Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0002992

Physical Location & Seq

Genomic Coordinates Forward (+)

16889544 .. 16903240

Gene Structure 13697 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG182200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4368 bp

ATGGAAGATGAATTGTATAATGAAGAACTAGTGCCATGTGCTTGCATAACTGGGGTACATCTAAATGTTTCCACTCGGGAAGATACGGAGAAAATATCAACATTATTAATCGAGGCAGCTGCCCAGGTTAATGATCCAAAGTTGGGACTTCCAAATCCAACGAATCAGTGTTCTACTTGTGGTACCCAAGATATAAAATCCTGTGATGGTCATTTTGGGTCCATCAAGTTTCCGTGCACGATACTCCATCCATACTATCTACCGGAAGTTGCTCTTATTTTAAACAAGATTTGCCCGGCATGTAAAACCATCAGGCAAGGTGTAAGGGTCAAGGGATCTGAACGGAGGGGCTGCAGATATTGCTTTGGAGGTTCATCGGATTACCCTAGAATGAAGTTCAAAGTTTCTTCTAGAGAGCTTTTCAGAAGAAGTACAATAATAGTAGAAGCAGTTGGAAAATTGCCTCCTGATTATTGGGACTTCATTCCCAAAGATCCCCAACATGATGAAACACACACAAAACCAAATAGAAGAGTTCTATCTCATGCCCAGGTTCATTTTTTGTTGAATGATGTTGATCCAGATTTCATCACAAAGTTTGTTCCAACAACCAGCTCACTTTCTTTGGACTGTTTCCTTGTCACACCAAATTGTCATCGTGTGACAGAAGTTATGTATTCATTTAATAATGGGCAGTCATTGATGTTTGATGATCGGACTAGAGCTTACAGGAAACTAGTGGATTTCAGAGGCACCGCAAACGAGCTGGGTTCTCGTGTTTTGGACTGCCTGAAAATTTCCAAGATAAACAAAGACAAAACAGGCAGAGATTCAATTTCAGTTGAGCAACAAAAGATTAAGGATTCTCCGTCCAGAACATCCGGCTTGAGATGGATAAAAGATGTTGTTCTTGGAAAGAGAAGTGATCACTGTTTTCGAACTGTTGTCGTTGGTGATCCAAACATCAAGCTGAGTGAGATTGGCATACCCCGCCATATTGCAGAGAAAATGCAGATCTCTGAGAATCTGAACCAATATAACCTTGACAAGTTGTATGCGTTTTGCCAGCTACGCCTTGGCCATCAGGGGAGAGGTGACATACATGTTCGTAGAAATGATAGTCTGGTTAGGATAAGTAATTTAGAAGAACTGGAGATGGGAGATATTATATACAGGGCGTTGAGTGATGGAGATGTTGTGTTGATAAATAGGCCTCCATCAATACATCAACACTCCCTAATAGCTTTAACTGCAAAGATCCTTCCAGTAAACTCTGTAGTGTCGATAAATCCACTGTGTTGCTCTCCTTTTCGTGGTGATTTTGATGGTGATTGCCTTCATGGTTATGTTCCTCAATCAGTGGATACCAGAGTCGAGCTTACAGAGCTTGTTGCTTTAGATAAGCAATTAGTTAATGGGCAGAGTGGTTCCAACCTTCTTTCTCTCAGTCAAGATAGTTTAACTGCTTGCTATCTCATCATGGAAGATGGCACCCTGATGAATAAGTTTCAAATGCAACAACTGAAAATGCTTTGTCCCCCTGAACTGCCATCACCAGCGATAATCAAAGATCCTTCTGGTAACTCCGTTTCTTGGACGGCAAAGCAGATAATCAGCATGTTTCTACCTGCAAATTTTAATTATGCATTTCCTTCAAATGGCGTGCATATTAGCAATGGAGAGCTTCTAGCTTCTTCTGAAGGTTCTTCTTGGCTGCGAGACACCAGTGGGAATCTTTTCCAAAGTCTCATCGAACATTGCCCCGGTCAGATACTGGACATCTTCTTTACTGCTCAGGGAGTTCTATGTGAGTGGTTGTCAATGAGGGGCTTGAGTGTTTCTCTCTCAGACTTGTATATCGCGTCGGATACATGTTCAAGGAAAAACTTGGTGGAGGAAATCTTCTATGGGTTACAAGAAGCAGAGGAGGCATGTACTATCAGACAGTTGATGCTTGATTCTTGTCAGGAATTTCTTATGGGCTATGCTGAAGAGACAAAAAGCCCTTTGACTTTTGGTTCAGAACATTTTTGTTATCAGAAGCAGAAATCTGCTGCACTTAGTCAGGTCACGGTAGATGCTTTCAAAGAAGGTTTCCGGGATGTCCAAAATTTAGCATACAAATATGCCCGTGAGGACAACTCGTTGCTGGCTATGTTTAAGGCTGGAAGCAAGGGTAATATGCTGAAATTAGTTCAGCATGGTATGTGCCTTGGTTTGCAGCATTCACTAGTTCCCTTATCATTCAAGTTTCCGAACCAGCTCTCATGTGATGCATGGAATGATCAAAAAGCACGTGGTATCGTTCAGGAGGTTGGAAGAGCTTTTGAATCCATCGAGTCATATATCCCATCCACTGTCGTTAAAAGTTCGTTCTTGACTGGGCTGAATCCGGTCGAATGCTTTGTTCATTCAGTCACGAGTCGAGATGGTTCTTTTAGTAATAATGCGGAACTTCCTGGGACTCTGAATCGAAAGCTCATGTATTTCATGCGTGATTTGCATACTGCTTATGACGGAACAGTTAGAAACGCATATGGGAACCAGCTGGTTCAGTTCTCCTATGATAATGACGAGGGCATATCTACTGCAGATAGCACCACAAACAGCTCATATGCAAAGAGTGTTATTGATTGTGGTGGCGGCCAACCCGTTGGTGCATTGTCTGCTTGTGCAATATCTGAAGCTGCATACAGTGCTTTAGATCAGCCAGTTAGTCTACTTGAAACTTCTCCTCTGCTAAATCTGAAGAACATTTTGGAGTGTGGTTCAAAGAAAAGGAGTGCTAAGCAAACTATGTCATTATTTTTATCTGAGAAGCTTGGAAGACCTAGGCATGGTTTCGAGTATGGAGCGCTAGAAGTTAAAAATCATCTAGAGGGACTCACATTTTCAGACATCGTTTCTACTGTCATGATAATGTTCTCCTCACAAACTGGCAGCAAGATGCATTTTAGTCCTTGGGTTTCTCACTTTCATATATCTAAGGAAATTGTACGAAGAAGAAGGCTGAATGTGCGATCTATTATAGAGGCACTTTATGCAAGATGCAACTCCGCTCCAGTGGAGTTGAAAATTATCCTTTGGAATTTGAAGATCACAAGAAATAAGGAATGTGATTGCTGCAGCACACCTAATGATACATTTTGCATCAGTGTCACACTGACTGAAAAGCAAGACAATTCTTCTGAAGAACTGGATAAAAAGCAAGACAAGTCTTCTGAAGAACTGGATAAAACTCGAGTCTTGGTGATACCATTCTTGCTTAAAACAGTAGTTAAAGGTTTTCTGGAGATAAAAAAGGTGGATATTATATGGAATGATCGAACTGGTTCACCTGGTGAACTCTACTTGAGAGTTTCTATGTCAGGAAAGACCAGAAGGGGGGCTACCTTTTGGAATATGCTTATGGACGATTGTCTTCCGGTTATGGATATGATTGATTGGTCACGAAGTCATCCAGATAACGTCGATGACTTCTGCACTGCCTATGGAATAGATGTTGGGTGGAAACATTTCCTTAATAAGTTGGAGTCTGCAACCGTAGATATTGGAAAGACAATTCTTCCGCAGCATTTGCTTCTTGCAGCAGATTGTCTGTCAGCCACCGGCGAGTTTGTCAGCTTAAACGCAAAAGGCATTGCTCAACAATTGGAACATGCATCTGTCACAGCCCCTTTTATGCAAGCTTGCTTTTCTACTCCTGGTCCTTGCTTGGTTAAAGCTGCTAAGGCTGGAGTGGTGGATAATCTCCAGGGGAGTTTGGACGCATTGACATGGGGAAATACTCCGTCCTTGGGAAGTGGTGGACAGTTTGATATTATATTTGCTGGGGAGGGATCTGTGTCTTCTAAGCCATTTGATGTTCATGATATGCTGAGCAGACAAATCAGTTCCAACCCCCAGAATGTATTTGAAATGTCTGATGCTCCAATTCATCTATCTGACAAATATGTTGCTCCACATGTGTACAAGTATGGTCTTTTGTGCAAGTTGAAGAGTATTACCAAATCAGTCTTAAGAGAAACTTTTACGATCCAGGATATTGATAGATTGTCTCAAACCTTGAAGAATATATTGCACAAGTATGACATCAATGAAAGCATAAATGAGATGGAGAAGAAAGATTTGATGACGGCTTTAGAATTTCACCCTCATAGGGATCGTAAGATCGGATTAGGAATCAAAGATATAAAGGTTGGCCAGCACCCTAACCATGAGAATGCACGATGCTTCATGCTAGTACATACGGATGGGACGATTGAAGATGTTTCGTACCACAAGTGCATCATTGGTGCTTTTGATATCATAGCTCCTCATAGGGCAAAGGGCTATCGCCTGAGACATTTGAGCCGTGGTCCTCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000418 GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010495 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901698 GO:1901699 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1455

