Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G32000 AT2G32000 |
| fragaria_vesca | FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 |
| malus_domestica | MD04G1023600.v1.1 |
| prunus_persica | Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 |
| pyrus_communis | pycom04g02020 |
| rosa_chinensis | RchiOBHm_Chr4g0412031 |
| rosa_laevigata | RLG00000008327 |
| rosa_multiflora | Rmu_co8041726.1_g000001 Rmu_sc0000711.1_g000051 |
| rosa_roxburghii | Rroxscaffold_1G00042480 Rroxscaffold_5G00346360 Rroxscaffold_5G00356250 Rroxscaffold_5G00356340 |
| rosa_rugosa | Rorug04G0106700 Rorug04G0106800 Rorug04G0106900 |
| rosa_samantha | Rh4AG171100 Rh4BG168800 Rh4BG169700 Rh4CG181900 Rh4DG164200 Rh4DG165300 |
| rosa_wichuraiana | Rw0G007770 Rw0G008800 Rw4G014260 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 780 |
| Acc36I | ACCTGC | 1 cut(s) | 1021 |
| Acc65I | GGTACC | 1 cut(s) | 1415 |
| AccB1I | GGYRCC | 1 cut(s) | 1415 |
| AccBSI | CCGCTC | 1 cut(s) | 1807 |
| AccI | GTMKAC | 3 cut(s) | 86, 1330, 1645 |
| AccII | CGCG | 1 cut(s) | 75 |
| AciI | CCGC | 5 cut(s) | 73, 75, 1129, 1807, 2011 |
| AclI | AACGTT | 1 cut(s) | 63 |
| AclWI | GGATC | 6 cut(s) | 221, 243, 1097, 1993, 2027, 2040 |
| AcoI | YGGCCR | 1 cut(s) | 394 |
| AcsI | RAATTY | 2 cut(s) | 116, 698 |
| AcuI | CTGAAG | 1 cut(s) | 728 |
| AcyI | GRCGYC | 1 cut(s) | 777 |
| AfaI | GTAC | 8 cut(s) | 111, 147, 660, 711, 1035, 1417, 1430, 2017 |
| AfeI | AGCGCT | 1 cut(s) | 665 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 647, 1412, 1544, 1777 |
| AflIII | ACRYGT | 2 cut(s) | 1640, 1746 |
| AhlI | ACTAGT | 2 cut(s) | 572, 1927 |
| AjnI | CCWGG | 1 cut(s) | 1483 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1686, 1718 |
| Alw21I | GWGCWC | 3 cut(s) | 433, 1723, 1767 |
| Alw26I | GTCTC | 1 cut(s) | 1373 |
| Alw44I | GTGCAC | 1 cut(s) | 429 |
| AlwI | GGATC | 6 cut(s) | 221, 243, 1097, 1993, 2027, 2040 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 224 |
| Ama87I | CYCGRG | 1 cut(s) | 312 |
| Aor51HI | AGCGCT | 1 cut(s) | 665 |
| AoxI | GGCC | 4 cut(s) | 394, 468, 626, 1486 |
| ApaLI | GTGCAC | 1 cut(s) | 429 |
| ApeKI | GCWGC | 3 cut(s) | 1144, 1195, 1568 |
| ApoI | RAATTY | 2 cut(s) | 116, 698 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1544, 1576 |
| AseI | ATTAAT | 1 cut(s) | 1469 |
