MD04G1052500.v1.1

CCR4-NOT transcription complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0006134
Physical Location & Seq
Forward (+)
6186552 .. 6193941
7390 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1052500.v1.1.491

Sequence Viewer

Length: 1311 bp
ATGAAGCTGGGGTTTGAAGAATCGAAGGTGGTGTTTGGACTGCTGAGAGCAGAGCAGCGCCCGATCGAAGAGATCGTCTCTGAGTTCAACTCCACATTCTCTCATGGCCGCCAATTCACTCCCTGCTTCTCTCTCTCCATTCTTTTACAGGACAAGAAGATGCTGAGCTCCACTCAGCGTTTGATTGCCTTCGCTATTCTTCAGCAGGCCTATTCATCCCAGAAACCTTCTGCAAATCCGTTCATTAATTTACTTATAAACGCTGCATGTGATGTCGAAGCCGAAAAGTATGAGAGGGCACTTGTTCTGCAGCTATTACAGCTGTTTGGCTCTGATGGCTCTAGTGATGGCAAAGAGATTCTTAAACAGTCTGCTGCAGAGTACATCAAAAGTTTTGATCCTTCAGTACATGCATTTCCGCAACATGAACAGTTGCAGCAGCAGTACGCTGATAAAGTCCATCCAGAACGATTTATTTCTCCATTCAAGGATGCTTCCGTCAGAAATGTGGTTGCAGACCCTGATGTTCCTCGTGGTTGTGATGTTAACTCATTAGAGTTTGATATACAAACTGGAGCAAAACCTAAAATTGGATCTGGAGATAGAGATGAGACGGTGCTTGGCTTGCTATCCAATTTAGCGCTGGAAGGGTTAGGTCCTCACTGGAACAGGCCTATTCCACCTAGGCTCCCAGTTCAGAATGATGAACTAGTGTGGCTTAACCCTGTGGACAATCATGAACTTTTATGGGATGATGGAATGTGTATTGATACTAGCAGAGGGGCAGCTGTCAGGTACTTGATTGCAAAAGCTTTAAAGGGACCACTTGCGCCTGCACAACAAGAGCAAGTCTTGTTGGAGCTGGCAAATGACCCAAAACTTGTATATCACTGTGGGCTGACACCAAGAAAGCTACCGGAATTGGTGGAAAATAATCCCGTTATTGCAGTTGAAGTCCTCACCAAGCTGATAAAATCCCCTGAAATTGCAGGATACTTTACAGTGCTTGTCAATATGGACATGAGTCTGCACTCAATGGAAGTGGTGAACAGGCTTACTACAGCAGTTGAACTTCCTTCCGGGTTCATACACATGTACATAACAAATTGTATATCATCTTGTGAGAACATCAAGGATAAATACATGCAGAACAGACTTGTGAGACTTGTTTGCGTGTTTCTTCAGAGCCTTATCCGAAACGACATTATCAATGTTAAGGATCTTTTCATCGAAGTCCAAGCTTTTTGCATAGAGTTCTCGCGGATTAGGGAAGCAGCGGCACTGTTTAGGCTCCTAAAGTCCTTGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2363

Protein Analysis

437

Amino Acids

48.95

Weight (kDa)

