Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G32000 AT2G32000 |
| fragaria_vesca | FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 |
| malus_domestica | MD04G1023600.v1.1 |
| prunus_persica | Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 |
| pyrus_communis | pycom04g02020 |
| rosa_chinensis | RchiOBHm_Chr4g0412031 |
| rosa_laevigata | RLG00000008327 |
| rosa_multiflora | Rmu_co8041726.1_g000001 Rmu_sc0000711.1_g000051 |
| rosa_roxburghii | Rroxscaffold_1G00042480 Rroxscaffold_5G00346360 Rroxscaffold_5G00356250 Rroxscaffold_5G00356340 |
| rosa_rugosa | Rorug04G0106700 Rorug04G0106800 Rorug04G0106900 |
| rosa_samantha | Rh4AG171100 Rh4BG168800 Rh4BG169700 Rh4CG181900 Rh4DG164200 Rh4DG165300 |
| rosa_wichuraiana | Rw0G007770 Rw0G008800 Rw4G014260 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1040 |
| AatII | GACGTC | 1 cut(s) | 699 |
| Acc36I | ACCTGC | 3 cut(s) | 1040, 1468, 1681 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1037 |
| AccI | GTMKAC | 2 cut(s) | 5, 884 |
| AccII | CGCG | 1 cut(s) | 910 |
| AciI | CCGC | 2 cut(s) | 908, 1576 |
| AclWI | GGATC | 3 cut(s) | 140, 162, 1544 |
| AcoI | YGGCCR | 1 cut(s) | 313 |
| AcsI | RAATTY | 3 cut(s) | 35, 617, 1193 |
| AcuI | CTGAAG | 1 cut(s) | 647 |
| AcyI | GRCGYC | 1 cut(s) | 696 |
| AdeI | CACNNNGTG | 1 cut(s) | 962 |
| AfaI | GTAC | 7 cut(s) | 30, 66, 579, 630, 913, 999, 1482 |
| AfeI | AGCGCT | 1 cut(s) | 584 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 566, 856, 985, 986, 1037, 1348 |
| AflIII | ACRYGT | 1 cut(s) | 1234 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1185, 1217 |
| AluBI | AGCT | 6 cut(s) | 167, 230, 667, 903, 1390, 1487 |
| AluI | AGCT | 6 cut(s) | 167, 230, 667, 903, 1390, 1487 |
| Alw21I | GWGCWC | 4 cut(s) | 352, 838, 923, 967 |
| Alw44I | GTGCAC | 3 cut(s) | 348, 834, 963 |
| AlwI | GGATC | 3 cut(s) | 140, 162, 1544 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 143, 1006 |
| Ama87I | CYCGRG | 1 cut(s) | 231 |
| Aor51HI | AGCGCT | 1 cut(s) | 584 |
| AoxI | GGCC | 4 cut(s) | 313, 387, 545, 847 |
| ApaLI | GTGCAC | 3 cut(s) | 348, 834, 963 |
| ApeKI | GCWGC | 3 cut(s) | 1006, 1591, 1642 |
| ApoI | RAATTY | 3 cut(s) | 35, 617, 1193 |
| AspLEI | GCGC | 2 cut(s) | 585, 1467 |
| AspS9I | GGNCC | 3 cut(s) | 545, 848, 1339 |
| AsuC2I | CCSGG | 1 cut(s) | 980 |
| AsuHPI | GGTGA | 3 cut(s) | 1064, 1255, 1353 |
| AsuNHI | GCTAGC | 1 cut(s) | 17 |
| AvaI | CYCGRG | 1 cut(s) | 231 |
| AvaII | GGWCC | 1 cut(s) | 1339 |
| AxyI | CCTNAGG | 1 cut(s) | 1513 |
