pycom13g27540

Flowering time control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002760
Physical Location & Seq
Forward (+)
23752162 .. 23760005
7844 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g27540.1

Sequence Viewer

Length: 2976 bp
ATGGCGCCTCCGACCAAACCCAACAAGCTAGCCACAGACGATTCGGAGACGCCGTCCAACAATCTCTGGGTTGGGAATTTGGCCAGCGACGTCACCGACGCCGATCTCATGGACCTTTTCGCCCAATTCGGTGCTTTGGATAGCGTCACCACCTACTCCTCCCGGAGCTACGCCTTCGTCTTCTTCAAGCGCGTCGAGGACTCCGCCGCTGCCAAGGAGGCATTGCAAGGTGCCCTTCTGCGCGGAAACCCTGTTAAGATTGAGTTTGCCCGACCGGCAAAACCTTGCAAGAATCTATGGGTAGGTGGAATTAGCCCATCTGTCTCAAAGGAAGAATTAGAAGAAGAGTTTTGTAAGTTTGGAAAAATGGAGGACTTCAAGTTCCTTAGAGACCGTAATACTGCATTTGTTGAATATTTTAAATTGGAGGATGCTTCCCAAGCAATGAGAAACATGAACGGGAAGAGATTGGGTGGTGACCAGATACGTGTTGATTATCTTCGATCACAACCCTCGAGAAGAGAACAGTGGCCTGACTACAGAGATGGACAGTTTCCAGCCAGAAACACGGGGCCTGATTCTCACAAAAGACAACAGTATTCTCAATCTTCTGGGGGACGAAAAGGTGACAGCCAACCCAGTAATGTTCTGTGGGTTGGTTATCCTCCTTCTGTTCAGATTGATGAACAAATGCTCCACAATGCCATGATTTTGTTTGGTGAGATTGAGAGAATTAAAAGTTTTCCTTCAAGGCACTACTCCTTTGTTGAATTCAGGAGTGTAGATGAAGCTCGGCGGGCCAAGGATGGCTTGCAAGGCAGGCTTTTCAATGATCCTCGGATTACAATTATGTTCTCAAGCAGTGGGCTGGCACCTGGGAAAGATTACCCTGGCCCTTATCCTGGAGTCAAAGGACCTAGATCAGATATGTTATTCAATGAACATCCGTTTCGGCCTTCACAGATGGACATGTTTGGTCATAACCGTCCAATGATGTCTAATAATTATCCTGGAGCTCTACCACAAAATGGCATTGTTGGACCAAATGCTCCAATGAGGCCTTTAGGTCCTCAAGGTAGGTTTGACCATCTGCTTTCAGGCCCCGAACTTAATGATTTGGCGTCGCTTAATAATTATCAAGATGGAAATTCCAAGAACCTGATGGGTCCCAACTGGAGGCAGCTGTCTCCTCCCACACCTGGGGTCGTGTCTTCTCCTGCGCCTGGTATTAGGTCACACATGAGACCTGCATCTAGTGCATGGGACGTTTTGGATGTTAACCAATTTCAAAGGGATGCTAAACGGTCAAGGATAGATAGTCCTTTGTCCATAGACGACCCTCCATATCCGTTGAGAAAGATTGATGACCATGGATTAGGGTTCGACTCGTCTTATGGACTTGGTCCTGTAATTGATGGAGGTGCTTCTGGACCGTCTATGAATGTCCAAGGGAAGAATCACCTTAGTCCAGCTGGTGTAAGGGTCTCAGTCGGAGGGCTTCCTGATAATGATTTTATATGGCGTGGAACTATTGCTAAGGGTGGAACGCCTGTTTGTCATGCTCGCTGTGTCCCTATAGGAAAAGGAATAGGAAATGAGCTTCCTGGAGTTGTTAATTGTTCGGCTAGAACTGGACTGGATATGCTCACAAAACATTATGCTGAGGCCATTGGATTTGATATAGTATTCTTCTTGCCAGATAGTGAAGATGATTTTGCTTCCTACACTGAATTCCTTCGTTATCTAGGTGCAAAAAACCGTGCTGGTGTTGCGAAGTTTGATGATGGAATGACCTTATTTTTGGTACCTCCTTCAGATTTCTTGAAGGATGTTTTGAAAGTTGCAGGCCCTGAACGTCTGTATGGTGTGGTTCTCAAGTTTCCACAACAAGGTCCTAGTACTGCTTCCATGCACGAACAGATGCAGCCTATGCCACCTTTACAGTTTATAGACAGACAGCAGATTCCTTCTTCACAAGTTGAGTATAGTGTAATCCCACCGAACGATGATCACATTTTACCTATGGATTATAACAGGGTTTTGCATGAGGGCTCGAAGCTTTCTGCAAAACCACTGTTTCCACCTACTAGCAAGTCCTCTAGAGTGCAACCGCAAGACTATGCTTCTAGTAATAGTGCTGCAGTATCACAAGCTGGAGTTTCATTAACTCCTGAACTTATTGCCACTCTGGCCACTTTACTGCCTGGAAATGCACAGCCGTCTGGTCCAGAAGGTGCTAGGGTGCCAGTATCCTCAGCAGCTAGGCATTCATTTCCTGCTTTTGCACCTAGTAAAGTATCCTCTCCTGGATGGAAACAAGATCAACAAATTTCTGATCATACTGGTCATGCATTACAACAGTTTGGTAGTCAGTTTAATCCACACGAACAGAATCTTTTGCAGTACCAGCCTTATCCATCTGTTCCAAACTCATCCAACCATTCTGCCCCAATGGCCCTTGGTATCAACCAAATCCCAGACTCTTCTACCAGTCAGCCCTTGCAGTCTGCAAATCCTTCTAGGCCTTTGAATAATTTTACAATCCCTTCTCAAGGTGGTGGACAGGTTACTGGATCCTCTCATCTCAGCCAGCACTATCTGGCTGAAGCTCCTCTTGGTACTCAGAAATCAGCACATGGAACAGATACATCTGGGTTGTACAATCCACCTGTTTCTCAGCAATATAATAATTCCATGGCCTTTTCTGGTCAGACTTATGGTGCTAATTCGCAGTCTCAGACCTTTCTGCCATTAGCGGCTGAAAAGGTAAACCCAGAATACCCAAATCAAGTGCAGCAACTTCAGTCTGCACTTATTGGGGCTGGTCAGAGCGCCCCAGATGGTGAGGCTGACAAAAATCATCGGTATCAGTCGACGCTCCAATTTGCGGCTAACCTCCTCCTCCAATTACAGCAGCAACAACAGCATAAGCAAACGGGTAGTCAAGCGGGGCGAGGGTCTGGAAGTCAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0000785 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006325 GO:0006342 GO:0006355 GO:0006378 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009553 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0019219 GO:0019222 GO:0022414 GO:0031047 GO:0031048 GO:0031123 GO:0031124 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0034641 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
RRM_1 SPOC

Protein Analysis

992

Amino Acids

108.09

Weight (kDa)

6.68

Isoelectric Point (pI)

55.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 22 - 85 3.4e-12 RNA recognition motif
RRM_1 PF00076 99 - 163 5e-14 RNA recognition motif
RRM_1 PF00076 227 - 281 5.6e-07 RNA recognition motif
SPOC PF07744 501 - 626 6.4e-25 SPOC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2031
AatII GACGTC 1 cut(s) 93
Acc36I ACCTGC 1 cut(s) 1255
Acc65I GGTACC 1 cut(s) 1804
AccB1I GGYRCC 5 cut(s) 4, 230, 871, 1804, 2242
AccB7I CCANNNNNTGG 1 cut(s) 1028
AccI GTMKAC 1 cut(s) 2873
AccII CGCG 2 cut(s) 192, 243
AciI CCGC 8 cut(s) 204, 207, 243, 796, 2111, 2756, 2888, 2948
AclWI GGATC 3 cut(s) 827, 2568, 2581
AcoI YGGCCR 2 cut(s) 81, 2190
AcsI RAATTY 5 cut(s) 76, 770, 1147, 1730, 2328
