Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G32000 AT2G32000 |
| fragaria_vesca | FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 |
| malus_domestica | MD04G1023600.v1.1 |
| prunus_persica | Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 |
| pyrus_communis | pycom04g02020 |
| rosa_chinensis | RchiOBHm_Chr4g0412031 |
| rosa_laevigata | RLG00000008327 |
| rosa_multiflora | Rmu_co8041726.1_g000001 Rmu_sc0000711.1_g000051 |
| rosa_roxburghii | Rroxscaffold_1G00042480 Rroxscaffold_5G00346360 Rroxscaffold_5G00356250 Rroxscaffold_5G00356340 |
| rosa_rugosa | Rorug04G0106700 Rorug04G0106800 Rorug04G0106900 |
| rosa_samantha | Rh4AG171100 Rh4BG168800 Rh4BG169700 Rh4CG181900 Rh4DG164200 Rh4DG165300 |
| rosa_wichuraiana | Rw0G007770 Rw0G008800 Rw4G014260 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 2 cut(s) | 90, 1186 |
| AccB1I | GGYRCC | 2 cut(s) | 90, 1186 |
| AclI | AACGTT | 1 cut(s) | 960 |
| AcsI | RAATTY | 7 cut(s) | 133, 262, 319, 373, 808, 1216, 1292 |
| AfaI | GTAC | 6 cut(s) | 92, 105, 746, 1188, 1302, 1307 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1108 |
| AflII | CTTAAG | 1 cut(s) | 656 |
| AjnI | CCWGG | 1 cut(s) | 1244 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 23, 55 |
| Alw21I | GWGCWC | 2 cut(s) | 617, 1203 |
| Ama87I | CYCGRG | 1 cut(s) | 1202 |
| ApeKI | GCWGC | 4 cut(s) | 14, 1223, 1241, 1251 |
| ApoI | RAATTY | 7 cut(s) | 133, 262, 319, 373, 808, 1216, 1292 |
| Asp718I | GGTACC | 2 cut(s) | 90, 1186 |
| AspLEI | GCGC | 4 cut(s) | 367, 463, 765, 1251 |
| AsuHPI | GGTGA | 2 cut(s) | 676, 1025 |
| AsuII | TTCGAA | 1 cut(s) | 370 |
| AsuNHI | GCTAGC | 1 cut(s) | 1133 |
| AvaI | CYCGRG | 1 cut(s) | 1202 |
| BaeGI | GKGCMC | 1 cut(s) | 248 |
| BanI | GGYRCC | 2 cut(s) | 90, 1186 |
| BanII | GRGCYC | 2 cut(s) | 617, 1203 |
| BbsI | GAAGAC | 3 cut(s) | 134, 265, 876 |
| Bbv12I | GWGCWC | 2 cut(s) | 617, 1203 |
| BbvI | GCAGC | 4 cut(s) | 26, 1235, 1253, 1263 |
| BccI | CCATC | 5 cut(s) | 18, 208, 1085, 1263, 1278 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1103, 1137, 1212, 1246 |
| BciT130I | CCWGG | 1 cut(s) | 1246 |
| BfaI | CTAG | 4 cut(s) | 1086, 1134, 1179, 1269 |
| BfmI | CTRYAG | 1 cut(s) | 12 |
| BfoI | RGCGCY | 2 cut(s) | 368, 464 |
| BfrI | CTTAAG | 1 cut(s) | 656 |
| BisI | GCNGC | 4 cut(s) | 15, 1224, 1242, 1252 |
| BlsI | GCNGC | 4 cut(s) | 16, 1225, 1243, 1253 |