Amino Acids

162.39

Weight (kDa)

6.63

Isoelectric Point (pI)

43.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	43 - 104	4.5e-07	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	305 - 464	5e-35	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	469 - 618	1.1e-19	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	687 - 742	3.8e-08	RNA polymerase Rpb1, domain 4
DUF3223	PF11523	1357 - 1430	5.2e-25	Protein of unknown function (DUF3223)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002992)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63020 AT1G63020 AT1G63020 AT1G63020 AT1G63020
fragaria_vesca	FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400
malus_domestica	MD04G1068800.v1.1 MD06G1069300.v1.1 MD06G1069400.v1.1 MD06G1069500.v1.1 MD06G1069800.v1.1
prunus_persica	Prupe.5G089300_v2.0.a1
pyrus_communis	pycom04g06250
rosa_chinensis	RchiOBHm_Chr7g0199481
rosa_laevigata	RLG00000003790
rosa_multiflora	Rmu_co8411067.1_g000001 Rmu_sc0003890.1_g000001
rosa_roxburghii	Rroxscaffold_3G00256700
rosa_rugosa	Rorug07G0054300 Rorug07G0054400 Rorug07G0054500 Rorug07G0054500
rosa_samantha	Rh7AG179300 Rh7BG182800 Rh7CG188100 Rh7DG182200
rosa_wichuraiana	Rw7G015760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1636
Acc65I	GGTACC	1 cut(s)	182
AccB1I	GGYRCC	3 cut(s)	182, 752, 1490
AccBSI	CCGCTC	1 cut(s)	3059
AccI	GTMKAC	1 cut(s)	2719
AccII	CGCG	1 cut(s)	1862
AciI	CCGC	6 cut(s)	756, 991, 2450, 2643, 3057, 3569
AcoI	YGGCCR	3 cut(s)	1078, 2644, 4201
AcsI	RAATTY	6 cut(s)	795, 1633, 1970, 2110, 3088, 4145
AcuI	CTGAAG	6 cut(s)	1719, 2010, 2703, 2768, 3210, 3243
AcvI	CACGTG	1 cut(s)	2297
AdeI	CACNNNGTG	1 cut(s)	3341
AfaI	GTAC	8 cut(s)	57, 184, 433, 1936, 2997, 3971, 4245, 4277
AfeI	AGCGCT	1 cut(s)	2856
AfiI	CCNNNNNNNGG	8 cut(s)	143, 1313, 1593, 1658, 2837, 3384, 3797, 4159
AflII	CTTAAG	1 cut(s)	4018
AflIII	ACRYGT	3 cut(s)	1102, 1871, 3964
AhlI	ACTAGT	3 cut(s)	28, 736, 2230
AjnI	CCWGG	7 cut(s)	123, 549, 2458, 3337, 3703, 3753, 4038
AjuI	GAANNNNNNNTTGG	2 cut(s)	2741, 2773
Alw21I	GWGCWC	1 cut(s)	239
Alw26I	GTCTC	5 cut(s)	1713, 1751, 1988, 4062, 4336
Alw44I	GTGCAC	1 cut(s)	235
AlwNI	CAGNNNCTG	1 cut(s)	1774
Ama87I	CYCGRG	2 cut(s)	75, 3240
Aor51HI	AGCGCT	1 cut(s)	2856
