Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002992

Physical Location & Seq

Genomic Coordinates Forward (+)

9354959 .. 9366809

Gene Structure 11851 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1068800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4374 bp

ATGGACAGTGAATTGTATGAAGAAGAGCCAGTTCGATCTGGTATTATAACTGCCATACGTCTAAATGTTACAACTGGGGAAGATATTCTAAGGATATCTGAAGAAACACCAATCAGTTCAGCCAGTGAGGTCACTGATCCTAGGTTCGGACTTCCAAATCCATCTGGTCAGTGCTCTACTTGTGGTGCCGAAAACAAGTTACAGAACTGTGAAGGTCATTATGGGCGTATCCAGTTTCCATGCTCAATAGTCCATCCATATTATCTTCCGGAAGTTGTTCAAATCTTAAACAAGATTTGTGTAGGATGTAAATCTGTTAAGCAGGATCTAAAGCCCAAGGGTGCTGATTCTCTAAGTGGTAAGAGAAAGCGCCAGGAGCACAAGGAGCGCAAGGGCTGCAAGTATTGTAAGGGAAGTTCGTTAAGCTACCCAAGAATGAAGTTCAGAGTTTCTACGAGAGAACTCTTTAAAAGAAGTTGTATTATCGTGGAAGCAAGCGGAATATTGCCTTCTGACTATTGGGATTTTATTCCCAAAGATGCACAGCAAGACGAGAGTTGCGTTAAACCAAACAAAAGAATTCTATCTCATGCCCAGGTTCATCATTTGTTGAAGGATGTTGATCCAAATTTCGTTGCGAAGTTTGTCCCAATAACAGATTCACTTTCTATGGATTCTTTCCTTGTAACTCCAAATTGTCATCGCGTGACAGAATTTATGCATGCTTTTGCTAATGGGCAGCGGTTGATGTTTGATCATCGGACCACGGCTTATAGGAAACTTGTTGATTTCAAAGGTGTAGCTAATGAGCTGGGTTCTCGTTTGAACTCTGAGAAACCGGGCAATGATTTGATTGCCGCTCAGCTAAAAAATGTCAAAGACGATCCTTCAAAATCATCTGGTTTGAGATGGATCAAAGATGTCTTTCTTGGGAAGCGAACTGATCACTGTTTTCGCACGGTGGTGGTTGGTGATCCAAACATAAAGCTCAGTGAAATTGGTATACCCTCAAATATTGCAGAGAGGATGCATATAGCGGAGAATCTGAACAGATGGAACTTAGAAAGGTTGGGTGCCACCTGCAATCTACTTTTAATGTTCAAACAAGTGATGCATGTTCGTAGAAAAGGTAGCATCGTTCTGGTACACCAGACAGATGAACTGCAGATGGGAGATGCTATTTACAGGCCTCTGAGTGATGGAGACATTGTGTTGATAAATAGGCCTCCATCGATACATCAACACTCTCTCATTGCTTTATTCGTCAAGATACTTCCGGCAGCGCCTGTTGTATCTATAAACCCACTCTGTTGCTCTCCATTTCGTGGTGATTTTGATGGTGATTGCCTTCATGGCTATGTTCCTCAATCAGTTGATGCCAAAGTTGAGCTTAGGGAGCTTGTTGCTTTAGATAAGCAATTGATTAATGGGCAGAGCGGTACCAACCTACTTTCACTTAGTCAGGATAGCTTAACGGCTGCCCATCTGATCCTGGAGGATGGTGTTCTCTTGAATCGTTCTCAAATGCAACAGTTGCAAAGGTTCTGTTGCAATGAACTGCCATCACCGGCAATTATTAAAGCTCCTTCACTTAATGGCCCTGCTTGGACCGGAAAGCAAGTTTTCAGCATGCTCCTACCTTTGGGTTTTGATTATGATTTTGCTCCAGATGGAGTTTGCGTTAGAAATGGGGAGCTGCTATCTTCTGAAGGTTCTTCATGGTTGCGAGATATGAGTGGAAATCTTTATCAGAGTCTCATTAAACATTGCCAGGGTCCGGTACTTGACATCCTGCATGCTGCTCAGGAAGTTTTTTGTGAGTGGTTGTCAATGAGGGGCTTGACTGTCTCTCTCTCGGACTTATACCTCGCTTCTGACTCATGTTCAAGGAATAACTTGATGGAGGAAATTTTCTGTGGGTTGCGAGAAGCAGAGCAGGCATGTATATTTAGACAGTTGTTGGTGGATAGTAGTCGGGAACTTCTTATGGGATGTGCTGAAGAGAATCAAAGTCCCGTGACTTCGGATGTCGACTACTTCTGTCATGAGAAGCAGAAATCTGCTGCCCTCAGTCAGGTTGTTCACGATGCTTTCAAGCAATTTTTCCGGGATATTCAAAATTTAGCTTACAAATATGCCGGTACGGACAACTCTTTGATGAATATGCTCAAGGCTGGAAGCAAGGGTAATATGCAGAAATTAGTGCAGCACAGTATGTGCCTTGGTTTGCAAAACTCACTAGTTCCATTATCATTCAGAATTCCCAACCAGCTCTCGTGTGCTGCATGGAATAATCAAAAGGCAATCAGTTCAGATCAGAAGGTTGAGGGAAGTTCTGAATGTGTTGAGTCCTACATCCCCTCTGCCGTGGTTGAAAACTCTTTCTTGACAGGCTTAAATCCATTAGAATGTTTCATTCATTCAGTAACAAGTCGAGATAGTTCTTTTAGTGGAAATGCTGATCTTCCTGGGACTTTGACACGAAAGCTTATGTTTTTCATGAGGGATTTGTGCACTGCTTATGATGGAACGGTGAGAAATGCATATGGGAACAAGGTTGTTCAGTTCTCCTATGACATATCCTCTCCAGATAGTGCTAGTACTGGAGATGACGAGAGTATTACTGCTTGTGATGGTGTAGGTGGTCAACCTGTTGGCTCATTGTCTGCCTGTGCCATATCTGAAGCTGCATACAGCGCTCTAGATCAGCCAGTTAGTCTTCTCGAAACTTCTCCACTTCTAAATCTGAAGAATATCTTGGAATGTGGTTCAAAGAAAAGCAGTGCCAAGCAGACCATGTCTCTATATTTATCTGAGAAGCTTGGAAGACATAGGCATGGTTTCGAGTATGCAGCCTTGGAAGTTAAGAACTATTTGGAAGGGCTGAAATTTTCCGATATTGTTTCTACGGTCCTGATAATCTTCTCCTCCCAAAACGGGAGTAAAAAACGTTTCAGCCCATGGGTTACCCACTTTCACATAGGCAAGGAAATTGTGGGGAGGAGGAGACTGAAGATGCATTCCATTGTCAATTCCCTTTATGCTAGGTGCAACTCTGCCAGACCAGAACTAAAAGCTATTCTTCGCAGTTTGCTGATTACGAGCAAGGACTGTTCTGAAGCTCACACATGCAAGGGAGATAACGACACGTTTTGCATCACTGTTACTATGGCTGAAAACCACAAAAGTTCTTCTGAACAGCTGGATAAAGCTCGAGACTTGGTGATACCTTTCCTGCTTGAAACAGTAGTTAAAGGGTTATTGGAGGTGAAAAAGGTGGATATTTTGTGGAATGACCAACCCAAACGATCAAAAGTAGCTGATGGTTCATCCGGTGAACTGTACTTGAAGGTTTCTATGGTAGGAAAGAGCGGCAAGAAAAGACTTTGGAGTATGCTTTTGGATAGTTGTATCCAGATTATGAATGTGATTGATTGGTCACGAAGTCATCCCGATAATGTTCATGAGTATTGCCTTGCCTATGGAATTGATGTTGGGTGGAACTATTTCCTCAATAGTTTAGAGAGCACATGCAATGACATTGGAAAGAACATACTCCCTGAACATTTGATCCTTGTAGCAGATTGCCTCACATGCACAGGAGAATTTGTGGGCTTAAATGCCAAGGGCATTGCCCAACAACAAGAACACGCATCTGTATCATCTCCATTCATGCAAGCGTGTTTCTCTACTCCCGGGGCTTGCTTTGTAAAAGCAGCCAAGGCTGGAGCCATGGATGAACTTCCGGGGAGTTTGGAAGCCTTGGCATGGGGAAAGCTTCCTCCTTCTGGGACTGGCAGGCAATTTGAGGTTTTATTTTCTCAAAAGGGGCTCAACTTGTCTAAGCCACTTGATGTTTATGACATGCTTCGATGCCAAATTGGTTCCATTTGCATTAAGGAGAATGAAGTGAAAATGCCTGAAGTTTCGAACAATTCACTCGACAAATGTGGTTCTCCATATCTGCTCCAAAATGCAAAACACGTTGGGCTTGAGAAGTTTGAGAGTATATCCAAATCAGTCATTAAAAAGTATCTCAAGTTCAATGATATCCTCAAGTGGTCTCAGACGTGGAGGCACATACTGCGCAAGTATCCAATCAATGAGACGTTAAATGATGAGGACAAGTCAAAATTGATGACGGCTTTATATTTTCATCCTCATAGAATGGATAAGATTGGACCTGGAGTTCAAGACATAAAGATCGGCGCTCACCCTAAACATGAGGAGGCACACTGCTTTATGGTGGTACGAACAGATGGAACTGTTGAAGATTTTTCGTACCACAAGTGTGTGATGGGTGCTCTTGAGATTGTAGCACCTCAGAGGGTTAAGAGCTACCAGGCAAAATGGTTTGGCCGCGGACCTGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000418 GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010495 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901698 GO:1901699 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1458

Amino Acids

162.08

Weight (kDa)

7.45

Isoelectric Point (pI)

45.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	39 - 208	1.2e-09	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	311 - 468	3.4e-32	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	473 - 621	2.8e-18	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	686 - 746	7.4e-09	RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5	PF04998	798 - 1216	8.4e-09	RNA polymerase Rpb1, domain 5