| BmcAI | AGTACT | 1 cut(s) | 1307 |
| Bme1390I | CCNGG | 1 cut(s) | 1246 |
| BmeT110I | CYCGRG | 1 cut(s) | 1202 |
| BmiI | GGNNCC | 3 cut(s) | 82, 92, 1188 |
| BmrFI | CCNGG | 1 cut(s) | 1246 |
| BmsI | GCATC | 3 cut(s) | 765, 853, 939 |
| BmtI | GCTAGC | 1 cut(s) | 1137 |
| BpiI | GAAGAC | 3 cut(s) | 134, 265, 876 |
| Bpu14I | TTCGAA | 1 cut(s) | 370 |
| BpuEI | CTTGAG | 2 cut(s) | 824, 1176 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1108 |
| Bse1I | ACTGG | 1 cut(s) | 854 |
| Bse3DI | GCAATG | 1 cut(s) | 1214 |
| BseBI | CCWGG | 1 cut(s) | 1246 |
| BseGI | GGATG | 2 cut(s) | 200, 1289 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1108 |
| BseMI | GCAATG | 1 cut(s) | 1214 |
| BseMII | CTCAG | 1 cut(s) | 518 |
| BseNI | ACTGG | 1 cut(s) | 854 |
| BseSI | GKGCMC | 1 cut(s) | 248 |
| BseXI | GCAGC | 4 cut(s) | 26, 1235, 1253, 1263 |
| BsgI | GTGCAG | 3 cut(s) | 512, 734, 987 |
| BshNI | GGYRCC | 2 cut(s) | 90, 1186 |
| BsiHKAI | GWGCWC | 2 cut(s) | 617, 1203 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1202 |
| BslFI | GGGAC | 2 cut(s) | 1041, 1301 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1108 |
| BsmFI | GGGAC | 2 cut(s) | 1041, 1301 |
| BsmI | GAATGC | 1 cut(s) | 1046 |
| BsoBI | CYCGRG | 1 cut(s) | 1202 |
| Bsp119I | TTCGAA | 1 cut(s) | 370 |
| Bsp1286I | GDGCHC | 3 cut(s) | 248, 617, 1203 |
| Bsp1407I | TGTACA | 1 cut(s) | 103 |
| Bsp143I | GATC | 3 cut(s) | 423, 509, 583 |
| BspCNI | CTCAG | 1 cut(s) | 519 |
| BspLI | GGNNCC | 3 cut(s) | 82, 92, 1188 |
| BspMAI | CTGCAG | 1 cut(s) | 16 |
| BspOI | GCTAGC | 1 cut(s) | 1137 |
| BspQI | GCTCTTC | 2 cut(s) | 306, 1023 |
| BspT104I | TTCGAA | 1 cut(s) | 370 |
| BspT107I | GGYRCC | 2 cut(s) | 90, 1186 |
| BspTI | CTTAAG | 1 cut(s) | 656 |
| BsrDI | GCAATG | 1 cut(s) | 1214 |
| BsrGI | TGTACA | 1 cut(s) | 103 |
| BsrI | ACTGG | 1 cut(s) | 854 |
| BssMI | GATC | 3 cut(s) | 423, 509, 583 |
| Bst2UI | CCWGG | 1 cut(s) | 1246 |
| Bst4CI | ACNGT | 6 cut(s) | 95, 604, 664, 1093, 1300, 1305 |
| Bst6I | CTCTTC | 4 cut(s) | 306, 882, 920, 1023 |
| BstAFI | CTTAAG | 1 cut(s) | 656 |
| BstAUI | TGTACA | 1 cut(s) | 103 |
| BstBI | TTCGAA | 1 cut(s) | 370 |
| BstC8I | GCNNGC | 4 cut(s) | 197, 227, 410, 1135 |
| BstDEI | CTNAG | 2 cut(s) | 527, 1127 |
| BstF5I | GGATG | 2 cut(s) | 200, 1289 |
| BstH2I | RGCGCY | 2 cut(s) | 368, 464 |
