Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003412

Physical Location & Seq

Genomic Coordinates Forward (+)

2711302 .. 2721667

Gene Structure 10366 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1023600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2589 bp

ATGGCTTCCAACAAAGTTTTAATGGTCGCCGAGAAGCCGAGCATAGCTCTTTCCATAGCCTCCGTTCTCTCCCATGGCCAGATGTCTACAAGAAGGGGTAGTACGGAAGTCCATGAATTTGAAGGAAAGTTTCTTGGAGTGCATGTGCATTTTAAAGTGACATCAGTTATTGGTCATGTGTTCAGTGTAGATTTTCCAGAAAAATATCAAAATTGGACATTGACTGATCCTCTGGATCTTTTTCAAGCTCCAGTTCTCAAGACAGAAGCAAATCCAAAGGCTCACATTCGCAGACATTTAAGCCAAGAAGCTCGCGGTTATGGTAACTTGGTTTTGTGGTTGGACTGTGATCGTGAAGGGGAAAACATTTGCTTTGAAGTTATCGAGTGTATTGGCCATTCAAATGAGACTAGAAGAAAGATCTATCGTGCTCGGTTTTCCTCTGTTACTGAGAAAGATATTTTGAAGGCCATGGACAACCTTGTTGAACCTAATAAAGACGAGGCATTGTCAGTAGATGCTCGGCAAGAGATAGATTTGAAAGTTGGAGTTGCATTTACACGATTTCAAACACACTTTTTCCAGGGGAAATACGGAAATCTTGATGCCAGAGTTATCTCCTATGGCCCTTGCCAAACTCCGACCCTTGGATTTTGCGTACAGCGGTATTTGCAGATAAATACATTTAAACCAGAAAAGTTCTGGGTCGTACGCCCATACATAATACAGAAGGGCTACGAACTTCAGCTAGAATGGGAACGTAACAAGTTGTTTGACTTCGACGTTGCTGTAATGTTTCAAAAATTAGTAATGGAAGATGGAATGCTAGTAGTGACTGAAATAATAGAAAAGCAGGAAAGCAAAAGTCGTCCATCTGGGCTTAATACGGTCAATCTTCTGAAGGTTGCCTCAAGTGCACTAGGCTTTGGACCACAAATGGCTATGCAAGTTGCTGAACGCTTGTATACTCAAGGTTTCATCAGCTATCCGCGTACCGAGAGCACAGCTTATCCATCTTCTTTTGACTTTAGAGGCACACTTGGTGCACAAGTAAATAACCCAACATGGGGTGATTATGTACAGAGGCTGCTTGCTGATGGTTATCAGAAGCCACGATCAGGGACAGATGTAGGTGACCATCCTCCTGTCACTCCGTTGAGGTCTGCAACTGAGGACATGCTTGGAAATGATGCTTGGAGACTATATGAGTACATTTGTAAACACTTCATTGGGACTGTATCTCCTGACTGCAAATATGTAAGAACAAAGGTAGATTTTTCTATTGGTGGAGAATCCTTCCATTGTGTCGGGCAACGTGTTTTGGTGAAAGGATTTACTTCTATCATGCCATGGTTGGCAATAAATGAGAAAAATCTTCCTCAGTTTACAAAAGGGGAGAAATTAGAAGTTTCAACAGTGGACCTTCATGAGGGGTTGACTTCACCTCCAGATTATCTTAGCGAGAGTGAGCTAATTTCCCTTATGGAGAAAAATGGAATTGGCACGGATGCTTCAATATCTGTTCATATTAACAACATATGCGAGCGCAATTATGTGCAGGTACAAGCTGGCAGGAAGTTGGTTCCGACTGCCTTGGGTATTACTCTAATAAGAGGCTATCAATGCATTGATCCAGATCTCTGTTTGCCAGATATTCGCAGTTTCATTGAGCAACAGATTACTCTTGTTGCTAAGGGTCAAGCAGATCATTTTCGTGTTGTGCAGCATGTCCTGCAACAATTCATGCAAAAATACAGTTATTTTGTCAAGCAGATTGAAAACATGGATGCATTATTTGAAGCACAATTCTCTCCCCTTGCTGACTCAGGACGAGTTCTCAGTAAATGTGGAAAATGTCTACGGTACATGAAGCATATTTCCAGCCAACCTTCGCGGTTGTATTGTAATACATGCGAGGAGGTTTATTATCTTCCTCAAAAGGGTAGCATAAAGCTCTACAAGGAAATTACTTGTCCTCTGGACAACTTTGAGCTATTAATCTGCTCCATGCCCGGCCCAGATGGCAAGTCATTCTCTCTCTGCCCATACTGCTACAATAGTCCTCCATTTGAAGGTATCGACACGCTCTTTGGTGCTGTAAAATCTGGCCCGTCTGGCAAGCTGGGAAAGGGAGCCGGCATGCCATGCTTCCTCTGCCCACACCCCACATGTCGGCATTCTTTGATAGCCCAAGGAGTCTGTGCTTGCCCGGACTGTAGTGGAACACTTGTTCTAGACCCTGTGAGTGCTCCCAAGTGGCGGCTCTGCTGCAACATGTGCAATTTCCTTGTCTTCCTACCCCAAGGTGCTCACCAAATTTCCACTACTCGAGAGCGGTGTCCAGAGTGCGACTCCACAATCTTAGAAGTGGATTTCAATAAAAAGACGACACCCTTGGAGAACGGAGAGACCTTGTACACTGGTTGCATTCTTTGTGATGAATTGCTCCATTCACTAGTGGAGATGAAATATGGCAAATCGTTTTTCGGATCAAGAGGAAGGGGCAGAGGAAGGAGAGGTGGACATAGGGGAAGAGGGCGAGGTGCGGGTAAGAAGGTGGATCCCAGAATGAGCTTCCGGGATTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000793 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0003916 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006265 GO:0006725 GO:0006807 GO:0006996 GO:0007059 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016853 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0051276 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140097 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03440 ko03460 map03440 map03460

