MD13G1271200.v1.1

Flowering time control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0002760
Physical Location & Seq
Forward (+)
36101937 .. 36112234
10298 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1271200.v1.1.491

Sequence Viewer

Length: 2964 bp
ATGGCGCCTCCGACCAAACCCAACAAGCTAGCCACAGACGATTCGGAGACGCCGTCCAACAATCTCTGGGTTGGGAATTTGGCCAGCGACGTCACCGACTCGGATCTCATGGACCTTTTCGCCCAATTCGGTGCTTTGGATAGCGTCACCACCTACTCCTCCCGGAGCTACGCCTTCGTCTTCTTCAAGCGCGTCGAGGACTCCGCCGCTGCCAAGGAGGCATTGCAGGGTGCCCTTCTGCGCGGAAACCCTGTTAAGATTGAGTTTGCCCGACCGGCAAAACCTTGCAAGAATCTATGGGTAGGTGGAATTAGCCCATCTGTCTCAAAGGAAGAATTAGAAGAAGAGTTTTGTAAGTTTGGAAAAATGGAGGACTTCAAGTTCCTTAGAGACCGTAATACTGCATTTGTTGAATATTTTAAATTGGAGGATGCTTCTCAAGCAATGAGAAACATGAACGGGAAGAGATTGGCTGGTGACCAGATACGTGTTGATTATCTTCGATCGCAACCCTCGAGAAGAGAACAGTGGCCTGACTACAGAGACGGACAGTTTCCAGCCAGAAACACGGGGCCTGATTCTCACAAAAGACAACAGTATCCTCAATCTTCCGGGGGACGAAAAGGTGACAGCCAACCCAGTAATGTTCTTTGGGTTGGTTATCCTCCTTCTGTTCAGATTGATGAACAAATGCTCCACAATGCCATGATTTTGTTTGGTGAGATTGAGAGAATTAAAAGTTTTCCTTCAAGGCACTACTCCTTTGTTGAATTCAGGAGTGTAGATGAAGCTCGGCGGGCCAAGGAGGGCTTACAAGGCAGGCTTTTCAATGATCCTCGGATTACAATTATGTTCTCAAGCAGTGGGCTGGCACCTGGGAAAGATTACCCTGGCCCTTATCCTGGAGTCCAAGGACCTAGATCAGATATGTTATTCAATGAACATCCATTTCGGCCTTTACAGATGGACATGTTTGGTCATAACCGTCCAATGATGTCTAATAATTATCTTGGAGCTCTACCACAAAATGGCATTCTTGGACCAAATGCTCCAATGAGGCCTTTAGGTCCTCAAGGTAGGTTTGACCCTCTGCTTTCAGGCCCCGAACTTAATGATTTGGCATCACATAATAATTATCAAGATGGAAGTTCCAAGAACCTGATGGGTCTCAACTGGAGGCAGCTGTCTCCTCCCACACCTGGGGTCGTGTCTTCTCCTGCGCCTGGTATTAGGTCACACACGAGACCTGCATCTAGTGCATGGGACGTTTTGGATGTTAACCAATTTCAAAGGGATGCTAAACGGTCAAGGATAGATAGTCCTTTGTCCATAGACGACCCTCCATATCCGTTGAGAAAGATTGATGACCATGGATTAGGGTTCGACTCGTCTTACGGACTTGGTCCAGTAATTGATGGAGGTGCTTCTGGACCATCTATGAATGTCCAAGGGAAGAATCACCTTAGTCCAGCTGGTGTAAGGGTCTCAGTTGGAGGGCTTCCTGATAATGATTTTATATGGCGTGGAACTATTGCCAAGGGTGGAACACCTGTTTGCCATGCTCGCTGTGTCCCTATAGGAAAAGGAATAGGAAATGAGCTTCCTGGAGTTGTTAATTGTTCGGCTAGAACTGGACTGGATATGCTCACAAAACATTATGCTGAGGCCATTGGATTTGATATAGTATTCTTCTTGCCAGATAGTGAAGATGATTTTGCTTCCTACACTGAATTCCTTCGTTATCTAGGTGCAAAAAACCGTGCTGGTGTTGCAAAGTTTGATGATGGGATGACCTTATTTTTGGTGCCTCCTTCAGATTTCTTGAAGAATGTTTTGAAAGTTGCAGGCCCTGAACGTTTGTATGGTGTGGTTCTCAAGTTTCCACAACAAGGTCCTAGTACTGCCTCCATGCACGAACAGATGCAGCCTATGCCACCTTTACAGTTTATAGACAGACAGCAGATTCCTTCTTCACAAGTTGAGTATAGTGTAATCCCACCGAATGATGATCATATTTTACCTATGGATTATAACAGGGTTTTGCATGAGGGCTCGAAGCTTTCTGCAAAACCACTGTTTCCACCTACTAGCGAGTCCTCTAGAGTGCAACCACAAGACTATGCTTCTAGTAATAGTGCTGCAGTATCACAAGCTGGAGTTTCATTAACTCCTGAACTTATTGCCACTCTGGCCACTTTACTGCCTGGAAATGCACAGCCGTCTGGTCCAGAAGGTGCTAGGGTGCCAGTATCCTCAGCAGCTAGGCATTCATTTCCTGCTTTTGCACCAAGTAAAGTATCCTCTCCTGGATGGAAACAAGATCAACAAATTTCTGATCATACTGGTCATGCATTACAACAGTTAGGTAGTCAGTTTAATCCACACGAACAGAATCTTTTGCAGTACCAGCCTTATCCATCTGTTCCATACTCATCCAACCATTCTGCCCCACTGGCCCTTGGTATCAACCAAATCCCAGACTCTTCTACCAGTCAGCCCTTGCAGTCTGCAAATCCTTCTAGGCCTTTGAATAATTTTACAATCCCTTCTCAAGGTGGTGGACAAGTTACTGGATCCTCTTATCTCAGCCAGTATCTGGCTGAAGCTCCTCTTGGTACTCAGAAATCAGCACATGGAACTGGGTTGTACAATCCACCTGTTTCTCAGCAACATAATAATTCCATGGCCTTTTCTGATCAGACTTATGGTGCTAATTCGCAGTCTCAGACCATTCTGCCATTAGCGGCTGAAAAGGTAAACCCAGAATACCCAAATCAAGTGCAGCAACTTCAGTCTGCACTTATTGGGGCTGGTCAGAGCGCCCCAGATGGTGAGGCTGACAAAAATCATCGGTATCAGTCGACGCTCCAATTTGCAGCTAACCTCCTCCTCCAATTACAGCAGCAACAACAGCAGCAGCAAATGGGTAGTCAAGCAGGGCGAGGGTCTGGAAGTCAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000375 GO:0000377 GO:0000398 GO:0000785 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006325 GO:0006342 GO:0006355 GO:0006378 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008380 GO:0009553 GO:0009791 GO:0009889 GO:0009890 GO:0009892 GO:0009909 GO:0009911 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0019219 GO:0019222 GO:0022414 GO:0031047 GO:0031048 GO:0031123 GO:0031124 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0034641 GO:0040029 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043631 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048229 GO:0048518 GO:0048519 GO:0048523 GO:0048580 GO:0048582 GO:0048608 GO:0048731 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051094 GO:0051171 GO:0051172 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:2000026 GO:2000112 GO:2000113 GO:2000241 GO:2000243 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
RRM_1 SPOC

Protein Analysis

988

Amino Acids

107.75

Weight (kDa)

6.36

Isoelectric Point (pI)

57.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 22 - 85 2.2e-12 RNA recognition motif
RRM_1 PF00076 99 - 163 1.6e-13 RNA recognition motif
RRM_1 PF00076 227 - 281 5.4e-07 RNA recognition motif
SPOC PF07744 501 - 626 3.4e-25 SPOC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2031
AatII GACGTC 1 cut(s) 93