AcuI CTGAAG 3 cut(s) 1797, 2625, 2786
AcyI GRCGYC 5 cut(s) 5, 50, 90, 99, 1121
AfaI GTAC 5 cut(s) 1806, 1900, 2405, 2620, 2660
AfiI CCNNNNNNNGG 9 cut(s) 902, 1028, 1176, 1199, 1200, 1223, 1889, 2552, 2887
AflIII ACRYGT 2 cut(s) 487, 969
AjnI CCWGG 9 cut(s) 874, 889, 901, 1009, 1198, 1222, 1603, 2203, 2305
AjuI GAANNNNNNNTTGG 2 cut(s) 2598, 2630
AloI GAACNNNNNNTCC 4 cut(s) 365, 397, 678, 710
Alw21I GWGCWC 1 cut(s) 1018
Alw26I GTCTC 7 cut(s) 41, 328, 384, 1191, 1237, 1489, 2739
AlwI GGATC 3 cut(s) 827, 2568, 2581
AlwNI CAGNNNCTG 1 cut(s) 1850
Ama87I CYCGRG 1 cut(s) 514
ApeKI GCWGC 7 cut(s) 209, 1180, 1924, 2138, 2258, 2794, 2914
ApoI RAATTY 5 cut(s) 76, 770, 1147, 1730, 2328
ArsI GACNNNNNNTTYG 4 cut(s) 1783, 1815, 2926, 2958
Asp700I GAANNNNTTC 1 cut(s) 1734
Asp718I GGTACC 1 cut(s) 1804
AspLEI GCGC 5 cut(s) 7, 192, 243, 1222, 2834
AsuC2I CCSGG 1 cut(s) 163
AsuHPI GGTGA 7 cut(s) 85, 139, 488, 638, 731, 1451, 2855
AsuNHI GCTAGC 1 cut(s) 28
AvaI CYCGRG 1 cut(s) 514
AvaII GGWCC 9 cut(s) 112, 914, 1040, 1067, 1166, 1403, 1430, 1892, 2225
BaeGI GKGCMC 1 cut(s) 235
BalI TGGCCA 2 cut(s) 83, 2192
BamHI GGATCC 1 cut(s) 2573
BanI GGYRCC 5 cut(s) 4, 230, 871, 1804, 2242
BanII GRGCYC 2 cut(s) 1018, 2054
BbsI GAAGAC 2 cut(s) 172, 1203
Bbv12I GWGCWC 1 cut(s) 1018
BbvCI CCTCAGC 2 cut(s) 1662, 2254
BbvI GCAGC 7 cut(s) 196, 1192, 1936, 2125, 2270, 2806, 2926
BceAI ACGGC 2 cut(s) 37, 2203
BciT130I CCWGG 9 cut(s) 876, 891, 903, 1011, 1200, 1224, 1605, 2205, 2307
BciVI GTATCC 2 cut(s) 2260, 2308
BclI TGATCA 2 cut(s) 2008, 2335
BcnI CCSGG 1 cut(s) 163
BcoDI GTCTC 7 cut(s) 41, 328, 384, 1191, 1237, 1489, 2739
BfmI CTRYAG 3 cut(s) 538, 1575, 2139
BfoI RGCGCY 2 cut(s) 8, 2835
BfuAI ACCTGC 1 cut(s) 1255
BfuI GTATCC 2 cut(s) 2260, 2308
BglI GCCNNNNNGGC 4 cut(s) 218, 275, 2189, 2453
BmcAI AGTACT 1 cut(s) 1900
Bme18I GGWCC 9 cut(s) 112, 914, 1040, 1067, 1166, 1403, 1430, 1892, 2225
BmeT110I CYCGRG 1 cut(s) 514
BmrI ACTGGG 1 cut(s) 633
BmsI GCATC 4 cut(s) 421, 1259, 1285, 1911
BmtI GCTAGC 1 cut(s) 32
BmuI ACTGGG 1 cut(s) 633
BpiI GAAGAC 2 cut(s) 172, 1203
BpmI CTGGAG 5 cut(s) 924, 1032, 1195, 1626, 2175
Bpu10I CCTNAGC 3 cut(s) 1536, 1662, 2254
BpuEI CTTGAG 4 cut(s) 841, 1056, 1859, 2535
BpuMI CCSGG 1 cut(s) 163
