Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G32000 AT2G32000 |
| fragaria_vesca | FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 |
| malus_domestica | MD04G1023600.v1.1 |
| prunus_persica | Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 |
| pyrus_communis | pycom04g02020 |
| rosa_chinensis | RchiOBHm_Chr4g0412031 |
| rosa_laevigata | RLG00000008327 |
| rosa_multiflora | Rmu_co8041726.1_g000001 Rmu_sc0000711.1_g000051 |
| rosa_roxburghii | Rroxscaffold_1G00042480 Rroxscaffold_5G00346360 Rroxscaffold_5G00356250 Rroxscaffold_5G00356340 |
| rosa_rugosa | Rorug04G0106700 Rorug04G0106800 Rorug04G0106900 |
| rosa_samantha | Rh4AG171100 Rh4BG168800 Rh4BG169700 Rh4CG181900 Rh4DG164200 Rh4DG165300 |
| rosa_wichuraiana | Rw0G007770 Rw0G008800 Rw4G014260 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 1442 |
| Acc16I | TGCGCA | 1 cut(s) | 1047 |
| Acc36I | ACCTGC | 1 cut(s) | 1549 |
| Acc65I | GGTACC | 1 cut(s) | 2555 |
| AccB1I | GGYRCC | 2 cut(s) | 2337, 2555 |
| AccBSI | CCGCTC | 1 cut(s) | 2335 |
| AccI | GTMKAC | 5 cut(s) | 86, 965, 1858, 2173, 2417 |
| AciI | CCGC | 3 cut(s) | 2260, 2335, 2551 |
| AclWI | GGATC | 6 cut(s) | 221, 1110, 1625, 2527, 2567, 2580 |
| AcoI | YGGCCR | 2 cut(s) | 394, 939 |
| AcsI | RAATTY | 4 cut(s) | 116, 822, 1274, 1292 |
| AcuI | CTGAAG | 2 cut(s) | 728, 920 |
| AcyI | GRCGYC | 1 cut(s) | 1439 |
| AdeI | CACNNNGTG | 1 cut(s) | 1043 |
| AfeI | AGCGCT | 1 cut(s) | 2492 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 647, 1066, 1067, 1118, 1429, 1940, 2072, 2259 |
| AflIII | ACRYGT | 1 cut(s) | 2168 |
| AgeI | ACCGGT | 1 cut(s) | 2420 |
| AjnI | CCWGG | 1 cut(s) | 2011 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1177, 1209 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2214, 2246 |
| Alw21I | GWGCWC | 5 cut(s) | 433, 919, 1004, 2251, 2311 |
| Alw26I | GTCTC | 3 cut(s) | 401, 1192, 2402 |
| Alw44I | GTGCAC | 1 cut(s) | 915 |
| AlwI | GGATC | 6 cut(s) | 221, 1110, 1625, 2527, 2567, 2580 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1087, 1364 |
| Ama87I | CYCGRG | 1 cut(s) | 2328 |
| Aor51HI | AGCGCT | 1 cut(s) | 2492 |
| AoxI | GGCC | 5 cut(s) | 394, 468, 626, 939, 2014 |
| ApaLI | GTGCAC | 1 cut(s) | 915 |
| ApeKI | GCWGC | 2 cut(s) | 1087, 1672 |
| ApoI | RAATTY | 4 cut(s) | 116, 822, 1274, 1292 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2072, 2104 |
| AsiGI | ACCGGT | 1 cut(s) | 2420 |
| Asp718I | GGTACC | 1 cut(s) | 2555 |
| AspLEI | GCGC | 3 cut(s) | 1048, 1548, 2493 |
| AspS9I | GGNCC | 6 cut(s) | 626, 929, 1420, 1583, 2015, 2108 |
| AsuC2I | CCSGG | 3 cut(s) | 1120, 2018, 2560 |
