pycom04g05850

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005627
Physical Location & Seq
Forward (+)
5696612 .. 5697742
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g05850.1

Sequence Viewer

Length: 1005 bp
ATGTCCTCGTTGCCGGCCGAGCAGAACGGCGACTCGGCGCCTGCTGGAAGCGGTGGCGGCGGAGGAGATTCGCAGAGATCGCTTCCGACGCCGTTTTTGACCAAAACATATCAGCTGGTTGACGATCCATCCGTCGATGATTTAATATCGTGGAGCGAGGATGGATCGGCGTTCATCGTCTGGCGACCTGCCGAATTCGCCAGAGATTTGCTTCCCAAGTACTTCAAACACAACAATTTCTCCAGTTTCGTTCGCCAGCTCAACACTTACGGATTCCGGAAAGTCGTGCCGGATCGATGGGAGTTCGCAAACGATTTCTTCAAGAAAGGCGAGAAAGGACTTCTCCGGGAGATTCAGCGCCGGAAAATCTCTCCATCGGTGTCGGCGGCAGCTACGGTTGTTCCGCCAGCGATGGCGGCGATATCGGCGTTGGCTCCCCGGGTGTCTCCGTCTAACTCCGGCGACGAGCAAGTGATCTCGTCGAATTCGTCTCCGGTGGCGGCTCCGGCAATGTTGGCGAGCCGAATCAGGAGCTCCACCTCCACGTCGGACGTTCTAGAAGAAAACGAGCGGCTGAGGAAAGAGAACATGCATCTGAGTCACGAGTTGACTCAGCTCCGCGGGTTATGTAATAACATATTGGCGTTGATGACGAACTACGCGTCTGGTCAGTTAGAGAGCGGTGGCGGAGGAGGAGGAGGAGGAGTAGTGGATGACGGTAGGACTTTAGAGCTGTCGCCGGTGAAAGAGGCTGAGCCTGCTGCAGAAGACGCCGGTTGCAACGGAGCGAACGCGGAGGCTTCCGGGGCGGAAGAGGAGGAGGAGGAAGAAGAGGAGGAGATGAGGCCAAGGTTGTTCGGGGTTTCGATCGGGGTGAAGCGCGTGAGGAGAGACGAAGACGAAGAGGAACAGCATCGGGAAGGCTCCGAGGGGGCGAAGCAAGAGCCGTCGGATGGGAGCTTGAAGCGCGATTGTGACGCCACGTGGCTGGAGCTCGGAAAGTGA

Protein Analysis

335

Amino Acids

36.06

Weight (kDa)

4.76

Isoelectric Point (pI)