BsaAI YACGTR 1 cut(s) 488
BsaHI GRCGYC 5 cut(s) 5, 50, 90, 99, 1121
BsaI GGTCTC 3 cut(s) 384, 1237, 1489
BsaXI ACNNNNNCTCC 4 cut(s) 38, 68, 678, 708
Bsc4I CCNNNNNNNGG 9 cut(s) 902, 1028, 1176, 1199, 1200, 1223, 1889, 2552, 2887
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 8 cut(s) 639, 1178, 1636, 1641, 2246, 2347, 2490, 2575
Bse3DI GCAATG 2 cut(s) 221, 450
BseBI CCWGG 9 cut(s) 876, 891, 903, 1011, 1200, 1224, 1605, 2205, 2307
BseGI GGATG 8 cut(s) 436, 811, 943, 1279, 1300, 1834, 2315, 2432
BseLI CCNNNNNNNGG 9 cut(s) 902, 1028, 1176, 1199, 1200, 1223, 1889, 2552, 2887
BseMI GCAATG 2 cut(s) 221, 450
BseMII CTCAG 7 cut(s) 1500, 1653, 2268, 2599, 2636, 2690, 2750
BseNI ACTGG 8 cut(s) 639, 1178, 1636, 1641, 2246, 2347, 2490, 2575
BseRI GAGGAG 5 cut(s) 148, 1179, 2601, 2888, 2891
BseSI GKGCMC 1 cut(s) 235
BseXI GCAGC 7 cut(s) 196, 1192, 1936, 2125, 2270, 2806, 2926
BsgI GTGCAG 2 cut(s) 2793, 2813
Bsh1236I CGCG 2 cut(s) 192, 243
Bsh1285I CGRYCG 1 cut(s) 275
BshNI GGYRCC 5 cut(s) 4, 230, 871, 1804, 2242
BsiEI CGRYCG 1 cut(s) 275
BsiHKAI GWGCWC 1 cut(s) 1018
BsiHKCI CYCGRG 1 cut(s) 514
BsiSI CCGG 2 cut(s) 163, 275
BslFI GGGAC 4 cut(s) 630, 1152, 1277, 1556
BslI CCNNNNNNNGG 9 cut(s) 902, 1028, 1176, 1199, 1200, 1223, 1889, 2552, 2887
BsmAI GTCTC 7 cut(s) 41, 328, 384, 1191, 1237, 1489, 2739
BsmBI CGTCTC 1 cut(s) 41
BsmFI GGGAC 4 cut(s) 630, 1152, 1277, 1556
BsmI GAATGC 1 cut(s) 2266
Bso31I GGTCTC 3 cut(s) 384, 1237, 1489
BsoBI CYCGRG 1 cut(s) 514
Bsp1286I GDGCHC 3 cut(s) 235, 1018, 2054
Bsp1407I TGTACA 1 cut(s) 2658
Bsp143I GATC 8 cut(s) 103, 503, 832, 920, 2008, 2320, 2335, 2573
Bsp19I CCATGG 2 cut(s) 1369, 2694
BspACI CCGC 8 cut(s) 204, 207, 243, 796, 2111, 2756, 2888, 2948
BspCNI CTCAG 7 cut(s) 1499, 1654, 2267, 2598, 2635, 2689, 2749
BspFNI CGCG 2 cut(s) 192, 243
BspMAI CTGCAG 1 cut(s) 2143
BspMI ACCTGC 1 cut(s) 1255
BspOI GCTAGC 1 cut(s) 32
BspPI GGATC 3 cut(s) 827, 2568, 2581
BspT107I GGYRCC 5 cut(s) 4, 230, 871, 1804, 2242
BspTNI GGTCTC 3 cut(s) 384, 1237, 1489
BsrDI GCAATG 2 cut(s) 221, 450
BsrFI RCCGGY 1 cut(s) 274
BsrGI TGTACA 1 cut(s) 2658
BsrI ACTGG 8 cut(s) 639, 1178, 1636, 1641, 2246, 2347, 2490, 2575
BssAI RCCGGY 1 cut(s) 274
BssMI GATC 8 cut(s) 103, 503, 832, 920, 2008, 2320, 2335, 2573
