MD04G1045000.v1.1

Flowering time control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002760
Physical Location & Seq
Reverse (-)
5238225 .. 5251657
13433 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1045000.v1.1.491

Sequence Viewer

Length: 2982 bp
ATGGCGCCTGCGGCCAAACCGAACAAGCAAGGCACAGACGATTCCGAGACGCCGTCGAACAATCTCTGGGTTGGAAATTTAGCGAGCGACGTCACCGACGCCGATCTCATGGACCTGTTTGCCCAATTCGGTGCTTTGGATAGCGTCACCTCCTACTCGTCACGGAGCTACGCCTTCGTGTTCTTCAAGCGCGTGGAGGACGCTGCGGCGGCCAAGGAGGCATTGCAAGGTGCCCTTCTGCGCGGAAACCCTGTTAAGATTGAGTTTGCCCGACCGGCAAAACCTTGCAAGAATCTATGGGTAGGTGGAATTAGCCCATCTGTCTCAAAGGAAGAATTAGAAGAAGAGTTTTGTAAGTTTGGAAAAATGGAGGACTTCAAGTTCCTTAGAGACTGTAATACTGCATTCGTTGAATATTTTAAATTGGAGGATGCTTCCCAAGCAATGAGAAACATGAACGGAAAGAGATTGGGTGGTGACCAGATACGCGTTGATTATCTTCGATCACAATACTCGAGAAGGGAACAGCCTGACTACAGAGATGGACAGTTTCTGGCCAGAAACATGGGGCCTGCTGATTCACATAAAAGACAACAGTATTCTCAATCTTCCGGGGGACGAAAAGGTGACAGTCAACCCAGTAATGTTCTGTGGGTGGGCTATCCTCCTTCTGTGCAGATTGATGAACAAATGCTCCACAATGCCATGATTTTGTTTGGTGAGATTGAGAGAATTAAAAGTTTTCCTTCAAGACACTACTCCTTTGTCGAATTCAGGAGTGTGGATGAAGCTCGGCGGGCCAAGGAGGGCTTGCAAGGCAGGCTTTTCAATGATCCTCGGATTACAATTATGTTCTCAAGCAGTGGGCTGGCACCTGGGAAAGAATACCCTGGCCCTTATCCTGGAGGCCAAGGACCTAGGTCAGATATGTTATTCAATGAACATCCTTTTCAGTCTTTACCAATGGACATGTTTGGTCATAACCGTCCAATGATGTCTAATAATTATCCTGGAGCTCTACCACAAAATGGCATTCTTGGACCAAATGCTCCAATGAGGCCTTTAGGTCCTCAAGGTAGGTTTGACCCTCTGCTTTCAGGTCCAGAACTGAATGATTTGGCATCGCTTCATAATTATCAAGACGGAAATTCCAAGAACCTGATGGGTCCCAACTGGAGGCAGCTGTCTCCTCCCACACCTAGGGCCGTGTCTTCTCCTGTGCCTGGTATTAGGCCACCCACGAGACCTGCATCTAGTGCATGGGATGTGTTAGATGCTAACCAATTTCAAAAGGATGCTAAACGATCAAGAATAGATAGTCCTTTGTCCATGGACGACCCTCCATATCAGTTTAGAAACGTTGATGACCATGGATTAGGGTTCGACTCGTCTTACGGACTTGGTCCGGTAATTGATGGAGGTGCTTCTGGACCATCTATGAATGTCCAAGGGAAGAATCGCCTTAGTCCAGCTGGTGTAAGGGTCTCAGTTGGAGGGCCTCCTGAAAATGATTTTATATGGCGTGGAACTATTGCCAAGGGTGGAACACCTGTTTGCCATGCTCGCTGTGTCCCTATAGGAAAAGGAATACGAAATGAGCTTCCTGAAGTTGTTAATTGTTCGGCTAGAACTGGACTGGATATGCTCACAAAACACTATGCTGAGGCCATTGGATTTGATATCGTTTTCTTCTTGCCAGATAGTGAAGATGATTTTGCTTCCTACACTGAATTCCTACGTTATCTAGGTGCAAAAAACCGTGCTGGTGTTGCAAAGTTTGATGATGGGATGACCTTATTTTTGGTGCCTCCTTCAGATTTCTTGAAGAATGTTTTAAAAGTTGCAGGCCCTGAACGTCTGTATGGTGTGGTTCTCAAGTTTCCACCAGTTCCTAGTACTGCTTCTATGCACGAACAGATGCAGCCCATGCCACCTTCACAGTTTATAGATAGACAGCAGATTCCTTCTTCACAAGTTGAGTATAGTGTAATCCCACCGAAGGAGGATCATATTTTGCATATGGATTATAACAGGGTTTTGTATGAGGACTCAAAGCGTTCTGCAAAACCACTGTTTCCGCCTACTAGCGAGTCCTCTAGAGGGCAACTGCAAGACTATGCTTCTAGTAATAGTACTGCAGTATCACAAGCTGGAGTTGCTTTAACTCCTGAACTTATTGCCACTCTGGCAACTTTACTGCCTGGAAATGCACAGCCGTCTGGTCCAGAAGGTGCTAGGGTACCAGTATCCTCAGCAGCTAGGCATTCATTTCCTGCTTTTGCACCTAGTGAAGTATCTTCTCCTGGATGGAAACAAGATCAACAAATTTCTGATCATACCGGTCATGCATTACAACAATTAGGTAATCAGTTTAATCCACACGAACAGAATCATTCGCAGTACCAACCTTATCCATCTGTTCCAAACTCATCCAACCACTCTACCCCACTAGCCCCTGGTATCAACCAATTCCCAGACTCTTCTACCAGTCAGCCCTCGCAGTCTGCAAATTCATCTAGGCCTTTGAATAATTTTACTATCCCTTCTCAAGGCGGACAAACTTTTGGACCCTCTCATCTCAACCAGCACTATCTGGCTGAAGCTCCTCTTGGTACTCAGAAAGGCTTTTCAGCACATGGAACAGATACTTCTGTGTTGTACAATCCACCTGTTTCTCAGCAACACAATAATTCTATGGCCTTTTCTGGTCAGACTTATGGTGCTAATTCGCAGTCTCAGACCTTTCTGCCAGTAGCGGCTGAAAAGGTGAACCCAGAATACCCAAATCAAATGCAGCAGCTTCAGCCTTCACTTGGGGCTGGGGCTGGTCAGAGCGCCCCAGATGGTGAGGCAGACAAAAATCATCGGTATCAGTCGACGCTCCAATTTGCGGCTAACCTCCTCCTTCAATTACAGCAGCAACAACAGCAGCAGCAGCAAATGGGTAGTCAAGCGGGGCAAGGGTCTGGAAGTCAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0000785 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006325 GO:0006342 GO:0006355 GO:0006378 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009553 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0019219 GO:0019222 GO:0022414 GO:0031047 GO:0031048 GO:0031123 GO:0031124 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0034641 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
RRM_1 SPOC

Protein Analysis

994

Amino Acids

108.57

Weight (kDa)

6.18

Isoelectric Point (pI)

59.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 22 - 85 4.1e-13 RNA recognition motif
RRM_1 PF00076 99 - 163 8.8e-13 RNA recognition motif
RRM_1 PF00076 227 - 281 5.4e-07 RNA recognition motif
SPOC PF07744 501 - 626 7e-26 SPOC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2028
AatII GACGTC 1 cut(s) 93
Acc36I ACCTGC 1 cut(s) 1255
Acc65I GGTACC 1 cut(s) 2239
AccB1I GGYRCC 5 cut(s) 4, 230, 871, 1804, 2239
AccB7I CCANNNNNTGG 1 cut(s) 1028
AccI GTMKAC 1 cut(s) 2876
AccII CGCG 3 cut(s) 192, 243, 489
AclI AACGTT 1 cut(s) 1359
AclWI GGATC 2 cut(s) 827, 2013
AcoI YGGCCR 3 cut(s) 12, 210, 555
AcsI RAATTY 6 cut(s) 76, 770, 1147, 1730, 2325, 2509
AcuI CTGAAG 4 cut(s) 1626, 1797, 2619, 2786