67.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 32 - 121 1.1e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 196
AccB1I GGYRCC 1 cut(s) 37
AccBSI CCGCTC 2 cut(s) 571, 681
AccII CGCG 5 cut(s) 621, 662, 794, 882, 969
AccIII TCCGGA 1 cut(s) 276
AclWI GGATC 3 cut(s) 119, 172, 300
AcoI YGGCCR 1 cut(s) 15
AcsI RAATTY 2 cut(s) 194, 484
AcvI CACGTG 1 cut(s) 984
AcyI GRCGYC 4 cut(s) 38, 89, 771, 978
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 1 cut(s) 953
AflIII ACRYGT 1 cut(s) 660
AgsI TTSAA 3 cut(s) 226, 322, 964
AjiI CACGTC 1 cut(s) 546
AluBI AGCT 8 cut(s) 115, 259, 392, 534, 616, 733, 960, 994
AluI AGCT 8 cut(s) 115, 259, 392, 534, 616, 733, 960, 994
Alw21I GWGCWC 2 cut(s) 536, 996
Alw26I GTCTC 3 cut(s) 450, 495, 885
AlwI GGATC 3 cut(s) 119, 172, 300
Ama87I CYCGRG 1 cut(s) 438
Aor13HI TCCGGA 1 cut(s) 276
AoxI GGCC 2 cut(s) 15, 845
ApeKI GCWGC 2 cut(s) 389, 761
ApoI RAATTY 2 cut(s) 194, 484
ArsI GACNNNNNNTTYG 4 cut(s) 79, 111, 647, 679
AspLEI GCGC 4 cut(s) 40, 360, 882, 969
AsuC2I CCSGG 4 cut(s) 347, 439, 440, 805
AsuHPI GGTGA 2 cut(s) 754, 886
AvaI CYCGRG 1 cut(s) 438
BanI GGYRCC 1 cut(s) 37
BanII GRGCYC 2 cut(s) 536, 996
BauI CACGAG 1 cut(s) 602
BbrPI CACGTG 1 cut(s) 984
BbsI GAAGAC 2 cut(s) 774, 903
Bbv12I GWGCWC 2 cut(s) 536, 996
BbvCI CCTCAGC 1 cut(s) 575
BbvI GCAGC 2 cut(s) 401, 748
BccI CCATC 6 cut(s) 136, 155, 291, 382, 406, 947
BceAI ACGGC 3 cut(s) 43, 76, 931
BcnI CCSGG 4 cut(s) 347, 439, 440, 805
BcoDI GTCTC 3 cut(s) 450, 495, 885
BfaI CTAG 1 cut(s) 557
BfmI CTRYAG 1 cut(s) 762
BfoI RGCGCY 2 cut(s) 41, 361
BfuAI ACCTGC 1 cut(s) 196
BisI GCNGC 7 cut(s) 58, 387, 390, 417, 501, 572, 762
BlpI GCTNAGC 1 cut(s) 753
BlsI GCNGC 7 cut(s) 59, 388, 391, 418, 502, 573, 763
BmcAI AGTACT 1 cut(s) 221
Bme1390I CCNGG 4 cut(s) 347, 439, 440, 805
BmeT110I CYCGRG 1 cut(s) 438
BmgBI CACGTC 1 cut(s) 546
BmiI GGNNCC 4 cut(s) 39, 435, 504, 925
BmrFI CCNGG 4 cut(s) 347, 439, 440, 805
BmsI GCATC 2 cut(s) 601, 922
BpiI GAAGAC 2 cut(s) 774, 903
BpmI CTGGAG 1 cut(s) 226
Bpu10I CCTNAGC 1 cut(s) 575
Bpu1102I GCTNAGC 1 cut(s) 753
BpuMI CCSGG 4 cut(s) 347, 439, 440, 805
Bsa29I ATCGAT 1 cut(s) 295
BsaAI YACGTR 1 cut(s) 984
BsaHI GRCGYC 4 cut(s) 38, 89, 771, 978
BsaJI CCNNGG 6 cut(s) 437, 438, 619, 804, 848, 927
BsaWI WCCGGW 2 cut(s) 276, 493
BsaXI ACNNNNNCTCC 6 cut(s) 224, 254, 690, 693, 720, 723
Bsc4I CCNNNNNNNGG 1 cut(s) 953
Bse118I RCCGGY 3 cut(s) 13, 739, 773
Bse1I ACTGG 1 cut(s) 243
Bse3DI GCAATG 1 cut(s) 516
BseAI TCCGGA 1 cut(s) 276
BseCI ATCGAT 1 cut(s) 295
BseDI CCNNGG 6 cut(s) 437, 438, 619, 804, 848, 927
BseGI GGATG 4 cut(s) 128, 166, 718, 958
BseLI CCNNNNNNNGG 1 cut(s) 953
BseMI GCAATG 1 cut(s) 516
BseMII CTCAG 4 cut(s) 566, 587, 626, 744
BseNI ACTGG 1 cut(s) 243
