Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G32000 AT2G32000 |
| fragaria_vesca | FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 |
| malus_domestica | MD04G1023600.v1.1 |
| prunus_persica | Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 |
| pyrus_communis | pycom04g02020 |
| rosa_chinensis | RchiOBHm_Chr4g0412031 |
| rosa_laevigata | RLG00000008327 |
| rosa_multiflora | Rmu_co8041726.1_g000001 Rmu_sc0000711.1_g000051 |
| rosa_roxburghii | Rroxscaffold_1G00042480 Rroxscaffold_5G00346360 Rroxscaffold_5G00356250 Rroxscaffold_5G00356340 |
| rosa_rugosa | Rorug04G0106700 Rorug04G0106800 Rorug04G0106900 |
| rosa_samantha | Rh4AG171100 Rh4BG168800 Rh4BG169700 Rh4CG181900 Rh4DG164200 Rh4DG165300 |
| rosa_wichuraiana | Rw0G007770 Rw0G008800 Rw4G014260 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 650 |
| AatII | GACGTC | 1 cut(s) | 309 |
| Acc36I | ACCTGC | 2 cut(s) | 650, 1078 |
| Acc65I | GGTACC | 1 cut(s) | 1472 |
| AccB1I | GGYRCC | 1 cut(s) | 1472 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 647 |
| AccBSI | CCGCTC | 1 cut(s) | 1864 |
| AccI | GTMKAC | 2 cut(s) | 494, 1702 |
| AccII | CGCG | 1 cut(s) | 520 |
| AciI | CCGC | 4 cut(s) | 518, 1186, 1864, 2068 |
| AclWI | GGATC | 3 cut(s) | 1154, 2050, 2084 |
| AcsI | RAATTY | 2 cut(s) | 227, 803 |
| AcyI | GRCGYC | 1 cut(s) | 306 |
| AfaI | GTAC | 8 cut(s) | 189, 240, 523, 609, 1092, 1474, 2000, 2074 |
| AfeI | AGCGCT | 1 cut(s) | 194 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 176, 595, 596, 647, 958, 1469, 1601, 1834 |
| AflIII | ACRYGT | 3 cut(s) | 844, 1697, 1803 |
| AhlI | ACTAGT | 1 cut(s) | 1984 |
| AjnI | CCWGG | 1 cut(s) | 1540 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 795, 827 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1743, 1775 |
| Alw21I | GWGCWC | 5 cut(s) | 448, 533, 577, 1780, 1824 |
| Alw26I | GTCTC | 1 cut(s) | 1430 |
| Alw44I | GTGCAC | 2 cut(s) | 444, 573 |
| AlwI | GGATC | 3 cut(s) | 1154, 2050, 2084 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 616 |
| Aor51HI | AGCGCT | 1 cut(s) | 194 |
| AoxI | GGCC | 2 cut(s) | 155, 1543 |
| ApaLI | GTGCAC | 2 cut(s) | 444, 573 |
| ApeKI | GCWGC | 4 cut(s) | 616, 1201, 1252, 1625 |
| ApoI | RAATTY | 2 cut(s) | 227, 803 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1601, 1633 |
| AseI | ATTAAT | 1 cut(s) | 1526 |
