Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G32000 AT2G32000 |
| fragaria_vesca | FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 |
| malus_domestica | MD04G1023600.v1.1 |
| prunus_persica | Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 |
| pyrus_communis | pycom04g02020 |
| rosa_chinensis | RchiOBHm_Chr4g0412031 |
| rosa_laevigata | RLG00000008327 |
| rosa_multiflora | Rmu_co8041726.1_g000001 Rmu_sc0000711.1_g000051 |
| rosa_roxburghii | Rroxscaffold_1G00042480 Rroxscaffold_5G00346360 Rroxscaffold_5G00356250 Rroxscaffold_5G00356340 |
| rosa_rugosa | Rorug04G0106700 Rorug04G0106800 Rorug04G0106900 |
| rosa_samantha | Rh4AG171100 Rh4BG168800 Rh4BG169700 Rh4CG181900 Rh4DG164200 Rh4DG165300 |
| rosa_wichuraiana | Rw0G007770 Rw0G008800 Rw4G014260 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1103 |
| AatII | GACGTC | 1 cut(s) | 762 |
| Acc36I | ACCTGC | 2 cut(s) | 1103, 1531 |
| Acc65I | GGTACC | 1 cut(s) | 1925 |
| AccB1I | GGYRCC | 1 cut(s) | 1925 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1100 |
| AccBSI | CCGCTC | 1 cut(s) | 2317 |
| AccI | GTMKAC | 4 cut(s) | 68, 947, 1840, 2155 |
| AccII | CGCG | 1 cut(s) | 973 |
| AciI | CCGC | 4 cut(s) | 971, 1639, 2317, 2521 |
| AclWI | GGATC | 6 cut(s) | 203, 225, 1607, 2503, 2537, 2550 |
| AcoI | YGGCCR | 1 cut(s) | 376 |
| AcsI | RAATTY | 3 cut(s) | 98, 680, 1256 |
| AcuI | CTGAAG | 1 cut(s) | 710 |
| AcyI | GRCGYC | 1 cut(s) | 759 |
| AdeI | CACNNNGTG | 1 cut(s) | 1025 |
| AfaI | GTAC | 9 cut(s) | 93, 642, 693, 976, 1062, 1545, 1927, 2453, 2527 |
| AfeI | AGCGCT | 1 cut(s) | 647 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 629, 919, 1048, 1049, 1100, 1411, 1922, 2054, 2287 |
| AflIII | ACRYGT | 3 cut(s) | 1297, 2150, 2256 |
| AhlI | ACTAGT | 1 cut(s) | 2437 |
| AjnI | CCWGG | 1 cut(s) | 1993 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1248, 1280 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2196, 2228 |
| Alw21I | GWGCWC | 6 cut(s) | 415, 901, 986, 1030, 2233, 2277 |
| Alw26I | GTCTC | 1 cut(s) | 1883 |
| Alw44I | GTGCAC | 3 cut(s) | 411, 897, 1026 |
| AlwI | GGATC | 6 cut(s) | 203, 225, 1607, 2503, 2537, 2550 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 206, 1069 |
| Ama87I | CYCGRG | 1 cut(s) | 294 |
| Aor51HI | AGCGCT | 1 cut(s) | 647 |
| AoxI | GGCC | 5 cut(s) | 376, 450, 608, 910, 1996 |
| ApaLI | GTGCAC | 3 cut(s) | 411, 897, 1026 |
| ApeKI | GCWGC | 4 cut(s) | 1069, 1654, 1705, 2078 |
