Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G32000 AT2G32000 |
| fragaria_vesca | FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 |
| malus_domestica | MD04G1023600.v1.1 |
| prunus_persica | Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 |
| pyrus_communis | pycom04g02020 |
| rosa_chinensis | RchiOBHm_Chr4g0412031 |
| rosa_laevigata | RLG00000008327 |
| rosa_multiflora | Rmu_co8041726.1_g000001 Rmu_sc0000711.1_g000051 |
| rosa_roxburghii | Rroxscaffold_1G00042480 Rroxscaffold_5G00346360 Rroxscaffold_5G00356250 Rroxscaffold_5G00356340 |
| rosa_rugosa | Rorug04G0106700 Rorug04G0106800 Rorug04G0106900 |
| rosa_samantha | Rh4AG171100 Rh4BG168800 Rh4BG169700 Rh4CG181900 Rh4DG164200 Rh4DG165300 |
| rosa_wichuraiana | Rw0G007770 Rw0G008800 Rw4G014260 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1127 |
| Acc36I | ACCTGC | 1 cut(s) | 1127 |
| Acc65I | GGTACC | 1 cut(s) | 1943 |
| AccB1I | GGYRCC | 1 cut(s) | 1943 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1124 |
| AccBSI | CCGCTC | 1 cut(s) | 2335 |
| AccI | GTMKAC | 4 cut(s) | 92, 971, 1858, 2173 |
| AccII | CGCG | 2 cut(s) | 81, 997 |
| AciI | CCGC | 6 cut(s) | 79, 81, 995, 1657, 2335, 2539 |
| AclI | AACGTT | 1 cut(s) | 69 |
| AclWI | GGATC | 6 cut(s) | 227, 249, 1625, 2521, 2555, 2568 |
| AcoI | YGGCCR | 1 cut(s) | 400 |
| AcsI | RAATTY | 3 cut(s) | 122, 704, 1280 |
| AcuI | CTGAAG | 1 cut(s) | 734 |
| AdeI | CACNNNGTG | 1 cut(s) | 1049 |
| AfaI | GTAC | 9 cut(s) | 117, 153, 666, 717, 1000, 1086, 1563, 1945, 2545 |
| AfeI | AGCGCT | 1 cut(s) | 671 |
| AflIII | ACRYGT | 3 cut(s) | 1321, 2168, 2274 |
| AhlI | ACTAGT | 1 cut(s) | 2455 |
| AjnI | CCWGG | 2 cut(s) | 2011, 2017 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1272, 1304 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2214, 2246 |
| Alw21I | GWGCWC | 6 cut(s) | 439, 925, 1010, 1054, 2251, 2295 |
| Alw26I | GTCTC | 1 cut(s) | 1901 |
| Alw44I | GTGCAC | 3 cut(s) | 435, 921, 1050 |
| AlwI | GGATC | 6 cut(s) | 227, 249, 1625, 2521, 2555, 2568 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 230, 1093 |
| Ama87I | CYCGRG | 1 cut(s) | 318 |
| Aor51HI | AGCGCT | 1 cut(s) | 671 |
| AoxI | GGCC | 5 cut(s) | 400, 474, 632, 934, 2014 |
| ApaLI | GTGCAC | 3 cut(s) | 435, 921, 1050 |
| ApeKI | GCWGC | 4 cut(s) | 1093, 1672, 1723, 2096 |
