RLG00000003831

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0005627
Physical Location & Seq
Reverse (-)
53259577 .. 53262090
2514 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003831

Sequence Viewer

Length: 1155 bp
ATGAGCTTTGGACTGTTAACCCAACACTTCTGTAATAGGGCCAAGGATTTGAATTATTTCTTCGCGAAAGTTCTCTCTGGATCTGAGAGAGGGGAGAGGCAAAGGGCCATGGCTGCGTTGCCGGACCAGAACGGCGATTCGGCGTCCGGCGGCGGCGGACGGGAGTCTCAAAGGACGATTCCGACGCCGTTTTTGACGAAAACGTATGAGCTCGTGGACGATCCGGAGGCCGACGATTTGATTTCGTGGAGCGAAGGCGGATCGACTTTCATAGTGTGGCGGCCGGCCGAATTTGCCAGAGATATACTCCCCAAGTACTTCAAACACAACAACTTCTCCAGCTTCGTCCGCCAGCTAAACACTTATGGATTTCGGAAGGTTGTTCCGGATCGGTGGGAGTTTGCCAACGACTGCTTCAAAAGAGGGGAGAAAGGACTCCTCCGCGAGATTCAGCGCCGGAAGATCTCTCCGGCGGTGTCCGCTTCCGCCGCTGCGGTTGTGGCGGCGGCGACCGTGACGGCGGCGACACTGCCTCCAGCGACAGTTTCGGTTTCTCCGTCAAACTCCGGCGACGAGCAGGTGATCTCGTCCAACTCGTCGCCGATGACCGTGCCGGCGCCGACTCCGACGGTGGTATTGCTGCCGCGAACAAGGAGCTGCAACACCACGGCGGAGATTCTAGAGGAGAACGAGCGGTTGAGGAAAGAGAACATGCAGCTCAGCCAGGAGCTGGCTCAGCTTCGGGGGTTGTGTAACAATATACTAGCAATGATGACCAACTACGCCTCGGGGCAGTTAGCGAGAGGCGGCGGAGCAGCGGCCGAGGATAAGCGGCCACATCCGCCGCCTCTTGCGGTGAAAAAATCTCAAGAGGGGGAAAACGCCGCCGTATCAGGCGGCGACAAGGCGGAGTACTCGGCGGGGGAAGAGGAGATGGAAGAGGATCAGGAGGAGGAAACGCCGCCGCCGATTCCGATTCAGACGCCGAAGCTGTTTGGGGTTTCAATCGGGGTGAAGCGCGTGAGGAGAGACGAGGAGGAGGGGGAGGATCATGAGCGTCAAAACCAAGGGTGCGATGAGGTGGTGAAGCCGGAGCCGTCGGATGGGAGCTCCAAGCACGATAAGGATGCTACGTGGCTGGAGCTGAGGAAGTGA

Protein Analysis

385

Amino Acids

42.15

Weight (kDa)

5.28

Isoelectric Point (pI)