| BaeGI | GKGCMC | 3 cut(s) | 352, 838, 967 |
| BalI | TGGCCA | 1 cut(s) | 315 |
| Bbv12I | GWGCWC | 4 cut(s) | 352, 838, 923, 967 |
| BbvI | GCAGC | 3 cut(s) | 993, 1603, 1654 |
| BccI | CCATC | 1 cut(s) | 731 |
| BclI | TGATCA | 1 cut(s) | 1054 |
| BcnI | CCSGG | 1 cut(s) | 980 |
| BfaI | CTAG | 4 cut(s) | 18, 107, 668, 839 |
| BfmI | CTRYAG | 1 cut(s) | 432 |
| BfoI | RGCGCY | 1 cut(s) | 586 |
| BfuAI | ACCTGC | 3 cut(s) | 1040, 1468, 1681 |
| BglII | AGATCT | 1 cut(s) | 1555 |
| BisI | GCNGC | 3 cut(s) | 1007, 1592, 1643 |
| BlsI | GCNGC | 3 cut(s) | 1008, 1593, 1644 |
| Bme1390I | CCNGG | 1 cut(s) | 980 |
| Bme18I | GGWCC | 1 cut(s) | 1339 |
| BmeT110I | CYCGRG | 1 cut(s) | 231 |
| BmgT120I | GGNCC | 3 cut(s) | 545, 848, 1339 |
| BmiI | GGNNCC | 1 cut(s) | 1503 |
| BmrFI | CCNGG | 1 cut(s) | 980 |
| BmrI | ACTGGG | 1 cut(s) | 682 |
| BmsI | GCATC | 7 cut(s) | 427, 514, 1036, 1094, 1099, 1417, 1439 |
| BmtI | GCTAGC | 1 cut(s) | 21 |
| BmuI | ACTGGG | 1 cut(s) | 682 |
| BpmI | CTGGAG | 2 cut(s) | 153, 1350 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1611 |
| BpuEI | CTTGAG | 1 cut(s) | 873 |
| BpuMI | CCSGG | 1 cut(s) | 980 |
| BsaBI | GATNNNNATC | 2 cut(s) | 267, 1554 |
| BsaHI | GRCGYC | 1 cut(s) | 696 |
| BsaJI | CCNNGG | 3 cut(s) | 390, 565, 1268 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1144, 1174, 1348, 1378 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 566, 856, 985, 986, 1037, 1348 |
| Bse1I | ACTGG | 3 cut(s) | 137, 170, 677 |
| Bse21I | CCTNAGG | 1 cut(s) | 1513 |
| Bse8I | GATNNNNATC | 2 cut(s) | 267, 1554 |
| BseDI | CCNNGG | 3 cut(s) | 390, 565, 1268 |
| BseGI | GGATG | 2 cut(s) | 1057, 1432 |
| BseJI | GATNNNNATC | 2 cut(s) | 267, 1554 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 566, 856, 985, 986, 1037, 1348 |
| BseMII | CTCAG | 1 cut(s) | 1080 |
| BseNI | ACTGG | 3 cut(s) | 137, 170, 677 |
| BseSI | GKGCMC | 3 cut(s) | 352, 838, 967 |
| BseXI | GCAGC | 3 cut(s) | 993, 1603, 1654 |
| BseYI | CCCAGC | 1 cut(s) | 1099 |
| BsgI | GTGCAG | 2 cut(s) | 1496, 1661 |
| Bsh1236I | CGCG | 1 cut(s) | 910 |
| BshFI | GGCC | 4 cut(s) | 315, 389, 547, 849 |
| BsiHKAI | GWGCWC | 4 cut(s) | 352, 838, 923, 967 |
| BsiHKCI | CYCGRG | 1 cut(s) | 231 |
| BsiSI | CCGG | 1 cut(s) | 980 |
| BslFI | GGGAC | 2 cut(s) | 1054, 1165 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 566, 856, 985, 986, 1037, 1348 |
| BsmFI | GGGAC | 2 cut(s) | 1054, 1165 |
| BsnI | GGCC | 4 cut(s) | 315, 389, 547, 849 |
| BsoBI | CYCGRG | 1 cut(s) | 231 |