BseX3I CGGCCG 1 cut(s) 15
BseXI GCAGC 2 cut(s) 401, 748
Bsh1236I CGCG 5 cut(s) 621, 662, 794, 882, 969
Bsh1285I CGRYCG 2 cut(s) 18, 870
BshFI GGCC 2 cut(s) 17, 847
BshNI GGYRCC 1 cut(s) 37
BshVI ATCGAT 1 cut(s) 295
BsiEI CGRYCG 2 cut(s) 18, 870
BsiHKAI GWGCWC 2 cut(s) 536, 996
BsiHKCI CYCGRG 1 cut(s) 438
BslI CCNNNNNNNGG 1 cut(s) 953
BsmAI GTCTC 3 cut(s) 450, 495, 885
BsmBI CGTCTC 2 cut(s) 495, 885
BsnI GGCC 2 cut(s) 17, 847
BsoBI CYCGRG 1 cut(s) 438
Bsp1286I GDGCHC 2 cut(s) 536, 996
Bsp13I TCCGGA 1 cut(s) 276
Bsp143I GATC 6 cut(s) 77, 124, 164, 292, 474, 867
Bsp1720I GCTNAGC 1 cut(s) 753
BspANI GGCC 2 cut(s) 17, 847
BspCNI CTCAG 4 cut(s) 567, 588, 625, 745
BspDI ATCGAT 1 cut(s) 295
BspEI TCCGGA 1 cut(s) 276
BspFNI CGCG 5 cut(s) 621, 662, 794, 882, 969
BspLI GGNNCC 4 cut(s) 39, 435, 504, 925
BspMAI CTGCAG 1 cut(s) 766
BspMI ACCTGC 1 cut(s) 196
BspPI GGATC 3 cut(s) 119, 172, 300
BspT107I GGYRCC 1 cut(s) 37
BsrBI CCGCTC 2 cut(s) 571, 681
BsrDI GCAATG 1 cut(s) 516
BsrFI RCCGGY 3 cut(s) 13, 739, 773
BsrI ACTGG 1 cut(s) 243
BssAI RCCGGY 3 cut(s) 13, 739, 773
BssECI CCNNGG 6 cut(s) 437, 438, 619, 804, 848, 927
BssMI GATC 6 cut(s) 77, 124, 164, 292, 474, 867
BssNI GRCGYC 4 cut(s) 38, 89, 771, 978
BssSI CACGAG 1 cut(s) 602
BssT1I CCWWGG 1 cut(s) 848
Bst2BI CACGAG 1 cut(s) 602
Bst4CI ACNGT 2 cut(s) 397, 719
Bst6I CTCTTC 3 cut(s) 807, 825, 897
BstACI GRCGYC 4 cut(s) 38, 89, 771, 978
BstBAI YACGTR 1 cut(s) 984
BstC8I GCNNGC 6 cut(s) 15, 42, 257, 408, 520, 759
BstDEI CTNAG 4 cut(s) 575, 596, 612, 753
BstDSI CCRYGG 1 cut(s) 619
BstF5I GGATG 4 cut(s) 128, 166, 718, 958
BstFNI CGCG 5 cut(s) 621, 662, 794, 882, 969
BstH2I RGCGCY 2 cut(s) 41, 361
BstHHI GCGC 4 cut(s) 40, 360, 882, 969
BstKTI GATC 6 cut(s) 80, 127, 167, 295, 477, 870
BstMAI GTCTC 3 cut(s) 450, 495, 885
BstMBI GATC 6 cut(s) 77, 124, 164, 292, 474, 867
BstMCI CGRYCG 2 cut(s) 18, 870
BstNSI RCATGY 1 cut(s) 592
BstSCI CCNGG 4 cut(s) 345, 437, 438, 803
BstSFI CTRYAG 1 cut(s) 762
BstUI CGCG 5 cut(s) 621, 662, 794, 882, 969
BstV1I GCAGC 2 cut(s) 401, 748
BstV2I GAAGAC 2 cut(s) 774, 903
BstXI CCANNNNNNTGG 1 cut(s) 988
BstZI CGGCCG 1 cut(s) 15
Bsu15I ATCGAT 1 cut(s) 295
BsuRI GGCC 2 cut(s) 17, 847
BsuTUI ATCGAT 1 cut(s) 295
BtgI CCRYGG 1 cut(s) 619
BtgZI GCGATG 1 cut(s) 425
BtrI CACGTC 1 cut(s) 546
BtsCI GGATG 4 cut(s) 128, 166, 718, 958
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 6 cut(s) 15, 42, 257, 408, 520, 759
CfoI GCGC 4 cut(s) 40, 360, 882, 969
Cfr10I RCCGGY 3 cut(s) 13, 739, 773
Cfr42I CCGCGG 1 cut(s) 622
Cfr9I CCCGGG 1 cut(s) 438
ClaI ATCGAT 1 cut(s) 295
CseI GACGC 4 cut(s) 97, 651, 779, 986
Csp6I GTAC 1 cut(s) 220
CviAII CATG 1 cut(s) 589
CviQI GTAC 1 cut(s) 220
DdeI CTNAG 4 cut(s) 575, 596, 612, 753