| ApoI | RAATTY | 3 cut(s) | 98, 680, 1256 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2054, 2086 |
| AseI | ATTAAT | 1 cut(s) | 1979 |
| Asp718I | GGTACC | 1 cut(s) | 1925 |
| AspLEI | GCGC | 2 cut(s) | 648, 1530 |
| AspS9I | GGNCC | 4 cut(s) | 608, 911, 1402, 1997 |
| AsuC2I | CCSGG | 1 cut(s) | 1043 |
| AsuHPI | GGTGA | 4 cut(s) | 1127, 1318, 1388, 1416 |
| AsuNHI | GCTAGC | 1 cut(s) | 80 |
| AvaI | CYCGRG | 1 cut(s) | 294 |
| AvaII | GGWCC | 1 cut(s) | 1402 |
| AxyI | CCTNAGG | 1 cut(s) | 1576 |
| BaeGI | GKGCMC | 4 cut(s) | 415, 901, 1030, 1958 |
| BalI | TGGCCA | 1 cut(s) | 378 |
| BamHI | GGATCC | 1 cut(s) | 2542 |
| BanI | GGYRCC | 1 cut(s) | 1925 |
| BanII | GRGCYC | 1 cut(s) | 2293 |
| Bbv12I | GWGCWC | 6 cut(s) | 415, 901, 986, 1030, 2233, 2277 |
| BbvI | GCAGC | 4 cut(s) | 1056, 1666, 1717, 2065 |
| BccI | CCATC | 3 cut(s) | 794, 1997, 2378 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2232, 2266 |
| BciT130I | CCWGG | 1 cut(s) | 1995 |
| BclI | TGATCA | 1 cut(s) | 1117 |
| BcnI | CCSGG | 1 cut(s) | 1043 |
| BcoDI | GTCTC | 1 cut(s) | 1883 |
| BcuI | ACTAGT | 1 cut(s) | 2437 |
| BfaI | CTAG | 6 cut(s) | 81, 170, 731, 902, 2216, 2438 |
| BfmI | CTRYAG | 2 cut(s) | 59, 495 |
| BfoI | RGCGCY | 1 cut(s) | 649 |
| BfuAI | ACCTGC | 2 cut(s) | 1103, 1531 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2004 |
| BglII | AGATCT | 1 cut(s) | 1618 |
| BisI | GCNGC | 4 cut(s) | 1070, 1655, 1706, 2079 |
| BlsI | GCNGC | 4 cut(s) | 1071, 1656, 1707, 2080 |
| Bme1390I | CCNGG | 2 cut(s) | 1043, 1995 |
| Bme18I | GGWCC | 1 cut(s) | 1402 |
| BmeT110I | CYCGRG | 1 cut(s) | 294 |
| BmgT120I | GGNCC | 4 cut(s) | 608, 911, 1402, 1997 |
| BmiI | GGNNCC | 3 cut(s) | 1566, 1927, 2544 |
| BmrFI | CCNGG | 2 cut(s) | 1043, 1995 |
| BmrI | ACTGGG | 2 cut(s) | 745, 2538 |
| BmsI | GCATC | 7 cut(s) | 490, 577, 1099, 1162, 1480, 1502, 1759 |
| BmtI | GCTAGC | 1 cut(s) | 84 |
| BmuI | ACTGGG | 2 cut(s) | 745, 2538 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2318, 2350 |
| BpmI | CTGGAG | 3 cut(s) | 216, 1413, 2295 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1674 |
| BpuEI | CTTGAG | 1 cut(s) | 936 |
| BpuMI | CCSGG | 1 cut(s) | 1043 |
| BsaAI | YACGTR | 1 cut(s) | 1953 |
| BsaBI | GATNNNNATC | 2 cut(s) | 330, 1617 |
| BsaHI | GRCGYC | 1 cut(s) | 759 |