| Bsp1286I | GDGCHC | 4 cut(s) | 352, 838, 923, 967 |
| Bsp1407I | TGTACA | 3 cut(s) | 64, 577, 997 |
| Bsp143I | GATC | 8 cut(s) | 145, 154, 268, 1034, 1054, 1549, 1555, 1624 |
| Bsp19I | CCATGG | 2 cut(s) | 390, 1268 |
| BspACI | CCGC | 2 cut(s) | 908, 1576 |
| BspANI | GGCC | 4 cut(s) | 315, 389, 547, 849 |
| BspCNI | CTCAG | 1 cut(s) | 1081 |
| BspFNI | CGCG | 1 cut(s) | 910 |
| BspHI | TCATGA | 1 cut(s) | 1345 |
| BspLI | GGNNCC | 1 cut(s) | 1503 |
| BspMI | ACCTGC | 3 cut(s) | 1040, 1468, 1681 |
| BspOI | GCTAGC | 1 cut(s) | 21 |
| BspPI | GGATC | 3 cut(s) | 140, 162, 1544 |
| BsrGI | TGTACA | 3 cut(s) | 64, 577, 997 |
| BsrI | ACTGG | 3 cut(s) | 137, 170, 677 |
| BssECI | CCNNGG | 3 cut(s) | 390, 565, 1268 |
| BssMI | GATC | 8 cut(s) | 145, 154, 268, 1034, 1054, 1549, 1555, 1624 |
| BssNAI | GTATAC | 1 cut(s) | 885 |
| BssNI | GRCGYC | 1 cut(s) | 696 |
| BssT1I | CCWWGG | 3 cut(s) | 390, 565, 1268 |
| Bst1107I | GTATAC | 1 cut(s) | 885 |
| Bst4CI | ACNGT | 7 cut(s) | 69, 139, 808, 1019, 1156, 1659, 1676 |
| BstACI | GRCGYC | 1 cut(s) | 696 |
| BstAUI | TGTACA | 3 cut(s) | 64, 577, 997 |
| BstC8I | GCNNGC | 3 cut(s) | 19, 212, 1489 |
| BstDEI | CTNAG | 4 cut(s) | 1089, 1376, 1513, 1611 |
| BstDSI | CCRYGG | 2 cut(s) | 390, 1268 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1346 |
| BstF5I | GGATG | 2 cut(s) | 1057, 1432 |
| BstFNI | CGCG | 1 cut(s) | 910 |
| BstH2I | RGCGCY | 1 cut(s) | 586 |
| BstHHI | GCGC | 2 cut(s) | 585, 1467 |
| BstKTI | GATC | 8 cut(s) | 148, 157, 271, 1037, 1057, 1552, 1558, 1627 |
| BstMBI | GATC | 8 cut(s) | 145, 154, 268, 1034, 1054, 1549, 1555, 1624 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 437, 589, 833, 900, 1396 |
| BstNSI | RCATGY | 2 cut(s) | 98, 1649 |
| BstSCI | CCNGG | 1 cut(s) | 978 |
| BstSFI | CTRYAG | 1 cut(s) | 432 |
| BstSLI | GKGCMC | 3 cut(s) | 352, 838, 967 |
| BstUI | CGCG | 1 cut(s) | 910 |
| BstV1I | GCAGC | 3 cut(s) | 993, 1603, 1654 |
| BstX2I | RGATCY | 2 cut(s) | 154, 1555 |
| BstYI | RGATCY | 2 cut(s) | 154, 1555 |
| BstZ17I | GTATAC | 1 cut(s) | 885 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1513 |
| BsuRI | GGCC | 4 cut(s) | 315, 389, 547, 849 |
| BtgI | CCRYGG | 2 cut(s) | 390, 1268 |
| BtsCI | GGATG | 2 cut(s) | 1057, 1432 |
| BtsIMutI | CAGTG | 3 cut(s) | 141, 813, 1011 |
| BveI | ACCTGC | 3 cut(s) | 1040, 1468, 1681 |
| Cac8I | GCNNGC | 3 cut(s) | 19, 212, 1489 |
| CaiI | CAGNNNCTG | 2 cut(s) | 143, 1006 |
| CciI | TCATGA | 1 cut(s) | 1345 |
| CfoI | GCGC | 2 cut(s) | 585, 1467 |
| Cfr13I | GGNCC | 3 cut(s) | 545, 848, 1339 |