| ApoI | RAATTY | 3 cut(s) | 122, 704, 1280 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2072, 2104 |
| AseI | ATTAAT | 1 cut(s) | 1997 |
| Asp718I | GGTACC | 1 cut(s) | 1943 |
| AspLEI | GCGC | 3 cut(s) | 13, 672, 1554 |
| AspS9I | GGNCC | 4 cut(s) | 632, 935, 1426, 2015 |
| AsuC2I | CCSGG | 1 cut(s) | 1067 |
| AsuHPI | GGTGA | 3 cut(s) | 1151, 1342, 1440 |
| AsuNHI | GCTAGC | 1 cut(s) | 104 |
| AvaI | CYCGRG | 1 cut(s) | 318 |
| AvaII | GGWCC | 1 cut(s) | 1426 |
| AxyI | CCTNAGG | 1 cut(s) | 1594 |
| BaeGI | GKGCMC | 3 cut(s) | 439, 925, 1054 |
| BalI | TGGCCA | 1 cut(s) | 402 |
| BamHI | GGATCC | 1 cut(s) | 2560 |
| BanI | GGYRCC | 1 cut(s) | 1943 |
| BanII | GRGCYC | 1 cut(s) | 2311 |
| Bbv12I | GWGCWC | 6 cut(s) | 439, 925, 1010, 1054, 2251, 2295 |
| BbvI | GCAGC | 4 cut(s) | 1080, 1684, 1735, 2083 |
| BccI | CCATC | 2 cut(s) | 818, 2396 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2250, 2284 |
| BciT130I | CCWGG | 2 cut(s) | 2013, 2019 |
| BclI | TGATCA | 1 cut(s) | 1141 |
| BcnI | CCSGG | 1 cut(s) | 1067 |
| BcoDI | GTCTC | 1 cut(s) | 1901 |
| BcuI | ACTAGT | 1 cut(s) | 2455 |
| BfaI | CTAG | 6 cut(s) | 23, 105, 755, 926, 2234, 2456 |
| BfmI | CTRYAG | 1 cut(s) | 519 |
| BfoI | RGCGCY | 1 cut(s) | 673 |
| BfuAI | ACCTGC | 1 cut(s) | 1127 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2022 |
| BglII | AGATCT | 1 cut(s) | 1636 |
| BisI | GCNGC | 4 cut(s) | 1094, 1673, 1724, 2097 |
| BlsI | GCNGC | 4 cut(s) | 1095, 1674, 1725, 2098 |
| Bme1390I | CCNGG | 3 cut(s) | 1067, 2013, 2019 |
| Bme18I | GGWCC | 1 cut(s) | 1426 |
| BmeT110I | CYCGRG | 1 cut(s) | 318 |
| BmgT120I | GGNCC | 4 cut(s) | 632, 935, 1426, 2015 |
| BmiI | GGNNCC | 3 cut(s) | 1584, 1945, 2562 |
| BmrFI | CCNGG | 3 cut(s) | 1067, 2013, 2019 |
| BmrI | ACTGGG | 2 cut(s) | 769, 2556 |
| BmsI | GCATC | 7 cut(s) | 514, 601, 1123, 1186, 1504, 1526, 1777 |
| BmtI | GCTAGC | 1 cut(s) | 108 |
| BmuI | ACTGGG | 2 cut(s) | 769, 2556 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2336, 2368 |
| BpmI | CTGGAG | 3 cut(s) | 240, 1437, 2313 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1692 |
| BpuEI | CTTGAG | 1 cut(s) | 960 |
| BpuMI | CCSGG | 1 cut(s) | 1067 |
| BsaBI | GATNNNNATC | 2 cut(s) | 354, 1635 |
| BsaJI | CCNNGG | 8 cut(s) | 79, 477, 652, 1355, 2017, 2191, 2302, 2394 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 59, 89, 1231, 1261, 1435, 1465, 2453, 2483 |
