Rw7G015290

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0005627
Physical Location & Seq
Forward (+)
15392732 .. 15394968
2237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G015290.1

Sequence Viewer

Length: 1044 bp
ATGGCTGCGTTGCCGGACCAGAACGGCGATTCGGCGTCCGGCGGACGGGAGTCGCAGAGGACGATTCCGACGCCGTTTTTGACGAAAACGTATGAGCTCGTGGACGATCCGGAGGCCGACGATTTGATTTCGTGGAGCGAAGGCGGATCGACTTTCATAGTGTGGCGGCCGGCCGAATTTGCCAGAGATATACTCCCCAAGTACTTCAAACACAACAACTTCTCCAGCTTCGTCCGCCAGCTAAACACTTACGGATTTCGGAAGGTTGTTCCGGATCGGTGGGAGTTTGCCAACGACTGCTTCAAGAGAGGGGAGAAAGGACTCCTCCGCGAGATTCAGCGCCGGAAGATCTCTCCGGCGGTGTCCGCTTCCGCCGCGGCGGTTGTGGCGGCGGCGACCGTGACGGCGGCGACACTGCCTCCAGCGACGGCTTCGGTTTCTCCGGCAAACTCCGGCGACGAGCAGGTGATCTCGTCCAACTCGTCGCCGATGGCCGTGCCGGCGCCGACTCCGACGGTGGCGTTGCTGCCTCGAACCAGGAGCTGCAACACCACGGCGGAGATTCTAGAGGAGAACGAGCGGTTGAGGAAAGAGAACATGCAGCTCAGCCAGGAGCTGGCTCAGCTTCGGGGGTTGTGTAACAATATACTTGCAATGATGACCAACTACGCCTCGGGGCAGTTAGCGAGAGGCGGTGGAGCAGCGGCCGAGGATAAGCAGCCATATCCGCCGCCTCTTGCGGTGAAAAAATCTCAAGAGGGCGAAAACGCCGCCGTATCAGGCGGCGACAAGGCGGAGTACTCGGCGGGGGAAGAGGAGATGGAGGAGGATCAGGAGGAGGAAACGCCGCCGCTGCTTCCGATTCAGACGCCGAAGCTGTTTGGGGTTTCAATCGGGGTGAAGCGCGTGAGGAGAGACGAGGGGGAGGAGGAGGAGGATCATGAGCGTCAAAACCAAGGGTGTGATGAGGTGGTGAAGCCGGAGCCGTCGGATGGGAGCTCCAAGCACGATAAGGAGGCTACGTGGCTGGAGCTGAGGAAGTGA

Protein Analysis

347

Amino Acids

37.93

Weight (kDa)

4.89

Isoelectric Point (pI)

