pycom03g22070

Sodium hydrogen exchanger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007582
Physical Location & Seq
Reverse (-)
22260005 .. 22262739
2735 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g22070.1

Sequence Viewer

Length: 1146 bp
ATGGAGGTCTCTAGCTTGGTGAGATTGGGGGTGAGATCGGCCACCAGTGAAGCTTCCGTAGATTCGATCACTTTGTTCGTGGTGCTCCTATGTTTATGCATTGTGATTGGTCATCTTCTGGAGAAGGCTCGATGGATGACCGAATCAGTAACAGCGCTTCTCATTGGTCTTTGTACCGGAATTGTTATTCTACTAACTACCAAAGGGAAAAGCTCACACATCTTAGTATTTAATGAAGATCTCTTCTTTATATATCTTCTGCCACCTATTATATTTAACGCGGGATTTCAGGTGAAGAAGAAGCGGTTTTTCCAGAACTTCATGACAATCACGTCATTGGGTGCTGTCGGCAGTTTAGTATCATTCGTAATAATATGGCTAGGTTCAATGCATTTGTTTGAAAAATTGGACGTTGGTTTCCTTGAGATGGGGGATTATCTAGCACTCGGAGCAATCTTCTCAGCTACAGATTCAGTTTGCACCCTGCAAGTGCTTAATCAAGATGAGACACCTTTACTCTACAGTCTAGTTTTTGGAGAAGGTGTTGTAAATGATGCCACATCTGTGGTACTTTTCAACGCAATTCAGAAATTTGATCTCTCTCACATCAACTCAACCATTGCCTTGAAGTTTAGTGGCAATTTCTTATTTCTGTTGTTGACAAGCATGGTGCTTGGTGCAACGGTTGGATTGCTTAGCGCATACATCATAAAGAAGTTGTACCTCGGCAGGCACTCGACTGATCGTGAAGTTGCACTTATGATTCTAATGGCTTATTTGTCATATATGGCGGCAGAACTATTCAATCTAAGTGGTATTCTTACCGTTTTCTTTTGCGGGCTTGTCATGTCACACTATACCTGGCATAATGTAACTGAAAGTTCAAGAGTTACAACCAAGCATGCTTTTGCAGCGTTCTCGTTTGTTTCTGAGATCTTCATCTTTCTGTATGTTGGTATGGATGCCTTGGACATTGAGAAGTGGAAATTCGTAAAGGAGAGCGCTGGGACATCAATTGGAGTCAGTTCGATATTACTTGGCCTGGTTCTTGTTGGAAGAGCGGCTTTTGTATTTCCTCTATGTTTCTTATCAAATTTAACCAGGATCGATCTCAGATCGACAAAATCGGGTTTAAGCAACAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

382

Amino Acids

41.95

Weight (kDa)

7.07

Isoelectric Point (pI)

