Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0002992

Physical Location & Seq

Genomic Coordinates Reverse (-)

51048145 .. 51060394

Gene Structure 12250 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00256700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3963 bp

ATGGAAGATGAATTGTATAATGAAGAACCAGTGCCATGTGCTTGCATAACTGGGGTACATCTAAATGTTTCTACTCGGGAAGATACGGAGAAAATATCAACATTATTAATCGAGGCAGCTGCCCAGGTTAATGATCCAAAGTTGGGACTTCCAAATCCAACGAATCAGTGTTCTACTTGTGGTACCCAAGATATAAAATCCTGTGATGGTCATTTTGGGTCCATCAAGTTTCCATGCACGATACTCCATCCATACTATCTACCGGAAGTTGCTCTTATTTTAAACAAGATTTGCCCGGCATGTAAAACCATCAGGCAAGGTGTAAGGGTCAAGGGCTCTGAACGGAGGGGCTGCAGATATTGCTTTGGAGGTTCATCGGATTACCCTAGAATGAAGTTCAAAGTTTCTTCTAGAGAGCTTTTCAGAAGAAGTACAATAATAGTAGAAGCAGTTGGAGAATTGCCTCCTGATTATTGGGACTTCATTCCCAAAGATCCCCAACATGATGAAACACACACAAAACCAAATAGAAGAGTTCTATCTCATGCCCAGGTTCATTTTTTGTTGAATGATGTTGATCCAGATTTCATCACAAAGTTTGTTCCAACAACCAGCTCACTTTCTTTGGACTGTTTCCTTGTCACGCCAAATTGTCATCGTGTGACAGAAGTTATGTATTCATTTAATAATGGGCAGTCATTGATGTTTGATGATCGGACTAGAGCTTACAGGAAACTTGTGGATTTCAGAGGCACTGCGAACGAGCTGGGTTCTCGTGTTTTGGACTGCCTAAAAATTTCCAAGATAAACAAAGACAAAACAGGCAGAGATTCAATTTCAGTTGAGCAACAAAAGATTAAGGATTCTCCGTCCAGAACATCTGGCTTGAGATGGATAAAAGATGTTGTTCTTGGAAAGAGAAGTGATCACTGTTTTCGAACTGTCGTCGTTGGTGATCCAAACATCAAGCTGAGTGAGATTGGCATACCCCGCCATATTGCAGAGAAAATGCAGATCTCTGAGAATCTGAACCAATATAACCCTGAGAAGTTGTATGCGTTTTGCCAGCTACGCCTTGGCCATCGGGGGAGAGGTGACATACATGTTCGTAGAAATGATAGTCTGGTTAGGATAAGTAATTTAGAAGAACTGGAGATGGGAGATATTATATACAGGGCGTTGAGTGATGGAGATGTTGTGTTGATAAATAGGCCTCCATCAATACATCAACACTCCCTAATAGCTTTAACCGCAAAGGTCCTTCCAGTAAACTCTGTAGTGTCGATAAATCCACTGTGTTGCTCTCCTTTTCGTGGTGATTTTGATGGTGATTGCCTTCATGGTTATGTTCCTCAATCAGTGGATACCAGAGTCGAGCTTACAGAGCTTGTTGCTTTAGATAAGCAATTAGTTAATGGGCAGAGTGGTTCCAACCTTCTTTCTCTCAGTCAAGATAGTTTAACTGCTTGCTATCTCATCATGGAAGATGGCACCCTGATGAATAAGTTTCAAATGCAACAACTGAAAATGCTTTGTCCCCTTGAACTGCCATCACCAGCAATAATTAAAGATCCTTCTGGTAACTCCGTTTCTTGGACGGCAAAGCAGATAATCAGCATGTTTCTACCTGCAGATTTTAATTATGCTTTTCCTTCAAATGGCGTGCATATTAGCAATGGAGAGCTTCTAGCTTTTTCTGAAGGTTCTTCTTGGCTGCGAGACACCAGTGGGAATCTTTTCCAAAGTCTCATCGAACATTGCCCCGGTCAGATACTTGACATCTTCTTTACTGCTCAGGGAGTTCTATGTGAGTGGTTGTCAATGAGGGGCTTGAGTGTTTCTCTCTCAGACTTGTATCTCGCGTCGGATACATGTTCAAGGAAAAACTTGGTGGAGGAAATCTTCTATGGGTTACAAGAAGCAGAGGAGGCATGTACTATCAGACAGTTGATGCTTGATTCTTGTCAGGAATTTCTTATGGGCTATGCTGAAGAGACAAAAAGTCCTTTGACTTTTGGTTCAGATCATTTTTGTTATCAGAAGCAGAAATCTGCTGCACTTAGTCAGGTCACGGTAGATGCTTTCAAAGAAGGTTTCCGGGATGTCCAAAATTTAGCATACAAATATGCCCGTGAGGACAACTCGTTGCTGGCTATGTTGAAGGCTGGAAGCAAGGGTAATATGCTGAAATTAGTTCAGCATGGTATGTGCCTTGGTTTGCAGCATTCACTGGTTCCCTTATCATTCAAGTTTCCGAACCGGCTCTCATGTGATGCATGGAATGATCAAAAAGCACATGGTATCGTTCAGGAGGTTGGAAGAGCTTTTGAATCCATCGAGTCATATATCCCATCCGCTGTTGTTAAAAGTTCGTTCTTGACTGGGCTGAATCCGGTCGAATGCTTTGTTCATTCAGTCACGAGTCGAAATGGTTCTTTTAGTAATAATGCGGAACTTCCTGGGACTCTGAATCGAAAGCTCATGTATTTCATGCGTGATTTGCATACTGCTTATGATGGAACAGTTAGAAACGCATATGGGAACCAGCTGGTTCAGTTCTCCTATAATATTGACGAGGGCATATCTACTGCAGATAGCACCACAAACAGCTCATATGCAAAGAGTGTTATTGATTGTGGTGGCGGCCAACCCGTTGGTGCATTGTCTGCTTGTGCAATATCTGAAGCTGCATACAGTGCTTTAGATCAGCCAGTTAGTCTACTTGAAACTTCTCCTCTGCTAAATCTGAAGAACATTTTGGAGTGTGGTTCAAAGAAAAGCAGTGCTAAGCAAACTATGTCATTATTTTTATCTGAGAAGCTTGGAAGACATAGGCATGGTTTCGAGTATGGAGCGCTAGAAGTTAAAAATCATCTAGAGGGACTCACATTTTCAGAAATCGTTTCTACTGTCATGATAATGTTCTCCTCACAAACTGGCAGCAAGATGCATTTTAGTCCTTGGGTTTCTCACTTTCATATATCTAAGGAAATTGTACGAAGAAGAAGGCTGAATGTGCGATCTATTATAGAGGCACTTTATGCAAGATGCAACTCCGCTGGAGCGGAGTTGAAAATTATCCTTTGGAATTTGAAGATCACAAGAAATAAGGAATGTGACTGCTGCAGCACACCTAATGATACATTTTGCATCAGTGTCACACTGACTGAAAAGCACGACAATTCTTCTGAAGAACTGGATAAAACTCGAGCCTTGGTGATACCATTCTTGCTTAAAACAGTAGTTAAAGGTTTTCTGGAGATAAAGAAGGTGGATATTATATGGAATGATCGACCTGGTTCATCTGGTGAACTCTGCTTGAGAGTTTCTATGTCACGAAAGACCAGAAGGGGGACTACCTTTTGGAATATGCTTGTGGACGATTGTCTTCCGGTTATGGATATGATTGATTGGTCACGAAGTCATCCAGATAACGTCGATGACTTCTGCACTGCCTATGGAATAGATGTTGGGTGGAAACATTTCCTTAATAAGTTGGAGTCTGCAACCGTAGATATTGGAAAGACAATTCTTCCTCAGCATTTGCTTCTTGCAGCAGATTGTCTGTCAGCCACCGGCGAGTTTGTCAGCTTAAACGCAAAAGGCATTGCTCAACAATTGGAACATGCATCTGTCACAGCCCCTTTTATGCAAGCTTGCTTTTCTACTCCTGGTCCTTGCTTGGTTAAAGCTGCTAAGGCTGGAGTGGTGGATAATCTCCAGGGGAGTTTGGACGCATTGACATGGGGAAATACTCCGTCCTTGGGAAGTGGTGGACAGTTTGATATTATATTTGCTGTGGAGGGATCTGTGTCTTCTAAGCCATTTGATGTTCATGATATGCTGAGCAGACAAATCAGTTCCAACCCCAGGATGTATTTGAAATGTCTGATGCTCCAATTCATCTATCTGACAAATATGTTGCTCCACATGTGTACAAGTATGGTCTTTTGTGCAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000418 GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010495 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901698 GO:1901699 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1320