| Bse1I | ACTGG | 5 cut(s) | 224, 257, 764, 2425, 2551 |
| Bse21I | CCTNAGG | 1 cut(s) | 1594 |
| Bse8I | GATNNNNATC | 2 cut(s) | 354, 1635 |
| BseBI | CCWGG | 2 cut(s) | 2013, 2019 |
| BseDI | CCNNGG | 8 cut(s) | 79, 477, 652, 1355, 2017, 2191, 2302, 2394 |
| BseGI | GGATG | 5 cut(s) | 1138, 1144, 1519, 1792, 2161 |
| BseJI | GATNNNNATC | 2 cut(s) | 354, 1635 |
| BseMII | CTCAG | 5 cut(s) | 1167, 1839, 1852, 1949, 2334 |
| BseNI | ACTGG | 5 cut(s) | 224, 257, 764, 2425, 2551 |
| BseRI | GAGGAG | 1 cut(s) | 2285 |
| BseSI | GKGCMC | 3 cut(s) | 439, 925, 1054 |
| BseXI | GCAGC | 4 cut(s) | 1080, 1684, 1735, 2083 |
| BseYI | CCCAGC | 3 cut(s) | 44, 1186, 2122 |
| BsgI | GTGCAG | 1 cut(s) | 1742 |
| Bsh1236I | CGCG | 2 cut(s) | 81, 997 |
| BshFI | GGCC | 5 cut(s) | 402, 476, 634, 936, 2016 |
| BshNI | GGYRCC | 1 cut(s) | 1943 |
| BsiHKAI | GWGCWC | 6 cut(s) | 439, 925, 1010, 1054, 2251, 2295 |
| BsiHKCI | CYCGRG | 1 cut(s) | 318 |
| BsiSI | CCGG | 1 cut(s) | 1067 |
| BslFI | GGGAC | 2 cut(s) | 1141, 1252 |
| BsmAI | GTCTC | 1 cut(s) | 1901 |
| BsmBI | CGTCTC | 1 cut(s) | 1901 |
| BsmFI | GGGAC | 2 cut(s) | 1141, 1252 |
| BsnI | GGCC | 5 cut(s) | 402, 476, 634, 936, 2016 |
| BsoBI | CYCGRG | 1 cut(s) | 318 |
| Bsp1286I | GDGCHC | 7 cut(s) | 439, 925, 1010, 1054, 2251, 2295, 2311 |
| Bsp1407I | TGTACA | 4 cut(s) | 151, 664, 1084, 1561 |
| Bsp19I | CCATGG | 2 cut(s) | 477, 1355 |
| BspACI | CCGC | 6 cut(s) | 79, 81, 995, 1657, 2335, 2539 |
| BspANI | GGCC | 5 cut(s) | 402, 476, 634, 936, 2016 |
| BspCNI | CTCAG | 5 cut(s) | 1168, 1838, 1851, 1948, 2335 |
| BspFNI | CGCG | 2 cut(s) | 81, 997 |
| BspHI | TCATGA | 1 cut(s) | 1432 |
| BspLI | GGNNCC | 3 cut(s) | 1584, 1945, 2562 |
| BspMI | ACCTGC | 1 cut(s) | 1127 |
| BspOI | GCTAGC | 1 cut(s) | 108 |
| BspPI | GGATC | 6 cut(s) | 227, 249, 1625, 2521, 2555, 2568 |
| BspT107I | GGYRCC | 1 cut(s) | 1943 |
| BsrBI | CCGCTC | 1 cut(s) | 2335 |
| BsrGI | TGTACA | 4 cut(s) | 151, 664, 1084, 1561 |
| BsrI | ACTGG | 5 cut(s) | 224, 257, 764, 2425, 2551 |
| BssECI | CCNNGG | 8 cut(s) | 79, 477, 652, 1355, 2017, 2191, 2302, 2394 |
| BssNAI | GTATAC | 1 cut(s) | 972 |
| BssT1I | CCWWGG | 6 cut(s) | 477, 652, 1355, 2191, 2302, 2394 |
| Bst1107I | GTATAC | 1 cut(s) | 972 |
| Bst2UI | CCWGG | 2 cut(s) | 2013, 2019 |
| Bst4CI | ACNGT | 9 cut(s) | 156, 226, 895, 1106, 1243, 1740, 1757, 1863, 1904 |
