pycom04g03870

Flowering time control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0002760
Physical Location & Seq
Reverse (-)
3473631 .. 3480141
6511 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g03870.1

Sequence Viewer

Length: 2334 bp
ATGGCGCCTGCGGCCAAACCTAACAAGCAAGGCACAGACGATTCCGAGACGCCGTCGAACAATCTCTGGGTTGGAAATTTGGCGAGCGACGTCACCGACGCCGATCTCATGGACCTGTTCGCCCAATTCGGTGCTTTGGATAGCGTCACCTCCTACTCGTCACGGAGCTACGCCTTCGTGTTCTTCAAGCGCGTGGAGGACGCTGCGGCGGCCAAGGAGGCATTGCAAGGTGCCCTTCTGCGCGGAAACCCTGTTAAGATTGAGTTTGCCCGACCGGCAAAACCTTGCAAGAATCTATGGGTAGGTGGAATTAGCCCATCTGTCTCAAAGGAAGAATTAGAAGAAGAGTTTTGTAAGTTTGGAAAAATGGAGGACTTCAAGTTCCTTAGAGACCGCAATACTGCATTCGTTGAATATTTTAAATTGGAGGATGCTTCCCAAGCAATGAGAAACATGAACGGGAAGAGATTGGGTGGTGACCAGATACGCGTTGATTATCATCGATCACAATACTCGAGAAGGGAACAGCCTGACTACAGAGATGGACAGTTTCTGGCCAGAAACATGGGGCCTGCTGATTCTCATAAAAGACAACAGTATTCTCAATCTTCCGGGGGACGAAAAGGTGACAGTCAACCCAGTAATGTTCTGTGGGTGGGCTATCCTCCTTCTGTGCAGATTGATGAACAAATGCTCCACAATGCCATGATTTTGTTTGGTGAGATTGAGAGAATTAAAAGTTTTCCTTCAAGACACTACTCCTTTGTCGAATTCAGGAGTGTGGATGAAGCTCGGCGGGCCAAGGAGGGCTTGCAAGGCAGGCTTTTCAATGATCCTCGGATTACAATTATGTTCTCAAGCAGTGGGCTGGCACCTGGGAAAGAATACCCTGGCCCTTATCCTGGAGGCCAAGGACCTAGATCAGATATGTTATTCAATGAACATCCTTTTCAGTCTTTACCGATGGACATGTTTGGTCATAACCGTCCAATGATGTCTAATAATTATCCTGGAGCACTACCACAAAATGGCATTCTTGGACCAAATGCTCCAATGAGGCCTTTAGGTCCTCAAGGTAGGTTTGACCCTCTGCTTTCAGGTCCAGAACTTAATGATTTGGCATCGCTTCATAATTATCAAGAAGGAAATTCCAAGAACCTGATGGGTCCCAACTGGAGGCAGCTGTCTCCTCCCACACCTGGGGCCGTGTCTTCTCCTGCGCCTGGTAGTAGGCCACCCACGAGACCTGCATCTAGTGCATGGGATGTGTTAGATGCTAACCAATTTCAAAAGGATGCTAAACGATCAAGAATAGATAGTCCTTTGTCCATGGACGACCCTCCATATCAGTTTAGAAACGTTGATGACCATGGATTAGGGTTCGACTCGTCTTACGGACTTGGTCCGGTAATTGATGGAGGTGCTTCCAGACCATCTATGAATGTCCAAGGGAAGAATCGCCTTAGTCCAGCTGGTGTAAGGGTCTCAGTTGGAGGGCCTCCTGAAAATGATTTTATATGGCGTGGAACTATTGCCAAGGGTGGAACACCTGTTTGCCATGCTCGCTGTGTCCCTATAGGAAAAGGAATACGAAATGAGCTTCCTGAAGTTGTTAATTGTTCGGCTAGAACTGGACTGGATATGCTCACAAAACACTATGCTGAGGCCATTGGATTTGATATCGTTTTCTTCTTGCCAGATAGTGAAGATGATTTTGCTTCCTACACTGAATTCCTACGTTATCTAGGTGCAAAAAACCGTGCTGGTGTTGCAAAGTTTGATGATGGGATGACCTTATTTTTGGTGCCTCCTTCAGATTTCTTGAAGAATGTTTTAAAAGTTGCAGGCCCTGAACGTCTGTATGGTGTGGTTCTCAAGTTTCCACCAGTTCCTAGTACTGCTTCTATGCACGAACAGATGCAGCCCATGCCACCTTCACAGTTTATAGAAAGACAGCAGATTCCGTCTTCACAAGTTGAGTATAGTGTAATCCCACCGAAGGAGGATCATATTTTGCCTATGGATTATAACAGGGTTTTGTATGAGGACTCAAAGCTTTCTGCAAAACCACTGTTTCCACCAACTGGCGAGTCCTCTAGAGTGCAACCGCAAGACTATGCTTCTAGTAATAGTACTGCAGTATCACAAGCTGGAGTTGCTTTAACTCCTGAACTTATTGCCACTCTGGCGACTTTACTGCCTGGAAATGCACAGCCGTCTGGTCCAGAAGGTGCTAGGGTACCAGTATCCTCAGCAGCTAGGCATTCATTTCCTGCTTTTGCACCTAGTGAAGTATCTTCTCCTGGATGGAAACAAGATCGAACAAATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0000785 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006325 GO:0006342 GO:0006355 GO:0006378 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009553 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0019219 GO:0019222 GO:0022414 GO:0031047 GO:0031048 GO:0031123 GO:0031124 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0034641 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
RRM_1 SPOC

Protein Analysis

778

Amino Acids

85.41

Weight (kDa)

6.4

Isoelectric Point (pI)

58.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 22 - 85 3e-13 RNA recognition motif
RRM_1 PF00076 99 - 163 3.7e-14 RNA recognition motif
RRM_1 PF00076 227 - 281 4e-07 RNA recognition motif
SPOC PF07744 501 - 626 4.9e-26 SPOC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2028
AatII GACGTC 1 cut(s) 93
Acc36I ACCTGC 1 cut(s) 1255
Acc65I GGTACC 1 cut(s) 2239
AccB1I GGYRCC 5 cut(s) 4, 230, 871, 1804, 2239
AccB7I CCANNNNNTGG 2 cut(s) 1028, 2084
AccII CGCG 3 cut(s) 192, 243, 489
AciI CCGC 7 cut(s) 11, 206, 209, 243, 394, 796, 2108
AclI AACGTT 1 cut(s) 1359
AclWI GGATC 2 cut(s) 827, 2013
AcoI YGGCCR 3 cut(s) 12, 210, 555
AcsI RAATTY 5 cut(s) 76, 770, 1147, 1730, 2326
AcuI CTGAAG 2 cut(s) 1626, 1797
AcyI GRCGYC 4 cut(s) 5, 50, 90, 99
AdeI CACNNNGTG 1 cut(s) 2288
AfaI GTAC 3 cut(s) 1897, 2134, 2241
AfiI CCNNNNNNNGG 8 cut(s) 902, 1028, 1176, 1199, 1200, 1223, 1999, 2084
AflIII ACRYGT 2 cut(s) 487, 969
AgsI TTSAA 8 cut(s) 187, 379, 413, 750, 829, 937, 1289, 1825
AjnI CCWGG 8 cut(s) 874, 889, 901, 1009, 1198, 1222, 2200, 2302
AloI GAACNNNNNNTCC 4 cut(s) 365, 397, 678, 710
AluBI AGCT 8 cut(s) 168, 791, 1183, 1472, 1600, 2056, 2150, 2258
AluI AGCT 8 cut(s) 168, 791, 1183, 1472, 1600, 2056, 2150, 2258
