Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G32000 AT2G32000 |
| fragaria_vesca | FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 |
| malus_domestica | MD04G1023600.v1.1 |
| prunus_persica | Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 |
| pyrus_communis | pycom04g02020 |
| rosa_chinensis | RchiOBHm_Chr4g0412031 |
| rosa_laevigata | RLG00000008327 |
| rosa_multiflora | Rmu_co8041726.1_g000001 Rmu_sc0000711.1_g000051 |
| rosa_roxburghii | Rroxscaffold_1G00042480 Rroxscaffold_5G00346360 Rroxscaffold_5G00356250 Rroxscaffold_5G00356340 |
| rosa_rugosa | Rorug04G0106700 Rorug04G0106800 Rorug04G0106900 |
| rosa_samantha | Rh4AG171100 Rh4BG168800 Rh4BG169700 Rh4CG181900 Rh4DG164200 Rh4DG165300 |
| rosa_wichuraiana | Rw0G007770 Rw0G008800 Rw4G014260 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 941 |
| AatII | GACGTC | 1 cut(s) | 600 |
| Acc36I | ACCTGC | 2 cut(s) | 941, 1369 |
| Acc65I | GGTACC | 1 cut(s) | 1763 |
| AccB1I | GGYRCC | 1 cut(s) | 1763 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 938 |
| AccBSI | CCGCTC | 1 cut(s) | 2155 |
| AccI | GTMKAC | 3 cut(s) | 785, 1678, 1993 |
| AccII | CGCG | 1 cut(s) | 811 |
| AciI | CCGC | 4 cut(s) | 809, 1477, 2155, 2359 |
| AclWI | GGATC | 6 cut(s) | 41, 63, 1445, 2341, 2375, 2388 |
| AcoI | YGGCCR | 1 cut(s) | 214 |
| AcsI | RAATTY | 2 cut(s) | 518, 1094 |
| AcuI | CTGAAG | 1 cut(s) | 548 |
| AcyI | GRCGYC | 1 cut(s) | 597 |
| AfaI | GTAC | 8 cut(s) | 480, 531, 814, 900, 1383, 1765, 2291, 2365 |
| AfeI | AGCGCT | 1 cut(s) | 485 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 467, 757, 886, 887, 938, 1249, 1760, 1892, 2125 |
| AflIII | ACRYGT | 3 cut(s) | 1135, 1988, 2094 |
| AhlI | ACTAGT | 1 cut(s) | 2275 |
| AjnI | CCWGG | 1 cut(s) | 1831 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1086, 1118 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 2034, 2066 |
| Alw21I | GWGCWC | 5 cut(s) | 253, 739, 824, 2071, 2115 |
| Alw26I | GTCTC | 1 cut(s) | 1721 |
| Alw44I | GTGCAC | 2 cut(s) | 249, 735 |
| AlwI | GGATC | 6 cut(s) | 41, 63, 1445, 2341, 2375, 2388 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 44, 907 |
| Ama87I | CYCGRG | 1 cut(s) | 132 |
| Aor51HI | AGCGCT | 1 cut(s) | 485 |
| AoxI | GGCC | 5 cut(s) | 214, 288, 446, 748, 1834 |
| ApaLI | GTGCAC | 2 cut(s) | 249, 735 |
| ApeKI | GCWGC | 4 cut(s) | 907, 1492, 1543, 1916 |