| Asp718I | GGTACC | 1 cut(s) | 1415 |
| AspLEI | GCGC | 2 cut(s) | 666, 1020 |
| AspS9I | GGNCC | 2 cut(s) | 626, 1487 |
| AsuHPI | GGTGA | 1 cut(s) | 906 |
| AsuNHI | GCTAGC | 1 cut(s) | 98 |
| AvaI | CYCGRG | 1 cut(s) | 312 |
| AxyI | CCTNAGG | 1 cut(s) | 1066 |
| BaeGI | GKGCMC | 2 cut(s) | 433, 1448 |
| BalI | TGGCCA | 1 cut(s) | 396 |
| BamHI | GGATCC | 1 cut(s) | 2032 |
| BanI | GGYRCC | 1 cut(s) | 1415 |
| BanII | GRGCYC | 1 cut(s) | 1783 |
| Bbv12I | GWGCWC | 3 cut(s) | 433, 1723, 1767 |
| BbvI | GCAGC | 3 cut(s) | 1156, 1207, 1555 |
| BccI | CCATC | 3 cut(s) | 812, 1487, 1868 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1722, 1756 |
| BciT130I | CCWGG | 1 cut(s) | 1485 |
| BcoDI | GTCTC | 1 cut(s) | 1373 |
| BcuI | ACTAGT | 2 cut(s) | 572, 1927 |
| BfaI | CTAG | 5 cut(s) | 99, 573, 749, 1706, 1928 |
| BfmI | CTRYAG | 1 cut(s) | 513 |
| BfoI | RGCGCY | 1 cut(s) | 667 |
| BfuAI | ACCTGC | 1 cut(s) | 1021 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1494 |
| BglII | AGATCT | 1 cut(s) | 1108 |
| BisI | GCNGC | 3 cut(s) | 1145, 1196, 1569 |
| BlsI | GCNGC | 3 cut(s) | 1146, 1197, 1570 |
| Bme1390I | CCNGG | 1 cut(s) | 1485 |
| BmeT110I | CYCGRG | 1 cut(s) | 312 |
| BmgT120I | GGNCC | 2 cut(s) | 626, 1487 |
| BmiI | GGNNCC | 3 cut(s) | 1056, 1417, 2034 |
| BmrFI | CCNGG | 1 cut(s) | 1485 |
| BmrI | ACTGGG | 2 cut(s) | 763, 2028 |
| BmsI | GCATC | 5 cut(s) | 508, 595, 970, 992, 1249 |
| BmtI | GCTAGC | 1 cut(s) | 102 |
| BmuI | ACTGGG | 2 cut(s) | 763, 2028 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1808, 1840 |
| BpmI | CTGGAG | 3 cut(s) | 234, 903, 1785 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1164 |
| BsaAI | YACGTR | 1 cut(s) | 1443 |
| BsaBI | GATNNNNATC | 2 cut(s) | 348, 1107 |
| BsaHI | GRCGYC | 1 cut(s) | 777 |
| BsaJI | CCNNGG | 6 cut(s) | 73, 471, 646, 1663, 1774, 1866 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 53, 83, 901, 931, 1925, 1955 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 647, 1412, 1544, 1777 |
| Bse1I | ACTGG | 5 cut(s) | 218, 251, 758, 1897, 2023 |
| Bse21I | CCTNAGG | 1 cut(s) | 1066 |
| Bse8I | GATNNNNATC | 2 cut(s) | 348, 1107 |
| BseBI | CCWGG | 1 cut(s) | 1485 |
| BseDI | CCNNGG | 6 cut(s) | 73, 471, 646, 1663, 1774, 1866 |
| BseGI | GGATG | 3 cut(s) | 985, 1264, 1633 |
