Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G32000 AT2G32000 |
| fragaria_vesca | FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 |
| malus_domestica | MD04G1023600.v1.1 |
| prunus_persica | Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 |
| pyrus_communis | pycom04g02020 |
| rosa_chinensis | RchiOBHm_Chr4g0412031 |
| rosa_laevigata | RLG00000008327 |
| rosa_multiflora | Rmu_co8041726.1_g000001 Rmu_sc0000711.1_g000051 |
| rosa_roxburghii | Rroxscaffold_1G00042480 Rroxscaffold_5G00346360 Rroxscaffold_5G00356250 Rroxscaffold_5G00356340 |
| rosa_rugosa | Rorug04G0106700 Rorug04G0106800 Rorug04G0106900 |
| rosa_samantha | Rh4AG171100 Rh4BG168800 Rh4BG169700 Rh4CG181900 Rh4DG164200 Rh4DG165300 |
| rosa_wichuraiana | Rw0G007770 Rw0G008800 Rw4G014260 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 454 |
| AciI | CCGC | 4 cut(s) | 278, 454, 658, 677 |
| AclWI | GGATC | 4 cut(s) | 12, 640, 674, 687 |
| AcsI | RAATTY | 2 cut(s) | 56, 295 |
| AfaI | GTAC | 2 cut(s) | 590, 664 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 137, 191, 424 |
| AflIII | ACRYGT | 2 cut(s) | 287, 393 |
| AgsI | TTSAA | 2 cut(s) | 191, 615 |
| AhlI | ACTAGT | 1 cut(s) | 574 |
| AjnI | CCWGG | 2 cut(s) | 130, 136 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 333, 365 |
| AluBI | AGCT | 4 cut(s) | 112, 215, 241, 693 |
| AluI | AGCT | 4 cut(s) | 112, 215, 241, 693 |
| Alw21I | GWGCWC | 2 cut(s) | 370, 414 |
| AlwI | GGATC | 4 cut(s) | 12, 640, 674, 687 |
| AoxI | GGCC | 1 cut(s) | 133 |
| ApeKI | GCWGC | 1 cut(s) | 215 |
| ApoI | RAATTY | 2 cut(s) | 56, 295 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 191, 223 |
| AseI | ATTAAT | 1 cut(s) | 116 |
| AspS9I | GGNCC | 1 cut(s) | 134 |
| BamHI | GGATCC | 2 cut(s) | 4, 679 |
| BanII | GRGCYC | 1 cut(s) | 430 |
| Bbv12I | GWGCWC | 2 cut(s) | 370, 414 |
| BbvI | GCAGC | 1 cut(s) | 202 |
| BccI | CCATC | 1 cut(s) | 515 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 369, 403 |
| BciT130I | CCWGG | 2 cut(s) | 132, 138 |
| BcuI | ACTAGT | 1 cut(s) | 574 |
| BfaI | CTAG | 2 cut(s) | 353, 575 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 141 |
| BisI | GCNGC | 1 cut(s) | 216 |
| BlsI | GCNGC | 1 cut(s) | 217 |
| Bme1390I | CCNGG | 2 cut(s) | 132, 138 |
| BmgT120I | GGNCC | 1 cut(s) | 134 |
| BmiI | GGNNCC | 2 cut(s) | 6, 681 |
| BmrFI | CCNGG | 2 cut(s) | 132, 138 |
| BmsI | GCATC | 2 cut(s) | 25, 289 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 455, 487 |