Pfam Domains

Protein Families

Topoisom_bac Toprim

Protein Analysis

863

Amino Acids

96.86

Weight (kDa)

8.07

Isoelectric Point (pI)

33.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Toprim	PF01751	6 - 148	1.1e-13	Toprim domain
Topoisom_bac	PF01131	164 - 574	6.4e-106	DNA topoisomerase
Zn_ribbon_TOP3B	PF23546	608 - 647	5.3e-20	DNA topoisomerase 3-beta zinc ribbon domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003412)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G32000 AT2G32000
fragaria_vesca	FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750
malus_domestica	MD04G1023600.v1.1
prunus_persica	Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1
pyrus_communis	pycom04g02020
rosa_chinensis	RchiOBHm_Chr4g0412031
rosa_laevigata	RLG00000008327
rosa_multiflora	Rmu_co8041726.1_g000001 Rmu_sc0000711.1_g000051
rosa_roxburghii	Rroxscaffold_1G00042480 Rroxscaffold_5G00346360 Rroxscaffold_5G00356250 Rroxscaffold_5G00356340
rosa_rugosa	Rorug04G0106700 Rorug04G0106800 Rorug04G0106900
rosa_samantha	Rh4AG171100 Rh4BG168800 Rh4BG169700 Rh4CG181900 Rh4DG164200 Rh4DG165300
rosa_wichuraiana	Rw0G007770 Rw0G008800 Rw4G014260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1549
AccBSI	CCGCTC	1 cut(s)	2335
AccI	GTMKAC	3 cut(s)	86, 965, 1858
AccII	CGCG	3 cut(s)	315, 991, 1894
AciI	CCGC	7 cut(s)	315, 664, 989, 1894, 2260, 2335, 2546
AclWI	GGATC	6 cut(s)	221, 243, 1625, 2497, 2555, 2568
AcoI	YGGCCR	2 cut(s)	76, 394
AcsI	RAATTY	2 cut(s)	116, 2316
AcuI	CTGAAG	2 cut(s)	728, 920
AdeI	CACNNNGTG	1 cut(s)	1043
AfaI	GTAC	9 cut(s)	103, 660, 711, 994, 1080, 1211, 1563, 1865, 2417
AfiI	CCNNNNNNNGG	9 cut(s)	647, 1066, 1067, 1118, 1429, 1940, 2072, 2172, 2259
AflIII	ACRYGT	3 cut(s)	1315, 2168, 2274
AhlI	ACTAGT	1 cut(s)	2455
AjnI	CCWGG	1 cut(s)	582
AjuI	GAANNNNNNNTTGG	2 cut(s)	1177, 1209
AloI	GAACNNNNNNTCC	2 cut(s)	2214, 2246
Alw21I	GWGCWC	6 cut(s)	433, 919, 1004, 1048, 2251, 2311
Alw26I	GTCTC	3 cut(s)	401, 1192, 2402
Alw44I	GTGCAC	2 cut(s)	915, 1044
AlwI	GGATC	6 cut(s)	221, 243, 1625, 2497, 2555, 2568
AlwNI	CAGNNNCTG	1 cut(s)	1087
Ama87I	CYCGRG	1 cut(s)	2328
AoxI	GGCC	6 cut(s)	76, 394, 468, 625, 2014, 2107
ApaLI	GTGCAC	2 cut(s)	915, 1044
ApeKI	GCWGC	3 cut(s)	1087, 1723, 2268
ApoI	RAATTY	2 cut(s)	116, 2316
