RchiOBHm_Chr7g0198941

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0005627
Physical Location & Seq
Forward (+)
16963984 .. 16966141
2158 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17801

Sequence Viewer

Length: 1041 bp
ATGGCTGCGTTGCCGGACCAGAACGGCGATTCGGCGTCCGGCGGACGGGAGTCGCAGAGGACGATTCCGACGCCGTTTTTGACGAAAACGTATGAGCTCGTGGACGATCCGGAGGCCGACGATTTGATTTCGTGGAGTGAAGGCGGATCGACTTTCATAGTGTGGCGGCCGGCCGAATTTGCCAGAGATATACTCCCCAAGTACTTCAAACACAACAACTTCTCCAGCTTCGTCCGCCAGCTAAACACTTACGGATTTCGGAAGGTTGTTCCGGATCGGTGGGAGTTTGCCAACGACTGCTTCAAGAGAGGGGAGAAAGGACTCCTCCGCGAGATTCAACGCCGGAAGATCTCTCCGGCGGTGTCAGCTTCCGCCGCGGCGGTTGTGGCGGCGGCGACCGTGACGGCGGCGACACTGCCTCCAGCGACGGCTTCGGTTTCTCCGGCAAACTCCGGCGACGAGCAGGTGATCTCGTCCAACTCGTCGCCGATGGCCGTGCCGGCGCCGACTCCGACGGTGGCGTTGCTGCCTCGAACCAGGAGCTGCAACACCACGGCGGAGATTCTAGAGGAGAACGAGCGGTTGAGGAAAGAGAACATGCAGCTCAGCCAGGAGCTGGCTCAGCTTCGGGGGTTGTGTAACAATATACTTGCAATGATGACCAACTACGCCTCGGGGCAGTTAGCGAGAGGCGGCGGAGCAGCGGCCGAGGATAAGCGGCCATATCCGCCGCCTCCGGTGAAAAAATCTCAAGAGGGCCAAAAAGCCGCCGTATCAGGCGGCGACAAGGCGGAGTACTCGGCGGGGGAAGAGGAGATGGAGGAGGATCAGGAGGAGGAAACGCCGCCGCTGCTTCCGATTCAGACGCCGAAGCTGTTTGGGGTTTCAATCGGGGTGAAGCGCGTGAGGAGAGACGAGGGGGAGGAGGAGGAGGATCATGAGCGTCAAAACCAAGGGTGTGATGAGGTGGTGAAGCCGGAGCCGTCGGATGGGAGCTCCAAGCACGATAAGGAGGCTACGTGGCTGGAGCTGAGGAAGTGA

Protein Analysis

346

Amino Acids

37.89

Weight (kDa)

5.03

Isoelectric Point (pI)