AcyI GRCGYC 4 cut(s) 5, 50, 90, 99
AdeI CACNNNGTG 1 cut(s) 2288
AfaI GTAC 6 cut(s) 1897, 2134, 2241, 2402, 2614, 2660
AflIII ACRYGT 2 cut(s) 487, 969
AgeI ACCGGT 1 cut(s) 2339
AjnI CCWGG 8 cut(s) 874, 889, 901, 1009, 1222, 2200, 2302, 2455
AjuI GAANNNNNNNTTGG 2 cut(s) 2592, 2624
AloI GAACNNNNNNTCC 4 cut(s) 365, 397, 678, 710
Alw21I GWGCWC 1 cut(s) 1018
Alw26I GTCTC 7 cut(s) 41, 328, 384, 1191, 1237, 1489, 2739
AlwI GGATC 2 cut(s) 827, 2013
AlwNI CAGNNNCTG 2 cut(s) 553, 1850
Ama87I CYCGRG 1 cut(s) 514
ApoI RAATTY 6 cut(s) 76, 770, 1147, 1730, 2325, 2509
ArsI GACNNNNNNTTYG 4 cut(s) 1783, 1815, 2932, 2964
AsiGI ACCGGT 1 cut(s) 2339
Asp718I GGTACC 1 cut(s) 2239
AspA2I CCTAGG 2 cut(s) 917, 1199
AspLEI GCGC 4 cut(s) 7, 192, 243, 2837
AsuC2I CCSGG 1 cut(s) 613
AsuHPI GGTGA 7 cut(s) 85, 139, 488, 638, 731, 2779, 2858
AvaI CYCGRG 1 cut(s) 514
AvrII CCTAGG 2 cut(s) 917, 1199
BaeGI GKGCMC 1 cut(s) 235
BaeI ACNNNNGTAYC 4 cut(s) 2124, 2157, 2637, 2670
BalI TGGCCA 1 cut(s) 557
BanI GGYRCC 5 cut(s) 4, 230, 871, 1804, 2239
BanII GRGCYC 1 cut(s) 1018
BauI CACGAG 1 cut(s) 1240
BbsI GAAGAC 1 cut(s) 1203
Bbv12I GWGCWC 1 cut(s) 1018
BbvCI CCTCAGC 2 cut(s) 1662, 2251
BccI CCATC 9 cut(s) 325, 536, 1156, 1409, 1441, 1778, 2301, 2422, 2837
BceAI ACGGC 3 cut(s) 37, 1190, 2200
BciT130I CCWGG 8 cut(s) 876, 891, 903, 1011, 1224, 2202, 2304, 2457
BciVI GTATCC 1 cut(s) 2257
BclI TGATCA 1 cut(s) 2332
BcnI CCSGG 1 cut(s) 613
BcoDI GTCTC 7 cut(s) 41, 328, 384, 1191, 1237, 1489, 2739
BfmI CTRYAG 3 cut(s) 535, 1575, 2136
BfoI RGCGCY 2 cut(s) 8, 2838
BfuAI ACCTGC 1 cut(s) 1255
BfuI GTATCC 1 cut(s) 2257
BglI GCCNNNNNGGC 3 cut(s) 218, 275, 2186
BlnI CCTAGG 2 cut(s) 917, 1199
BmcAI AGTACT 2 cut(s) 1897, 2134
Bme1390I CCNGG 9 cut(s) 613, 876, 891, 903, 1011, 1224, 2202, 2304, 2457
BmeT110I CYCGRG 1 cut(s) 514
BmiI GGNNCC 9 cut(s) 6, 232, 570, 873, 1167, 1168, 1806, 2241, 2569
BmrFI CCNGG 9 cut(s) 613, 876, 891, 903, 1011, 1224, 2202, 2304, 2457
BmrI ACTGGG 1 cut(s) 633
BmsI GCATC 6 cut(s) 421, 1130, 1259, 1264, 1285, 1908
BmuI ACTGGG 1 cut(s) 633
BoxI GACNNNNGTC 1 cut(s) 919
BpiI GAAGAC 1 cut(s) 1203
BpmI CTGGAG 4 cut(s) 924, 1032, 1195, 2172
Bpu10I CCTNAGC 2 cut(s) 1662, 2251
BpuEI CTTGAG 4 cut(s) 841, 1056, 1859, 2532
BpuMI CCSGG 1 cut(s) 613
BsaHI GRCGYC 4 cut(s) 5, 50, 90, 99