Alw21I GWGCWC 1 cut(s) 1018
Alw26I GTCTC 6 cut(s) 41, 328, 384, 1191, 1237, 1489
AlwI GGATC 2 cut(s) 827, 2013
AlwNI CAGNNNCTG 2 cut(s) 553, 1850
Ama87I CYCGRG 1 cut(s) 514
ApeKI GCWGC 4 cut(s) 203, 1180, 1921, 2255
ApoI RAATTY 5 cut(s) 76, 770, 1147, 1730, 2326
ArsI GACNNNNNNTTYG 2 cut(s) 1783, 1815
Asp718I GGTACC 1 cut(s) 2239
AspLEI GCGC 4 cut(s) 7, 192, 243, 1222
AsuC2I CCSGG 1 cut(s) 613
AsuHPI GGTGA 5 cut(s) 85, 139, 488, 638, 731
AvaI CYCGRG 1 cut(s) 514
AvaII GGWCC 8 cut(s) 112, 914, 1040, 1067, 1100, 1166, 1403, 2222
BaeGI GKGCMC 1 cut(s) 235
BaeI ACNNNNGTAYC 2 cut(s) 2124, 2157
BalI TGGCCA 1 cut(s) 557
BanI GGYRCC 5 cut(s) 4, 230, 871, 1804, 2239
BauI CACGAG 1 cut(s) 1240
BbsI GAAGAC 2 cut(s) 1203, 1959
Bbv12I GWGCWC 1 cut(s) 1018
BbvCI CCTCAGC 2 cut(s) 1662, 2251
BbvI GCAGC 4 cut(s) 190, 1192, 1933, 2267
BccI CCATC 8 cut(s) 325, 536, 958, 1156, 1409, 1441, 1778, 2301
BceAI ACGGC 3 cut(s) 37, 1190, 2200
BcgI CGANNNNNNTGC 2 cut(s) 2179, 2213
BciT130I CCWGG 8 cut(s) 876, 891, 903, 1011, 1200, 1224, 2202, 2304
BciVI GTATCC 1 cut(s) 2257
BcnI CCSGG 1 cut(s) 613
BcoDI GTCTC 6 cut(s) 41, 328, 384, 1191, 1237, 1489
BfmI CTRYAG 3 cut(s) 535, 1575, 2136
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 1255
BfuI GTATCC 1 cut(s) 2257
BglI GCCNNNNNGGC 3 cut(s) 218, 275, 2186
BisI GCNGC 7 cut(s) 12, 204, 207, 210, 1181, 1922, 2256
BlsI GCNGC 7 cut(s) 13, 205, 208, 211, 1182, 1923, 2257
BmcAI AGTACT 2 cut(s) 1897, 2134
Bme1390I CCNGG 9 cut(s) 613, 876, 891, 903, 1011, 1200, 1224, 2202, 2304
Bme18I GGWCC 8 cut(s) 112, 914, 1040, 1067, 1100, 1166, 1403, 2222
BmeT110I CYCGRG 1 cut(s) 514
BmiI GGNNCC 9 cut(s) 6, 232, 570, 873, 1167, 1168, 1204, 1806, 2241
BmrFI CCNGG 9 cut(s) 613, 876, 891, 903, 1011, 1200, 1224, 2202, 2304
BmrI ACTGGG 1 cut(s) 633
BmsI GCATC 6 cut(s) 421, 1130, 1259, 1264, 1285, 1908
BmuI ACTGGG 1 cut(s) 633
BpiI GAAGAC 2 cut(s) 1203, 1959
BpmI CTGGAG 4 cut(s) 924, 1032, 1195, 2172
Bpu10I CCTNAGC 2 cut(s) 1662, 2251
BpuEI CTTGAG 3 cut(s) 841, 1056, 1859
BpuMI CCSGG 1 cut(s) 613
Bsa29I ATCGAT 1 cut(s) 502
BsaBI GATNNNNATC 1 cut(s) 498
BsaHI GRCGYC 4 cut(s) 5, 50, 90, 99
BsaI GGTCTC 3 cut(s) 384, 1237, 1489
BsaWI WCCGGW 1 cut(s) 1405