BssNI GRCGYC 5 cut(s) 5, 50, 90, 99, 1121
BssT1I CCWWGG 6 cut(s) 213, 801, 1369, 1447, 2458, 2694
Bst2UI CCWGG 9 cut(s) 876, 891, 903, 1011, 1200, 1224, 1605, 2205, 2307
Bst6I CTCTTC 4 cut(s) 339, 458, 514, 2488
BstACI GRCGYC 5 cut(s) 5, 50, 90, 99, 1121
BstAPI GCANNNNNTGC 2 cut(s) 1256, 1930
BstAUI TGTACA 1 cut(s) 2658
BstBAI YACGTR 1 cut(s) 488
BstC8I GCNNGC 9 cut(s) 30, 85, 798, 812, 821, 870, 1564, 1846, 2591
BstDSI CCRYGG 2 cut(s) 1369, 2694
BstEII GGTNACC 1 cut(s) 476
BstENI CCTNNNNNAGG 1 cut(s) 2550
BstF5I GGATG 8 cut(s) 436, 811, 943, 1279, 1300, 1834, 2315, 2432
BstFNI CGCG 2 cut(s) 192, 243
BstH2I RGCGCY 2 cut(s) 8, 2835
BstHHI GCGC 5 cut(s) 7, 192, 243, 1222, 2834
BstKTI GATC 8 cut(s) 106, 506, 835, 923, 2011, 2323, 2338, 2576
BstMAI GTCTC 7 cut(s) 41, 328, 384, 1191, 1237, 1489, 2739
BstMBI GATC 8 cut(s) 103, 503, 832, 920, 2008, 2320, 2335, 2573
BstMCI CGRYCG 1 cut(s) 275
BstNI CCWGG 9 cut(s) 876, 891, 903, 1011, 1200, 1224, 1605, 2205, 2307
BstNSI RCATGY 1 cut(s) 973
BstPI GGTNACC 1 cut(s) 476
BstSFI CTRYAG 3 cut(s) 538, 1575, 2139
BstSLI GKGCMC 1 cut(s) 235
BstUI CGCG 2 cut(s) 192, 243
BstV1I GCAGC 7 cut(s) 196, 1192, 1936, 2125, 2270, 2806, 2926
BstV2I GAAGAC 2 cut(s) 172, 1203
BstX2I RGATCY 1 cut(s) 2573
BstYI RGATCY 1 cut(s) 2573
BsuI GTATCC 2 cut(s) 2260, 2308
BtgI CCRYGG 2 cut(s) 1369, 2694
BtsCI GGATG 8 cut(s) 436, 811, 943, 1279, 1300, 1834, 2315, 2432
BtsI GCAGTG 1 cut(s) 868
BtsIMutI CAGTG 4 cut(s) 533, 868, 1725, 2072
BveI ACCTGC 1 cut(s) 1255
Cac8I GCNNGC 9 cut(s) 30, 85, 798, 812, 821, 870, 1564, 1846, 2591
CaiI CAGNNNCTG 1 cut(s) 1850
CfoI GCGC 5 cut(s) 7, 192, 243, 1222, 2834
Cfr10I RCCGGY 1 cut(s) 274
CseI GACGC 6 cut(s) 58, 107, 133, 181, 1110, 2884
Csp6I GTAC 5 cut(s) 1805, 1899, 2404, 2619, 2659
CviQI GTAC 5 cut(s) 1805, 1899, 2404, 2619, 2659
DinI GGCGCC 1 cut(s) 6
DpnI GATC 8 cut(s) 105, 505, 834, 922, 2010, 2322, 2337, 2575
DpnII GATC 8 cut(s) 103, 503, 832, 920, 2008, 2320, 2335, 2573
DraI TTTAAA 1 cut(s) 421
EaeI YGGCCR 2 cut(s) 81, 2190
Eam1104I CTCTTC 4 cut(s) 339, 458, 514, 2488
EarI CTCTTC 4 cut(s) 339, 458, 514, 2488
EciI GGCGGA 1 cut(s) 193
Ecl136II GAGCTC 1 cut(s) 1016
Eco130I CCWWGG 6 cut(s) 213, 801, 1369, 1447, 2458, 2694
Eco147I AGGCCT 2 cut(s) 1060, 2524
