Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G32000 AT2G32000 |
| fragaria_vesca | FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 |
| malus_domestica | MD04G1023600.v1.1 |
| prunus_persica | Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 |
| pyrus_communis | pycom04g02020 |
| rosa_chinensis | RchiOBHm_Chr4g0412031 |
| rosa_laevigata | RLG00000008327 |
| rosa_multiflora | Rmu_co8041726.1_g000001 Rmu_sc0000711.1_g000051 |
| rosa_roxburghii | Rroxscaffold_1G00042480 Rroxscaffold_5G00346360 Rroxscaffold_5G00356250 Rroxscaffold_5G00356340 |
| rosa_rugosa | Rorug04G0106700 Rorug04G0106800 Rorug04G0106900 |
| rosa_samantha | Rh4AG171100 Rh4BG168800 Rh4BG169700 Rh4CG181900 Rh4DG164200 Rh4DG165300 |
| rosa_wichuraiana | Rw0G007770 Rw0G008800 Rw4G014260 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1172 |
| Acc36I | ACCTGC | 3 cut(s) | 1172, 1600, 1813 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1169 |
| AccI | GTMKAC | 2 cut(s) | 149, 1016 |
| AccII | CGCG | 1 cut(s) | 1042 |
| AciI | CCGC | 2 cut(s) | 1040, 1708 |
| AclI | AACGTT | 1 cut(s) | 63 |
| AclWI | GGATC | 3 cut(s) | 284, 306, 1676 |
| AcoI | YGGCCR | 1 cut(s) | 496 |
| AcsI | RAATTY | 3 cut(s) | 179, 749, 1325 |
| AcuI | CTGAAG | 1 cut(s) | 779 |
| AdeI | CACNNNGTG | 1 cut(s) | 1094 |
| AfaI | GTAC | 6 cut(s) | 174, 210, 762, 1045, 1131, 1614 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 988, 1117, 1118, 1169, 1480 |
| AflIII | ACRYGT | 1 cut(s) | 1366 |
| AjnI | CCWGG | 1 cut(s) | 742 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1317, 1349 |
| AluBI | AGCT | 7 cut(s) | 47, 311, 374, 799, 1035, 1522, 1619 |
| AluI | AGCT | 7 cut(s) | 47, 311, 374, 799, 1035, 1522, 1619 |
| Alw21I | GWGCWC | 4 cut(s) | 535, 970, 1055, 1099 |
| Alw44I | GTGCAC | 3 cut(s) | 531, 966, 1095 |
| AlwI | GGATC | 3 cut(s) | 284, 306, 1676 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 287, 1138 |
| Ama87I | CYCGRG | 1 cut(s) | 375 |
| AoxI | GGCC | 4 cut(s) | 159, 496, 570, 979 |
| ApaLI | GTGCAC | 3 cut(s) | 531, 966, 1095 |
| ApeKI | GCWGC | 3 cut(s) | 1138, 1723, 1774 |
| ApoI | RAATTY | 3 cut(s) | 179, 749, 1325 |
| AspLEI | GCGC | 1 cut(s) | 1599 |
| AspS9I | GGNCC | 2 cut(s) | 980, 1471 |
| AsuC2I | CCSGG | 1 cut(s) | 1112 |
| AsuHPI | GGTGA | 3 cut(s) | 1196, 1387, 1485 |
| AvaI | CYCGRG | 1 cut(s) | 375 |
| AvaII | GGWCC | 1 cut(s) | 1471 |
| AxyI | CCTNAGG | 1 cut(s) | 1645 |