DinI GGCGCC 1 cut(s) 39
DpnI GATC 6 cut(s) 79, 126, 166, 294, 476, 869
DpnII GATC 6 cut(s) 77, 124, 164, 292, 474, 867
EaeI YGGCCR 1 cut(s) 15
EagI CGGCCG 1 cut(s) 15
Eam1104I CTCTTC 3 cut(s) 807, 825, 897
EarI CTCTTC 3 cut(s) 807, 825, 897
EciI GGCGGA 4 cut(s) 75, 393, 702, 824
Ecl136II GAGCTC 2 cut(s) 534, 994
EclXI CGGCCG 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 848
Eco24I GRGCYC 2 cut(s) 536, 996
Eco32I GATATC 1 cut(s) 423
Eco52I CGGCCG 1 cut(s) 15
Eco53kI GAGCTC 2 cut(s) 534, 994
Eco72I CACGTG 1 cut(s) 984
Eco88I CYCGRG 1 cut(s) 438
EcoICRI GAGCTC 2 cut(s) 534, 994
EcoRI GAATTC 2 cut(s) 194, 484
EcoRV GATATC 1 cut(s) 423
EcoT14I CCWWGG 1 cut(s) 848
EcoT22I ATGCAT 1 cut(s) 594
EcoT38I GRGCYC 2 cut(s) 536, 996
EgeI GGCGCC 1 cut(s) 39
EheI GGCGCC 1 cut(s) 39
ErhI CCWWGG 1 cut(s) 848
Esp3I CGTCTC 2 cut(s) 495, 885
FaeI CATG 1 cut(s) 592
FaiI YATR 4 cut(s) 109, 590, 628, 638
FatI CATG 1 cut(s) 588
FauI CCCGC 1 cut(s) 614
Fnu4HI GCNGC 7 cut(s) 58, 387, 390, 417, 501, 572, 762
FokI GGATG 4 cut(s) 115, 173, 725, 965
FriOI GRGCYC 2 cut(s) 536, 996
Fsp4HI GCNGC 7 cut(s) 58, 387, 390, 417, 501, 572, 762
FspBI CTAG 1 cut(s) 557
GlaI GCGC 4 cut(s) 39, 359, 881, 968
GluI GCNGC 7 cut(s) 58, 387, 390, 417, 501, 572, 762
GsuI CTGGAG 1 cut(s) 226
HaeII RGCGCY 2 cut(s) 41, 361
HaeIII GGCC 2 cut(s) 17, 847
HgaI GACGC 4 cut(s) 97, 651, 779, 986
HhaI GCGC 4 cut(s) 40, 360, 882, 969
Hin1I GRCGYC 4 cut(s) 38, 89, 771, 978
Hin1II CATG 1 cut(s) 592
Hin6I GCGC 4 cut(s) 38, 358, 880, 967
HinP1I GCGC 4 cut(s) 38, 358, 880, 967
HincII GTYRAC 2 cut(s) 121, 609
HindII GTYRAC 2 cut(s) 121, 609
HinfI GANTC 7 cut(s) 32, 68, 273, 352, 525, 598, 610
HphI GGTGA 2 cut(s) 754, 886
Hpy166II GTNNAC 2 cut(s) 121, 609
Hpy188I TCNGA 6 cut(s) 87, 550, 597, 928, 952, 998
Hpy188III TCNNGA 6 cut(s) 277, 322, 529, 557, 602, 917
Hpy8I GTNNAC 2 cut(s) 121, 609
Hpy99I CGWCG 6 cut(s) 91, 137, 467, 484, 550, 952
HpyAV CCTTC 1 cut(s) 914
HpyCH4III ACNGT 2 cut(s) 397, 719
HpyCH4IV ACGT 3 cut(s) 545, 552, 983
HpyCH4V TGCA 3 cut(s) 592, 764, 780
HpyF3I CTNAG 4 cut(s) 575, 596, 612, 753
HpySE526I ACGT 3 cut(s) 545, 552, 983
Hsp92I GRCGYC 4 cut(s) 38, 89, 771, 978
Hsp92II CATG 1 cut(s) 592
HspAI GCGC 4 cut(s) 38, 358, 880, 967
KasI GGCGCC 1 cut(s) 37
Kpn2I TCCGGA 1 cut(s) 276
KroI GCCGGC 1 cut(s) 13
KroNI GCCGGC 1 cut(s) 15
KspI CCGCGG 1 cut(s) 622
Kzo9I GATC 6 cut(s) 77, 124, 164, 292, 474, 867
Lsp1109I GCAGC 2 cut(s) 401, 748
LweI GCATC 2 cut(s) 601, 922
MaeI CTAG 1 cut(s) 557
MaeII ACGT 3 cut(s) 545, 552, 983
MaeIII GTNAC 2 cut(s) 599, 974
MalI GATC 6 cut(s) 79, 126, 166, 294, 476, 869
MbiI CCGCTC 2 cut(s) 571, 681
MboI GATC 6 cut(s) 77, 124, 164, 292, 474, 867
MboII GAAGA 8 cut(s) 310, 572, 779, 824, 839, 842, 908, 914