| Csp6I | GTAC | 7 cut(s) | 29, 65, 578, 629, 912, 998, 1481 |
| CviAII | CATG | 8 cut(s) | 32, 62, 95, 391, 549, 1269, 1346, 1646 |
| CviQI | GTAC | 7 cut(s) | 29, 65, 578, 629, 912, 998, 1481 |
| DdeI | CTNAG | 4 cut(s) | 1089, 1376, 1513, 1611 |
| DpnI | GATC | 8 cut(s) | 147, 156, 270, 1036, 1056, 1551, 1557, 1626 |
| DpnII | GATC | 8 cut(s) | 145, 154, 268, 1034, 1054, 1549, 1555, 1624 |
| DraI | TTTAAA | 2 cut(s) | 73, 607 |
| DraIII | CACNNNGTG | 1 cut(s) | 962 |
| EaeI | YGGCCR | 1 cut(s) | 313 |
| Eco130I | CCWWGG | 3 cut(s) | 390, 565, 1268 |
| Eco32I | GATATC | 1 cut(s) | 1573 |
| Eco47I | GGWCC | 1 cut(s) | 1339 |
| Eco47III | AGCGCT | 1 cut(s) | 584 |
| Eco57I | CTGAAG | 1 cut(s) | 647 |
| Eco81I | CCTNAGG | 1 cut(s) | 1513 |
| Eco88I | CYCGRG | 1 cut(s) | 231 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1346 |
| EcoRV | GATATC | 1 cut(s) | 1573 |
| EcoT14I | CCWWGG | 3 cut(s) | 390, 565, 1268 |
| EcoT22I | ATGCAT | 1 cut(s) | 1432 |
| ErhI | CCWWGG | 3 cut(s) | 390, 565, 1268 |
| FaeI | CATG | 8 cut(s) | 35, 65, 98, 394, 552, 1272, 1349, 1649 |
| FaqI | GGGAC | 2 cut(s) | 1054, 1165 |
| FatI | CATG | 8 cut(s) | 31, 61, 94, 390, 548, 1268, 1345, 1645 |
| FauNDI | CATATG | 1 cut(s) | 1457 |
| FbaI | TGATCA | 1 cut(s) | 1054 |
| FblI | GTMKAC | 2 cut(s) | 5, 884 |
| Fnu4HI | GCNGC | 3 cut(s) | 1007, 1592, 1643 |
| FokI | GGATG | 2 cut(s) | 1044, 1439 |
| Fsp4HI | GCNGC | 3 cut(s) | 1007, 1592, 1643 |
| FspBI | CTAG | 4 cut(s) | 18, 107, 668, 839 |
| GlaI | GCGC | 2 cut(s) | 584, 1466 |
| GluI | GCNGC | 3 cut(s) | 1007, 1592, 1643 |
| GsaI | CCCAGC | 1 cut(s) | 1103 |
| GsuI | CTGGAG | 2 cut(s) | 153, 1350 |
| HaeII | RGCGCY | 1 cut(s) | 586 |
| HaeIII | GGCC | 4 cut(s) | 315, 389, 547, 849 |
| HapII | CCGG | 1 cut(s) | 980 |
| HhaI | GCGC | 2 cut(s) | 585, 1467 |
| Hin1I | GRCGYC | 1 cut(s) | 696 |
| Hin1II | CATG | 8 cut(s) | 35, 65, 98, 394, 552, 1272, 1349, 1649 |
| Hin6I | GCGC | 2 cut(s) | 583, 1465 |
| HinP1I | GCGC | 2 cut(s) | 583, 1465 |
| HinfI | GANTC | 3 cut(s) | 57, 811, 1416 |
| HpaII | CCGG | 1 cut(s) | 980 |
| HphI | GGTGA | 3 cut(s) | 1064, 1255, 1353 |
| Hpy166II | GTNNAC | 8 cut(s) | 6, 350, 578, 836, 885, 965, 1139, 1339 |
| Hpy188I | TCNGA | 4 cut(s) | 467, 766, 1090, 1664 |
| Hpy188III | TCNNGA | 9 cut(s) | 107, 116, 152, 272, 521, 1163, 1346, 1367, 1553 |
| Hpy8I | GTNNAC | 8 cut(s) | 6, 350, 578, 836, 885, 965, 1139, 1339 |
| Hpy99I | CGWCG | 1 cut(s) | 701 |
| HpyAV | CCTTC | 6 cut(s) | 269, 379, 801, 942, 1240, 1352 |
