MD04G1064700.v1.1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005627
Physical Location & Seq
Forward (+)
8697125 .. 8698967
1843 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1064700.v1.1.491

Sequence Viewer

Length: 1023 bp
ATGTCCTCGTTGCCGGCCGAGCAGAACGGCGACTCGGCGCCTGCTGGAAGCGGTGGCGGCGGAGGAGATTCGCAGAGATCGCTTCCGACGCCGTTTTTGACCAAAACGTATCAGCTGGTTGACGATCCATCCGTCGATGATTTAATATCGTGGAGCGAGGATGGATCGGCGTTCATCGTCTGGCGACCTGCCGAATTCGCCAGAGATTTGCTTCCCAAGTACTTCAAACACAACAATTTCTCCAGCTTCGTTCGCCAGCTCAACACTTACGGATTCCGGAAAGTCGTGCCGGATCGATGGGAGTTCGCGAACGATTTCTTCAAGAAAGGCGAGAAAGGACTTCTCCGGGAGATTCAGCGCCGGAAAATCTCTCCATCCGTGTCGGCGGCAGCTACGGTTGTTCCGCCAGCGATGGCGGCGATATCCGCGTTGGCTCCCCGGGTGTCTCCGTCTAACTCCGGCGACGAGCAAGTGATCTCGTCCAATTCGTCTCCGGTGGCGGCTCCGGCAATGTTGGCTTGCCGAATCAGGAGCTCCACCTCCACGTCGGACGTTCTTGAAGAAAACGAGAGGTTGAGGAAAGAGAACATGCAGCTGAGTCACGAGTTGACTCAGCTCCGCGGGTTATGTAATAACATATTGGCGTTGATGACGAACTACGCGTCTGGTCAGTTAGACAGCGGTGGCGGTGGCGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGTGGATGACGGTAGGACTCTAGAGCTGTCGCCGGTGAAAGAGGCTGAGCCGGCTGCAGAAGACGCCGGTTGCAACGGAGCAAACGCGGAGGCTTCCGGGGCGGAAGAGGAGGAGGAGGAGGAAGAAGAGGAGGAGATGAGGCCGAGGTTGTTCGGGGTTTCGATCGGGGTGAAGCGCGTGAGGAGAGACGAAGACGAAGAGGAACAACATCGGGAAGGCTCCGAGGGGGCGAAGCAAGAGCCGTCGGATGGGAGCTTGAAGCGCGATTGTGACGCCACGTGGCTGGAGCTCGGAAAGTGA

Protein Analysis

341

Amino Acids

36.43

Weight (kDa)

4.69

Isoelectric Point (pI)