| BseJI | GATNNNNATC | 2 cut(s) | 348, 1107 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 647, 1412, 1544, 1777 |
| BseMII | CTCAG | 4 cut(s) | 1311, 1324, 1421, 1806 |
| BseNI | ACTGG | 5 cut(s) | 218, 251, 758, 1897, 2023 |
| BseRI | GAGGAG | 1 cut(s) | 1757 |
| BseSI | GKGCMC | 2 cut(s) | 433, 1448 |
| BseXI | GCAGC | 3 cut(s) | 1156, 1207, 1555 |
| BseYI | CCCAGC | 2 cut(s) | 38, 1594 |
| BsgI | GTGCAG | 2 cut(s) | 1049, 1214 |
| Bsh1236I | CGCG | 1 cut(s) | 75 |
| BshFI | GGCC | 4 cut(s) | 396, 470, 628, 1488 |
| BshNI | GGYRCC | 1 cut(s) | 1415 |
| BsiHKAI | GWGCWC | 3 cut(s) | 433, 1723, 1767 |
| BsiHKCI | CYCGRG | 1 cut(s) | 312 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 647, 1412, 1544, 1777 |
| BsmAI | GTCTC | 1 cut(s) | 1373 |
| BsmBI | CGTCTC | 1 cut(s) | 1373 |
| BsnI | GGCC | 4 cut(s) | 396, 470, 628, 1488 |
| BsoBI | CYCGRG | 1 cut(s) | 312 |
| Bsp1286I | GDGCHC | 5 cut(s) | 433, 1448, 1723, 1767, 1783 |
| Bsp1407I | TGTACA | 3 cut(s) | 145, 658, 1428 |
| Bsp143I | GATC | 8 cut(s) | 226, 235, 349, 1102, 1108, 1177, 1985, 2032 |
| Bsp19I | CCATGG | 1 cut(s) | 471 |
| BspACI | CCGC | 5 cut(s) | 73, 75, 1129, 1807, 2011 |
| BspANI | GGCC | 4 cut(s) | 396, 470, 628, 1488 |
| BspCNI | CTCAG | 4 cut(s) | 1310, 1323, 1420, 1807 |
| BspFNI | CGCG | 1 cut(s) | 75 |
| BspLI | GGNNCC | 3 cut(s) | 1056, 1417, 2034 |
| BspMI | ACCTGC | 1 cut(s) | 1021 |
| BspOI | GCTAGC | 1 cut(s) | 102 |
| BspPI | GGATC | 6 cut(s) | 221, 243, 1097, 1993, 2027, 2040 |
| BspT107I | GGYRCC | 1 cut(s) | 1415 |
| BsrBI | CCGCTC | 1 cut(s) | 1807 |
| BsrGI | TGTACA | 3 cut(s) | 145, 658, 1428 |
| BsrI | ACTGG | 5 cut(s) | 218, 251, 758, 1897, 2023 |
| BssECI | CCNNGG | 6 cut(s) | 73, 471, 646, 1663, 1774, 1866 |
| BssMI | GATC | 8 cut(s) | 226, 235, 349, 1102, 1108, 1177, 1985, 2032 |
| BssNI | GRCGYC | 1 cut(s) | 777 |
| BssT1I | CCWWGG | 5 cut(s) | 471, 646, 1663, 1774, 1866 |
| Bst2UI | CCWGG | 1 cut(s) | 1485 |
| Bst4CI | ACNGT | 7 cut(s) | 150, 220, 889, 1212, 1229, 1335, 1376 |
| Bst6I | CTCTTC | 2 cut(s) | 1383, 1999 |
| BstACI | GRCGYC | 1 cut(s) | 777 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1617, 1749 |
| BstAUI | TGTACA | 3 cut(s) | 145, 658, 1428 |
| BstBAI | YACGTR | 1 cut(s) | 1443 |
| BstC8I | GCNNGC | 7 cut(s) | 100, 293, 1042, 1592, 1609, 1613, 1678 |
| BstDEI | CTNAG | 7 cut(s) | 929, 1066, 1164, 1297, 1310, 1407, 1815 |