| Bst6I | CTCTTC | 2 cut(s) | 1911, 2527 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 2145, 2277 |
| BstAUI | TGTACA | 4 cut(s) | 151, 664, 1084, 1561 |
| BstC8I | GCNNGC | 8 cut(s) | 15, 106, 299, 1570, 2120, 2137, 2141, 2206 |
| BstDEI | CTNAG | 8 cut(s) | 1176, 1463, 1594, 1692, 1825, 1838, 1935, 2343 |
| BstDSI | CCRYGG | 3 cut(s) | 79, 477, 1355 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1433, 2016 |
| BstF5I | GGATG | 5 cut(s) | 1138, 1144, 1519, 1792, 2161 |
| BstFNI | CGCG | 2 cut(s) | 81, 997 |
| BstH2I | RGCGCY | 1 cut(s) | 673 |
| BstHHI | GCGC | 3 cut(s) | 13, 672, 1554 |
| BstMAI | GTCTC | 1 cut(s) | 1901 |
| BstNI | CCWGG | 2 cut(s) | 2013, 2019 |
| BstNSI | RCATGY | 7 cut(s) | 185, 1730, 1914, 1974, 2143, 2172, 2278 |
| BstSCI | CCNGG | 3 cut(s) | 1065, 2011, 2017 |
| BstSFI | CTRYAG | 1 cut(s) | 519 |
| BstSLI | GKGCMC | 3 cut(s) | 439, 925, 1054 |
| BstUI | CGCG | 2 cut(s) | 81, 997 |
| BstV1I | GCAGC | 4 cut(s) | 1080, 1684, 1735, 2083 |
| BstX2I | RGATCY | 3 cut(s) | 241, 1636, 2560 |
| BstYI | RGATCY | 3 cut(s) | 241, 1636, 2560 |
| BstZ17I | GTATAC | 1 cut(s) | 972 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1594 |
| BsuRI | GGCC | 5 cut(s) | 402, 476, 634, 936, 2016 |
| BtgI | CCRYGG | 3 cut(s) | 79, 477, 1355 |
| BtsCI | GGATG | 5 cut(s) | 1138, 1144, 1519, 1792, 2161 |
| BtsIMutI | CAGTG | 3 cut(s) | 228, 900, 1098 |
| BveI | ACCTGC | 1 cut(s) | 1127 |
| Cac8I | GCNNGC | 8 cut(s) | 15, 106, 299, 1570, 2120, 2137, 2141, 2206 |
| CaiI | CAGNNNCTG | 2 cut(s) | 230, 1093 |
| CciI | TCATGA | 1 cut(s) | 1432 |
| CfoI | GCGC | 3 cut(s) | 13, 672, 1554 |
| Cfr13I | GGNCC | 4 cut(s) | 632, 935, 1426, 2015 |
| Cfr42I | CCGCGG | 1 cut(s) | 82 |
| Csp6I | GTAC | 9 cut(s) | 116, 152, 665, 716, 999, 1085, 1562, 1944, 2544 |
| CviQI | GTAC | 9 cut(s) | 116, 152, 665, 716, 999, 1085, 1562, 1944, 2544 |
| DdeI | CTNAG | 8 cut(s) | 1176, 1463, 1594, 1692, 1825, 1838, 1935, 2343 |
| DraI | TTTAAA | 2 cut(s) | 160, 694 |
| DraIII | CACNNNGTG | 1 cut(s) | 1049 |
| EaeI | YGGCCR | 1 cut(s) | 400 |
| Eam1104I | CTCTTC | 2 cut(s) | 1911, 2527 |
| EarI | CTCTTC | 2 cut(s) | 1911, 2527 |
| Eco130I | CCWWGG | 6 cut(s) | 477, 652, 1355, 2191, 2302, 2394 |
| Eco24I | GRGCYC | 1 cut(s) | 2311 |
| Eco32I | GATATC | 2 cut(s) | 1654, 2187 |
| Eco47I | GGWCC | 1 cut(s) | 1426 |
| Eco47III | AGCGCT | 1 cut(s) | 671 |
| Eco57I | CTGAAG | 1 cut(s) | 734 |
| Eco81I | CCTNAGG | 1 cut(s) | 1594 |
| Eco88I | CYCGRG | 1 cut(s) | 318 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1433, 2016 |
| EcoRII | CCWGG | 2 cut(s) | 2011, 2017 |
| EcoRV | GATATC | 2 cut(s) | 1654, 2187 |
| EcoT14I | CCWWGG | 6 cut(s) | 477, 652, 1355, 2191, 2302, 2394 |
| EcoT22I | ATGCAT | 2 cut(s) | 1519, 1792 |
| EcoT38I | GRGCYC | 1 cut(s) | 2311 |
| ErhI | CCWWGG | 6 cut(s) | 477, 652, 1355, 2191, 2302, 2394 |
| Esp3I | CGTCTC | 1 cut(s) | 1901 |
| FaqI | GGGAC | 2 cut(s) | 1141, 1252 |
| FauI | CCCGC | 2 cut(s) | 86, 2532 |
| FauNDI | CATATG | 1 cut(s) | 1544 |
| FbaI | TGATCA | 1 cut(s) | 1141 |
| FblI | GTMKAC | 4 cut(s) | 92, 971, 1858, 2173 |
| Fnu4HI | GCNGC | 4 cut(s) | 1094, 1673, 1724, 2097 |
| FokI | GGATG | 5 cut(s) | 1131, 1145, 1526, 1799, 2148 |
| FriOI | GRGCYC | 1 cut(s) | 2311 |
| Fsp4HI | GCNGC | 4 cut(s) | 1094, 1673, 1724, 2097 |
| FspBI | CTAG | 6 cut(s) | 23, 105, 755, 926, 2234, 2456 |
| GlaI | GCGC | 3 cut(s) | 12, 671, 1553 |
| GluI | GCNGC | 4 cut(s) | 1094, 1673, 1724, 2097 |
| GsaI | CCCAGC | 3 cut(s) | 48, 1190, 2126 |
| GsuI | CTGGAG | 3 cut(s) | 240, 1437, 2313 |
| HaeII | RGCGCY | 1 cut(s) | 673 |
| HaeIII | GGCC | 5 cut(s) | 402, 476, 634, 936, 2016 |
| HapII | CCGG | 1 cut(s) | 1067 |
| HhaI | GCGC | 3 cut(s) | 13, 672, 1554 |
| Hin6I | GCGC | 3 cut(s) | 11, 670, 1552 |
| HinP1I | GCGC | 3 cut(s) | 11, 670, 1552 |
| HincII | GTYRAC | 1 cut(s) | 2174 |
| HindII | GTYRAC | 1 cut(s) | 2174 |
| HinfI | GANTC | 5 cut(s) | 144, 898, 1503, 1823, 2351 |
| HpaII | CCGG | 1 cut(s) | 1067 |
| HphI | GGTGA | 3 cut(s) | 1151, 1342, 1440 |
| Hpy188I | TCNGA | 5 cut(s) | 554, 853, 1745, 1938, 2489 |
| HpyCH4III | ACNGT | 9 cut(s) | 156, 226, 895, 1106, 1243, 1740, 1757, 1863, 1904 |
| HpyCH4IV | ACGT | 3 cut(s) | 69, 114, 1321 |
| HpyF3I | CTNAG | 8 cut(s) | 1176, 1463, 1594, 1692, 1825, 1838, 1935, 2343 |
| HpySE526I | ACGT | 3 cut(s) | 69, 114, 1321 |
| HspAI | GCGC | 3 cut(s) | 11, 670, 1552 |
| KpnI | GGTACC | 1 cut(s) | 1947 |
| Ksp22I | TGATCA | 1 cut(s) | 1141 |
| KspI | CCGCGG | 1 cut(s) | 82 |
| LmnI | GCTCC | 6 cut(s) | 259, 763, 2009, 2093, 2254, 2298 |
| Lsp1109I | GCAGC | 4 cut(s) | 1080, 1684, 1735, 2083 |
| LweI | GCATC | 7 cut(s) | 514, 601, 1123, 1186, 1504, 1526, 1777 |
| MaeI | CTAG | 6 cut(s) | 23, 105, 755, 926, 2234, 2456 |
