Rh4DG164200

Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
Physical Location & Seq
Reverse (-)
32647650 .. 32658593
10944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG164200.1

Sequence Viewer

Length: 708 bp
ATGGATCCTTATTTACTGGCTATCATTTTCATGGATGCCCATAATGGCAAAGACAAATTTAATCATGCTTTGCTCTACAAGGAACTTACATGCCCTCTGGATAACTTTGAGCTATTAATCTGCTCCATGCCTGGCCCAGGTGGCAAGTCATTTTCTCTTTGCCCTTACTGCTACAATAGTCCTCCATTTGAAGGTATCGACACTCTCTTTGGAGCTGCAAAATCTGGTTCATCTGGCAAGCTGGGAAAGGGGGCTGGCATGCCATGCTTCCTTTGCCCGCATCCCACATGTCGAAATTCATTGATATCCCAAGGAGTCTGTGCTTGCCCTGAATGTAGTGGAACTCTTGTTCTAGACCCCGTAAGTGCTCCCAAGTGGCGACTATGTTGCAACATGTGCAATTTCCTTGTGCTCCTCCCCCAAGGGGCTCATCAAATCTCCACTACTCCAGAGCGGTGTCCTGAGTGTGACTCGACAATCATAGAAGTGGACTTTAATAAAAAGACAACACCCTTGGAAGATGGGGCGACCCAATATACTGGTTGTATTCTTTGTGATGAGTTACTTCATTCACTAGTGGAGATGAAGTACGGAAAGTCCTTTTTCAGAGGTTCAAGAGGAAGGGGTAGAGGATCAAGGAGAGGTAGGGGAAGAGGGCGGGGTACTGGCAAAATGGCGGATCCTAAAATGAGCTTCCGTGATTTTTGA

Protein Analysis

235

Amino Acids

25.63

Weight (kDa)