| BstDSI | CCRYGG | 2 cut(s) | 73, 471 |
| BstF5I | GGATG | 3 cut(s) | 985, 1264, 1633 |
| BstFNI | CGCG | 1 cut(s) | 75 |
| BstH2I | RGCGCY | 1 cut(s) | 667 |
| BstHHI | GCGC | 2 cut(s) | 666, 1020 |
| BstKTI | GATC | 8 cut(s) | 229, 238, 352, 1105, 1111, 1180, 1988, 2035 |
| BstMAI | GTCTC | 1 cut(s) | 1373 |
| BstMBI | GATC | 8 cut(s) | 226, 235, 349, 1102, 1108, 1177, 1985, 2032 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 518, 670, 949, 1494, 1521, 1617, 1626, 1749 |
| BstNI | CCWGG | 1 cut(s) | 1485 |
| BstNSI | RCATGY | 6 cut(s) | 179, 1202, 1386, 1615, 1644, 1750 |
| BstSCI | CCNGG | 1 cut(s) | 1483 |
| BstSFI | CTRYAG | 1 cut(s) | 513 |
| BstSLI | GKGCMC | 2 cut(s) | 433, 1448 |
| BstUI | CGCG | 1 cut(s) | 75 |
| BstV1I | GCAGC | 3 cut(s) | 1156, 1207, 1555 |
| BstX2I | RGATCY | 3 cut(s) | 235, 1108, 2032 |
| BstYI | RGATCY | 3 cut(s) | 235, 1108, 2032 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1066 |
| BsuRI | GGCC | 4 cut(s) | 396, 470, 628, 1488 |
| BtgI | CCRYGG | 2 cut(s) | 73, 471 |
| BtsCI | GGATG | 3 cut(s) | 985, 1264, 1633 |
| BtsIMutI | CAGTG | 2 cut(s) | 222, 894 |
| BveI | ACCTGC | 1 cut(s) | 1021 |
| Cac8I | GCNNGC | 7 cut(s) | 100, 293, 1042, 1592, 1609, 1613, 1678 |
| CaiI | CAGNNNCTG | 1 cut(s) | 224 |
| CfoI | GCGC | 2 cut(s) | 666, 1020 |
| Cfr13I | GGNCC | 2 cut(s) | 626, 1487 |
| Cfr42I | CCGCGG | 1 cut(s) | 76 |
| Csp6I | GTAC | 8 cut(s) | 110, 146, 659, 710, 1034, 1416, 1429, 2016 |
| CviQI | GTAC | 8 cut(s) | 110, 146, 659, 710, 1034, 1416, 1429, 2016 |
| DdeI | CTNAG | 7 cut(s) | 929, 1066, 1164, 1297, 1310, 1407, 1815 |
| DpnI | GATC | 8 cut(s) | 228, 237, 351, 1104, 1110, 1179, 1987, 2034 |
| DpnII | GATC | 8 cut(s) | 226, 235, 349, 1102, 1108, 1177, 1985, 2032 |
| DraI | TTTAAA | 2 cut(s) | 154, 688 |
| EaeI | YGGCCR | 1 cut(s) | 394 |
| Eam1104I | CTCTTC | 2 cut(s) | 1383, 1999 |
| EarI | CTCTTC | 2 cut(s) | 1383, 1999 |
| Eco130I | CCWWGG | 5 cut(s) | 471, 646, 1663, 1774, 1866 |
| Eco24I | GRGCYC | 1 cut(s) | 1783 |
| Eco32I | GATATC | 2 cut(s) | 1126, 1659 |
| Eco47III | AGCGCT | 1 cut(s) | 665 |
| Eco57I | CTGAAG | 1 cut(s) | 728 |
| Eco81I | CCTNAGG | 1 cut(s) | 1066 |
| Eco88I | CYCGRG | 1 cut(s) | 312 |
| EcoRII | CCWGG | 1 cut(s) | 1483 |
| EcoRV | GATATC | 2 cut(s) | 1126, 1659 |
| EcoT14I | CCWWGG | 5 cut(s) | 471, 646, 1663, 1774, 1866 |