| BpmI | CTGGAG | 1 cut(s) | 432 |
| BsaJI | CCNNGG | 4 cut(s) | 136, 310, 421, 513 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 572, 602 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 137, 191, 424 |
| Bse1I | ACTGG | 3 cut(s) | 21, 544, 670 |
| BseBI | CCWGG | 2 cut(s) | 132, 138 |
| BseDI | CCNNGG | 4 cut(s) | 136, 310, 421, 513 |
| BseGI | GGATG | 2 cut(s) | 40, 280 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 137, 191, 424 |
| BseMII | CTCAG | 1 cut(s) | 453 |
| BseNI | ACTGG | 3 cut(s) | 21, 544, 670 |
| BseRI | GAGGAG | 1 cut(s) | 404 |
| BseXI | GCAGC | 1 cut(s) | 202 |
| BseYI | CCCAGC | 1 cut(s) | 241 |
| BshFI | GGCC | 1 cut(s) | 135 |
| BsiHKAI | GWGCWC | 2 cut(s) | 370, 414 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 137, 191, 424 |
| BsnI | GGCC | 1 cut(s) | 135 |
| Bsp1286I | GDGCHC | 3 cut(s) | 370, 414, 430 |
| Bsp143I | GATC | 3 cut(s) | 4, 632, 679 |
| BspACI | CCGC | 4 cut(s) | 278, 454, 658, 677 |
| BspANI | GGCC | 1 cut(s) | 135 |
| BspCNI | CTCAG | 1 cut(s) | 454 |
| BspLI | GGNNCC | 2 cut(s) | 6, 681 |
| BspPI | GGATC | 4 cut(s) | 12, 640, 674, 687 |
| BsrBI | CCGCTC | 1 cut(s) | 454 |
| BsrI | ACTGG | 3 cut(s) | 21, 544, 670 |
| BssECI | CCNNGG | 4 cut(s) | 136, 310, 421, 513 |
| BssMI | GATC | 3 cut(s) | 4, 632, 679 |
| BssT1I | CCWWGG | 3 cut(s) | 310, 421, 513 |
| Bst2UI | CCWGG | 2 cut(s) | 132, 138 |
| Bst6I | CTCTTC | 1 cut(s) | 646 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 264, 396 |
| BstC8I | GCNNGC | 5 cut(s) | 239, 256, 260, 278, 325 |
| BstDEI | CTNAG | 1 cut(s) | 462 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 135 |
| BstF5I | GGATG | 2 cut(s) | 40, 280 |
| BstKTI | GATC | 3 cut(s) | 7, 635, 682 |
| BstMBI | GATC | 3 cut(s) | 4, 632, 679 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 141, 168, 264, 273, 396 |
| BstNI | CCWGG | 2 cut(s) | 132, 138 |
| BstNSI | RCATGY | 4 cut(s) | 93, 262, 291, 397 |
| BstSCI | CCNGG | 2 cut(s) | 130, 136 |
| BstV1I | GCAGC | 1 cut(s) | 202 |
| BstX2I | RGATCY | 2 cut(s) | 4, 679 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 539 |
| BstYI | RGATCY | 2 cut(s) | 4, 679 |
| BsuRI | GGCC | 1 cut(s) | 135 |
| BtsCI | GGATG | 2 cut(s) | 40, 280 |
| Cac8I | GCNNGC | 5 cut(s) | 239, 256, 260, 278, 325 |
| Cfr13I | GGNCC | 1 cut(s) | 134 |
| Csp6I | GTAC | 2 cut(s) | 589, 663 |
| CviAII | CATG | 8 cut(s) | 31, 65, 90, 127, 259, 264, 288, 394 |