Acc36I ACCTGC 1 cut(s) 1255
AccB1I GGYRCC 5 cut(s) 4, 230, 871, 1804, 2242
AccB7I CCANNNNNTGG 2 cut(s) 1028, 2596
AccI GTMKAC 1 cut(s) 2861
AccII CGCG 2 cut(s) 192, 243
AciI CCGC 5 cut(s) 204, 207, 243, 796, 2744
AclI AACGTT 1 cut(s) 1855
AclWI GGATC 4 cut(s) 111, 827, 2568, 2581
AcoI YGGCCR 2 cut(s) 81, 2190
AcsI RAATTY 4 cut(s) 76, 770, 1730, 2328
AcuI CTGAAG 3 cut(s) 1797, 2622, 2774
AcyI GRCGYC 3 cut(s) 5, 50, 90
AfaI GTAC 4 cut(s) 1900, 2405, 2617, 2648
AfiI CCNNNNNNNGG 8 cut(s) 902, 1028, 1199, 1200, 1223, 1889, 2552, 2596
AflIII ACRYGT 2 cut(s) 487, 969
AjnI CCWGG 8 cut(s) 874, 889, 901, 1198, 1222, 1603, 2203, 2305
AjuI GAANNNNNNNTTGG 2 cut(s) 2595, 2627
AloI GAACNNNNNNTCC 4 cut(s) 365, 397, 678, 710
Alw21I GWGCWC 1 cut(s) 1018
Alw26I GTCTC 9 cut(s) 41, 328, 384, 537, 1172, 1191, 1237, 1489, 2727
AlwI GGATC 4 cut(s) 111, 827, 2568, 2581
AlwNI CAGNNNCTG 2 cut(s) 1850, 2596
Ama87I CYCGRG 1 cut(s) 514
ApoI RAATTY 4 cut(s) 76, 770, 1730, 2328
ArsI GACNNNNNNTTYG 4 cut(s) 1783, 1815, 2914, 2946
Asp700I GAANNNNTTC 1 cut(s) 1734
AspLEI GCGC 5 cut(s) 7, 192, 243, 1222, 2822
AsuC2I CCSGG 2 cut(s) 163, 613
AsuHPI GGTGA 7 cut(s) 85, 139, 488, 638, 731, 1451, 2843
AsuNHI GCTAGC 1 cut(s) 28
AvaI CYCGRG 1 cut(s) 514
AvaII GGWCC 8 cut(s) 112, 914, 1040, 1067, 1403, 1430, 1892, 2225
BaeGI GKGCMC 1 cut(s) 235
BaeI ACNNNNGTAYC 2 cut(s) 581, 614
BalI TGGCCA 2 cut(s) 83, 2192
BamHI GGATCC 1 cut(s) 2573
BanI GGYRCC 5 cut(s) 4, 230, 871, 1804, 2242
BanII GRGCYC 2 cut(s) 1018, 2054
BauI CACGAG 1 cut(s) 1240
BbsI GAAGAC 2 cut(s) 172, 1203
Bbv12I GWGCWC 1 cut(s) 1018
BbvCI CCTCAGC 2 cut(s) 1662, 2254
BceAI ACGGC 2 cut(s) 37, 2203
BciT130I CCWGG 8 cut(s) 876, 891, 903, 1200, 1224, 1605, 2205, 2307
BciVI GTATCC 3 cut(s) 609, 2260, 2308
BclI TGATCA 3 cut(s) 2008, 2335, 2695
BcnI CCSGG 2 cut(s) 163, 613
BcoDI GTCTC 9 cut(s) 41, 328, 384, 537, 1172, 1191, 1237, 1489, 2727
BfmI CTRYAG 3 cut(s) 538, 1575, 2139
BfoI RGCGCY 2 cut(s) 8, 2823
BfuAI ACCTGC 1 cut(s) 1255
BfuI GTATCC 3 cut(s) 609, 2260, 2308
BglI GCCNNNNNGGC 4 cut(s) 218, 275, 2189, 2453
BmcAI AGTACT 1 cut(s) 1900
Bme18I GGWCC 8 cut(s) 112, 914, 1040, 1067, 1403, 1430, 1892, 2225
BmeT110I CYCGRG 1 cut(s) 514
BmiI GGNNCC 8 cut(s) 6, 232, 573, 873, 1102, 1806, 2244, 2575
BmrI ACTGGG 2 cut(s) 633, 2649
BmsI GCATC 5 cut(s) 421, 1130, 1259, 1285, 1911
