Rroxscaffold_3G00257300

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005627
Physical Location & Seq
Reverse (-)
51699079 .. 51700210
1132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00257300.1

Sequence Viewer

Length: 1044 bp
ATGGCTGCGTTGCCGGACCAGAACGGCGATTCGGCGTCCGGCGGACGGGAGTCGCAGAGGACGATTCCGACGCCGTTTTTGACGAAAACGTATGAGCTCGTGGACGATCCGGAGGCCGACGATTTGATTTCGTGGAGCGAAGGCGGATCGACTTTCATAGTGTGGCGGCCGGCCGAATTCGCCAGAGATATACTCCCAAAGTACTTCAAACACAACAACTTCTCCAGCTTCGTCCGCCAGCTAAACACTTATGTGAGGATTTCGGAAGGTTGTTCCCGATCGGTGGGAGTTTGCCAACGACTGCTTCAAGAGAGGGGAGAAAGGACTCCTCCGCGAGATTCAGCGCCGGAAGATCTCTCCGGCGGTGTCCGCTTCCGCCGCGGCGGTTGTGGCGGCGGCGACCGTTACGGCGGCGACACTGCCTCCAGCGACGGTTTCGGTTTCTCCGGCAAACTCCGGCGACGAGCAGGTGATCTCGTCCAACTCGTCGCCGATGGCCGTGCCGGCGCCGACTCCGACGGTGGCGTTGCTGCCGCGAACCAGGAGCTGCAACACCACGGCGGAGATTCTAGAGGAGAACGAGCGGTTGAGGAAAGAGAACATGCAGCTCAGCCTGGAGCTGGCTCAGCTTCGGGGTTGTGTAACAATATACTAGCAATGATGACCAACTACGCCTCGGGGCAGTTAGCGAGAGGCGGAGGAGCAGCGGCCGAGGATAAGCGGCCACATCCGCCGCCTCTTGCGGTGAAAAAATCTCAAGAGGGCGAAAACGCCGCCGTATCAGGCGGCGACAAGGCGGAGTACTCGGCGGGGGAAGAGGAGATGGAGGAGGATCAGGAGGAGGAAACGCAGCCGCGGCTTCCGATTCAGACGCCGAAGCTGTTTGGGGTTTCAATCGGGGTGAAGCGCGTGAGGAGAGACGAGGAGGAGGAGGAGGATCATGAGCGTCAAAACCAAGGGTGTGATGAGGTGGTGAAGCCGGAGCCGTCCGATGGGAGCTCCAAGCACGATAAGGAGGCTACGTGGCTGGAGCTGAGGAAGTGA

Protein Analysis

347

Amino Acids

37.51

Weight (kDa)

4.99

Isoelectric Point (pI)