| BaeGI | GKGCMC | 3 cut(s) | 535, 970, 1099 |
| BalI | TGGCCA | 1 cut(s) | 498 |
| Bbv12I | GWGCWC | 4 cut(s) | 535, 970, 1055, 1099 |
| BbvI | GCAGC | 3 cut(s) | 1125, 1735, 1786 |
| BccI | CCATC | 1 cut(s) | 863 |
| BciT130I | CCWGG | 1 cut(s) | 744 |
| BclI | TGATCA | 1 cut(s) | 1186 |
| BcnI | CCSGG | 1 cut(s) | 1112 |
| BfaI | CTAG | 3 cut(s) | 251, 800, 971 |
| BfmI | CTRYAG | 2 cut(s) | 140, 615 |
| BfuAI | ACCTGC | 3 cut(s) | 1172, 1600, 1813 |
| BglII | AGATCT | 1 cut(s) | 1687 |
| BisI | GCNGC | 3 cut(s) | 1139, 1724, 1775 |
| BlsI | GCNGC | 3 cut(s) | 1140, 1725, 1776 |
| Bme1390I | CCNGG | 2 cut(s) | 744, 1112 |
| Bme18I | GGWCC | 1 cut(s) | 1471 |
| BmeT110I | CYCGRG | 1 cut(s) | 375 |
| BmgT120I | GGNCC | 2 cut(s) | 980, 1471 |
| BmiI | GGNNCC | 1 cut(s) | 1635 |
| BmrFI | CCNGG | 2 cut(s) | 744, 1112 |
| BmrI | ACTGGG | 2 cut(s) | 33, 814 |
| BmsI | GCATC | 6 cut(s) | 610, 697, 1168, 1231, 1549, 1571 |
| BmuI | ACTGGG | 2 cut(s) | 33, 814 |
| BpmI | CTGGAG | 2 cut(s) | 297, 1482 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1743 |
| BpuEI | CTTGAG | 1 cut(s) | 1005 |
| BpuMI | CCSGG | 1 cut(s) | 1112 |
| BsaBI | GATNNNNATC | 2 cut(s) | 411, 1686 |
| BsaJI | CCNNGG | 2 cut(s) | 573, 1400 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1276, 1306, 1480, 1510 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 988, 1117, 1118, 1169, 1480 |
| Bse1I | ACTGG | 4 cut(s) | 39, 281, 314, 809 |
| Bse21I | CCTNAGG | 1 cut(s) | 1645 |
| Bse8I | GATNNNNATC | 2 cut(s) | 411, 1686 |
| BseBI | CCWGG | 1 cut(s) | 744 |
| BseDI | CCNNGG | 2 cut(s) | 573, 1400 |
| BseGI | GGATG | 2 cut(s) | 1189, 1564 |
| BseJI | GATNNNNATC | 2 cut(s) | 411, 1686 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 988, 1117, 1118, 1169, 1480 |
| BseMII | CTCAG | 1 cut(s) | 1212 |
| BseNI | ACTGG | 4 cut(s) | 39, 281, 314, 809 |
| BseSI | GKGCMC | 3 cut(s) | 535, 970, 1099 |
| BseXI | GCAGC | 3 cut(s) | 1125, 1735, 1786 |
| BseYI | CCCAGC | 1 cut(s) | 1231 |
| BsgI | GTGCAG | 2 cut(s) | 1628, 1793 |
| Bsh1236I | CGCG | 1 cut(s) | 1042 |
| BshFI | GGCC | 4 cut(s) | 161, 498, 572, 981 |
| BsiHKAI | GWGCWC | 4 cut(s) | 535, 970, 1055, 1099 |
| BsiHKCI | CYCGRG | 1 cut(s) | 375 |
| BsiSI | CCGG | 1 cut(s) | 1112 |
| BslFI | GGGAC | 2 cut(s) | 1186, 1297 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 988, 1117, 1118, 1169, 1480 |
| BsmFI | GGGAC | 2 cut(s) | 1186, 1297 |