34.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 30 - 370 6e-43 Sodium/hydrogen exchanger, transmembrane
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 331
AccBSI CCGCTC 1 cut(s) 1061
AccII CGCG 1 cut(s) 281
AciI CCGC 5 cut(s) 281, 304, 791, 837, 1061
AclWI GGATC 1 cut(s) 1112
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 3 cut(s) 590, 986, 1093
AfaI GTAC 3 cut(s) 175, 570, 722
AfeI AGCGCT 2 cut(s) 156, 1003
AfiI CCNNNNNNNGG 1 cut(s) 427
AgsI TTSAA 6 cut(s) 387, 401, 577, 628, 805, 885
AjiI CACGTC 1 cut(s) 333
AjnI CCWGG 3 cut(s) 860, 1041, 1100
AleI CACNNNNGTG 1 cut(s) 563
AluBI AGCT 4 cut(s) 15, 53, 213, 464
AluI AGCT 4 cut(s) 15, 53, 213, 464
Alw21I GWGCWC 1 cut(s) 87
Alw26I GTCTC 2 cut(s) 13, 500
AlwI GGATC 1 cut(s) 1112
Aor51HI AGCGCT 2 cut(s) 156, 1003
AoxI GGCC 2 cut(s) 39, 1039
ApeKI GCWGC 1 cut(s) 911
ApoI RAATTY 3 cut(s) 590, 986, 1093
AspLEI GCGC 3 cut(s) 157, 701, 1004
AsuHPI GGTGA 3 cut(s) 31, 43, 304
BarI GAAGNNNNNNTAC 2 cut(s) 141, 173
Bbv12I GWGCWC 1 cut(s) 87
BbvI GCAGC 1 cut(s) 923
BccI CCATC 2 cut(s) 126, 421
BcgI CGANNNNNNTGC 2 cut(s) 900, 934
BciT130I CCWGG 3 cut(s) 862, 1043, 1102
BcoDI GTCTC 2 cut(s) 13, 500
BfaI CTAG 4 cut(s) 12, 380, 440, 527
BfmI CTRYAG 2 cut(s) 465, 520
BfoI RGCGCY 2 cut(s) 158, 1005
BglII AGATCT 2 cut(s) 238, 933
BisI GCNGC 3 cut(s) 792, 912, 1062
BlpI GCTNAGC 1 cut(s) 695
BlsI GCNGC 3 cut(s) 793, 913, 1063
Bme1390I CCNGG 3 cut(s) 862, 1043, 1102
BmgBI CACGTC 1 cut(s) 333
BmrFI CCNGG 3 cut(s) 862, 1043, 1102
BmsI GCATC 2 cut(s) 544, 952
BpmI CTGGAG 1 cut(s) 140
Bpu1102I GCTNAGC 1 cut(s) 695
BpuEI CTTGAG 1 cut(s) 443
Bsa29I ATCGAT 1 cut(s) 1107
BsaBI GATNNNNATC 1 cut(s) 938
BsaI GGTCTC 1 cut(s) 13
BsaJI CCNNGG 2 cut(s) 724, 966
BsaWI WCCGGW 1 cut(s) 176
BsaXI ACNNNNNCTCC 2 cut(s) 528, 558
Bsc4I CCNNNNNNNGG 1 cut(s) 427
Bse1I ACTGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 618
Bse8I GATNNNNATC 1 cut(s) 938
BseBI CCWGG 3 cut(s) 862, 1043, 1102
BseCI ATCGAT 1 cut(s) 1107
BseDI CCNNGG 2 cut(s) 724, 966
BseGI GGATG 2 cut(s) 141, 967
BseJI GATNNNNATC 1 cut(s) 938
BseLI CCNNNNNNNGG 1 cut(s) 427
BseMI GCAATG 1 cut(s) 618
BseMII CTCAG 3 cut(s) 474, 921, 1126
BseNI ACTGG 1 cut(s) 45
BseXI GCAGC 1 cut(s) 923
BseYI CCCAGC 1 cut(s) 1004
Bsh1236I CGCG 1 cut(s) 281
BshFI GGCC 2 cut(s) 41, 1041
BshVI ATCGAT 1 cut(s) 1107
BsiHKAI GWGCWC 1 cut(s) 87