| Asp718I | GGTACC | 1 cut(s) | 1472 |
| AspLEI | GCGC | 2 cut(s) | 195, 1077 |
| AspS9I | GGNCC | 4 cut(s) | 155, 458, 949, 1544 |
| AsuC2I | CCSGG | 1 cut(s) | 590 |
| AsuHPI | GGTGA | 3 cut(s) | 674, 865, 963 |
| AvaII | GGWCC | 2 cut(s) | 458, 949 |
| BaeGI | GKGCMC | 3 cut(s) | 448, 577, 1505 |
| BanI | GGYRCC | 1 cut(s) | 1472 |
| BanII | GRGCYC | 1 cut(s) | 1840 |
| BbsI | GAAGAC | 1 cut(s) | 351 |
| Bbv12I | GWGCWC | 5 cut(s) | 448, 533, 577, 1780, 1824 |
| BbvI | GCAGC | 4 cut(s) | 603, 1213, 1264, 1612 |
| BccI | CCATC | 2 cut(s) | 1544, 1925 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1779, 1813 |
| BciT130I | CCWGG | 1 cut(s) | 1542 |
| BclI | TGATCA | 1 cut(s) | 664 |
| BcnI | CCSGG | 1 cut(s) | 590 |
| BcoDI | GTCTC | 1 cut(s) | 1430 |
| BcuI | ACTAGT | 1 cut(s) | 1984 |
| BfaI | CTAG | 4 cut(s) | 278, 449, 1763, 1985 |
| BfmI | CTRYAG | 1 cut(s) | 42 |
| BfoI | RGCGCY | 1 cut(s) | 196 |
| BfuAI | ACCTGC | 2 cut(s) | 650, 1078 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1551 |
| BglII | AGATCT | 1 cut(s) | 1165 |
| BisI | GCNGC | 4 cut(s) | 617, 1202, 1253, 1626 |
| BlsI | GCNGC | 4 cut(s) | 618, 1203, 1254, 1627 |
| Bme1390I | CCNGG | 2 cut(s) | 590, 1542 |
| Bme18I | GGWCC | 2 cut(s) | 458, 949 |
| BmgT120I | GGNCC | 4 cut(s) | 155, 458, 949, 1544 |
| BmiI | GGNNCC | 2 cut(s) | 1113, 1474 |
| BmrFI | CCNGG | 2 cut(s) | 590, 1542 |
| BmrI | ACTGGG | 2 cut(s) | 292, 2085 |
| BmsI | GCATC | 8 cut(s) | 37, 124, 646, 696, 709, 1027, 1049, 1306 |
| BmuI | ACTGGG | 2 cut(s) | 292, 2085 |
| BpiI | GAAGAC | 1 cut(s) | 351 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1865, 1897 |
| BpmI | CTGGAG | 3 cut(s) | 257, 960, 1842 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1221 |
| BpuEI | CTTGAG | 1 cut(s) | 483 |
| BpuMI | CCSGG | 1 cut(s) | 590 |
| BsaAI | YACGTR | 1 cut(s) | 1500 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1164 |
| BsaHI | GRCGYC | 1 cut(s) | 306 |
| BsaJI | CCNNGG | 6 cut(s) | 175, 878, 1122, 1720, 1831, 1923 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 754, 784, 958, 988, 1982, 2012 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 176, 595, 596, 647, 958, 1469, 1601, 1834 |
| Bse1I | ACTGG | 3 cut(s) | 287, 1954, 2080 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1164 |
| BseBI | CCWGG | 1 cut(s) | 1542 |