| BstHHI | GCGC | 4 cut(s) | 367, 463, 765, 1251 |
| BstKTI | GATC | 3 cut(s) | 426, 512, 586 |
| BstMBI | GATC | 3 cut(s) | 423, 509, 583 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 762, 850, 1130 |
| BstNI | CCWGG | 1 cut(s) | 1246 |
| BstNSI | RCATGY | 1 cut(s) | 412 |
| BstSCI | CCNGG | 1 cut(s) | 1244 |
| BstSFI | CTRYAG | 1 cut(s) | 12 |
| BstSLI | GKGCMC | 1 cut(s) | 248 |
| BstV1I | GCAGC | 4 cut(s) | 26, 1235, 1253, 1263 |
| BstV2I | GAAGAC | 3 cut(s) | 134, 265, 876 |
| BtgZI | GCGATG | 1 cut(s) | 916 |
| BtsCI | GGATG | 2 cut(s) | 200, 1289 |
| BtsI | GCAGTG | 1 cut(s) | 1046 |
| BtsIMutI | CAGTG | 2 cut(s) | 609, 1046 |
| Cac8I | GCNNGC | 4 cut(s) | 197, 227, 410, 1135 |
| CfoI | GCGC | 4 cut(s) | 367, 463, 765, 1251 |
| Csp6I | GTAC | 6 cut(s) | 91, 104, 745, 1187, 1301, 1306 |
| CviAII | CATG | 4 cut(s) | 409, 457, 760, 936 |
| CviQI | GTAC | 6 cut(s) | 91, 104, 745, 1187, 1301, 1306 |
| DdeI | CTNAG | 2 cut(s) | 527, 1127 |
| DpnI | GATC | 3 cut(s) | 425, 511, 585 |
| DpnII | GATC | 3 cut(s) | 423, 509, 583 |
| Eam1104I | CTCTTC | 4 cut(s) | 306, 882, 920, 1023 |
| EarI | CTCTTC | 4 cut(s) | 306, 882, 920, 1023 |
| Ecl136II | GAGCTC | 2 cut(s) | 615, 1201 |
| Eco24I | GRGCYC | 2 cut(s) | 617, 1203 |
| Eco32I | GATATC | 1 cut(s) | 444 |
| Eco53kI | GAGCTC | 2 cut(s) | 615, 1201 |
| Eco88I | CYCGRG | 1 cut(s) | 1202 |
| EcoICRI | GAGCTC | 2 cut(s) | 615, 1201 |
| EcoRI | GAATTC | 1 cut(s) | 1216 |
| EcoRII | CCWGG | 1 cut(s) | 1244 |
| EcoRV | GATATC | 1 cut(s) | 444 |
| EcoT22I | ATGCAT | 1 cut(s) | 414 |
| EcoT38I | GRGCYC | 2 cut(s) | 617, 1203 |
| FaeI | CATG | 4 cut(s) | 412, 460, 763, 939 |
| FaqI | GGGAC | 2 cut(s) | 1041, 1301 |
| FatI | CATG | 4 cut(s) | 408, 456, 759, 935 |
| FauNDI | CATATG | 1 cut(s) | 160 |
| Fnu4HI | GCNGC | 4 cut(s) | 15, 1224, 1242, 1252 |
| FokI | GGATG | 2 cut(s) | 187, 1296 |
| FriOI | GRGCYC | 2 cut(s) | 617, 1203 |
| Fsp4HI | GCNGC | 4 cut(s) | 15, 1224, 1242, 1252 |
| FspBI | CTAG | 4 cut(s) | 1086, 1134, 1179, 1269 |
| GlaI | GCGC | 4 cut(s) | 366, 462, 764, 1250 |
| GluI | GCNGC | 4 cut(s) | 15, 1224, 1242, 1252 |
| HaeII | RGCGCY | 2 cut(s) | 368, 464 |
| HhaI | GCGC | 4 cut(s) | 367, 463, 765, 1251 |
| Hin1II | CATG | 4 cut(s) | 412, 460, 763, 939 |
| Hin6I | GCGC | 4 cut(s) | 365, 461, 763, 1249 |
| HinP1I | GCGC | 4 cut(s) | 365, 461, 763, 1249 |
| HincII | GTYRAC | 1 cut(s) | 597 |