BsiSI CCGG 1 cut(s) 177
BslFI GGGAC 1 cut(s) 1021
BslI CCNNNNNNNGG 1 cut(s) 427
BsmAI GTCTC 2 cut(s) 13, 500
BsmFI GGGAC 1 cut(s) 1021
BsnI GGCC 2 cut(s) 41, 1041
Bso31I GGTCTC 1 cut(s) 13
Bsp1286I GDGCHC 1 cut(s) 87
Bsp143I GATC 9 cut(s) 35, 66, 238, 595, 742, 933, 1104, 1108, 1115
Bsp1720I GCTNAGC 1 cut(s) 695
BspACI CCGC 5 cut(s) 281, 304, 791, 837, 1061
BspANI GGCC 2 cut(s) 41, 1041
BspCNI CTCAG 3 cut(s) 473, 922, 1125
BspDI ATCGAT 1 cut(s) 1107
BspFNI CGCG 1 cut(s) 281
BspHI TCATGA 1 cut(s) 321
BspPI GGATC 1 cut(s) 1112
BspQI GCTCTTC 1 cut(s) 1051
BspTNI GGTCTC 1 cut(s) 13
BsrBI CCGCTC 1 cut(s) 1061
BsrDI GCAATG 1 cut(s) 618
BsrI ACTGG 1 cut(s) 45
BssECI CCNNGG 2 cut(s) 724, 966
BssMI GATC 9 cut(s) 35, 66, 238, 595, 742, 933, 1104, 1108, 1115
BssT1I CCWWGG 1 cut(s) 966
Bst2UI CCWGG 3 cut(s) 862, 1043, 1102
Bst4CI ACNGT 3 cut(s) 524, 685, 826
Bst6I CTCTTC 2 cut(s) 248, 1051
BstC8I GCNNGC 3 cut(s) 731, 839, 903
BstDEI CTNAG 6 cut(s) 223, 460, 695, 809, 930, 1112
BstF5I GGATG 2 cut(s) 141, 967
BstFNI CGCG 1 cut(s) 281
BstH2I RGCGCY 2 cut(s) 158, 1005
BstHHI GCGC 3 cut(s) 157, 701, 1004
BstKTI GATC 9 cut(s) 38, 69, 241, 598, 745, 936, 1107, 1111, 1118
BstMAI GTCTC 2 cut(s) 13, 500
BstMBI GATC 9 cut(s) 35, 66, 238, 595, 742, 933, 1104, 1108, 1115
BstMWI GCNNNNNNNGC 2 cut(s) 449, 911
BstNI CCWGG 3 cut(s) 862, 1043, 1102
BstNSI RCATGY 1 cut(s) 905
BstSCI CCNGG 3 cut(s) 860, 1041, 1100
BstSFI CTRYAG 2 cut(s) 465, 520
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 1 cut(s) 923
BstX2I RGATCY 2 cut(s) 238, 933
BstXI CCANNNNNNTGG 1 cut(s) 565
BstYI RGATCY 2 cut(s) 238, 933
Bsu15I ATCGAT 1 cut(s) 1107
BsuRI GGCC 2 cut(s) 41, 1041
BsuTUI ATCGAT 1 cut(s) 1107
BtrI CACGTC 1 cut(s) 333
BtsCI GGATG 2 cut(s) 141, 967
BtsIMutI CAGTG 1 cut(s) 52
Cac8I GCNNGC 3 cut(s) 731, 839, 903
CciI TCATGA 1 cut(s) 321
CfoI GCGC 3 cut(s) 157, 701, 1004
ClaI ATCGAT 1 cut(s) 1107
Csp6I GTAC 3 cut(s) 174, 569, 721
CspCI CAANNNNNGTGG 2 cut(s) 793, 828
CviAII CATG 4 cut(s) 322, 667, 847, 902
CviQI GTAC 3 cut(s) 174, 569, 721
DdeI CTNAG 6 cut(s) 223, 460, 695, 809, 930, 1112
DpnI GATC 9 cut(s) 37, 68, 240, 597, 744, 935, 1106, 1110, 1117
DpnII GATC 9 cut(s) 35, 66, 238, 595, 742, 933, 1104, 1108, 1115
DrdI GACNNNNNNGTC 1 cut(s) 331
DseDI GACNNNNNNGTC 1 cut(s) 331
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 2 cut(s) 248, 1051