| BsnI | GGCC | 4 cut(s) | 161, 498, 572, 981 |
| BsoBI | CYCGRG | 1 cut(s) | 375 |
| Bsp1286I | GDGCHC | 4 cut(s) | 535, 970, 1055, 1099 |
| Bsp1407I | TGTACA | 2 cut(s) | 208, 1129 |
| Bsp143I | GATC | 8 cut(s) | 289, 298, 412, 1166, 1186, 1681, 1687, 1756 |
| Bsp19I | CCATGG | 2 cut(s) | 573, 1400 |
| BspACI | CCGC | 2 cut(s) | 1040, 1708 |
| BspANI | GGCC | 4 cut(s) | 161, 498, 572, 981 |
| BspCNI | CTCAG | 1 cut(s) | 1213 |
| BspFNI | CGCG | 1 cut(s) | 1042 |
| BspHI | TCATGA | 2 cut(s) | 81, 1477 |
| BspLI | GGNNCC | 1 cut(s) | 1635 |
| BspMI | ACCTGC | 3 cut(s) | 1172, 1600, 1813 |
| BspPI | GGATC | 3 cut(s) | 284, 306, 1676 |
| BsrGI | TGTACA | 2 cut(s) | 208, 1129 |
| BsrI | ACTGG | 4 cut(s) | 39, 281, 314, 809 |
| BssECI | CCNNGG | 2 cut(s) | 573, 1400 |
| BssMI | GATC | 8 cut(s) | 289, 298, 412, 1166, 1186, 1681, 1687, 1756 |
| BssNAI | GTATAC | 1 cut(s) | 1017 |
| BssT1I | CCWWGG | 2 cut(s) | 573, 1400 |
| Bst1107I | GTATAC | 1 cut(s) | 1017 |
| Bst2UI | CCWGG | 1 cut(s) | 744 |
| Bst4CI | ACNGT | 7 cut(s) | 213, 283, 940, 1151, 1288, 1791, 1808 |
| BstAUI | TGTACA | 2 cut(s) | 208, 1129 |
| BstC8I | GCNNGC | 3 cut(s) | 163, 356, 1621 |
| BstDEI | CTNAG | 4 cut(s) | 1221, 1508, 1645, 1743 |
| BstDSI | CCRYGG | 2 cut(s) | 573, 1400 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1478 |
| BstF5I | GGATG | 2 cut(s) | 1189, 1564 |
| BstFNI | CGCG | 1 cut(s) | 1042 |
| BstHHI | GCGC | 1 cut(s) | 1599 |
| BstKTI | GATC | 8 cut(s) | 292, 301, 415, 1169, 1189, 1684, 1690, 1759 |
| BstMBI | GATC | 8 cut(s) | 289, 298, 412, 1166, 1186, 1681, 1687, 1756 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 620, 965, 1032, 1528 |
| BstNI | CCWGG | 1 cut(s) | 744 |
| BstNSI | RCATGY | 2 cut(s) | 242, 1781 |
| BstSCI | CCNGG | 2 cut(s) | 742, 1110 |
| BstSFI | CTRYAG | 2 cut(s) | 140, 615 |
| BstSLI | GKGCMC | 3 cut(s) | 535, 970, 1099 |
| BstUI | CGCG | 1 cut(s) | 1042 |
| BstV1I | GCAGC | 3 cut(s) | 1125, 1735, 1786 |
| BstX2I | RGATCY | 2 cut(s) | 298, 1687 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 67 |
| BstYI | RGATCY | 2 cut(s) | 298, 1687 |
| BstZ17I | GTATAC | 1 cut(s) | 1017 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1645 |
| BsuRI | GGCC | 4 cut(s) | 161, 498, 572, 981 |
| BtgI | CCRYGG | 2 cut(s) | 573, 1400 |
| BtsCI | GGATG | 2 cut(s) | 1189, 1564 |
| BtsIMutI | CAGTG | 3 cut(s) | 285, 945, 1143 |