DUF3223	PF11523	1359 - 1432	5.5e-26	Protein of unknown function (DUF3223)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002992)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63020 AT1G63020 AT1G63020 AT1G63020 AT1G63020
fragaria_vesca	FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400
malus_domestica	MD04G1068800.v1.1 MD06G1069300.v1.1 MD06G1069400.v1.1 MD06G1069500.v1.1 MD06G1069800.v1.1
prunus_persica	Prupe.5G089300_v2.0.a1
pyrus_communis	pycom04g06250
rosa_chinensis	RchiOBHm_Chr7g0199481
rosa_laevigata	RLG00000003790
rosa_multiflora	Rmu_co8411067.1_g000001 Rmu_sc0003890.1_g000001
rosa_roxburghii	Rroxscaffold_3G00256700
rosa_rugosa	Rorug07G0054300 Rorug07G0054400 Rorug07G0054500 Rorug07G0054500
rosa_samantha	Rh7AG179300 Rh7BG182800 Rh7CG188100 Rh7DG182200
rosa_wichuraiana	Rw7G015760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	47
AarI	CACCTGC	1 cut(s)	1088
Acc16I	TGCGCA	1 cut(s)	4088
Acc36I	ACCTGC	1 cut(s)	1088
Acc65I	GGTACC	1 cut(s)	1439
AccB1I	GGYRCC	3 cut(s)	185, 1073, 1439
AccB7I	CCANNNNNTGG	1 cut(s)	1325
AccBSI	CCGCTC	3 cut(s)	860, 1437, 3372
AccI	GTMKAC	2 cut(s)	1003, 2031
AccII	CGCG	2 cut(s)	705, 4362
AccIII	TCCGGA	1 cut(s)	268
AciI	CCGC	8 cut(s)	498, 742, 858, 1037, 1437, 3372, 4360, 4362
AclI	AACGTT	1 cut(s)	2950
AclWI	GGATC	8 cut(s)	131, 333, 617, 878, 920, 968, 1483, 3565
AcoI	YGGCCR	1 cut(s)	4357
AcsI	RAATTY	8 cut(s)	579, 628, 713, 1908, 2119, 2259, 2888, 3605
AcuI	CTGAAG	8 cut(s)	120, 1728, 2019, 2703, 2768, 3032, 3138, 3942
AfaI	GTAC	8 cut(s)	1146, 1441, 1782, 2143, 2602, 3344, 4251, 4283
AfeI	AGCGCT	1 cut(s)	2698
AfiI	CCNNNNNNNGG	8 cut(s)	146, 1325, 1642, 1777, 2074, 2937, 3768, 3788
AflIII	ACRYGT	2 cut(s)	3147, 3982
AhlI	ACTAGT	1 cut(s)	2239
AjiI	CACGTC	1 cut(s)	4071
AjnI	CCWGG	7 cut(s)	372, 594, 1491, 1770, 2467, 4183, 4341
AjuI	GAANNNNNNNTTGG	4 cut(s)	643, 675, 2741, 2773
AleI	CACNNNNGTG	2 cut(s)	962, 4290
AloI	GAACNNNNNNTCC	4 cut(s)	3555, 3587, 4173, 4205
Alw21I	GWGCWC	5 cut(s)	176, 381, 2516, 3530, 4306
Alw26I	GTCTC	8 cut(s)	1197, 1760, 1852, 2805, 3001, 3210, 4068, 4100
Alw44I	GTGCAC	1 cut(s)	2512
AlwI	GGATC	8 cut(s)	131, 333, 617, 878, 920, 968, 1483, 3565
AlwNI	CAGNNNCTG	1 cut(s)	1286
Ama87I	CYCGRG	2 cut(s)	3213, 3695
Aor13HI	TCCGGA	1 cut(s)	268
Aor51HI	AGCGCT	1 cut(s)	2698
AoxI	GGCC	4 cut(s)	1187, 1223, 1597, 4357
ApaLI	GTGCAC	1 cut(s)	2512
ApoI	RAATTY	8 cut(s)	579, 628, 713, 1908, 2119, 2259, 2888, 3605