68.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 32 - 121 1.1e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 196
AccB1I GGYRCC 1 cut(s) 37
AccII CGCG 7 cut(s) 308, 428, 621, 662, 809, 900, 987
AccIII TCCGGA 1 cut(s) 276
AclWI GGATC 3 cut(s) 119, 172, 300
AcoI YGGCCR 1 cut(s) 15
AcsI RAATTY 1 cut(s) 194
AcvI CACGTG 1 cut(s) 1002
AcyI GRCGYC 4 cut(s) 38, 89, 786, 996
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 1 cut(s) 971
AflIII ACRYGT 1 cut(s) 660
AgsI TTSAA 4 cut(s) 226, 322, 560, 982
AjiI CACGTC 1 cut(s) 546
Alw21I GWGCWC 2 cut(s) 536, 1014
Alw26I GTCTC 3 cut(s) 450, 495, 903
AlwI GGATC 3 cut(s) 119, 172, 300
Ama87I CYCGRG 1 cut(s) 438
Aor13HI TCCGGA 1 cut(s) 276
AoxI GGCC 2 cut(s) 15, 863
ApeKI GCWGC 3 cut(s) 389, 592, 776
ApoI RAATTY 1 cut(s) 194
ArsI GACNNNNNNTTYG 4 cut(s) 79, 111, 647, 679
Asp700I GAANNNNTTC 1 cut(s) 314
AspLEI GCGC 4 cut(s) 40, 360, 900, 987
AsuC2I CCSGG 4 cut(s) 347, 439, 440, 820
AsuHPI GGTGA 2 cut(s) 769, 904
AvaI CYCGRG 1 cut(s) 438
BanI GGYRCC 1 cut(s) 37
BanII GRGCYC 2 cut(s) 536, 1014
BauI CACGAG 1 cut(s) 602
BbrPI CACGTG 1 cut(s) 1002
BbsI GAAGAC 2 cut(s) 789, 921
Bbv12I GWGCWC 2 cut(s) 536, 1014
BbvI GCAGC 3 cut(s) 401, 604, 763
BccI CCATC 6 cut(s) 136, 155, 291, 382, 406, 965
BceAI ACGGC 3 cut(s) 43, 76, 949
BcnI CCSGG 4 cut(s) 347, 439, 440, 820
BcoDI GTCTC 3 cut(s) 450, 495, 903
BfaI CTAG 1 cut(s) 743
BfmI CTRYAG 1 cut(s) 777
BfoI RGCGCY 2 cut(s) 41, 361
BfuAI ACCTGC 1 cut(s) 196
BisI GCNGC 7 cut(s) 58, 387, 390, 417, 501, 593, 777
BlpI GCTNAGC 1 cut(s) 768
BlsI GCNGC 7 cut(s) 59, 388, 391, 418, 502, 594, 778
BmcAI AGTACT 1 cut(s) 221
Bme1390I CCNGG 4 cut(s) 347, 439, 440, 820
BmeT110I CYCGRG 1 cut(s) 438
BmgBI CACGTC 1 cut(s) 546
BmiI GGNNCC 4 cut(s) 39, 435, 504, 943
BmrFI CCNGG 4 cut(s) 347, 439, 440, 820
BpiI GAAGAC 2 cut(s) 789, 921
BpmI CTGGAG 1 cut(s) 226
Bpu1102I GCTNAGC 1 cut(s) 768
BpuMI CCSGG 4 cut(s) 347, 439, 440, 820
Bsa29I ATCGAT 1 cut(s) 295
BsaAI YACGTR 1 cut(s) 1002
BsaHI GRCGYC 4 cut(s) 38, 89, 786, 996
BsaJI CCNNGG 6 cut(s) 437, 438, 619, 819, 866, 945
BsaWI WCCGGW 2 cut(s) 276, 493
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 708, 738
Bsc4I CCNNNNNNNGG 1 cut(s) 971
Bse118I RCCGGY 4 cut(s) 13, 754, 772, 788
Bse3DI GCAATG 1 cut(s) 516
BseAI TCCGGA 1 cut(s) 276
BseCI ATCGAT 1 cut(s) 295
BseDI CCNNGG 6 cut(s) 437, 438, 619, 819, 866, 945
BseGI GGATG 5 cut(s) 128, 166, 374, 733, 976
BseLI CCNNNNNNNGG 1 cut(s) 971
BseMI GCAATG 1 cut(s) 516
BseMII CTCAG 3 cut(s) 587, 626, 759
BseX3I CGGCCG 1 cut(s) 15
BseXI GCAGC 3 cut(s) 401, 604, 763
Bsh1236I CGCG 7 cut(s) 308, 428, 621, 662, 809, 900, 987
Bsh1285I CGRYCG 2 cut(s) 18, 888
