Rh7DG177200

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0005627
Physical Location & Seq
Forward (+)
16312081 .. 16313204
1124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG177200.1

Sequence Viewer

Length: 1032 bp
ATGGCTGCGTTGCCGGACCAGAACGGCGATTCCGCGTCCGGCGGACGGGAGTCGCAGAGGACGATTCCGACGCCGTTTTTGACGAAAACGTATGAGCTCGTGGACGATCCGGAGGCCGACGATTTGATTTCGTGGAGTGAAGGCGGATCGACTTTCATAGTGTGGCGGCCGGCCGAATTTGCCAGAGATATACTCCCCAAGTACTTCAAACACAACAACTTCTCCAGCTTCGTCCGCCAGCTAAACACTTACGGATTTCGGAAGGTTGTTCCGGATCGGTGGGAGTTTGCCAACGACTGCTTCAAGAGAGGGGAGAAAGGACTCCTCCGCGAGATTCAGCGCCGGAAGATCTCTCCGGCGGTGTCCGCTTCCGCCGCGGCGGTTGTGGCGGCGGCGACCGTGACGGCGCCGACACTGCCTCCAGCGACGGCTTCGGTTTCTCCGGCTAACTCCGGCGACGAGCAGGTGATCTCGTCCAACTCGTCGCCGATGGCCGTGCCGGCGCCGACTCCGACGGTGGCGTTGCTGCCGCGAACCAGGAGCTGCAACACCACGGCGGAGATTCTAGAGGAGAACGAGCGGTTGAGGAAAGAGAACATGCAGCTCAGCCAGGAGCTGGCTCAGCTTCGGGGGTTGTGTAACAATATACTTGCAATGATGACCAACTACGCCTCGGGGCAGTTAGCGAGAGGCGGCGGAGCACCGGCCGAGGATAAGCGGCCATATCCGCCGCCTCTTGCGGTGAAAAAATCTCAAGAGGGCGAAAACGCCGCCGTATCAGGCGGCGACAAGGCGGAGTACTCGGCGGGGGAAGAGGATCAGGAGGAGGAAACGCCGCCGCTGCTTCCGATTCAGACGCCGAAGCTGTTTGGGGTTTCAATCGGGGTGAAGCGCGTGAGGAGAGACGAGGGGGAGGAGGAGGAGGATCATGAGCGTCAAAACCAAGGGTGTGATGAGGTGGTGAAGCCGGAGCCGTCGGATGGGAGCTCCAAGCACGATAAGGAGGCTACGTGGCTGGAGCTGAGGAAGTGA

Protein Analysis

343

Amino Acids

37.49

Weight (kDa)

5.06

Isoelectric Point (pI)

