Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT2G32000 AT2G32000 |
| fragaria_vesca | FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 FvH4_4g12750 |
| malus_domestica | MD04G1023600.v1.1 |
| prunus_persica | Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 Prupe.1G173400_v2.0.a1 |
| pyrus_communis | pycom04g02020 |
| rosa_chinensis | RchiOBHm_Chr4g0412031 |
| rosa_laevigata | RLG00000008327 |
| rosa_multiflora | Rmu_co8041726.1_g000001 Rmu_sc0000711.1_g000051 |
| rosa_roxburghii | Rroxscaffold_1G00042480 Rroxscaffold_5G00346360 Rroxscaffold_5G00356250 Rroxscaffold_5G00356340 |
| rosa_rugosa | Rorug04G0106700 Rorug04G0106800 Rorug04G0106900 |
| rosa_samantha | Rh4AG171100 Rh4BG168800 Rh4BG169700 Rh4CG181900 Rh4DG164200 Rh4DG165300 |
| rosa_wichuraiana | Rw0G007770 Rw0G008800 Rw4G014260 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 364 |
| AccB1I | GGYRCC | 1 cut(s) | 364 |
| AclI | AACGTT | 1 cut(s) | 138 |
| AcsI | RAATTY | 2 cut(s) | 394, 470 |
| AfaI | GTAC | 2 cut(s) | 56, 366 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 286 |
| AgsI | TTSAA | 4 cut(s) | 7, 168, 217, 229 |
| AjnI | CCWGG | 1 cut(s) | 422 |
| AluBI | AGCT | 5 cut(s) | 156, 211, 379, 404, 446 |
| AluI | AGCT | 5 cut(s) | 156, 211, 379, 404, 446 |
| Alw21I | GWGCWC | 1 cut(s) | 381 |
| Ama87I | CYCGRG | 1 cut(s) | 380 |
| ApeKI | GCWGC | 3 cut(s) | 401, 419, 429 |
| ApoI | RAATTY | 2 cut(s) | 394, 470 |
| Asp718I | GGTACC | 1 cut(s) | 364 |
| AspLEI | GCGC | 1 cut(s) | 429 |
| AsuHPI | GGTGA | 1 cut(s) | 203 |
| AsuNHI | GCTAGC | 1 cut(s) | 311 |
| AvaI | CYCGRG | 1 cut(s) | 380 |
| BanI | GGYRCC | 1 cut(s) | 364 |
| BanII | GRGCYC | 1 cut(s) | 381 |
| BbsI | GAAGAC | 1 cut(s) | 54 |
| Bbv12I | GWGCWC | 1 cut(s) | 381 |
| BbvI | GCAGC | 3 cut(s) | 413, 431, 441 |
| BccI | CCATC | 1 cut(s) | 263 |
| BceAI | ACGGC | 1 cut(s) | 417 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 281, 315, 390, 424 |
| BciT130I | CCWGG | 1 cut(s) | 424 |
| BfaI | CTAG | 4 cut(s) | 264, 312, 357, 447 |
| BisI | GCNGC | 3 cut(s) | 402, 420, 430 |
| BlsI | GCNGC | 3 cut(s) | 403, 421, 431 |
| Bme1390I | CCNGG | 1 cut(s) | 424 |
| BmeT110I | CYCGRG | 1 cut(s) | 380 |
| BmiI | GGNNCC | 1 cut(s) | 366 |
| BmrFI | CCNGG | 1 cut(s) | 424 |
| BmsI | GCATC | 2 cut(s) | 31, 117 |
| BmtI | GCTAGC | 1 cut(s) | 315 |
| BpiI | GAAGAC | 1 cut(s) | 54 |
| BpuEI | CTTGAG | 1 cut(s) | 354 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 286 |
| Bse1I | ACTGG | 1 cut(s) | 32 |
| BseBI | CCWGG | 1 cut(s) | 424 |
| BseGI | GGATG | 1 cut(s) | 90 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 286 |
| BseNI | ACTGG | 1 cut(s) | 32 |
| BseXI | GCAGC | 3 cut(s) | 413, 431, 441 |