| AsuHPI | GGTGA | 4 cut(s) | 1145, 1336, 1434, 2303 |
| AvaI | CYCGRG | 1 cut(s) | 2328 |
| AvaII | GGWCC | 4 cut(s) | 929, 1420, 1583, 2108 |
| AxyI | CCTNAGG | 1 cut(s) | 1594 |
| BaeGI | GKGCMC | 2 cut(s) | 919, 2342 |
| BalI | TGGCCA | 2 cut(s) | 396, 941 |
| BamHI | GGATCC | 1 cut(s) | 2572 |
| BanI | GGYRCC | 2 cut(s) | 2337, 2555 |
| BanII | GRGCYC | 1 cut(s) | 2311 |
| BauI | CACGAG | 1 cut(s) | 312 |
| BbsI | GAAGAC | 1 cut(s) | 2390 |
| Bbv12I | GWGCWC | 5 cut(s) | 433, 919, 1004, 2251, 2311 |
| BbvI | GCAGC | 2 cut(s) | 1074, 1684 |
| BccI | CCATC | 6 cut(s) | 812, 931, 1091, 1146, 2015, 2119 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 1648, 1682, 2319, 2353 |
| BciT130I | CCWGG | 1 cut(s) | 2013 |
| BcnI | CCSGG | 3 cut(s) | 1120, 2018, 2560 |
| BcoDI | GTCTC | 3 cut(s) | 401, 1192, 2402 |
| BfmI | CTRYAG | 2 cut(s) | 54, 671 |
| BfoI | RGCGCY | 1 cut(s) | 2494 |
| BfuAI | ACCTGC | 1 cut(s) | 1549 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2022 |
| BglII | AGATCT | 1 cut(s) | 1636 |
| BisI | GCNGC | 3 cut(s) | 1088, 1673, 2261 |
| BlsI | GCNGC | 3 cut(s) | 1089, 1674, 2262 |
| Bme1390I | CCNGG | 4 cut(s) | 1120, 2013, 2018, 2560 |
| Bme18I | GGWCC | 4 cut(s) | 929, 1420, 1583, 2108 |
| BmeT110I | CYCGRG | 1 cut(s) | 2328 |
| BmgT120I | GGNCC | 6 cut(s) | 626, 929, 1420, 1583, 2015, 2108 |
| BmiI | GGNNCC | 4 cut(s) | 1584, 2339, 2557, 2574 |
| BmrFI | CCNGG | 4 cut(s) | 1120, 2013, 2018, 2560 |
| BmsI | GCATC | 8 cut(s) | 304, 508, 1180, 1321, 1498, 1635, 1777, 2130 |
| BpiI | GAAGAC | 1 cut(s) | 2390 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2336, 2368 |
| BpmI | CTGGAG | 1 cut(s) | 1431 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1692 |
| BpuEI | CTTGAG | 1 cut(s) | 954 |
| BpuMI | CCSGG | 3 cut(s) | 1120, 2018, 2560 |
| Bsa29I | ATCGAT | 1 cut(s) | 1629 |
| BsaAI | YACGTR | 1 cut(s) | 992 |
| BsaBI | GATNNNNATC | 3 cut(s) | 348, 1101, 1635 |
| BsaHI | GRCGYC | 1 cut(s) | 1439 |
| BsaJI | CCNNGG | 6 cut(s) | 73, 471, 646, 1349, 2191, 2394 |
| BsaWI | WCCGGW | 2 cut(s) | 691, 2420 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1225, 1255, 1429, 1459 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 647, 1066, 1067, 1118, 1429, 1940, 2072, 2259 |
| Bse118I | RCCGGY | 1 cut(s) | 2420 |
| Bse21I | CCTNAGG | 1 cut(s) | 1594 |
| Bse8I | GATNNNNATC | 3 cut(s) | 348, 1101, 1635 |
| BseBI | CCWGG | 1 cut(s) | 2013 |
| BseCI | ATCGAT | 1 cut(s) | 1629 |
| BseDI | CCNNGG | 6 cut(s) | 73, 471, 646, 1349, 2191, 2394 |
| BseGI | GGATG | 8 cut(s) | 112, 505, 523, 1138, 1440, 1792, 2026, 2145 |