| ApoI | RAATTY | 2 cut(s) | 518, 1094 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1892, 1924 |
| AseI | ATTAAT | 1 cut(s) | 1817 |
| Asp718I | GGTACC | 1 cut(s) | 1763 |
| AspLEI | GCGC | 2 cut(s) | 486, 1368 |
| AspS9I | GGNCC | 4 cut(s) | 446, 749, 1240, 1835 |
| AsuC2I | CCSGG | 1 cut(s) | 881 |
| AsuHPI | GGTGA | 3 cut(s) | 965, 1156, 1254 |
| AvaI | CYCGRG | 1 cut(s) | 132 |
| AvaII | GGWCC | 1 cut(s) | 1240 |
| AxyI | CCTNAGG | 1 cut(s) | 1414 |
| BaeGI | GKGCMC | 4 cut(s) | 253, 739, 868, 1796 |
| BalI | TGGCCA | 1 cut(s) | 216 |
| BamHI | GGATCC | 1 cut(s) | 2380 |
| BanI | GGYRCC | 1 cut(s) | 1763 |
| BanII | GRGCYC | 1 cut(s) | 2131 |
| Bbv12I | GWGCWC | 5 cut(s) | 253, 739, 824, 2071, 2115 |
| BbvI | GCAGC | 4 cut(s) | 894, 1504, 1555, 1903 |
| BccI | CCATC | 3 cut(s) | 632, 1835, 2216 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2070, 2104 |
| BciT130I | CCWGG | 1 cut(s) | 1833 |
| BclI | TGATCA | 1 cut(s) | 955 |
| BcnI | CCSGG | 1 cut(s) | 881 |
| BcoDI | GTCTC | 1 cut(s) | 1721 |
| BcuI | ACTAGT | 1 cut(s) | 2275 |
| BfaI | CTAG | 4 cut(s) | 569, 740, 2054, 2276 |
| BfmI | CTRYAG | 1 cut(s) | 333 |
| BfoI | RGCGCY | 1 cut(s) | 487 |
| BfuAI | ACCTGC | 2 cut(s) | 941, 1369 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1842 |
| BglII | AGATCT | 1 cut(s) | 1456 |
| BisI | GCNGC | 4 cut(s) | 908, 1493, 1544, 1917 |
| BlsI | GCNGC | 4 cut(s) | 909, 1494, 1545, 1918 |
| Bme1390I | CCNGG | 2 cut(s) | 881, 1833 |
| Bme18I | GGWCC | 1 cut(s) | 1240 |
| BmeT110I | CYCGRG | 1 cut(s) | 132 |
| BmgT120I | GGNCC | 4 cut(s) | 446, 749, 1240, 1835 |
| BmiI | GGNNCC | 3 cut(s) | 1404, 1765, 2382 |
| BmrFI | CCNGG | 2 cut(s) | 881, 1833 |
| BmrI | ACTGGG | 2 cut(s) | 583, 2376 |
| BmsI | GCATC | 7 cut(s) | 328, 415, 937, 1000, 1318, 1340, 1597 |
| BmuI | ACTGGG | 2 cut(s) | 583, 2376 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2156, 2188 |
| BpmI | CTGGAG | 3 cut(s) | 54, 1251, 2133 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1512 |
| BpuEI | CTTGAG | 1 cut(s) | 774 |
| BpuMI | CCSGG | 1 cut(s) | 881 |
| BsaAI | YACGTR | 1 cut(s) | 1791 |
| BsaBI | GATNNNNATC | 2 cut(s) | 168, 1455 |
| BsaHI | GRCGYC | 1 cut(s) | 597 |
| BsaJI | CCNNGG | 6 cut(s) | 291, 466, 1169, 2011, 2122, 2214 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 1045, 1075, 1249, 1279, 2273, 2303 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 467, 757, 886, 887, 938, 1249, 1760, 1892, 2125 |