| MaeII | ACGT | 3 cut(s) | 69, 114, 1321 |
| MaeIII | GTNAC | 9 cut(s) | 163, 226, 329, 451, 767, 838, 1153, 2348, 2442 |
| MbiI | CCGCTC | 1 cut(s) | 2335 |
| MfeI | CAATTG | 1 cut(s) | 1287 |
| MflI | RGATCY | 3 cut(s) | 241, 1636, 2560 |
| MhlI | GDGCHC | 7 cut(s) | 439, 925, 1010, 1054, 2251, 2295, 2311 |
| MlsI | TGGCCA | 1 cut(s) | 402 |
| MluNI | TGGCCA | 1 cut(s) | 402 |
| MlyI | GAGTC | 2 cut(s) | 1817, 2345 |
| MmeI | TCCRAC | 3 cut(s) | 532, 1610, 1768 |
| Mox20I | TGGCCA | 1 cut(s) | 402 |
| Mph1103I | ATGCAT | 2 cut(s) | 1519, 1792 |
| MscI | TGGCCA | 1 cut(s) | 402 |
| MseI | TTAA | 8 cut(s) | 159, 305, 693, 888, 1536, 1950, 1997, 2376 |
| MslI | CAYNNNNRTG | 3 cut(s) | 408, 1713, 2366 |
| Msp20I | TGGCCA | 1 cut(s) | 402 |
| MspA1I | CMGCKG | 1 cut(s) | 81 |
| MspI | CCGG | 1 cut(s) | 1067 |
| MspR9I | CCNGG | 3 cut(s) | 1067, 2013, 2019 |
| MunI | CAATTG | 1 cut(s) | 1287 |
| MvaI | CCWGG | 2 cut(s) | 2013, 2019 |
| MvnI | CGCG | 2 cut(s) | 81, 997 |
| NciI | CCSGG | 1 cut(s) | 1067 |
| NcoI | CCATGG | 2 cut(s) | 477, 1355 |
| NdeI | CATATG | 1 cut(s) | 1544 |
| NheI | GCTAGC | 1 cut(s) | 104 |
| NlaIV | GGNNCC | 3 cut(s) | 1584, 1945, 2562 |
| NmeAIII | GCCGAG | 1 cut(s) | 61 |
| NmuCI | GTSAC | 4 cut(s) | 163, 226, 838, 2348 |
| NsiI | ATGCAT | 2 cut(s) | 1519, 1792 |
| NspI | RCATGY | 7 cut(s) | 185, 1730, 1914, 1974, 2143, 2172, 2278 |
| PaeI | GCATGC | 1 cut(s) | 2143 |
| PagI | TCATGA | 1 cut(s) | 1432 |
| PaqCI | CACCTGC | 1 cut(s) | 1127 |
| PciI | ACATGT | 2 cut(s) | 2168, 2274 |
| PfeI | GAWTC | 3 cut(s) | 144, 898, 1503 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1124 |
| PkrI | GCNGC | 4 cut(s) | 1095, 1674, 1725, 2098 |
| PleI | GAGTC | 2 cut(s) | 1817, 2345 |
| PpsI | GAGTC | 2 cut(s) | 1817, 2345 |
| PscI | ACATGT | 2 cut(s) | 2168, 2274 |
| PshBI | ATTAAT | 1 cut(s) | 1997 |
| Psp1406I | AACGTT | 1 cut(s) | 69 |
| Psp6I | CCWGG | 2 cut(s) | 2011, 2017 |
| PspFI | CCCAGC | 3 cut(s) | 44, 1186, 2122 |
| PspGI | CCWGG | 2 cut(s) | 2011, 2017 |
| PspN4I | GGNNCC | 3 cut(s) | 1584, 1945, 2562 |
| PspPI | GGNCC | 4 cut(s) | 632, 935, 1426, 2015 |
| PstNI | CAGNNNCTG | 2 cut(s) | 230, 1093 |
| PsuI | RGATCY | 3 cut(s) | 241, 1636, 2560 |
| RsaI | GTAC | 9 cut(s) | 117, 153, 666, 717, 1000, 1086, 1563, 1945, 2545 |
| RsaNI | GTAC | 9 cut(s) | 116, 152, 665, 716, 999, 1085, 1562, 1944, 2544 |