BsaXI ACNNNNNCTCC 2 cut(s) 678, 708
Bsc4I CCNNNNNNNGG 8 cut(s) 902, 1028, 1176, 1199, 1200, 1223, 1999, 2084
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 7 cut(s) 639, 1178, 1636, 1641, 1886, 2089, 2243
Bse3DI GCAATG 2 cut(s) 221, 450
Bse8I GATNNNNATC 1 cut(s) 498
BseBI CCWGG 8 cut(s) 876, 891, 903, 1011, 1200, 1224, 2202, 2304
BseCI ATCGAT 1 cut(s) 502
BseGI GGATG 7 cut(s) 436, 790, 943, 1270, 1300, 1794, 2312
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 8 cut(s) 902, 1028, 1176, 1199, 1200, 1223, 1999, 2084
BseMI GCAATG 2 cut(s) 221, 450
BseMII CTCAG 3 cut(s) 1500, 1653, 2265
BseNI ACTGG 7 cut(s) 639, 1178, 1636, 1641, 1886, 2089, 2243
BseRI GAGGAG 1 cut(s) 1179
BseSI GKGCMC 1 cut(s) 235
BseXI GCAGC 4 cut(s) 190, 1192, 1933, 2267
BsgI GTGCAG 1 cut(s) 695
Bsh1236I CGCG 3 cut(s) 192, 243, 489
Bsh1285I CGRYCG 1 cut(s) 275
BshNI GGYRCC 5 cut(s) 4, 230, 871, 1804, 2239
BshVI ATCGAT 1 cut(s) 502
BsiEI CGRYCG 1 cut(s) 275
BsiHKAI GWGCWC 1 cut(s) 1018
BsiHKCI CYCGRG 1 cut(s) 514
BsiSI CCGG 3 cut(s) 275, 612, 1406
BslFI GGGAC 3 cut(s) 630, 1152, 1556
BslI CCNNNNNNNGG 8 cut(s) 902, 1028, 1176, 1199, 1200, 1223, 1999, 2084
BsmAI GTCTC 6 cut(s) 41, 328, 384, 1191, 1237, 1489
BsmBI CGTCTC 1 cut(s) 41
BsmFI GGGAC 3 cut(s) 630, 1152, 1556
BsmI GAATGC 3 cut(s) 404, 1032, 2263
Bso31I GGTCTC 3 cut(s) 384, 1237, 1489
BsoBI CYCGRG 1 cut(s) 514
Bsp1286I GDGCHC 2 cut(s) 235, 1018
Bsp143I GATC 7 cut(s) 103, 503, 832, 920, 1304, 2005, 2317
Bsp19I CCATGG 2 cut(s) 1329, 1369
BspACI CCGC 7 cut(s) 11, 206, 209, 243, 394, 796, 2108
BspCNI CTCAG 3 cut(s) 1499, 1654, 2264
BspDI ATCGAT 1 cut(s) 502
BspFNI CGCG 3 cut(s) 192, 243, 489
BspLI GGNNCC 9 cut(s) 6, 232, 570, 873, 1167, 1168, 1204, 1806, 2241
BspMAI CTGCAG 1 cut(s) 2140
BspMI ACCTGC 1 cut(s) 1255
BspPI GGATC 2 cut(s) 827, 2013
BspT107I GGYRCC 5 cut(s) 4, 230, 871, 1804, 2239
BspTNI GGTCTC 3 cut(s) 384, 1237, 1489
BsrDI GCAATG 2 cut(s) 221, 450
BsrFI RCCGGY 1 cut(s) 274
BsrI ACTGG 7 cut(s) 639, 1178, 1636, 1641, 1886, 2089, 2243
BssAI RCCGGY 1 cut(s) 274
BssMI GATC 7 cut(s) 103, 503, 832, 920, 1304, 2005, 2317
BssNI GRCGYC 4 cut(s) 5, 50, 90, 99
BssSI CACGAG 1 cut(s) 1240
BssT1I CCWWGG 7 cut(s) 213, 801, 910, 1329, 1369, 1447, 1536