| BseJI | GATNNNNATC | 3 cut(s) | 348, 1101, 1635 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 647, 1066, 1067, 1118, 1429, 1940, 2072, 2259 |
| BseMII | CTCAG | 9 cut(s) | 290, 441, 828, 1161, 1356, 1394, 1839, 1852, 2334 |
| BseRI | GAGGAG | 1 cut(s) | 1931 |
| BseSI | GKGCMC | 2 cut(s) | 919, 2342 |
| BseXI | GCAGC | 2 cut(s) | 1074, 1684 |
| BseYI | CCCAGC | 3 cut(s) | 1579, 2122, 2135 |
| BsgI | GTGCAG | 1 cut(s) | 1577 |
| BshFI | GGCC | 5 cut(s) | 396, 470, 628, 941, 2016 |
| BshNI | GGYRCC | 2 cut(s) | 2337, 2555 |
| BshTI | ACCGGT | 1 cut(s) | 2420 |
| BshVI | ATCGAT | 1 cut(s) | 1629 |
| BsiHKAI | GWGCWC | 5 cut(s) | 433, 919, 1004, 2251, 2311 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2328 |
| BsiSI | CCGG | 5 cut(s) | 692, 1119, 2018, 2421, 2559 |
| BslFI | GGGAC | 2 cut(s) | 1135, 1246 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 647, 1066, 1067, 1118, 1429, 1940, 2072, 2259 |
| BsmAI | GTCTC | 3 cut(s) | 401, 1192, 2402 |
| BsmBI | CGTCTC | 1 cut(s) | 2402 |
| BsmFI | GGGAC | 2 cut(s) | 1135, 1246 |
| BsmI | GAATGC | 1 cut(s) | 2427 |
| BsnI | GGCC | 5 cut(s) | 396, 470, 628, 941, 2016 |
| BsoBI | CYCGRG | 1 cut(s) | 2328 |
| Bsp1286I | GDGCHC | 6 cut(s) | 433, 919, 1004, 2251, 2311, 2342 |
| Bsp1407I | TGTACA | 3 cut(s) | 658, 1217, 1956 |
| Bsp143I | GATC | 9 cut(s) | 226, 235, 349, 1115, 1630, 1636, 1705, 2519, 2572 |
| Bsp19I | CCATGG | 2 cut(s) | 471, 1349 |
| BspACI | CCGC | 3 cut(s) | 2260, 2335, 2551 |
| BspANI | GGCC | 5 cut(s) | 396, 470, 628, 941, 2016 |
| BspCNI | CTCAG | 9 cut(s) | 291, 442, 829, 1162, 1357, 1393, 1838, 1851, 2335 |
| BspDI | ATCGAT | 1 cut(s) | 1629 |
| BspHI | TCATGA | 1 cut(s) | 1426 |
| BspLI | GGNNCC | 4 cut(s) | 1584, 2339, 2557, 2574 |
| BspMAI | CTGCAG | 1 cut(s) | 675 |
| BspMI | ACCTGC | 1 cut(s) | 1549 |
| BspPI | GGATC | 6 cut(s) | 221, 1110, 1625, 2527, 2567, 2580 |
| BspT107I | GGYRCC | 2 cut(s) | 2337, 2555 |
| BsrBI | CCGCTC | 1 cut(s) | 2335 |
| BsrFI | RCCGGY | 1 cut(s) | 2420 |
| BsrGI | TGTACA | 3 cut(s) | 658, 1217, 1956 |
| BssAI | RCCGGY | 1 cut(s) | 2420 |
| BssECI | CCNNGG | 6 cut(s) | 73, 471, 646, 1349, 2191, 2394 |
| BssMI | GATC | 9 cut(s) | 226, 235, 349, 1115, 1630, 1636, 1705, 2519, 2572 |
| BssNAI | GTATAC | 2 cut(s) | 966, 2418 |
| BssNI | GRCGYC | 1 cut(s) | 1439 |
| BssSI | CACGAG | 1 cut(s) | 312 |
| BssT1I | CCWWGG | 5 cut(s) | 471, 646, 1349, 2191, 2394 |
| Bst1107I | GTATAC | 2 cut(s) | 966, 2418 |
| Bst2BI | CACGAG | 1 cut(s) | 312 |
| Bst2UI | CCWGG | 1 cut(s) | 2013 |
| Bst4CI | ACNGT | 5 cut(s) | 564, 889, 1237, 1757, 1863 |
| Bst6I | CTCTTC | 1 cut(s) | 2497 |