Amino Acids

146.83

Weight (kDa)

6.43

Isoelectric Point (pI)

44.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	43 - 104	3.9e-07	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	305 - 464	8.1e-35	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	469 - 618	8.8e-20	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	687 - 742	2.9e-08	RNA polymerase Rpb1, domain 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002992)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63020 AT1G63020 AT1G63020 AT1G63020 AT1G63020
fragaria_vesca	FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400
malus_domestica	MD04G1068800.v1.1 MD06G1069300.v1.1 MD06G1069400.v1.1 MD06G1069500.v1.1 MD06G1069800.v1.1
prunus_persica	Prupe.5G089300_v2.0.a1
pyrus_communis	pycom04g06250
rosa_chinensis	RchiOBHm_Chr7g0199481
rosa_laevigata	RLG00000003790
rosa_multiflora	Rmu_co8411067.1_g000001 Rmu_sc0003890.1_g000001
rosa_roxburghii	Rroxscaffold_3G00256700
rosa_rugosa	Rorug07G0054300 Rorug07G0054400 Rorug07G0054500 Rorug07G0054500
rosa_samantha	Rh7AG179300 Rh7BG182800 Rh7CG188100 Rh7DG182200
rosa_wichuraiana	Rw7G015760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1636
Acc65I	GGTACC	1 cut(s)	182
AccB1I	GGYRCC	2 cut(s)	182, 1490
AccBSI	CCGCTC	1 cut(s)	3065
AccI	GTMKAC	1 cut(s)	2719
AccII	CGCG	1 cut(s)	1862
AciI	CCGC	7 cut(s)	991, 1251, 2355, 2450, 2643, 3057, 3065
AclWI	GGATC	6 cut(s)	128, 488, 572, 950, 1565, 3814
AcoI	YGGCCR	2 cut(s)	1078, 2644
AcsI	RAATTY	4 cut(s)	795, 1970, 2110, 3088
AcuI	CTGAAG	5 cut(s)	1719, 2010, 2703, 2768, 3210
AfaI	GTAC	6 cut(s)	57, 184, 433, 1936, 2997, 3937
AfeI	AGCGCT	1 cut(s)	2856
AfiI	CCNNNNNNNGG	7 cut(s)	143, 1313, 1593, 1658, 3351, 3764, 3870
AflIII	ACRYGT	3 cut(s)	1102, 1871, 3930
AhdI	GACNNNNNGTC	1 cut(s)	2001
AjnI	CCWGG	7 cut(s)	123, 549, 2458, 3295, 3670, 3720, 3869
AjuI	GAANNNNNNNTTGG	2 cut(s)	2741, 2773
Alw26I	GTCTC	3 cut(s)	1713, 1751, 1988
AlwI	GGATC	6 cut(s)	128, 488, 572, 950, 1565, 3814
Ama87I	CYCGRG	2 cut(s)	75, 3207
Aor51HI	AGCGCT	1 cut(s)	2856
AoxI	GGCC	3 cut(s)	1078, 1211, 2644
ApoI	RAATTY	4 cut(s)	795, 1970, 2110, 3088
AseI	ATTAAT	1 cut(s)	107
Asp700I	GAANNNNTTC	3 cut(s)	1736, 2431, 3482
Asp718I	GGTACC	1 cut(s)	182
AspLEI	GCGC	1 cut(s)	2857
AspS9I	GGNCC	3 cut(s)	219, 1258, 3674
AsuC2I	CCSGG	3 cut(s)	296, 1764, 2099
AsuHPI	GGTGA	7 cut(s)	965, 1106, 1328, 1340, 1545, 3229, 3320
AsuII	TTCGAA	1 cut(s)	937
AvaI	CYCGRG	2 cut(s)	75, 3207
AvaII	GGWCC	3 cut(s)	219, 1258, 3674
BaeI	ACNNNNGTAYC	2 cut(s)	1356, 1389
BalI	TGGCCA	1 cut(s)	1080
BanI	GGYRCC	2 cut(s)	182, 1490
BanII	GRGCYC	1 cut(s)	338
BarI	GAAGNNNNNNTAC	2 cut(s)	3340, 3372