52.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 26 - 84 5.7e-21 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 458
Acc36I ACCTGC 1 cut(s) 458
AccB1I GGYRCC 1 cut(s) 506
AccBSI CCGCTC 1 cut(s) 584
AccII CGCG 5 cut(s) 334, 381, 536, 856, 909
AccIII TCCGGA 1 cut(s) 109
AclWI GGATC 4 cut(s) 101, 154, 840, 945
AcoI YGGCCR 5 cut(s) 167, 171, 496, 708, 722
AcsI RAATTY 1 cut(s) 176
AcyI GRCGYC 4 cut(s) 35, 71, 507, 872
AfaI GTAC 2 cut(s) 203, 803
AfiI CCNNNNNNNGG 4 cut(s) 45, 283, 620, 992
AgsI TTSAA 3 cut(s) 208, 308, 894
AjnI CCWGG 2 cut(s) 540, 613
AjuI GAANNNNNNNTTGG 2 cut(s) 288, 320
AleI CACNNNNGTG 1 cut(s) 251
Alw21I GWGCWC 2 cut(s) 99, 1001
Alw26I GTCTC 1 cut(s) 912
AlwI GGATC 4 cut(s) 101, 154, 840, 945
AlwNI CAGNNNCTG 1 cut(s) 547
Ama87I CYCGRG 1 cut(s) 676
Aor13HI TCCGGA 1 cut(s) 109
AoxI GGCC 6 cut(s) 114, 167, 171, 496, 708, 722
ApeKI GCWGC 6 cut(s) 5, 530, 547, 605, 704, 850
ApoI RAATTY 1 cut(s) 176
ArsI GACNNNNNNTTYG 2 cut(s) 61, 93
AspLEI GCGC 3 cut(s) 346, 509, 909
AspS9I GGNCC 1 cut(s) 16
AsuHPI GGTGA 4 cut(s) 482, 757, 913, 985
AvaI CYCGRG 1 cut(s) 676
AvaII GGWCC 1 cut(s) 16
BanI GGYRCC 1 cut(s) 506
BanII GRGCYC 2 cut(s) 99, 1001
BauI CACGAG 1 cut(s) 98
Bbv12I GWGCWC 2 cut(s) 99, 1001
BbvCI CCTCAGC 1 cut(s) 1034
BbvI GCAGC 5 cut(s) 517, 534, 617, 716, 862
BccI CCATC 3 cut(s) 488, 817, 986
BceAI ACGGC 7 cut(s) 40, 58, 424, 483, 574, 761, 970
BcgI CGANNNNNNTGC 2 cut(s) 482, 516
BciT130I CCWGG 2 cut(s) 542, 615
BcoDI GTCTC 1 cut(s) 912
BfaI CTAG 2 cut(s) 570, 653
BfoI RGCGCY 2 cut(s) 347, 510
BfuAI ACCTGC 1 cut(s) 458
BglI GCCNNNNNGGC 1 cut(s) 504
BglII AGATCT 1 cut(s) 352
BlpI GCTNAGC 2 cut(s) 609, 625
BmcAI AGTACT 2 cut(s) 203, 803
Bme1390I CCNGG 2 cut(s) 542, 615
Bme18I GGWCC 1 cut(s) 16
BmeT110I CYCGRG 1 cut(s) 676
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 2 cut(s) 508, 984
BmrFI CCNGG 2 cut(s) 542, 615
BoxI GACNNNNGTC 1 cut(s) 49
BplI GAGNNNNNCTC 2 cut(s) 177, 209
BpmI CTGGAG 3 cut(s) 208, 409, 636
Bpu10I CCTNAGC 1 cut(s) 1034
Bpu1102I GCTNAGC 2 cut(s) 609, 625
BpuEI CTTGAG 1 cut(s) 741
BsaAI YACGTR 1 cut(s) 1023
BsaHI GRCGYC 4 cut(s) 35, 71, 507, 872
BsaJI CCNNGG 6 cut(s) 379, 556, 675, 711, 854, 955
BsaWI WCCGGW 1 cut(s) 109
BsaXI ACNNNNNCTCC 6 cut(s) 206, 236, 407, 437, 1007, 1037
Bsc4I CCNNNNNNNGG 4 cut(s) 45, 283, 620, 992
Bse118I RCCGGY 2 cut(s) 169, 503
Bse3DI GCAATG 1 cut(s) 663
BseAI TCCGGA 1 cut(s) 109
BseBI CCWGG 2 cut(s) 542, 615
BseDI CCNNGG 6 cut(s) 379, 556, 675, 711, 854, 955
BseGI GGATG 1 cut(s) 727
BseLI CCNNNNNNNGG 4 cut(s) 45, 283, 620, 992
BseMI GCAATG 1 cut(s) 663
BseMII CTCAG 3 cut(s) 623, 639, 1025
BseX3I CGGCCG 3 cut(s) 167, 171, 708
BseXI GCAGC 5 cut(s) 517, 534, 617, 716, 862