BmtI GCTAGC 1 cut(s) 32
BmuI ACTGGG 2 cut(s) 633, 2649
BpiI GAAGAC 2 cut(s) 172, 1203
BpmI CTGGAG 4 cut(s) 924, 1195, 1626, 2175
Bpu10I CCTNAGC 2 cut(s) 1662, 2254
BpuEI CTTGAG 5 cut(s) 423, 841, 1056, 1859, 2535
BpuMI CCSGG 2 cut(s) 163, 613
BsaAI YACGTR 1 cut(s) 488
BsaHI GRCGYC 3 cut(s) 5, 50, 90
BsaI GGTCTC 4 cut(s) 384, 1172, 1237, 1489
BsaXI ACNNNNNCTCC 4 cut(s) 38, 68, 678, 708
Bsc4I CCNNNNNNNGG 8 cut(s) 902, 1028, 1199, 1200, 1223, 1889, 2552, 2596
Bse118I RCCGGY 1 cut(s) 274
Bse3DI GCAATG 2 cut(s) 221, 450
BseBI CCWGG 8 cut(s) 876, 891, 903, 1200, 1224, 1605, 2205, 2307
BseGI GGATG 7 cut(s) 436, 943, 1279, 1300, 1794, 2315, 2432
BseLI CCNNNNNNNGG 8 cut(s) 902, 1028, 1199, 1200, 1223, 1889, 2552, 2596
BseMI GCAATG 2 cut(s) 221, 450
BseMII CTCAG 7 cut(s) 1500, 1653, 2268, 2599, 2633, 2678, 2738
BseRI GAGGAG 5 cut(s) 148, 1179, 2598, 2876, 2879
BseSI GKGCMC 1 cut(s) 235
BsgI GTGCAG 2 cut(s) 2781, 2801
Bsh1236I CGCG 2 cut(s) 192, 243
Bsh1285I CGRYCG 2 cut(s) 275, 506
BshNI GGYRCC 5 cut(s) 4, 230, 871, 1804, 2242
BsiEI CGRYCG 2 cut(s) 275, 506
BsiHKAI GWGCWC 1 cut(s) 1018
BsiHKCI CYCGRG 1 cut(s) 514
BsiSI CCGG 3 cut(s) 163, 275, 612
BslFI GGGAC 3 cut(s) 630, 1277, 1556
BslI CCNNNNNNNGG 8 cut(s) 902, 1028, 1199, 1200, 1223, 1889, 2552, 2596
BsmAI GTCTC 9 cut(s) 41, 328, 384, 537, 1172, 1191, 1237, 1489, 2727
BsmBI CGTCTC 2 cut(s) 41, 537
BsmFI GGGAC 3 cut(s) 630, 1277, 1556
BsmI GAATGC 2 cut(s) 1032, 2266
Bso31I GGTCTC 4 cut(s) 384, 1172, 1237, 1489
BsoBI CYCGRG 1 cut(s) 514
Bsp1286I GDGCHC 3 cut(s) 235, 1018, 2054
Bsp1407I TGTACA 1 cut(s) 2646
Bsp143I GATC 9 cut(s) 103, 503, 832, 920, 2008, 2320, 2335, 2573, 2695
Bsp19I CCATGG 2 cut(s) 1369, 2682
BspACI CCGC 5 cut(s) 204, 207, 243, 796, 2744
BspCNI CTCAG 7 cut(s) 1499, 1654, 2267, 2598, 2632, 2677, 2737
BspFNI CGCG 2 cut(s) 192, 243
BspLI GGNNCC 8 cut(s) 6, 232, 573, 873, 1102, 1806, 2244, 2575
BspMAI CTGCAG 1 cut(s) 2143
BspMI ACCTGC 1 cut(s) 1255
BspOI GCTAGC 1 cut(s) 32
BspPI GGATC 4 cut(s) 111, 827, 2568, 2581
BspT107I GGYRCC 5 cut(s) 4, 230, 871, 1804, 2242
BspTNI GGTCTC 4 cut(s) 384, 1172, 1237, 1489
BsrDI GCAATG 2 cut(s) 221, 450
BsrFI RCCGGY 1 cut(s) 274
BsrGI TGTACA 1 cut(s) 2646
BssAI RCCGGY 1 cut(s) 274
BssMI GATC 9 cut(s) 103, 503, 832, 920, 2008, 2320, 2335, 2573, 2695
BssNI GRCGYC 3 cut(s) 5, 50, 90