| EcoT22I | ATGCAT | 2 cut(s) | 985, 1264 |
| EcoT38I | GRGCYC | 1 cut(s) | 1783 |
| ErhI | CCWWGG | 5 cut(s) | 471, 646, 1663, 1774, 1866 |
| Esp3I | CGTCTC | 1 cut(s) | 1373 |
| FauI | CCCGC | 2 cut(s) | 80, 2004 |
| FauNDI | CATATG | 1 cut(s) | 1010 |
| FblI | GTMKAC | 3 cut(s) | 86, 1330, 1645 |
| Fnu4HI | GCNGC | 3 cut(s) | 1145, 1196, 1569 |
| FokI | GGATG | 3 cut(s) | 992, 1271, 1620 |
| FriOI | GRGCYC | 1 cut(s) | 1783 |
| Fsp4HI | GCNGC | 3 cut(s) | 1145, 1196, 1569 |
| FspBI | CTAG | 5 cut(s) | 99, 573, 749, 1706, 1928 |
| GlaI | GCGC | 2 cut(s) | 665, 1019 |
| GluI | GCNGC | 3 cut(s) | 1145, 1196, 1569 |
| GsaI | CCCAGC | 2 cut(s) | 42, 1598 |
| GsuI | CTGGAG | 3 cut(s) | 234, 903, 1785 |
| HaeII | RGCGCY | 1 cut(s) | 667 |
| HaeIII | GGCC | 4 cut(s) | 396, 470, 628, 1488 |
| HhaI | GCGC | 2 cut(s) | 666, 1020 |
| Hin1I | GRCGYC | 1 cut(s) | 777 |
| Hin6I | GCGC | 2 cut(s) | 664, 1018 |
| HinP1I | GCGC | 2 cut(s) | 664, 1018 |
| HincII | GTYRAC | 1 cut(s) | 1646 |
| HindII | GTYRAC | 1 cut(s) | 1646 |
| HinfI | GANTC | 5 cut(s) | 138, 892, 969, 1295, 1823 |
| HphI | GGTGA | 1 cut(s) | 906 |
| Hpy166II | GTNNAC | 6 cut(s) | 87, 431, 659, 1331, 1646, 1843 |
| Hpy188I | TCNGA | 5 cut(s) | 548, 847, 1217, 1410, 1961 |
| Hpy8I | GTNNAC | 6 cut(s) | 87, 431, 659, 1331, 1646, 1843 |
| Hpy99I | CGWCG | 1 cut(s) | 782 |
| HpyAV | CCTTC | 7 cut(s) | 116, 350, 460, 882, 1371, 1538, 1968 |
| HpyCH4III | ACNGT | 7 cut(s) | 150, 220, 889, 1212, 1229, 1335, 1376 |
| HpyCH4IV | ACGT | 4 cut(s) | 63, 108, 777, 1442 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 518, 670, 949, 1494, 1521, 1617, 1626, 1749 |
| HpyF3I | CTNAG | 7 cut(s) | 929, 1066, 1164, 1297, 1310, 1407, 1815 |
| HpySE526I | ACGT | 4 cut(s) | 63, 108, 777, 1442 |
| Hsp92I | GRCGYC | 1 cut(s) | 777 |
| HspAI | GCGC | 2 cut(s) | 664, 1018 |
| KpnI | GGTACC | 1 cut(s) | 1419 |
| KspI | CCGCGG | 1 cut(s) | 76 |
| Kzo9I | GATC | 8 cut(s) | 226, 235, 349, 1102, 1108, 1177, 1985, 2032 |
| LmnI | GCTCC | 6 cut(s) | 253, 757, 1481, 1565, 1726, 1770 |
| Lsp1109I | GCAGC | 3 cut(s) | 1156, 1207, 1555 |
| LweI | GCATC | 5 cut(s) | 508, 595, 970, 992, 1249 |
| MaeI | CTAG | 5 cut(s) | 99, 573, 749, 1706, 1928 |
| MaeII | ACGT | 4 cut(s) | 63, 108, 777, 1442 |
| MaeIII | GTNAC | 8 cut(s) | 157, 220, 323, 445, 761, 832, 1820, 1914 |