| Bse1I | ACTGG | 5 cut(s) | 38, 71, 578, 2245, 2371 |
| Bse21I | CCTNAGG | 1 cut(s) | 1414 |
| Bse8I | GATNNNNATC | 2 cut(s) | 168, 1455 |
| BseBI | CCWGG | 1 cut(s) | 1833 |
| BseDI | CCNNGG | 6 cut(s) | 291, 466, 1169, 2011, 2122, 2214 |
| BseGI | GGATG | 4 cut(s) | 958, 1333, 1612, 1981 |
| BseJI | GATNNNNATC | 2 cut(s) | 168, 1455 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 467, 757, 886, 887, 938, 1249, 1760, 1892, 2125 |
| BseMII | CTCAG | 5 cut(s) | 981, 1659, 1672, 1769, 2154 |
| BseNI | ACTGG | 5 cut(s) | 38, 71, 578, 2245, 2371 |
| BseRI | GAGGAG | 1 cut(s) | 2105 |
| BseSI | GKGCMC | 4 cut(s) | 253, 739, 868, 1796 |
| BseXI | GCAGC | 4 cut(s) | 894, 1504, 1555, 1903 |
| BseYI | CCCAGC | 2 cut(s) | 1000, 1942 |
| BsgI | GTGCAG | 2 cut(s) | 1397, 1562 |
| Bsh1236I | CGCG | 1 cut(s) | 811 |
| BshFI | GGCC | 5 cut(s) | 216, 290, 448, 750, 1836 |
| BshNI | GGYRCC | 1 cut(s) | 1763 |
| BsiHKAI | GWGCWC | 5 cut(s) | 253, 739, 824, 2071, 2115 |
| BsiHKCI | CYCGRG | 1 cut(s) | 132 |
| BsiSI | CCGG | 1 cut(s) | 881 |
| BslFI | GGGAC | 2 cut(s) | 955, 1066 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 467, 757, 886, 887, 938, 1249, 1760, 1892, 2125 |
| BsmAI | GTCTC | 1 cut(s) | 1721 |
| BsmBI | CGTCTC | 1 cut(s) | 1721 |
| BsmFI | GGGAC | 2 cut(s) | 955, 1066 |
| BsnI | GGCC | 5 cut(s) | 216, 290, 448, 750, 1836 |
| BsoBI | CYCGRG | 1 cut(s) | 132 |
| Bsp1286I | GDGCHC | 8 cut(s) | 253, 739, 824, 868, 1796, 2071, 2115, 2131 |
| Bsp1407I | TGTACA | 2 cut(s) | 478, 898 |
| Bsp19I | CCATGG | 2 cut(s) | 291, 1169 |
| BspACI | CCGC | 4 cut(s) | 809, 1477, 2155, 2359 |
| BspANI | GGCC | 5 cut(s) | 216, 290, 448, 750, 1836 |
| BspCNI | CTCAG | 5 cut(s) | 982, 1658, 1671, 1768, 2155 |
| BspFNI | CGCG | 1 cut(s) | 811 |
| BspHI | TCATGA | 1 cut(s) | 1246 |
| BspLI | GGNNCC | 3 cut(s) | 1404, 1765, 2382 |
| BspMI | ACCTGC | 2 cut(s) | 941, 1369 |
| BspPI | GGATC | 6 cut(s) | 41, 63, 1445, 2341, 2375, 2388 |
| BspT107I | GGYRCC | 1 cut(s) | 1763 |
| BsrBI | CCGCTC | 1 cut(s) | 2155 |
| BsrGI | TGTACA | 2 cut(s) | 478, 898 |
| BsrI | ACTGG | 5 cut(s) | 38, 71, 578, 2245, 2371 |
| BssECI | CCNNGG | 6 cut(s) | 291, 466, 1169, 2011, 2122, 2214 |
| BssNAI | GTATAC | 1 cut(s) | 786 |
| BssNI | GRCGYC | 1 cut(s) | 597 |