| HpyCH4III | ACNGT | 7 cut(s) | 69, 139, 808, 1019, 1156, 1659, 1676 |
| HpyCH4IV | ACGT | 3 cut(s) | 27, 696, 1234 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 437, 589, 833, 900, 1396 |
| HpyF3I | CTNAG | 4 cut(s) | 1089, 1376, 1513, 1611 |
| HpySE526I | ACGT | 3 cut(s) | 27, 696, 1234 |
| Hsp92I | GRCGYC | 1 cut(s) | 696 |
| Hsp92II | CATG | 8 cut(s) | 35, 65, 98, 394, 552, 1272, 1349, 1649 |
| HspAI | GCGC | 2 cut(s) | 583, 1465 |
| Ksp22I | TGATCA | 1 cut(s) | 1054 |
| Kzo9I | GATC | 8 cut(s) | 145, 154, 268, 1034, 1054, 1549, 1555, 1624 |
| LmnI | GCTCC | 2 cut(s) | 172, 676 |
| Lsp1109I | GCAGC | 3 cut(s) | 993, 1603, 1654 |
| LweI | GCATC | 7 cut(s) | 427, 514, 1036, 1094, 1099, 1417, 1439 |
| MaeI | CTAG | 4 cut(s) | 18, 107, 668, 839 |
| MaeII | ACGT | 3 cut(s) | 27, 696, 1234 |
| MaeIII | GTNAC | 7 cut(s) | 76, 139, 242, 364, 680, 751, 1066 |
| MalI | GATC | 8 cut(s) | 147, 156, 270, 1036, 1056, 1551, 1557, 1626 |
| MboI | GATC | 8 cut(s) | 145, 154, 268, 1034, 1054, 1549, 1555, 1624 |
| MboII | GAAGA | 7 cut(s) | 351, 746, 806, 927, 1207, 1286, 1420 |
| MfeI | CAATTG | 1 cut(s) | 1200 |
| MflI | RGATCY | 2 cut(s) | 154, 1555 |
| MhlI | GDGCHC | 4 cut(s) | 352, 838, 923, 967 |
| MlsI | TGGCCA | 1 cut(s) | 315 |
| MluCI | AATT | 9 cut(s) | 35, 617, 686, 741, 1193, 1200, 1301, 1392, 1468 |
| MluNI | TGGCCA | 1 cut(s) | 315 |
| MmeI | TCCRAC | 3 cut(s) | 445, 1529, 1687 |
| Mox20I | TGGCCA | 1 cut(s) | 315 |
| Mph1103I | ATGCAT | 1 cut(s) | 1432 |
| MscI | TGGCCA | 1 cut(s) | 315 |
| MseI | TTAA | 5 cut(s) | 72, 218, 606, 801, 1449 |
| MslI | CAYNNNNRTG | 2 cut(s) | 321, 1632 |
| Msp20I | TGGCCA | 1 cut(s) | 315 |
| MspI | CCGG | 1 cut(s) | 980 |
| MspR9I | CCNGG | 1 cut(s) | 980 |
| MssI | GTTTAAAC | 1 cut(s) | 607 |
| MunI | CAATTG | 1 cut(s) | 1200 |
| MvnI | CGCG | 1 cut(s) | 910 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 437, 589, 833, 900, 1396 |
| NciI | CCSGG | 1 cut(s) | 980 |
| NcoI | CCATGG | 2 cut(s) | 390, 1268 |
| NdeI | CATATG | 1 cut(s) | 1457 |
| NdeII | GATC | 8 cut(s) | 145, 154, 268, 1034, 1054, 1549, 1555, 1624 |
| NheI | GCTAGC | 1 cut(s) | 17 |
| NlaIII | CATG | 8 cut(s) | 35, 65, 98, 394, 552, 1272, 1349, 1649 |
| NlaIV | GGNNCC | 1 cut(s) | 1503 |
| NmuCI | GTSAC | 3 cut(s) | 76, 139, 751 |
| NsiI | ATGCAT | 1 cut(s) | 1432 |
| NspI | RCATGY | 2 cut(s) | 98, 1649 |
| PagI | TCATGA | 1 cut(s) | 1345 |
| PaqCI | CACCTGC | 1 cut(s) | 1040 |
| PfeI | GAWTC | 3 cut(s) | 57, 811, 1416 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1037 |