ArsI	GACNNNNNNTTYG	2 cut(s)	2072, 2104
AseI	ATTAAT	1 cut(s)	1997
AspLEI	GCGC	1 cut(s)	1548
AspS9I	GGNCC	5 cut(s)	626, 929, 1420, 2015, 2108
AsuC2I	CCSGG	3 cut(s)	2013, 2210, 2579
AsuHPI	GGTGA	5 cut(s)	1082, 1145, 1336, 1434, 2303
AvaI	CYCGRG	1 cut(s)	2328
AvaII	GGWCC	2 cut(s)	929, 1420
BaeGI	GKGCMC	2 cut(s)	919, 1048
BalI	TGGCCA	2 cut(s)	78, 396
BamHI	GGATCC	1 cut(s)	2560
BarI	GAAGNNNNNNTAC	2 cut(s)	85, 117
BbsI	GAAGAC	1 cut(s)	2284
Bbv12I	GWGCWC	6 cut(s)	433, 919, 1004, 1048, 2251, 2311
BbvI	GCAGC	3 cut(s)	1074, 1735, 2255
BccI	CCATC	6 cut(s)	812, 880, 1021, 1091, 1146, 2015
BciT130I	CCWGG	1 cut(s)	584
BcnI	CCSGG	3 cut(s)	2013, 2210, 2579
BcoDI	GTCTC	3 cut(s)	401, 1192, 2402
BcuI	ACTAGT	1 cut(s)	2455
BfaI	CTAG	6 cut(s)	411, 749, 827, 920, 2234, 2456
BfmI	CTRYAG	1 cut(s)	2215
BfuAI	ACCTGC	1 cut(s)	1549
BglI	GCCNNNNNGGC	2 cut(s)	2022, 2115
BglII	AGATCT	2 cut(s)	420, 1636
BisI	GCNGC	4 cut(s)	1088, 1724, 2261, 2269
BlsI	GCNGC	4 cut(s)	1089, 1725, 2262, 2270
Bme1390I	CCNGG	4 cut(s)	584, 2013, 2210, 2579
Bme18I	GGWCC	2 cut(s)	929, 1420
BmeT110I	CYCGRG	1 cut(s)	2328
BmgT120I	GGNCC	5 cut(s)	626, 929, 1420, 2015, 2108
BmiI	GGNNCC	3 cut(s)	1584, 2134, 2562
BmrFI	CCNGG	4 cut(s)	584, 2013, 2210, 2579
BmsI	GCATC	5 cut(s)	508, 595, 1180, 1498, 1777
BpiI	GAAGAC	1 cut(s)	2284
BplI	GAGNNNNNCTC	4 cut(s)	31, 63, 2336, 2368
BpmI	CTGGAG	2 cut(s)	234, 1431
Bpu10I	CCTNAGC	1 cut(s)	1692
BpuEI	CTTGAG	3 cut(s)	242, 895, 954
BpuMI	CCSGG	3 cut(s)	2013, 2210, 2579
BsaBI	GATNNNNATC	2 cut(s)	1101, 1635
BsaI	GGTCTC	1 cut(s)	2402
BsaJI	CCNNGG	9 cut(s)	73, 471, 583, 646, 1349, 1593, 2191, 2302, 2394
BsaXI	ACNNNNNCTCC	6 cut(s)	129, 159, 1225, 1255, 1429, 1459
Bsc4I	CCNNNNNNNGG	9 cut(s)	647, 1066, 1067, 1118, 1429, 1940, 2072, 2172, 2259
Bse118I	RCCGGY	1 cut(s)	2135
Bse1I	ACTGG	2 cut(s)	251, 2425
Bse8I	GATNNNNATC	2 cut(s)	1101, 1635
BseBI	CCWGG	1 cut(s)	584
BseDI	CCNNGG	9 cut(s)	73, 471, 583, 646, 1349, 1593, 2191, 2302, 2394
BseGI	GGATG	3 cut(s)	1138, 1513, 1792
BseJI	GATNNNNATC	2 cut(s)	1101, 1635
BseLI	CCNNNNNNNGG	9 cut(s)	647, 1066, 1067, 1118, 1429, 1940, 2072, 2172, 2259
BseMII	CTCAG	5 cut(s)	441, 1161, 1394, 1839, 1852
BseNI	ACTGG	2 cut(s)	251, 2425
BseRI	GAGGAG	1 cut(s)	1931
BseSI	GKGCMC	2 cut(s)	919, 1048
BseXI	GCAGC	3 cut(s)	1074, 1735, 2255
BseYI	CCCAGC	1 cut(s)	2122