| BssT1I | CCWWGG | 6 cut(s) | 291, 466, 1169, 2011, 2122, 2214 |
| Bst1107I | GTATAC | 1 cut(s) | 786 |
| Bst2UI | CCWGG | 1 cut(s) | 1833 |
| Bst4CI | ACNGT | 8 cut(s) | 40, 709, 920, 1057, 1560, 1577, 1683, 1724 |
| Bst6I | CTCTTC | 2 cut(s) | 1731, 2347 |
| BstACI | GRCGYC | 1 cut(s) | 597 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1965, 2097 |
| BstAUI | TGTACA | 2 cut(s) | 478, 898 |
| BstBAI | YACGTR | 1 cut(s) | 1791 |
| BstC8I | GCNNGC | 6 cut(s) | 113, 1390, 1940, 1957, 1961, 2026 |
| BstDEI | CTNAG | 8 cut(s) | 990, 1277, 1414, 1512, 1645, 1658, 1755, 2163 |
| BstDSI | CCRYGG | 2 cut(s) | 291, 1169 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1247 |
| BstF5I | GGATG | 4 cut(s) | 958, 1333, 1612, 1981 |
| BstFNI | CGCG | 1 cut(s) | 811 |
| BstH2I | RGCGCY | 1 cut(s) | 487 |
| BstHHI | GCGC | 2 cut(s) | 486, 1368 |
| BstMAI | GTCTC | 1 cut(s) | 1721 |
| BstNI | CCWGG | 1 cut(s) | 1833 |
| BstNSI | RCATGY | 5 cut(s) | 1550, 1734, 1963, 1992, 2098 |
| BstSCI | CCNGG | 2 cut(s) | 879, 1831 |
| BstSFI | CTRYAG | 1 cut(s) | 333 |
| BstSLI | GKGCMC | 4 cut(s) | 253, 739, 868, 1796 |
| BstUI | CGCG | 1 cut(s) | 811 |
| BstV1I | GCAGC | 4 cut(s) | 894, 1504, 1555, 1903 |
| BstX2I | RGATCY | 3 cut(s) | 55, 1456, 2380 |
| BstYI | RGATCY | 3 cut(s) | 55, 1456, 2380 |
| BstZ17I | GTATAC | 1 cut(s) | 786 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1414 |
| BsuRI | GGCC | 5 cut(s) | 216, 290, 448, 750, 1836 |
| BtgI | CCRYGG | 2 cut(s) | 291, 1169 |
| BtsCI | GGATG | 4 cut(s) | 958, 1333, 1612, 1981 |
| BtsIMutI | CAGTG | 3 cut(s) | 42, 714, 912 |
| BveI | ACCTGC | 2 cut(s) | 941, 1369 |
| Cac8I | GCNNGC | 6 cut(s) | 113, 1390, 1940, 1957, 1961, 2026 |
| CaiI | CAGNNNCTG | 2 cut(s) | 44, 907 |
| CciI | TCATGA | 1 cut(s) | 1246 |
| CfoI | GCGC | 2 cut(s) | 486, 1368 |
| Cfr13I | GGNCC | 4 cut(s) | 446, 749, 1240, 1835 |
| Csp6I | GTAC | 8 cut(s) | 479, 530, 813, 899, 1382, 1764, 2290, 2364 |
| CviQI | GTAC | 8 cut(s) | 479, 530, 813, 899, 1382, 1764, 2290, 2364 |
| DdeI | CTNAG | 8 cut(s) | 990, 1277, 1414, 1512, 1645, 1658, 1755, 2163 |
| DraI | TTTAAA | 1 cut(s) | 508 |
| EaeI | YGGCCR | 1 cut(s) | 214 |
| Eam1104I | CTCTTC | 2 cut(s) | 1731, 2347 |
| EarI | CTCTTC | 2 cut(s) | 1731, 2347 |
| Eco130I | CCWWGG | 6 cut(s) | 291, 466, 1169, 2011, 2122, 2214 |
| Eco24I | GRGCYC | 1 cut(s) | 2131 |