| BseYI | CCCAGC | 1 cut(s) | 462 |
| BsgI | GTGCAG | 1 cut(s) | 165 |
| BshNI | GGYRCC | 1 cut(s) | 364 |
| BsiHKAI | GWGCWC | 1 cut(s) | 381 |
| BsiHKCI | CYCGRG | 1 cut(s) | 380 |
| BslFI | GGGAC | 3 cut(s) | 219, 262, 479 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 286 |
| BsmFI | GGGAC | 3 cut(s) | 219, 262, 479 |
| BsmI | GAATGC | 1 cut(s) | 224 |
| BsoBI | CYCGRG | 1 cut(s) | 380 |
| Bsp1286I | GDGCHC | 1 cut(s) | 381 |
| BspLI | GGNNCC | 1 cut(s) | 366 |
| BspOI | GCTAGC | 1 cut(s) | 315 |
| BspQI | GCTCTTC | 1 cut(s) | 201 |
| BspT107I | GGYRCC | 1 cut(s) | 364 |
| BsrI | ACTGG | 1 cut(s) | 32 |
| Bst2UI | CCWGG | 1 cut(s) | 424 |
| Bst4CI | ACNGT | 3 cut(s) | 127, 271, 478 |
| Bst6I | CTCTTC | 2 cut(s) | 98, 201 |
| BstC8I | GCNNGC | 1 cut(s) | 313 |
| BstDEI | CTNAG | 1 cut(s) | 305 |
| BstF5I | GGATG | 1 cut(s) | 90 |
| BstHHI | GCGC | 1 cut(s) | 429 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 28, 308 |
| BstNI | CCWGG | 1 cut(s) | 424 |
| BstSCI | CCNGG | 1 cut(s) | 422 |
| BstV1I | GCAGC | 3 cut(s) | 413, 431, 441 |
| BstV2I | GAAGAC | 1 cut(s) | 54 |
| BtgZI | GCGATG | 1 cut(s) | 94 |
| BtsCI | GGATG | 1 cut(s) | 90 |
| BtsI | GCAGTG | 1 cut(s) | 392 |
| BtsIMutI | CAGTG | 1 cut(s) | 392 |
| Cac8I | GCNNGC | 1 cut(s) | 313 |
| CfoI | GCGC | 1 cut(s) | 429 |
| Csp6I | GTAC | 2 cut(s) | 55, 365 |
| CviAII | CATG | 1 cut(s) | 114 |
| CviQI | GTAC | 2 cut(s) | 55, 365 |
| DdeI | CTNAG | 1 cut(s) | 305 |
| Eam1104I | CTCTTC | 2 cut(s) | 98, 201 |
| EarI | CTCTTC | 2 cut(s) | 98, 201 |
| Ecl136II | GAGCTC | 1 cut(s) | 379 |
| Eco24I | GRGCYC | 1 cut(s) | 381 |
| Eco53kI | GAGCTC | 1 cut(s) | 379 |
| Eco88I | CYCGRG | 1 cut(s) | 380 |
| EcoICRI | GAGCTC | 1 cut(s) | 379 |
| EcoRI | GAATTC | 1 cut(s) | 394 |
| EcoRII | CCWGG | 1 cut(s) | 422 |
| EcoT38I | GRGCYC | 1 cut(s) | 381 |
| FaeI | CATG | 1 cut(s) | 117 |
| FaiI | YATR | 6 cut(s) | 44, 115, 159, 201, 275, 481 |
| FaqI | GGGAC | 3 cut(s) | 219, 262, 479 |
| FatI | CATG | 1 cut(s) | 113 |
| Fnu4HI | GCNGC | 3 cut(s) | 402, 420, 430 |
| FokI | GGATG | 1 cut(s) | 97 |
| FriOI | GRGCYC | 1 cut(s) | 381 |
| Fsp4HI | GCNGC | 3 cut(s) | 402, 420, 430 |
| FspBI | CTAG | 4 cut(s) | 264, 312, 357, 447 |
| GlaI | GCGC | 1 cut(s) | 428 |
| GluI | GCNGC | 3 cut(s) | 402, 420, 430 |
| GsaI | CCCAGC | 1 cut(s) | 466 |
| HhaI | GCGC | 1 cut(s) | 429 |
| Hin1II | CATG | 1 cut(s) | 117 |
| Hin6I | GCGC | 1 cut(s) | 427 |
| HinP1I | GCGC | 1 cut(s) | 427 |
| HphI | GGTGA | 1 cut(s) | 203 |
| Hpy188I | TCNGA | 1 cut(s) | 247 |
| HpyCH4III | ACNGT | 3 cut(s) | 127, 271, 478 |
| HpyCH4IV | ACGT | 1 cut(s) | 138 |
| HpyCH4V | TGCA | 1 cut(s) | 146 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 28, 308 |