AoxI	GGCC	4 cut(s)	1078, 1211, 2644, 4201
ApaLI	GTGCAC	1 cut(s)	235
ApoI	RAATTY	6 cut(s)	795, 1633, 1970, 2110, 3088, 4145
AseI	ATTAAT	1 cut(s)	107
Asp700I	GAANNNNTTC	3 cut(s)	1736, 3331, 3515
Asp718I	GGTACC	1 cut(s)	182
AspA2I	CCTAGG	1 cut(s)	2831
AspLEI	GCGC	1 cut(s)	2857
AspS9I	GGNCC	3 cut(s)	219, 3707, 4358
AsuC2I	CCSGG	3 cut(s)	296, 1764, 2099
AsuHPI	GGTGA	9 cut(s)	965, 1106, 1328, 1340, 1545, 3262, 3327, 3353, 4142
AsuII	TTCGAA	1 cut(s)	937
AvaI	CYCGRG	2 cut(s)	75, 3240
AvaII	GGWCC	3 cut(s)	219, 3707, 4358
AvrII	CCTAGG	1 cut(s)	2831
BaeGI	GKGCMC	1 cut(s)	239
BaeI	ACNNNNGTAYC	2 cut(s)	1356, 1389
BalI	TGGCCA	2 cut(s)	1080, 4203
BanI	GGYRCC	3 cut(s)	182, 752, 1490
BarI	GAAGNNNNNNTAC	2 cut(s)	3373, 3405
BauI	CACGAG	2 cut(s)	774, 2419
BbrPI	CACGTG	1 cut(s)	2297
BbsI	GAAGAC	4 cut(s)	2833, 3210, 3413, 3840
Bbv12I	GWGCWC	1 cut(s)	239
BceAI	ACGGC	3 cut(s)	1614, 4152, 4338
BcgI	CGANNNNNNTGC	2 cut(s)	101, 135
BciT130I	CCWGG	7 cut(s)	125, 551, 2460, 3339, 3705, 3755, 4040
BciVI	GTATCC	2 cut(s)	1357, 1861
BclI	TGATCA	2 cut(s)	925, 2284
BcnI	CCSGG	3 cut(s)	296, 1764, 2099
BcoDI	GTCTC	5 cut(s)	1713, 1751, 1988, 4062, 4336
BcuI	ACTAGT	3 cut(s)	28, 736, 2230
BfmI	CTRYAG	4 cut(s)	352, 1275, 2589, 3124
BfoI	RGCGCY	1 cut(s)	2858
BfrI	CTTAAG	1 cut(s)	4018
BfuAI	ACCTGC	1 cut(s)	1636
BfuI	GTATCC	2 cut(s)	1357, 1861
BglII	AGATCT	1 cut(s)	1014
BlnI	CCTAGG	1 cut(s)	2831
BlpI	GCTNAGC	2 cut(s)	2787, 3878
Bme18I	GGWCC	3 cut(s)	219, 3707, 4358
BmeT110I	CYCGRG	2 cut(s)	75, 3240
BmgT120I	GGNCC	3 cut(s)	219, 3707, 4358
BmiI	GGNNCC	6 cut(s)	184, 220, 754, 1429, 1492, 2543
BmrI	ACTGGG	2 cut(s)	60, 2391
BmuI	ACTGGG	2 cut(s)	60, 2391
BoxI	GACNNNNGTC	1 cut(s)	3417
BpiI	GAAGAC	4 cut(s)	2833, 3210, 3413, 3840
BplI	GAGNNNNNCTC	4 cut(s)	1825, 1857, 2126, 2158
BpmI	CTGGAG	5 cut(s)	1172, 3045, 3311, 3737, 3756
Bpu10I	CCTNAGC	2 cut(s)	1794, 3729
Bpu1102I	GCTNAGC	2 cut(s)	2787, 3878
Bpu14I	TTCGAA	1 cut(s)	937
BpuEI	CTTGAG	3 cut(s)	907, 1852, 3373
BpuMI	CCSGG	3 cut(s)	296, 1764, 2099
BsaAI	YACGTR	1 cut(s)	2297
BsaBI	GATNNNNATC	1 cut(s)	576
BsaWI	WCCGGW	3 cut(s)	262, 2392, 3424
BsaXI	ACNNNNNCTCC	2 cut(s)	1182, 1212
Bsc4I	CCNNNNNNNGG	8 cut(s)	143, 1313, 1593, 1658, 2837, 3384, 3797, 4159
Bse118I	RCCGGY	1 cut(s)	3608
Bse3DI	GCAATG	3 cut(s)	1681, 1756, 3639
Bse8I	GATNNNNATC	1 cut(s)	576
BseBI	CCWGG	7 cut(s)	125, 551, 2460, 3339, 3705, 3755, 4040
BseGI	GGATG	6 cut(s)	247, 878, 2107, 2351, 3457, 4258