| Eco32I | GATATC | 2 cut(s) | 1474, 2007 |
| Eco47I | GGWCC | 1 cut(s) | 1240 |
| Eco47III | AGCGCT | 1 cut(s) | 485 |
| Eco57I | CTGAAG | 1 cut(s) | 548 |
| Eco81I | CCTNAGG | 1 cut(s) | 1414 |
| Eco88I | CYCGRG | 1 cut(s) | 132 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1247 |
| EcoRII | CCWGG | 1 cut(s) | 1831 |
| EcoRV | GATATC | 2 cut(s) | 1474, 2007 |
| EcoT14I | CCWWGG | 6 cut(s) | 291, 466, 1169, 2011, 2122, 2214 |
| EcoT22I | ATGCAT | 2 cut(s) | 1333, 1612 |
| EcoT38I | GRGCYC | 1 cut(s) | 2131 |
| ErhI | CCWWGG | 6 cut(s) | 291, 466, 1169, 2011, 2122, 2214 |
| Esp3I | CGTCTC | 1 cut(s) | 1721 |
| FaqI | GGGAC | 2 cut(s) | 955, 1066 |
| FauI | CCCGC | 1 cut(s) | 2352 |
| FauNDI | CATATG | 1 cut(s) | 1358 |
| FbaI | TGATCA | 1 cut(s) | 955 |
| FblI | GTMKAC | 3 cut(s) | 785, 1678, 1993 |
| Fnu4HI | GCNGC | 4 cut(s) | 908, 1493, 1544, 1917 |
| FokI | GGATG | 4 cut(s) | 945, 1340, 1619, 1968 |
| FriOI | GRGCYC | 1 cut(s) | 2131 |
| Fsp4HI | GCNGC | 4 cut(s) | 908, 1493, 1544, 1917 |
| FspBI | CTAG | 4 cut(s) | 569, 740, 2054, 2276 |
| GlaI | GCGC | 2 cut(s) | 485, 1367 |
| GluI | GCNGC | 4 cut(s) | 908, 1493, 1544, 1917 |
| GsaI | CCCAGC | 2 cut(s) | 1004, 1946 |
| GsuI | CTGGAG | 3 cut(s) | 54, 1251, 2133 |
| HaeII | RGCGCY | 1 cut(s) | 487 |
| HaeIII | GGCC | 5 cut(s) | 216, 290, 448, 750, 1836 |
| HapII | CCGG | 1 cut(s) | 881 |
| HhaI | GCGC | 2 cut(s) | 486, 1368 |
| Hin1I | GRCGYC | 1 cut(s) | 597 |
| Hin6I | GCGC | 2 cut(s) | 484, 1366 |
| HinP1I | GCGC | 2 cut(s) | 484, 1366 |
| HincII | GTYRAC | 1 cut(s) | 1994 |
| HindII | GTYRAC | 1 cut(s) | 1994 |
| HinfI | GANTC | 4 cut(s) | 712, 1317, 1643, 2171 |
| HpaII | CCGG | 1 cut(s) | 881 |
| HphI | GGTGA | 3 cut(s) | 965, 1156, 1254 |
| Hpy188I | TCNGA | 6 cut(s) | 10, 368, 667, 1565, 1758, 2309 |
| Hpy99I | CGWCG | 1 cut(s) | 602 |
| HpyAV | CCTTC | 9 cut(s) | 170, 280, 702, 843, 1141, 1253, 1719, 1886, 2316 |
| HpyCH4III | ACNGT | 8 cut(s) | 40, 709, 920, 1057, 1560, 1577, 1683, 1724 |
| HpyCH4IV | ACGT | 4 cut(s) | 503, 597, 1135, 1790 |
| HpyF3I | CTNAG | 8 cut(s) | 990, 1277, 1414, 1512, 1645, 1658, 1755, 2163 |
| HpySE526I | ACGT | 4 cut(s) | 503, 597, 1135, 1790 |
| Hsp92I | GRCGYC | 1 cut(s) | 597 |
| HspAI | GCGC | 2 cut(s) | 484, 1366 |
| KpnI | GGTACC | 1 cut(s) | 1767 |
| Ksp22I | TGATCA | 1 cut(s) | 955 |
| LmnI | GCTCC | 6 cut(s) | 73, 577, 1829, 1913, 2074, 2118 |