AseI	ATTAAT	1 cut(s)	1425
Asp700I	GAANNNNTTC	3 cut(s)	84, 276, 3506
Asp718I	GGTACC	1 cut(s)	1439
AspA2I	CCTAGG	1 cut(s)	140
AspLEI	GCGC	6 cut(s)	372, 390, 1285, 2699, 4089, 4211
AspS9I	GGNCC	7 cut(s)	762, 1598, 1608, 1775, 2911, 4181, 4364
AsuC2I	CCSGG	5 cut(s)	840, 2108, 3696, 3697, 3747
AsuHPI	GGTGA	9 cut(s)	983, 1340, 1352, 1557, 2545, 3235, 3280, 3347, 4205
AsuII	TTCGAA	1 cut(s)	3929
AvaI	CYCGRG	2 cut(s)	3213, 3695
AvaII	GGWCC	6 cut(s)	762, 1608, 1775, 2911, 4181, 4364
AvrII	CCTAGG	1 cut(s)	140
BaeGI	GKGCMC	1 cut(s)	2516
BanI	GGYRCC	3 cut(s)	185, 1073, 1439
BanII	GRGCYC	1 cut(s)	3834
BauI	CACGAG	1 cut(s)	2275
BbsI	GAAGAC	2 cut(s)	2711, 2833
Bbv12I	GWGCWC	5 cut(s)	176, 381, 2516, 3530, 4306
BceAI	ACGGC	4 cut(s)	783, 1491, 2351, 4158
BciT130I	CCWGG	7 cut(s)	374, 596, 1493, 1772, 2469, 4185, 4343
BciVI	GTATCC	3 cut(s)	239, 3422, 4104
BclI	TGATCA	2 cut(s)	754, 943
BcnI	CCSGG	5 cut(s)	840, 2108, 3696, 3697, 3747
BcoDI	GTCTC	8 cut(s)	1197, 1760, 1852, 2805, 3001, 3210, 4068, 4100
BcuI	ACTAGT	1 cut(s)	2239
BfaI	CTAG	5 cut(s)	141, 2240, 2598, 2702, 3045
BfmI	CTRYAG	1 cut(s)	1163
BfoI	RGCGCY	4 cut(s)	373, 1286, 2700, 4212
BfuAI	ACCTGC	1 cut(s)	1088
BfuI	GTATCC	3 cut(s)	239, 3422, 4104
BglI	GCCNNNNNGGC	1 cut(s)	1353
BlnI	CCTAGG	1 cut(s)	140
BlpI	GCTNAGC	1 cut(s)	861
BmcAI	AGTACT	1 cut(s)	2602
Bme18I	GGWCC	6 cut(s)	762, 1608, 1775, 2911, 4181, 4364
BmeT110I	CYCGRG	2 cut(s)	3213, 3695
BmgBI	CACGTC	1 cut(s)	4071
BmgT120I	GGNCC	7 cut(s)	762, 1598, 1608, 1775, 2911, 4181, 4364
BmiI	GGNNCC	6 cut(s)	187, 1075, 1441, 1776, 3730, 3886
BmrI	ACTGGG	1 cut(s)	84
BmuI	ACTGGG	1 cut(s)	84
BpiI	GAAGAC	2 cut(s)	2711, 2833
BpmI	CTGGAG	6 cut(s)	1514, 1650, 2571, 2625, 3747, 4206
Bpu10I	CCTNAGC	2 cut(s)	1391, 1803
Bpu1102I	GCTNAGC	1 cut(s)	861
Bpu14I	TTCGAA	1 cut(s)	3929
BpuEI	CTTGAG	5 cut(s)	2153, 4013, 4022, 4040, 4328
BpuMI	CCSGG	5 cut(s)	840, 2108, 3696, 3697, 3747
Bsa29I	ATCGAT	1 cut(s)	1232
BsaBI	GATNNNNATC	2 cut(s)	310, 621
BsaI	GGTCTC	1 cut(s)	4068
BsaWI	WCCGGW	4 cut(s)	268, 1610, 1777, 3332
BsaXI	ACNNNNNCTCC	6 cut(s)	1194, 1224, 1665, 1695, 3594, 3624
Bsc4I	CCNNNNNNNGG	8 cut(s)	146, 1325, 1642, 1777, 2074, 2937, 3768, 3788
Bse118I	RCCGGY	2 cut(s)	1567, 2138
Bse1I	ACTGG	7 cut(s)	29, 79, 123, 232, 2608, 2711, 3799
Bse3DI	GCAATG	6 cut(s)	850, 1251, 1558, 1765, 3541, 3630
Bse8I	GATNNNNATC	2 cut(s)	310, 621
BseAI	TCCGGA	1 cut(s)	268
BseBI	CCWGG	7 cut(s)	374, 596, 1493, 1772, 2469, 4185, 4343
BseCI	ATCGAT	1 cut(s)	1232
BseJI	GATNNNNATC	2 cut(s)	310, 621
BseLI	CCNNNNNNNGG	8 cut(s)	146, 1325, 1642, 1777, 2074, 2937, 3768, 3788
BseMI	GCAATG	6 cut(s)	850, 1251, 1558, 1765, 3541, 3630