BssSI CACGAG 1 cut(s) 1240
BssT1I CCWWGG 8 cut(s) 213, 801, 910, 1369, 1447, 1536, 2458, 2682
Bst2BI CACGAG 1 cut(s) 1240
Bst2UI CCWGG 8 cut(s) 876, 891, 903, 1200, 1224, 1605, 2205, 2307
Bst6I CTCTTC 4 cut(s) 339, 458, 514, 2488
BstACI GRCGYC 3 cut(s) 5, 50, 90
BstAPI GCANNNNNTGC 2 cut(s) 1256, 1930
BstAUI TGTACA 1 cut(s) 2646
BstBAI YACGTR 1 cut(s) 488
BstC8I GCNNGC 7 cut(s) 30, 85, 798, 821, 870, 1564, 1846
BstDEI CTNAG 9 cut(s) 386, 1463, 1486, 1662, 2254, 2585, 2619, 2664, 2724
BstDSI CCRYGG 2 cut(s) 1369, 2682
BstEII GGTNACC 1 cut(s) 476
BstENI CCTNNNNNAGG 1 cut(s) 2550
BstF5I GGATG 7 cut(s) 436, 943, 1279, 1300, 1794, 2315, 2432
BstFNI CGCG 2 cut(s) 192, 243
BstH2I RGCGCY 2 cut(s) 8, 2823
BstHHI GCGC 5 cut(s) 7, 192, 243, 1222, 2822
BstKTI GATC 9 cut(s) 106, 506, 835, 923, 2011, 2323, 2338, 2576, 2698
BstMAI GTCTC 9 cut(s) 41, 328, 384, 537, 1172, 1191, 1237, 1489, 2727
BstMBI GATC 9 cut(s) 103, 503, 832, 920, 2008, 2320, 2335, 2573, 2695
BstMCI CGRYCG 2 cut(s) 275, 506
BstNI CCWGG 8 cut(s) 876, 891, 903, 1200, 1224, 1605, 2205, 2307
BstNSI RCATGY 1 cut(s) 973
BstPI GGTNACC 1 cut(s) 476
BstSFI CTRYAG 3 cut(s) 538, 1575, 2139
BstSLI GKGCMC 1 cut(s) 235
BstUI CGCG 2 cut(s) 192, 243
BstV2I GAAGAC 2 cut(s) 172, 1203
BstX2I RGATCY 2 cut(s) 103, 2573
BstYI RGATCY 2 cut(s) 103, 2573
BsuI GTATCC 3 cut(s) 609, 2260, 2308
BtgI CCRYGG 2 cut(s) 1369, 2682
BtsCI GGATG 7 cut(s) 436, 943, 1279, 1300, 1794, 2315, 2432
BtsI GCAGTG 1 cut(s) 868
BtsIMutI CAGTG 5 cut(s) 533, 868, 1725, 2072, 2450
BveI ACCTGC 1 cut(s) 1255
Cac8I GCNNGC 7 cut(s) 30, 85, 798, 821, 870, 1564, 1846
CaiI CAGNNNCTG 2 cut(s) 1850, 2596
CfoI GCGC 5 cut(s) 7, 192, 243, 1222, 2822
Cfr10I RCCGGY 1 cut(s) 274
CseI GACGC 4 cut(s) 58, 133, 181, 2872
Csp6I GTAC 4 cut(s) 1899, 2404, 2616, 2647
CviQI GTAC 4 cut(s) 1899, 2404, 2616, 2647
DdeI CTNAG 9 cut(s) 386, 1463, 1486, 1662, 2254, 2585, 2619, 2664, 2724
DinI GGCGCC 1 cut(s) 6
DpnI GATC 9 cut(s) 105, 505, 834, 922, 2010, 2322, 2337, 2575, 2697
DpnII GATC 9 cut(s) 103, 503, 832, 920, 2008, 2320, 2335, 2573, 2695
DraI TTTAAA 1 cut(s) 421
EaeI YGGCCR 2 cut(s) 81, 2190
Eam1104I CTCTTC 4 cut(s) 339, 458, 514, 2488
EarI CTCTTC 4 cut(s) 339, 458, 514, 2488
EciI GGCGGA 1 cut(s) 193
Ecl136II GAGCTC 1 cut(s) 1016
Eco130I CCWWGG 8 cut(s) 213, 801, 910, 1369, 1447, 1536, 2458, 2682