| Lsp1109I | GCAGC | 4 cut(s) | 894, 1504, 1555, 1903 |
| LweI | GCATC | 7 cut(s) | 328, 415, 937, 1000, 1318, 1340, 1597 |
| MaeI | CTAG | 4 cut(s) | 569, 740, 2054, 2276 |
| MaeII | ACGT | 4 cut(s) | 503, 597, 1135, 1790 |
| MaeIII | GTNAC | 8 cut(s) | 40, 143, 265, 581, 652, 967, 2168, 2262 |
| MbiI | CCGCTC | 1 cut(s) | 2155 |
| MfeI | CAATTG | 1 cut(s) | 1101 |
| MflI | RGATCY | 3 cut(s) | 55, 1456, 2380 |
| MhlI | GDGCHC | 8 cut(s) | 253, 739, 824, 868, 1796, 2071, 2115, 2131 |
| MlsI | TGGCCA | 1 cut(s) | 216 |
| MluNI | TGGCCA | 1 cut(s) | 216 |
| MlyI | GAGTC | 2 cut(s) | 1637, 2165 |
| MmeI | TCCRAC | 3 cut(s) | 346, 1430, 1588 |
| Mox20I | TGGCCA | 1 cut(s) | 216 |
| Mph1103I | ATGCAT | 2 cut(s) | 1333, 1612 |
| MscI | TGGCCA | 1 cut(s) | 216 |
| MseI | TTAA | 7 cut(s) | 119, 507, 702, 1350, 1770, 1817, 2196 |
| MslI | CAYNNNNRTG | 3 cut(s) | 222, 1533, 2186 |
| Msp20I | TGGCCA | 1 cut(s) | 216 |
| MspI | CCGG | 1 cut(s) | 881 |
| MspR9I | CCNGG | 2 cut(s) | 881, 1833 |
| MssI | GTTTAAAC | 1 cut(s) | 508 |
| MunI | CAATTG | 1 cut(s) | 1101 |
| MvaI | CCWGG | 1 cut(s) | 1833 |
| MvnI | CGCG | 1 cut(s) | 811 |
| NciI | CCSGG | 1 cut(s) | 881 |
| NcoI | CCATGG | 2 cut(s) | 291, 1169 |
| NdeI | CATATG | 1 cut(s) | 1358 |
| NlaIV | GGNNCC | 3 cut(s) | 1404, 1765, 2382 |
| NmuCI | GTSAC | 3 cut(s) | 40, 652, 2168 |
| NsiI | ATGCAT | 2 cut(s) | 1333, 1612 |
| NspI | RCATGY | 5 cut(s) | 1550, 1734, 1963, 1992, 2098 |
| PaeI | GCATGC | 1 cut(s) | 1963 |
| PagI | TCATGA | 1 cut(s) | 1246 |
| PaqCI | CACCTGC | 1 cut(s) | 941 |
| PciI | ACATGT | 2 cut(s) | 1988, 2094 |
| PfeI | GAWTC | 2 cut(s) | 712, 1317 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 938 |
| PkrI | GCNGC | 4 cut(s) | 909, 1494, 1545, 1918 |
| PleI | GAGTC | 2 cut(s) | 1637, 2165 |
| PmeI | GTTTAAAC | 1 cut(s) | 508 |
| PpsI | GAGTC | 2 cut(s) | 1637, 2165 |
| Ppu21I | YACGTR | 1 cut(s) | 1791 |
| PscI | ACATGT | 2 cut(s) | 1988, 2094 |
| PshBI | ATTAAT | 1 cut(s) | 1817 |
| Psp6I | CCWGG | 1 cut(s) | 1831 |
| PspFI | CCCAGC | 2 cut(s) | 1000, 1942 |
| PspGI | CCWGG | 1 cut(s) | 1831 |
| PspN4I | GGNNCC | 3 cut(s) | 1404, 1765, 2382 |
| PspPI | GGNCC | 4 cut(s) | 446, 749, 1240, 1835 |
| PstNI | CAGNNNCTG | 2 cut(s) | 44, 907 |
| PsuI | RGATCY | 3 cut(s) | 55, 1456, 2380 |
| RsaI | GTAC | 8 cut(s) | 480, 531, 814, 900, 1383, 1765, 2291, 2365 |