BseNI	ACTGG	7 cut(s)	29, 79, 123, 232, 2608, 2711, 3799
BseRI	GAGGAG	4 cut(s)	2917, 3016, 3019, 4241
BseSI	GKGCMC	1 cut(s)	2516
BseYI	CCCAGC	1 cut(s)	811
BsgI	GTGCAG	1 cut(s)	2225
Bsh1236I	CGCG	2 cut(s)	705, 4362
BshFI	GGCC	4 cut(s)	1189, 1225, 1599, 4359
BshNI	GGYRCC	3 cut(s)	185, 1073, 1439
BshVI	ATCGAT	1 cut(s)	1232
BsiHKAI	GWGCWC	5 cut(s)	176, 381, 2516, 3530, 4306
BsiHKCI	CYCGRG	2 cut(s)	3213, 3695
BslFI	GGGAC	4 cut(s)	632, 1998, 2485, 3805
BslI	CCNNNNNNNGG	8 cut(s)	146, 1325, 1642, 1777, 2074, 2937, 3768, 3788
BsmAI	GTCTC	8 cut(s)	1197, 1760, 1852, 2805, 3001, 3210, 4068, 4100
BsmBI	CGTCTC	1 cut(s)	4100
BsmFI	GGGAC	4 cut(s)	632, 1998, 2485, 3805
BsmI	GAATGC	1 cut(s)	3019
BsnI	GGCC	4 cut(s)	1189, 1225, 1599, 4359
Bso31I	GGTCTC	1 cut(s)	4068
BsoBI	CYCGRG	2 cut(s)	3213, 3695
Bsp119I	TTCGAA	1 cut(s)	3929
Bsp1286I	GDGCHC	6 cut(s)	176, 381, 2516, 3530, 3834, 4306
Bsp13I	TCCGGA	1 cut(s)	268
Bsp1720I	GCTNAGC	1 cut(s)	861
Bsp19I	CCATGG	2 cut(s)	2960, 3732
BspACI	CCGC	8 cut(s)	498, 742, 858, 1037, 1437, 3372, 4360, 4362
BspANI	GGCC	4 cut(s)	1189, 1225, 1599, 4359
BspDI	ATCGAT	1 cut(s)	1232
BspEI	TCCGGA	1 cut(s)	268
BspFNI	CGCG	2 cut(s)	705, 4362
BspHI	TCATGA	3 cut(s)	2044, 2499, 3463
BspLI	GGNNCC	6 cut(s)	187, 1075, 1441, 1776, 3730, 3886
BspMAI	CTGCAG	1 cut(s)	1167
BspMI	ACCTGC	1 cut(s)	1088
BspPI	GGATC	8 cut(s)	131, 333, 617, 878, 920, 968, 1483, 3565
BspQI	GCTCTTC	1 cut(s)	18
BspT104I	TTCGAA	1 cut(s)	3929
BspT107I	GGYRCC	3 cut(s)	185, 1073, 1439
BspTNI	GGTCTC	1 cut(s)	4068
BsrBI	CCGCTC	3 cut(s)	860, 1437, 3372
BsrDI	GCAATG	6 cut(s)	850, 1251, 1558, 1765, 3541, 3630
BsrFI	RCCGGY	2 cut(s)	1567, 2138
BsrI	ACTGG	7 cut(s)	29, 79, 123, 232, 2608, 2711, 3799
BssAI	RCCGGY	2 cut(s)	1567, 2138
BssNAI	GTATAC	1 cut(s)	1004
BssSI	CACGAG	1 cut(s)	2275
BssT1I	CCWWGG	9 cut(s)	140, 336, 2221, 2856, 2960, 3624, 3720, 3732, 3762
Bst1107I	GTATAC	1 cut(s)	1004
Bst2BI	CACGAG	1 cut(s)	2275
Bst2UI	CCWGG	7 cut(s)	374, 596, 1493, 1772, 2469, 4185, 4343
Bst6I	CTCTTC	2 cut(s)	18, 1995
BstAPI	GCANNNNNTGC	3 cut(s)	396, 1534, 4084
BstBI	TTCGAA	1 cut(s)	3929
BstC8I	GCNNGC	8 cut(s)	496, 723, 1631, 1797, 1938, 3678, 3703, 3800
BstDSI	CCRYGG	5 cut(s)	765, 2367, 2960, 3732, 4360
BstEII	GGTNACC	1 cut(s)	2965
BstENI	CCTNNNNNAGG	1 cut(s)	2072
BstFNI	CGCG	2 cut(s)	705, 4362
BstH2I	RGCGCY	4 cut(s)	373, 1286, 2700, 4212
BstHHI	GCGC	6 cut(s)	372, 390, 1285, 2699, 4089, 4211
BstMAI	GTCTC	8 cut(s)	1197, 1760, 1852, 2805, 3001, 3210, 4068, 4100
BstNI	CCWGG	7 cut(s)	374, 596, 1493, 1772, 2469, 4185, 4343
BstNSI	RCATGY	9 cut(s)	725, 1118, 1633, 1799, 1944, 3132, 3534, 3597, 3868