| BsaJI | CCNNGG | 6 cut(s) | 453, 628, 1331, 2173, 2284, 2376 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 1207, 1237, 1411, 1441, 2435, 2465 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 629, 919, 1048, 1049, 1100, 1411, 1922, 2054, 2287 |
| Bse1I | ACTGG | 5 cut(s) | 200, 233, 740, 2407, 2533 |
| Bse21I | CCTNAGG | 1 cut(s) | 1576 |
| Bse8I | GATNNNNATC | 2 cut(s) | 330, 1617 |
| BseBI | CCWGG | 1 cut(s) | 1995 |
| BseDI | CCNNGG | 6 cut(s) | 453, 628, 1331, 2173, 2284, 2376 |
| BseGI | GGATG | 4 cut(s) | 1120, 1495, 1774, 2143 |
| BseJI | GATNNNNATC | 2 cut(s) | 330, 1617 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 629, 919, 1048, 1049, 1100, 1411, 1922, 2054, 2287 |
| BseMII | CTCAG | 5 cut(s) | 1143, 1821, 1834, 1931, 2316 |
| BseNI | ACTGG | 5 cut(s) | 200, 233, 740, 2407, 2533 |
| BseRI | GAGGAG | 1 cut(s) | 2267 |
| BseSI | GKGCMC | 4 cut(s) | 415, 901, 1030, 1958 |
| BseXI | GCAGC | 4 cut(s) | 1056, 1666, 1717, 2065 |
| BseYI | CCCAGC | 2 cut(s) | 1162, 2104 |
| BsgI | GTGCAG | 2 cut(s) | 1559, 1724 |
| Bsh1236I | CGCG | 1 cut(s) | 973 |
| BshFI | GGCC | 5 cut(s) | 378, 452, 610, 912, 1998 |
| BshNI | GGYRCC | 1 cut(s) | 1925 |
| BsiHKAI | GWGCWC | 6 cut(s) | 415, 901, 986, 1030, 2233, 2277 |
| BsiHKCI | CYCGRG | 1 cut(s) | 294 |
| BsiSI | CCGG | 1 cut(s) | 1043 |
| BslFI | GGGAC | 2 cut(s) | 1117, 1228 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 629, 919, 1048, 1049, 1100, 1411, 1922, 2054, 2287 |
| BsmAI | GTCTC | 1 cut(s) | 1883 |
| BsmBI | CGTCTC | 1 cut(s) | 1883 |
| BsmFI | GGGAC | 2 cut(s) | 1117, 1228 |
| BsnI | GGCC | 5 cut(s) | 378, 452, 610, 912, 1998 |
| BsoBI | CYCGRG | 1 cut(s) | 294 |
| Bsp1286I | GDGCHC | 8 cut(s) | 415, 901, 986, 1030, 1958, 2233, 2277, 2293 |
| Bsp1407I | TGTACA | 2 cut(s) | 640, 1060 |
| Bsp19I | CCATGG | 2 cut(s) | 453, 1331 |
| BspACI | CCGC | 4 cut(s) | 971, 1639, 2317, 2521 |
| BspANI | GGCC | 5 cut(s) | 378, 452, 610, 912, 1998 |
| BspCNI | CTCAG | 5 cut(s) | 1144, 1820, 1833, 1930, 2317 |
| BspFNI | CGCG | 1 cut(s) | 973 |
| BspHI | TCATGA | 1 cut(s) | 1408 |
| BspLI | GGNNCC | 3 cut(s) | 1566, 1927, 2544 |
| BspMI | ACCTGC | 2 cut(s) | 1103, 1531 |
| BspOI | GCTAGC | 1 cut(s) | 84 |
| BspPI | GGATC | 6 cut(s) | 203, 225, 1607, 2503, 2537, 2550 |
| BspT107I | GGYRCC | 1 cut(s) | 1925 |
| BsrBI | CCGCTC | 1 cut(s) | 2317 |
| BsrGI | TGTACA | 2 cut(s) | 640, 1060 |
| BsrI | ACTGG | 5 cut(s) | 200, 233, 740, 2407, 2533 |
| BssECI | CCNNGG | 6 cut(s) | 453, 628, 1331, 2173, 2284, 2376 |
| BssNAI | GTATAC | 1 cut(s) | 948 |