| CviJI | RGCY | 8 cut(s) | 20, 112, 135, 215, 241, 254, 428, 693 |
| CviKI_1 | RGCY | 8 cut(s) | 20, 112, 135, 215, 241, 254, 428, 693 |
| CviQI | GTAC | 2 cut(s) | 589, 663 |
| DdeI | CTNAG | 1 cut(s) | 462 |
| DpnI | GATC | 3 cut(s) | 6, 634, 681 |
| DpnII | GATC | 3 cut(s) | 4, 632, 679 |
| Eam1104I | CTCTTC | 1 cut(s) | 646 |
| EarI | CTCTTC | 1 cut(s) | 646 |
| EciI | GGCGGA | 1 cut(s) | 692 |
| Eco130I | CCWWGG | 3 cut(s) | 310, 421, 513 |
| Eco24I | GRGCYC | 1 cut(s) | 430 |
| Eco32I | GATATC | 1 cut(s) | 306 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 135 |
| EcoRII | CCWGG | 2 cut(s) | 130, 136 |
| EcoRV | GATATC | 1 cut(s) | 306 |
| EcoT14I | CCWWGG | 3 cut(s) | 310, 421, 513 |
| EcoT38I | GRGCYC | 1 cut(s) | 430 |
| ErhI | CCWWGG | 3 cut(s) | 310, 421, 513 |
| FaeI | CATG | 8 cut(s) | 34, 68, 93, 130, 262, 267, 291, 397 |
| FatI | CATG | 8 cut(s) | 30, 64, 89, 126, 258, 263, 287, 393 |
| FauI | CCCGC | 2 cut(s) | 285, 651 |
| Fnu4HI | GCNGC | 1 cut(s) | 216 |
| FokI | GGATG | 2 cut(s) | 47, 267 |
| FriOI | GRGCYC | 1 cut(s) | 430 |
| Fsp4HI | GCNGC | 1 cut(s) | 216 |
| FspBI | CTAG | 2 cut(s) | 353, 575 |
| GluI | GCNGC | 1 cut(s) | 216 |
| GsaI | CCCAGC | 1 cut(s) | 245 |
| GsuI | CTGGAG | 1 cut(s) | 432 |
| HaeIII | GGCC | 1 cut(s) | 135 |
| Hin1II | CATG | 8 cut(s) | 34, 68, 93, 130, 262, 267, 291, 397 |
| HinfI | GANTC | 2 cut(s) | 315, 470 |
| Hpy166II | GTNNAC | 1 cut(s) | 490 |
| Hpy188I | TCNGA | 1 cut(s) | 608 |
| Hpy188III | TCNNGA | 5 cut(s) | 98, 353, 449, 461, 615 |
| Hpy8I | GTNNAC | 1 cut(s) | 490 |
| HpyAV | CCTTC | 2 cut(s) | 185, 615 |
| HpyCH4V | TGCA | 3 cut(s) | 218, 390, 399 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 141, 168, 264, 273, 396 |
| HpyF3I | CTNAG | 1 cut(s) | 462 |
| Hsp92II | CATG | 8 cut(s) | 34, 68, 93, 130, 262, 267, 291, 397 |
| Kzo9I | GATC | 3 cut(s) | 4, 632, 679 |
| LmnI | GCTCC | 4 cut(s) | 128, 212, 373, 417 |
| Lsp1109I | GCAGC | 1 cut(s) | 202 |
| LweI | GCATC | 2 cut(s) | 25, 289 |
| MaeI | CTAG | 2 cut(s) | 353, 575 |
| MaeIII | GTNAC | 2 cut(s) | 467, 561 |
| MalI | GATC | 3 cut(s) | 6, 634, 681 |
| MbiI | CCGCTC | 1 cut(s) | 454 |
| MboI | GATC | 3 cut(s) | 4, 632, 679 |
| MboII | GAAGA | 2 cut(s) | 530, 663 |
| MflI | RGATCY | 2 cut(s) | 4, 679 |
| MhlI | GDGCHC | 3 cut(s) | 370, 414, 430 |
| MluCI | AATT | 3 cut(s) | 56, 295, 400 |
| MlyI | GAGTC | 2 cut(s) | 324, 464 |
| MnlI | CCTC | 8 cut(s) | 105, 192, 425, 602, 611, 623, 635, 647 |