BshFI GGCC 2 cut(s) 17, 865
BshNI GGYRCC 1 cut(s) 37
BshVI ATCGAT 1 cut(s) 295
BsiEI CGRYCG 2 cut(s) 18, 888
BsiHKAI GWGCWC 2 cut(s) 536, 1014
BsiHKCI CYCGRG 1 cut(s) 438
BslI CCNNNNNNNGG 1 cut(s) 971
BsmAI GTCTC 3 cut(s) 450, 495, 903
BsmBI CGTCTC 2 cut(s) 495, 903
BsnI GGCC 2 cut(s) 17, 865
BsoBI CYCGRG 1 cut(s) 438
Bsp1286I GDGCHC 2 cut(s) 536, 1014
Bsp13I TCCGGA 1 cut(s) 276
Bsp143I GATC 6 cut(s) 77, 124, 164, 292, 474, 885
Bsp1720I GCTNAGC 1 cut(s) 768
Bsp68I TCGCGA 1 cut(s) 308
BspANI GGCC 2 cut(s) 17, 865
BspCNI CTCAG 3 cut(s) 588, 625, 760
BspDI ATCGAT 1 cut(s) 295
BspEI TCCGGA 1 cut(s) 276
BspFNI CGCG 7 cut(s) 308, 428, 621, 662, 809, 900, 987
BspLI GGNNCC 4 cut(s) 39, 435, 504, 943
BspMAI CTGCAG 1 cut(s) 781
BspMI ACCTGC 1 cut(s) 196
BspPI GGATC 3 cut(s) 119, 172, 300
BspT107I GGYRCC 1 cut(s) 37
BsrDI GCAATG 1 cut(s) 516
BsrFI RCCGGY 4 cut(s) 13, 754, 772, 788
BssAI RCCGGY 4 cut(s) 13, 754, 772, 788
BssECI CCNNGG 6 cut(s) 437, 438, 619, 819, 866, 945
BssMI GATC 6 cut(s) 77, 124, 164, 292, 474, 885
BssNI GRCGYC 4 cut(s) 38, 89, 786, 996
BssSI CACGAG 1 cut(s) 602
Bst2BI CACGAG 1 cut(s) 602
Bst4CI ACNGT 2 cut(s) 397, 734
Bst6I CTCTTC 3 cut(s) 822, 843, 915
BstACI GRCGYC 4 cut(s) 38, 89, 786, 996
BstBAI YACGTR 1 cut(s) 1002
BstC8I GCNNGC 6 cut(s) 15, 42, 257, 408, 520, 774
BstDEI CTNAG 3 cut(s) 596, 612, 768
BstDSI CCRYGG 1 cut(s) 619
BstF5I GGATG 5 cut(s) 128, 166, 374, 733, 976
BstFNI CGCG 7 cut(s) 308, 428, 621, 662, 809, 900, 987
BstH2I RGCGCY 2 cut(s) 41, 361
BstHHI GCGC 4 cut(s) 40, 360, 900, 987
BstKTI GATC 6 cut(s) 80, 127, 167, 295, 477, 888
BstMAI GTCTC 3 cut(s) 450, 495, 903
BstMBI GATC 6 cut(s) 77, 124, 164, 292, 474, 885
BstMCI CGRYCG 2 cut(s) 18, 888
BstNSI RCATGY 1 cut(s) 592
BstSCI CCNGG 4 cut(s) 345, 437, 438, 818
BstSFI CTRYAG 1 cut(s) 777
BstUI CGCG 7 cut(s) 308, 428, 621, 662, 809, 900, 987
BstV1I GCAGC 3 cut(s) 401, 604, 763
BstV2I GAAGAC 2 cut(s) 789, 921
BstXI CCANNNNNNTGG 1 cut(s) 1006
BstZI CGGCCG 1 cut(s) 15
Bsu15I ATCGAT 1 cut(s) 295
BsuRI GGCC 2 cut(s) 17, 865
BsuTUI ATCGAT 1 cut(s) 295
BtgI CCRYGG 1 cut(s) 619
BtgZI GCGATG 1 cut(s) 425
BtrI CACGTC 1 cut(s) 546
BtsCI GGATG 5 cut(s) 128, 166, 374, 733, 976
BtuMI TCGCGA 1 cut(s) 308
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 6 cut(s) 15, 42, 257, 408, 520, 774
CfoI GCGC 4 cut(s) 40, 360, 900, 987
Cfr10I RCCGGY 4 cut(s) 13, 754, 772, 788
Cfr42I CCGCGG 1 cut(s) 622
Cfr9I CCCGGG 1 cut(s) 438
ClaI ATCGAT 1 cut(s) 295
CseI GACGC 4 cut(s) 97, 651, 794, 1004
Csp6I GTAC 1 cut(s) 220
CviAII CATG 1 cut(s) 589
CviQI GTAC 1 cut(s) 220
DdeI CTNAG 3 cut(s) 596, 612, 768
DinI GGCGCC 1 cut(s) 39
DpnI GATC 6 cut(s) 79, 126, 166, 294, 476, 887