8.42

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 454
AciI CCGC 4 cut(s) 278, 454, 658, 677
AclWI GGATC 4 cut(s) 12, 640, 674, 687
AcsI RAATTY 2 cut(s) 56, 295
AfaI GTAC 2 cut(s) 590, 664
AfiI CCNNNNNNNGG 3 cut(s) 137, 191, 424
AflIII ACRYGT 2 cut(s) 287, 393
AgsI TTSAA 2 cut(s) 191, 615
AhlI ACTAGT 1 cut(s) 574
AjnI CCWGG 2 cut(s) 130, 136
AloI GAACNNNNNNTCC 2 cut(s) 333, 365
AluBI AGCT 4 cut(s) 112, 215, 241, 693
AluI AGCT 4 cut(s) 112, 215, 241, 693
Alw21I GWGCWC 2 cut(s) 370, 414
AlwI GGATC 4 cut(s) 12, 640, 674, 687
AoxI GGCC 1 cut(s) 133
ApeKI GCWGC 1 cut(s) 215
ApoI RAATTY 2 cut(s) 56, 295
ArsI GACNNNNNNTTYG 2 cut(s) 191, 223
AseI ATTAAT 1 cut(s) 116
AspS9I GGNCC 1 cut(s) 134
BamHI GGATCC 2 cut(s) 4, 679
BanII GRGCYC 1 cut(s) 430
Bbv12I GWGCWC 2 cut(s) 370, 414
BbvI GCAGC 1 cut(s) 202
BccI CCATC 1 cut(s) 515
BcgI CGANNNNNNTGC 2 cut(s) 369, 403
BciT130I CCWGG 2 cut(s) 132, 138
BcuI ACTAGT 1 cut(s) 574
BfaI CTAG 2 cut(s) 353, 575
BglI GCCNNNNNGGC 1 cut(s) 141
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
Bme1390I CCNGG 2 cut(s) 132, 138
BmgT120I GGNCC 1 cut(s) 134
BmiI GGNNCC 2 cut(s) 6, 681
BmrFI CCNGG 2 cut(s) 132, 138
BmsI GCATC 2 cut(s) 25, 289
BplI GAGNNNNNCTC 2 cut(s) 455, 487
BpmI CTGGAG 1 cut(s) 432
BsaJI CCNNGG 4 cut(s) 136, 310, 421, 513
BsaXI ACNNNNNCTCC 2 cut(s) 572, 602
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 191, 424
Bse1I ACTGG 3 cut(s) 21, 544, 670
BseBI CCWGG 2 cut(s) 132, 138
BseDI CCNNGG 4 cut(s) 136, 310, 421, 513
BseGI GGATG 2 cut(s) 40, 280
BseLI CCNNNNNNNGG 3 cut(s) 137, 191, 424
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 3 cut(s) 21, 544, 670
BseRI GAGGAG 1 cut(s) 404
BseXI GCAGC 1 cut(s) 202
BseYI CCCAGC 1 cut(s) 241
BshFI GGCC 1 cut(s) 135
BsiHKAI GWGCWC 2 cut(s) 370, 414
BslI CCNNNNNNNGG 3 cut(s) 137, 191, 424
BsnI GGCC 1 cut(s) 135
Bsp1286I GDGCHC 3 cut(s) 370, 414, 430
Bsp143I GATC 3 cut(s) 4, 632, 679
BspACI CCGC 4 cut(s) 278, 454, 658, 677
BspANI GGCC 1 cut(s) 135
BspCNI CTCAG 1 cut(s) 454
BspLI GGNNCC 2 cut(s) 6, 681
BspPI GGATC 4 cut(s) 12, 640, 674, 687
BsrBI CCGCTC 1 cut(s) 454
BsrI ACTGG 3 cut(s) 21, 544, 670
BssECI CCNNGG 4 cut(s) 136, 310, 421, 513
BssMI GATC 3 cut(s) 4, 632, 679
BssT1I CCWWGG 3 cut(s) 310, 421, 513
Bst2UI CCWGG 2 cut(s) 132, 138
Bst6I CTCTTC 1 cut(s) 646
BstAPI GCANNNNNTGC 2 cut(s) 264, 396
BstC8I GCNNGC 5 cut(s) 239, 256, 260, 278, 325
BstDEI CTNAG 1 cut(s) 462
BstENI CCTNNNNNAGG 1 cut(s) 135
BstF5I GGATG 2 cut(s) 40, 280
BstKTI GATC 3 cut(s) 7, 635, 682
BstMBI GATC 3 cut(s) 4, 632, 679
BstMWI GCNNNNNNNGC 5 cut(s) 141, 168, 264, 273, 396
BstNI CCWGG 2 cut(s) 132, 138
BstNSI RCATGY 4 cut(s) 93, 262, 291, 397
BstSCI CCNGG 2 cut(s) 130, 136
BstV1I GCAGC 1 cut(s) 202
BstX2I RGATCY 2 cut(s) 4, 679
BstXI CCANNNNNNTGG 1 cut(s) 539
BstYI RGATCY 2 cut(s) 4, 679
BsuRI GGCC 1 cut(s) 135
BtsCI GGATG 2 cut(s) 40, 280
Cac8I GCNNGC 5 cut(s) 239, 256, 260, 278, 325
Cfr13I GGNCC 1 cut(s) 134
Csp6I GTAC 2 cut(s) 589, 663
CviAII CATG 8 cut(s) 31, 65, 90, 127, 259, 264, 288, 394
CviJI RGCY 8 cut(s) 20, 112, 135, 215, 241, 254, 428, 693
CviKI_1 RGCY 8 cut(s) 20, 112, 135, 215, 241, 254, 428, 693
CviQI GTAC 2 cut(s) 589, 663
DdeI CTNAG 1 cut(s) 462
DpnI GATC 3 cut(s) 6, 634, 681
DpnII GATC 3 cut(s) 4, 632, 679
Eam1104I CTCTTC 1 cut(s) 646
EarI CTCTTC 1 cut(s) 646
EciI GGCGGA 1 cut(s) 692
Eco130I CCWWGG 3 cut(s) 310, 421, 513
Eco24I GRGCYC 1 cut(s) 430