| BssNI | GRCGYC | 1 cut(s) | 759 |
| BssT1I | CCWWGG | 6 cut(s) | 453, 628, 1331, 2173, 2284, 2376 |
| Bst1107I | GTATAC | 1 cut(s) | 948 |
| Bst2UI | CCWGG | 1 cut(s) | 1995 |
| Bst4CI | ACNGT | 9 cut(s) | 132, 202, 871, 1082, 1219, 1722, 1739, 1845, 1886 |
| Bst6I | CTCTTC | 2 cut(s) | 1893, 2509 |
| BstACI | GRCGYC | 1 cut(s) | 759 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 2127, 2259 |
| BstAUI | TGTACA | 2 cut(s) | 640, 1060 |
| BstBAI | YACGTR | 1 cut(s) | 1953 |
| BstC8I | GCNNGC | 7 cut(s) | 82, 275, 1552, 2102, 2119, 2123, 2188 |
| BstDEI | CTNAG | 8 cut(s) | 1152, 1439, 1576, 1674, 1807, 1820, 1917, 2325 |
| BstDSI | CCRYGG | 2 cut(s) | 453, 1331 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1409 |
| BstF5I | GGATG | 4 cut(s) | 1120, 1495, 1774, 2143 |
| BstFNI | CGCG | 1 cut(s) | 973 |
| BstH2I | RGCGCY | 1 cut(s) | 649 |
| BstHHI | GCGC | 2 cut(s) | 648, 1530 |
| BstMAI | GTCTC | 1 cut(s) | 1883 |
| BstNI | CCWGG | 1 cut(s) | 1995 |
| BstNSI | RCATGY | 6 cut(s) | 161, 1712, 1896, 2125, 2154, 2260 |
| BstSCI | CCNGG | 2 cut(s) | 1041, 1993 |
| BstSFI | CTRYAG | 2 cut(s) | 59, 495 |
| BstSLI | GKGCMC | 4 cut(s) | 415, 901, 1030, 1958 |
| BstUI | CGCG | 1 cut(s) | 973 |
| BstV1I | GCAGC | 4 cut(s) | 1056, 1666, 1717, 2065 |
| BstX2I | RGATCY | 3 cut(s) | 217, 1618, 2542 |
| BstYI | RGATCY | 3 cut(s) | 217, 1618, 2542 |
| BstZ17I | GTATAC | 1 cut(s) | 948 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1576 |
| BsuRI | GGCC | 5 cut(s) | 378, 452, 610, 912, 1998 |
| BtgI | CCRYGG | 2 cut(s) | 453, 1331 |
| BtsCI | GGATG | 4 cut(s) | 1120, 1495, 1774, 2143 |
| BtsIMutI | CAGTG | 3 cut(s) | 204, 876, 1074 |
| BveI | ACCTGC | 2 cut(s) | 1103, 1531 |
| Cac8I | GCNNGC | 7 cut(s) | 82, 275, 1552, 2102, 2119, 2123, 2188 |
| CaiI | CAGNNNCTG | 2 cut(s) | 206, 1069 |
| CciI | TCATGA | 1 cut(s) | 1408 |
| CfoI | GCGC | 2 cut(s) | 648, 1530 |
| Cfr13I | GGNCC | 4 cut(s) | 608, 911, 1402, 1997 |
| Csp6I | GTAC | 9 cut(s) | 92, 641, 692, 975, 1061, 1544, 1926, 2452, 2526 |
| CviQI | GTAC | 9 cut(s) | 92, 641, 692, 975, 1061, 1544, 1926, 2452, 2526 |
| DdeI | CTNAG | 8 cut(s) | 1152, 1439, 1576, 1674, 1807, 1820, 1917, 2325 |
| DraI | TTTAAA | 2 cut(s) | 136, 670 |
| DraIII | CACNNNGTG | 1 cut(s) | 1025 |
| EaeI | YGGCCR | 1 cut(s) | 376 |
| Eam1104I | CTCTTC | 2 cut(s) | 1893, 2509 |
| EarI | CTCTTC | 2 cut(s) | 1893, 2509 |
| Eco130I | CCWWGG | 6 cut(s) | 453, 628, 1331, 2173, 2284, 2376 |