5.52

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CNOT11 PF10155 309 - 433 5.2e-53 CCR4-NOT transcription complex subunit 11
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 257
AccII CGCG 1 cut(s) 1261
AciI CCGC 4 cut(s) 109, 419, 1261, 1277
AclWI GGATC 3 cut(s) 392, 601, 1227
AcoI YGGCCR 1 cut(s) 106
AcuI CTGAAG 3 cut(s) 185, 387, 1166
AfaI GTAC 5 cut(s) 383, 408, 446, 797, 1097
AfeI AGCGCT 1 cut(s) 642
AfiI CCNNNNNNNGG 1 cut(s) 590
AflIII ACRYGT 1 cut(s) 1092
AgsI TTSAA 5 cut(s) 17, 88, 487, 953, 1070
AhlI ACTAGT 1 cut(s) 709
Alw21I GWGCWC 1 cut(s) 170
Alw26I GTCTC 3 cut(s) 82, 605, 1156
AlwI GGATC 3 cut(s) 392, 601, 1227
AlwNI CAGNNNCTG 1 cut(s) 521
Aor51HI AGCGCT 1 cut(s) 642
AoxI GGCC 3 cut(s) 106, 207, 671
ApeKI GCWGC 8 cut(s) 55, 263, 310, 374, 436, 439, 785, 1274
ArsI GACNNNNNNTTYG 2 cut(s) 60, 92
AseI ATTAAT 1 cut(s) 246
AspA2I CCTAGG 1 cut(s) 683
AspLEI GCGC 3 cut(s) 60, 643, 832
AspS9I GGNCC 2 cut(s) 656, 821
AsuC2I CCSGG 1 cut(s) 1081
AsuHPI GGTGA 2 cut(s) 952, 1057
AvaII GGWCC 2 cut(s) 656, 821
AvrII CCTAGG 1 cut(s) 683
BaeGI GKGCMC 1 cut(s) 301
BanII GRGCYC 1 cut(s) 170
BauI CACGAG 1 cut(s) 531
Bbv12I GWGCWC 1 cut(s) 170
BbvI GCAGC 8 cut(s) 67, 250, 322, 361, 448, 451, 797, 1286
BccI CCATC 4 cut(s) 329, 341, 468, 749
BciVI GTATCC 1 cut(s) 986
BcnI CCSGG 1 cut(s) 1081
BcoDI GTCTC 3 cut(s) 82, 605, 1156
BcuI ACTAGT 1 cut(s) 709
BfaI CTAG 4 cut(s) 342, 684, 710, 774
BfmI CTRYAG 3 cut(s) 308, 375, 1059
BfoI RGCGCY 2 cut(s) 61, 644
BfuI GTATCC 1 cut(s) 986
BlnI CCTAGG 1 cut(s) 683
BlpI GCTNAGC 1 cut(s) 164
Bme1390I CCNGG 1 cut(s) 1081
Bme18I GGWCC 2 cut(s) 656, 821
BmgT120I GGNCC 2 cut(s) 656, 821
BmiI GGNNCC 3 cut(s) 689, 822, 1292
BmrFI CCNGG 1 cut(s) 1081
BmrI ACTGGG 1 cut(s) 686
BmsI GCATC 2 cut(s) 150, 481
BmuI ACTGGG 1 cut(s) 686
BoxI GACNNNNGTC 1 cut(s) 1023
BplI GAGNNNNNCTC 6 cut(s) 62, 94, 74, 106, 157, 189
BpmI CTGGAG 2 cut(s) 594, 618
Bpu1102I GCTNAGC 1 cut(s) 164
BpuMI CCSGG 1 cut(s) 1081
BsaJI CCNNGG 2 cut(s) 683, 1302
BsaWI WCCGGW 1 cut(s) 916
BsaXI ACNNNNNCTCC 2 cut(s) 672, 702
Bsc4I CCNNNNNNNGG 1 cut(s) 590
Bse1I ACTGG 3 cut(s) 577, 668, 692
BseDI CCNNGG 2 cut(s) 683, 1302
BseGI GGATG 4 cut(s) 215, 460, 496, 757
BseLI CCNNNNNNNGG 1 cut(s) 590
BseMII CTCAG 4 cut(s) 35, 72, 155, 188
BseNI ACTGG 3 cut(s) 577, 668, 692
BseSI GKGCMC 1 cut(s) 301
BseXI GCAGC 8 cut(s) 67, 250, 322, 361, 448, 451, 797, 1286
BseYI CCCAGC 1 cut(s) 7
BsgI GTGCAG 2 cut(s) 819, 1013
Bsh1236I CGCG 1 cut(s) 1261
Bsh1285I CGRYCG 1 cut(s) 66
BshFI GGCC 3 cut(s) 108, 209, 673