52.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 64 - 153 7.2e-31 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 568
Acc36I ACCTGC 1 cut(s) 568
AccB1I GGYRCC 1 cut(s) 616
AccB7I CCANNNNNTGG 1 cut(s) 730
AccBSI CCGCTC 1 cut(s) 694
AccII CGCG 4 cut(s) 65, 444, 646, 1020
AccIII TCCGGA 2 cut(s) 223, 385
AclWI GGATC 6 cut(s) 88, 215, 268, 396, 951, 1056
AcoI YGGCCR 4 cut(s) 281, 285, 819, 833
AcsI RAATTY 1 cut(s) 290
AcyI GRCGYC 4 cut(s) 143, 185, 617, 983
AfaI GTAC 2 cut(s) 317, 914
AfiI CCNNNNNNNGG 2 cut(s) 730, 1103
AgsI TTSAA 4 cut(s) 52, 322, 418, 1005
AjnI CCWGG 1 cut(s) 723
AjuI GAANNNNNNNTTGG 2 cut(s) 398, 430
Alw21I GWGCWC 2 cut(s) 213, 1112
Alw26I GTCTC 2 cut(s) 171, 1023
AlwI GGATC 6 cut(s) 88, 215, 268, 396, 951, 1056
AlwNI CAGNNNCTG 1 cut(s) 730
Ama87I CYCGRG 1 cut(s) 787
Aor13HI TCCGGA 2 cut(s) 223, 385
AoxI GGCC 7 cut(s) 39, 105, 228, 281, 285, 819, 833
ApeKI GCWGC 6 cut(s) 113, 491, 640, 657, 715, 815
ApoI RAATTY 1 cut(s) 290
ArsI GACNNNNNNTTYG 2 cut(s) 175, 207
Asp700I GAANNNNTTC 1 cut(s) 56
AspLEI GCGC 3 cut(s) 456, 619, 1020
AspS9I GGNCC 3 cut(s) 39, 105, 124
AsuHPI GGTGA 4 cut(s) 592, 868, 1024, 1096
AvaI CYCGRG 1 cut(s) 787
AvaII GGWCC 1 cut(s) 124
BanI GGYRCC 1 cut(s) 616
BanII GRGCYC 2 cut(s) 213, 1112
BauI CACGAG 1 cut(s) 212
Bbv12I GWGCWC 2 cut(s) 213, 1112
BbvCI CCTCAGC 1 cut(s) 1145
BbvI GCAGC 6 cut(s) 100, 478, 627, 644, 727, 827
BccI CCATC 2 cut(s) 928, 1097
BceAI ACGGC 6 cut(s) 148, 172, 534, 684, 872, 1081
BcgI CGANNNNNNTGC 4 cut(s) 592, 626, 1109, 1143
BciT130I CCWGG 1 cut(s) 725
BcoDI GTCTC 2 cut(s) 171, 1023
BfaI CTAG 2 cut(s) 680, 764
BfoI RGCGCY 2 cut(s) 457, 620
BfuAI ACCTGC 1 cut(s) 568
BglII AGATCT 1 cut(s) 462
BlpI GCTNAGC 2 cut(s) 719, 735
BmcAI AGTACT 2 cut(s) 317, 914
Bme1390I CCNGG 1 cut(s) 725
Bme18I GGWCC 1 cut(s) 124
BmeT110I CYCGRG 1 cut(s) 787
BmgT120I GGNCC 3 cut(s) 39, 105, 124
BmiI GGNNCC 2 cut(s) 618, 1095
BmrFI CCNGG 1 cut(s) 725
BmsI GCATC 1 cut(s) 1117
BoxI GACNNNNGTC 1 cut(s) 163
BplI GAGNNNNNCTC 2 cut(s) 291, 323
BpmI CTGGAG 2 cut(s) 322, 519
Bpu10I CCTNAGC 1 cut(s) 1145
Bpu1102I GCTNAGC 2 cut(s) 719, 735
BpuEI CTTGAG 1 cut(s) 852
BsaAI YACGTR 1 cut(s) 1134
BsaHI GRCGYC 4 cut(s) 143, 185, 617, 983
BsaJI CCNNGG 6 cut(s) 42, 108, 666, 786, 822, 1066
BsaWI WCCGGW 2 cut(s) 223, 385
BsaXI ACNNNNNCTCC 4 cut(s) 320, 350, 517, 547