| BveI | ACCTGC | 3 cut(s) | 1172, 1600, 1813 |
| Cac8I | GCNNGC | 3 cut(s) | 163, 356, 1621 |
| CaiI | CAGNNNCTG | 2 cut(s) | 287, 1138 |
| CciI | TCATGA | 2 cut(s) | 81, 1477 |
| CfoI | GCGC | 1 cut(s) | 1599 |
| Cfr13I | GGNCC | 2 cut(s) | 980, 1471 |
| Csp6I | GTAC | 6 cut(s) | 173, 209, 761, 1044, 1130, 1613 |
| CviQI | GTAC | 6 cut(s) | 173, 209, 761, 1044, 1130, 1613 |
| DdeI | CTNAG | 4 cut(s) | 1221, 1508, 1645, 1743 |
| DpnI | GATC | 8 cut(s) | 291, 300, 414, 1168, 1188, 1683, 1689, 1758 |
| DpnII | GATC | 8 cut(s) | 289, 298, 412, 1166, 1186, 1681, 1687, 1756 |
| DraI | TTTAAA | 2 cut(s) | 217, 739 |
| DraIII | CACNNNGTG | 1 cut(s) | 1094 |
| EaeI | YGGCCR | 1 cut(s) | 496 |
| Eco130I | CCWWGG | 2 cut(s) | 573, 1400 |
| Eco32I | GATATC | 1 cut(s) | 1705 |
| Eco47I | GGWCC | 1 cut(s) | 1471 |
| Eco57I | CTGAAG | 1 cut(s) | 779 |
| Eco81I | CCTNAGG | 1 cut(s) | 1645 |
| Eco88I | CYCGRG | 1 cut(s) | 375 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1478 |
| EcoRII | CCWGG | 1 cut(s) | 742 |
| EcoRV | GATATC | 1 cut(s) | 1705 |
| EcoT14I | CCWWGG | 2 cut(s) | 573, 1400 |
| EcoT22I | ATGCAT | 1 cut(s) | 1564 |
| ErhI | CCWWGG | 2 cut(s) | 573, 1400 |
| FaqI | GGGAC | 2 cut(s) | 1186, 1297 |
| FauNDI | CATATG | 1 cut(s) | 1589 |
| FbaI | TGATCA | 1 cut(s) | 1186 |
| FblI | GTMKAC | 2 cut(s) | 149, 1016 |
| Fnu4HI | GCNGC | 3 cut(s) | 1139, 1724, 1775 |
| FokI | GGATG | 2 cut(s) | 1176, 1571 |
| Fsp4HI | GCNGC | 3 cut(s) | 1139, 1724, 1775 |
| FspBI | CTAG | 3 cut(s) | 251, 800, 971 |
| GlaI | GCGC | 1 cut(s) | 1598 |
| GluI | GCNGC | 3 cut(s) | 1139, 1724, 1775 |
| GsaI | CCCAGC | 1 cut(s) | 1235 |
| GsuI | CTGGAG | 2 cut(s) | 297, 1482 |
| HaeIII | GGCC | 4 cut(s) | 161, 498, 572, 981 |
| HapII | CCGG | 1 cut(s) | 1112 |
| HhaI | GCGC | 1 cut(s) | 1599 |
| Hin6I | GCGC | 1 cut(s) | 1597 |
| HinP1I | GCGC | 1 cut(s) | 1597 |
| HinfI | GANTC | 5 cut(s) | 78, 85, 201, 943, 1548 |
| HpaII | CCGG | 1 cut(s) | 1112 |
| HphI | GGTGA | 3 cut(s) | 1196, 1387, 1485 |
| Hpy166II | GTNNAC | 7 cut(s) | 150, 533, 968, 1017, 1097, 1271, 1471 |
| Hpy188I | TCNGA | 3 cut(s) | 650, 898, 1796 |
| Hpy8I | GTNNAC | 7 cut(s) | 150, 533, 968, 1017, 1097, 1271, 1471 |
| HpyAV | CCTTC | 6 cut(s) | 413, 562, 933, 1074, 1372, 1484 |
| HpyCH4III | ACNGT | 7 cut(s) | 213, 283, 940, 1151, 1288, 1791, 1808 |
| HpyCH4IV | ACGT | 5 cut(s) | 63, 171, 734, 828, 1366 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 620, 965, 1032, 1528 |