BsiEI CGRYCG 1 cut(s) 66
BsiHKAI GWGCWC 1 cut(s) 170
BsiSI CCGG 2 cut(s) 917, 1080
BslFI GGGAC 1 cut(s) 834
BslI CCNNNNNNNGG 1 cut(s) 590
BsmAI GTCTC 3 cut(s) 82, 605, 1156
BsmBI CGTCTC 2 cut(s) 82, 605
BsmFI GGGAC 1 cut(s) 834
BsnI GGCC 3 cut(s) 108, 209, 673
Bsp1286I GDGCHC 2 cut(s) 170, 301
Bsp1407I TGTACA 1 cut(s) 1095
Bsp143I GATC 5 cut(s) 63, 72, 397, 593, 1219
Bsp1720I GCTNAGC 1 cut(s) 164
BspACI CCGC 4 cut(s) 109, 419, 1261, 1277
BspANI GGCC 3 cut(s) 108, 209, 673
BspCNI CTCAG 4 cut(s) 36, 73, 156, 187
BspFNI CGCG 1 cut(s) 1261
BspHI TCATGA 1 cut(s) 736
BspLI GGNNCC 3 cut(s) 689, 822, 1292
BspMAI CTGCAG 2 cut(s) 312, 379
BspPI GGATC 3 cut(s) 392, 601, 1227
BsrGI TGTACA 1 cut(s) 1095
BsrI ACTGG 3 cut(s) 577, 668, 692
BssECI CCNNGG 2 cut(s) 683, 1302
BssMI GATC 5 cut(s) 63, 72, 397, 593, 1219
BssSI CACGAG 1 cut(s) 531
BssT1I CCWWGG 2 cut(s) 683, 1302
Bst2BI CACGAG 1 cut(s) 531
Bst4CI ACNGT 6 cut(s) 369, 432, 616, 893, 1003, 1284
Bst6I CTCTTC 1 cut(s) 63
BstAUI TGTACA 1 cut(s) 1095
BstC8I GCNNGC 4 cut(s) 207, 626, 834, 864
BstDEI CTNAG 4 cut(s) 44, 81, 164, 174
BstF5I GGATG 4 cut(s) 215, 460, 496, 757
BstFNI CGCG 1 cut(s) 1261
BstH2I RGCGCY 2 cut(s) 61, 644
BstHHI GCGC 3 cut(s) 60, 643, 832
BstKTI GATC 5 cut(s) 66, 75, 400, 596, 1222
BstMAI GTCTC 3 cut(s) 82, 605, 1156
BstMBI GATC 5 cut(s) 63, 72, 397, 593, 1219
BstMCI CGRYCG 1 cut(s) 66
BstMWI GCNNNNNNNGC 3 cut(s) 319, 336, 625
BstNSI RCATGY 4 cut(s) 270, 413, 1096, 1147
BstPAI GACNNNNGTC 1 cut(s) 1023
BstSCI CCNGG 1 cut(s) 1079
BstSFI CTRYAG 3 cut(s) 308, 375, 1059
BstSLI GKGCMC 1 cut(s) 301
BstUI CGCG 1 cut(s) 1261
BstV1I GCAGC 8 cut(s) 67, 250, 322, 361, 448, 451, 797, 1286
BstX2I RGATCY 2 cut(s) 593, 1219
BstYI RGATCY 2 cut(s) 593, 1219
BsuI GTATCC 1 cut(s) 986
BsuRI GGCC 3 cut(s) 108, 209, 673
BtsCI GGATG 4 cut(s) 215, 460, 496, 757
BtsIMutI CAGTG 4 cut(s) 661, 889, 1008, 1280
Cac8I GCNNGC 4 cut(s) 207, 626, 834, 864
CaiI CAGNNNCTG 1 cut(s) 521
CciI TCATGA 1 cut(s) 736
CfoI GCGC 3 cut(s) 60, 643, 832
Cfr13I GGNCC 2 cut(s) 656, 821
Csp6I GTAC 5 cut(s) 382, 407, 445, 796, 1096
CspCI CAANNNNNGTGG 2 cut(s) 1023, 1058
CviAII CATG 8 cut(s) 104, 267, 410, 425, 737, 1021, 1093, 1144
CviQI GTAC 5 cut(s) 382, 407, 445, 796, 1096
DdeI CTNAG 4 cut(s) 44, 81, 164, 174
DpnI GATC 5 cut(s) 65, 74, 399, 595, 1221
DpnII GATC 5 cut(s) 63, 72, 397, 593, 1219
DraI TTTAAA 1 cut(s) 816
EaeI YGGCCR 1 cut(s) 106
Eam1104I CTCTTC 1 cut(s) 63
EarI CTCTTC 1 cut(s) 63
Ecl136II GAGCTC 1 cut(s) 168
Eco130I CCWWGG 2 cut(s) 683, 1302
Eco147I AGGCCT 2 cut(s) 209, 673