Bsh1236I CGCG 5 cut(s) 334, 381, 536, 856, 909
Bsh1285I CGRYCG 5 cut(s) 170, 174, 281, 403, 711
BshFI GGCC 6 cut(s) 116, 169, 173, 498, 710, 724
BshNI GGYRCC 1 cut(s) 506
BsiEI CGRYCG 5 cut(s) 170, 174, 281, 403, 711
BsiHKAI GWGCWC 2 cut(s) 99, 1001
BsiHKCI CYCGRG 1 cut(s) 676
BslI CCNNNNNNNGG 4 cut(s) 45, 283, 620, 992
BsmAI GTCTC 1 cut(s) 912
BsmBI CGTCTC 1 cut(s) 912
BsnI GGCC 6 cut(s) 116, 169, 173, 498, 710, 724
BsoBI CYCGRG 1 cut(s) 676
Bsp1286I GDGCHC 2 cut(s) 99, 1001
Bsp13I TCCGGA 1 cut(s) 109
Bsp143I GATC 7 cut(s) 106, 146, 278, 352, 472, 832, 937
Bsp1720I GCTNAGC 2 cut(s) 609, 625
BspANI GGCC 6 cut(s) 116, 169, 173, 498, 710, 724
BspCNI CTCAG 3 cut(s) 622, 638, 1026
BspEI TCCGGA 1 cut(s) 109
BspFNI CGCG 5 cut(s) 334, 381, 536, 856, 909
BspHI TCATGA 1 cut(s) 940
BspLI GGNNCC 2 cut(s) 508, 984
BspMI ACCTGC 1 cut(s) 458
BspPI GGATC 4 cut(s) 101, 154, 840, 945
BspT107I GGYRCC 1 cut(s) 506
BsrBI CCGCTC 1 cut(s) 584
BsrDI GCAATG 1 cut(s) 663
BsrFI RCCGGY 2 cut(s) 169, 503
BssAI RCCGGY 2 cut(s) 169, 503
BssECI CCNNGG 6 cut(s) 379, 556, 675, 711, 854, 955
BssMI GATC 7 cut(s) 106, 146, 278, 352, 472, 832, 937
BssNI GRCGYC 4 cut(s) 35, 71, 507, 872
BssSI CACGAG 1 cut(s) 98
BssT1I CCWWGG 1 cut(s) 955
Bst2BI CACGAG 1 cut(s) 98
Bst2UI CCWGG 2 cut(s) 542, 615
Bst4CI ACNGT 3 cut(s) 404, 434, 521
Bst6I CTCTTC 1 cut(s) 810
BstACI GRCGYC 4 cut(s) 35, 71, 507, 872
BstBAI YACGTR 1 cut(s) 1023
BstC8I GCNNGC 4 cut(s) 171, 239, 505, 622
BstDEI CTNAG 3 cut(s) 609, 625, 1034
BstDSI CCRYGG 3 cut(s) 379, 556, 854
BstF5I GGATG 1 cut(s) 727
BstFNI CGCG 5 cut(s) 334, 381, 536, 856, 909
BstH2I RGCGCY 2 cut(s) 347, 510
BstHHI GCGC 3 cut(s) 346, 509, 909
BstKTI GATC 7 cut(s) 109, 149, 281, 355, 475, 835, 940
BstMAI GTCTC 1 cut(s) 912
BstMBI GATC 7 cut(s) 106, 146, 278, 352, 472, 832, 937
BstMCI CGRYCG 5 cut(s) 170, 174, 281, 403, 711
BstMWI GCNNNNNNNGC 9 cut(s) 179, 234, 369, 378, 390, 504, 626, 730, 856
BstNI CCWGG 2 cut(s) 542, 615
BstNSI RCATGY 1 cut(s) 605
BstPAI GACNNNNGTC 1 cut(s) 49
BstSCI CCNGG 2 cut(s) 540, 613
BstUI CGCG 5 cut(s) 334, 381, 536, 856, 909
BstV1I GCAGC 5 cut(s) 517, 534, 617, 716, 862
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BstZI CGGCCG 3 cut(s) 167, 171, 708
BsuRI GGCC 6 cut(s) 116, 169, 173, 498, 710, 724
BtgI CCRYGG 3 cut(s) 379, 556, 854
BtsCI GGATG 1 cut(s) 727
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 1 cut(s) 417
BveI ACCTGC 1 cut(s) 458
Cac8I GCNNGC 4 cut(s) 171, 239, 505, 622
CaiI CAGNNNCTG 1 cut(s) 547
CciI TCATGA 1 cut(s) 940
CfoI GCGC 3 cut(s) 346, 509, 909
Cfr10I RCCGGY 2 cut(s) 169, 503
Cfr13I GGNCC 1 cut(s) 16
Cfr42I CCGCGG 2 cut(s) 382, 857
CseI GACGC 4 cut(s) 24, 79, 880, 935
Csp6I GTAC 2 cut(s) 202, 802