DpnII GATC 6 cut(s) 77, 124, 164, 292, 474, 885
EaeI YGGCCR 1 cut(s) 15
EagI CGGCCG 1 cut(s) 15
Eam1104I CTCTTC 3 cut(s) 822, 843, 915
EarI CTCTTC 3 cut(s) 822, 843, 915
EciI GGCGGA 4 cut(s) 75, 393, 708, 839
Ecl136II GAGCTC 2 cut(s) 534, 1012
EclXI CGGCCG 1 cut(s) 15
Eco24I GRGCYC 2 cut(s) 536, 1014
Eco32I GATATC 1 cut(s) 423
Eco52I CGGCCG 1 cut(s) 15
Eco53kI GAGCTC 2 cut(s) 534, 1012
Eco72I CACGTG 1 cut(s) 1002
Eco88I CYCGRG 1 cut(s) 438
EcoICRI GAGCTC 2 cut(s) 534, 1012
EcoRI GAATTC 1 cut(s) 194
EcoRV GATATC 1 cut(s) 423
EcoT38I GRGCYC 2 cut(s) 536, 1014
EgeI GGCGCC 1 cut(s) 39
EheI GGCGCC 1 cut(s) 39
Esp3I CGTCTC 2 cut(s) 495, 903
FaeI CATG 1 cut(s) 592
FaiI YATR 3 cut(s) 590, 628, 638
FatI CATG 1 cut(s) 588
FauI CCCGC 1 cut(s) 614
Fnu4HI GCNGC 7 cut(s) 58, 387, 390, 417, 501, 593, 777
FokI GGATG 5 cut(s) 115, 173, 361, 740, 983
FriOI GRGCYC 2 cut(s) 536, 1014
Fsp4HI GCNGC 7 cut(s) 58, 387, 390, 417, 501, 593, 777
FspBI CTAG 1 cut(s) 743
GlaI GCGC 4 cut(s) 39, 359, 899, 986
GluI GCNGC 7 cut(s) 58, 387, 390, 417, 501, 593, 777
GsuI CTGGAG 1 cut(s) 226
HaeII RGCGCY 2 cut(s) 41, 361
HaeIII GGCC 2 cut(s) 17, 865
HgaI GACGC 4 cut(s) 97, 651, 794, 1004
HhaI GCGC 4 cut(s) 40, 360, 900, 987
Hin1I GRCGYC 4 cut(s) 38, 89, 786, 996
Hin1II CATG 1 cut(s) 592
Hin6I GCGC 4 cut(s) 38, 358, 898, 985
HinP1I GCGC 4 cut(s) 38, 358, 898, 985
HincII GTYRAC 2 cut(s) 121, 609
HindII GTYRAC 2 cut(s) 121, 609
HinfI GANTC 8 cut(s) 32, 68, 273, 352, 525, 598, 610, 739
HphI GGTGA 2 cut(s) 769, 904
Hpy166II GTNNAC 2 cut(s) 121, 609
Hpy188I TCNGA 5 cut(s) 87, 550, 946, 970, 1016
Hpy188III TCNNGA 8 cut(s) 277, 307, 322, 529, 557, 602, 743, 935
Hpy8I GTNNAC 2 cut(s) 121, 609
Hpy99I CGWCG 5 cut(s) 91, 137, 467, 550, 970
HpyAV CCTTC 1 cut(s) 932
HpyCH4III ACNGT 2 cut(s) 397, 734
HpyCH4IV ACGT 4 cut(s) 107, 545, 552, 1001
HpyCH4V TGCA 3 cut(s) 592, 779, 795
HpyF3I CTNAG 3 cut(s) 596, 612, 768
HpySE526I ACGT 4 cut(s) 107, 545, 552, 1001
Hsp92I GRCGYC 4 cut(s) 38, 89, 786, 996
Hsp92II CATG 1 cut(s) 592
HspAI GCGC 4 cut(s) 38, 358, 898, 985
KasI GGCGCC 1 cut(s) 37
Kpn2I TCCGGA 1 cut(s) 276
KroI GCCGGC 2 cut(s) 13, 772
KroNI GCCGGC 2 cut(s) 15, 774
KspI CCGCGG 1 cut(s) 622
Kzo9I GATC 6 cut(s) 77, 124, 164, 292, 474, 885
Lsp1109I GCAGC 3 cut(s) 401, 604, 763
MaeI CTAG 1 cut(s) 743
MaeII ACGT 4 cut(s) 107, 545, 552, 1001
MaeIII GTNAC 2 cut(s) 599, 992
MalI GATC 6 cut(s) 79, 126, 166, 294, 476, 887
MboI GATC 6 cut(s) 77, 124, 164, 292, 474, 885
MboII GAAGA 8 cut(s) 310, 572, 794, 839, 857, 860, 926, 932
MhlI GDGCHC 2 cut(s) 536, 1014
MluCI AATT 3 cut(s) 194, 235, 484
MluI ACGCGT 1 cut(s) 660
Mly113I GGCGCC 1 cut(s) 38
MlyI GAGTC 4 cut(s) 26, 604, 607, 733