Bst2BI CACGAG 1 cut(s) 1240
Bst2UI CCWGG 8 cut(s) 876, 891, 903, 1011, 1200, 1224, 2202, 2304
Bst4CI ACNGT 7 cut(s) 549, 597, 632, 986, 1760, 1941, 2073
Bst6I CTCTTC 2 cut(s) 339, 458
BstACI GRCGYC 4 cut(s) 5, 50, 90, 99
BstAPI GCANNNNNTGC 2 cut(s) 1256, 1927
BstC8I GCNNGC 9 cut(s) 9, 85, 573, 798, 812, 821, 870, 1564, 1846
BstDEI CTNAG 5 cut(s) 386, 1463, 1486, 1662, 2251
BstDSI CCRYGG 2 cut(s) 1329, 1369
BstEII GGTNACC 1 cut(s) 476
BstF5I GGATG 7 cut(s) 436, 790, 943, 1270, 1300, 1794, 2312
BstFNI CGCG 3 cut(s) 192, 243, 489
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 4 cut(s) 7, 192, 243, 1222
BstKTI GATC 7 cut(s) 106, 506, 835, 923, 1307, 2008, 2320
BstMAI GTCTC 6 cut(s) 41, 328, 384, 1191, 1237, 1489
BstMBI GATC 7 cut(s) 103, 503, 832, 920, 1304, 2005, 2317
BstMCI CGRYCG 1 cut(s) 275
BstNI CCWGG 8 cut(s) 876, 891, 903, 1011, 1200, 1224, 2202, 2304
BstNSI RCATGY 1 cut(s) 973
BstPI GGTNACC 1 cut(s) 476
BstSCI CCNGG 9 cut(s) 611, 874, 889, 901, 1009, 1198, 1222, 2200, 2302
BstSFI CTRYAG 3 cut(s) 535, 1575, 2136
BstSLI GKGCMC 1 cut(s) 235
BstUI CGCG 3 cut(s) 192, 243, 489
BstV1I GCAGC 4 cut(s) 190, 1192, 1933, 2267
BstV2I GAAGAC 2 cut(s) 1203, 1959
BstXI CCANNNNNNTGG 1 cut(s) 565
Bsu15I ATCGAT 1 cut(s) 502
BsuI GTATCC 1 cut(s) 2257
BsuTUI ATCGAT 1 cut(s) 502
BtgI CCRYGG 2 cut(s) 1329, 1369
BtgZI GCGATG 1 cut(s) 1107
BtsCI GGATG 7 cut(s) 436, 790, 943, 1270, 1300, 1794, 2312
BtsI GCAGTG 1 cut(s) 868
BtsIMutI CAGTG 3 cut(s) 868, 1725, 2069
BveI ACCTGC 1 cut(s) 1255
Cac8I GCNNGC 9 cut(s) 9, 85, 573, 798, 812, 821, 870, 1564, 1846
CaiI CAGNNNCTG 2 cut(s) 553, 1850
CfoI GCGC 4 cut(s) 7, 192, 243, 1222
Cfr10I RCCGGY 1 cut(s) 274
ClaI ATCGAT 1 cut(s) 502
CseI GACGC 4 cut(s) 58, 107, 133, 209
Csp6I GTAC 3 cut(s) 1896, 2133, 2240
CviQI GTAC 3 cut(s) 1896, 2133, 2240
DdeI CTNAG 5 cut(s) 386, 1463, 1486, 1662, 2251
DinI GGCGCC 1 cut(s) 6
DpnI GATC 7 cut(s) 105, 505, 834, 922, 1306, 2007, 2319
DpnII GATC 7 cut(s) 103, 503, 832, 920, 1304, 2005, 2317
DraI TTTAAA 2 cut(s) 421, 1836
DraIII CACNNNGTG 1 cut(s) 2288
EaeI YGGCCR 3 cut(s) 12, 210, 555
Eam1104I CTCTTC 2 cut(s) 339, 458
EarI CTCTTC 2 cut(s) 339, 458