BsaI GGTCTC 2 cut(s) 1237, 1489
BsaWI WCCGGW 2 cut(s) 1405, 2339
BsaXI ACNNNNNCTCC 2 cut(s) 678, 708
Bse118I RCCGGY 2 cut(s) 274, 2339
Bse1I ACTGG 8 cut(s) 639, 1178, 1636, 1641, 1886, 2243, 2487, 2750
Bse3DI GCAATG 2 cut(s) 221, 450
BseBI CCWGG 8 cut(s) 876, 891, 903, 1011, 1224, 2202, 2304, 2457
BseGI GGATG 8 cut(s) 436, 790, 943, 1270, 1300, 1794, 2312, 2429
BseMI GCAATG 2 cut(s) 221, 450
BseMII CTCAG 6 cut(s) 1500, 1653, 2265, 2630, 2690, 2750
BseNI ACTGG 8 cut(s) 639, 1178, 1636, 1641, 1886, 2243, 2487, 2750
BseRI GAGGAG 3 cut(s) 1179, 2595, 2891
BseSI GKGCMC 1 cut(s) 235
BseYI CCCAGC 1 cut(s) 2819
BsgI GTGCAG 1 cut(s) 695
Bsh1236I CGCG 3 cut(s) 192, 243, 489
Bsh1285I CGRYCG 1 cut(s) 275
BshNI GGYRCC 5 cut(s) 4, 230, 871, 1804, 2239
BshTI ACCGGT 1 cut(s) 2339
BsiEI CGRYCG 1 cut(s) 275
BsiHKAI GWGCWC 1 cut(s) 1018
BsiHKCI CYCGRG 1 cut(s) 514
BsiSI CCGG 4 cut(s) 275, 612, 1406, 2340
BslFI GGGAC 3 cut(s) 630, 1152, 1556
BsmAI GTCTC 7 cut(s) 41, 328, 384, 1191, 1237, 1489, 2739
BsmBI CGTCTC 1 cut(s) 41
BsmFI GGGAC 3 cut(s) 630, 1152, 1556
BsmI GAATGC 3 cut(s) 404, 1032, 2263
Bso31I GGTCTC 2 cut(s) 1237, 1489
BsoBI CYCGRG 1 cut(s) 514
Bsp1286I GDGCHC 2 cut(s) 235, 1018
Bsp1407I TGTACA 1 cut(s) 2658
Bsp143I GATC 7 cut(s) 103, 503, 832, 1304, 2005, 2317, 2332
Bsp19I CCATGG 2 cut(s) 1329, 1369
BspCNI CTCAG 6 cut(s) 1499, 1654, 2264, 2629, 2689, 2749
BspFNI CGCG 3 cut(s) 192, 243, 489
BspLI GGNNCC 9 cut(s) 6, 232, 570, 873, 1167, 1168, 1806, 2241, 2569
BspMAI CTGCAG 1 cut(s) 2140
BspMI ACCTGC 1 cut(s) 1255
BspPI GGATC 2 cut(s) 827, 2013
BspT107I GGYRCC 5 cut(s) 4, 230, 871, 1804, 2239
BspTNI GGTCTC 2 cut(s) 1237, 1489
BsrDI GCAATG 2 cut(s) 221, 450
BsrFI RCCGGY 2 cut(s) 274, 2339
BsrGI TGTACA 1 cut(s) 2658
BsrI ACTGG 8 cut(s) 639, 1178, 1636, 1641, 1886, 2243, 2487, 2750
BssAI RCCGGY 2 cut(s) 274, 2339
BssMI GATC 7 cut(s) 103, 503, 832, 1304, 2005, 2317, 2332
BssNI GRCGYC 4 cut(s) 5, 50, 90, 99
BssSI CACGAG 1 cut(s) 1240
BssT1I CCWWGG 9 cut(s) 213, 801, 910, 917, 1199, 1329, 1369, 1447, 1536
Bst2BI CACGAG 1 cut(s) 1240
Bst2UI CCWGG 8 cut(s) 876, 891, 903, 1011, 1224, 2202, 2304, 2457
Bst4CI ACNGT 8 cut(s) 395, 549, 597, 632, 986, 1760, 1941, 2073
Bst6I CTCTTC 2 cut(s) 339, 2485
BstACI GRCGYC 4 cut(s) 5, 50, 90, 99