Eco147I AGGCCT 2 cut(s) 1060, 2524
Eco24I GRGCYC 2 cut(s) 1018, 2054
Eco31I GGTCTC 4 cut(s) 384, 1172, 1237, 1489
Eco47I GGWCC 8 cut(s) 112, 914, 1040, 1067, 1403, 1430, 1892, 2225
Eco53kI GAGCTC 1 cut(s) 1016
Eco57I CTGAAG 3 cut(s) 1797, 2622, 2774
Eco88I CYCGRG 1 cut(s) 514
Eco91I GGTNACC 1 cut(s) 476
EcoICRI GAGCTC 1 cut(s) 1016
EcoNI CCTNNNNNAGG 1 cut(s) 2550
EcoO109I RGGNCCY 6 cut(s) 572, 914, 1067, 1100, 1847, 1892
EcoO65I GGTNACC 1 cut(s) 476
EcoRI GAATTC 2 cut(s) 770, 1730
EcoRII CCWGG 8 cut(s) 874, 889, 901, 1198, 1222, 1603, 2203, 2305
EcoT14I CCWWGG 8 cut(s) 213, 801, 910, 1369, 1447, 1536, 2458, 2682
EcoT22I ATGCAT 1 cut(s) 2353
EcoT38I GRGCYC 2 cut(s) 1018, 2054
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 8 cut(s) 213, 801, 910, 1369, 1447, 1536, 2458, 2682
Esp3I CGTCTC 2 cut(s) 41, 537
FalI AAGNNNNNCTT 8 cut(s) 730, 762, 794, 826, 807, 839, 2595, 2627
FaqI GGGAC 3 cut(s) 630, 1277, 1556
FauI CCCGC 1 cut(s) 789
FbaI TGATCA 3 cut(s) 2008, 2335, 2695
FblI GTMKAC 1 cut(s) 2861
FokI GGATG 7 cut(s) 443, 930, 1286, 1307, 1801, 2322, 2419
FriOI GRGCYC 2 cut(s) 1018, 2054
GlaI GCGC 5 cut(s) 6, 191, 242, 1221, 2821
GsuI CTGGAG 4 cut(s) 924, 1195, 1626, 2175
HaeII RGCGCY 2 cut(s) 8, 2823
HapII CCGG 3 cut(s) 163, 275, 612
HgaI GACGC 4 cut(s) 58, 133, 181, 2872
HhaI GCGC 5 cut(s) 7, 192, 243, 1222, 2822
Hin1I GRCGYC 3 cut(s) 5, 50, 90
Hin6I GCGC 5 cut(s) 5, 190, 241, 1220, 2820
HinP1I GCGC 5 cut(s) 5, 190, 241, 1220, 2820
HincII GTYRAC 3 cut(s) 1279, 2862, 2957
HindII GTYRAC 3 cut(s) 1279, 2862, 2957
HindIII AAGCTT 1 cut(s) 2057
HpaI GTTAAC 1 cut(s) 1279
HpaII CCGG 3 cut(s) 163, 275, 612
HphI GGTGA 7 cut(s) 85, 139, 488, 638, 731, 1451, 2843
Hpy166II GTNNAC 5 cut(s) 1279, 2561, 2758, 2862, 2957
Hpy8I GTNNAC 5 cut(s) 1279, 2561, 2758, 2862, 2957
Hpy99I CGWCG 3 cut(s) 92, 197, 2866
HpyCH4IV ACGT 4 cut(s) 90, 487, 1266, 1855
HpyF3I CTNAG 9 cut(s) 386, 1463, 1486, 1662, 2254, 2585, 2619, 2664, 2724
HpySE526I ACGT 4 cut(s) 90, 487, 1266, 1855
Hsp92I GRCGYC 3 cut(s) 5, 50, 90
HspAI GCGC 5 cut(s) 5, 190, 241, 1220, 2820
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 3 cut(s) 2008, 2335, 2695
KspAI GTTAAC 1 cut(s) 1279
Kzo9I GATC 9 cut(s) 103, 503, 832, 920, 2008, 2320, 2335, 2573, 2695
LmnI GCTCC 6 cut(s) 165, 699, 1013, 1054, 2611, 2871