BseJI	GATNNNNATC	1 cut(s)	576
BseLI	CCNNNNNNNGG	8 cut(s)	143, 1313, 1593, 1658, 2837, 3384, 3797, 4159
BseMI	GCAATG	3 cut(s)	1681, 1756, 3639
BseMII	CTCAG	8 cut(s)	962, 1011, 1459, 1808, 1860, 2805, 3869, 4331
BseRI	GAGGAG	4 cut(s)	1940, 2724, 2917, 4305
BseSI	GKGCMC	1 cut(s)	239
BseYI	CCCAGC	2 cut(s)	766, 3830
BsgI	GTGCAG	2 cut(s)	2040, 3466
Bsh1236I	CGCG	1 cut(s)	1862
Bsh1285I	CGRYCG	1 cut(s)	2397
BshFI	GGCC	4 cut(s)	1080, 1213, 2646, 4203
BshNI	GGYRCC	3 cut(s)	182, 752, 1490
BsiEI	CGRYCG	1 cut(s)	2397
BsiHKAI	GWGCWC	1 cut(s)	239
BsiHKCI	CYCGRG	2 cut(s)	75, 3240
BsiSI	CCGG	8 cut(s)	263, 296, 882, 1764, 2098, 2393, 3425, 3609
BslFI	GGGAC	6 cut(s)	159, 491, 1521, 2476, 2895, 4270
BslI	CCNNNNNNNGG	8 cut(s)	143, 1313, 1593, 1658, 2837, 3384, 3797, 4159
BsmAI	GTCTC	5 cut(s)	1713, 1751, 1988, 4062, 4336
BsmFI	GGGAC	6 cut(s)	159, 491, 1521, 2476, 2895, 4270
BsmI	GAATGC	3 cut(s)	2224, 2405, 4228
BsnI	GGCC	4 cut(s)	1080, 1213, 2646, 4203
BsoBI	CYCGRG	2 cut(s)	75, 3240
Bsp119I	TTCGAA	1 cut(s)	937
Bsp1286I	GDGCHC	1 cut(s)	239
Bsp1407I	TGTACA	1 cut(s)	3969
Bsp1720I	GCTNAGC	2 cut(s)	2787, 3878
BspACI	CCGC	6 cut(s)	756, 991, 2450, 2643, 3057, 3569
BspANI	GGCC	4 cut(s)	1080, 1213, 2646, 4203
BspCNI	CTCAG	8 cut(s)	963, 1012, 1458, 1807, 1859, 2806, 3870, 4332
BspFNI	CGCG	1 cut(s)	1862
BspHI	TCATGA	2 cut(s)	2913, 3868
BspLI	GGNNCC	6 cut(s)	184, 220, 754, 1429, 1492, 2543
BspMAI	CTGCAG	3 cut(s)	356, 2593, 3128
BspMI	ACCTGC	1 cut(s)	1636
BspQI	GCTCTTC	1 cut(s)	2314
BspT104I	TTCGAA	1 cut(s)	937
BspT107I	GGYRCC	3 cut(s)	182, 752, 1490
BspTI	CTTAAG	1 cut(s)	4018
BsrBI	CCGCTC	1 cut(s)	3059
BsrDI	GCAATG	3 cut(s)	1681, 1756, 3639
BsrFI	RCCGGY	1 cut(s)	3608
BsrGI	TGTACA	1 cut(s)	3969
BssAI	RCCGGY	1 cut(s)	3608
BssSI	CACGAG	2 cut(s)	774, 2419
BssT1I	CCWWGG	5 cut(s)	1075, 2212, 2831, 2960, 3795
Bst2BI	CACGAG	2 cut(s)	774, 2419
Bst2UI	CCWGG	7 cut(s)	125, 551, 2460, 3339, 3705, 3755, 4040
Bst6I	CTCTTC	4 cut(s)	526, 1986, 2314, 3992
BstAFI	CTTAAG	1 cut(s)	4018
BstAPI	GCANNNNNTGC	5 cut(s)	360, 2666, 2696, 3041, 3577
BstAUI	TGTACA	1 cut(s)	3969
BstBAI	YACGTR	1 cut(s)	2297
BstBI	TTCGAA	1 cut(s)	937
BstC8I	GCNNGC	8 cut(s)	43, 1067, 1468, 1664, 2151, 3687, 3691, 4205
BstDSI	CCRYGG	1 cut(s)	4354
BstF5I	GGATG	6 cut(s)	247, 878, 2107, 2351, 3457, 4258
BstFNI	CGCG	1 cut(s)	1862
BstH2I	RGCGCY	1 cut(s)	2858
BstHHI	GCGC	1 cut(s)	2857