BstAPI GCANNNNNTGC 2 cut(s) 1256, 1927
BstAUI TGTACA 1 cut(s) 2658
BstC8I GCNNGC 9 cut(s) 9, 85, 573, 798, 812, 821, 870, 1564, 1846
BstDEI CTNAG 8 cut(s) 386, 1463, 1486, 1662, 2251, 2616, 2676, 2736
BstDSI CCRYGG 2 cut(s) 1329, 1369
BstEII GGTNACC 1 cut(s) 476
BstENI CCTNNNNNAGG 1 cut(s) 2547
BstF5I GGATG 8 cut(s) 436, 790, 943, 1270, 1300, 1794, 2312, 2429
BstFNI CGCG 3 cut(s) 192, 243, 489
BstH2I RGCGCY 2 cut(s) 8, 2838
BstHHI GCGC 4 cut(s) 7, 192, 243, 2837
BstKTI GATC 7 cut(s) 106, 506, 835, 1307, 2008, 2320, 2335
BstMAI GTCTC 7 cut(s) 41, 328, 384, 1191, 1237, 1489, 2739
BstMBI GATC 7 cut(s) 103, 503, 832, 1304, 2005, 2317, 2332
BstMCI CGRYCG 1 cut(s) 275
BstNI CCWGG 8 cut(s) 876, 891, 903, 1011, 1224, 2202, 2304, 2457
BstNSI RCATGY 1 cut(s) 973
BstPAI GACNNNNGTC 1 cut(s) 919
BstPI GGTNACC 1 cut(s) 476
BstSCI CCNGG 9 cut(s) 611, 874, 889, 901, 1009, 1222, 2200, 2302, 2455
BstSFI CTRYAG 3 cut(s) 535, 1575, 2136
BstSLI GKGCMC 1 cut(s) 235
BstUI CGCG 3 cut(s) 192, 243, 489
BstV2I GAAGAC 1 cut(s) 1203
BstXI CCANNNNNNTGG 1 cut(s) 565
BsuI GTATCC 1 cut(s) 2257
BtgI CCRYGG 2 cut(s) 1329, 1369
BtgZI GCGATG 1 cut(s) 1107
BtsCI GGATG 8 cut(s) 436, 790, 943, 1270, 1300, 1794, 2312, 2429
BtsI GCAGTG 1 cut(s) 868
BtsIMutI CAGTG 3 cut(s) 868, 1725, 2069
BveI ACCTGC 1 cut(s) 1255
Cac8I GCNNGC 9 cut(s) 9, 85, 573, 798, 812, 821, 870, 1564, 1846
CaiI CAGNNNCTG 2 cut(s) 553, 1850
CfoI GCGC 4 cut(s) 7, 192, 243, 2837
Cfr10I RCCGGY 2 cut(s) 274, 2339
CseI GACGC 5 cut(s) 58, 107, 133, 209, 2887
Csp6I GTAC 6 cut(s) 1896, 2133, 2240, 2401, 2613, 2659
CspAI ACCGGT 1 cut(s) 2339
CviQI GTAC 6 cut(s) 1896, 2133, 2240, 2401, 2613, 2659
DdeI CTNAG 8 cut(s) 386, 1463, 1486, 1662, 2251, 2616, 2676, 2736
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 105, 505, 834, 1306, 2007, 2319, 2334
DpnII GATC 7 cut(s) 103, 503, 832, 1304, 2005, 2317, 2332
DraI TTTAAA 2 cut(s) 421, 1836
DraIII CACNNNGTG 1 cut(s) 2288
EaeI YGGCCR 3 cut(s) 12, 210, 555
Eam1104I CTCTTC 2 cut(s) 339, 2485
EarI CTCTTC 2 cut(s) 339, 2485
EciI GGCGGA 2 cut(s) 2067, 2568
Ecl136II GAGCTC 1 cut(s) 1016
Eco130I CCWWGG 9 cut(s) 213, 801, 910, 917, 1199, 1329, 1369, 1447, 1536
Eco147I AGGCCT 2 cut(s) 1060, 2521
Eco24I GRGCYC 1 cut(s) 1018
Eco31I GGTCTC 2 cut(s) 1237, 1489