Eco24I GRGCYC 1 cut(s) 170
Eco47I GGWCC 2 cut(s) 656, 821
Eco47III AGCGCT 1 cut(s) 642
Eco53kI GAGCTC 1 cut(s) 168
Eco57I CTGAAG 3 cut(s) 185, 387, 1166
EcoICRI GAGCTC 1 cut(s) 168
EcoO109I RGGNCCY 1 cut(s) 656
EcoT14I CCWWGG 2 cut(s) 683, 1302
EcoT22I ATGCAT 1 cut(s) 415
EcoT38I GRGCYC 1 cut(s) 170
ErhI CCWWGG 2 cut(s) 683, 1302
Esp3I CGTCTC 2 cut(s) 82, 605
FaeI CATG 8 cut(s) 107, 270, 413, 428, 740, 1024, 1096, 1147
FalI AAGNNNNNCTT 2 cut(s) 345, 377
FaqI GGGAC 1 cut(s) 834
FatI CATG 8 cut(s) 103, 266, 409, 424, 736, 1020, 1092, 1143
FokI GGATG 4 cut(s) 202, 447, 503, 764
FriOI GRGCYC 1 cut(s) 170
FspBI CTAG 4 cut(s) 342, 684, 710, 774
GlaI GCGC 3 cut(s) 59, 642, 831
GsaI CCCAGC 1 cut(s) 11
GsuI CTGGAG 2 cut(s) 594, 618
HaeII RGCGCY 2 cut(s) 61, 644
HaeIII GGCC 3 cut(s) 108, 209, 673
HapII CCGG 2 cut(s) 917, 1080
HhaI GCGC 3 cut(s) 60, 643, 832
Hin1II CATG 8 cut(s) 107, 270, 413, 428, 740, 1024, 1096, 1147
Hin6I GCGC 3 cut(s) 58, 641, 830
HinP1I GCGC 3 cut(s) 58, 641, 830
HincII GTYRAC 1 cut(s) 547
HindII GTYRAC 1 cut(s) 547
HindIII AAGCTT 2 cut(s) 810, 1239
HinfI GANTC 3 cut(s) 20, 358, 1024
HpaI GTTAAC 1 cut(s) 547
HpaII CCGG 2 cut(s) 917, 1080
HphI GGTGA 2 cut(s) 952, 1057
Hpy166II GTNNAC 3 cut(s) 547, 730, 1048
Hpy188I TCNGA 6 cut(s) 82, 334, 503, 699, 1185, 1196
Hpy188III TCNNGA 3 cut(s) 464, 597, 737
Hpy8I GTNNAC 3 cut(s) 547, 730, 1048
HpyAV CCTTC 6 cut(s) 19, 199, 237, 411, 641, 1086
HpyCH4III ACNGT 6 cut(s) 369, 432, 616, 893, 1003, 1284
HpyF10VI GCNNNNNNNGC 3 cut(s) 319, 336, 625
HpyF3I CTNAG 4 cut(s) 44, 81, 164, 174
Hsp92II CATG 8 cut(s) 107, 270, 413, 428, 740, 1024, 1096, 1147
HspAI GCGC 3 cut(s) 58, 641, 830
KspAI GTTAAC 1 cut(s) 547
Kzo9I GATC 5 cut(s) 63, 72, 397, 593, 1219
LmnI GCTCC 5 cut(s) 173, 575, 693, 859, 1296
Lsp1109I GCAGC 8 cut(s) 67, 250, 322, 361, 448, 451, 797, 1286
LweI GCATC 2 cut(s) 150, 481
MaeI CTAG 4 cut(s) 342, 684, 710, 774
MalI GATC 5 cut(s) 65, 74, 399, 595, 1221
MboI GATC 5 cut(s) 63, 72, 397, 593, 1219
MboII GAAGA 5 cut(s) 29, 80, 169, 191, 1172
MflI RGATCY 2 cut(s) 593, 1219
MhlI GDGCHC 2 cut(s) 170, 301
MluCI AATT 7 cut(s) 113, 247, 588, 634, 920, 984, 1105
MlyI GAGTC 1 cut(s) 1033
MmeI TCCRAC 1 cut(s) 837
MnlI CCTC 5 cut(s) 288, 540, 669, 773, 968
Mph1103I ATGCAT 1 cut(s) 415
MseI TTAA 6 cut(s) 246, 363, 546, 720, 815, 1215
MslI CAYNNNNRTG 1 cut(s) 1091
MspA1I CMGCKG 3 cut(s) 322, 788, 1277
MspI CCGG 2 cut(s) 917, 1080
MspR9I CCNGG 1 cut(s) 1081
MvnI CGCG 1 cut(s) 1261
MwoI GCNNNNNNNGC 3 cut(s) 319, 336, 625
NciI CCSGG 1 cut(s) 1081