BstMAI	GTCTC	5 cut(s)	1713, 1751, 1988, 4062, 4336
BstMCI	CGRYCG	1 cut(s)	2397
BstNI	CCWGG	7 cut(s)	125, 551, 2460, 3339, 3705, 3755, 4040
BstNSI	RCATGY	7 cut(s)	303, 1106, 1621, 1875, 1935, 3662, 3968
BstPAI	GACNNNNGTC	1 cut(s)	3417
BstSFI	CTRYAG	4 cut(s)	352, 1275, 2589, 3124
BstSLI	GKGCMC	1 cut(s)	239
BstUI	CGCG	1 cut(s)	1862
BstV2I	GAAGAC	4 cut(s)	2833, 3210, 3413, 3840
BstX2I	RGATCY	6 cut(s)	335, 493, 1014, 1257, 1570, 3839
BstXI	CCANNNNNNTGG	1 cut(s)	2654
BstYI	RGATCY	6 cut(s)	335, 493, 1014, 1257, 1570, 3839
BsuI	GTATCC	2 cut(s)	1357, 1861
BsuRI	GGCC	4 cut(s)	1080, 1213, 2646, 4203
BtgI	CCRYGG	1 cut(s)	4354
BtsCI	GGATG	6 cut(s)	247, 878, 2107, 2351, 3457, 4258
BtsI	GCAGTG	1 cut(s)	3483
BveI	ACCTGC	1 cut(s)	1636
Cac8I	GCNNGC	8 cut(s)	43, 1067, 1468, 1664, 2151, 3687, 3691, 4205
CaiI	CAGNNNCTG	1 cut(s)	1774
CciI	TCATGA	2 cut(s)	2913, 3868
CfoI	GCGC	1 cut(s)	2857
Cfr10I	RCCGGY	1 cut(s)	3608
Cfr13I	GGNCC	3 cut(s)	219, 3707, 4358
CseI	GACGC	2 cut(s)	1851, 3776
CsiI	ACCWGGT	1 cut(s)	3337
Csp6I	GTAC	8 cut(s)	56, 183, 432, 1935, 2996, 3970, 4244, 4276
CviQI	GTAC	8 cut(s)	56, 183, 432, 1935, 2996, 3970, 4244, 4276
DraI	TTTAAA	1 cut(s)	282
DraIII	CACNNNGTG	1 cut(s)	3341
EaeI	YGGCCR	3 cut(s)	1078, 2644, 4201
Eam1104I	CTCTTC	4 cut(s)	526, 1986, 2314, 3992
EarI	CTCTTC	4 cut(s)	526, 1986, 2314, 3992
Eco130I	CCWWGG	5 cut(s)	1075, 2212, 2831, 2960, 3795
Eco147I	AGGCCT	1 cut(s)	1213
Eco32I	GATATC	1 cut(s)	4306
Eco47I	GGWCC	3 cut(s)	219, 3707, 4358
Eco47III	AGCGCT	1 cut(s)	2856
Eco57I	CTGAAG	6 cut(s)	1719, 2010, 2703, 2768, 3210, 3243
Eco72I	CACGTG	1 cut(s)	2297
Eco88I	CYCGRG	2 cut(s)	75, 3240
EcoRII	CCWGG	7 cut(s)	123, 549, 2458, 3337, 3703, 3753, 4038
EcoRV	GATATC	1 cut(s)	4306
EcoT14I	CCWWGG	5 cut(s)	1075, 2212, 2831, 2960, 3795
EcoT22I	ATGCAT	4 cut(s)	1648, 2278, 2952, 3664
ErhI	CCWWGG	5 cut(s)	1075, 2212, 2831, 2960, 3795
FalI	AAGNNNNNCTT	4 cut(s)	258, 290, 4012, 4044
FaqI	GGGAC	6 cut(s)	159, 491, 1521, 2476, 2895, 4270
FauI	CCCGC	1 cut(s)	998
FauNDI	CATATG	2 cut(s)	2536, 2614
FbaI	TGATCA	2 cut(s)	925, 2284
FblI	GTMKAC	1 cut(s)	2719
FokI	GGATG	6 cut(s)	234, 865, 2114, 2338, 3444, 4265
GlaI	GCGC	1 cut(s)	2856
GsaI	CCCAGC	2 cut(s)	770, 3834
GsuI	CTGGAG	5 cut(s)	1172, 3045, 3311, 3737, 3756
HaeII	RGCGCY	1 cut(s)	2858
HaeIII	GGCC	4 cut(s)	1080, 1213, 2646, 4203
HapII	CCGG	8 cut(s)	263, 296, 882, 1764, 2098, 2393, 3425, 3609
HgaI	GACGC	2 cut(s)	1851, 3776