Eco32I GATATC 1 cut(s) 1681
Eco53kI GAGCTC 1 cut(s) 1016
Eco57I CTGAAG 4 cut(s) 1626, 1797, 2619, 2786
Eco88I CYCGRG 1 cut(s) 514
Eco91I GGTNACC 1 cut(s) 476
EcoICRI GAGCTC 1 cut(s) 1016
EcoNI CCTNNNNNAGG 1 cut(s) 2547
EcoO109I RGGNCCY 6 cut(s) 569, 914, 1067, 1166, 1496, 1847
EcoO65I GGTNACC 1 cut(s) 476
EcoRI GAATTC 2 cut(s) 770, 1730
EcoRII CCWGG 8 cut(s) 874, 889, 901, 1009, 1222, 2200, 2302, 2455
EcoRV GATATC 1 cut(s) 1681
EcoT14I CCWWGG 9 cut(s) 213, 801, 910, 917, 1199, 1329, 1369, 1447, 1536
EcoT22I ATGCAT 1 cut(s) 2350
EcoT38I GRGCYC 1 cut(s) 1018
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 9 cut(s) 213, 801, 910, 917, 1199, 1329, 1369, 1447, 1536
Esp3I CGTCTC 1 cut(s) 41
FaqI GGGAC 3 cut(s) 630, 1152, 1556
FauI CCCGC 2 cut(s) 789, 2947
FauNDI CATATG 1 cut(s) 2019
FbaI TGATCA 1 cut(s) 2332
FblI GTMKAC 1 cut(s) 2876
FokI GGATG 8 cut(s) 443, 797, 930, 1277, 1307, 1801, 2319, 2416
FriOI GRGCYC 1 cut(s) 1018
GlaI GCGC 4 cut(s) 6, 191, 242, 2836
GsaI CCCAGC 1 cut(s) 2823
GsuI CTGGAG 4 cut(s) 924, 1032, 1195, 2172
HaeII RGCGCY 2 cut(s) 8, 2838
HapII CCGG 4 cut(s) 275, 612, 1406, 2340
HgaI GACGC 5 cut(s) 58, 107, 133, 209, 2887
HhaI GCGC 4 cut(s) 7, 192, 243, 2837
Hin1I GRCGYC 4 cut(s) 5, 50, 90, 99
Hin6I GCGC 4 cut(s) 5, 190, 241, 2835
HinP1I GCGC 4 cut(s) 5, 190, 241, 2835
HincII GTYRAC 3 cut(s) 635, 2877, 2975
HindII GTYRAC 3 cut(s) 635, 2877, 2975
HpaII CCGG 4 cut(s) 275, 612, 1406, 2340
HphI GGTGA 7 cut(s) 85, 139, 488, 638, 731, 2779, 2858
Hpy166II GTNNAC 4 cut(s) 635, 2770, 2877, 2975
Hpy188I TCNGA 9 cut(s) 46, 840, 925, 1816, 2332, 2619, 2712, 2739, 2832
Hpy8I GTNNAC 4 cut(s) 635, 2770, 2877, 2975
Hpy99I CGWCG 4 cut(s) 58, 92, 101, 2881
HpyCH4III ACNGT 8 cut(s) 395, 549, 597, 632, 986, 1760, 1941, 2073
HpyCH4IV ACGT 4 cut(s) 90, 1359, 1738, 1855
HpyF3I CTNAG 8 cut(s) 386, 1463, 1486, 1662, 2251, 2616, 2676, 2736
HpySE526I ACGT 4 cut(s) 90, 1359, 1738, 1855
Hsp92I GRCGYC 4 cut(s) 5, 50, 90, 99
HspAI GCGC 4 cut(s) 5, 190, 241, 2835
KasI GGCGCC 1 cut(s) 4
KflI GGGWCCC 1 cut(s) 1166
KpnI GGTACC 1 cut(s) 2243
Ksp22I TGATCA 1 cut(s) 2332
Kzo9I GATC 7 cut(s) 103, 503, 832, 1304, 2005, 2317, 2332
LmnI GCTCC 6 cut(s) 165, 699, 1013, 1054, 2608, 2886
LweI GCATC 6 cut(s) 421, 1130, 1259, 1264, 1285, 1908