BsgI	GTGCAG	2 cut(s)	1577, 1742
Bsh1236I	CGCG	3 cut(s)	315, 991, 1894
BshFI	GGCC	6 cut(s)	78, 396, 470, 627, 2016, 2109
BsiHKAI	GWGCWC	6 cut(s)	433, 919, 1004, 1048, 2251, 2311
BsiHKCI	CYCGRG	1 cut(s)	2328
BsiSI	CCGG	4 cut(s)	2013, 2136, 2210, 2578
BsiWI	CGTACG	1 cut(s)	709
BslFI	GGGAC	2 cut(s)	1135, 1246
BslI	CCNNNNNNNGG	9 cut(s)	647, 1066, 1067, 1118, 1429, 1940, 2072, 2172, 2259
BsmAI	GTCTC	3 cut(s)	401, 1192, 2402
BsmFI	GGGAC	2 cut(s)	1135, 1246
BsmI	GAATGC	3 cut(s)	828, 2176, 2427
BsnI	GGCC	6 cut(s)	78, 396, 470, 627, 2016, 2109
Bso31I	GGTCTC	1 cut(s)	2402
BsoBI	CYCGRG	1 cut(s)	2328
Bsp1286I	GDGCHC	6 cut(s)	433, 919, 1004, 1048, 2251, 2311
Bsp1407I	TGTACA	2 cut(s)	1078, 2415
Bsp19I	CCATGG	3 cut(s)	73, 471, 1349
BspACI	CCGC	7 cut(s)	315, 664, 989, 1894, 2260, 2335, 2546
BspANI	GGCC	6 cut(s)	78, 396, 470, 627, 2016, 2109
BspCNI	CTCAG	5 cut(s)	442, 1162, 1393, 1838, 1851
BspFNI	CGCG	3 cut(s)	315, 991, 1894
BspHI	TCATGA	1 cut(s)	1426
BspLI	GGNNCC	3 cut(s)	1584, 2134, 2562
BspMI	ACCTGC	1 cut(s)	1549
BspPI	GGATC	6 cut(s)	221, 243, 1625, 2497, 2555, 2568
BspTNI	GGTCTC	1 cut(s)	2402
BsrBI	CCGCTC	1 cut(s)	2335
BsrFI	RCCGGY	1 cut(s)	2135
BsrGI	TGTACA	2 cut(s)	1078, 2415
BsrI	ACTGG	2 cut(s)	251, 2425
BssAI	RCCGGY	1 cut(s)	2135
BssECI	CCNNGG	9 cut(s)	73, 471, 583, 646, 1349, 1593, 2191, 2302, 2394
BssNAI	GTATAC	1 cut(s)	966
BssT1I	CCWWGG	8 cut(s)	73, 471, 646, 1349, 1593, 2191, 2302, 2394
Bst1107I	GTATAC	1 cut(s)	966
Bst2UI	CCWGG	1 cut(s)	584
Bst4CI	ACNGT	7 cut(s)	347, 889, 1237, 1417, 1757, 1863, 2216
Bst6I	CTCTTC	1 cut(s)	2527
BstAPI	GCANNNNNTGC	3 cut(s)	1732, 2145, 2277
BstAUI	TGTACA	2 cut(s)	1078, 2415
BstC8I	GCNNGC	8 cut(s)	313, 1092, 1544, 1570, 2120, 2137, 2141, 2206
BstDEI	CTNAG	8 cut(s)	450, 1170, 1380, 1457, 1692, 1825, 1838, 2362
BstDSI	CCRYGG	3 cut(s)	73, 471, 1349
BstEII	GGTNACC	1 cut(s)	1133
BstENI	CCTNNNNNAGG	1 cut(s)	1427
BstF5I	GGATG	3 cut(s)	1138, 1513, 1792
BstFNI	CGCG	3 cut(s)	315, 991, 1894
BstHHI	GCGC	1 cut(s)	1548
BstMAI	GTCTC	3 cut(s)	401, 1192, 2402
BstNI	CCWGG	1 cut(s)	584
BstNSI	RCATGY	7 cut(s)	146, 1180, 1730, 1914, 2143, 2172, 2278
BstPI	GGTNACC	1 cut(s)	1133
BstSCI	CCNGG	4 cut(s)	582, 2011, 2208, 2577
BstSFI	CTRYAG	1 cut(s)	2215
BstSLI	GKGCMC	2 cut(s)	919, 1048
BstUI	CGCG	3 cut(s)	315, 991, 1894