| BstACI | GRCGYC | 1 cut(s) | 1439 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 518, 2277 |
| BstAUI | TGTACA | 3 cut(s) | 658, 1217, 1956 |
| BstBAI | YACGTR | 1 cut(s) | 992 |
| BstC8I | GCNNGC | 6 cut(s) | 293, 1092, 1570, 1701, 2120, 2206 |
| BstDSI | CCRYGG | 3 cut(s) | 73, 471, 1349 |
| BstEII | GGTNACC | 1 cut(s) | 1133 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1427, 2070 |
| BstF5I | GGATG | 8 cut(s) | 112, 505, 523, 1138, 1440, 1792, 2026, 2145 |
| BstH2I | RGCGCY | 1 cut(s) | 2494 |
| BstHHI | GCGC | 3 cut(s) | 1048, 1548, 2493 |
| BstKTI | GATC | 9 cut(s) | 229, 238, 352, 1118, 1633, 1639, 1708, 2522, 2575 |
| BstMAI | GTCTC | 3 cut(s) | 401, 1192, 2402 |
| BstMBI | GATC | 9 cut(s) | 226, 235, 349, 1115, 1630, 1636, 1705, 2519, 2572 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 518, 914, 1623, 2022, 2049, 2154, 2277, 2452 |
| BstNI | CCWGG | 1 cut(s) | 2013 |
| BstNSI | RCATGY | 5 cut(s) | 179, 1180, 1730, 1914, 2172 |
| BstPI | GGTNACC | 1 cut(s) | 1133 |
| BstSCI | CCNGG | 4 cut(s) | 1118, 2011, 2016, 2558 |
| BstSFI | CTRYAG | 2 cut(s) | 54, 671 |
| BstSLI | GKGCMC | 2 cut(s) | 919, 2342 |
| BstV1I | GCAGC | 2 cut(s) | 1074, 1684 |
| BstV2I | GAAGAC | 1 cut(s) | 2390 |
| BstX2I | RGATCY | 2 cut(s) | 1636, 2572 |
| BstYI | RGATCY | 2 cut(s) | 1636, 2572 |
| BstZ17I | GTATAC | 2 cut(s) | 966, 2418 |
| Bsu15I | ATCGAT | 1 cut(s) | 1629 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1594 |
| BsuRI | GGCC | 5 cut(s) | 396, 470, 628, 941, 2016 |
| BsuTUI | ATCGAT | 1 cut(s) | 1629 |
| BtgI | CCRYGG | 3 cut(s) | 73, 471, 1349 |
| BtsCI | GGATG | 8 cut(s) | 112, 505, 523, 1138, 1440, 1792, 2026, 2145 |
| BtsI | GCAGTG | 1 cut(s) | 519 |
| BtsIMutI | CAGTG | 3 cut(s) | 190, 222, 519 |
| BveI | ACCTGC | 1 cut(s) | 1549 |
| Cac8I | GCNNGC | 6 cut(s) | 293, 1092, 1570, 1701, 2120, 2206 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1087, 1364 |
| CciI | TCATGA | 1 cut(s) | 1426 |
| CfoI | GCGC | 3 cut(s) | 1048, 1548, 2493 |
| Cfr10I | RCCGGY | 1 cut(s) | 2420 |
| Cfr13I | GGNCC | 6 cut(s) | 626, 929, 1420, 1583, 2015, 2108 |
| ClaI | ATCGAT | 1 cut(s) | 1629 |
| CseI | GACGC | 1 cut(s) | 1500 |
| CspAI | ACCGGT | 1 cut(s) | 2420 |
| DpnI | GATC | 9 cut(s) | 228, 237, 351, 1117, 1632, 1638, 1707, 2521, 2574 |
| DpnII | GATC | 9 cut(s) | 226, 235, 349, 1115, 1630, 1636, 1705, 2519, 2572 |
| DraI | TTTAAA | 2 cut(s) | 154, 465 |
| DraIII | CACNNNGTG | 1 cut(s) | 1043 |
| EaeI | YGGCCR | 2 cut(s) | 394, 939 |
| Eam1104I | CTCTTC | 1 cut(s) | 2497 |
| EarI | CTCTTC | 1 cut(s) | 2497 |
| Ecl136II | GAGCTC | 1 cut(s) | 2309 |