LweI GCATC 5 cut(s) 421, 1130, 1259, 1285, 1911
MaeII ACGT 4 cut(s) 90, 487, 1266, 1855
MaeIII GTNAC 6 cut(s) 91, 145, 476, 626, 1233, 2566
MalI GATC 9 cut(s) 105, 505, 834, 922, 2010, 2322, 2337, 2575, 2697
MboI GATC 9 cut(s) 103, 503, 832, 920, 2008, 2320, 2335, 2573, 2695
MflI RGATCY 2 cut(s) 103, 2573
MhlI GDGCHC 3 cut(s) 235, 1018, 2054
MlsI TGGCCA 2 cut(s) 83, 2192
MluNI TGGCCA 2 cut(s) 83, 2192
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 6 cut(s) 92, 194, 915, 1379, 2102, 2474
MmeI TCCRAC 4 cut(s) 35, 81, 1471, 2460
Mox20I TGGCCA 2 cut(s) 83, 2192
Mph1103I ATGCAT 1 cut(s) 2353
MroXI GAANNNNTTC 1 cut(s) 1734
MscI TGGCCA 2 cut(s) 83, 2192
MseI TTAA 8 cut(s) 255, 420, 735, 1110, 1278, 1614, 2165, 2376
Msp20I TGGCCA 2 cut(s) 83, 2192
MspA1I CMGCKG 3 cut(s) 209, 1183, 1472
MspI CCGG 3 cut(s) 163, 275, 612
Mva1269I GAATGC 2 cut(s) 1032, 2266
MvaI CCWGG 8 cut(s) 876, 891, 903, 1200, 1224, 1605, 2205, 2307
MvnI CGCG 2 cut(s) 192, 243
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 2 cut(s) 163, 613
NcoI CCATGG 2 cut(s) 1369, 2682
NdeII GATC 9 cut(s) 103, 503, 832, 920, 2008, 2320, 2335, 2573, 2695
NheI GCTAGC 1 cut(s) 28
NlaIV GGNNCC 8 cut(s) 6, 232, 573, 873, 1102, 1806, 2244, 2575
NmeAIII GCCGAG 1 cut(s) 772
NmuCI GTSAC 5 cut(s) 91, 145, 476, 626, 1233
NsiI ATGCAT 1 cut(s) 2353
NspI RCATGY 1 cut(s) 973
PaeR7I CTCGAG 1 cut(s) 514
PceI AGGCCT 2 cut(s) 1060, 2524
PciI ACATGT 1 cut(s) 969
PcsI WCGNNNNNNNCGW 2 cut(s) 50, 512
PctI GAATGC 2 cut(s) 1032, 2266
PdmI GAANNNNTTC 1 cut(s) 1734
PfeI GAWTC 6 cut(s) 41, 292, 578, 1456, 1963, 2392
PflFI GACNNNGTC 2 cut(s) 52, 1401
PflMI CCANNNNNTGG 2 cut(s) 1028, 2596
PfoI TCCNGGA 4 cut(s) 161, 901, 1603, 2305
Ple19I CGATCG 1 cut(s) 506
PleI GAGTC 6 cut(s) 92, 194, 914, 1379, 2101, 2474
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 6 cut(s) 92, 194, 914, 1379, 2101, 2474
Ppu21I YACGTR 1 cut(s) 488
PpuMI RGGWCCY 3 cut(s) 914, 1067, 1892
PscI ACATGT 1 cut(s) 969
PsiI TTATAA 1 cut(s) 2031
Psp124BI GAGCTC 1 cut(s) 1018
Psp1406I AACGTT 1 cut(s) 1855
Psp5II RGGWCCY 3 cut(s) 914, 1067, 1892
Psp6I CCWGG 8 cut(s) 874, 889, 901, 1198, 1222, 1603, 2203, 2305
PspEI GGTNACC 1 cut(s) 476
PspGI CCWGG 8 cut(s) 874, 889, 901, 1198, 1222, 1603, 2203, 2305
PspN4I GGNNCC 8 cut(s) 6, 232, 573, 873, 1102, 1806, 2244, 2575
PspPPI RGGWCCY 3 cut(s) 914, 1067, 1892