Eco24I GRGCYC 2 cut(s) 1018, 2054
Eco31I GGTCTC 3 cut(s) 384, 1237, 1489
Eco47I GGWCC 9 cut(s) 112, 914, 1040, 1067, 1166, 1403, 1430, 1892, 2225
Eco53kI GAGCTC 1 cut(s) 1016
Eco57I CTGAAG 3 cut(s) 1797, 2625, 2786
Eco88I CYCGRG 1 cut(s) 514
Eco91I GGTNACC 1 cut(s) 476
EcoICRI GAGCTC 1 cut(s) 1016
EcoNI CCTNNNNNAGG 1 cut(s) 2550
EcoO109I RGGNCCY 7 cut(s) 572, 914, 1067, 1100, 1166, 1847, 1892
EcoO65I GGTNACC 1 cut(s) 476
EcoRI GAATTC 2 cut(s) 770, 1730
EcoRII CCWGG 9 cut(s) 874, 889, 901, 1009, 1198, 1222, 1603, 2203, 2305
EcoT14I CCWWGG 6 cut(s) 213, 801, 1369, 1447, 2458, 2694
EcoT22I ATGCAT 1 cut(s) 2353
EcoT38I GRGCYC 2 cut(s) 1018, 2054
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 6 cut(s) 213, 801, 1369, 1447, 2458, 2694
Esp3I CGTCTC 1 cut(s) 41
FaqI GGGAC 4 cut(s) 630, 1152, 1277, 1556
FauI CCCGC 2 cut(s) 789, 2941
FbaI TGATCA 2 cut(s) 2008, 2335
FblI GTMKAC 1 cut(s) 2873
FokI GGATG 8 cut(s) 443, 818, 930, 1286, 1307, 1841, 2322, 2419
FriOI GRGCYC 2 cut(s) 1018, 2054
GlaI GCGC 5 cut(s) 6, 191, 242, 1221, 2833
GsuI CTGGAG 5 cut(s) 924, 1032, 1195, 1626, 2175
HaeII RGCGCY 2 cut(s) 8, 2835
HapII CCGG 2 cut(s) 163, 275
HgaI GACGC 6 cut(s) 58, 107, 133, 181, 1110, 2884
HhaI GCGC 5 cut(s) 7, 192, 243, 1222, 2834
Hin1I GRCGYC 5 cut(s) 5, 50, 90, 99, 1121
Hin6I GCGC 5 cut(s) 5, 190, 241, 1220, 2832
HinP1I GCGC 5 cut(s) 5, 190, 241, 1220, 2832
HincII GTYRAC 3 cut(s) 1279, 2874, 2969
HindII GTYRAC 3 cut(s) 1279, 2874, 2969
HindIII AAGCTT 1 cut(s) 2057
HpaI GTTAAC 1 cut(s) 1279
HpaII CCGG 2 cut(s) 163, 275
HphI GGTGA 7 cut(s) 85, 139, 488, 638, 731, 1451, 2855
Hpy166II GTNNAC 5 cut(s) 1279, 2561, 2770, 2874, 2969
Hpy8I GTNNAC 5 cut(s) 1279, 2561, 2770, 2874, 2969
Hpy99I CGWCG 5 cut(s) 92, 101, 197, 1126, 2878
HpyCH4IV ACGT 4 cut(s) 90, 487, 1266, 1855
HpySE526I ACGT 4 cut(s) 90, 487, 1266, 1855
Hsp92I GRCGYC 5 cut(s) 5, 50, 90, 99, 1121
HspAI GCGC 5 cut(s) 5, 190, 241, 1220, 2832
KasI GGCGCC 1 cut(s) 4
KflI GGGWCCC 1 cut(s) 1166
KpnI GGTACC 1 cut(s) 1808
Ksp22I TGATCA 2 cut(s) 2008, 2335
KspAI GTTAAC 1 cut(s) 1279
Kzo9I GATC 8 cut(s) 103, 503, 832, 920, 2008, 2320, 2335, 2573
LmnI GCTCC 6 cut(s) 165, 699, 1013, 1054, 2614, 2883
Lsp1109I GCAGC 7 cut(s) 196, 1192, 1936, 2125, 2270, 2806, 2926