Bsc4I CCNNNNNNNGG 2 cut(s) 730, 1103
Bse118I RCCGGY 2 cut(s) 283, 613
Bse3DI GCAATG 1 cut(s) 774
BseAI TCCGGA 2 cut(s) 223, 385
BseBI CCWGG 1 cut(s) 725
BseDI CCNNGG 6 cut(s) 42, 108, 666, 786, 822, 1066
BseGI GGATG 3 cut(s) 838, 1108, 1132
BseLI CCNNNNNNNGG 2 cut(s) 730, 1103
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 4 cut(s) 75, 733, 749, 1136
BseRI GAGGAG 7 cut(s) 428, 698, 944, 965, 1039, 1049, 1052
BseX3I CGGCCG 3 cut(s) 281, 285, 819
BseXI GCAGC 6 cut(s) 100, 478, 627, 644, 727, 827
Bsh1236I CGCG 4 cut(s) 65, 444, 646, 1020
Bsh1285I CGRYCG 4 cut(s) 284, 288, 513, 822
BshFI GGCC 7 cut(s) 41, 107, 230, 283, 287, 821, 835
BshNI GGYRCC 1 cut(s) 616
BsiEI CGRYCG 4 cut(s) 284, 288, 513, 822
BsiHKAI GWGCWC 2 cut(s) 213, 1112
BsiHKCI CYCGRG 1 cut(s) 787
BslI CCNNNNNNNGG 2 cut(s) 730, 1103
BsmAI GTCTC 2 cut(s) 171, 1023
BsmBI CGTCTC 1 cut(s) 1023
BsnI GGCC 7 cut(s) 41, 107, 230, 283, 287, 821, 835
BsoBI CYCGRG 1 cut(s) 787
Bsp1286I GDGCHC 2 cut(s) 213, 1112
Bsp13I TCCGGA 2 cut(s) 223, 385
Bsp143I GATC 8 cut(s) 80, 220, 260, 388, 462, 582, 943, 1048
Bsp1720I GCTNAGC 2 cut(s) 719, 735
Bsp19I CCATGG 1 cut(s) 108
Bsp68I TCGCGA 1 cut(s) 65
BspANI GGCC 7 cut(s) 41, 107, 230, 283, 287, 821, 835
BspCNI CTCAG 4 cut(s) 76, 732, 748, 1137
BspEI TCCGGA 2 cut(s) 223, 385
BspFNI CGCG 4 cut(s) 65, 444, 646, 1020
BspHI TCATGA 1 cut(s) 1051
BspLI GGNNCC 2 cut(s) 618, 1095
BspMI ACCTGC 1 cut(s) 568
BspPI GGATC 6 cut(s) 88, 215, 268, 396, 951, 1056
BspT107I GGYRCC 1 cut(s) 616
BsrBI CCGCTC 1 cut(s) 694
BsrDI GCAATG 1 cut(s) 774
BsrFI RCCGGY 2 cut(s) 283, 613
BssAI RCCGGY 2 cut(s) 283, 613
BssECI CCNNGG 6 cut(s) 42, 108, 666, 786, 822, 1066
BssMI GATC 8 cut(s) 80, 220, 260, 388, 462, 582, 943, 1048
BssNI GRCGYC 4 cut(s) 143, 185, 617, 983
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 3 cut(s) 42, 108, 1066
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 1 cut(s) 725
Bst4CI ACNGT 5 cut(s) 15, 514, 544, 610, 631
Bst6I CTCTTC 2 cut(s) 921, 933
BstACI GRCGYC 4 cut(s) 143, 185, 617, 983
BstBAI YACGTR 1 cut(s) 1134
BstC8I GCNNGC 4 cut(s) 285, 353, 615, 732
BstDEI CTNAG 4 cut(s) 84, 719, 735, 1145
BstDSI CCRYGG 2 cut(s) 108, 666
BstF5I GGATG 3 cut(s) 838, 1108, 1132
BstFNI CGCG 4 cut(s) 65, 444, 646, 1020
BstH2I RGCGCY 2 cut(s) 457, 620
BstHHI GCGC 3 cut(s) 456, 619, 1020