Eco130I CCWWGG 7 cut(s) 213, 801, 910, 1329, 1369, 1447, 1536
Eco147I AGGCCT 1 cut(s) 1060
Eco31I GGTCTC 3 cut(s) 384, 1237, 1489
Eco32I GATATC 1 cut(s) 1681
Eco47I GGWCC 8 cut(s) 112, 914, 1040, 1067, 1100, 1166, 1403, 2222
Eco57I CTGAAG 2 cut(s) 1626, 1797
Eco88I CYCGRG 1 cut(s) 514
Eco91I GGTNACC 1 cut(s) 476
EcoO109I RGGNCCY 6 cut(s) 569, 914, 1067, 1166, 1496, 1847
EcoO65I GGTNACC 1 cut(s) 476
EcoRI GAATTC 2 cut(s) 770, 1730
EcoRII CCWGG 8 cut(s) 874, 889, 901, 1009, 1198, 1222, 2200, 2302
EcoRV GATATC 1 cut(s) 1681
EcoT14I CCWWGG 7 cut(s) 213, 801, 910, 1329, 1369, 1447, 1536
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 7 cut(s) 213, 801, 910, 1329, 1369, 1447, 1536
Esp3I CGTCTC 1 cut(s) 41
FalI AAGNNNNNCTT 8 cut(s) 219, 251, 730, 762, 794, 826, 807, 839
FaqI GGGAC 3 cut(s) 630, 1152, 1556
FauI CCCGC 1 cut(s) 789
Fnu4HI GCNGC 7 cut(s) 12, 204, 207, 210, 1181, 1922, 2256
FokI GGATG 7 cut(s) 443, 797, 930, 1277, 1307, 1801, 2319
Fsp4HI GCNGC 7 cut(s) 12, 204, 207, 210, 1181, 1922, 2256
GlaI GCGC 4 cut(s) 6, 191, 242, 1221
GluI GCNGC 7 cut(s) 12, 204, 207, 210, 1181, 1922, 2256
GsuI CTGGAG 4 cut(s) 924, 1032, 1195, 2172
HaeII RGCGCY 1 cut(s) 8
HapII CCGG 3 cut(s) 275, 612, 1406
HgaI GACGC 4 cut(s) 58, 107, 133, 209
HhaI GCGC 4 cut(s) 7, 192, 243, 1222
Hin1I GRCGYC 4 cut(s) 5, 50, 90, 99
Hin6I GCGC 4 cut(s) 5, 190, 241, 1220
HinP1I GCGC 4 cut(s) 5, 190, 241, 1220
HincII GTYRAC 1 cut(s) 635
HindII GTYRAC 1 cut(s) 635
HindIII AAGCTT 1 cut(s) 2054
HinfI GANTC 8 cut(s) 41, 292, 578, 1385, 1456, 1960, 2048, 2090
HpaII CCGG 3 cut(s) 275, 612, 1406
HphI GGTGA 5 cut(s) 85, 139, 488, 638, 731
Hpy166II GTNNAC 1 cut(s) 635
Hpy188I TCNGA 5 cut(s) 46, 840, 925, 1816, 2333
Hpy8I GTNNAC 1 cut(s) 635
Hpy99I CGWCG 3 cut(s) 58, 92, 101
HpyCH4III ACNGT 7 cut(s) 549, 597, 632, 986, 1760, 1941, 2073
HpyCH4IV ACGT 4 cut(s) 90, 1359, 1738, 1855
HpyF3I CTNAG 5 cut(s) 386, 1463, 1486, 1662, 2251
HpySE526I ACGT 4 cut(s) 90, 1359, 1738, 1855
Hsp92I GRCGYC 4 cut(s) 5, 50, 90, 99
HspAI GCGC 4 cut(s) 5, 190, 241, 1220
KasI GGCGCC 1 cut(s) 4
KflI GGGWCCC 1 cut(s) 1166
KpnI GGTACC 1 cut(s) 2243
Kzo9I GATC 7 cut(s) 103, 503, 832, 920, 1304, 2005, 2317
LmnI GCTCC 4 cut(s) 165, 699, 1013, 1054