| BseDI | CCNNGG | 6 cut(s) | 175, 878, 1122, 1720, 1831, 1923 |
| BseGI | GGATG | 4 cut(s) | 667, 1042, 1321, 1690 |
| BseJI | GATNNNNATC | 1 cut(s) | 1164 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 176, 595, 596, 647, 958, 1469, 1601, 1834 |
| BseMII | CTCAG | 5 cut(s) | 690, 1368, 1381, 1478, 1863 |
| BseNI | ACTGG | 3 cut(s) | 287, 1954, 2080 |
| BseRI | GAGGAG | 2 cut(s) | 716, 1814 |
| BseSI | GKGCMC | 3 cut(s) | 448, 577, 1505 |
| BseXI | GCAGC | 4 cut(s) | 603, 1213, 1264, 1612 |
| BseYI | CCCAGC | 1 cut(s) | 1651 |
| BsgI | GTGCAG | 2 cut(s) | 1106, 1271 |
| Bsh1236I | CGCG | 1 cut(s) | 520 |
| BshFI | GGCC | 2 cut(s) | 157, 1545 |
| BshNI | GGYRCC | 1 cut(s) | 1472 |
| BsiHKAI | GWGCWC | 5 cut(s) | 448, 533, 577, 1780, 1824 |
| BsiSI | CCGG | 1 cut(s) | 590 |
| BslFI | GGGAC | 2 cut(s) | 664, 775 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 176, 595, 596, 647, 958, 1469, 1601, 1834 |
| BsmAI | GTCTC | 1 cut(s) | 1430 |
| BsmBI | CGTCTC | 1 cut(s) | 1430 |
| BsmFI | GGGAC | 2 cut(s) | 664, 775 |
| BsnI | GGCC | 2 cut(s) | 157, 1545 |
| Bsp1286I | GDGCHC | 7 cut(s) | 448, 533, 577, 1505, 1780, 1824, 1840 |
| Bsp1407I | TGTACA | 2 cut(s) | 187, 607 |
| Bsp143I | GATC | 7 cut(s) | 644, 664, 1159, 1165, 1234, 2042, 2089 |
| Bsp19I | CCATGG | 1 cut(s) | 878 |
| BspACI | CCGC | 4 cut(s) | 518, 1186, 1864, 2068 |
| BspANI | GGCC | 2 cut(s) | 157, 1545 |
| BspCNI | CTCAG | 5 cut(s) | 691, 1367, 1380, 1477, 1864 |
| BspFNI | CGCG | 1 cut(s) | 520 |
| BspHI | TCATGA | 1 cut(s) | 955 |
| BspLI | GGNNCC | 2 cut(s) | 1113, 1474 |
| BspMI | ACCTGC | 2 cut(s) | 650, 1078 |
| BspPI | GGATC | 3 cut(s) | 1154, 2050, 2084 |
| BspT107I | GGYRCC | 1 cut(s) | 1472 |
| BsrBI | CCGCTC | 1 cut(s) | 1864 |
| BsrGI | TGTACA | 2 cut(s) | 187, 607 |
| BsrI | ACTGG | 3 cut(s) | 287, 1954, 2080 |
| BssECI | CCNNGG | 6 cut(s) | 175, 878, 1122, 1720, 1831, 1923 |
| BssMI | GATC | 7 cut(s) | 644, 664, 1159, 1165, 1234, 2042, 2089 |
| BssNAI | GTATAC | 1 cut(s) | 495 |
| BssNI | GRCGYC | 1 cut(s) | 306 |
| BssT1I | CCWWGG | 6 cut(s) | 175, 878, 1122, 1720, 1831, 1923 |
| Bst1107I | GTATAC | 1 cut(s) | 495 |
| Bst2UI | CCWGG | 1 cut(s) | 1542 |
| Bst4CI | ACNGT | 7 cut(s) | 418, 629, 766, 1269, 1286, 1392, 1433 |
| Bst6I | CTCTTC | 2 cut(s) | 1440, 2056 |
| BstACI | GRCGYC | 1 cut(s) | 306 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 1674, 1806 |