HhaI	GCGC	1 cut(s)	2857
Hin6I	GCGC	1 cut(s)	2855
HinP1I	GCGC	1 cut(s)	2855
HindIII	AAGCTT	2 cut(s)	2819, 3687
HpaII	CCGG	8 cut(s)	263, 296, 882, 1764, 2098, 2393, 3425, 3609
HphI	GGTGA	9 cut(s)	965, 1106, 1328, 1340, 1545, 3262, 3327, 3353, 4142
Hpy166II	GTNNAC	7 cut(s)	237, 1270, 2720, 3335, 3344, 3809, 3970
Hpy8I	GTNNAC	7 cut(s)	237, 1270, 2720, 3335, 3344, 3809, 3970
Hpy99I	CGWCG	2 cut(s)	1867, 3473
HpyAV	CCTTC	9 cut(s)	1271, 1346, 1445, 1584, 1662, 1694, 2084, 3000, 3375
HpyCH4IV	ACGT	2 cut(s)	2296, 3468
HpySE526I	ACGT	2 cut(s)	2296, 3468
HspAI	GCGC	1 cut(s)	2855
KpnI	GGTACC	1 cut(s)	186
Ksp22I	TGATCA	2 cut(s)	925, 2284
LguI	GCTCTTC	1 cut(s)	2314
LmnI	GCTCC	5 cut(s)	2852, 3064, 3934, 3964, 4318
MabI	ACCWGGT	1 cut(s)	3337
MaeII	ACGT	2 cut(s)	2296, 3468
MbiI	CCGCTC	1 cut(s)	3059
MfeI	CAATTG	1 cut(s)	3650
MflI	RGATCY	6 cut(s)	335, 493, 1014, 1257, 1570, 3839
MhlI	GDGCHC	1 cut(s)	239
MlsI	TGGCCA	2 cut(s)	1080, 4203
MluNI	TGGCCA	2 cut(s)	1080, 4203
MlyI	GAGTC	7 cut(s)	1380, 2348, 2431, 2458, 2877, 3252, 3542
MmeI	TCCRAC	8 cut(s)	182, 433, 629, 1455, 1845, 2296, 3510, 3921
Mox20I	TGGCCA	2 cut(s)	1080, 4203
Mph1103I	ATGCAT	4 cut(s)	1648, 2278, 2952, 3664
MroXI	GAANNNNTTC	3 cut(s)	1736, 3331, 3515
MscI	TGGCCA	2 cut(s)	1080, 4203
MslI	CAYNNNNRTG	8 cut(s)	63, 1344, 1496, 2918, 3775, 4086, 4221, 4251
Msp20I	TGGCCA	2 cut(s)	1080, 4203
MspA1I	CMGCKG	2 cut(s)	119, 2548
MspCI	CTTAAG	1 cut(s)	4018
MspI	CCGG	8 cut(s)	263, 296, 882, 1764, 2098, 2393, 3425, 3609
MunI	CAATTG	1 cut(s)	3650
Mva1269I	GAATGC	3 cut(s)	2224, 2405, 4228
MvaI	CCWGG	7 cut(s)	125, 551, 2460, 3339, 3705, 3755, 4040
MvnI	CGCG	1 cut(s)	1862
NciI	CCSGG	3 cut(s)	296, 1764, 2099
NdeI	CATATG	2 cut(s)	2536, 2614
NlaIV	GGNNCC	6 cut(s)	184, 220, 754, 1429, 1492, 2543
NmuCI	GTSAC	8 cut(s)	640, 661, 1094, 2068, 2416, 3157, 3447, 3667
NsiI	ATGCAT	4 cut(s)	1648, 2278, 2952, 3664
NspI	RCATGY	7 cut(s)	303, 1106, 1621, 1875, 1935, 3662, 3968
NspV	TTCGAA	1 cut(s)	937
PaeR7I	CTCGAG	1 cut(s)	3240
PagI	TCATGA	2 cut(s)	2913, 3868
PceI	AGGCCT	1 cut(s)	1213
PciI	ACATGT	3 cut(s)	1102, 1871, 3964
PciSI	GCTCTTC	1 cut(s)	2314
PcsI	WCGNNNNNNNCGW	1 cut(s)	4256
PctI	GAATGC	3 cut(s)	2224, 2405, 4228
PdmI	GAANNNNTTC	3 cut(s)	1736, 3331, 3515
PfoI	TCCNGGA	2 cut(s)	2097, 4038
PleI	GAGTC	7 cut(s)	1379, 2347, 2430, 2458, 2877, 3251, 3541
PmaCI	CACGTG	1 cut(s)	2297
PmlI	CACGTG	1 cut(s)	2297