58.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 26 - 115 6e-31 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 454
Acc36I ACCTGC 1 cut(s) 454
AccB1I GGYRCC 1 cut(s) 502
AccB7I CCANNNNNTGG 1 cut(s) 616
AccBSI CCGCTC 1 cut(s) 580
AccII CGCG 3 cut(s) 330, 377, 903
AccIII TCCGGA 2 cut(s) 109, 271
AclWI GGATC 5 cut(s) 101, 154, 282, 834, 942
AcoI YGGCCR 5 cut(s) 167, 171, 492, 705, 719
AcsI RAATTY 1 cut(s) 176
AcyI GRCGYC 4 cut(s) 35, 71, 503, 866
AfaI GTAC 2 cut(s) 203, 797
AfiI CCNNNNNNNGG 3 cut(s) 45, 616, 989
AgsI TTSAA 4 cut(s) 208, 304, 338, 888
AjnI CCWGG 2 cut(s) 536, 609
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
Alw21I GWGCWC 2 cut(s) 99, 998
Alw26I GTCTC 1 cut(s) 906
AlwI GGATC 5 cut(s) 101, 154, 282, 834, 942
AlwNI CAGNNNCTG 2 cut(s) 543, 616
Ama87I CYCGRG 1 cut(s) 673
Aor13HI TCCGGA 2 cut(s) 109, 271
AoxI GGCC 7 cut(s) 114, 167, 171, 492, 705, 719, 757
ApeKI GCWGC 6 cut(s) 5, 526, 543, 601, 701, 850
ApoI RAATTY 1 cut(s) 176
ArsI GACNNNNNNTTYG 2 cut(s) 61, 93
AspLEI GCGC 2 cut(s) 505, 903
AspS9I GGNCC 2 cut(s) 16, 757
AsuHPI GGTGA 4 cut(s) 478, 751, 907, 982
AvaI CYCGRG 1 cut(s) 673
AvaII GGWCC 1 cut(s) 16
BanI GGYRCC 1 cut(s) 502
BanII GRGCYC 2 cut(s) 99, 998
BauI CACGAG 1 cut(s) 98
Bbv12I GWGCWC 2 cut(s) 99, 998
BbvCI CCTCAGC 1 cut(s) 1031
BbvI GCAGC 5 cut(s) 513, 530, 613, 713, 837
BccI CCATC 3 cut(s) 484, 811, 983
BceAI ACGGC 8 cut(s) 40, 58, 420, 444, 479, 570, 755, 967
BcgI CGANNNNNNTGC 2 cut(s) 478, 512
BciT130I CCWGG 2 cut(s) 538, 611
BcoDI GTCTC 1 cut(s) 906
BfaI CTAG 1 cut(s) 566
BfoI RGCGCY 1 cut(s) 506
BfuAI ACCTGC 1 cut(s) 454
BglI GCCNNNNNGGC 1 cut(s) 500
BglII AGATCT 1 cut(s) 348
BlpI GCTNAGC 2 cut(s) 605, 621
BmcAI AGTACT 2 cut(s) 203, 797
Bme1390I CCNGG 2 cut(s) 538, 611
Bme18I GGWCC 1 cut(s) 16
BmeT110I CYCGRG 1 cut(s) 673
BmgT120I GGNCC 2 cut(s) 16, 757
BmiI GGNNCC 2 cut(s) 504, 981
BmrFI CCNGG 2 cut(s) 538, 611
BoxI GACNNNNGTC 1 cut(s) 49
BplI GAGNNNNNCTC 2 cut(s) 177, 209
BpmI CTGGAG 2 cut(s) 208, 405
Bpu10I CCTNAGC 1 cut(s) 1031
Bpu1102I GCTNAGC 2 cut(s) 605, 621
BpuEI CTTGAG 1 cut(s) 735
BsaAI YACGTR 1 cut(s) 1020
BsaHI GRCGYC 4 cut(s) 35, 71, 503, 866
BsaJI CCNNGG 5 cut(s) 375, 552, 672, 708, 952
BsaWI WCCGGW 3 cut(s) 109, 271, 736
BsaXI ACNNNNNCTCC 6 cut(s) 206, 236, 403, 433, 1004, 1034
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 616, 989
Bse118I RCCGGY 2 cut(s) 169, 499
Bse3DI GCAATG 1 cut(s) 660
BseAI TCCGGA 2 cut(s) 109, 271
BseBI CCWGG 2 cut(s) 538, 611
BseDI CCNNGG 5 cut(s) 375, 552, 672, 708, 952
BseGI GGATG 1 cut(s) 994
BseLI CCNNNNNNNGG 3 cut(s) 45, 616, 989
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 3 cut(s) 619, 635, 1022