EarI CTCTTC 2 cut(s) 248, 1051
Eco130I CCWWGG 1 cut(s) 966
Eco31I GGTCTC 1 cut(s) 13
Eco47III AGCGCT 2 cut(s) 156, 1003
EcoRII CCWGG 3 cut(s) 860, 1041, 1100
EcoT14I CCWWGG 1 cut(s) 966
EcoT22I ATGCAT 2 cut(s) 101, 393
ErhI CCWWGG 1 cut(s) 966
FaeI CATG 4 cut(s) 325, 670, 850, 905
FalI AAGNNNNNCTT 4 cut(s) 741, 773, 1048, 1080
FaqI GGGAC 1 cut(s) 1021
FatI CATG 4 cut(s) 321, 666, 846, 901
FauI CCCGC 2 cut(s) 274, 830
Fnu4HI GCNGC 3 cut(s) 792, 912, 1062
FokI GGATG 2 cut(s) 148, 974
Fsp4HI GCNGC 3 cut(s) 792, 912, 1062
FspBI CTAG 4 cut(s) 12, 380, 440, 527
GlaI GCGC 3 cut(s) 156, 700, 1003
GluI GCNGC 3 cut(s) 792, 912, 1062
GsaI CCCAGC 1 cut(s) 1008
GsuI CTGGAG 1 cut(s) 140
HaeII RGCGCY 2 cut(s) 158, 1005
HaeIII GGCC 2 cut(s) 41, 1041
HapII CCGG 1 cut(s) 177
HhaI GCGC 3 cut(s) 157, 701, 1004
Hin1II CATG 4 cut(s) 325, 670, 850, 905
Hin6I GCGC 3 cut(s) 155, 699, 1002
HinP1I GCGC 3 cut(s) 155, 699, 1002
HincII GTYRAC 1 cut(s) 660
HindII GTYRAC 1 cut(s) 660
HindIII AAGCTT 1 cut(s) 51
HinfI GANTC 5 cut(s) 62, 143, 470, 763, 1020
HpaII CCGG 1 cut(s) 177
HphI GGTGA 3 cut(s) 31, 43, 304
Hpy166II GTNNAC 1 cut(s) 660
Hpy188I TCNGA 4 cut(s) 449, 588, 931, 1115
Hpy188III TCNNGA 6 cut(s) 119, 313, 322, 500, 746, 885
Hpy8I GTNNAC 1 cut(s) 660
HpyAV CCTTC 2 cut(s) 118, 533
HpyCH4III ACNGT 3 cut(s) 524, 685, 826
HpyCH4IV ACGT 2 cut(s) 332, 411
HpyCH4V TGCA 7 cut(s) 99, 391, 480, 487, 680, 755, 911
HpyF10VI GCNNNNNNNGC 2 cut(s) 449, 911
HpyF3I CTNAG 6 cut(s) 223, 460, 695, 809, 930, 1112
HpySE526I ACGT 2 cut(s) 332, 411
Hsp92II CATG 4 cut(s) 325, 670, 850, 905
HspAI GCGC 3 cut(s) 155, 699, 1002
Kzo9I GATC 9 cut(s) 35, 66, 238, 595, 742, 933, 1104, 1108, 1115
LguI GCTCTTC 1 cut(s) 1051
LmnI GCTCC 2 cut(s) 90, 449
Lsp1109I GCAGC 1 cut(s) 923
LweI GCATC 2 cut(s) 544, 952
MaeI CTAG 4 cut(s) 12, 380, 440, 527
MaeII ACGT 2 cut(s) 332, 411
MaeIII GTNAC 4 cut(s) 148, 849, 871, 889
MalI GATC 9 cut(s) 37, 68, 240, 597, 744, 935, 1106, 1110, 1117
MbiI CCGCTC 1 cut(s) 1061
MboI GATC 9 cut(s) 35, 66, 238, 595, 742, 933, 1104, 1108, 1115
MboII GAAGA 9 cut(s) 107, 235, 248, 248, 307, 310, 448, 928, 1068
MfeI CAATTG 1 cut(s) 1014
MflI RGATCY 2 cut(s) 238, 933
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 8 cut(s) 180, 404, 582, 590, 640, 986, 1014, 1093
MlyI GAGTC 1 cut(s) 1029
MmeI TCCRAC 2 cut(s) 667, 1033
MnlI CCTC 2 cut(s) 734, 1086