PpsI	GAGTC	7 cut(s)	1379, 2347, 2430, 2458, 2877, 3251, 3541
Ppu21I	YACGTR	1 cut(s)	2297
PscI	ACATGT	3 cut(s)	1102, 1871, 3964
PshAI	GACNNNNGTC	1 cut(s)	3417
PshBI	ATTAAT	1 cut(s)	107
Psp6I	CCWGG	7 cut(s)	123, 549, 2458, 3337, 3703, 3753, 4038
PspCI	CACGTG	1 cut(s)	2297
PspFI	CCCAGC	2 cut(s)	766, 3830
PspGI	CCWGG	7 cut(s)	123, 549, 2458, 3337, 3703, 3753, 4038
PspN4I	GGNNCC	6 cut(s)	184, 220, 754, 1429, 1492, 2543
PspPI	GGNCC	3 cut(s)	219, 3707, 4358
PspXI	VCTCGAGB	1 cut(s)	3240
PstI	CTGCAG	3 cut(s)	356, 2593, 3128
PstNI	CAGNNNCTG	1 cut(s)	1774
PsuI	RGATCY	6 cut(s)	335, 493, 1014, 1257, 1570, 3839
PvuII	CAGCTG	2 cut(s)	119, 2548
RsaI	GTAC	8 cut(s)	57, 184, 433, 1936, 2997, 3971, 4245, 4277
RsaNI	GTAC	8 cut(s)	56, 183, 432, 1935, 2996, 3970, 4244, 4276
RseI	CAYNNNNRTG	8 cut(s)	63, 1344, 1496, 2918, 3775, 4086, 4221, 4251
SapI	GCTCTTC	1 cut(s)	2314
Sau96I	GGNCC	3 cut(s)	219, 3707, 4358
SchI	GAGTC	7 cut(s)	1380, 2348, 2431, 2458, 2877, 3252, 3542
SduI	GDGCHC	1 cut(s)	239
SexAI	ACCWGGT	1 cut(s)	3337
SfcI	CTRYAG	4 cut(s)	352, 1275, 2589, 3124
Sfr274I	CTCGAG	1 cut(s)	3240
SfuI	TTCGAA	1 cut(s)	937
SgrAI	CRCCGGYG	1 cut(s)	3608
SinI	GGWCC	3 cut(s)	219, 3707, 4358
SlaI	CTCGAG	1 cut(s)	3240
SmiMI	CAYNNNNRTG	8 cut(s)	63, 1344, 1496, 2918, 3775, 4086, 4221, 4251
SmlI	CTYRAG	5 cut(s)	886, 1831, 3240, 3352, 4018
SmoI	CTYRAG	5 cut(s)	886, 1831, 3240, 3352, 4018
SpeI	ACTAGT	3 cut(s)	28, 736, 2230
SseBI	AGGCCT	1 cut(s)	1213
SsiI	CCGC	6 cut(s)	756, 991, 2450, 2643, 3057, 3569
StuI	AGGCCT	1 cut(s)	1213
StyI	CCWWGG	5 cut(s)	1075, 2212, 2831, 2960, 3795
TaiI	ACGT	2 cut(s)	2299, 3471
TatI	WGTACW	4 cut(s)	431, 1934, 3969, 4243
TauI	GCSGC	1 cut(s)	2646
TseFI	GTSAC	8 cut(s)	640, 661, 1094, 2068, 2416, 3157, 3447, 3667
Tsp45I	GTSAC	8 cut(s)	640, 661, 1094, 2068, 2416, 3157, 3447, 3667
TspGWI	ACGGA	8 cut(s)	101, 222, 358, 858, 1576, 2532, 3780, 4265
Vha464I	CTTAAG	1 cut(s)	4018
VneI	GTGCAC	1 cut(s)	235
VpaK11BI	GGWCC	3 cut(s)	219, 3707, 4358
VspI	ATTAAT	1 cut(s)	107
XapI	RAATTY	6 cut(s)	795, 1633, 1970, 2110, 3088, 4145
XbaI	TCTAGA	2 cut(s)	410, 2875
XceI	RCATGY	7 cut(s)	303, 1106, 1621, 1875, 1935, 3662, 3968
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1073
XhoI	CTCGAG	1 cut(s)	3240
XmaJI	CCTAGG	1 cut(s)	2831
XmiI	GTMKAC	1 cut(s)	2719
XmnI	GAANNNNTTC	3 cut(s)	1736, 3331, 3515
Zsp2I	ATGCAT	4 cut(s)	1648, 2278, 2952, 3664

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.