BseRI GAGGAG 9 cut(s) 314, 584, 827, 836, 848, 922, 938, 941, 944
BseX3I CGGCCG 3 cut(s) 167, 171, 705
BseXI GCAGC 5 cut(s) 513, 530, 613, 713, 837
Bsh1236I CGCG 3 cut(s) 330, 377, 903
Bsh1285I CGRYCG 4 cut(s) 170, 174, 399, 708
BshFI GGCC 7 cut(s) 116, 169, 173, 494, 707, 721, 759
BshNI GGYRCC 1 cut(s) 502
BsiEI CGRYCG 4 cut(s) 170, 174, 399, 708
BsiHKAI GWGCWC 2 cut(s) 99, 998
BsiHKCI CYCGRG 1 cut(s) 673
BslI CCNNNNNNNGG 3 cut(s) 45, 616, 989
BsmAI GTCTC 1 cut(s) 906
BsmBI CGTCTC 1 cut(s) 906
BsnI GGCC 7 cut(s) 116, 169, 173, 494, 707, 721, 759
BsoBI CYCGRG 1 cut(s) 673
Bsp1286I GDGCHC 2 cut(s) 99, 998
Bsp13I TCCGGA 2 cut(s) 109, 271
Bsp143I GATC 7 cut(s) 106, 146, 274, 348, 468, 826, 934
Bsp1720I GCTNAGC 2 cut(s) 605, 621
BspANI GGCC 7 cut(s) 116, 169, 173, 494, 707, 721, 759
BspCNI CTCAG 3 cut(s) 618, 634, 1023
BspEI TCCGGA 2 cut(s) 109, 271
BspFNI CGCG 3 cut(s) 330, 377, 903
BspHI TCATGA 1 cut(s) 937
BspLI GGNNCC 2 cut(s) 504, 981
BspMI ACCTGC 1 cut(s) 454
BspPI GGATC 5 cut(s) 101, 154, 282, 834, 942
BspT107I GGYRCC 1 cut(s) 502
BsrBI CCGCTC 1 cut(s) 580
BsrDI GCAATG 1 cut(s) 660
BsrFI RCCGGY 2 cut(s) 169, 499
BssAI RCCGGY 2 cut(s) 169, 499
BssECI CCNNGG 5 cut(s) 375, 552, 672, 708, 952
BssMI GATC 7 cut(s) 106, 146, 274, 348, 468, 826, 934
BssNI GRCGYC 4 cut(s) 35, 71, 503, 866
BssSI CACGAG 1 cut(s) 98
BssT1I CCWWGG 1 cut(s) 952
Bst2BI CACGAG 1 cut(s) 98
Bst2UI CCWGG 2 cut(s) 538, 611
Bst4CI ACNGT 2 cut(s) 400, 517
Bst6I CTCTTC 1 cut(s) 804
BstACI GRCGYC 4 cut(s) 35, 71, 503, 866
BstBAI YACGTR 1 cut(s) 1020
BstC8I GCNNGC 4 cut(s) 171, 239, 501, 618
BstDEI CTNAG 3 cut(s) 605, 621, 1031
BstDSI CCRYGG 2 cut(s) 375, 552
BstF5I GGATG 1 cut(s) 994
BstFNI CGCG 3 cut(s) 330, 377, 903
BstH2I RGCGCY 1 cut(s) 506
BstHHI GCGC 2 cut(s) 505, 903
BstKTI GATC 7 cut(s) 109, 149, 277, 351, 471, 829, 937
BstMAI GTCTC 1 cut(s) 906
BstMBI GATC 7 cut(s) 106, 146, 274, 348, 468, 826, 934
BstMCI CGRYCG 4 cut(s) 170, 174, 399, 708
BstMWI GCNNNNNNNGC 9 cut(s) 179, 234, 365, 374, 386, 500, 622, 727, 850
BstNI CCWGG 2 cut(s) 538, 611
BstNSI RCATGY 1 cut(s) 601
BstPAI GACNNNNGTC 1 cut(s) 49
BstSCI CCNGG 2 cut(s) 536, 609
BstUI CGCG 3 cut(s) 330, 377, 903
BstV1I GCAGC 5 cut(s) 513, 530, 613, 713, 837
BstX2I RGATCY 1 cut(s) 348
BstYI RGATCY 1 cut(s) 348
BstZI CGGCCG 3 cut(s) 167, 171, 705
BsuRI GGCC 7 cut(s) 116, 169, 173, 494, 707, 721, 759
BtgI CCRYGG 2 cut(s) 375, 552
BtsCI GGATG 1 cut(s) 994
BtsI GCAGTG 1 cut(s) 413
BtsIMutI CAGTG 1 cut(s) 413
BveI ACCTGC 1 cut(s) 454
Cac8I GCNNGC 4 cut(s) 171, 239, 501, 618
CaiI CAGNNNCTG 2 cut(s) 543, 616
CciI TCATGA 1 cut(s) 937
CfoI GCGC 2 cut(s) 505, 903
Cfr10I RCCGGY 2 cut(s) 169, 499
Cfr13I GGNCC 2 cut(s) 16, 757
Cfr42I CCGCGG 1 cut(s) 378