BstV1I	GCAGC	3 cut(s)	1074, 1735, 2255
BstV2I	GAAGAC	1 cut(s)	2284
BstX2I	RGATCY	4 cut(s)	235, 420, 1636, 2560
BstYI	RGATCY	4 cut(s)	235, 420, 1636, 2560
BstZ17I	GTATAC	1 cut(s)	966
BsuRI	GGCC	6 cut(s)	78, 396, 470, 627, 2016, 2109
BtgI	CCRYGG	3 cut(s)	73, 471, 1349
BtsCI	GGATG	3 cut(s)	1138, 1513, 1792
BtsIMutI	CAGTG	3 cut(s)	190, 1422, 2418
BveI	ACCTGC	1 cut(s)	1549
Cac8I	GCNNGC	8 cut(s)	313, 1092, 1544, 1570, 2120, 2137, 2141, 2206
CaiI	CAGNNNCTG	1 cut(s)	1087
CciI	TCATGA	1 cut(s)	1426
CfoI	GCGC	1 cut(s)	1548
Cfr10I	RCCGGY	1 cut(s)	2135
Cfr13I	GGNCC	5 cut(s)	626, 929, 1420, 2015, 2108
Csp6I	GTAC	9 cut(s)	102, 659, 710, 993, 1079, 1210, 1562, 1864, 2416
CviQI	GTAC	9 cut(s)	102, 659, 710, 993, 1079, 1210, 1562, 1864, 2416
DdeI	CTNAG	8 cut(s)	450, 1170, 1380, 1457, 1692, 1825, 1838, 2362
DraI	TTTAAA	2 cut(s)	154, 688
DraIII	CACNNNGTG	1 cut(s)	1043
EaeI	YGGCCR	2 cut(s)	76, 394
Eam1104I	CTCTTC	1 cut(s)	2527
EarI	CTCTTC	1 cut(s)	2527
Eco130I	CCWWGG	8 cut(s)	73, 471, 646, 1349, 1593, 2191, 2302, 2394
Eco31I	GGTCTC	1 cut(s)	2402
Eco47I	GGWCC	2 cut(s)	929, 1420
Eco57I	CTGAAG	2 cut(s)	728, 920
Eco88I	CYCGRG	1 cut(s)	2328
Eco91I	GGTNACC	1 cut(s)	1133
EcoNI	CCTNNNNNAGG	1 cut(s)	1427
EcoO65I	GGTNACC	1 cut(s)	1133
EcoRII	CCWGG	1 cut(s)	582
EcoT14I	CCWWGG	8 cut(s)	73, 471, 646, 1349, 1593, 2191, 2302, 2394
EcoT22I	ATGCAT	2 cut(s)	1628, 1792
ErhI	CCWWGG	8 cut(s)	73, 471, 646, 1349, 1593, 2191, 2302, 2394
FaqI	GGGAC	2 cut(s)	1135, 1246
FauI	CCCGC	1 cut(s)	2539
FauNDI	CATATG	1 cut(s)	1538
FblI	GTMKAC	3 cut(s)	86, 965, 1858
Fnu4HI	GCNGC	4 cut(s)	1088, 1724, 2261, 2269
FokI	GGATG	3 cut(s)	1125, 1520, 1799
Fsp4HI	GCNGC	4 cut(s)	1088, 1724, 2261, 2269
FspBI	CTAG	6 cut(s)	411, 749, 827, 920, 2234, 2456
GlaI	GCGC	1 cut(s)	1547
GluI	GCNGC	4 cut(s)	1088, 1724, 2261, 2269
GsaI	CCCAGC	1 cut(s)	2126
GsuI	CTGGAG	2 cut(s)	234, 1431
HaeIII	GGCC	6 cut(s)	78, 396, 470, 627, 2016, 2109
HapII	CCGG	4 cut(s)	2013, 2136, 2210, 2578
HhaI	GCGC	1 cut(s)	1548
Hin6I	GCGC	1 cut(s)	1546
HinP1I	GCGC	1 cut(s)	1546
HincII	GTYRAC	1 cut(s)	1437
HindII	GTYRAC	1 cut(s)	1437
HinfI	GANTC	4 cut(s)	1292, 1823, 2196, 2351
HpaII	CCGG	4 cut(s)	2013, 2136, 2210, 2578
HphI	GGTGA	5 cut(s)	1082, 1145, 1336, 1434, 2303
Hpy188I	TCNGA	6 cut(s)	642, 900, 1107, 1587, 2489, 2588