MmeI TCCRAC 3 cut(s) 110, 528, 948
MroI TCCGGA 1 cut(s) 276
MroNI GCCGGC 2 cut(s) 13, 772
MroXI GAANNNNTTC 1 cut(s) 314
MseI TTAA 1 cut(s) 143
MspA1I CMGCKG 4 cut(s) 115, 595, 621, 681
MspR9I CCNGG 4 cut(s) 347, 439, 440, 820
MvnI CGCG 7 cut(s) 308, 428, 621, 662, 809, 900, 987
NaeI GCCGGC 2 cut(s) 15, 774
NarI GGCGCC 1 cut(s) 38
NciI CCSGG 4 cut(s) 347, 439, 440, 820
NdeII GATC 6 cut(s) 77, 124, 164, 292, 474, 885
NgoMIV GCCGGC 2 cut(s) 13, 772
NlaIII CATG 1 cut(s) 592
NlaIV GGNNCC 4 cut(s) 39, 435, 504, 943
NmeAIII GCCGAG 3 cut(s) 14, 43, 891
NmuCI GTSAC 2 cut(s) 599, 992
NruI TCGCGA 1 cut(s) 308
NspI RCATGY 1 cut(s) 592
PcsI WCGNNNNNNNCGW 3 cut(s) 129, 485, 659
PdiI GCCGGC 2 cut(s) 15, 774
PdmI GAANNNNTTC 1 cut(s) 314
PfeI GAWTC 4 cut(s) 68, 273, 352, 525
PfoI TCCNGGA 1 cut(s) 345
PkrI GCNGC 7 cut(s) 59, 388, 391, 418, 502, 594, 778
Ple19I CGATCG 1 cut(s) 888
PleI GAGTC 4 cut(s) 26, 604, 606, 733
PluTI GGCGCC 1 cut(s) 41
PmaCI CACGTG 1 cut(s) 1002
PmlI CACGTG 1 cut(s) 1002
PpsI GAGTC 4 cut(s) 26, 604, 606, 733
Ppu21I YACGTR 1 cut(s) 1002
Psp124BI GAGCTC 2 cut(s) 536, 1014
PspCI CACGTG 1 cut(s) 1002
PspN4I GGNNCC 4 cut(s) 39, 435, 504, 943
PstI CTGCAG 1 cut(s) 781
PvuI CGATCG 1 cut(s) 888
PvuII CAGCTG 2 cut(s) 115, 595
RruI TCGCGA 1 cut(s) 308
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
SacI GAGCTC 2 cut(s) 536, 1014
SacII CCGCGG 1 cut(s) 622
SaqAI TTAA 1 cut(s) 143
SatI GCNGC 7 cut(s) 58, 387, 390, 417, 501, 593, 777
Sau3AI GATC 6 cut(s) 77, 124, 164, 292, 474, 885
ScaI AGTACT 1 cut(s) 221
SchI GAGTC 4 cut(s) 26, 604, 607, 733
ScrFI CCNGG 4 cut(s) 347, 439, 440, 820
SduI GDGCHC 2 cut(s) 536, 1014
SfcI CTRYAG 1 cut(s) 777
SfoI GGCGCC 1 cut(s) 39
Sfr303I CCGCGG 1 cut(s) 622
SgrAI CRCCGGYG 1 cut(s) 754
SgrBI CCGCGG 1 cut(s) 622
SmaI CCCGGG 1 cut(s) 440
Sse9I AATT 3 cut(s) 194, 235, 484
SspDI GGCGCC 1 cut(s) 37
SspMI CTAG 1 cut(s) 743
SstI GAGCTC 2 cut(s) 536, 1014
StyD4I CCNGG 4 cut(s) 345, 437, 438, 818
TaaI ACNGT 2 cut(s) 397, 734
TaiI ACGT 4 cut(s) 110, 548, 555, 1004
TaqI TCGA 3 cut(s) 135, 295, 884
TasI AATT 3 cut(s) 194, 235, 484
TatI WGTACW 1 cut(s) 219
TauI GCSGC 4 cut(s) 60, 389, 419, 503
TfiI GAWTC 4 cut(s) 68, 273, 352, 525
Tru1I TTAA 1 cut(s) 143
Tru9I TTAA 1 cut(s) 143
TseFI GTSAC 2 cut(s) 599, 992
TseI GCWGC 3 cut(s) 389, 592, 776
Tsp45I GTSAC 2 cut(s) 599, 992
TspDTI ATGAA 1 cut(s) 163
TspGWI ACGGA 5 cut(s) 121, 285, 367, 438, 813
TspMI CCCGGG 1 cut(s) 438
XapI RAATTY 1 cut(s) 194
XbaI TCTAGA 1 cut(s) 742
XceI RCATGY 1 cut(s) 592
XmaI CCCGGG 1 cut(s) 438
XmnI GAANNNNTTC 1 cut(s) 314
XspI CTAG 1 cut(s) 743
ZrmI AGTACT 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.