| Eco24I | GRGCYC | 1 cut(s) | 2293 |
| Eco32I | GATATC | 2 cut(s) | 1636, 2169 |
| Eco47I | GGWCC | 1 cut(s) | 1402 |
| Eco47III | AGCGCT | 1 cut(s) | 647 |
| Eco57I | CTGAAG | 1 cut(s) | 710 |
| Eco81I | CCTNAGG | 1 cut(s) | 1576 |
| Eco88I | CYCGRG | 1 cut(s) | 294 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1409 |
| EcoRII | CCWGG | 1 cut(s) | 1993 |
| EcoRV | GATATC | 2 cut(s) | 1636, 2169 |
| EcoT14I | CCWWGG | 6 cut(s) | 453, 628, 1331, 2173, 2284, 2376 |
| EcoT22I | ATGCAT | 2 cut(s) | 1495, 1774 |
| EcoT38I | GRGCYC | 1 cut(s) | 2293 |
| ErhI | CCWWGG | 6 cut(s) | 453, 628, 1331, 2173, 2284, 2376 |
| Esp3I | CGTCTC | 1 cut(s) | 1883 |
| FaqI | GGGAC | 2 cut(s) | 1117, 1228 |
| FauI | CCCGC | 1 cut(s) | 2514 |
| FauNDI | CATATG | 1 cut(s) | 1520 |
| FbaI | TGATCA | 1 cut(s) | 1117 |
| FblI | GTMKAC | 4 cut(s) | 68, 947, 1840, 2155 |
| Fnu4HI | GCNGC | 4 cut(s) | 1070, 1655, 1706, 2079 |
| FokI | GGATG | 4 cut(s) | 1107, 1502, 1781, 2130 |
| FriOI | GRGCYC | 1 cut(s) | 2293 |
| Fsp4HI | GCNGC | 4 cut(s) | 1070, 1655, 1706, 2079 |
| FspBI | CTAG | 6 cut(s) | 81, 170, 731, 902, 2216, 2438 |
| GlaI | GCGC | 2 cut(s) | 647, 1529 |
| GluI | GCNGC | 4 cut(s) | 1070, 1655, 1706, 2079 |
| GsaI | CCCAGC | 2 cut(s) | 1166, 2108 |
| GsuI | CTGGAG | 3 cut(s) | 216, 1413, 2295 |
| HaeII | RGCGCY | 1 cut(s) | 649 |
| HaeIII | GGCC | 5 cut(s) | 378, 452, 610, 912, 1998 |
| HapII | CCGG | 1 cut(s) | 1043 |
| HhaI | GCGC | 2 cut(s) | 648, 1530 |
| Hin1I | GRCGYC | 1 cut(s) | 759 |
| Hin6I | GCGC | 2 cut(s) | 646, 1528 |
| HinP1I | GCGC | 2 cut(s) | 646, 1528 |
| HincII | GTYRAC | 1 cut(s) | 2156 |
| HindII | GTYRAC | 1 cut(s) | 2156 |
| HinfI | GANTC | 6 cut(s) | 4, 874, 1479, 1805, 2178, 2333 |
| HpaII | CCGG | 1 cut(s) | 1043 |
| HphI | GGTGA | 4 cut(s) | 1127, 1318, 1388, 1416 |
| Hpy188I | TCNGA | 5 cut(s) | 530, 829, 1727, 1920, 2471 |
| Hpy99I | CGWCG | 1 cut(s) | 764 |
| HpyAV | CCTTC | 9 cut(s) | 332, 442, 864, 1005, 1303, 1415, 1881, 2048, 2478 |
| HpyCH4III | ACNGT | 9 cut(s) | 132, 202, 871, 1082, 1219, 1722, 1739, 1845, 1886 |
| HpyCH4IV | ACGT | 5 cut(s) | 90, 665, 759, 1297, 1952 |
| HpyF3I | CTNAG | 8 cut(s) | 1152, 1439, 1576, 1674, 1807, 1820, 1917, 2325 |
| HpySE526I | ACGT | 5 cut(s) | 90, 665, 759, 1297, 1952 |
| Hsp92I | GRCGYC | 1 cut(s) | 759 |
| HspAI | GCGC | 2 cut(s) | 646, 1528 |
| KpnI | GGTACC | 1 cut(s) | 1929 |
| Ksp22I | TGATCA | 1 cut(s) | 1117 |