Hpy99I	CGWCG	1 cut(s)	785
HpyCH4III	ACNGT	7 cut(s)	347, 889, 1237, 1417, 1757, 1863, 2216
HpyCH4IV	ACGT	3 cut(s)	760, 783, 1315
HpyF3I	CTNAG	8 cut(s)	450, 1170, 1380, 1457, 1692, 1825, 1838, 2362
HpySE526I	ACGT	3 cut(s)	760, 783, 1315
HspAI	GCGC	1 cut(s)	1546
KroI	GCCGGC	1 cut(s)	2135
KroNI	GCCGGC	1 cut(s)	2137
LmnI	GCTCC	5 cut(s)	253, 2009, 2132, 2254, 2451
Lsp1109I	GCAGC	3 cut(s)	1074, 1735, 2255
LweI	GCATC	5 cut(s)	508, 595, 1180, 1498, 1777
MaeI	CTAG	6 cut(s)	411, 749, 827, 920, 2234, 2456
MaeII	ACGT	3 cut(s)	760, 783, 1315
MaeIII	GTNAC	7 cut(s)	157, 323, 445, 761, 832, 1133, 1147
MbiI	CCGCTC	1 cut(s)	2335
MboII	GAAGA	8 cut(s)	426, 827, 887, 1008, 1367, 1922, 2284, 2544
MflI	RGATCY	4 cut(s)	235, 420, 1636, 2560
MhlI	GDGCHC	6 cut(s)	433, 919, 1004, 1048, 2251, 2311
MlsI	TGGCCA	2 cut(s)	78, 396
MluNI	TGGCCA	2 cut(s)	78, 396
MlyI	GAGTC	3 cut(s)	1817, 2205, 2345
MmeI	TCCRAC	5 cut(s)	33, 321, 526, 665, 1610
Mox20I	TGGCCA	2 cut(s)	78, 396
Mph1103I	ATGCAT	2 cut(s)	1628, 1792
MroNI	GCCGGC	1 cut(s)	2135
MscI	TGGCCA	2 cut(s)	78, 396
MseI	TTAA	7 cut(s)	20, 153, 299, 687, 882, 1530, 1997
MslI	CAYNNNNRTG	2 cut(s)	402, 1713
Msp20I	TGGCCA	2 cut(s)	78, 396
MspA1I	CMGCKG	1 cut(s)	664
MspI	CCGG	4 cut(s)	2013, 2136, 2210, 2578
MspR9I	CCNGG	4 cut(s)	584, 2013, 2210, 2579
Mva1269I	GAATGC	3 cut(s)	828, 2176, 2427
MvaI	CCWGG	1 cut(s)	584
MvnI	CGCG	3 cut(s)	315, 991, 1894
NaeI	GCCGGC	1 cut(s)	2137
NciI	CCSGG	3 cut(s)	2013, 2210, 2579
NcoI	CCATGG	3 cut(s)	73, 471, 1349
NdeI	CATATG	1 cut(s)	1538
NgoMIV	GCCGGC	1 cut(s)	2135
NlaIV	GGNNCC	3 cut(s)	1584, 2134, 2562
NmeAIII	GCCGAG	3 cut(s)	55, 63, 502
NmuCI	GTSAC	4 cut(s)	157, 832, 1133, 1147
NsiI	ATGCAT	2 cut(s)	1628, 1792
NspI	RCATGY	7 cut(s)	146, 1180, 1730, 1914, 2143, 2172, 2278
PaeI	GCATGC	1 cut(s)	2143
PaeR7I	CTCGAG	1 cut(s)	2328
PagI	TCATGA	1 cut(s)	1426
PciI	ACATGT	2 cut(s)	2168, 2274
PctI	GAATGC	3 cut(s)	828, 2176, 2427
PdiI	GCCGGC	1 cut(s)	2137
PfeI	GAWTC	1 cut(s)	1292
Pfl23II	CGTACG	1 cut(s)	709
PfoI	TCCNGGA	1 cut(s)	2577
PkrI	GCNGC	4 cut(s)	1089, 1725, 2262, 2270
PleI	GAGTC	3 cut(s)	1817, 2204, 2345
PpsI	GAGTC	3 cut(s)	1817, 2204, 2345
PscI	ACATGT	2 cut(s)	2168, 2274
PshBI	ATTAAT	1 cut(s)	1997
Psp6I	CCWGG	1 cut(s)	582
PspEI	GGTNACC	1 cut(s)	1133
PspFI	CCCAGC	1 cut(s)	2122