56.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 26 - 115 6e-31 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 454
Acc36I ACCTGC 1 cut(s) 454
AccB1I GGYRCC 1 cut(s) 502
AccB7I CCANNNNNTGG 1 cut(s) 616
AccBSI CCGCTC 1 cut(s) 580
AccII CGCG 3 cut(s) 330, 377, 906
AccIII TCCGGA 2 cut(s) 109, 271
AclWI GGATC 5 cut(s) 101, 154, 282, 837, 945
AcoI YGGCCR 4 cut(s) 167, 171, 492, 705
AcsI RAATTY 1 cut(s) 176
AcyI GRCGYC 4 cut(s) 35, 71, 503, 869
AfaI GTAC 2 cut(s) 203, 800
AfiI CCNNNNNNNGG 3 cut(s) 45, 616, 992
AgsI TTSAA 3 cut(s) 208, 304, 891
AjnI CCWGG 2 cut(s) 536, 609
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
Alw21I GWGCWC 2 cut(s) 99, 1001
Alw26I GTCTC 1 cut(s) 909
AlwI GGATC 5 cut(s) 101, 154, 282, 837, 945
AlwNI CAGNNNCTG 2 cut(s) 543, 616
Ama87I CYCGRG 1 cut(s) 673
Aor13HI TCCGGA 2 cut(s) 109, 271
AoxI GGCC 5 cut(s) 114, 167, 171, 492, 705
ApeKI GCWGC 7 cut(s) 5, 526, 543, 601, 701, 718, 853
ApoI RAATTY 1 cut(s) 176
ArsI GACNNNNNNTTYG 2 cut(s) 61, 93
AspLEI GCGC 3 cut(s) 342, 505, 906
AspS9I GGNCC 1 cut(s) 16
AsuHPI GGTGA 4 cut(s) 478, 754, 910, 985
AvaI CYCGRG 1 cut(s) 673
AvaII GGWCC 1 cut(s) 16
BanI GGYRCC 1 cut(s) 502
BanII GRGCYC 2 cut(s) 99, 1001
BauI CACGAG 1 cut(s) 98
Bbv12I GWGCWC 2 cut(s) 99, 1001
BbvCI CCTCAGC 1 cut(s) 1034
BbvI GCAGC 6 cut(s) 513, 530, 613, 713, 730, 840
BccI CCATC 3 cut(s) 484, 814, 986
BceAI ACGGC 8 cut(s) 40, 58, 420, 444, 479, 570, 758, 970
BcgI CGANNNNNNTGC 2 cut(s) 478, 512
BciT130I CCWGG 2 cut(s) 538, 611
BcoDI GTCTC 1 cut(s) 909
BfaI CTAG 1 cut(s) 566
BfoI RGCGCY 2 cut(s) 343, 506
BfuAI ACCTGC 1 cut(s) 454
BglI GCCNNNNNGGC 1 cut(s) 500
BglII AGATCT 1 cut(s) 348
BlpI GCTNAGC 2 cut(s) 605, 621
BmcAI AGTACT 2 cut(s) 203, 800
Bme1390I CCNGG 2 cut(s) 538, 611
Bme18I GGWCC 1 cut(s) 16
BmeT110I CYCGRG 1 cut(s) 673
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 2 cut(s) 504, 984
BmrFI CCNGG 2 cut(s) 538, 611
BoxI GACNNNNGTC 1 cut(s) 49
BplI GAGNNNNNCTC 2 cut(s) 177, 209
BpmI CTGGAG 2 cut(s) 208, 405
Bpu10I CCTNAGC 1 cut(s) 1034
Bpu1102I GCTNAGC 2 cut(s) 605, 621
BpuEI CTTGAG 1 cut(s) 738
BsaAI YACGTR 1 cut(s) 1023
BsaHI GRCGYC 4 cut(s) 35, 71, 503, 869
BsaJI CCNNGG 5 cut(s) 375, 552, 672, 708, 955
BsaWI WCCGGW 2 cut(s) 109, 271
BsaXI ACNNNNNCTCC 6 cut(s) 206, 236, 403, 433, 1007, 1037
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 616, 992
Bse118I RCCGGY 2 cut(s) 169, 499
Bse3DI GCAATG 1 cut(s) 660
BseAI TCCGGA 2 cut(s) 109, 271
BseBI CCWGG 2 cut(s) 538, 611
BseDI CCNNGG 5 cut(s) 375, 552, 672, 708, 955
BseGI GGATG 1 cut(s) 997
BseLI CCNNNNNNNGG 3 cut(s) 45, 616, 992
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 3 cut(s) 619, 635, 1025