CviAII CATG 2 cut(s) 602, 941
CviQI GTAC 2 cut(s) 202, 802
DdeI CTNAG 3 cut(s) 609, 625, 1034
DinI GGCGCC 1 cut(s) 508
DpnI GATC 7 cut(s) 108, 148, 280, 354, 474, 834, 939
DpnII GATC 7 cut(s) 106, 146, 278, 352, 472, 832, 937
EaeI YGGCCR 5 cut(s) 167, 171, 496, 708, 722
EagI CGGCCG 3 cut(s) 167, 171, 708
Eam1104I CTCTTC 1 cut(s) 810
EarI CTCTTC 1 cut(s) 810
EciI GGCGGA 8 cut(s) 57, 159, 224, 365, 576, 711, 720, 812
Ecl136II GAGCTC 2 cut(s) 97, 999
EclXI CGGCCG 3 cut(s) 167, 171, 708
Eco130I CCWWGG 1 cut(s) 955
Eco24I GRGCYC 2 cut(s) 99, 1001
Eco47I GGWCC 1 cut(s) 16
Eco52I CGGCCG 3 cut(s) 167, 171, 708
Eco53kI GAGCTC 2 cut(s) 97, 999
Eco88I CYCGRG 1 cut(s) 676
EcoICRI GAGCTC 2 cut(s) 97, 999
EcoRI GAATTC 1 cut(s) 176
EcoRII CCWGG 2 cut(s) 540, 613
EcoT14I CCWWGG 1 cut(s) 955
EcoT38I GRGCYC 2 cut(s) 99, 1001
EgeI GGCGCC 1 cut(s) 508
EheI GGCGCC 1 cut(s) 508
ErhI CCWWGG 1 cut(s) 955
Esp3I CGTCTC 1 cut(s) 912
FaeI CATG 2 cut(s) 605, 944
FaiI YATR 7 cut(s) 93, 158, 191, 252, 603, 650, 942
FatI CATG 2 cut(s) 601, 940
FauI CCCGC 1 cut(s) 802
FokI GGATG 1 cut(s) 714
FriOI GRGCYC 2 cut(s) 99, 1001
FseI GGCCGGCC 1 cut(s) 173
FspBI CTAG 2 cut(s) 570, 653
GlaI GCGC 3 cut(s) 345, 508, 908
GsuI CTGGAG 3 cut(s) 208, 409, 636
HaeII RGCGCY 2 cut(s) 347, 510
HaeIII GGCC 6 cut(s) 116, 169, 173, 498, 710, 724
HgaI GACGC 4 cut(s) 24, 79, 880, 935
HhaI GCGC 3 cut(s) 346, 509, 909
Hin1I GRCGYC 4 cut(s) 35, 71, 507, 872
Hin1II CATG 2 cut(s) 605, 944
Hin6I GCGC 3 cut(s) 344, 507, 907
HinP1I GCGC 3 cut(s) 344, 507, 907
HinfI GANTC 8 cut(s) 29, 50, 64, 325, 338, 512, 566, 865
HphI GGTGA 4 cut(s) 482, 757, 913, 985
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 6 cut(s) 69, 265, 517, 864, 870, 991
Hpy188III TCNNGA 7 cut(s) 110, 276, 308, 570, 758, 836, 941
Hpy8I GTNNAC 1 cut(s) 103
Hpy99I CGWCG 6 cut(s) 73, 122, 434, 465, 491, 521
HpyAV CCTTC 2 cut(s) 134, 260
HpyCH4III ACNGT 3 cut(s) 404, 434, 521
HpyCH4IV ACGT 2 cut(s) 89, 1022
HpyCH4V TGCA 2 cut(s) 550, 605
HpyF10VI GCNNNNNNNGC 9 cut(s) 179, 234, 369, 378, 390, 504, 626, 730, 856
HpyF3I CTNAG 3 cut(s) 609, 625, 1034
HpySE526I ACGT 2 cut(s) 89, 1022
Hsp92I GRCGYC 4 cut(s) 35, 71, 507, 872
Hsp92II CATG 2 cut(s) 605, 944
HspAI GCGC 3 cut(s) 344, 507, 907
KasI GGCGCC 1 cut(s) 506
Kpn2I TCCGGA 1 cut(s) 109
KroI GCCGGC 2 cut(s) 169, 503
KroNI GCCGGC 2 cut(s) 171, 505
KspI CCGCGG 2 cut(s) 382, 857
Kzo9I GATC 7 cut(s) 106, 146, 278, 352, 472, 832, 937
LmnI GCTCC 8 cut(s) 135, 544, 617, 701, 982, 996, 1004, 1030
Lsp1109I GCAGC 5 cut(s) 517, 534, 617, 716, 862
MaeI CTAG 2 cut(s) 570, 653
MaeII ACGT 2 cut(s) 89, 1022
MaeIII GTNAC 2 cut(s) 404, 641
MalI GATC 7 cut(s) 108, 148, 280, 354, 474, 834, 939
MbiI CCGCTC 1 cut(s) 584