LweI GCATC 4 cut(s) 421, 1259, 1285, 1911
MaeII ACGT 4 cut(s) 90, 487, 1266, 1855
MaeIII GTNAC 6 cut(s) 91, 145, 476, 626, 1233, 2566
MalI GATC 8 cut(s) 105, 505, 834, 922, 2010, 2322, 2337, 2575
MboI GATC 8 cut(s) 103, 503, 832, 920, 2008, 2320, 2335, 2573
MflI RGATCY 1 cut(s) 2573
MhlI GDGCHC 3 cut(s) 235, 1018, 2054
MlsI TGGCCA 2 cut(s) 83, 2192
MluNI TGGCCA 2 cut(s) 83, 2192
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 4 cut(s) 194, 915, 1379, 2474
MmeI TCCRAC 5 cut(s) 35, 81, 1018, 1471, 2460
Mox20I TGGCCA 2 cut(s) 83, 2192
Mph1103I ATGCAT 1 cut(s) 2353
MroXI GAANNNNTTC 1 cut(s) 1734
MscI TGGCCA 2 cut(s) 83, 2192
MseI TTAA 9 cut(s) 255, 420, 735, 1110, 1128, 1278, 1614, 2165, 2376
Msp20I TGGCCA 2 cut(s) 83, 2192
MspA1I CMGCKG 3 cut(s) 209, 1183, 1472
MspI CCGG 2 cut(s) 163, 275
Mva1269I GAATGC 1 cut(s) 2266
MvaI CCWGG 9 cut(s) 876, 891, 903, 1011, 1200, 1224, 1605, 2205, 2307
MvnI CGCG 2 cut(s) 192, 243
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 163
NcoI CCATGG 2 cut(s) 1369, 2694
NdeII GATC 8 cut(s) 103, 503, 832, 920, 2008, 2320, 2335, 2573
NheI GCTAGC 1 cut(s) 28
NmeAIII GCCGAG 1 cut(s) 772
NmuCI GTSAC 5 cut(s) 91, 145, 476, 626, 1233
NsiI ATGCAT 1 cut(s) 2353
NspI RCATGY 1 cut(s) 973
PaeR7I CTCGAG 1 cut(s) 514
PceI AGGCCT 2 cut(s) 1060, 2524
PciI ACATGT 1 cut(s) 969
PcsI WCGNNNNNNNCGW 1 cut(s) 50
PctI GAATGC 1 cut(s) 2266
PdmI GAANNNNTTC 1 cut(s) 1734
PfeI GAWTC 6 cut(s) 41, 292, 578, 1456, 1963, 2392
PflFI GACNNNGTC 2 cut(s) 52, 1401
PflMI CCANNNNNTGG 1 cut(s) 1028
PfoI TCCNGGA 5 cut(s) 161, 901, 1009, 1603, 2305
PleI GAGTC 4 cut(s) 194, 914, 1379, 2474
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 4 cut(s) 194, 914, 1379, 2474
Ppu21I YACGTR 1 cut(s) 488
PpuMI RGGWCCY 4 cut(s) 914, 1067, 1166, 1892
PscI ACATGT 1 cut(s) 969
PsiI TTATAA 1 cut(s) 2031
Psp124BI GAGCTC 1 cut(s) 1018
Psp5II RGGWCCY 4 cut(s) 914, 1067, 1166, 1892
Psp6I CCWGG 9 cut(s) 874, 889, 901, 1009, 1198, 1222, 1603, 2203, 2305
PspEI GGTNACC 1 cut(s) 476
PspGI CCWGG 9 cut(s) 874, 889, 901, 1009, 1198, 1222, 1603, 2203, 2305
PspPPI RGGWCCY 4 cut(s) 914, 1067, 1166, 1892
PstI CTGCAG 1 cut(s) 2143
PstNI CAGNNNCTG 1 cut(s) 1850
PsuI RGATCY 1 cut(s) 2573
PsyI GACNNNGTC 2 cut(s) 52, 1401