| HpyF3I | CTNAG | 4 cut(s) | 1221, 1508, 1645, 1743 |
| HpySE526I | ACGT | 5 cut(s) | 63, 171, 734, 828, 1366 |
| HspAI | GCGC | 1 cut(s) | 1597 |
| Ksp22I | TGATCA | 1 cut(s) | 1186 |
| Kzo9I | GATC | 8 cut(s) | 289, 298, 412, 1166, 1186, 1681, 1687, 1756 |
| LmnI | GCTCC | 2 cut(s) | 316, 808 |
| Lsp1109I | GCAGC | 3 cut(s) | 1125, 1735, 1786 |
| LweI | GCATC | 6 cut(s) | 610, 697, 1168, 1231, 1549, 1571 |
| MaeI | CTAG | 3 cut(s) | 251, 800, 971 |
| MaeII | ACGT | 5 cut(s) | 63, 171, 734, 828, 1366 |
| MaeIII | GTNAC | 7 cut(s) | 220, 283, 386, 547, 812, 883, 1198 |
| MalI | GATC | 8 cut(s) | 291, 300, 414, 1168, 1188, 1683, 1689, 1758 |
| MboI | GATC | 8 cut(s) | 289, 298, 412, 1166, 1186, 1681, 1687, 1756 |
| MboII | GAAGA | 7 cut(s) | 534, 878, 938, 1059, 1339, 1418, 1552 |
| MfeI | CAATTG | 1 cut(s) | 1332 |
| MflI | RGATCY | 2 cut(s) | 298, 1687 |
| MhlI | GDGCHC | 4 cut(s) | 535, 970, 1055, 1099 |
| MlsI | TGGCCA | 1 cut(s) | 498 |
| MluCI | AATT | 9 cut(s) | 179, 749, 818, 873, 1325, 1332, 1433, 1524, 1600 |
| MluNI | TGGCCA | 1 cut(s) | 498 |
| MmeI | TCCRAC | 4 cut(s) | 33, 628, 1661, 1819 |
| Mox20I | TGGCCA | 1 cut(s) | 498 |
| Mph1103I | ATGCAT | 1 cut(s) | 1564 |
| MscI | TGGCCA | 1 cut(s) | 498 |
| MseI | TTAA | 5 cut(s) | 216, 362, 738, 933, 1581 |
| MslI | CAYNNNNRTG | 2 cut(s) | 504, 1764 |
| Msp20I | TGGCCA | 1 cut(s) | 498 |
| MspI | CCGG | 1 cut(s) | 1112 |
| MspR9I | CCNGG | 2 cut(s) | 744, 1112 |
| MssI | GTTTAAAC | 1 cut(s) | 739 |
| MunI | CAATTG | 1 cut(s) | 1332 |
| MvaI | CCWGG | 1 cut(s) | 744 |
| MvnI | CGCG | 1 cut(s) | 1042 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 620, 965, 1032, 1528 |
| NciI | CCSGG | 1 cut(s) | 1112 |
| NcoI | CCATGG | 2 cut(s) | 573, 1400 |
| NdeI | CATATG | 1 cut(s) | 1589 |
| NdeII | GATC | 8 cut(s) | 289, 298, 412, 1166, 1186, 1681, 1687, 1756 |
| NlaIV | GGNNCC | 1 cut(s) | 1635 |
| NmeAIII | GCCGAG | 1 cut(s) | 55 |
| NmuCI | GTSAC | 3 cut(s) | 220, 283, 883 |
| NsiI | ATGCAT | 1 cut(s) | 1564 |
| NspI | RCATGY | 2 cut(s) | 242, 1781 |
| PagI | TCATGA | 2 cut(s) | 81, 1477 |
| PaqCI | CACCTGC | 1 cut(s) | 1172 |
| PfeI | GAWTC | 5 cut(s) | 78, 85, 201, 943, 1548 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1169 |
| PkrI | GCNGC | 3 cut(s) | 1140, 1725, 1776 |
| PmeI | GTTTAAAC | 1 cut(s) | 739 |
| Psp1406I | AACGTT | 1 cut(s) | 63 |
| Psp6I | CCWGG | 1 cut(s) | 742 |