BstPI	GGTNACC	1 cut(s)	2965
BstSFI	CTRYAG	1 cut(s)	1163
BstSLI	GKGCMC	1 cut(s)	2516
BstUI	CGCG	2 cut(s)	705, 4362
BstV2I	GAAGAC	2 cut(s)	2711, 2833
BstX2I	RGATCY	1 cut(s)	325
BstYI	RGATCY	1 cut(s)	325
BstZ17I	GTATAC	1 cut(s)	1004
Bsu15I	ATCGAT	1 cut(s)	1232
BsuI	GTATCC	3 cut(s)	239, 3422, 4104
BsuRI	GGCC	4 cut(s)	1189, 1225, 1599, 4359
BsuTUI	ATCGAT	1 cut(s)	1232
BtgI	CCRYGG	5 cut(s)	765, 2367, 2960, 3732, 4360
BtgZI	GCGATG	1 cut(s)	686
BtrI	CACGTC	1 cut(s)	4071
BtsI	GCAGTG	3 cut(s)	2514, 2788, 4234
BveI	ACCTGC	1 cut(s)	1088
Cac8I	GCNNGC	8 cut(s)	496, 723, 1631, 1797, 1938, 3678, 3703, 3800
CaiI	CAGNNNCTG	1 cut(s)	1286
CciI	TCATGA	3 cut(s)	2044, 2499, 3463
CfoI	GCGC	6 cut(s)	372, 390, 1285, 2699, 4089, 4211
Cfr10I	RCCGGY	2 cut(s)	1567, 2138
Cfr13I	GGNCC	7 cut(s)	762, 1598, 1608, 1775, 2911, 4181, 4364
Cfr42I	CCGCGG	1 cut(s)	4363
Cfr9I	CCCGGG	1 cut(s)	3695
ClaI	ATCGAT	1 cut(s)	1232
Csp6I	GTAC	8 cut(s)	1145, 1440, 1781, 2142, 2601, 3343, 4250, 4282
CviQI	GTAC	8 cut(s)	1145, 1440, 1781, 2142, 2601, 3343, 4250, 4282
DraI	TTTAAA	1 cut(s)	469
EaeI	YGGCCR	1 cut(s)	4357
Eam1104I	CTCTTC	2 cut(s)	18, 1995
EarI	CTCTTC	2 cut(s)	18, 1995
Eco130I	CCWWGG	9 cut(s)	140, 336, 2221, 2856, 2960, 3624, 3720, 3732, 3762
Eco147I	AGGCCT	2 cut(s)	1189, 1225
Eco24I	GRGCYC	1 cut(s)	3834
Eco31I	GGTCTC	1 cut(s)	4068
Eco32I	GATATC	2 cut(s)	96, 4051
Eco47I	GGWCC	6 cut(s)	762, 1608, 1775, 2911, 4181, 4364
Eco47III	AGCGCT	1 cut(s)	2698
Eco57I	CTGAAG	8 cut(s)	120, 1728, 2019, 2703, 2768, 3032, 3138, 3942
Eco88I	CYCGRG	2 cut(s)	3213, 3695
Eco91I	GGTNACC	1 cut(s)	2965
EcoNI	CCTNNNNNAGG	1 cut(s)	2072
EcoO65I	GGTNACC	1 cut(s)	2965
EcoRI	GAATTC	2 cut(s)	579, 2259
EcoRII	CCWGG	7 cut(s)	372, 594, 1491, 1770, 2467, 4183, 4341
EcoRV	GATATC	2 cut(s)	96, 4051
EcoT14I	CCWWGG	9 cut(s)	140, 336, 2221, 2856, 2960, 3624, 3720, 3732, 3762
EcoT22I	ATGCAT	5 cut(s)	723, 1032, 1116, 2545, 3021
EcoT38I	GRGCYC	1 cut(s)	3834
ErhI	CCWWGG	9 cut(s)	140, 336, 2221, 2856, 2960, 3624, 3720, 3732, 3762
Esp3I	CGTCTC	1 cut(s)	4100
FalI	AAGNNNNNCTT	6 cut(s)	1374, 1406, 2741, 2773, 3066, 3098
FaqI	GGGAC	4 cut(s)	632, 1998, 2485, 3805
FauNDI	CATATG	1 cut(s)	2545
FbaI	TGATCA	2 cut(s)	754, 943
FblI	GTMKAC	2 cut(s)	1003, 2031
FriOI	GRGCYC	1 cut(s)	3834
FspBI	CTAG	5 cut(s)	141, 2240, 2598, 2702, 3045
FspI	TGCGCA	1 cut(s)	4088
GlaI	GCGC	6 cut(s)	371, 389, 1284, 2698, 4088, 4210
GsaI	CCCAGC	1 cut(s)	815
GsuI	CTGGAG	6 cut(s)	1514, 1650, 2571, 2625, 3747, 4206
HaeII	RGCGCY	4 cut(s)	373, 1286, 2700, 4212
HaeIII	GGCC	4 cut(s)	1189, 1225, 1599, 4359
HhaI	GCGC	6 cut(s)	372, 390, 1285, 2699, 4089, 4211