BstKTI GATC 8 cut(s) 83, 223, 263, 391, 465, 585, 946, 1051
BstMAI GTCTC 2 cut(s) 171, 1023
BstMBI GATC 8 cut(s) 80, 220, 260, 388, 462, 582, 943, 1048
BstMCI CGRYCG 4 cut(s) 284, 288, 513, 822
BstMWI GCNNNNNNNGC 8 cut(s) 113, 293, 348, 479, 488, 500, 736, 841
BstNI CCWGG 1 cut(s) 725
BstNSI RCATGY 1 cut(s) 715
BstPAI GACNNNNGTC 1 cut(s) 163
BstSCI CCNGG 1 cut(s) 723
BstUI CGCG 4 cut(s) 65, 444, 646, 1020
BstV1I GCAGC 6 cut(s) 100, 478, 627, 644, 727, 827
BstX2I RGATCY 2 cut(s) 80, 462
BstYI RGATCY 2 cut(s) 80, 462
BstZI CGGCCG 3 cut(s) 281, 285, 819
BsuRI GGCC 7 cut(s) 41, 107, 230, 283, 287, 821, 835
BtgI CCRYGG 2 cut(s) 108, 666
BtgZI GCGATG 1 cut(s) 1089
BtsCI GGATG 3 cut(s) 838, 1108, 1132
BtsI GCAGTG 1 cut(s) 527
BtsIMutI CAGTG 1 cut(s) 527
BtuMI TCGCGA 1 cut(s) 65
BveI ACCTGC 1 cut(s) 568
Cac8I GCNNGC 4 cut(s) 285, 353, 615, 732
CaiI CAGNNNCTG 1 cut(s) 730
CciI TCATGA 1 cut(s) 1051
CfoI GCGC 3 cut(s) 456, 619, 1020
Cfr10I RCCGGY 2 cut(s) 283, 613
Cfr13I GGNCC 3 cut(s) 39, 105, 124
CseI GACGC 4 cut(s) 132, 193, 991, 1046
Csp6I GTAC 2 cut(s) 316, 913
CviAII CATG 3 cut(s) 109, 712, 1052
CviQI GTAC 2 cut(s) 316, 913
DdeI CTNAG 4 cut(s) 84, 719, 735, 1145
DinI GGCGCC 1 cut(s) 618
DpnI GATC 8 cut(s) 82, 222, 262, 390, 464, 584, 945, 1050
DpnII GATC 8 cut(s) 80, 220, 260, 388, 462, 582, 943, 1048
EaeI YGGCCR 4 cut(s) 281, 285, 819, 833
EagI CGGCCG 3 cut(s) 281, 285, 819
Eam1104I CTCTTC 2 cut(s) 921, 933
EarI CTCTTC 2 cut(s) 921, 933
EciI GGCGGA 8 cut(s) 171, 273, 338, 475, 686, 825, 831, 923
Ecl136II GAGCTC 2 cut(s) 211, 1110
EclXI CGGCCG 3 cut(s) 281, 285, 819
Eco130I CCWWGG 3 cut(s) 42, 108, 1066
Eco24I GRGCYC 2 cut(s) 213, 1112
Eco47I GGWCC 1 cut(s) 124
Eco52I CGGCCG 3 cut(s) 281, 285, 819
Eco53kI GAGCTC 2 cut(s) 211, 1110
Eco88I CYCGRG 1 cut(s) 787
EcoICRI GAGCTC 2 cut(s) 211, 1110
EcoRII CCWGG 1 cut(s) 723
EcoT14I CCWWGG 3 cut(s) 42, 108, 1066
EcoT38I GRGCYC 2 cut(s) 213, 1112
EgeI GGCGCC 1 cut(s) 618
EheI GGCGCC 1 cut(s) 618
ErhI CCWWGG 3 cut(s) 42, 108, 1066
Esp3I CGTCTC 1 cut(s) 1023
FaeI CATG 3 cut(s) 112, 715, 1055
FaiI YATR 8 cut(s) 110, 207, 272, 305, 366, 713, 761, 1053
FatI CATG 3 cut(s) 108, 711, 1051
FauI CCCGC 1 cut(s) 913
FokI GGATG 3 cut(s) 825, 1115, 1139
FriOI GRGCYC 2 cut(s) 213, 1112
FseI GGCCGGCC 1 cut(s) 287
FspBI CTAG 2 cut(s) 680, 764