PspGI	CCWGG	1 cut(s)	582
PspLI	CGTACG	1 cut(s)	709
PspN4I	GGNNCC	3 cut(s)	1584, 2134, 2562
PspPI	GGNCC	5 cut(s)	626, 929, 1420, 2015, 2108
PstNI	CAGNNNCTG	1 cut(s)	1087
PsuI	RGATCY	4 cut(s)	235, 420, 1636, 2560
RsaI	GTAC	9 cut(s)	103, 660, 711, 994, 1080, 1211, 1563, 1865, 2417
RsaNI	GTAC	9 cut(s)	102, 659, 710, 993, 1079, 1210, 1562, 1864, 2416
RseI	CAYNNNNRTG	2 cut(s)	402, 1713
SaqAI	TTAA	7 cut(s)	20, 153, 299, 687, 882, 1530, 1997
SatI	GCNGC	4 cut(s)	1088, 1724, 2261, 2269
Sau96I	GGNCC	5 cut(s)	626, 929, 1420, 2015, 2108
SchI	GAGTC	3 cut(s)	1817, 2205, 2345
ScrFI	CCNGG	4 cut(s)	584, 2013, 2210, 2579
SduI	GDGCHC	6 cut(s)	433, 919, 1004, 1048, 2251, 2311
SfaNI	GCATC	5 cut(s)	508, 595, 1180, 1498, 1777
SfcI	CTRYAG	1 cut(s)	2215
Sfr274I	CTCGAG	1 cut(s)	2328
SinI	GGWCC	2 cut(s)	929, 1420
SlaI	CTCGAG	1 cut(s)	2328
SmiMI	CAYNNNNRTG	2 cut(s)	402, 1713
SmlI	CTYRAG	4 cut(s)	257, 910, 969, 2328
SmoI	CTYRAG	4 cut(s)	257, 910, 969, 2328
SpeI	ACTAGT	1 cut(s)	2455
SphI	GCATGC	1 cut(s)	2143
SsiI	CCGC	7 cut(s)	315, 664, 989, 1894, 2260, 2335, 2546
SspMI	CTAG	6 cut(s)	411, 749, 827, 920, 2234, 2456
StyD4I	CCNGG	4 cut(s)	582, 2011, 2208, 2577
StyI	CCWWGG	8 cut(s)	73, 471, 646, 1349, 1593, 2191, 2302, 2394
TaaI	ACNGT	7 cut(s)	347, 889, 1237, 1417, 1757, 1863, 2216
TaiI	ACGT	3 cut(s)	763, 786, 1318
TaqI	TCGA	4 cut(s)	384, 780, 2079, 2329
TatI	WGTACW	3 cut(s)	1078, 1209, 2415
TauI	GCSGC	1 cut(s)	2263
TfiI	GAWTC	1 cut(s)	1292
Tru1I	TTAA	7 cut(s)	20, 153, 299, 687, 882, 1530, 1997
Tru9I	TTAA	7 cut(s)	20, 153, 299, 687, 882, 1530, 1997
TscAI	CASTG	3 cut(s)	190, 1422, 2425
TseFI	GTSAC	4 cut(s)	157, 832, 1133, 1147
TseI	GCWGC	3 cut(s)	1087, 1723, 2268
Tsp45I	GTSAC	4 cut(s)	157, 832, 1133, 1147
TspGWI	ACGGA	6 cut(s)	52, 119, 609, 1143, 1520, 2418
TspRI	CASTG	3 cut(s)	190, 1422, 2425
VneI	GTGCAC	2 cut(s)	915, 1044
VpaK11BI	GGWCC	2 cut(s)	929, 1420
VspI	ATTAAT	1 cut(s)	1997
XagI	CCTNNNNNAGG	1 cut(s)	1427
XapI	RAATTY	2 cut(s)	116, 2316
XbaI	TCTAGA	1 cut(s)	2233
XceI	RCATGY	7 cut(s)	146, 1180, 1730, 1914, 2143, 2172, 2278
XcmI	CCANNNNNNNNNTGG	1 cut(s)	699
XhoI	CTCGAG	1 cut(s)	2328
XmiI	GTMKAC	3 cut(s)	86, 965, 1858
XspI	CTAG	6 cut(s)	411, 749, 827, 920, 2234, 2456
Zsp2I	ATGCAT	2 cut(s)	1628, 1792

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.