| BstAUI | TGTACA | 2 cut(s) | 187, 607 |
| BstBAI | YACGTR | 1 cut(s) | 1500 |
| BstC8I | GCNNGC | 6 cut(s) | 566, 1099, 1649, 1666, 1670, 1735 |
| BstDEI | CTNAG | 7 cut(s) | 699, 986, 1221, 1354, 1367, 1464, 1872 |
| BstDSI | CCRYGG | 1 cut(s) | 878 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 956 |
| BstF5I | GGATG | 4 cut(s) | 667, 1042, 1321, 1690 |
| BstFNI | CGCG | 1 cut(s) | 520 |
| BstH2I | RGCGCY | 1 cut(s) | 196 |
| BstHHI | GCGC | 2 cut(s) | 195, 1077 |
| BstKTI | GATC | 7 cut(s) | 647, 667, 1162, 1168, 1237, 2045, 2092 |
| BstMAI | GTCTC | 1 cut(s) | 1430 |
| BstMBI | GATC | 7 cut(s) | 644, 664, 1159, 1165, 1234, 2042, 2089 |
| BstNI | CCWGG | 1 cut(s) | 1542 |
| BstNSI | RCATGY | 5 cut(s) | 1259, 1443, 1672, 1701, 1807 |
| BstSCI | CCNGG | 2 cut(s) | 588, 1540 |
| BstSFI | CTRYAG | 1 cut(s) | 42 |
| BstSLI | GKGCMC | 3 cut(s) | 448, 577, 1505 |
| BstUI | CGCG | 1 cut(s) | 520 |
| BstV1I | GCAGC | 4 cut(s) | 603, 1213, 1264, 1612 |
| BstV2I | GAAGAC | 1 cut(s) | 351 |
| BstX2I | RGATCY | 1 cut(s) | 1165 |
| BstYI | RGATCY | 1 cut(s) | 1165 |
| BstZ17I | GTATAC | 1 cut(s) | 495 |
| BsuRI | GGCC | 2 cut(s) | 157, 1545 |
| BtgI | CCRYGG | 1 cut(s) | 878 |
| BtsCI | GGATG | 4 cut(s) | 667, 1042, 1321, 1690 |
| BtsIMutI | CAGTG | 2 cut(s) | 423, 621 |
| BveI | ACCTGC | 2 cut(s) | 650, 1078 |
| Cac8I | GCNNGC | 6 cut(s) | 566, 1099, 1649, 1666, 1670, 1735 |
| CaiI | CAGNNNCTG | 1 cut(s) | 616 |
| CciI | TCATGA | 1 cut(s) | 955 |
| CfoI | GCGC | 2 cut(s) | 195, 1077 |
| Cfr13I | GGNCC | 4 cut(s) | 155, 458, 949, 1544 |
| Csp6I | GTAC | 8 cut(s) | 188, 239, 522, 608, 1091, 1473, 1999, 2073 |
| CviQI | GTAC | 8 cut(s) | 188, 239, 522, 608, 1091, 1473, 1999, 2073 |
| DdeI | CTNAG | 7 cut(s) | 699, 986, 1221, 1354, 1367, 1464, 1872 |
| DpnI | GATC | 7 cut(s) | 646, 666, 1161, 1167, 1236, 2044, 2091 |
| DpnII | GATC | 7 cut(s) | 644, 664, 1159, 1165, 1234, 2042, 2089 |
| DraI | TTTAAA | 1 cut(s) | 217 |
| Eam1104I | CTCTTC | 2 cut(s) | 1440, 2056 |
| EarI | CTCTTC | 2 cut(s) | 1440, 2056 |
| Eco130I | CCWWGG | 6 cut(s) | 175, 878, 1122, 1720, 1831, 1923 |
| Eco24I | GRGCYC | 1 cut(s) | 1840 |
| Eco32I | GATATC | 2 cut(s) | 1183, 1716 |
| Eco47I | GGWCC | 2 cut(s) | 458, 949 |
| Eco47III | AGCGCT | 1 cut(s) | 194 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 956 |