| LmnI | GCTCC | 6 cut(s) | 235, 739, 1991, 2075, 2236, 2280 |
| Lsp1109I | GCAGC | 4 cut(s) | 1056, 1666, 1717, 2065 |
| LweI | GCATC | 7 cut(s) | 490, 577, 1099, 1162, 1480, 1502, 1759 |
| MaeI | CTAG | 6 cut(s) | 81, 170, 731, 902, 2216, 2438 |
| MaeII | ACGT | 5 cut(s) | 90, 665, 759, 1297, 1952 |
| MaeIII | GTNAC | 9 cut(s) | 139, 202, 305, 427, 743, 814, 1129, 2330, 2424 |
| MbiI | CCGCTC | 1 cut(s) | 2317 |
| MfeI | CAATTG | 1 cut(s) | 1263 |
| MflI | RGATCY | 3 cut(s) | 217, 1618, 2542 |
| MhlI | GDGCHC | 8 cut(s) | 415, 901, 986, 1030, 1958, 2233, 2277, 2293 |
| MlsI | TGGCCA | 1 cut(s) | 378 |
| MluNI | TGGCCA | 1 cut(s) | 378 |
| MlyI | GAGTC | 3 cut(s) | 1799, 2187, 2327 |
| MmeI | TCCRAC | 3 cut(s) | 508, 1592, 1750 |
| Mox20I | TGGCCA | 1 cut(s) | 378 |
| Mph1103I | ATGCAT | 2 cut(s) | 1495, 1774 |
| MscI | TGGCCA | 1 cut(s) | 378 |
| MseI | TTAA | 8 cut(s) | 135, 281, 669, 864, 1512, 1932, 1979, 2358 |
| MslI | CAYNNNNRTG | 3 cut(s) | 384, 1695, 2348 |
| Msp20I | TGGCCA | 1 cut(s) | 378 |
| MspI | CCGG | 1 cut(s) | 1043 |
| MspR9I | CCNGG | 2 cut(s) | 1043, 1995 |
| MssI | GTTTAAAC | 1 cut(s) | 670 |
| MunI | CAATTG | 1 cut(s) | 1263 |
| MvaI | CCWGG | 1 cut(s) | 1995 |
| MvnI | CGCG | 1 cut(s) | 973 |
| NciI | CCSGG | 1 cut(s) | 1043 |
| NcoI | CCATGG | 2 cut(s) | 453, 1331 |
| NdeI | CATATG | 1 cut(s) | 1520 |
| NheI | GCTAGC | 1 cut(s) | 80 |
| NlaIV | GGNNCC | 3 cut(s) | 1566, 1927, 2544 |
| NmuCI | GTSAC | 4 cut(s) | 139, 202, 814, 2330 |
| NsiI | ATGCAT | 2 cut(s) | 1495, 1774 |
| NspI | RCATGY | 6 cut(s) | 161, 1712, 1896, 2125, 2154, 2260 |
| PaeI | GCATGC | 1 cut(s) | 2125 |
| PagI | TCATGA | 1 cut(s) | 1408 |
| PaqCI | CACCTGC | 1 cut(s) | 1103 |
| PciI | ACATGT | 2 cut(s) | 2150, 2256 |
| PfeI | GAWTC | 3 cut(s) | 4, 874, 1479 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1100 |
| PkrI | GCNGC | 4 cut(s) | 1071, 1656, 1707, 2080 |
| PleI | GAGTC | 3 cut(s) | 1799, 2186, 2327 |
| PmeI | GTTTAAAC | 1 cut(s) | 670 |
| PpsI | GAGTC | 3 cut(s) | 1799, 2186, 2327 |
| Ppu21I | YACGTR | 1 cut(s) | 1953 |
| PscI | ACATGT | 2 cut(s) | 2150, 2256 |
| PshBI | ATTAAT | 1 cut(s) | 1979 |
| Psp6I | CCWGG | 1 cut(s) | 1993 |
| PspFI | CCCAGC | 2 cut(s) | 1162, 2104 |
| PspGI | CCWGG | 1 cut(s) | 1993 |
| PspN4I | GGNNCC | 3 cut(s) | 1566, 1927, 2544 |
| PspPI | GGNCC | 4 cut(s) | 608, 911, 1402, 1997 |
| PstNI | CAGNNNCTG | 2 cut(s) | 206, 1069 |
| PsuI | RGATCY | 3 cut(s) | 217, 1618, 2542 |