CseI GACGC 4 cut(s) 24, 79, 874, 932
Csp6I GTAC 2 cut(s) 202, 796
CviAII CATG 2 cut(s) 598, 938
CviQI GTAC 2 cut(s) 202, 796
DdeI CTNAG 3 cut(s) 605, 621, 1031
DinI GGCGCC 1 cut(s) 504
DpnI GATC 7 cut(s) 108, 148, 276, 350, 470, 828, 936
DpnII GATC 7 cut(s) 106, 146, 274, 348, 468, 826, 934
EaeI YGGCCR 5 cut(s) 167, 171, 492, 705, 719
EagI CGGCCG 3 cut(s) 167, 171, 705
Eam1104I CTCTTC 1 cut(s) 804
EarI CTCTTC 1 cut(s) 804
EciI GGCGGA 8 cut(s) 57, 159, 224, 361, 572, 711, 717, 806
Ecl136II GAGCTC 2 cut(s) 97, 996
EclXI CGGCCG 3 cut(s) 167, 171, 705
Eco130I CCWWGG 1 cut(s) 952
Eco24I GRGCYC 2 cut(s) 99, 998
Eco47I GGWCC 1 cut(s) 16
Eco52I CGGCCG 3 cut(s) 167, 171, 705
Eco53kI GAGCTC 2 cut(s) 97, 996
Eco88I CYCGRG 1 cut(s) 673
EcoICRI GAGCTC 2 cut(s) 97, 996
EcoRII CCWGG 2 cut(s) 536, 609
EcoT14I CCWWGG 1 cut(s) 952
EcoT38I GRGCYC 2 cut(s) 99, 998
EgeI GGCGCC 1 cut(s) 504
EheI GGCGCC 1 cut(s) 504
ErhI CCWWGG 1 cut(s) 952
Esp3I CGTCTC 1 cut(s) 906
FaeI CATG 2 cut(s) 601, 941
FaiI YATR 7 cut(s) 93, 158, 191, 599, 647, 724, 939
FatI CATG 2 cut(s) 597, 937
FauI CCCGC 1 cut(s) 796
FokI GGATG 1 cut(s) 1001
FriOI GRGCYC 2 cut(s) 99, 998
FseI GGCCGGCC 1 cut(s) 173
FspBI CTAG 1 cut(s) 566
GlaI GCGC 2 cut(s) 504, 902
GsuI CTGGAG 2 cut(s) 208, 405
HaeII RGCGCY 1 cut(s) 506
HaeIII GGCC 7 cut(s) 116, 169, 173, 494, 707, 721, 759
HgaI GACGC 4 cut(s) 24, 79, 874, 932
HhaI GCGC 2 cut(s) 505, 903
Hin1I GRCGYC 4 cut(s) 35, 71, 503, 866
Hin1II CATG 2 cut(s) 601, 941
Hin6I GCGC 2 cut(s) 503, 901
HinP1I GCGC 2 cut(s) 503, 901
HinfI GANTC 8 cut(s) 29, 50, 64, 321, 334, 508, 562, 859
HphI GGTGA 4 cut(s) 478, 751, 907, 982
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 6 cut(s) 69, 261, 513, 858, 864, 988
Hpy188III TCNNGA 7 cut(s) 110, 272, 304, 566, 752, 830, 938
Hpy8I GTNNAC 1 cut(s) 103
Hpy99I CGWCG 7 cut(s) 73, 122, 430, 461, 487, 517, 988
HpyAV CCTTC 2 cut(s) 134, 256
HpyCH4III ACNGT 2 cut(s) 400, 517
HpyCH4IV ACGT 2 cut(s) 89, 1019
HpyCH4V TGCA 3 cut(s) 546, 601, 653
HpyF10VI GCNNNNNNNGC 9 cut(s) 179, 234, 365, 374, 386, 500, 622, 727, 850
HpyF3I CTNAG 3 cut(s) 605, 621, 1031
HpySE526I ACGT 2 cut(s) 89, 1019
Hsp92I GRCGYC 4 cut(s) 35, 71, 503, 866
Hsp92II CATG 2 cut(s) 601, 941
HspAI GCGC 2 cut(s) 503, 901
KasI GGCGCC 1 cut(s) 502
Kpn2I TCCGGA 2 cut(s) 109, 271
KroI GCCGGC 2 cut(s) 169, 499
KroNI GCCGGC 2 cut(s) 171, 501
KspI CCGCGG 1 cut(s) 378
Kzo9I GATC 7 cut(s) 106, 146, 274, 348, 468, 826, 934
LmnI GCTCC 7 cut(s) 540, 613, 698, 979, 993, 1001, 1027
Lsp1109I GCAGC 5 cut(s) 513, 530, 613, 713, 837
MaeI CTAG 1 cut(s) 566
MaeII ACGT 2 cut(s) 89, 1019
MaeIII GTNAC 2 cut(s) 400, 638
MalI GATC 7 cut(s) 108, 148, 276, 350, 470, 828, 936
MbiI CCGCTC 1 cut(s) 580