57.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 26 - 115 5.9e-31 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 454
Acc36I ACCTGC 1 cut(s) 454
AccB1I GGYRCC 2 cut(s) 406, 502
AccB7I CCANNNNNTGG 1 cut(s) 616
AccBSI CCGCTC 1 cut(s) 580
AccII CGCG 5 cut(s) 35, 330, 377, 532, 894
AccIII TCCGGA 2 cut(s) 109, 271
AclWI GGATC 5 cut(s) 101, 154, 282, 825, 933
AcoI YGGCCR 5 cut(s) 167, 171, 492, 705, 719
AcsI RAATTY 1 cut(s) 176
AcyI GRCGYC 4 cut(s) 71, 407, 503, 857
AfaI GTAC 2 cut(s) 203, 800
AfiI CCNNNNNNNGG 3 cut(s) 45, 616, 980
AgsI TTSAA 3 cut(s) 208, 304, 879
AjnI CCWGG 2 cut(s) 536, 609
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AloI GAACNNNNNNTCC 2 cut(s) 14, 46
Alw21I GWGCWC 3 cut(s) 99, 703, 989
Alw26I GTCTC 1 cut(s) 897
AlwI GGATC 5 cut(s) 101, 154, 282, 825, 933
AlwNI CAGNNNCTG 2 cut(s) 543, 616
Ama87I CYCGRG 1 cut(s) 673
Aor13HI TCCGGA 2 cut(s) 109, 271
AoxI GGCC 6 cut(s) 114, 167, 171, 492, 705, 719
ApeKI GCWGC 5 cut(s) 5, 526, 543, 601, 841
ApoI RAATTY 1 cut(s) 176
ArsI GACNNNNNNTTYG 2 cut(s) 61, 93
AspLEI GCGC 4 cut(s) 342, 409, 505, 894
AspS9I GGNCC 1 cut(s) 16
AsuHPI GGTGA 4 cut(s) 478, 754, 898, 973
AvaI CYCGRG 1 cut(s) 673
AvaII GGWCC 1 cut(s) 16
BanI GGYRCC 2 cut(s) 406, 502
BanII GRGCYC 2 cut(s) 99, 989
BauI CACGAG 1 cut(s) 98
Bbv12I GWGCWC 3 cut(s) 99, 703, 989
BbvCI CCTCAGC 1 cut(s) 1022
BbvI GCAGC 4 cut(s) 513, 530, 613, 828
BccI CCATC 2 cut(s) 484, 974
BceAI ACGGC 8 cut(s) 40, 58, 420, 444, 479, 570, 758, 958
BcgI CGANNNNNNTGC 2 cut(s) 478, 512
BciT130I CCWGG 2 cut(s) 538, 611
BcoDI GTCTC 1 cut(s) 897
BfaI CTAG 1 cut(s) 566
BfoI RGCGCY 3 cut(s) 343, 410, 506
BfuAI ACCTGC 1 cut(s) 454
BglI GCCNNNNNGGC 1 cut(s) 500
BglII AGATCT 1 cut(s) 348
BlpI GCTNAGC 2 cut(s) 605, 621
BmcAI AGTACT 2 cut(s) 203, 800
Bme1390I CCNGG 2 cut(s) 538, 611
Bme18I GGWCC 1 cut(s) 16
BmeT110I CYCGRG 1 cut(s) 673
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 3 cut(s) 408, 504, 972
BmrFI CCNGG 2 cut(s) 538, 611
BoxI GACNNNNGTC 1 cut(s) 49
BplI GAGNNNNNCTC 2 cut(s) 177, 209
BpmI CTGGAG 2 cut(s) 208, 405
Bpu10I CCTNAGC 1 cut(s) 1022
Bpu1102I GCTNAGC 2 cut(s) 605, 621
BpuEI CTTGAG 1 cut(s) 738
BsaAI YACGTR 1 cut(s) 1011
BsaHI GRCGYC 4 cut(s) 71, 407, 503, 857
BsaJI CCNNGG 5 cut(s) 375, 552, 672, 708, 943
BsaWI WCCGGW 2 cut(s) 109, 271
BsaXI ACNNNNNCTCC 6 cut(s) 206, 236, 403, 433, 995, 1025
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 616, 980
Bse118I RCCGGY 3 cut(s) 169, 499, 703
Bse3DI GCAATG 1 cut(s) 660
BseAI TCCGGA 2 cut(s) 109, 271
BseBI CCWGG 2 cut(s) 538, 611
BseDI CCNNGG 5 cut(s) 375, 552, 672, 708, 943
BseGI GGATG 1 cut(s) 985
BseLI CCNNNNNNNGG 3 cut(s) 45, 616, 980