| EcoRII | CCWGG | 1 cut(s) | 1540 |
| EcoRV | GATATC | 2 cut(s) | 1183, 1716 |
| EcoT14I | CCWWGG | 6 cut(s) | 175, 878, 1122, 1720, 1831, 1923 |
| EcoT22I | ATGCAT | 2 cut(s) | 1042, 1321 |
| EcoT38I | GRGCYC | 1 cut(s) | 1840 |
| ErhI | CCWWGG | 6 cut(s) | 175, 878, 1122, 1720, 1831, 1923 |
| Esp3I | CGTCTC | 1 cut(s) | 1430 |
| FaqI | GGGAC | 2 cut(s) | 664, 775 |
| FauI | CCCGC | 1 cut(s) | 2061 |
| FauNDI | CATATG | 1 cut(s) | 1067 |
| FbaI | TGATCA | 1 cut(s) | 664 |
| FblI | GTMKAC | 2 cut(s) | 494, 1702 |
| Fnu4HI | GCNGC | 4 cut(s) | 617, 1202, 1253, 1626 |
| FokI | GGATG | 4 cut(s) | 654, 1049, 1328, 1677 |
| FriOI | GRGCYC | 1 cut(s) | 1840 |
| Fsp4HI | GCNGC | 4 cut(s) | 617, 1202, 1253, 1626 |
| FspBI | CTAG | 4 cut(s) | 278, 449, 1763, 1985 |
| GlaI | GCGC | 2 cut(s) | 194, 1076 |
| GluI | GCNGC | 4 cut(s) | 617, 1202, 1253, 1626 |
| GsaI | CCCAGC | 1 cut(s) | 1655 |
| GsuI | CTGGAG | 3 cut(s) | 257, 960, 1842 |
| HaeII | RGCGCY | 1 cut(s) | 196 |
| HaeIII | GGCC | 2 cut(s) | 157, 1545 |
| HapII | CCGG | 1 cut(s) | 590 |
| HhaI | GCGC | 2 cut(s) | 195, 1077 |
| Hin1I | GRCGYC | 1 cut(s) | 306 |
| Hin6I | GCGC | 2 cut(s) | 193, 1075 |
| HinP1I | GCGC | 2 cut(s) | 193, 1075 |
| HincII | GTYRAC | 1 cut(s) | 1703 |
| HindII | GTYRAC | 1 cut(s) | 1703 |
| HinfI | GANTC | 5 cut(s) | 421, 1026, 1352, 1725, 1880 |
| HpaII | CCGG | 1 cut(s) | 590 |
| HphI | GGTGA | 3 cut(s) | 674, 865, 963 |
| Hpy166II | GTNNAC | 8 cut(s) | 188, 446, 495, 575, 749, 949, 1703, 1900 |
| Hpy188I | TCNGA | 4 cut(s) | 77, 376, 1467, 2018 |
| Hpy8I | GTNNAC | 8 cut(s) | 188, 446, 495, 575, 749, 949, 1703, 1900 |
| Hpy99I | CGWCG | 1 cut(s) | 311 |
| HpyAV | CCTTC | 7 cut(s) | 411, 552, 850, 962, 1428, 1595, 2025 |
| HpyCH4III | ACNGT | 7 cut(s) | 418, 629, 766, 1269, 1286, 1392, 1433 |
| HpyCH4IV | ACGT | 4 cut(s) | 212, 306, 844, 1499 |
| HpyF3I | CTNAG | 7 cut(s) | 699, 986, 1221, 1354, 1367, 1464, 1872 |
| HpySE526I | ACGT | 4 cut(s) | 212, 306, 844, 1499 |
| Hsp92I | GRCGYC | 1 cut(s) | 306 |
| HspAI | GCGC | 2 cut(s) | 193, 1075 |
| KpnI | GGTACC | 1 cut(s) | 1476 |
| Ksp22I | TGATCA | 1 cut(s) | 664 |
| Kzo9I | GATC | 7 cut(s) | 644, 664, 1159, 1165, 1234, 2042, 2089 |
| LmnI | GCTCC | 5 cut(s) | 286, 1538, 1622, 1783, 1827 |
| Lsp1109I | GCAGC | 4 cut(s) | 603, 1213, 1264, 1612 |