GlaI GCGC 3 cut(s) 455, 618, 1019
GsuI CTGGAG 2 cut(s) 322, 519
HaeII RGCGCY 2 cut(s) 457, 620
HaeIII GGCC 7 cut(s) 41, 107, 230, 283, 287, 821, 835
HgaI GACGC 4 cut(s) 132, 193, 991, 1046
HhaI GCGC 3 cut(s) 456, 619, 1020
Hin1I GRCGYC 4 cut(s) 143, 185, 617, 983
Hin1II CATG 3 cut(s) 112, 715, 1055
Hin6I GCGC 3 cut(s) 454, 617, 1018
HinP1I GCGC 3 cut(s) 454, 617, 1018
HincII GTYRAC 1 cut(s) 18
HindII GTYRAC 1 cut(s) 18
HinfI GANTC 9 cut(s) 137, 164, 178, 435, 448, 622, 676, 970, 976
HpaI GTTAAC 1 cut(s) 18
HphI GGTGA 4 cut(s) 592, 868, 1024, 1096
Hpy166II GTNNAC 2 cut(s) 18, 217
Hpy188I TCNGA 7 cut(s) 85, 183, 375, 627, 975, 981, 1102
Hpy188III TCNNGA 8 cut(s) 64, 78, 224, 386, 680, 869, 947, 1052
Hpy8I GTNNAC 2 cut(s) 18, 217
Hpy99I CGWCG 6 cut(s) 187, 236, 575, 601, 631, 1102
HpyAV CCTTC 2 cut(s) 248, 370
HpyCH4III ACNGT 5 cut(s) 15, 514, 544, 610, 631
HpyCH4IV ACGT 2 cut(s) 203, 1133
HpyCH4V TGCA 2 cut(s) 660, 715
HpyF10VI GCNNNNNNNGC 8 cut(s) 113, 293, 348, 479, 488, 500, 736, 841
HpyF3I CTNAG 4 cut(s) 84, 719, 735, 1145
HpySE526I ACGT 2 cut(s) 203, 1133
Hsp92I GRCGYC 4 cut(s) 143, 185, 617, 983
Hsp92II CATG 3 cut(s) 112, 715, 1055
HspAI GCGC 3 cut(s) 454, 617, 1018
KasI GGCGCC 1 cut(s) 616
Kpn2I TCCGGA 2 cut(s) 223, 385
KroI GCCGGC 2 cut(s) 283, 613
KroNI GCCGGC 2 cut(s) 285, 615
KspAI GTTAAC 1 cut(s) 18
Kzo9I GATC 8 cut(s) 80, 220, 260, 388, 462, 582, 943, 1048
LmnI GCTCC 8 cut(s) 249, 654, 727, 812, 1093, 1107, 1115, 1141
Lsp1109I GCAGC 6 cut(s) 100, 478, 627, 644, 727, 827
LweI GCATC 1 cut(s) 1117
MaeI CTAG 2 cut(s) 680, 764
MaeII ACGT 2 cut(s) 203, 1133
MaeIII GTNAC 2 cut(s) 514, 752
MalI GATC 8 cut(s) 82, 222, 262, 390, 464, 584, 945, 1050
MbiI CCGCTC 1 cut(s) 694
MboI GATC 8 cut(s) 80, 220, 260, 388, 462, 582, 943, 1048
MboII GAAGA 4 cut(s) 52, 472, 938, 950
MflI RGATCY 2 cut(s) 80, 462
MhlI GDGCHC 2 cut(s) 213, 1112
MluCI AATT 2 cut(s) 52, 290
Mly113I GGCGCC 1 cut(s) 617
MlyI GAGTC 3 cut(s) 173, 429, 616
MmeI TCCRAC 4 cut(s) 206, 615, 650, 1080
MroI TCCGGA 2 cut(s) 223, 385
MroNI GCCGGC 2 cut(s) 283, 613
MroXI GAANNNNTTC 1 cut(s) 56
MseI TTAA 1 cut(s) 17
MspA1I CMGCKG 2 cut(s) 491, 818
MspR9I CCNGG 1 cut(s) 725
MvaI CCWGG 1 cut(s) 725
MvnI CGCG 4 cut(s) 65, 444, 646, 1020
MwoI GCNNNNNNNGC 8 cut(s) 113, 293, 348, 479, 488, 500, 736, 841
NaeI GCCGGC 2 cut(s) 285, 615