| PspFI | CCCAGC | 1 cut(s) | 1231 |
| PspGI | CCWGG | 1 cut(s) | 742 |
| PspN4I | GGNNCC | 1 cut(s) | 1635 |
| PspPI | GGNCC | 2 cut(s) | 980, 1471 |
| PstNI | CAGNNNCTG | 2 cut(s) | 287, 1138 |
| PsuI | RGATCY | 2 cut(s) | 298, 1687 |
| RsaI | GTAC | 6 cut(s) | 174, 210, 762, 1045, 1131, 1614 |
| RsaNI | GTAC | 6 cut(s) | 173, 209, 761, 1044, 1130, 1613 |
| RseI | CAYNNNNRTG | 2 cut(s) | 504, 1764 |
| SaqAI | TTAA | 5 cut(s) | 216, 362, 738, 933, 1581 |
| SatI | GCNGC | 3 cut(s) | 1139, 1724, 1775 |
| Sau3AI | GATC | 8 cut(s) | 289, 298, 412, 1166, 1186, 1681, 1687, 1756 |
| Sau96I | GGNCC | 2 cut(s) | 980, 1471 |
| ScrFI | CCNGG | 2 cut(s) | 744, 1112 |
| SduI | GDGCHC | 4 cut(s) | 535, 970, 1055, 1099 |
| SfaNI | GCATC | 6 cut(s) | 610, 697, 1168, 1231, 1549, 1571 |
| SfcI | CTRYAG | 2 cut(s) | 140, 615 |
| SinI | GGWCC | 1 cut(s) | 1471 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 504, 1764 |
| SmlI | CTYRAG | 1 cut(s) | 1020 |
| SmoI | CTYRAG | 1 cut(s) | 1020 |
| Sse9I | AATT | 9 cut(s) | 179, 749, 818, 873, 1325, 1332, 1433, 1524, 1600 |
| SsiI | CCGC | 2 cut(s) | 1040, 1708 |
| SspMI | CTAG | 3 cut(s) | 251, 800, 971 |
| StyD4I | CCNGG | 2 cut(s) | 742, 1110 |
| StyI | CCWWGG | 2 cut(s) | 573, 1400 |
| TaaI | ACNGT | 7 cut(s) | 213, 283, 940, 1151, 1288, 1791, 1808 |
| TaiI | ACGT | 5 cut(s) | 66, 174, 737, 831, 1369 |
| TaqI | TCGA | 2 cut(s) | 625, 705 |
| TasI | AATT | 9 cut(s) | 179, 749, 818, 873, 1325, 1332, 1433, 1524, 1600 |
| TatI | WGTACW | 2 cut(s) | 208, 1129 |
| TfiI | GAWTC | 5 cut(s) | 78, 85, 201, 943, 1548 |
| Tru1I | TTAA | 5 cut(s) | 216, 362, 738, 933, 1581 |
| Tru9I | TTAA | 5 cut(s) | 216, 362, 738, 933, 1581 |
| TscAI | CASTG | 3 cut(s) | 292, 945, 1150 |
| TseFI | GTSAC | 3 cut(s) | 220, 283, 883 |
| TseI | GCWGC | 3 cut(s) | 1138, 1723, 1774 |
| Tsp45I | GTSAC | 3 cut(s) | 220, 283, 883 |
| TspGWI | ACGGA | 2 cut(s) | 182, 1571 |
| TspRI | CASTG | 3 cut(s) | 292, 945, 1150 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1169 |
| VneI | GTGCAC | 3 cut(s) | 531, 966, 1095 |
| VpaK11BI | GGWCC | 1 cut(s) | 1471 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1478 |
| XapI | RAATTY | 3 cut(s) | 179, 749, 1325 |
| XbaI | TCTAGA | 1 cut(s) | 250 |
| XceI | RCATGY | 2 cut(s) | 242, 1781 |
| XmiI | GTMKAC | 2 cut(s) | 149, 1016 |
| XspI | CTAG | 3 cut(s) | 251, 800, 971 |
| Zsp2I | ATGCAT | 1 cut(s) | 1564 |