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 3 cut(s) 619, 635, 1013
BseRI GAGGAG 7 cut(s) 314, 584, 839, 913, 929, 932, 935
BseX3I CGGCCG 3 cut(s) 167, 171, 705
BseXI GCAGC 4 cut(s) 513, 530, 613, 828
Bsh1236I CGCG 5 cut(s) 35, 330, 377, 532, 894
Bsh1285I CGRYCG 4 cut(s) 170, 174, 399, 708
BshFI GGCC 6 cut(s) 116, 169, 173, 494, 707, 721
BshNI GGYRCC 2 cut(s) 406, 502
BsiEI CGRYCG 4 cut(s) 170, 174, 399, 708
BsiHKAI GWGCWC 3 cut(s) 99, 703, 989
BsiHKCI CYCGRG 1 cut(s) 673
BslI CCNNNNNNNGG 3 cut(s) 45, 616, 980
BsmAI GTCTC 1 cut(s) 897
BsmBI CGTCTC 1 cut(s) 897
BsnI GGCC 6 cut(s) 116, 169, 173, 494, 707, 721
BsoBI CYCGRG 1 cut(s) 673
Bsp1286I GDGCHC 3 cut(s) 99, 703, 989
Bsp13I TCCGGA 2 cut(s) 109, 271
Bsp143I GATC 7 cut(s) 106, 146, 274, 348, 468, 817, 925
Bsp1720I GCTNAGC 2 cut(s) 605, 621
BspANI GGCC 6 cut(s) 116, 169, 173, 494, 707, 721
BspCNI CTCAG 3 cut(s) 618, 634, 1014
BspEI TCCGGA 2 cut(s) 109, 271
BspFNI CGCG 5 cut(s) 35, 330, 377, 532, 894
BspHI TCATGA 1 cut(s) 928
BspLI GGNNCC 3 cut(s) 408, 504, 972
BspMI ACCTGC 1 cut(s) 454
BspPI GGATC 5 cut(s) 101, 154, 282, 825, 933
BspT107I GGYRCC 2 cut(s) 406, 502
BsrBI CCGCTC 1 cut(s) 580
BsrDI GCAATG 1 cut(s) 660
BsrFI RCCGGY 3 cut(s) 169, 499, 703
BssAI RCCGGY 3 cut(s) 169, 499, 703
BssECI CCNNGG 5 cut(s) 375, 552, 672, 708, 943
BssMI GATC 7 cut(s) 106, 146, 274, 348, 468, 817, 925
BssNI GRCGYC 4 cut(s) 71, 407, 503, 857
BssSI CACGAG 1 cut(s) 98
BssT1I CCWWGG 1 cut(s) 943
Bst2BI CACGAG 1 cut(s) 98
Bst2UI CCWGG 2 cut(s) 538, 611
Bst4CI ACNGT 2 cut(s) 400, 517
Bst6I CTCTTC 1 cut(s) 807
BstACI GRCGYC 4 cut(s) 71, 407, 503, 857
BstBAI YACGTR 1 cut(s) 1011
BstC8I GCNNGC 4 cut(s) 171, 239, 501, 618
BstDEI CTNAG 3 cut(s) 605, 621, 1022
BstDSI CCRYGG 2 cut(s) 375, 552
BstF5I GGATG 1 cut(s) 985
BstFNI CGCG 5 cut(s) 35, 330, 377, 532, 894
BstH2I RGCGCY 3 cut(s) 343, 410, 506
BstHHI GCGC 4 cut(s) 342, 409, 505, 894
BstKTI GATC 7 cut(s) 109, 149, 277, 351, 471, 820, 928
BstMAI GTCTC 1 cut(s) 897
BstMBI GATC 7 cut(s) 106, 146, 274, 348, 468, 817, 925
BstMCI CGRYCG 4 cut(s) 170, 174, 399, 708
BstNI CCWGG 2 cut(s) 538, 611
BstNSI RCATGY 1 cut(s) 601
BstPAI GACNNNNGTC 1 cut(s) 49
BstSCI CCNGG 2 cut(s) 536, 609
BstUI CGCG 5 cut(s) 35, 330, 377, 532, 894
BstV1I GCAGC 4 cut(s) 513, 530, 613, 828
BstX2I RGATCY 1 cut(s) 348
BstYI RGATCY 1 cut(s) 348
BstZI CGGCCG 3 cut(s) 167, 171, 705
BsuRI GGCC 6 cut(s) 116, 169, 173, 494, 707, 721
BtgI CCRYGG 2 cut(s) 375, 552
BtsCI GGATG 1 cut(s) 985
BtsI GCAGTG 1 cut(s) 413
BtsIMutI CAGTG 1 cut(s) 413
BveI ACCTGC 1 cut(s) 454
Cac8I GCNNGC 4 cut(s) 171, 239, 501, 618
CaiI CAGNNNCTG 2 cut(s) 543, 616
CciI TCATGA 1 cut(s) 928
CfoI GCGC 4 cut(s) 342, 409, 505, 894
Cfr10I RCCGGY 3 cut(s) 169, 499, 703