NarI GGCGCC 1 cut(s) 617
NcoI CCATGG 1 cut(s) 108
NdeII GATC 8 cut(s) 80, 220, 260, 388, 462, 582, 943, 1048
NgoMIV GCCGGC 2 cut(s) 283, 613
NlaIII CATG 3 cut(s) 112, 715, 1055
NlaIV GGNNCC 2 cut(s) 618, 1095
NmeAIII GCCGAG 2 cut(s) 847, 896
NmuCI GTSAC 1 cut(s) 514
NruI TCGCGA 1 cut(s) 65
NspI RCATGY 1 cut(s) 715
PagI TCATGA 1 cut(s) 1051
PaqCI CACCTGC 1 cut(s) 568
PcsI WCGNNNNNNNCGW 3 cut(s) 554, 593, 965
PdiI GCCGGC 2 cut(s) 285, 615
PdmI GAANNNNTTC 1 cut(s) 56
PfeI GAWTC 6 cut(s) 137, 178, 448, 676, 970, 976
PflMI CCANNNNNTGG 1 cut(s) 730
PleI GAGTC 3 cut(s) 172, 429, 616
PluTI GGCGCC 1 cut(s) 620
PpsI GAGTC 3 cut(s) 172, 429, 616
Ppu21I YACGTR 1 cut(s) 1134
PshAI GACNNNNGTC 1 cut(s) 163
Psp124BI GAGCTC 2 cut(s) 213, 1112
Psp6I CCWGG 1 cut(s) 723
PspGI CCWGG 1 cut(s) 723
PspN4I GGNNCC 2 cut(s) 618, 1095
PspPI GGNCC 3 cut(s) 39, 105, 124
PstNI CAGNNNCTG 1 cut(s) 730
PsuI RGATCY 2 cut(s) 80, 462
RigI GGCCGGCC 1 cut(s) 287
RruI TCGCGA 1 cut(s) 65
RsaI GTAC 2 cut(s) 317, 914
RsaNI GTAC 2 cut(s) 316, 913
SacI GAGCTC 2 cut(s) 213, 1112
SaqAI TTAA 1 cut(s) 17
Sau3AI GATC 8 cut(s) 80, 220, 260, 388, 462, 582, 943, 1048
Sau96I GGNCC 3 cut(s) 39, 105, 124
ScaI AGTACT 2 cut(s) 317, 914
SchI GAGTC 3 cut(s) 173, 429, 616
ScrFI CCNGG 1 cut(s) 725
SduI GDGCHC 2 cut(s) 213, 1112
SfaNI GCATC 1 cut(s) 1117
SfoI GGCGCC 1 cut(s) 618
SinI GGWCC 1 cut(s) 124
SmlI CTYRAG 1 cut(s) 867
SmoI CTYRAG 1 cut(s) 867
Sse9I AATT 2 cut(s) 52, 290
SspDI GGCGCC 1 cut(s) 616
SspMI CTAG 2 cut(s) 680, 764
SstI GAGCTC 2 cut(s) 213, 1112
StyD4I CCNGG 1 cut(s) 723
StyI CCWWGG 3 cut(s) 42, 108, 1066
TaaI ACNGT 5 cut(s) 15, 514, 544, 610, 631
TaiI ACGT 2 cut(s) 206, 1136
TaqI TCGA 1 cut(s) 263
TasI AATT 2 cut(s) 52, 290
TatI WGTACW 2 cut(s) 315, 912
TfiI GAWTC 6 cut(s) 137, 178, 448, 676, 970, 976
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TscAI CASTG 1 cut(s) 534
TseFI GTSAC 1 cut(s) 514
TseI GCWGC 6 cut(s) 113, 491, 640, 657, 715, 815
Tsp45I GTSAC 1 cut(s) 514
TspDTI ATGAA 1 cut(s) 259
TspGWI ACGGA 1 cut(s) 546
TspRI CASTG 1 cut(s) 534
Van91I CCANNNNNTGG 1 cut(s) 730
VpaK11BI GGWCC 1 cut(s) 124
XapI RAATTY 1 cut(s) 290
XbaI TCTAGA 1 cut(s) 679
XceI RCATGY 1 cut(s) 715
XmnI GAANNNNTTC 1 cut(s) 56
XspI CTAG 2 cut(s) 680, 764
ZrmI AGTACT 2 cut(s) 317, 914
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.