| MseI | TTAA | 3 cut(s) | 60, 116, 495 |
| MslI | CAYNNNNRTG | 2 cut(s) | 29, 485 |
| MspR9I | CCNGG | 2 cut(s) | 132, 138 |
| MvaI | CCWGG | 2 cut(s) | 132, 138 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 141, 168, 264, 273, 396 |
| NdeII | GATC | 3 cut(s) | 4, 632, 679 |
| NlaIII | CATG | 8 cut(s) | 34, 68, 93, 130, 262, 267, 291, 397 |
| NlaIV | GGNNCC | 2 cut(s) | 6, 681 |
| NmuCI | GTSAC | 1 cut(s) | 467 |
| NspI | RCATGY | 4 cut(s) | 93, 262, 291, 397 |
| PaeI | GCATGC | 1 cut(s) | 262 |
| PciI | ACATGT | 2 cut(s) | 287, 393 |
| PkrI | GCNGC | 1 cut(s) | 217 |
| PleI | GAGTC | 2 cut(s) | 323, 464 |
| PpsI | GAGTC | 2 cut(s) | 323, 464 |
| PscI | ACATGT | 2 cut(s) | 287, 393 |
| PshBI | ATTAAT | 1 cut(s) | 116 |
| Psp6I | CCWGG | 2 cut(s) | 130, 136 |
| PspFI | CCCAGC | 1 cut(s) | 241 |
| PspGI | CCWGG | 2 cut(s) | 130, 136 |
| PspN4I | GGNNCC | 2 cut(s) | 6, 681 |
| PspPI | GGNCC | 1 cut(s) | 134 |
| PsuI | RGATCY | 2 cut(s) | 4, 679 |
| RsaI | GTAC | 2 cut(s) | 590, 664 |
| RsaNI | GTAC | 2 cut(s) | 589, 663 |
| RseI | CAYNNNNRTG | 2 cut(s) | 29, 485 |
| SaqAI | TTAA | 3 cut(s) | 60, 116, 495 |
| SatI | GCNGC | 1 cut(s) | 216 |
| Sau3AI | GATC | 3 cut(s) | 4, 632, 679 |
| Sau96I | GGNCC | 1 cut(s) | 134 |
| SchI | GAGTC | 2 cut(s) | 324, 464 |
| ScrFI | CCNGG | 2 cut(s) | 132, 138 |
| SduI | GDGCHC | 3 cut(s) | 370, 414, 430 |
| SetI | ASST | 8 cut(s) | 114, 142, 196, 217, 243, 613, 646, 695 |
| SfaNI | GCATC | 2 cut(s) | 25, 289 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 29, 485 |
| SpeI | ACTAGT | 1 cut(s) | 574 |
| SphI | GCATGC | 1 cut(s) | 262 |
| Sse9I | AATT | 3 cut(s) | 56, 295, 400 |
| SsiI | CCGC | 4 cut(s) | 278, 454, 658, 677 |
| SspMI | CTAG | 2 cut(s) | 353, 575 |
| StyD4I | CCNGG | 2 cut(s) | 130, 136 |
| StyI | CCWWGG | 3 cut(s) | 310, 421, 513 |
| TaqI | TCGA | 3 cut(s) | 198, 292, 473 |
| TasI | AATT | 3 cut(s) | 56, 295, 400 |
| Tru1I | TTAA | 3 cut(s) | 60, 116, 495 |
| Tru9I | TTAA | 3 cut(s) | 60, 116, 495 |
| TseFI | GTSAC | 1 cut(s) | 467 |
| TseI | GCWGC | 1 cut(s) | 215 |
| Tsp45I | GTSAC | 1 cut(s) | 467 |
| TspDTI | ATGAA | 5 cut(s) | 19, 219, 288, 557, 599 |
| TspGWI | ACGGA | 2 cut(s) | 606, 686 |
| VspI | ATTAAT | 1 cut(s) | 116 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 135 |
| XapI | RAATTY | 2 cut(s) | 56, 295 |
| XbaI | TCTAGA | 1 cut(s) | 352 |
| XceI | RCATGY | 4 cut(s) | 93, 262, 291, 397 |
| XspI | CTAG | 2 cut(s) | 353, 575 |