Cfr13I GGNCC 1 cut(s) 16
Cfr42I CCGCGG 1 cut(s) 378
CseI GACGC 4 cut(s) 24, 79, 865, 923
Csp6I GTAC 2 cut(s) 202, 799
CviAII CATG 2 cut(s) 598, 929
CviQI GTAC 2 cut(s) 202, 799
DdeI CTNAG 3 cut(s) 605, 621, 1022
DinI GGCGCC 2 cut(s) 408, 504
DpnI GATC 7 cut(s) 108, 148, 276, 350, 470, 819, 927
DpnII GATC 7 cut(s) 106, 146, 274, 348, 468, 817, 925
EaeI YGGCCR 5 cut(s) 167, 171, 492, 705, 719
EagI CGGCCG 3 cut(s) 167, 171, 705
Eam1104I CTCTTC 1 cut(s) 807
EarI CTCTTC 1 cut(s) 807
EciI GGCGGA 8 cut(s) 57, 159, 224, 361, 572, 711, 717, 809
Ecl136II GAGCTC 2 cut(s) 97, 987
EclXI CGGCCG 3 cut(s) 167, 171, 705
Eco130I CCWWGG 1 cut(s) 943
Eco24I GRGCYC 2 cut(s) 99, 989
Eco47I GGWCC 1 cut(s) 16
Eco52I CGGCCG 3 cut(s) 167, 171, 705
Eco53kI GAGCTC 2 cut(s) 97, 987
Eco88I CYCGRG 1 cut(s) 673
EcoICRI GAGCTC 2 cut(s) 97, 987
EcoRII CCWGG 2 cut(s) 536, 609
EcoT14I CCWWGG 1 cut(s) 943
EcoT38I GRGCYC 2 cut(s) 99, 989
EgeI GGCGCC 2 cut(s) 408, 504
EheI GGCGCC 2 cut(s) 408, 504
ErhI CCWWGG 1 cut(s) 943
Esp3I CGTCTC 1 cut(s) 897
FaeI CATG 2 cut(s) 601, 932
FaiI YATR 7 cut(s) 93, 158, 191, 599, 647, 724, 930
FatI CATG 2 cut(s) 597, 928
FauI CCCGC 1 cut(s) 799
FokI GGATG 1 cut(s) 992
FriOI GRGCYC 2 cut(s) 99, 989
FseI GGCCGGCC 1 cut(s) 173
FspBI CTAG 1 cut(s) 566
GlaI GCGC 4 cut(s) 341, 408, 504, 893
GsuI CTGGAG 2 cut(s) 208, 405
HaeII RGCGCY 3 cut(s) 343, 410, 506
HaeIII GGCC 6 cut(s) 116, 169, 173, 494, 707, 721
HgaI GACGC 4 cut(s) 24, 79, 865, 923
HhaI GCGC 4 cut(s) 342, 409, 505, 894
Hin1I GRCGYC 4 cut(s) 71, 407, 503, 857
Hin1II CATG 2 cut(s) 601, 932
Hin6I GCGC 4 cut(s) 340, 407, 503, 892
HinP1I GCGC 4 cut(s) 340, 407, 503, 892
HinfI GANTC 8 cut(s) 29, 50, 64, 321, 334, 508, 562, 850
HphI GGTGA 4 cut(s) 478, 754, 898, 973
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 6 cut(s) 69, 261, 513, 849, 855, 979
Hpy188III TCNNGA 7 cut(s) 110, 272, 304, 566, 755, 821, 929
Hpy8I GTNNAC 1 cut(s) 103
Hpy99I CGWCG 7 cut(s) 73, 122, 430, 461, 487, 517, 979
HpyAV CCTTC 2 cut(s) 134, 256
HpyCH4III ACNGT 2 cut(s) 400, 517
HpyCH4IV ACGT 2 cut(s) 89, 1010
HpyCH4V TGCA 3 cut(s) 546, 601, 653
HpyF3I CTNAG 3 cut(s) 605, 621, 1022
HpySE526I ACGT 2 cut(s) 89, 1010
Hsp92I GRCGYC 4 cut(s) 71, 407, 503, 857
Hsp92II CATG 2 cut(s) 601, 932
HspAI GCGC 4 cut(s) 340, 407, 503, 892
KasI GGCGCC 2 cut(s) 406, 502
Kpn2I TCCGGA 2 cut(s) 109, 271
KroI GCCGGC 2 cut(s) 169, 499
KroNI GCCGGC 2 cut(s) 171, 501
KspI CCGCGG 1 cut(s) 378
Kzo9I GATC 7 cut(s) 106, 146, 274, 348, 468, 817, 925
LmnI GCTCC 7 cut(s) 540, 613, 698, 970, 984, 992, 1018
Lsp1109I GCAGC 4 cut(s) 513, 530, 613, 828
MaeI CTAG 1 cut(s) 566
MaeII ACGT 2 cut(s) 89, 1010
MaeIII GTNAC 2 cut(s) 400, 638