Lsp1109I GCAGC 4 cut(s) 190, 1192, 1933, 2267
LweI GCATC 6 cut(s) 421, 1130, 1259, 1264, 1285, 1908
MaeII ACGT 4 cut(s) 90, 1359, 1738, 1855
MaeIII GTNAC 5 cut(s) 91, 145, 159, 476, 626
MalI GATC 7 cut(s) 105, 505, 834, 922, 1306, 2007, 2319
MboI GATC 7 cut(s) 103, 503, 832, 920, 1304, 2005, 2317
MhlI GDGCHC 2 cut(s) 235, 1018
MlsI TGGCCA 1 cut(s) 557
MluI ACGCGT 1 cut(s) 487
MluNI TGGCCA 1 cut(s) 557
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 3 cut(s) 1379, 2042, 2099
MmeI TCCRAC 2 cut(s) 52, 1471
Mox20I TGGCCA 1 cut(s) 557
MscI TGGCCA 1 cut(s) 557
MseI TTAA 7 cut(s) 255, 420, 735, 1110, 1614, 1835, 2162
Msp20I TGGCCA 1 cut(s) 557
MspA1I CMGCKG 2 cut(s) 1183, 1472
MspI CCGG 3 cut(s) 275, 612, 1406
MspR9I CCNGG 9 cut(s) 613, 876, 891, 903, 1011, 1200, 1224, 2202, 2304
Mva1269I GAATGC 3 cut(s) 404, 1032, 2263
MvaI CCWGG 8 cut(s) 876, 891, 903, 1011, 1200, 1224, 2202, 2304
MvnI CGCG 3 cut(s) 192, 243, 489
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 613
NcoI CCATGG 2 cut(s) 1329, 1369
NdeII GATC 7 cut(s) 103, 503, 832, 920, 1304, 2005, 2317
NlaIV GGNNCC 9 cut(s) 6, 232, 570, 873, 1167, 1168, 1204, 1806, 2241
NmeAIII GCCGAG 1 cut(s) 772
NmuCI GTSAC 5 cut(s) 91, 145, 159, 476, 626
NspI RCATGY 1 cut(s) 973
PaeR7I CTCGAG 1 cut(s) 514
PceI AGGCCT 1 cut(s) 1060
PciI ACATGT 1 cut(s) 969
PctI GAATGC 3 cut(s) 404, 1032, 2263
PfeI GAWTC 5 cut(s) 41, 292, 578, 1456, 1960
PflFI GACNNNGTC 2 cut(s) 52, 1401
PflMI CCANNNNNTGG 2 cut(s) 1028, 2084
PfoI TCCNGGA 3 cut(s) 901, 1009, 2302
PkrI GCNGC 7 cut(s) 13, 205, 208, 211, 1182, 1923, 2257
PleI GAGTC 3 cut(s) 1379, 2042, 2098
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 3 cut(s) 1379, 2042, 2098
PpuMI RGGWCCY 3 cut(s) 914, 1067, 1166
PscI ACATGT 1 cut(s) 969
PsiI TTATAA 1 cut(s) 2028
Psp1406I AACGTT 1 cut(s) 1359
Psp5II RGGWCCY 3 cut(s) 914, 1067, 1166
Psp6I CCWGG 8 cut(s) 874, 889, 901, 1009, 1198, 1222, 2200, 2302
PspEI GGTNACC 1 cut(s) 476
PspGI CCWGG 8 cut(s) 874, 889, 901, 1009, 1198, 1222, 2200, 2302
PspN4I GGNNCC 9 cut(s) 6, 232, 570, 873, 1167, 1168, 1204, 1806, 2241
PspPPI RGGWCCY 3 cut(s) 914, 1067, 1166
PstI CTGCAG 1 cut(s) 2140
PstNI CAGNNNCTG 2 cut(s) 553, 1850