MboI GATC 7 cut(s) 106, 146, 278, 352, 472, 832, 937
MboII GAAGA 2 cut(s) 362, 827
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 2 cut(s) 99, 1001
MluCI AATT 1 cut(s) 176
Mly113I GGCGCC 1 cut(s) 507
MlyI GAGTC 3 cut(s) 59, 319, 506
MmeI TCCRAC 3 cut(s) 92, 505, 540
MroI TCCGGA 1 cut(s) 109
MroNI GCCGGC 2 cut(s) 169, 503
MslI CAYNNNNRTG 1 cut(s) 251
MspA1I CMGCKG 3 cut(s) 381, 707, 856
MspR9I CCNGG 2 cut(s) 542, 615
MvaI CCWGG 2 cut(s) 542, 615
MvnI CGCG 5 cut(s) 334, 381, 536, 856, 909
MwoI GCNNNNNNNGC 9 cut(s) 179, 234, 369, 378, 390, 504, 626, 730, 856
NaeI GCCGGC 2 cut(s) 171, 505
NarI GGCGCC 1 cut(s) 507
NdeII GATC 7 cut(s) 106, 146, 278, 352, 472, 832, 937
NgoMIV GCCGGC 2 cut(s) 169, 503
NlaIII CATG 2 cut(s) 605, 944
NlaIV GGNNCC 2 cut(s) 508, 984
NmeAIII GCCGAG 2 cut(s) 736, 785
NspI RCATGY 1 cut(s) 605
OliI CACNNNNGTG 1 cut(s) 251
PagI TCATGA 1 cut(s) 940
PaqCI CACCTGC 1 cut(s) 458
PcsI WCGNNNNNNNCGW 2 cut(s) 59, 483
PdiI GCCGGC 2 cut(s) 171, 505
PfeI GAWTC 5 cut(s) 29, 64, 338, 566, 865
Ple19I CGATCG 1 cut(s) 281
PleI GAGTC 3 cut(s) 58, 319, 506
PluTI GGCGCC 1 cut(s) 510
PpsI GAGTC 3 cut(s) 58, 319, 506
Ppu21I YACGTR 1 cut(s) 1023
PshAI GACNNNNGTC 1 cut(s) 49
Psp124BI GAGCTC 2 cut(s) 99, 1001
Psp6I CCWGG 2 cut(s) 540, 613
PspGI CCWGG 2 cut(s) 540, 613
PspN4I GGNNCC 2 cut(s) 508, 984
PspPI GGNCC 1 cut(s) 16
PstNI CAGNNNCTG 1 cut(s) 547
PsuI RGATCY 1 cut(s) 352
PvuI CGATCG 1 cut(s) 281
RigI GGCCGGCC 1 cut(s) 173
RsaI GTAC 2 cut(s) 203, 803
RsaNI GTAC 2 cut(s) 202, 802
RseI CAYNNNNRTG 1 cut(s) 251
SacI GAGCTC 2 cut(s) 99, 1001
SacII CCGCGG 2 cut(s) 382, 857
Sau3AI GATC 7 cut(s) 106, 146, 278, 352, 472, 832, 937
Sau96I GGNCC 1 cut(s) 16
ScaI AGTACT 2 cut(s) 203, 803
SchI GAGTC 3 cut(s) 59, 319, 506
ScrFI CCNGG 2 cut(s) 542, 615
SduI GDGCHC 2 cut(s) 99, 1001
SfoI GGCGCC 1 cut(s) 508
Sfr303I CCGCGG 2 cut(s) 382, 857
SgrBI CCGCGG 2 cut(s) 382, 857
SinI GGWCC 1 cut(s) 16
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 1 cut(s) 756
SmoI CTYRAG 1 cut(s) 756
Sse9I AATT 1 cut(s) 176
SspDI GGCGCC 1 cut(s) 506
SspMI CTAG 2 cut(s) 570, 653
SstI GAGCTC 2 cut(s) 99, 1001
StyD4I CCNGG 2 cut(s) 540, 613
StyI CCWWGG 1 cut(s) 955
TaaI ACNGT 3 cut(s) 404, 434, 521
TaiI ACGT 2 cut(s) 92, 1025
TaqI TCGA 1 cut(s) 149
TasI AATT 1 cut(s) 176
TatI WGTACW 2 cut(s) 201, 801
TfiI GAWTC 5 cut(s) 29, 64, 338, 566, 865
TscAI CASTG 1 cut(s) 424
TseI GCWGC 6 cut(s) 5, 530, 547, 605, 704, 850
TspDTI ATGAA 1 cut(s) 145
TspRI CASTG 1 cut(s) 424
VpaK11BI GGWCC 1 cut(s) 16
XapI RAATTY 1 cut(s) 176
XbaI TCTAGA 1 cut(s) 569
XceI RCATGY 1 cut(s) 605
XspI CTAG 2 cut(s) 570, 653
ZrmI AGTACT 2 cut(s) 203, 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.