| RsaI | GTAC | 9 cut(s) | 93, 642, 693, 976, 1062, 1545, 1927, 2453, 2527 |
| RsaNI | GTAC | 9 cut(s) | 92, 641, 692, 975, 1061, 1544, 1926, 2452, 2526 |
| RseI | CAYNNNNRTG | 3 cut(s) | 384, 1695, 2348 |
| SalI | GTCGAC | 1 cut(s) | 2154 |
| SaqAI | TTAA | 8 cut(s) | 135, 281, 669, 864, 1512, 1932, 1979, 2358 |
| SatI | GCNGC | 4 cut(s) | 1070, 1655, 1706, 2079 |
| Sau96I | GGNCC | 4 cut(s) | 608, 911, 1402, 1997 |
| SchI | GAGTC | 3 cut(s) | 1799, 2187, 2327 |
| ScrFI | CCNGG | 2 cut(s) | 1043, 1995 |
| SduI | GDGCHC | 8 cut(s) | 415, 901, 986, 1030, 1958, 2233, 2277, 2293 |
| SfaNI | GCATC | 7 cut(s) | 490, 577, 1099, 1162, 1480, 1502, 1759 |
| SfcI | CTRYAG | 2 cut(s) | 59, 495 |
| SinI | GGWCC | 1 cut(s) | 1402 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 384, 1695, 2348 |
| SmlI | CTYRAG | 1 cut(s) | 951 |
| SmoI | CTYRAG | 1 cut(s) | 951 |
| SpeI | ACTAGT | 1 cut(s) | 2437 |
| SphI | GCATGC | 1 cut(s) | 2125 |
| SsiI | CCGC | 4 cut(s) | 971, 1639, 2317, 2521 |
| SspMI | CTAG | 6 cut(s) | 81, 170, 731, 902, 2216, 2438 |
| StyD4I | CCNGG | 2 cut(s) | 1041, 1993 |
| StyI | CCWWGG | 6 cut(s) | 453, 628, 1331, 2173, 2284, 2376 |
| TaaI | ACNGT | 9 cut(s) | 132, 202, 871, 1082, 1219, 1722, 1739, 1845, 1886 |
| TaiI | ACGT | 5 cut(s) | 93, 668, 762, 1300, 1955 |
| TaqI | TCGA | 6 cut(s) | 505, 585, 762, 2061, 2155, 2336 |
| TatI | WGTACW | 2 cut(s) | 640, 1060 |
| TfiI | GAWTC | 3 cut(s) | 4, 874, 1479 |
| Tru1I | TTAA | 8 cut(s) | 135, 281, 669, 864, 1512, 1932, 1979, 2358 |
| Tru9I | TTAA | 8 cut(s) | 135, 281, 669, 864, 1512, 1932, 1979, 2358 |
| TscAI | CASTG | 3 cut(s) | 211, 876, 1081 |
| TseFI | GTSAC | 4 cut(s) | 139, 202, 814, 2330 |
| TseI | GCWGC | 4 cut(s) | 1069, 1654, 1705, 2078 |
| Tsp45I | GTSAC | 4 cut(s) | 139, 202, 814, 2330 |
| TspGWI | ACGGA | 4 cut(s) | 101, 1502, 2469, 2549 |
| TspRI | CASTG | 3 cut(s) | 211, 876, 1081 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1100 |
| VneI | GTGCAC | 3 cut(s) | 411, 897, 1026 |
| VpaK11BI | GGWCC | 1 cut(s) | 1402 |
| VspI | ATTAAT | 1 cut(s) | 1979 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1409 |
| XapI | RAATTY | 3 cut(s) | 98, 680, 1256 |
| XbaI | TCTAGA | 2 cut(s) | 169, 2215 |
| XceI | RCATGY | 6 cut(s) | 161, 1712, 1896, 2125, 2154, 2260 |
| XmiI | GTMKAC | 4 cut(s) | 68, 947, 1840, 2155 |
| XspI | CTAG | 6 cut(s) | 81, 170, 731, 902, 2216, 2438 |
| ZraI | GACGTC | 1 cut(s) | 760 |
| Zsp2I | ATGCAT | 2 cut(s) | 1495, 1774 |