| MalI | GATC | 8 cut(s) | 228, 237, 351, 1104, 1110, 1179, 1987, 2034 |
| MbiI | CCGCTC | 1 cut(s) | 1807 |
| MboI | GATC | 8 cut(s) | 226, 235, 349, 1102, 1108, 1177, 1985, 2032 |
| MboII | GAAGA | 8 cut(s) | 432, 827, 887, 973, 1394, 1400, 1883, 2016 |
| MflI | RGATCY | 3 cut(s) | 235, 1108, 2032 |
| MhlI | GDGCHC | 5 cut(s) | 433, 1448, 1723, 1767, 1783 |
| MlsI | TGGCCA | 1 cut(s) | 396 |
| MluCI | AATT | 8 cut(s) | 116, 698, 767, 822, 945, 1021, 1277, 1753 |
| MluNI | TGGCCA | 1 cut(s) | 396 |
| MlyI | GAGTC | 2 cut(s) | 1289, 1817 |
| MmeI | TCCRAC | 4 cut(s) | 33, 526, 1082, 1240 |
| Mox20I | TGGCCA | 1 cut(s) | 396 |
| Mph1103I | ATGCAT | 2 cut(s) | 985, 1264 |
| MscI | TGGCCA | 1 cut(s) | 396 |
| MseI | TTAA | 8 cut(s) | 153, 299, 687, 882, 1002, 1422, 1469, 1848 |
| MslI | CAYNNNNRTG | 3 cut(s) | 402, 1185, 1838 |
| Msp20I | TGGCCA | 1 cut(s) | 396 |
| MspA1I | CMGCKG | 1 cut(s) | 75 |
| MspR9I | CCNGG | 1 cut(s) | 1485 |
| MvaI | CCWGG | 1 cut(s) | 1485 |
| MvnI | CGCG | 1 cut(s) | 75 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 518, 670, 949, 1494, 1521, 1617, 1626, 1749 |
| NcoI | CCATGG | 1 cut(s) | 471 |
| NdeI | CATATG | 1 cut(s) | 1010 |
| NdeII | GATC | 8 cut(s) | 226, 235, 349, 1102, 1108, 1177, 1985, 2032 |
| NheI | GCTAGC | 1 cut(s) | 98 |
| NlaIV | GGNNCC | 3 cut(s) | 1056, 1417, 2034 |
| NmeAIII | GCCGAG | 1 cut(s) | 55 |
| NmuCI | GTSAC | 4 cut(s) | 157, 220, 832, 1820 |
| NsiI | ATGCAT | 2 cut(s) | 985, 1264 |
| NspI | RCATGY | 6 cut(s) | 179, 1202, 1386, 1615, 1644, 1750 |
| PaeI | GCATGC | 1 cut(s) | 1615 |
| PciI | ACATGT | 2 cut(s) | 1640, 1746 |
| PfeI | GAWTC | 3 cut(s) | 138, 892, 969 |
| PkrI | GCNGC | 3 cut(s) | 1146, 1197, 1570 |
| PleI | GAGTC | 2 cut(s) | 1289, 1817 |
| PpsI | GAGTC | 2 cut(s) | 1289, 1817 |
| Ppu21I | YACGTR | 1 cut(s) | 1443 |
| PscI | ACATGT | 2 cut(s) | 1640, 1746 |
| PshBI | ATTAAT | 1 cut(s) | 1469 |
| Psp1406I | AACGTT | 1 cut(s) | 63 |
| Psp6I | CCWGG | 1 cut(s) | 1483 |
| PspFI | CCCAGC | 2 cut(s) | 38, 1594 |
| PspGI | CCWGG | 1 cut(s) | 1483 |
| PspN4I | GGNNCC | 3 cut(s) | 1056, 1417, 2034 |
| PspPI | GGNCC | 2 cut(s) | 626, 1487 |
| PstNI | CAGNNNCTG | 1 cut(s) | 224 |
| PsuI | RGATCY | 3 cut(s) | 235, 1108, 2032 |
| RsaI | GTAC | 8 cut(s) | 111, 147, 660, 711, 1035, 1417, 1430, 2017 |
| RsaNI | GTAC | 8 cut(s) | 110, 146, 659, 710, 1034, 1416, 1429, 2016 |