BseRI GAGGAG 9 cut(s) 314, 584, 830, 839, 851, 925, 941, 944, 947
BseX3I CGGCCG 3 cut(s) 167, 171, 705
BseXI GCAGC 6 cut(s) 513, 530, 613, 713, 730, 840
Bsh1236I CGCG 3 cut(s) 330, 377, 906
Bsh1285I CGRYCG 4 cut(s) 170, 174, 399, 708
BshFI GGCC 5 cut(s) 116, 169, 173, 494, 707
BshNI GGYRCC 1 cut(s) 502
BsiEI CGRYCG 4 cut(s) 170, 174, 399, 708
BsiHKAI GWGCWC 2 cut(s) 99, 1001
BsiHKCI CYCGRG 1 cut(s) 673
BslI CCNNNNNNNGG 3 cut(s) 45, 616, 992
BsmAI GTCTC 1 cut(s) 909
BsmBI CGTCTC 1 cut(s) 909
BsnI GGCC 5 cut(s) 116, 169, 173, 494, 707
BsoBI CYCGRG 1 cut(s) 673
Bsp1286I GDGCHC 2 cut(s) 99, 1001
Bsp13I TCCGGA 2 cut(s) 109, 271
Bsp143I GATC 7 cut(s) 106, 146, 274, 348, 468, 829, 937
Bsp1720I GCTNAGC 2 cut(s) 605, 621
BspANI GGCC 5 cut(s) 116, 169, 173, 494, 707
BspCNI CTCAG 3 cut(s) 618, 634, 1026
BspEI TCCGGA 2 cut(s) 109, 271
BspFNI CGCG 3 cut(s) 330, 377, 906
BspHI TCATGA 1 cut(s) 940
BspLI GGNNCC 2 cut(s) 504, 984
BspMI ACCTGC 1 cut(s) 454
BspPI GGATC 5 cut(s) 101, 154, 282, 837, 945
BspT107I GGYRCC 1 cut(s) 502
BsrBI CCGCTC 1 cut(s) 580
BsrDI GCAATG 1 cut(s) 660
BsrFI RCCGGY 2 cut(s) 169, 499
BssAI RCCGGY 2 cut(s) 169, 499
BssECI CCNNGG 5 cut(s) 375, 552, 672, 708, 955
BssMI GATC 7 cut(s) 106, 146, 274, 348, 468, 829, 937
BssNI GRCGYC 4 cut(s) 35, 71, 503, 869
BssSI CACGAG 1 cut(s) 98
BssT1I CCWWGG 1 cut(s) 955
Bst2BI CACGAG 1 cut(s) 98
Bst2UI CCWGG 2 cut(s) 538, 611
Bst4CI ACNGT 2 cut(s) 400, 517
Bst6I CTCTTC 1 cut(s) 807
BstACI GRCGYC 4 cut(s) 35, 71, 503, 869
BstBAI YACGTR 1 cut(s) 1023
BstC8I GCNNGC 4 cut(s) 171, 239, 501, 618
BstDEI CTNAG 3 cut(s) 605, 621, 1034
BstDSI CCRYGG 2 cut(s) 375, 552
BstF5I GGATG 1 cut(s) 997
BstFNI CGCG 3 cut(s) 330, 377, 906
BstH2I RGCGCY 2 cut(s) 343, 506
BstHHI GCGC 3 cut(s) 342, 505, 906
BstKTI GATC 7 cut(s) 109, 149, 277, 351, 471, 832, 940
BstMAI GTCTC 1 cut(s) 909
BstMBI GATC 7 cut(s) 106, 146, 274, 348, 468, 829, 937
BstMCI CGRYCG 4 cut(s) 170, 174, 399, 708
BstMWI GCNNNNNNNGC 9 cut(s) 179, 234, 365, 374, 386, 500, 622, 727, 853
BstNI CCWGG 2 cut(s) 538, 611
BstNSI RCATGY 1 cut(s) 601
BstPAI GACNNNNGTC 1 cut(s) 49
BstSCI CCNGG 2 cut(s) 536, 609
BstUI CGCG 3 cut(s) 330, 377, 906
BstV1I GCAGC 6 cut(s) 513, 530, 613, 713, 730, 840
BstX2I RGATCY 1 cut(s) 348
BstYI RGATCY 1 cut(s) 348
BstZI CGGCCG 3 cut(s) 167, 171, 705
BsuRI GGCC 5 cut(s) 116, 169, 173, 494, 707
BtgI CCRYGG 2 cut(s) 375, 552
BtsCI GGATG 1 cut(s) 997
BtsI GCAGTG 1 cut(s) 413
BtsIMutI CAGTG 1 cut(s) 413
BveI ACCTGC 1 cut(s) 454
Cac8I GCNNGC 4 cut(s) 171, 239, 501, 618
CaiI CAGNNNCTG 2 cut(s) 543, 616
CciI TCATGA 1 cut(s) 940
CfoI GCGC 3 cut(s) 342, 505, 906
Cfr10I RCCGGY 2 cut(s) 169, 499
Cfr13I GGNCC 1 cut(s) 16
Cfr42I CCGCGG 1 cut(s) 378