MhlI GDGCHC 2 cut(s) 536, 996
MluCI AATT 3 cut(s) 194, 235, 484
MluI ACGCGT 1 cut(s) 660
Mly113I GGCGCC 1 cut(s) 38
MlyI GAGTC 3 cut(s) 26, 604, 607
MmeI TCCRAC 3 cut(s) 110, 528, 930
Mph1103I ATGCAT 1 cut(s) 594
MroI TCCGGA 1 cut(s) 276
MroNI GCCGGC 1 cut(s) 13
MseI TTAA 1 cut(s) 143
MspA1I CMGCKG 2 cut(s) 115, 621
MspR9I CCNGG 4 cut(s) 347, 439, 440, 805
MvnI CGCG 5 cut(s) 621, 662, 794, 882, 969
NaeI GCCGGC 1 cut(s) 15
NarI GGCGCC 1 cut(s) 38
NciI CCSGG 4 cut(s) 347, 439, 440, 805
NdeII GATC 6 cut(s) 77, 124, 164, 292, 474, 867
NgoMIV GCCGGC 1 cut(s) 13
NlaIII CATG 1 cut(s) 592
NlaIV GGNNCC 4 cut(s) 39, 435, 504, 925
NmeAIII GCCGAG 2 cut(s) 14, 43
NmuCI GTSAC 2 cut(s) 599, 974
NsiI ATGCAT 1 cut(s) 594
NspI RCATGY 1 cut(s) 592
PcsI WCGNNNNNNNCGW 3 cut(s) 129, 485, 659
PdiI GCCGGC 1 cut(s) 15
PfeI GAWTC 4 cut(s) 68, 273, 352, 525
PfoI TCCNGGA 1 cut(s) 345
PkrI GCNGC 7 cut(s) 59, 388, 391, 418, 502, 573, 763
Ple19I CGATCG 1 cut(s) 870
PleI GAGTC 3 cut(s) 26, 604, 606
PluTI GGCGCC 1 cut(s) 41
PmaCI CACGTG 1 cut(s) 984
PmlI CACGTG 1 cut(s) 984
PpsI GAGTC 3 cut(s) 26, 604, 606
Ppu21I YACGTR 1 cut(s) 984
Psp124BI GAGCTC 2 cut(s) 536, 996
PspCI CACGTG 1 cut(s) 984
PspN4I GGNNCC 4 cut(s) 39, 435, 504, 925
PstI CTGCAG 1 cut(s) 766
PvuI CGATCG 1 cut(s) 870
PvuII CAGCTG 1 cut(s) 115
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
SacI GAGCTC 2 cut(s) 536, 996
SacII CCGCGG 1 cut(s) 622
SaqAI TTAA 1 cut(s) 143
SatI GCNGC 7 cut(s) 58, 387, 390, 417, 501, 572, 762
Sau3AI GATC 6 cut(s) 77, 124, 164, 292, 474, 867
ScaI AGTACT 1 cut(s) 221
SchI GAGTC 3 cut(s) 26, 604, 607
ScrFI CCNGG 4 cut(s) 347, 439, 440, 805
SduI GDGCHC 2 cut(s) 536, 996
SfaNI GCATC 2 cut(s) 601, 922
SfcI CTRYAG 1 cut(s) 762
SfoI GGCGCC 1 cut(s) 39
Sfr303I CCGCGG 1 cut(s) 622
SgrAI CRCCGGYG 1 cut(s) 739
SgrBI CCGCGG 1 cut(s) 622
SmaI CCCGGG 1 cut(s) 440
Sse9I AATT 3 cut(s) 194, 235, 484
SspDI GGCGCC 1 cut(s) 37
SspMI CTAG 1 cut(s) 557
SstI GAGCTC 2 cut(s) 536, 996
StyD4I CCNGG 4 cut(s) 345, 437, 438, 803
StyI CCWWGG 1 cut(s) 848
TaaI ACNGT 2 cut(s) 397, 719
TaiI ACGT 3 cut(s) 548, 555, 986
TaqI TCGA 4 cut(s) 135, 295, 482, 866
TasI AATT 3 cut(s) 194, 235, 484
TatI WGTACW 1 cut(s) 219
TauI GCSGC 5 cut(s) 60, 389, 419, 503, 574
TfiI GAWTC 4 cut(s) 68, 273, 352, 525
Tru1I TTAA 1 cut(s) 143
Tru9I TTAA 1 cut(s) 143
TseFI GTSAC 2 cut(s) 599, 974
TseI GCWGC 2 cut(s) 389, 761
Tsp45I GTSAC 2 cut(s) 599, 974
TspDTI ATGAA 1 cut(s) 163
TspGWI ACGGA 4 cut(s) 121, 285, 438, 798
TspMI CCCGGG 1 cut(s) 438
XapI RAATTY 2 cut(s) 194, 484
XbaI TCTAGA 1 cut(s) 556
XceI RCATGY 1 cut(s) 592
XmaI CCCGGG 1 cut(s) 438
XspI CTAG 1 cut(s) 557
ZrmI AGTACT 1 cut(s) 221
Zsp2I ATGCAT 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.