| RsaNI | GTAC | 8 cut(s) | 479, 530, 813, 899, 1382, 1764, 2290, 2364 |
| RseI | CAYNNNNRTG | 3 cut(s) | 222, 1533, 2186 |
| SalI | GTCGAC | 1 cut(s) | 1992 |
| SaqAI | TTAA | 7 cut(s) | 119, 507, 702, 1350, 1770, 1817, 2196 |
| SatI | GCNGC | 4 cut(s) | 908, 1493, 1544, 1917 |
| Sau96I | GGNCC | 4 cut(s) | 446, 749, 1240, 1835 |
| SchI | GAGTC | 2 cut(s) | 1637, 2165 |
| ScrFI | CCNGG | 2 cut(s) | 881, 1833 |
| SduI | GDGCHC | 8 cut(s) | 253, 739, 824, 868, 1796, 2071, 2115, 2131 |
| SfaNI | GCATC | 7 cut(s) | 328, 415, 937, 1000, 1318, 1340, 1597 |
| SfcI | CTRYAG | 1 cut(s) | 333 |
| SinI | GGWCC | 1 cut(s) | 1240 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 222, 1533, 2186 |
| SmlI | CTYRAG | 1 cut(s) | 789 |
| SmoI | CTYRAG | 1 cut(s) | 789 |
| SpeI | ACTAGT | 1 cut(s) | 2275 |
| SphI | GCATGC | 1 cut(s) | 1963 |
| SsiI | CCGC | 4 cut(s) | 809, 1477, 2155, 2359 |
| SspMI | CTAG | 4 cut(s) | 569, 740, 2054, 2276 |
| StyD4I | CCNGG | 2 cut(s) | 879, 1831 |
| StyI | CCWWGG | 6 cut(s) | 291, 466, 1169, 2011, 2122, 2214 |
| TaaI | ACNGT | 8 cut(s) | 40, 709, 920, 1057, 1560, 1577, 1683, 1724 |
| TaiI | ACGT | 4 cut(s) | 506, 600, 1138, 1793 |
| TaqI | TCGA | 6 cut(s) | 343, 423, 600, 1899, 1993, 2174 |
| TatI | WGTACW | 2 cut(s) | 478, 898 |
| TfiI | GAWTC | 2 cut(s) | 712, 1317 |
| Tru1I | TTAA | 7 cut(s) | 119, 507, 702, 1350, 1770, 1817, 2196 |
| Tru9I | TTAA | 7 cut(s) | 119, 507, 702, 1350, 1770, 1817, 2196 |
| TscAI | CASTG | 3 cut(s) | 49, 714, 919 |
| TseFI | GTSAC | 3 cut(s) | 40, 652, 2168 |
| TseI | GCWGC | 4 cut(s) | 907, 1492, 1543, 1916 |
| Tsp45I | GTSAC | 3 cut(s) | 40, 652, 2168 |
| TspDTI | ATGAA | 9 cut(s) | 240, 573, 1036, 1235, 1334, 1703, 1920, 1989, 2300 |
| TspGWI | ACGGA | 4 cut(s) | 253, 1340, 2307, 2387 |
| TspRI | CASTG | 3 cut(s) | 49, 714, 919 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 938 |
| VneI | GTGCAC | 2 cut(s) | 249, 735 |
| VpaK11BI | GGWCC | 1 cut(s) | 1240 |
| VspI | ATTAAT | 1 cut(s) | 1817 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1247 |
| XapI | RAATTY | 2 cut(s) | 518, 1094 |
| XbaI | TCTAGA | 1 cut(s) | 2053 |
| XceI | RCATGY | 5 cut(s) | 1550, 1734, 1963, 1992, 2098 |
| XmiI | GTMKAC | 3 cut(s) | 785, 1678, 1993 |
| XspI | CTAG | 4 cut(s) | 569, 740, 2054, 2276 |
| ZraI | GACGTC | 1 cut(s) | 598 |
| Zsp2I | ATGCAT | 2 cut(s) | 1333, 1612 |