Mph1103I ATGCAT 2 cut(s) 101, 393
MseI TTAA 5 cut(s) 231, 276, 495, 1097, 1133
MslI CAYNNNNRTG 1 cut(s) 563
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 3 cut(s) 862, 1043, 1102
MunI CAATTG 1 cut(s) 1014
MvaI CCWGG 3 cut(s) 862, 1043, 1102
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 2 cut(s) 449, 911
NdeII GATC 9 cut(s) 35, 66, 238, 595, 742, 933, 1104, 1108, 1115
NlaIII CATG 4 cut(s) 325, 670, 850, 905
NmeAIII GCCGAG 1 cut(s) 705
NmuCI GTSAC 1 cut(s) 849
NsiI ATGCAT 2 cut(s) 101, 393
NspI RCATGY 1 cut(s) 905
OliI CACNNNNGTG 1 cut(s) 563
PaeI GCATGC 1 cut(s) 905
PagI TCATGA 1 cut(s) 321
PciSI GCTCTTC 1 cut(s) 1051
PfeI GAWTC 4 cut(s) 62, 143, 470, 763
PkrI GCNGC 3 cut(s) 793, 913, 1063
PleI GAGTC 1 cut(s) 1028
PpsI GAGTC 1 cut(s) 1028
Psp6I CCWGG 3 cut(s) 860, 1041, 1100
PspFI CCCAGC 1 cut(s) 1004
PspGI CCWGG 3 cut(s) 860, 1041, 1100
PsuI RGATCY 2 cut(s) 238, 933
RsaI GTAC 3 cut(s) 175, 570, 722
RsaNI GTAC 3 cut(s) 174, 569, 721
RseI CAYNNNNRTG 1 cut(s) 563
SapI GCTCTTC 1 cut(s) 1051
SaqAI TTAA 5 cut(s) 231, 276, 495, 1097, 1133
SatI GCNGC 3 cut(s) 792, 912, 1062
Sau3AI GATC 9 cut(s) 35, 66, 238, 595, 742, 933, 1104, 1108, 1115
SchI GAGTC 1 cut(s) 1029
ScrFI CCNGG 3 cut(s) 862, 1043, 1102
SduI GDGCHC 1 cut(s) 87
SfaNI GCATC 2 cut(s) 544, 952
SfcI CTRYAG 2 cut(s) 465, 520
SmiMI CAYNNNNRTG 1 cut(s) 563
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
SphI GCATGC 1 cut(s) 905
Sse9I AATT 8 cut(s) 180, 404, 582, 590, 640, 986, 1014, 1093
SsiI CCGC 5 cut(s) 281, 304, 791, 837, 1061
SspMI CTAG 4 cut(s) 12, 380, 440, 527
StyD4I CCNGG 3 cut(s) 860, 1041, 1100
StyI CCWWGG 1 cut(s) 966
TaaI ACNGT 3 cut(s) 524, 685, 826
TaiI ACGT 2 cut(s) 335, 414
TaqI TCGA 6 cut(s) 65, 130, 737, 1028, 1107, 1118
TaqII GACCGA 1 cut(s) 155
TasI AATT 8 cut(s) 180, 404, 582, 590, 640, 986, 1014, 1093
TauI GCSGC 2 cut(s) 794, 1064
TfiI GAWTC 4 cut(s) 62, 143, 470, 763
Tru1I TTAA 5 cut(s) 231, 276, 495, 1097, 1133
Tru9I TTAA 5 cut(s) 231, 276, 495, 1097, 1133
TscAI CASTG 1 cut(s) 52
TseFI GTSAC 1 cut(s) 849
TseI GCWGC 1 cut(s) 911
Tsp45I GTSAC 1 cut(s) 849
TspDTI ATGAA 3 cut(s) 249, 310, 928
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 1 cut(s) 52
XapI RAATTY 3 cut(s) 590, 986, 1093
XceI RCATGY 1 cut(s) 905
XspI CTAG 4 cut(s) 12, 380, 440, 527
Zsp2I ATGCAT 2 cut(s) 101, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.