| Eco130I | CCWWGG | 5 cut(s) | 471, 646, 1349, 2191, 2394 |
| Eco24I | GRGCYC | 1 cut(s) | 2311 |
| Eco47I | GGWCC | 4 cut(s) | 929, 1420, 1583, 2108 |
| Eco47III | AGCGCT | 1 cut(s) | 2492 |
| Eco53kI | GAGCTC | 1 cut(s) | 2309 |
| Eco57I | CTGAAG | 2 cut(s) | 728, 920 |
| Eco81I | CCTNAGG | 1 cut(s) | 1594 |
| Eco88I | CYCGRG | 1 cut(s) | 2328 |
| Eco91I | GGTNACC | 1 cut(s) | 1133 |
| EcoICRI | GAGCTC | 1 cut(s) | 2309 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1427, 2070 |
| EcoO65I | GGTNACC | 1 cut(s) | 1133 |
| EcoRI | GAATTC | 2 cut(s) | 822, 1292 |
| EcoRII | CCWGG | 1 cut(s) | 2011 |
| EcoT14I | CCWWGG | 5 cut(s) | 471, 646, 1349, 2191, 2394 |
| EcoT22I | ATGCAT | 2 cut(s) | 1628, 1792 |
| EcoT38I | GRGCYC | 1 cut(s) | 2311 |
| ErhI | CCWWGG | 5 cut(s) | 471, 646, 1349, 2191, 2394 |
| Esp3I | CGTCTC | 1 cut(s) | 2402 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 856, 888, 1407, 1439 |
| FaqI | GGGAC | 2 cut(s) | 1135, 1246 |
| FauI | CCCGC | 1 cut(s) | 2544 |
| FauNDI | CATATG | 1 cut(s) | 1538 |
| FblI | GTMKAC | 5 cut(s) | 86, 965, 1858, 2173, 2417 |
| Fnu4HI | GCNGC | 3 cut(s) | 1088, 1673, 2261 |
| FokI | GGATG | 8 cut(s) | 119, 512, 530, 1125, 1447, 1799, 2033, 2152 |
| FriOI | GRGCYC | 1 cut(s) | 2311 |
| Fsp4HI | GCNGC | 3 cut(s) | 1088, 1673, 2261 |
| FspI | TGCGCA | 1 cut(s) | 1047 |
| GlaI | GCGC | 3 cut(s) | 1047, 1547, 2492 |
| GluI | GCNGC | 3 cut(s) | 1088, 1673, 2261 |
| GsaI | CCCAGC | 3 cut(s) | 1583, 2126, 2139 |
| GsuI | CTGGAG | 1 cut(s) | 1431 |
| HaeII | RGCGCY | 1 cut(s) | 2494 |
| HaeIII | GGCC | 5 cut(s) | 396, 470, 628, 941, 2016 |
| HapII | CCGG | 5 cut(s) | 692, 1119, 2018, 2421, 2559 |
| HgaI | GACGC | 1 cut(s) | 1500 |
| HhaI | GCGC | 3 cut(s) | 1048, 1548, 2493 |
| Hin1I | GRCGYC | 1 cut(s) | 1439 |
| Hin6I | GCGC | 3 cut(s) | 1046, 1546, 2491 |
| HinP1I | GCGC | 3 cut(s) | 1046, 1546, 2491 |
| HincII | GTYRAC | 1 cut(s) | 2174 |
| HindII | GTYRAC | 1 cut(s) | 2174 |
| HindIII | AAGCTT | 1 cut(s) | 574 |
| HinfI | GANTC | 5 cut(s) | 605, 637, 1823, 2196, 2351 |
| HpaII | CCGG | 5 cut(s) | 692, 1119, 2018, 2421, 2559 |
| HphI | GGTGA | 4 cut(s) | 1145, 1336, 1434, 2303 |
| Hpy188I | TCNGA | 5 cut(s) | 300, 636, 900, 1083, 2600 |
| HpyCH4III | ACNGT | 5 cut(s) | 564, 889, 1237, 1757, 1863 |
| HpyCH4IV | ACGT | 4 cut(s) | 760, 991, 1439, 2411 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 518, 914, 1623, 2022, 2049, 2154, 2277, 2452 |
| HpySE526I | ACGT | 4 cut(s) | 760, 991, 1439, 2411 |
| Hsp92I | GRCGYC | 1 cut(s) | 1439 |
| HspAI | GCGC | 3 cut(s) | 1046, 1546, 2491 |
| KpnI | GGTACC | 1 cut(s) | 2559 |