MaeII ACGT 4 cut(s) 90, 1359, 1738, 1855
MaeIII GTNAC 5 cut(s) 91, 145, 159, 476, 626
MalI GATC 7 cut(s) 105, 505, 834, 1306, 2007, 2319, 2334
MboI GATC 7 cut(s) 103, 503, 832, 1304, 2005, 2317, 2332
MhlI GDGCHC 2 cut(s) 235, 1018
MlsI TGGCCA 1 cut(s) 557
MluI ACGCGT 1 cut(s) 487
MluNI TGGCCA 1 cut(s) 557
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 4 cut(s) 1379, 2042, 2099, 2471
MmeI TCCRAC 3 cut(s) 52, 1471, 2457
Mox20I TGGCCA 1 cut(s) 557
Mph1103I ATGCAT 1 cut(s) 2350
MscI TGGCCA 1 cut(s) 557
MseI TTAA 7 cut(s) 255, 420, 735, 1614, 1835, 2162, 2373
Msp20I TGGCCA 1 cut(s) 557
MspA1I CMGCKG 2 cut(s) 1183, 1472
MspI CCGG 4 cut(s) 275, 612, 1406, 2340
MspR9I CCNGG 9 cut(s) 613, 876, 891, 903, 1011, 1224, 2202, 2304, 2457
Mva1269I GAATGC 3 cut(s) 404, 1032, 2263
MvaI CCWGG 8 cut(s) 876, 891, 903, 1011, 1224, 2202, 2304, 2457
MvnI CGCG 3 cut(s) 192, 243, 489
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 613
NcoI CCATGG 2 cut(s) 1329, 1369
NdeI CATATG 1 cut(s) 2019
NdeII GATC 7 cut(s) 103, 503, 832, 1304, 2005, 2317, 2332
NlaIV GGNNCC 9 cut(s) 6, 232, 570, 873, 1167, 1168, 1806, 2241, 2569
NmeAIII GCCGAG 1 cut(s) 772
NmuCI GTSAC 5 cut(s) 91, 145, 159, 476, 626
NsiI ATGCAT 1 cut(s) 2350
NspI RCATGY 1 cut(s) 973
PaeR7I CTCGAG 1 cut(s) 514
PceI AGGCCT 2 cut(s) 1060, 2521
PciI ACATGT 1 cut(s) 969
PctI GAATGC 3 cut(s) 404, 1032, 2263
PfeI GAWTC 6 cut(s) 41, 292, 578, 1456, 1960, 2389
PflFI GACNNNGTC 2 cut(s) 52, 1401
PflMI CCANNNNNTGG 1 cut(s) 1028
PfoI TCCNGGA 3 cut(s) 901, 1009, 2302
PinAI ACCGGT 1 cut(s) 2339
PleI GAGTC 4 cut(s) 1379, 2042, 2098, 2471
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 4 cut(s) 1379, 2042, 2098, 2471
PpuMI RGGWCCY 3 cut(s) 914, 1067, 1166
PscI ACATGT 1 cut(s) 969
PshAI GACNNNNGTC 1 cut(s) 919
PsiI TTATAA 1 cut(s) 2028
Psp124BI GAGCTC 1 cut(s) 1018
Psp1406I AACGTT 1 cut(s) 1359
Psp5II RGGWCCY 3 cut(s) 914, 1067, 1166
Psp6I CCWGG 8 cut(s) 874, 889, 901, 1009, 1222, 2200, 2302, 2455
PspEI GGTNACC 1 cut(s) 476
PspFI CCCAGC 1 cut(s) 2819
PspGI CCWGG 8 cut(s) 874, 889, 901, 1009, 1222, 2200, 2302, 2455
PspN4I GGNNCC 9 cut(s) 6, 232, 570, 873, 1167, 1168, 1806, 2241, 2569
PspPPI RGGWCCY 3 cut(s) 914, 1067, 1166
PsrI GAACNNNNNNTAC 2 cut(s) 2762, 2794
PstI CTGCAG 1 cut(s) 2140