PstI CTGCAG 1 cut(s) 2143
PstNI CAGNNNCTG 2 cut(s) 1850, 2596
PsuI RGATCY 2 cut(s) 103, 2573
PsyI GACNNNGTC 2 cut(s) 52, 1401
PvuI CGATCG 1 cut(s) 506
PvuII CAGCTG 2 cut(s) 1183, 1472
RsaI GTAC 4 cut(s) 1900, 2405, 2617, 2648
RsaNI GTAC 4 cut(s) 1899, 2404, 2616, 2647
SacI GAGCTC 1 cut(s) 1018
SalI GTCGAC 1 cut(s) 2860
SaqAI TTAA 8 cut(s) 255, 420, 735, 1110, 1278, 1614, 2165, 2376
Sau3AI GATC 9 cut(s) 103, 503, 832, 920, 2008, 2320, 2335, 2573, 2695
ScaI AGTACT 1 cut(s) 1900
SchI GAGTC 6 cut(s) 92, 194, 915, 1379, 2102, 2474
SduI GDGCHC 3 cut(s) 235, 1018, 2054
SfaNI GCATC 5 cut(s) 421, 1130, 1259, 1285, 1911
SfcI CTRYAG 3 cut(s) 538, 1575, 2139
SfoI GGCGCC 1 cut(s) 6
Sfr274I CTCGAG 1 cut(s) 514
SinI GGWCC 8 cut(s) 112, 914, 1040, 1067, 1403, 1430, 1892, 2225
SlaI CTCGAG 1 cut(s) 514
SmlI CTYRAG 6 cut(s) 438, 514, 856, 1071, 1874, 2550
SmoI CTYRAG 6 cut(s) 438, 514, 856, 1071, 1874, 2550
SseBI AGGCCT 2 cut(s) 1060, 2524
SsiI CCGC 5 cut(s) 204, 207, 243, 796, 2744
SspDI GGCGCC 1 cut(s) 4
SspI AATATT 1 cut(s) 416
SstI GAGCTC 1 cut(s) 1018
StuI AGGCCT 2 cut(s) 1060, 2524
StyI CCWWGG 8 cut(s) 213, 801, 910, 1369, 1447, 1536, 2458, 2682
TaiI ACGT 4 cut(s) 93, 490, 1269, 1858
TaqI TCGA 6 cut(s) 195, 502, 515, 1383, 2054, 2861
TatI WGTACW 2 cut(s) 1898, 2646
TauI GCSGC 2 cut(s) 209, 2747
TfiI GAWTC 6 cut(s) 41, 292, 578, 1456, 1963, 2392
Tru1I TTAA 8 cut(s) 255, 420, 735, 1110, 1278, 1614, 2165, 2376
Tru9I TTAA 8 cut(s) 255, 420, 735, 1110, 1278, 1614, 2165, 2376
TscAI CASTG 5 cut(s) 533, 868, 1732, 2079, 2457
TseFI GTSAC 5 cut(s) 91, 145, 476, 626, 1233
Tsp45I GTSAC 5 cut(s) 91, 145, 476, 626, 1233
TspDTI ATGAA 7 cut(s) 470, 699, 801, 954, 1454, 2151, 2259
TspGWI ACGGA 3 cut(s) 561, 1338, 1410
TspRI CASTG 5 cut(s) 533, 868, 1732, 2079, 2457
Tth111I GACNNNGTC 2 cut(s) 52, 1401
Van91I CCANNNNNTGG 2 cut(s) 1028, 2596
VpaK11BI GGWCC 8 cut(s) 112, 914, 1040, 1067, 1403, 1430, 1892, 2225
XagI CCTNNNNNAGG 1 cut(s) 2550
XapI RAATTY 4 cut(s) 76, 770, 1730, 2328
XbaI TCTAGA 1 cut(s) 2099
XceI RCATGY 1 cut(s) 973
XcmI CCANNNNNNNNNTGG 1 cut(s) 1159
XhoI CTCGAG 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 2861
XmnI GAANNNNTTC 1 cut(s) 1734
ZraI GACGTC 1 cut(s) 91
ZrmI AGTACT 1 cut(s) 1900
Zsp2I ATGCAT 1 cut(s) 2353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.