| RseI | CAYNNNNRTG | 3 cut(s) | 408, 1713, 2366 |
| SacII | CCGCGG | 1 cut(s) | 82 |
| SalI | GTCGAC | 1 cut(s) | 2172 |
| SaqAI | TTAA | 8 cut(s) | 159, 305, 693, 888, 1536, 1950, 1997, 2376 |
| SatI | GCNGC | 4 cut(s) | 1094, 1673, 1724, 2097 |
| Sau96I | GGNCC | 4 cut(s) | 632, 935, 1426, 2015 |
| SchI | GAGTC | 2 cut(s) | 1817, 2345 |
| ScrFI | CCNGG | 3 cut(s) | 1067, 2013, 2019 |
| SduI | GDGCHC | 7 cut(s) | 439, 925, 1010, 1054, 2251, 2295, 2311 |
| SfaNI | GCATC | 7 cut(s) | 514, 601, 1123, 1186, 1504, 1526, 1777 |
| SfcI | CTRYAG | 1 cut(s) | 519 |
| Sfr303I | CCGCGG | 1 cut(s) | 82 |
| SgrBI | CCGCGG | 1 cut(s) | 82 |
| SinI | GGWCC | 1 cut(s) | 1426 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 408, 1713, 2366 |
| SmlI | CTYRAG | 1 cut(s) | 975 |
| SmoI | CTYRAG | 1 cut(s) | 975 |
| SpeI | ACTAGT | 1 cut(s) | 2455 |
| SphI | GCATGC | 1 cut(s) | 2143 |
| SsiI | CCGC | 6 cut(s) | 79, 81, 995, 1657, 2335, 2539 |
| SspMI | CTAG | 6 cut(s) | 23, 105, 755, 926, 2234, 2456 |
| StyD4I | CCNGG | 3 cut(s) | 1065, 2011, 2017 |
| StyI | CCWWGG | 6 cut(s) | 477, 652, 1355, 2191, 2302, 2394 |
| TaaI | ACNGT | 9 cut(s) | 156, 226, 895, 1106, 1243, 1740, 1757, 1863, 1904 |
| TaiI | ACGT | 3 cut(s) | 72, 117, 1324 |
| TaqI | TCGA | 6 cut(s) | 529, 609, 786, 2079, 2173, 2354 |
| TatI | WGTACW | 4 cut(s) | 151, 664, 1084, 1561 |
| TfiI | GAWTC | 3 cut(s) | 144, 898, 1503 |
| Tru1I | TTAA | 8 cut(s) | 159, 305, 693, 888, 1536, 1950, 1997, 2376 |
| Tru9I | TTAA | 8 cut(s) | 159, 305, 693, 888, 1536, 1950, 1997, 2376 |
| TscAI | CASTG | 3 cut(s) | 235, 900, 1105 |
| TseFI | GTSAC | 4 cut(s) | 163, 226, 838, 2348 |
| TseI | GCWGC | 4 cut(s) | 1093, 1672, 1723, 2096 |
| Tsp45I | GTSAC | 4 cut(s) | 163, 226, 838, 2348 |
| TspGWI | ACGGA | 4 cut(s) | 125, 1145, 1526, 2567 |
| TspRI | CASTG | 3 cut(s) | 235, 900, 1105 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1124 |
| VneI | GTGCAC | 3 cut(s) | 435, 921, 1050 |
| VpaK11BI | GGWCC | 1 cut(s) | 1426 |
| VspI | ATTAAT | 1 cut(s) | 1997 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1433, 2016 |
| XapI | RAATTY | 3 cut(s) | 122, 704, 1280 |
| XbaI | TCTAGA | 1 cut(s) | 2233 |
| XceI | RCATGY | 7 cut(s) | 185, 1730, 1914, 1974, 2143, 2172, 2278 |
| XmiI | GTMKAC | 4 cut(s) | 92, 971, 1858, 2173 |
| XspI | CTAG | 6 cut(s) | 23, 105, 755, 926, 2234, 2456 |
| Zsp2I | ATGCAT | 2 cut(s) | 1519, 1792 |