| LweI | GCATC | 8 cut(s) | 37, 124, 646, 696, 709, 1027, 1049, 1306 |
| MaeI | CTAG | 4 cut(s) | 278, 449, 1763, 1985 |
| MaeII | ACGT | 4 cut(s) | 212, 306, 844, 1499 |
| MaeIII | GTNAC | 5 cut(s) | 290, 361, 676, 1877, 1971 |
| MalI | GATC | 7 cut(s) | 646, 666, 1161, 1167, 1236, 2044, 2091 |
| MbiI | CCGCTC | 1 cut(s) | 1864 |
| MboI | GATC | 7 cut(s) | 644, 664, 1159, 1165, 1234, 2042, 2089 |
| MfeI | CAATTG | 1 cut(s) | 810 |
| MflI | RGATCY | 1 cut(s) | 1165 |
| MhlI | GDGCHC | 7 cut(s) | 448, 533, 577, 1505, 1780, 1824, 1840 |
| MlyI | GAGTC | 3 cut(s) | 1346, 1734, 1874 |
| MmeI | TCCRAC | 2 cut(s) | 55, 1139 |
| Mph1103I | ATGCAT | 2 cut(s) | 1042, 1321 |
| MseI | TTAA | 6 cut(s) | 216, 411, 1059, 1479, 1526, 1905 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1242, 1895 |
| MspI | CCGG | 1 cut(s) | 590 |
| MspR9I | CCNGG | 2 cut(s) | 590, 1542 |
| MssI | GTTTAAAC | 1 cut(s) | 217 |
| MunI | CAATTG | 1 cut(s) | 810 |
| MvaI | CCWGG | 1 cut(s) | 1542 |
| MvnI | CGCG | 1 cut(s) | 520 |
| NciI | CCSGG | 1 cut(s) | 590 |
| NcoI | CCATGG | 1 cut(s) | 878 |
| NdeI | CATATG | 1 cut(s) | 1067 |
| NdeII | GATC | 7 cut(s) | 644, 664, 1159, 1165, 1234, 2042, 2089 |
| NlaIV | GGNNCC | 2 cut(s) | 1113, 1474 |
| NmuCI | GTSAC | 2 cut(s) | 361, 1877 |
| NsiI | ATGCAT | 2 cut(s) | 1042, 1321 |
| NspI | RCATGY | 5 cut(s) | 1259, 1443, 1672, 1701, 1807 |
| PaeI | GCATGC | 1 cut(s) | 1672 |
| PagI | TCATGA | 1 cut(s) | 955 |
| PaqCI | CACCTGC | 1 cut(s) | 650 |
| PciI | ACATGT | 2 cut(s) | 1697, 1803 |
| PfeI | GAWTC | 2 cut(s) | 421, 1026 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 647 |
| PkrI | GCNGC | 4 cut(s) | 618, 1203, 1254, 1627 |
| PleI | GAGTC | 3 cut(s) | 1346, 1733, 1874 |
| PmeI | GTTTAAAC | 1 cut(s) | 217 |
| PpsI | GAGTC | 3 cut(s) | 1346, 1733, 1874 |
| Ppu21I | YACGTR | 1 cut(s) | 1500 |
| PscI | ACATGT | 2 cut(s) | 1697, 1803 |
| PshBI | ATTAAT | 1 cut(s) | 1526 |
| Psp6I | CCWGG | 1 cut(s) | 1540 |
| PspFI | CCCAGC | 1 cut(s) | 1651 |
| PspGI | CCWGG | 1 cut(s) | 1540 |
| PspN4I | GGNNCC | 2 cut(s) | 1113, 1474 |
| PspPI | GGNCC | 4 cut(s) | 155, 458, 949, 1544 |
| PstNI | CAGNNNCTG | 1 cut(s) | 616 |
| PsuI | RGATCY | 1 cut(s) | 1165 |
| RsaI | GTAC | 8 cut(s) | 189, 240, 523, 609, 1092, 1474, 2000, 2074 |
| RsaNI | GTAC | 8 cut(s) | 188, 239, 522, 608, 1091, 1473, 1999, 2073 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1242, 1895 |