Hin6I	GCGC	6 cut(s)	370, 388, 1283, 2697, 4087, 4209
HinP1I	GCGC	6 cut(s)	370, 388, 1283, 2697, 4087, 4209
HincII	GTYRAC	2 cut(s)	2032, 2648
HindII	GTYRAC	2 cut(s)	2032, 2648
HindIII	AAGCTT	3 cut(s)	2486, 2819, 3776
HinfI	GANTC	9 cut(s)	347, 659, 674, 1042, 1513, 1753, 1877, 2005, 2348
HphI	GGTGA	9 cut(s)	983, 1340, 1352, 1557, 2545, 3235, 3280, 3347, 4205
Hpy166II	GTNNAC	7 cut(s)	1004, 1147, 2032, 2083, 2514, 2648, 3338
Hpy8I	GTNNAC	7 cut(s)	1004, 1147, 2032, 2083, 2514, 2648, 3338
HpyCH4IV	ACGT	6 cut(s)	58, 2950, 3149, 3984, 4070, 4109
HpySE526I	ACGT	6 cut(s)	58, 2950, 3149, 3984, 4070, 4109
HspAI	GCGC	6 cut(s)	370, 388, 1283, 2697, 4087, 4209
Kpn2I	TCCGGA	1 cut(s)	268
KpnI	GGTACC	1 cut(s)	1443
Ksp22I	TGATCA	2 cut(s)	754, 943
KspI	CCGCGG	1 cut(s)	4363
LguI	GCTCTTC	1 cut(s)	18
LmnI	GCTCC	9 cut(s)	376, 385, 1396, 1588, 1638, 1669, 1693, 3728, 3972
MaeI	CTAG	5 cut(s)	141, 2240, 2598, 2702, 3045
MaeII	ACGT	6 cut(s)	58, 2950, 3149, 3984, 4070, 4109
MbiI	CCGCTC	3 cut(s)	860, 1437, 3372
MfeI	CAATTG	1 cut(s)	1418
MflI	RGATCY	1 cut(s)	325
MhlI	GDGCHC	6 cut(s)	176, 381, 2516, 3530, 3834, 4306
MlyI	GAGTC	3 cut(s)	1762, 1871, 2357
Mph1103I	ATGCAT	5 cut(s)	723, 1032, 1116, 2545, 3021
MroI	TCCGGA	1 cut(s)	268
MroXI	GAANNNNTTC	3 cut(s)	84, 276, 3506
MslI	CAYNNNNRTG	5 cut(s)	962, 1356, 2407, 2835, 4290
MspA1I	CMGCKG	3 cut(s)	742, 3202, 4362
MunI	CAATTG	1 cut(s)	1418
Mva1269I	GAATGC	1 cut(s)	3019
MvaI	CCWGG	7 cut(s)	374, 596, 1493, 1772, 2469, 4185, 4343
MvnI	CGCG	2 cut(s)	705, 4362
NciI	CCSGG	5 cut(s)	840, 2108, 3696, 3697, 3747
NcoI	CCATGG	2 cut(s)	2960, 3732
NdeI	CATATG	1 cut(s)	2545
NlaIV	GGNNCC	6 cut(s)	187, 1075, 1441, 1776, 3730, 3886
NmuCI	GTSAC	4 cut(s)	130, 706, 2017, 3438
NsbI	TGCGCA	1 cut(s)	4088
NsiI	ATGCAT	5 cut(s)	723, 1032, 1116, 2545, 3021
NspI	RCATGY	9 cut(s)	725, 1118, 1633, 1799, 1944, 3132, 3534, 3597, 3868
NspV	TTCGAA	1 cut(s)	3929
OliI	CACNNNNGTG	2 cut(s)	962, 4290
PaeI	GCATGC	3 cut(s)	725, 1633, 1799
PaeR7I	CTCGAG	1 cut(s)	3213
PagI	TCATGA	3 cut(s)	2044, 2499, 3463
PaqCI	CACCTGC	1 cut(s)	1088
PceI	AGGCCT	2 cut(s)	1189, 1225
PciSI	GCTCTTC	1 cut(s)	18
PcsI	WCGNNNNNNNCGW	1 cut(s)	558
PctI	GAATGC	1 cut(s)	3019
PdmI	GAANNNNTTC	3 cut(s)	84, 276, 3506
PfeI	GAWTC	6 cut(s)	347, 659, 674, 1042, 1513, 2005
PflFI	GACNNNGTC	1 cut(s)	2797
PflMI	CCANNNNNTGG	1 cut(s)	1325
PfoI	TCCNGGA	2 cut(s)	1491, 2106
PleI	GAGTC	3 cut(s)	1761, 1871, 2356
PpsI	GAGTC	3 cut(s)	1761, 1871, 2356
PshBI	ATTAAT	1 cut(s)	1425
PsiI	TTATAA	1 cut(s)	47
Psp1406I	AACGTT	1 cut(s)	2950
Psp6I	CCWGG	7 cut(s)	372, 594, 1491, 1770, 2467, 4183, 4341
PspEI	GGTNACC	1 cut(s)	2965