Eco32I GATATC 1 cut(s) 306
EcoNI CCTNNNNNAGG 1 cut(s) 135
EcoRII CCWGG 2 cut(s) 130, 136
EcoRV GATATC 1 cut(s) 306
EcoT14I CCWWGG 3 cut(s) 310, 421, 513
EcoT38I GRGCYC 1 cut(s) 430
ErhI CCWWGG 3 cut(s) 310, 421, 513
FaeI CATG 8 cut(s) 34, 68, 93, 130, 262, 267, 291, 397
FatI CATG 8 cut(s) 30, 64, 89, 126, 258, 263, 287, 393
FauI CCCGC 2 cut(s) 285, 651
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 2 cut(s) 47, 267
FriOI GRGCYC 1 cut(s) 430
Fsp4HI GCNGC 1 cut(s) 216
FspBI CTAG 2 cut(s) 353, 575
GluI GCNGC 1 cut(s) 216
GsaI CCCAGC 1 cut(s) 245
GsuI CTGGAG 1 cut(s) 432
HaeIII GGCC 1 cut(s) 135
Hin1II CATG 8 cut(s) 34, 68, 93, 130, 262, 267, 291, 397
HinfI GANTC 2 cut(s) 315, 470
Hpy166II GTNNAC 1 cut(s) 490
Hpy188I TCNGA 1 cut(s) 608
Hpy188III TCNNGA 5 cut(s) 98, 353, 449, 461, 615
Hpy8I GTNNAC 1 cut(s) 490
HpyAV CCTTC 2 cut(s) 185, 615
HpyCH4V TGCA 3 cut(s) 218, 390, 399
HpyF10VI GCNNNNNNNGC 5 cut(s) 141, 168, 264, 273, 396
HpyF3I CTNAG 1 cut(s) 462
Hsp92II CATG 8 cut(s) 34, 68, 93, 130, 262, 267, 291, 397
Kzo9I GATC 3 cut(s) 4, 632, 679
LmnI GCTCC 4 cut(s) 128, 212, 373, 417
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 2 cut(s) 25, 289
MaeI CTAG 2 cut(s) 353, 575
MaeIII GTNAC 2 cut(s) 467, 561
MalI GATC 3 cut(s) 6, 634, 681
MbiI CCGCTC 1 cut(s) 454
MboI GATC 3 cut(s) 4, 632, 679
MboII GAAGA 2 cut(s) 530, 663
MflI RGATCY 2 cut(s) 4, 679
MhlI GDGCHC 3 cut(s) 370, 414, 430
MluCI AATT 3 cut(s) 56, 295, 400
MlyI GAGTC 2 cut(s) 324, 464
MnlI CCTC 8 cut(s) 105, 192, 425, 602, 611, 623, 635, 647
MseI TTAA 3 cut(s) 60, 116, 495
MslI CAYNNNNRTG 2 cut(s) 29, 485
MspR9I CCNGG 2 cut(s) 132, 138
MvaI CCWGG 2 cut(s) 132, 138
MwoI GCNNNNNNNGC 5 cut(s) 141, 168, 264, 273, 396
NdeII GATC 3 cut(s) 4, 632, 679
NlaIII CATG 8 cut(s) 34, 68, 93, 130, 262, 267, 291, 397
NlaIV GGNNCC 2 cut(s) 6, 681
NmuCI GTSAC 1 cut(s) 467
NspI RCATGY 4 cut(s) 93, 262, 291, 397
PaeI GCATGC 1 cut(s) 262
PciI ACATGT 2 cut(s) 287, 393
PkrI GCNGC 1 cut(s) 217
PleI GAGTC 2 cut(s) 323, 464
PpsI GAGTC 2 cut(s) 323, 464
PscI ACATGT 2 cut(s) 287, 393
PshBI ATTAAT 1 cut(s) 116
Psp6I CCWGG 2 cut(s) 130, 136
PspFI CCCAGC 1 cut(s) 241
PspGI CCWGG 2 cut(s) 130, 136
PspN4I GGNNCC 2 cut(s) 6, 681
PspPI GGNCC 1 cut(s) 134
PsuI RGATCY 2 cut(s) 4, 679
RsaI GTAC 2 cut(s) 590, 664
RsaNI GTAC 2 cut(s) 589, 663
RseI CAYNNNNRTG 2 cut(s) 29, 485
SaqAI TTAA 3 cut(s) 60, 116, 495
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 3 cut(s) 4, 632, 679
Sau96I GGNCC 1 cut(s) 134
SchI GAGTC 2 cut(s) 324, 464
ScrFI CCNGG 2 cut(s) 132, 138
SduI GDGCHC 3 cut(s) 370, 414, 430
SetI ASST 8 cut(s) 114, 142, 196, 217, 243, 613, 646, 695
SfaNI GCATC 2 cut(s) 25, 289
SmiMI CAYNNNNRTG 2 cut(s) 29, 485
SpeI ACTAGT 1 cut(s) 574
SphI GCATGC 1 cut(s) 262
Sse9I AATT 3 cut(s) 56, 295, 400
SsiI CCGC 4 cut(s) 278, 454, 658, 677
SspMI CTAG 2 cut(s) 353, 575
StyD4I CCNGG 2 cut(s) 130, 136
StyI CCWWGG 3 cut(s) 310, 421, 513
TaqI TCGA 3 cut(s) 198, 292, 473
TasI AATT 3 cut(s) 56, 295, 400
Tru1I TTAA 3 cut(s) 60, 116, 495
Tru9I TTAA 3 cut(s) 60, 116, 495
TseFI GTSAC 1 cut(s) 467
TseI GCWGC 1 cut(s) 215
Tsp45I GTSAC 1 cut(s) 467
TspDTI ATGAA 5 cut(s) 19, 219, 288, 557, 599
TspGWI ACGGA 2 cut(s) 606, 686
VspI ATTAAT 1 cut(s) 116
XagI CCTNNNNNAGG 1 cut(s) 135
XapI RAATTY 2 cut(s) 56, 295
XbaI TCTAGA 1 cut(s) 352
XceI RCATGY 4 cut(s) 93, 262, 291, 397
XspI CTAG 2 cut(s) 353, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.