MboI GATC 7 cut(s) 106, 146, 274, 348, 468, 826, 934
MboII GAAGA 2 cut(s) 358, 821
MflI RGATCY 1 cut(s) 348
MhlI GDGCHC 2 cut(s) 99, 998
MluCI AATT 1 cut(s) 176
Mly113I GGCGCC 1 cut(s) 503
MlyI GAGTC 3 cut(s) 59, 315, 502
MmeI TCCRAC 4 cut(s) 92, 501, 536, 966
MroI TCCGGA 2 cut(s) 109, 271
MroNI GCCGGC 2 cut(s) 169, 499
MspA1I CMGCKG 3 cut(s) 377, 704, 850
MspR9I CCNGG 2 cut(s) 538, 611
MvaI CCWGG 2 cut(s) 538, 611
MvnI CGCG 3 cut(s) 330, 377, 903
MwoI GCNNNNNNNGC 9 cut(s) 179, 234, 365, 374, 386, 500, 622, 727, 850
NaeI GCCGGC 2 cut(s) 171, 501
NarI GGCGCC 1 cut(s) 503
NdeII GATC 7 cut(s) 106, 146, 274, 348, 468, 826, 934
NgoMIV GCCGGC 2 cut(s) 169, 499
NlaIII CATG 2 cut(s) 601, 941
NlaIV GGNNCC 2 cut(s) 504, 981
NmeAIII GCCGAG 2 cut(s) 733, 779
NmuCI GTSAC 1 cut(s) 400
NspI RCATGY 1 cut(s) 601
PagI TCATGA 1 cut(s) 937
PaqCI CACCTGC 1 cut(s) 454
PcsI WCGNNNNNNNCGW 2 cut(s) 59, 479
PdiI GCCGGC 2 cut(s) 171, 501
PfeI GAWTC 5 cut(s) 29, 64, 334, 562, 859
PflMI CCANNNNNTGG 1 cut(s) 616
PleI GAGTC 3 cut(s) 58, 315, 502
PluTI GGCGCC 1 cut(s) 506
PpsI GAGTC 3 cut(s) 58, 315, 502
Ppu21I YACGTR 1 cut(s) 1020
PshAI GACNNNNGTC 1 cut(s) 49
Psp124BI GAGCTC 2 cut(s) 99, 998
Psp6I CCWGG 2 cut(s) 536, 609
PspGI CCWGG 2 cut(s) 536, 609
PspN4I GGNNCC 2 cut(s) 504, 981
PspPI GGNCC 2 cut(s) 16, 757
PstNI CAGNNNCTG 2 cut(s) 543, 616
PsuI RGATCY 1 cut(s) 348
RigI GGCCGGCC 1 cut(s) 173
RsaI GTAC 2 cut(s) 203, 797
RsaNI GTAC 2 cut(s) 202, 796
SacI GAGCTC 2 cut(s) 99, 998
SacII CCGCGG 1 cut(s) 378
Sau3AI GATC 7 cut(s) 106, 146, 274, 348, 468, 826, 934
Sau96I GGNCC 2 cut(s) 16, 757
ScaI AGTACT 2 cut(s) 203, 797
SchI GAGTC 3 cut(s) 59, 315, 502
ScrFI CCNGG 2 cut(s) 538, 611
SduI GDGCHC 2 cut(s) 99, 998
SfoI GGCGCC 1 cut(s) 504
Sfr303I CCGCGG 1 cut(s) 378
SgrBI CCGCGG 1 cut(s) 378
SinI GGWCC 1 cut(s) 16
SmlI CTYRAG 1 cut(s) 750
SmoI CTYRAG 1 cut(s) 750
Sse9I AATT 1 cut(s) 176
SspDI GGCGCC 1 cut(s) 502
SspMI CTAG 1 cut(s) 566
SstI GAGCTC 2 cut(s) 99, 998
StyD4I CCNGG 2 cut(s) 536, 609
StyI CCWWGG 1 cut(s) 952
TaaI ACNGT 2 cut(s) 400, 517
TaiI ACGT 2 cut(s) 92, 1022
TaqI TCGA 2 cut(s) 149, 532
TasI AATT 1 cut(s) 176
TatI WGTACW 2 cut(s) 201, 795
TfiI GAWTC 5 cut(s) 29, 64, 334, 562, 859
TscAI CASTG 1 cut(s) 420
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 6 cut(s) 5, 526, 543, 601, 701, 850
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 1 cut(s) 145
TspGWI ACGGA 1 cut(s) 267
TspRI CASTG 1 cut(s) 420
Van91I CCANNNNNTGG 1 cut(s) 616
VpaK11BI GGWCC 1 cut(s) 16
XapI RAATTY 1 cut(s) 176
XbaI TCTAGA 1 cut(s) 565
XceI RCATGY 1 cut(s) 601
XspI CTAG 1 cut(s) 566
ZrmI AGTACT 2 cut(s) 203, 797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.