NdeII GATC 5 cut(s) 63, 72, 397, 593, 1219
NlaIII CATG 8 cut(s) 107, 270, 413, 428, 740, 1024, 1096, 1147
NlaIV GGNNCC 3 cut(s) 689, 822, 1292
NsiI ATGCAT 1 cut(s) 415
NspI RCATGY 4 cut(s) 270, 413, 1096, 1147
PagI TCATGA 1 cut(s) 736
PceI AGGCCT 2 cut(s) 209, 673
PciI ACATGT 1 cut(s) 1092
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PfeI GAWTC 2 cut(s) 20, 358
Ple19I CGATCG 1 cut(s) 66
PleI GAGTC 1 cut(s) 1032
PpsI GAGTC 1 cut(s) 1032
PpuMI RGGWCCY 1 cut(s) 656
PscI ACATGT 1 cut(s) 1092
PshAI GACNNNNGTC 1 cut(s) 1023
PshBI ATTAAT 1 cut(s) 246
PsiI TTATAA 1 cut(s) 257
Psp124BI GAGCTC 1 cut(s) 170
Psp5II RGGWCCY 1 cut(s) 656
PspFI CCCAGC 1 cut(s) 7
PspN4I GGNNCC 3 cut(s) 689, 822, 1292
PspPI GGNCC 2 cut(s) 656, 821
PspPPI RGGWCCY 1 cut(s) 656
PstI CTGCAG 2 cut(s) 312, 379
PstNI CAGNNNCTG 1 cut(s) 521
PsuI RGATCY 2 cut(s) 593, 1219
PvuI CGATCG 1 cut(s) 66
PvuII CAGCTG 2 cut(s) 322, 788
RsaI GTAC 5 cut(s) 383, 408, 446, 797, 1097
RsaNI GTAC 5 cut(s) 382, 407, 445, 796, 1096
RseI CAYNNNNRTG 1 cut(s) 1091
SacI GAGCTC 1 cut(s) 170
SaqAI TTAA 6 cut(s) 246, 363, 546, 720, 815, 1215
Sau3AI GATC 5 cut(s) 63, 72, 397, 593, 1219
Sau96I GGNCC 2 cut(s) 656, 821
SchI GAGTC 1 cut(s) 1033
ScrFI CCNGG 1 cut(s) 1081
SduI GDGCHC 2 cut(s) 170, 301
SfaNI GCATC 2 cut(s) 150, 481
SfcI CTRYAG 3 cut(s) 308, 375, 1059
SinI GGWCC 2 cut(s) 656, 821
SmiMI CAYNNNNRTG 1 cut(s) 1091
SpeI ACTAGT 1 cut(s) 709
Sse9I AATT 7 cut(s) 113, 247, 588, 634, 920, 984, 1105
SseBI AGGCCT 2 cut(s) 209, 673
SsiI CCGC 4 cut(s) 109, 419, 1261, 1277
SspMI CTAG 4 cut(s) 342, 684, 710, 774
SstI GAGCTC 1 cut(s) 170
StuI AGGCCT 2 cut(s) 209, 673
StyD4I CCNGG 1 cut(s) 1079
StyI CCWWGG 2 cut(s) 683, 1302
TaaI ACNGT 6 cut(s) 369, 432, 616, 893, 1003, 1284
TaqI TCGA 4 cut(s) 23, 66, 276, 1230
TasI AATT 7 cut(s) 113, 247, 588, 634, 920, 984, 1105
TatI WGTACW 3 cut(s) 381, 406, 1095
TauI GCSGC 2 cut(s) 111, 1280
TfiI GAWTC 2 cut(s) 20, 358
Tru1I TTAA 6 cut(s) 246, 363, 546, 720, 815, 1215
Tru9I TTAA 6 cut(s) 246, 363, 546, 720, 815, 1215
TscAI CASTG 4 cut(s) 668, 896, 1008, 1287
TseI GCWGC 8 cut(s) 55, 263, 310, 374, 436, 439, 785, 1274
TspDTI ATGAA 8 cut(s) 17, 204, 232, 441, 720, 753, 1075, 1216
TspGWI ACGGA 2 cut(s) 228, 487
TspRI CASTG 4 cut(s) 668, 896, 1008, 1287
VpaK11BI GGWCC 2 cut(s) 656, 821
VspI ATTAAT 1 cut(s) 246
XceI RCATGY 4 cut(s) 270, 413, 1096, 1147
XcmI CCANNNNNNNNNTGG 1 cut(s) 640
XmaJI CCTAGG 1 cut(s) 683
XspI CTAG 4 cut(s) 342, 684, 710, 774
Zsp2I ATGCAT 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.