MalI GATC 7 cut(s) 108, 148, 276, 350, 470, 819, 927
MbiI CCGCTC 1 cut(s) 580
MboI GATC 7 cut(s) 106, 146, 274, 348, 468, 817, 925
MboII GAAGA 2 cut(s) 358, 824
MflI RGATCY 1 cut(s) 348
MhlI GDGCHC 3 cut(s) 99, 703, 989
MluCI AATT 1 cut(s) 176
Mly113I GGCGCC 2 cut(s) 407, 503
MlyI GAGTC 3 cut(s) 59, 315, 502
MmeI TCCRAC 4 cut(s) 92, 501, 536, 957
MroI TCCGGA 2 cut(s) 109, 271
MroNI GCCGGC 2 cut(s) 169, 499
MspA1I CMGCKG 2 cut(s) 377, 841
MspR9I CCNGG 2 cut(s) 538, 611
MvaI CCWGG 2 cut(s) 538, 611
MvnI CGCG 5 cut(s) 35, 330, 377, 532, 894
NaeI GCCGGC 2 cut(s) 171, 501
NarI GGCGCC 2 cut(s) 407, 503
NdeII GATC 7 cut(s) 106, 146, 274, 348, 468, 817, 925
NgoMIV GCCGGC 2 cut(s) 169, 499
NlaIII CATG 2 cut(s) 601, 932
NlaIV GGNNCC 3 cut(s) 408, 504, 972
NmeAIII GCCGAG 2 cut(s) 733, 782
NmuCI GTSAC 1 cut(s) 400
NspI RCATGY 1 cut(s) 601
PagI TCATGA 1 cut(s) 928
PaqCI CACCTGC 1 cut(s) 454
PcsI WCGNNNNNNNCGW 2 cut(s) 59, 479
PdiI GCCGGC 2 cut(s) 171, 501
PfeI GAWTC 5 cut(s) 29, 64, 334, 562, 850
PflMI CCANNNNNTGG 1 cut(s) 616
PleI GAGTC 3 cut(s) 58, 315, 502
PluTI GGCGCC 2 cut(s) 410, 506
PpsI GAGTC 3 cut(s) 58, 315, 502
Ppu21I YACGTR 1 cut(s) 1011
PshAI GACNNNNGTC 1 cut(s) 49
Psp124BI GAGCTC 2 cut(s) 99, 989
Psp6I CCWGG 2 cut(s) 536, 609
PspGI CCWGG 2 cut(s) 536, 609
PspN4I GGNNCC 3 cut(s) 408, 504, 972
PspPI GGNCC 1 cut(s) 16
PstNI CAGNNNCTG 2 cut(s) 543, 616
PsuI RGATCY 1 cut(s) 348
RigI GGCCGGCC 1 cut(s) 173
RsaI GTAC 2 cut(s) 203, 800
RsaNI GTAC 2 cut(s) 202, 799
SacI GAGCTC 2 cut(s) 99, 989
SacII CCGCGG 1 cut(s) 378
Sau3AI GATC 7 cut(s) 106, 146, 274, 348, 468, 817, 925
Sau96I GGNCC 1 cut(s) 16
ScaI AGTACT 2 cut(s) 203, 800
SchI GAGTC 3 cut(s) 59, 315, 502
ScrFI CCNGG 2 cut(s) 538, 611
SduI GDGCHC 3 cut(s) 99, 703, 989
SfoI GGCGCC 2 cut(s) 408, 504
Sfr303I CCGCGG 1 cut(s) 378
SgrBI CCGCGG 1 cut(s) 378
SinI GGWCC 1 cut(s) 16
SmlI CTYRAG 1 cut(s) 753
SmoI CTYRAG 1 cut(s) 753
Sse9I AATT 1 cut(s) 176
SspDI GGCGCC 2 cut(s) 406, 502
SspMI CTAG 1 cut(s) 566
SstI GAGCTC 2 cut(s) 99, 989
StyD4I CCNGG 2 cut(s) 536, 609
StyI CCWWGG 1 cut(s) 943
TaaI ACNGT 2 cut(s) 400, 517
TaiI ACGT 2 cut(s) 92, 1013
TaqI TCGA 1 cut(s) 149
TasI AATT 1 cut(s) 176
TatI WGTACW 2 cut(s) 201, 798
TfiI GAWTC 5 cut(s) 29, 64, 334, 562, 850
TscAI CASTG 1 cut(s) 420
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 5 cut(s) 5, 526, 543, 601, 841
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 1 cut(s) 145
TspGWI ACGGA 1 cut(s) 267
TspRI CASTG 1 cut(s) 420
Van91I CCANNNNNTGG 1 cut(s) 616
VpaK11BI GGWCC 1 cut(s) 16
XapI RAATTY 1 cut(s) 176
XbaI TCTAGA 1 cut(s) 565
XceI RCATGY 1 cut(s) 601
XspI CTAG 1 cut(s) 566
ZrmI AGTACT 2 cut(s) 203, 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.