| Kzo9I | GATC | 9 cut(s) | 226, 235, 349, 1115, 1630, 1636, 1705, 2519, 2572 |
| LmnI | GCTCC | 5 cut(s) | 2132, 2254, 2301, 2306, 2451 |
| Lsp1109I | GCAGC | 2 cut(s) | 1074, 1684 |
| LweI | GCATC | 8 cut(s) | 304, 508, 1180, 1321, 1498, 1635, 1777, 2130 |
| MaeII | ACGT | 4 cut(s) | 760, 991, 1439, 2411 |
| MalI | GATC | 9 cut(s) | 228, 237, 351, 1117, 1632, 1638, 1707, 2521, 2574 |
| MbiI | CCGCTC | 1 cut(s) | 2335 |
| MboI | GATC | 9 cut(s) | 226, 235, 349, 1115, 1630, 1636, 1705, 2519, 2572 |
| MflI | RGATCY | 2 cut(s) | 1636, 2572 |
| MhlI | GDGCHC | 6 cut(s) | 433, 919, 1004, 2251, 2311, 2342 |
| MlsI | TGGCCA | 2 cut(s) | 396, 941 |
| MluNI | TGGCCA | 2 cut(s) | 396, 941 |
| MlyI | GAGTC | 4 cut(s) | 631, 1817, 2205, 2345 |
| MmeI | TCCRAC | 4 cut(s) | 33, 321, 526, 1610 |
| Mox20I | TGGCCA | 2 cut(s) | 396, 941 |
| Mph1103I | ATGCAT | 2 cut(s) | 1628, 1792 |
| MscI | TGGCCA | 2 cut(s) | 396, 941 |
| MseI | TTAA | 5 cut(s) | 153, 464, 882, 1530, 1950 |
| MslI | CAYNNNNRTG | 2 cut(s) | 402, 1713 |
| Msp20I | TGGCCA | 2 cut(s) | 396, 941 |
| MspA1I | CMGCKG | 1 cut(s) | 2122 |
| MspI | CCGG | 5 cut(s) | 692, 1119, 2018, 2421, 2559 |
| MspR9I | CCNGG | 4 cut(s) | 1120, 2013, 2018, 2560 |
| Mva1269I | GAATGC | 1 cut(s) | 2427 |
| MvaI | CCWGG | 1 cut(s) | 2013 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 518, 914, 1623, 2022, 2049, 2154, 2277, 2452 |
| NciI | CCSGG | 3 cut(s) | 1120, 2018, 2560 |
| NcoI | CCATGG | 2 cut(s) | 471, 1349 |
| NdeI | CATATG | 1 cut(s) | 1538 |
| NdeII | GATC | 9 cut(s) | 226, 235, 349, 1115, 1630, 1636, 1705, 2519, 2572 |
| NlaIV | GGNNCC | 4 cut(s) | 1584, 2339, 2557, 2574 |
| NmeAIII | GCCGAG | 1 cut(s) | 502 |
| NmuCI | GTSAC | 5 cut(s) | 832, 1133, 1440, 2348, 2435 |
| NsbI | TGCGCA | 1 cut(s) | 1047 |
| NsiI | ATGCAT | 2 cut(s) | 1628, 1792 |
| NspI | RCATGY | 5 cut(s) | 179, 1180, 1730, 1914, 2172 |
| PaeR7I | CTCGAG | 1 cut(s) | 2328 |
| PagI | TCATGA | 1 cut(s) | 1426 |
| PciI | ACATGT | 1 cut(s) | 2168 |
| PctI | GAATGC | 1 cut(s) | 2427 |
| PfeI | GAWTC | 1 cut(s) | 605 |
| PfoI | TCCNGGA | 1 cut(s) | 1118 |
| PinAI | ACCGGT | 1 cut(s) | 2420 |
| PkrI | GCNGC | 3 cut(s) | 1089, 1674, 2262 |
| PleI | GAGTC | 4 cut(s) | 631, 1817, 2204, 2345 |
| PpsI | GAGTC | 4 cut(s) | 631, 1817, 2204, 2345 |
| Ppu21I | YACGTR | 1 cut(s) | 992 |
| PscI | ACATGT | 1 cut(s) | 2168 |
| Psp124BI | GAGCTC | 1 cut(s) | 2311 |
| Psp6I | CCWGG | 1 cut(s) | 2011 |
| PspEI | GGTNACC | 1 cut(s) | 1133 |
| PspFI | CCCAGC | 3 cut(s) | 1579, 2122, 2135 |
| PspGI | CCWGG | 1 cut(s) | 2011 |
| PspN4I | GGNNCC | 4 cut(s) | 1584, 2339, 2557, 2574 |