CseI GACGC 4 cut(s) 24, 79, 877, 935
Csp6I GTAC 2 cut(s) 202, 799
CviAII CATG 2 cut(s) 598, 941
CviQI GTAC 2 cut(s) 202, 799
DdeI CTNAG 3 cut(s) 605, 621, 1034
DinI GGCGCC 1 cut(s) 504
DpnI GATC 7 cut(s) 108, 148, 276, 350, 470, 831, 939
DpnII GATC 7 cut(s) 106, 146, 274, 348, 468, 829, 937
EaeI YGGCCR 4 cut(s) 167, 171, 492, 705
EagI CGGCCG 3 cut(s) 167, 171, 705
Eam1104I CTCTTC 1 cut(s) 807
EarI CTCTTC 1 cut(s) 807
EciI GGCGGA 7 cut(s) 57, 159, 224, 361, 572, 717, 809
Ecl136II GAGCTC 2 cut(s) 97, 999
EclXI CGGCCG 3 cut(s) 167, 171, 705
Eco130I CCWWGG 1 cut(s) 955
Eco24I GRGCYC 2 cut(s) 99, 1001
Eco47I GGWCC 1 cut(s) 16
Eco52I CGGCCG 3 cut(s) 167, 171, 705
Eco53kI GAGCTC 2 cut(s) 97, 999
Eco88I CYCGRG 1 cut(s) 673
EcoICRI GAGCTC 2 cut(s) 97, 999
EcoRII CCWGG 2 cut(s) 536, 609
EcoT14I CCWWGG 1 cut(s) 955
EcoT38I GRGCYC 2 cut(s) 99, 1001
EgeI GGCGCC 1 cut(s) 504
EheI GGCGCC 1 cut(s) 504
ErhI CCWWGG 1 cut(s) 955
Esp3I CGTCTC 1 cut(s) 909
FaeI CATG 2 cut(s) 601, 944
FaiI YATR 7 cut(s) 93, 158, 191, 599, 647, 724, 942
FatI CATG 2 cut(s) 597, 940
FauI CCCGC 1 cut(s) 799
FokI GGATG 1 cut(s) 1004
FriOI GRGCYC 2 cut(s) 99, 1001
FseI GGCCGGCC 1 cut(s) 173
FspBI CTAG 1 cut(s) 566
GlaI GCGC 3 cut(s) 341, 504, 905
GsuI CTGGAG 2 cut(s) 208, 405
HaeII RGCGCY 2 cut(s) 343, 506
HaeIII GGCC 5 cut(s) 116, 169, 173, 494, 707
HgaI GACGC 4 cut(s) 24, 79, 877, 935
HhaI GCGC 3 cut(s) 342, 505, 906
Hin1I GRCGYC 4 cut(s) 35, 71, 503, 869
Hin1II CATG 2 cut(s) 601, 944
Hin6I GCGC 3 cut(s) 340, 503, 904
HinP1I GCGC 3 cut(s) 340, 503, 904
HinfI GANTC 8 cut(s) 29, 50, 64, 321, 334, 508, 562, 862
HphI GGTGA 4 cut(s) 478, 754, 910, 985
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 6 cut(s) 69, 261, 513, 861, 867, 991
Hpy188III TCNNGA 7 cut(s) 110, 272, 304, 566, 755, 833, 941
Hpy8I GTNNAC 1 cut(s) 103
Hpy99I CGWCG 7 cut(s) 73, 122, 430, 461, 487, 517, 991
HpyAV CCTTC 2 cut(s) 134, 256
HpyCH4III ACNGT 2 cut(s) 400, 517
HpyCH4IV ACGT 2 cut(s) 89, 1022
HpyCH4V TGCA 3 cut(s) 546, 601, 653
HpyF10VI GCNNNNNNNGC 9 cut(s) 179, 234, 365, 374, 386, 500, 622, 727, 853
HpyF3I CTNAG 3 cut(s) 605, 621, 1034
HpySE526I ACGT 2 cut(s) 89, 1022
Hsp92I GRCGYC 4 cut(s) 35, 71, 503, 869
Hsp92II CATG 2 cut(s) 601, 944
HspAI GCGC 3 cut(s) 340, 503, 904
KasI GGCGCC 1 cut(s) 502
Kpn2I TCCGGA 2 cut(s) 109, 271
KroI GCCGGC 2 cut(s) 169, 499
KroNI GCCGGC 2 cut(s) 171, 501
KspI CCGCGG 1 cut(s) 378
Kzo9I GATC 7 cut(s) 106, 146, 274, 348, 468, 829, 937
LmnI GCTCC 8 cut(s) 135, 540, 613, 698, 982, 996, 1004, 1030
Lsp1109I GCAGC 6 cut(s) 513, 530, 613, 713, 730, 840
MaeI CTAG 1 cut(s) 566
MaeII ACGT 2 cut(s) 89, 1022
MaeIII GTNAC 2 cut(s) 400, 638
MalI GATC 7 cut(s) 108, 148, 276, 350, 470, 831, 939