| RseI | CAYNNNNRTG | 3 cut(s) | 402, 1185, 1838 |
| SacII | CCGCGG | 1 cut(s) | 76 |
| SalI | GTCGAC | 1 cut(s) | 1644 |
| SaqAI | TTAA | 8 cut(s) | 153, 299, 687, 882, 1002, 1422, 1469, 1848 |
| SatI | GCNGC | 3 cut(s) | 1145, 1196, 1569 |
| Sau3AI | GATC | 8 cut(s) | 226, 235, 349, 1102, 1108, 1177, 1985, 2032 |
| Sau96I | GGNCC | 2 cut(s) | 626, 1487 |
| SchI | GAGTC | 2 cut(s) | 1289, 1817 |
| ScrFI | CCNGG | 1 cut(s) | 1485 |
| SduI | GDGCHC | 5 cut(s) | 433, 1448, 1723, 1767, 1783 |
| SfaNI | GCATC | 5 cut(s) | 508, 595, 970, 992, 1249 |
| SfcI | CTRYAG | 1 cut(s) | 513 |
| Sfr303I | CCGCGG | 1 cut(s) | 76 |
| SgrBI | CCGCGG | 1 cut(s) | 76 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 402, 1185, 1838 |
| SpeI | ACTAGT | 2 cut(s) | 572, 1927 |
| SphI | GCATGC | 1 cut(s) | 1615 |
| Sse9I | AATT | 8 cut(s) | 116, 698, 767, 822, 945, 1021, 1277, 1753 |
| SsiI | CCGC | 5 cut(s) | 73, 75, 1129, 1807, 2011 |
| SspMI | CTAG | 5 cut(s) | 99, 573, 749, 1706, 1928 |
| StyD4I | CCNGG | 1 cut(s) | 1483 |
| StyI | CCWWGG | 5 cut(s) | 471, 646, 1663, 1774, 1866 |
| TaaI | ACNGT | 7 cut(s) | 150, 220, 889, 1212, 1229, 1335, 1376 |
| TaiI | ACGT | 4 cut(s) | 66, 111, 780, 1445 |
| TaqI | TCGA | 7 cut(s) | 523, 603, 780, 1551, 1645, 1826, 1981 |
| TasI | AATT | 8 cut(s) | 116, 698, 767, 822, 945, 1021, 1277, 1753 |
| TatI | WGTACW | 3 cut(s) | 145, 658, 1428 |
| TfiI | GAWTC | 3 cut(s) | 138, 892, 969 |
| Tru1I | TTAA | 8 cut(s) | 153, 299, 687, 882, 1002, 1422, 1469, 1848 |
| Tru9I | TTAA | 8 cut(s) | 153, 299, 687, 882, 1002, 1422, 1469, 1848 |
| TscAI | CASTG | 2 cut(s) | 229, 894 |
| TseFI | GTSAC | 4 cut(s) | 157, 220, 832, 1820 |
| TseI | GCWGC | 3 cut(s) | 1144, 1195, 1568 |
| Tsp45I | GTSAC | 4 cut(s) | 157, 220, 832, 1820 |
| TspDTI | ATGAA | 8 cut(s) | 129, 420, 753, 986, 1355, 1572, 1641, 1952 |
| TspGWI | ACGGA | 3 cut(s) | 119, 992, 2039 |
| TspRI | CASTG | 2 cut(s) | 229, 894 |
| VneI | GTGCAC | 1 cut(s) | 429 |
| VspI | ATTAAT | 1 cut(s) | 1469 |
| XapI | RAATTY | 2 cut(s) | 116, 698 |
| XbaI | TCTAGA | 1 cut(s) | 1705 |
| XceI | RCATGY | 6 cut(s) | 179, 1202, 1386, 1615, 1644, 1750 |
| XmiI | GTMKAC | 3 cut(s) | 86, 1330, 1645 |
| XspI | CTAG | 5 cut(s) | 99, 573, 749, 1706, 1928 |
| ZraI | GACGTC | 1 cut(s) | 778 |
| Zsp2I | ATGCAT | 2 cut(s) | 985, 1264 |