| SalI | GTCGAC | 1 cut(s) | 1701 |
| SaqAI | TTAA | 6 cut(s) | 216, 411, 1059, 1479, 1526, 1905 |
| SatI | GCNGC | 4 cut(s) | 617, 1202, 1253, 1626 |
| Sau3AI | GATC | 7 cut(s) | 644, 664, 1159, 1165, 1234, 2042, 2089 |
| Sau96I | GGNCC | 4 cut(s) | 155, 458, 949, 1544 |
| SchI | GAGTC | 3 cut(s) | 1346, 1734, 1874 |
| ScrFI | CCNGG | 2 cut(s) | 590, 1542 |
| SduI | GDGCHC | 7 cut(s) | 448, 533, 577, 1505, 1780, 1824, 1840 |
| SfaNI | GCATC | 8 cut(s) | 37, 124, 646, 696, 709, 1027, 1049, 1306 |
| SfcI | CTRYAG | 1 cut(s) | 42 |
| SinI | GGWCC | 2 cut(s) | 458, 949 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1242, 1895 |
| SmlI | CTYRAG | 1 cut(s) | 498 |
| SmoI | CTYRAG | 1 cut(s) | 498 |
| SpeI | ACTAGT | 1 cut(s) | 1984 |
| SphI | GCATGC | 1 cut(s) | 1672 |
| SsiI | CCGC | 4 cut(s) | 518, 1186, 1864, 2068 |
| SspMI | CTAG | 4 cut(s) | 278, 449, 1763, 1985 |
| StyD4I | CCNGG | 2 cut(s) | 588, 1540 |
| StyI | CCWWGG | 6 cut(s) | 175, 878, 1122, 1720, 1831, 1923 |
| TaaI | ACNGT | 7 cut(s) | 418, 629, 766, 1269, 1286, 1392, 1433 |
| TaiI | ACGT | 4 cut(s) | 215, 309, 847, 1502 |
| TaqI | TCGA | 7 cut(s) | 52, 309, 1273, 1608, 1702, 1883, 2088 |
| TatI | WGTACW | 2 cut(s) | 187, 607 |
| TfiI | GAWTC | 2 cut(s) | 421, 1026 |
| Tru1I | TTAA | 6 cut(s) | 216, 411, 1059, 1479, 1526, 1905 |
| Tru9I | TTAA | 6 cut(s) | 216, 411, 1059, 1479, 1526, 1905 |
| TscAI | CASTG | 2 cut(s) | 423, 628 |
| TseFI | GTSAC | 2 cut(s) | 361, 1877 |
| TseI | GCWGC | 4 cut(s) | 616, 1201, 1252, 1625 |
| Tsp45I | GTSAC | 2 cut(s) | 361, 1877 |
| TspDTI | ATGAA | 9 cut(s) | 282, 496, 745, 944, 1043, 1412, 1629, 1698, 2009 |
| TspGWI | ACGGA | 6 cut(s) | 138, 363, 672, 1049, 2016, 2096 |
| TspRI | CASTG | 2 cut(s) | 423, 628 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 647 |
| VneI | GTGCAC | 2 cut(s) | 444, 573 |
| VpaK11BI | GGWCC | 2 cut(s) | 458, 949 |
| VspI | ATTAAT | 1 cut(s) | 1526 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 956 |
| XapI | RAATTY | 2 cut(s) | 227, 803 |
| XbaI | TCTAGA | 1 cut(s) | 1762 |
| XceI | RCATGY | 5 cut(s) | 1259, 1443, 1672, 1701, 1807 |
| XmiI | GTMKAC | 2 cut(s) | 494, 1702 |
| XspI | CTAG | 4 cut(s) | 278, 449, 1763, 1985 |
| ZraI | GACGTC | 1 cut(s) | 307 |
| Zsp2I | ATGCAT | 2 cut(s) | 1042, 1321 |