| PspPI | GGNCC | 6 cut(s) | 626, 929, 1420, 1583, 2015, 2108 |
| PstI | CTGCAG | 1 cut(s) | 675 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1087, 1364 |
| PsuI | RGATCY | 2 cut(s) | 1636, 2572 |
| PvuII | CAGCTG | 1 cut(s) | 2122 |
| RseI | CAYNNNNRTG | 2 cut(s) | 402, 1713 |
| SacI | GAGCTC | 1 cut(s) | 2311 |
| SalI | GTCGAC | 1 cut(s) | 2172 |
| SaqAI | TTAA | 5 cut(s) | 153, 464, 882, 1530, 1950 |
| SatI | GCNGC | 3 cut(s) | 1088, 1673, 2261 |
| Sau3AI | GATC | 9 cut(s) | 226, 235, 349, 1115, 1630, 1636, 1705, 2519, 2572 |
| Sau96I | GGNCC | 6 cut(s) | 626, 929, 1420, 1583, 2015, 2108 |
| SchI | GAGTC | 4 cut(s) | 631, 1817, 2205, 2345 |
| ScrFI | CCNGG | 4 cut(s) | 1120, 2013, 2018, 2560 |
| SduI | GDGCHC | 6 cut(s) | 433, 919, 1004, 2251, 2311, 2342 |
| SfaNI | GCATC | 8 cut(s) | 304, 508, 1180, 1321, 1498, 1635, 1777, 2130 |
| SfcI | CTRYAG | 2 cut(s) | 54, 671 |
| Sfr274I | CTCGAG | 1 cut(s) | 2328 |
| SinI | GGWCC | 4 cut(s) | 929, 1420, 1583, 2108 |
| SlaI | CTCGAG | 1 cut(s) | 2328 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 402, 1713 |
| SmlI | CTYRAG | 2 cut(s) | 969, 2328 |
| SmoI | CTYRAG | 2 cut(s) | 969, 2328 |
| SsiI | CCGC | 3 cut(s) | 2260, 2335, 2551 |
| SspI | AATATT | 1 cut(s) | 367 |
| SstI | GAGCTC | 1 cut(s) | 2311 |
| StyD4I | CCNGG | 4 cut(s) | 1118, 2011, 2016, 2558 |
| StyI | CCWWGG | 5 cut(s) | 471, 646, 1349, 2191, 2394 |
| TaaI | ACNGT | 5 cut(s) | 564, 889, 1237, 1757, 1863 |
| TaiI | ACGT | 4 cut(s) | 763, 994, 1442, 2414 |
| TaqI | TCGA | 5 cut(s) | 1629, 1668, 2079, 2173, 2329 |
| TatI | WGTACW | 4 cut(s) | 658, 1209, 1217, 1956 |
| TauI | GCSGC | 1 cut(s) | 2263 |
| TfiI | GAWTC | 1 cut(s) | 605 |
| Tru1I | TTAA | 5 cut(s) | 153, 464, 882, 1530, 1950 |
| Tru9I | TTAA | 5 cut(s) | 153, 464, 882, 1530, 1950 |
| TscAI | CASTG | 3 cut(s) | 190, 229, 519 |
| TseFI | GTSAC | 5 cut(s) | 832, 1133, 1440, 2348, 2435 |
| TseI | GCWGC | 2 cut(s) | 1087, 1672 |
| Tsp45I | GTSAC | 5 cut(s) | 832, 1133, 1440, 2348, 2435 |
| TspGWI | ACGGA | 3 cut(s) | 119, 609, 2579 |
| TspRI | CASTG | 3 cut(s) | 190, 229, 519 |
| VneI | GTGCAC | 1 cut(s) | 915 |
| VpaK11BI | GGWCC | 4 cut(s) | 929, 1420, 1583, 2108 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1427, 2070 |
| XapI | RAATTY | 4 cut(s) | 116, 822, 1274, 1292 |
| XbaI | TCTAGA | 2 cut(s) | 826, 2233 |
| XceI | RCATGY | 5 cut(s) | 179, 1180, 1730, 1914, 2172 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 311 |
| XhoI | CTCGAG | 1 cut(s) | 2328 |
| XmiI | GTMKAC | 5 cut(s) | 86, 965, 1858, 2173, 2417 |
| ZraI | GACGTC | 1 cut(s) | 1440 |
| Zsp2I | ATGCAT | 2 cut(s) | 1628, 1792 |