MbiI CCGCTC 1 cut(s) 580
MboI GATC 7 cut(s) 106, 146, 274, 348, 468, 829, 937
MboII GAAGA 2 cut(s) 358, 824
MflI RGATCY 1 cut(s) 348
MhlI GDGCHC 2 cut(s) 99, 1001
MluCI AATT 1 cut(s) 176
Mly113I GGCGCC 1 cut(s) 503
MlyI GAGTC 3 cut(s) 59, 315, 502
MmeI TCCRAC 4 cut(s) 92, 501, 536, 969
MroI TCCGGA 2 cut(s) 109, 271
MroNI GCCGGC 2 cut(s) 169, 499
MspA1I CMGCKG 3 cut(s) 377, 704, 853
MspR9I CCNGG 2 cut(s) 538, 611
MvaI CCWGG 2 cut(s) 538, 611
MvnI CGCG 3 cut(s) 330, 377, 906
MwoI GCNNNNNNNGC 9 cut(s) 179, 234, 365, 374, 386, 500, 622, 727, 853
NaeI GCCGGC 2 cut(s) 171, 501
NarI GGCGCC 1 cut(s) 503
NdeII GATC 7 cut(s) 106, 146, 274, 348, 468, 829, 937
NgoMIV GCCGGC 2 cut(s) 169, 499
NlaIII CATG 2 cut(s) 601, 944
NlaIV GGNNCC 2 cut(s) 504, 984
NmeAIII GCCGAG 2 cut(s) 733, 782
NmuCI GTSAC 1 cut(s) 400
NspI RCATGY 1 cut(s) 601
PagI TCATGA 1 cut(s) 940
PaqCI CACCTGC 1 cut(s) 454
PcsI WCGNNNNNNNCGW 2 cut(s) 59, 479
PdiI GCCGGC 2 cut(s) 171, 501
PfeI GAWTC 5 cut(s) 29, 64, 334, 562, 862
PflMI CCANNNNNTGG 1 cut(s) 616
PleI GAGTC 3 cut(s) 58, 315, 502
PluTI GGCGCC 1 cut(s) 506
PpsI GAGTC 3 cut(s) 58, 315, 502
Ppu21I YACGTR 1 cut(s) 1023
PshAI GACNNNNGTC 1 cut(s) 49
Psp124BI GAGCTC 2 cut(s) 99, 1001
Psp6I CCWGG 2 cut(s) 536, 609
PspGI CCWGG 2 cut(s) 536, 609
PspN4I GGNNCC 2 cut(s) 504, 984
PspPI GGNCC 1 cut(s) 16
PstNI CAGNNNCTG 2 cut(s) 543, 616
PsuI RGATCY 1 cut(s) 348
RigI GGCCGGCC 1 cut(s) 173
RsaI GTAC 2 cut(s) 203, 800
RsaNI GTAC 2 cut(s) 202, 799
SacI GAGCTC 2 cut(s) 99, 1001
SacII CCGCGG 1 cut(s) 378
Sau3AI GATC 7 cut(s) 106, 146, 274, 348, 468, 829, 937
Sau96I GGNCC 1 cut(s) 16
ScaI AGTACT 2 cut(s) 203, 800
SchI GAGTC 3 cut(s) 59, 315, 502
ScrFI CCNGG 2 cut(s) 538, 611
SduI GDGCHC 2 cut(s) 99, 1001
SfoI GGCGCC 1 cut(s) 504
Sfr303I CCGCGG 1 cut(s) 378
SgrBI CCGCGG 1 cut(s) 378
SinI GGWCC 1 cut(s) 16
SmlI CTYRAG 1 cut(s) 753
SmoI CTYRAG 1 cut(s) 753
Sse9I AATT 1 cut(s) 176
SspDI GGCGCC 1 cut(s) 502
SspMI CTAG 1 cut(s) 566
SstI GAGCTC 2 cut(s) 99, 1001
StyD4I CCNGG 2 cut(s) 536, 609
StyI CCWWGG 1 cut(s) 955
TaaI ACNGT 2 cut(s) 400, 517
TaiI ACGT 2 cut(s) 92, 1025
TaqI TCGA 2 cut(s) 149, 532
TasI AATT 1 cut(s) 176
TatI WGTACW 2 cut(s) 201, 798
TfiI GAWTC 5 cut(s) 29, 64, 334, 562, 862
TscAI CASTG 1 cut(s) 420
TseFI GTSAC 1 cut(s) 400
TseI GCWGC 7 cut(s) 5, 526, 543, 601, 701, 718, 853
Tsp45I GTSAC 1 cut(s) 400
TspDTI ATGAA 1 cut(s) 145
TspGWI ACGGA 1 cut(s) 267
TspRI CASTG 1 cut(s) 420
Van91I CCANNNNNTGG 1 cut(s) 616
VpaK11BI GGWCC 1 cut(s) 16
XapI RAATTY 1 cut(s) 176
XbaI TCTAGA 1 cut(s) 565
XceI RCATGY 1 cut(s) 601
XspI CTAG 1 cut(s) 566
ZrmI AGTACT 2 cut(s) 203, 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.