PvuII CAGCTG 2 cut(s) 1183, 1472
RsaI GTAC 5 cut(s) 1806, 1900, 2405, 2620, 2660
RsaNI GTAC 5 cut(s) 1805, 1899, 2404, 2619, 2659
SacI GAGCTC 1 cut(s) 1018
SalI GTCGAC 1 cut(s) 2872
SaqAI TTAA 9 cut(s) 255, 420, 735, 1110, 1128, 1278, 1614, 2165, 2376
Sau3AI GATC 8 cut(s) 103, 503, 832, 920, 2008, 2320, 2335, 2573
ScaI AGTACT 1 cut(s) 1900
SchI GAGTC 4 cut(s) 194, 915, 1379, 2474
SduI GDGCHC 3 cut(s) 235, 1018, 2054
SfaNI GCATC 4 cut(s) 421, 1259, 1285, 1911
SfcI CTRYAG 3 cut(s) 538, 1575, 2139
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 514
SinI GGWCC 9 cut(s) 112, 914, 1040, 1067, 1166, 1403, 1430, 1892, 2225
SlaI CTCGAG 1 cut(s) 514
SmlI CTYRAG 5 cut(s) 514, 856, 1071, 1874, 2550
SmoI CTYRAG 5 cut(s) 514, 856, 1071, 1874, 2550
SseBI AGGCCT 2 cut(s) 1060, 2524
SsiI CCGC 8 cut(s) 204, 207, 243, 796, 2111, 2756, 2888, 2948
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 416
SstI GAGCTC 1 cut(s) 1018
StuI AGGCCT 2 cut(s) 1060, 2524
StyI CCWWGG 6 cut(s) 213, 801, 1369, 1447, 2458, 2694
TaiI ACGT 4 cut(s) 93, 490, 1269, 1858
TaqI TCGA 6 cut(s) 195, 502, 515, 1383, 2054, 2873
TatI WGTACW 2 cut(s) 1898, 2658
TauI GCSGC 3 cut(s) 209, 2759, 2891
TfiI GAWTC 6 cut(s) 41, 292, 578, 1456, 1963, 2392
Tru1I TTAA 9 cut(s) 255, 420, 735, 1110, 1128, 1278, 1614, 2165, 2376
Tru9I TTAA 9 cut(s) 255, 420, 735, 1110, 1128, 1278, 1614, 2165, 2376
TscAI CASTG 4 cut(s) 533, 868, 1732, 2079
TseFI GTSAC 5 cut(s) 91, 145, 476, 626, 1233
TseI GCWGC 7 cut(s) 209, 1180, 1924, 2138, 2258, 2794, 2914
Tsp45I GTSAC 5 cut(s) 91, 145, 476, 626, 1233
TspDTI ATGAA 7 cut(s) 470, 699, 801, 954, 1454, 2151, 2259
TspGWI ACGGA 2 cut(s) 936, 1338
TspRI CASTG 4 cut(s) 533, 868, 1732, 2079
Tth111I GACNNNGTC 2 cut(s) 52, 1401
Van91I CCANNNNNTGG 1 cut(s) 1028
VpaK11BI GGWCC 9 cut(s) 112, 914, 1040, 1067, 1166, 1403, 1430, 1892, 2225
XagI CCTNNNNNAGG 1 cut(s) 2550
XapI RAATTY 5 cut(s) 76, 770, 1147, 1730, 2328
XbaI TCTAGA 1 cut(s) 2099
XceI RCATGY 1 cut(s) 973
XcmI CCANNNNNNNNNTGG 1 cut(s) 1159
XhoI CTCGAG 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 2873
XmnI GAANNNNTTC 1 cut(s) 1734
ZraI GACGTC 1 cut(s) 91
ZrmI AGTACT 1 cut(s) 1900
Zsp2I ATGCAT 1 cut(s) 2353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.