| HindII | GTYRAC | 1 cut(s) | 597 |
| HindIII | AAGCTT | 1 cut(s) | 518 |
| HinfI | GANTC | 2 cut(s) | 497, 667 |
| HphI | GGTGA | 2 cut(s) | 676, 1025 |
| Hpy166II | GTNNAC | 1 cut(s) | 597 |
| Hpy188III | TCNNGA | 2 cut(s) | 336, 671 |
| Hpy8I | GTNNAC | 1 cut(s) | 597 |
| HpyCH4III | ACNGT | 6 cut(s) | 95, 604, 664, 1093, 1300, 1305 |
| HpyCH4IV | ACGT | 1 cut(s) | 960 |
| HpyCH4V | TGCA | 8 cut(s) | 14, 235, 412, 493, 628, 715, 968, 1039 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 762, 850, 1130 |
| HpyF3I | CTNAG | 2 cut(s) | 527, 1127 |
| HpySE526I | ACGT | 1 cut(s) | 960 |
| Hsp92II | CATG | 4 cut(s) | 412, 460, 763, 939 |
| HspAI | GCGC | 4 cut(s) | 365, 461, 763, 1249 |
| KpnI | GGTACC | 2 cut(s) | 94, 1190 |
| Kzo9I | GATC | 3 cut(s) | 423, 509, 583 |
| LguI | GCTCTTC | 2 cut(s) | 306, 1023 |
| LmnI | GCTCC | 1 cut(s) | 80 |
| LpnPI | CCDG | 7 cut(s) | 3, 418, 479, 867, 1231, 1244, 1258 |
| Lsp1109I | GCAGC | 4 cut(s) | 26, 1235, 1253, 1263 |
| LweI | GCATC | 3 cut(s) | 765, 853, 939 |
| MaeI | CTAG | 4 cut(s) | 1086, 1134, 1179, 1269 |
| MaeII | ACGT | 1 cut(s) | 960 |
| MaeIII | GTNAC | 4 cut(s) | 313, 345, 604, 1067 |
| MalI | GATC | 3 cut(s) | 425, 511, 585 |
| MboI | GATC | 3 cut(s) | 423, 509, 583 |
| MhlI | GDGCHC | 3 cut(s) | 248, 617, 1203 |
| MmeI | TCCRAC | 3 cut(s) | 21, 104, 228 |
| MnlI | CCTC | 9 cut(s) | 143, 522, 663, 883, 1004, 1069, 1108, 1177, 1200 |
| Mph1103I | ATGCAT | 1 cut(s) | 414 |
| MseI | TTAA | 5 cut(s) | 377, 480, 522, 657, 1004 |
| MslI | CAYNNNNRTG | 3 cut(s) | 309, 1051, 1278 |
| MspCI | CTTAAG | 1 cut(s) | 656 |
| MspR9I | CCNGG | 1 cut(s) | 1246 |
| Mva1269I | GAATGC | 1 cut(s) | 1046 |
| MvaI | CCWGG | 1 cut(s) | 1246 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 762, 850, 1130 |
| NdeI | CATATG | 1 cut(s) | 160 |
| NdeII | GATC | 3 cut(s) | 423, 509, 583 |
| NheI | GCTAGC | 1 cut(s) | 1133 |
| NlaIII | CATG | 4 cut(s) | 412, 460, 763, 939 |
| NlaIV | GGNNCC | 3 cut(s) | 82, 92, 1188 |
| NmuCI | GTSAC | 4 cut(s) | 313, 345, 604, 1067 |
| NsiI | ATGCAT | 1 cut(s) | 414 |
| NspI | RCATGY | 1 cut(s) | 412 |
| NspV | TTCGAA | 1 cut(s) | 370 |
| PaeI | GCATGC | 1 cut(s) | 412 |
| PaeR7I | CTCGAG | 1 cut(s) | 1202 |
| PciSI | GCTCTTC | 2 cut(s) | 306, 1023 |
| PctI | GAATGC | 1 cut(s) | 1046 |
| PfeI | GAWTC | 2 cut(s) | 497, 667 |
