3 cut(s) 335, 414, 917
TaqI TCGA 4 cut(s) 65, 130, 737, 1028
TauI GCSGC 4 cut(s) 265, 794, 1333, 1396
TfiI GAWTC 6 cut(s) 62, 143, 470, 763, 1339, 1557
Tru1I TTAA 6 cut(s) 231, 276, 399, 495, 1097, 1131
Tru9I TTAA 6 cut(s) 231, 276, 399, 495, 1097, 1131
TscAI CASTG 2 cut(s) 52, 1618
TseFI GTSAC 2 cut(s) 849, 1141
TseI GCWGC 3 cut(s) 680, 1061, 1571
Tsp45I GTSAC 2 cut(s) 849, 1141
TspDTI ATGAA 4 cut(s) 249, 310, 928, 1602
TspGWI ACGGA 3 cut(s) 46, 155, 1171
TspRI CASTG 2 cut(s) 52, 1618
XapI RAATTY 3 cut(s) 403, 590, 986
XceI RCATGY 3 cut(s) 905, 1471, 1534
XspI CTAG 5 cut(s) 380, 440, 527, 1254, 1434
Zsp2I ATGCAT 3 cut(s) 101, 393, 1599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.