PsyI GACNNNGTC 2 cut(s) 52, 1401
PvuII CAGCTG 2 cut(s) 1183, 1472
RsaI GTAC 3 cut(s) 1897, 2134, 2241
RsaNI GTAC 3 cut(s) 1896, 2133, 2240
SaqAI TTAA 7 cut(s) 255, 420, 735, 1110, 1614, 1835, 2162
SatI GCNGC 7 cut(s) 12, 204, 207, 210, 1181, 1922, 2256
Sau3AI GATC 7 cut(s) 103, 503, 832, 920, 1304, 2005, 2317
ScaI AGTACT 2 cut(s) 1897, 2134
SchI GAGTC 3 cut(s) 1379, 2042, 2099
ScrFI CCNGG 9 cut(s) 613, 876, 891, 903, 1011, 1200, 1224, 2202, 2304
SduI GDGCHC 2 cut(s) 235, 1018
SfaNI GCATC 6 cut(s) 421, 1130, 1259, 1264, 1285, 1908
SfcI CTRYAG 3 cut(s) 535, 1575, 2136
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 514
SinI GGWCC 8 cut(s) 112, 914, 1040, 1067, 1100, 1166, 1403, 2222
SlaI CTCGAG 1 cut(s) 514
SmlI CTYRAG 4 cut(s) 514, 856, 1071, 1874
SmoI CTYRAG 4 cut(s) 514, 856, 1071, 1874
SseBI AGGCCT 1 cut(s) 1060
SsiI CCGC 7 cut(s) 11, 206, 209, 243, 394, 796, 2108
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 416
StuI AGGCCT 1 cut(s) 1060
StyD4I CCNGG 9 cut(s) 611, 874, 889, 901, 1009, 1198, 1222, 2200, 2302
StyI CCWWGG 7 cut(s) 213, 801, 910, 1329, 1369, 1447, 1536
TaaI ACNGT 7 cut(s) 549, 597, 632, 986, 1760, 1941, 2073
TaiI ACGT 4 cut(s) 93, 1362, 1741, 1858
TaqI TCGA 6 cut(s) 56, 502, 515, 768, 1383, 2320
TatI WGTACW 2 cut(s) 1895, 2132
TauI GCSGC 3 cut(s) 14, 209, 212
TfiI GAWTC 5 cut(s) 41, 292, 578, 1456, 1960
Tru1I TTAA 7 cut(s) 255, 420, 735, 1110, 1614, 1835, 2162
Tru9I TTAA 7 cut(s) 255, 420, 735, 1110, 1614, 1835, 2162
TscAI CASTG 3 cut(s) 868, 1732, 2076
TseFI GTSAC 5 cut(s) 91, 145, 159, 476, 626
TseI GCWGC 4 cut(s) 203, 1180, 1921, 2255
Tsp45I GTSAC 5 cut(s) 91, 145, 159, 476, 626
TspDTI ATGAA 7 cut(s) 470, 699, 801, 954, 1118, 1454, 2256
TspGWI ACGGA 3 cut(s) 178, 1410, 1953
TspRI CASTG 3 cut(s) 868, 1732, 2076
Tth111I GACNNNGTC 2 cut(s) 52, 1401
Van91I CCANNNNNTGG 2 cut(s) 1028, 2084
VpaK11BI GGWCC 8 cut(s) 112, 914, 1040, 1067, 1100, 1166, 1403, 2222
XapI RAATTY 5 cut(s) 76, 770, 1147, 1730, 2326
XbaI TCTAGA 1 cut(s) 2096
XceI RCATGY 1 cut(s) 973
XcmI CCANNNNNNNNNTGG 1 cut(s) 1159
XhoI CTCGAG 1 cut(s) 514
ZraI GACGTC 1 cut(s) 91
ZrmI AGTACT 2 cut(s) 1897, 2134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.