| PkrI | GCNGC | 4 cut(s) | 16, 1225, 1243, 1253 |
| Psp124BI | GAGCTC | 2 cut(s) | 617, 1203 |
| Psp1406I | AACGTT | 1 cut(s) | 960 |
| Psp6I | CCWGG | 1 cut(s) | 1244 |
| PspGI | CCWGG | 1 cut(s) | 1244 |
| PspN4I | GGNNCC | 3 cut(s) | 82, 92, 1188 |
| PspXI | VCTCGAGB | 1 cut(s) | 1202 |
| PstI | CTGCAG | 1 cut(s) | 16 |
| RsaI | GTAC | 6 cut(s) | 92, 105, 746, 1188, 1302, 1307 |
| RsaNI | GTAC | 6 cut(s) | 91, 104, 745, 1187, 1301, 1306 |
| RseI | CAYNNNNRTG | 3 cut(s) | 309, 1051, 1278 |
| SacI | GAGCTC | 2 cut(s) | 617, 1203 |
| SapI | GCTCTTC | 2 cut(s) | 306, 1023 |
| SaqAI | TTAA | 5 cut(s) | 377, 480, 522, 657, 1004 |
| SatI | GCNGC | 4 cut(s) | 15, 1224, 1242, 1252 |
| Sau3AI | GATC | 3 cut(s) | 423, 509, 583 |
| ScaI | AGTACT | 1 cut(s) | 1307 |
| ScrFI | CCNGG | 1 cut(s) | 1246 |
| SduI | GDGCHC | 3 cut(s) | 248, 617, 1203 |
| SfaNI | GCATC | 3 cut(s) | 765, 853, 939 |
| SfcI | CTRYAG | 1 cut(s) | 12 |
| Sfr274I | CTCGAG | 1 cut(s) | 1202 |
| SfuI | TTCGAA | 1 cut(s) | 370 |
| SlaI | CTCGAG | 1 cut(s) | 1202 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 309, 1051, 1278 |
| SmlI | CTYRAG | 4 cut(s) | 656, 803, 1191, 1202 |
| SmoI | CTYRAG | 4 cut(s) | 656, 803, 1191, 1202 |
| SphI | GCATGC | 1 cut(s) | 412 |
| SspMI | CTAG | 4 cut(s) | 1086, 1134, 1179, 1269 |
| SstI | GAGCTC | 2 cut(s) | 617, 1203 |
| StyD4I | CCNGG | 1 cut(s) | 1244 |
| TaaI | ACNGT | 6 cut(s) | 95, 604, 664, 1093, 1300, 1305 |
| TaiI | ACGT | 1 cut(s) | 963 |
| TaqI | TCGA | 6 cut(s) | 148, 276, 370, 1113, 1203, 1232 |
| TatI | WGTACW | 4 cut(s) | 103, 744, 1300, 1305 |
| TfiI | GAWTC | 2 cut(s) | 497, 667 |
| Tru1I | TTAA | 5 cut(s) | 377, 480, 522, 657, 1004 |
| Tru9I | TTAA | 5 cut(s) | 377, 480, 522, 657, 1004 |
| TscAI | CASTG | 2 cut(s) | 609, 1046 |
| TseFI | GTSAC | 4 cut(s) | 313, 345, 604, 1067 |
| TseI | GCWGC | 4 cut(s) | 14, 1223, 1241, 1251 |
| Tsp45I | GTSAC | 4 cut(s) | 313, 345, 604, 1067 |
| TspDTI | ATGAA | 7 cut(s) | 17, 177, 273, 293, 312, 563, 907 |
| TspRI | CASTG | 2 cut(s) | 609, 1046 |
| Vha464I | CTTAAG | 1 cut(s) | 656 |
| XapI | RAATTY | 7 cut(s) | 133, 262, 319, 373, 808, 1216, 1292 |
| XceI | RCATGY | 1 cut(s) | 412 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 165 |
| XhoI | CTCGAG | 1 cut(s) | 1202 |
| XspI | CTAG | 4 cut(s) | 1086, 1134, 1179, 1269 |
| ZrmI | AGTACT | 1 cut(s) | 1307 |
| Zsp2I | ATGCAT | 1 cut(s) | 414 |