PspFI	CCCAGC	1 cut(s)	811
PspGI	CCWGG	7 cut(s)	372, 594, 1491, 1770, 2467, 4183, 4341
PspN4I	GGNNCC	6 cut(s)	187, 1075, 1441, 1776, 3730, 3886
PspPI	GGNCC	7 cut(s)	762, 1598, 1608, 1775, 2911, 4181, 4364
PsrI	GAACNNNNNNTAC	4 cut(s)	400, 432, 4025, 4057
PstI	CTGCAG	1 cut(s)	1167
PstNI	CAGNNNCTG	1 cut(s)	1286
PsuI	RGATCY	1 cut(s)	325
PsyI	GACNNNGTC	1 cut(s)	2797
PvuII	CAGCTG	1 cut(s)	3202
RsaI	GTAC	8 cut(s)	1146, 1441, 1782, 2143, 2602, 3344, 4251, 4283
RsaNI	GTAC	8 cut(s)	1145, 1440, 1781, 2142, 2601, 3343, 4250, 4282
RseI	CAYNNNNRTG	5 cut(s)	962, 1356, 2407, 2835, 4290
SacII	CCGCGG	1 cut(s)	4363
SalI	GTCGAC	1 cut(s)	2030
SapI	GCTCTTC	1 cut(s)	18
Sau96I	GGNCC	7 cut(s)	762, 1598, 1608, 1775, 2911, 4181, 4364
ScaI	AGTACT	1 cut(s)	2602
SchI	GAGTC	3 cut(s)	1762, 1871, 2357
SduI	GDGCHC	6 cut(s)	176, 381, 2516, 3530, 3834, 4306
SfcI	CTRYAG	1 cut(s)	1163
Sfr274I	CTCGAG	1 cut(s)	3213
Sfr303I	CCGCGG	1 cut(s)	4363
SfuI	TTCGAA	1 cut(s)	3929
SgrBI	CCGCGG	1 cut(s)	4363
SinI	GGWCC	6 cut(s)	762, 1608, 1775, 2911, 4181, 4364
SlaI	CTCGAG	1 cut(s)	3213
SmaI	CCCGGG	1 cut(s)	3697
SmiMI	CAYNNNNRTG	5 cut(s)	962, 1356, 2407, 2835, 4290
SmlI	CTYRAG	6 cut(s)	2168, 3213, 3992, 4037, 4055, 4307
SmoI	CTYRAG	6 cut(s)	2168, 3213, 3992, 4037, 4055, 4307
SpeI	ACTAGT	1 cut(s)	2239
SphI	GCATGC	3 cut(s)	725, 1633, 1799
SseBI	AGGCCT	2 cut(s)	1189, 1225
SsiI	CCGC	8 cut(s)	498, 742, 858, 1037, 1437, 3372, 4360, 4362
SspI	AATATT	2 cut(s)	504, 1015
SspMI	CTAG	5 cut(s)	141, 2240, 2598, 2702, 3045
StuI	AGGCCT	2 cut(s)	1189, 1225
StyI	CCWWGG	9 cut(s)	140, 336, 2221, 2856, 2960, 3624, 3720, 3732, 3762
TaiI	ACGT	6 cut(s)	61, 2953, 3152, 3987, 4073, 4112
TatI	WGTACW	2 cut(s)	2600, 3342
TauI	GCSGC	3 cut(s)	860, 3375, 4362
TfiI	GAWTC	6 cut(s)	347, 659, 674, 1042, 1513, 2005
TseFI	GTSAC	4 cut(s)	130, 706, 2017, 3438
Tsp45I	GTSAC	4 cut(s)	130, 706, 2017, 3438
TspGWI	ACGGA	1 cut(s)	2159
TspMI	CCCGGG	1 cut(s)	3695
Tth111I	GACNNNGTC	1 cut(s)	2797
Van91I	CCANNNNNTGG	1 cut(s)	1325
VneI	GTGCAC	1 cut(s)	2512
VpaK11BI	GGWCC	6 cut(s)	762, 1608, 1775, 2911, 4181, 4364
VspI	ATTAAT	1 cut(s)	1425
XagI	CCTNNNNNAGG	1 cut(s)	2072
XapI	RAATTY	8 cut(s)	579, 628, 713, 1908, 2119, 2259, 2888, 3605
XbaI	TCTAGA	1 cut(s)	2701
XceI	RCATGY	9 cut(s)	725, 1118, 1633, 1799, 1944, 3132, 3534, 3597, 3868
XhoI	CTCGAG	1 cut(s)	3213
XmaI	CCCGGG	1 cut(s)	3695
XmaJI	CCTAGG	1 cut(s)	140
XmiI	GTMKAC	2 cut(s)	1003, 2031
XmnI	GAANNNNTTC	3 cut(s)	84, 276, 3506
XspI	CTAG	5 cut(s)	141, 2240, 2598, 2702, 3045
ZrmI	AGTACT	1 cut(s)	2602
Zsp2I	ATGCAT	5 cut(s)	723, 1032, 1116, 2545, 3021

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.