| HpyF3I | CTNAG | 1 cut(s) | 305 |
| HpySE526I | ACGT | 1 cut(s) | 138 |
| Hsp92II | CATG | 1 cut(s) | 117 |
| HspAI | GCGC | 1 cut(s) | 427 |
| KpnI | GGTACC | 1 cut(s) | 368 |
| LguI | GCTCTTC | 1 cut(s) | 201 |
| LpnPI | CCDG | 5 cut(s) | 45, 409, 422, 436, 448 |
| Lsp1109I | GCAGC | 3 cut(s) | 413, 431, 441 |
| LweI | GCATC | 2 cut(s) | 31, 117 |
| MaeI | CTAG | 4 cut(s) | 264, 312, 357, 447 |
| MaeII | ACGT | 1 cut(s) | 138 |
| MboII | GAAGA | 5 cut(s) | 59, 85, 156, 218, 399 |
| MhlI | GDGCHC | 1 cut(s) | 381 |
| MluCI | AATT | 2 cut(s) | 394, 470 |
| MnlI | CCTC | 5 cut(s) | 61, 182, 247, 355, 378 |
| MseI | TTAA | 2 cut(s) | 63, 182 |
| MslI | CAYNNNNRTG | 2 cut(s) | 229, 456 |
| MspR9I | CCNGG | 1 cut(s) | 424 |
| Mva1269I | GAATGC | 1 cut(s) | 224 |
| MvaI | CCWGG | 1 cut(s) | 424 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 28, 308 |
| NheI | GCTAGC | 1 cut(s) | 311 |
| NlaIII | CATG | 1 cut(s) | 117 |
| NlaIV | GGNNCC | 1 cut(s) | 366 |
| PaeR7I | CTCGAG | 1 cut(s) | 380 |
| PciSI | GCTCTTC | 1 cut(s) | 201 |
| PctI | GAATGC | 1 cut(s) | 224 |
| PkrI | GCNGC | 3 cut(s) | 403, 421, 431 |
| Psp124BI | GAGCTC | 1 cut(s) | 381 |
| Psp1406I | AACGTT | 1 cut(s) | 138 |
| Psp6I | CCWGG | 1 cut(s) | 422 |
| PspFI | CCCAGC | 1 cut(s) | 462 |
| PspGI | CCWGG | 1 cut(s) | 422 |
| PspN4I | GGNNCC | 1 cut(s) | 366 |
| PspXI | VCTCGAGB | 1 cut(s) | 380 |
| RsaI | GTAC | 2 cut(s) | 56, 366 |
| RsaNI | GTAC | 2 cut(s) | 55, 365 |
| RseI | CAYNNNNRTG | 2 cut(s) | 229, 456 |
| SacI | GAGCTC | 1 cut(s) | 381 |
| SapI | GCTCTTC | 1 cut(s) | 201 |
| SaqAI | TTAA | 2 cut(s) | 63, 182 |
| SatI | GCNGC | 3 cut(s) | 402, 420, 430 |
| ScrFI | CCNGG | 1 cut(s) | 424 |
| SduI | GDGCHC | 1 cut(s) | 381 |
| SfaNI | GCATC | 2 cut(s) | 31, 117 |
| Sfr274I | CTCGAG | 1 cut(s) | 380 |
| SlaI | CTCGAG | 1 cut(s) | 380 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 229, 456 |
| SmlI | CTYRAG | 2 cut(s) | 369, 380 |
| SmoI | CTYRAG | 2 cut(s) | 369, 380 |
| Sse9I | AATT | 2 cut(s) | 394, 470 |
| SspMI | CTAG | 4 cut(s) | 264, 312, 357, 447 |
| SstI | GAGCTC | 1 cut(s) | 381 |
| StyD4I | CCNGG | 1 cut(s) | 422 |
| TaaI | ACNGT | 3 cut(s) | 127, 271, 478 |
| TaiI | ACGT | 1 cut(s) | 141 |
| TaqI | TCGA | 3 cut(s) | 291, 381, 410 |
| TasI | AATT | 2 cut(s) | 394, 470 |
| TatI | WGTACW | 1 cut(s) | 54 |
| Tru1I | TTAA | 2 cut(s) | 63, 182 |
| Tru9I | TTAA | 2 cut(s) | 63, 182 |
| TscAI | CASTG | 1 cut(s) | 392 |
| TseI | GCWGC | 3 cut(s) | 401, 419, 429 |
| TspDTI | ATGAA | 1 cut(s) | 85 |
| TspRI | CASTG | 1 cut(s) | 392 |
| XapI | RAATTY | 2 cut(s) | 394, 470 |
| XhoI | CTCGAG | 1 cut(s) | 380 |
| XspI | CTAG | 4 cut(s) | 264, 312, 357, 447 |