| PkrI | GCNGC | 3 cut(s) | 1008, 1593, 1644 |
| PmeI | GTTTAAAC | 1 cut(s) | 607 |
| PspFI | CCCAGC | 1 cut(s) | 1099 |
| PspN4I | GGNNCC | 1 cut(s) | 1503 |
| PspPI | GGNCC | 3 cut(s) | 545, 848, 1339 |
| PstNI | CAGNNNCTG | 2 cut(s) | 143, 1006 |
| PsuI | RGATCY | 2 cut(s) | 154, 1555 |
| RsaI | GTAC | 7 cut(s) | 30, 66, 579, 630, 913, 999, 1482 |
| RsaNI | GTAC | 7 cut(s) | 29, 65, 578, 629, 912, 998, 1481 |
| RseI | CAYNNNNRTG | 2 cut(s) | 321, 1632 |
| SaqAI | TTAA | 5 cut(s) | 72, 218, 606, 801, 1449 |
| SatI | GCNGC | 3 cut(s) | 1007, 1592, 1643 |
| Sau3AI | GATC | 8 cut(s) | 145, 154, 268, 1034, 1054, 1549, 1555, 1624 |
| Sau96I | GGNCC | 3 cut(s) | 545, 848, 1339 |
| ScrFI | CCNGG | 1 cut(s) | 980 |
| SduI | GDGCHC | 4 cut(s) | 352, 838, 923, 967 |
| SfaNI | GCATC | 7 cut(s) | 427, 514, 1036, 1094, 1099, 1417, 1439 |
| SfcI | CTRYAG | 1 cut(s) | 432 |
| SinI | GGWCC | 1 cut(s) | 1339 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 321, 1632 |
| SmlI | CTYRAG | 1 cut(s) | 888 |
| SmoI | CTYRAG | 1 cut(s) | 888 |
| Sse9I | AATT | 9 cut(s) | 35, 617, 686, 741, 1193, 1200, 1301, 1392, 1468 |
| SsiI | CCGC | 2 cut(s) | 908, 1576 |
| SspMI | CTAG | 4 cut(s) | 18, 107, 668, 839 |
| StyD4I | CCNGG | 1 cut(s) | 978 |
| StyI | CCWWGG | 3 cut(s) | 390, 565, 1268 |
| TaaI | ACNGT | 7 cut(s) | 69, 139, 808, 1019, 1156, 1659, 1676 |
| TaiI | ACGT | 3 cut(s) | 30, 699, 1237 |
| TaqI | TCGA | 3 cut(s) | 442, 522, 699 |
| TasI | AATT | 9 cut(s) | 35, 617, 686, 741, 1193, 1200, 1301, 1392, 1468 |
| TatI | WGTACW | 3 cut(s) | 64, 577, 997 |
| TfiI | GAWTC | 3 cut(s) | 57, 811, 1416 |
| Tru1I | TTAA | 5 cut(s) | 72, 218, 606, 801, 1449 |
| Tru9I | TTAA | 5 cut(s) | 72, 218, 606, 801, 1449 |
| TscAI | CASTG | 3 cut(s) | 148, 813, 1018 |
| TseFI | GTSAC | 3 cut(s) | 76, 139, 751 |
| TseI | GCWGC | 3 cut(s) | 1006, 1591, 1642 |
| Tsp45I | GTSAC | 3 cut(s) | 76, 139, 751 |
| TspDTI | ATGAA | 7 cut(s) | 48, 339, 672, 886, 1135, 1334, 1433 |
| TspGWI | ACGGA | 2 cut(s) | 38, 1439 |
| TspRI | CASTG | 3 cut(s) | 148, 813, 1018 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1037 |
| VneI | GTGCAC | 3 cut(s) | 348, 834, 963 |
| VpaK11BI | GGWCC | 1 cut(s) | 1339 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1346 |
| XapI | RAATTY | 3 cut(s) | 35, 617, 1193 |
| XbaI | TCTAGA | 1 cut(s) | 106 |
| XceI | RCATGY | 2 cut(s) | 98, 1649 |
| XmiI | GTMKAC | 2 cut(s) | 5, 884 |
| XspI | CTAG | 4 cut(s) | 18, 107, 668, 839 |
| ZraI | GACGTC | 1 cut(s) | 697 |
| Zsp2I | ATGCAT | 1 cut(s) | 1432 |