PstNI CAGNNNCTG 2 cut(s) 553, 1850
PsyI GACNNNGTC 2 cut(s) 52, 1401
PvuII CAGCTG 2 cut(s) 1183, 1472
RsaI GTAC 6 cut(s) 1897, 2134, 2241, 2402, 2614, 2660
RsaNI GTAC 6 cut(s) 1896, 2133, 2240, 2401, 2613, 2659
SacI GAGCTC 1 cut(s) 1018
SalI GTCGAC 1 cut(s) 2875
SaqAI TTAA 7 cut(s) 255, 420, 735, 1614, 1835, 2162, 2373
Sau3AI GATC 7 cut(s) 103, 503, 832, 1304, 2005, 2317, 2332
ScaI AGTACT 2 cut(s) 1897, 2134
SchI GAGTC 4 cut(s) 1379, 2042, 2099, 2471
ScrFI CCNGG 9 cut(s) 613, 876, 891, 903, 1011, 1224, 2202, 2304, 2457
SduI GDGCHC 2 cut(s) 235, 1018
SfaNI GCATC 6 cut(s) 421, 1130, 1259, 1264, 1285, 1908
SfcI CTRYAG 3 cut(s) 535, 1575, 2136
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 514
SlaI CTCGAG 1 cut(s) 514
SmlI CTYRAG 5 cut(s) 514, 856, 1071, 1874, 2547
SmoI CTYRAG 5 cut(s) 514, 856, 1071, 1874, 2547
SseBI AGGCCT 2 cut(s) 1060, 2521
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 416
SstI GAGCTC 1 cut(s) 1018
StuI AGGCCT 2 cut(s) 1060, 2521
StyD4I CCNGG 9 cut(s) 611, 874, 889, 901, 1009, 1222, 2200, 2302, 2455
StyI CCWWGG 9 cut(s) 213, 801, 910, 917, 1199, 1329, 1369, 1447, 1536
TaaI ACNGT 8 cut(s) 395, 549, 597, 632, 986, 1760, 1941, 2073
TaiI ACGT 4 cut(s) 93, 1362, 1741, 1858
TaqI TCGA 6 cut(s) 56, 502, 515, 768, 1383, 2876
TatI WGTACW 3 cut(s) 1895, 2132, 2658
TauI GCSGC 5 cut(s) 14, 209, 212, 2759, 2894
TfiI GAWTC 6 cut(s) 41, 292, 578, 1456, 1960, 2389
Tru1I TTAA 7 cut(s) 255, 420, 735, 1614, 1835, 2162, 2373
Tru9I TTAA 7 cut(s) 255, 420, 735, 1614, 1835, 2162, 2373
TscAI CASTG 3 cut(s) 868, 1732, 2076
TseFI GTSAC 5 cut(s) 91, 145, 159, 476, 626
Tsp45I GTSAC 5 cut(s) 91, 145, 159, 476, 626
TspDTI ATGAA 8 cut(s) 470, 699, 801, 954, 1118, 1454, 2256, 2502
TspGWI ACGGA 4 cut(s) 178, 474, 1158, 1410
TspRI CASTG 3 cut(s) 868, 1732, 2076
Tth111I GACNNNGTC 2 cut(s) 52, 1401
Van91I CCANNNNNTGG 1 cut(s) 1028
XagI CCTNNNNNAGG 1 cut(s) 2547
XapI RAATTY 6 cut(s) 76, 770, 1147, 1730, 2325, 2509
XbaI TCTAGA 1 cut(s) 2096
XceI RCATGY 1 cut(s) 973
XcmI CCANNNNNNNNNTGG 1 cut(s) 1159
XhoI CTCGAG 1 cut(s) 514
XmaJI CCTAGG 2 cut(s) 917, 1199
XmiI GTMKAC 1 cut(s) 2876
ZraI GACGTC 1 cut(s) 91
ZrmI AGTACT 2 cut(s) 1897, 2134
Zsp2I ATGCAT 1 cut(s) 2350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.