BauI	CACGAG	2 cut(s)	774, 2419
BbsI	GAAGAC	3 cut(s)	2833, 3380, 3807
BbvCI	CCTCAGC	1 cut(s)	3537
BceAI	ACGGC	1 cut(s)	1614
BcgI	CGANNNNNNTGC	2 cut(s)	101, 135
BciT130I	CCWGG	7 cut(s)	125, 551, 2460, 3297, 3672, 3722, 3871
BciVI	GTATCC	2 cut(s)	1357, 1861
BclI	TGATCA	2 cut(s)	925, 2284
BcnI	CCSGG	3 cut(s)	296, 1764, 2099
BcoDI	GTCTC	3 cut(s)	1713, 1751, 1988
BfaI	CTAG	6 cut(s)	387, 411, 720, 1688, 2858, 2876
BfmI	CTRYAG	5 cut(s)	352, 1275, 1629, 2589, 3124
BfoI	RGCGCY	1 cut(s)	2858
BfuAI	ACCTGC	1 cut(s)	1636
BfuI	GTATCC	2 cut(s)	1357, 1861
BglII	AGATCT	1 cut(s)	1014
BlpI	GCTNAGC	2 cut(s)	2787, 3845
Bme18I	GGWCC	3 cut(s)	219, 1258, 3674
BmeRI	GACNNNNNGTC	1 cut(s)	2001
BmeT110I	CYCGRG	2 cut(s)	75, 3207
BmgT120I	GGNCC	3 cut(s)	219, 1258, 3674
BmiI	GGNNCC	6 cut(s)	184, 220, 1429, 1492, 2235, 2543
BmrI	ACTGGG	2 cut(s)	60, 2391
BmsI	GCATC	8 cut(s)	1941, 2068, 2263, 2937, 3038, 3159, 3638, 3882
BmuI	ACTGGG	2 cut(s)	60, 2391
BoxI	GACNNNNGTC	1 cut(s)	3384
BpiI	GAAGAC	3 cut(s)	2833, 3380, 3807
BplI	GAGNNNNNCTC	4 cut(s)	1825, 1857, 2126, 2158
BpmI	CTGGAG	5 cut(s)	1172, 3081, 3278, 3704, 3723
Bpu10I	CCTNAGC	3 cut(s)	1794, 3537, 3696
Bpu1102I	GCTNAGC	2 cut(s)	2787, 3845
Bpu14I	TTCGAA	1 cut(s)	937
BpuEI	CTTGAG	3 cut(s)	907, 1852, 3340
BpuMI	CCSGG	3 cut(s)	296, 1764, 2099
BsaBI	GATNNNNATC	1 cut(s)	576
BsaWI	WCCGGW	3 cut(s)	262, 2392, 3391
BsaXI	ACNNNNNCTCC	2 cut(s)	1182, 1212
Bsc4I	CCNNNNNNNGG	7 cut(s)	143, 1313, 1593, 1658, 3351, 3764, 3870
Bse118I	RCCGGY	2 cut(s)	2259, 3575
Bse3DI	GCAATG	3 cut(s)	1681, 1756, 3606
Bse8I	GATNNNNATC	1 cut(s)	576
BseBI	CCWGG	7 cut(s)	125, 551, 2460, 3297, 3672, 3722, 3871
BseGI	GGATG	5 cut(s)	247, 2107, 2351, 3424, 3879
BseJI	GATNNNNATC	1 cut(s)	576
BseLI	CCNNNNNNNGG	7 cut(s)	143, 1313, 1593, 1658, 3351, 3764, 3870
BseMI	GCAATG	3 cut(s)	1681, 1756, 3606
BseMII	CTCAG	9 cut(s)	962, 1011, 1035, 1459, 1808, 1860, 2805, 3551, 3836
BseRI	GAGGAG	3 cut(s)	1940, 2724, 2917
BseYI	CCCAGC	1 cut(s)	766
BsgI	GTGCAG	2 cut(s)	2040, 3433
Bsh1236I	CGCG	1 cut(s)	1862
Bsh1285I	CGRYCG	1 cut(s)	2397
BshFI	GGCC	3 cut(s)	1080, 1213, 2646
BshNI	GGYRCC	2 cut(s)	182, 1490
BsiEI	CGRYCG	1 cut(s)	2397
BsiHKCI	CYCGRG	2 cut(s)	75, 3207
BsiSI	CCGG	8 cut(s)	263, 296, 1764, 2098, 2260, 2393, 3392, 3576
BslFI	GGGAC	6 cut(s)	159, 491, 1521, 2476, 2895, 3367
BslI	CCNNNNNNNGG	7 cut(s)	143, 1313, 1593, 1658, 3351, 3764, 3870
BsmAI	GTCTC	3 cut(s)	1713, 1751, 1988
BsmFI	GGGAC	6 cut(s)	159, 491, 1521, 2476, 2895, 3367
BsmI	GAATGC	2 cut(s)	2224, 2405
BsnI	GGCC	3 cut(s)	1080, 1213, 2646
BsoBI	CYCGRG	2 cut(s)	75, 3207
Bsp119I	TTCGAA	1 cut(s)	937
Bsp1286I	GDGCHC	1 cut(s)	338
Bsp1407I	TGTACA	1 cut(s)	3935
Bsp1720I	GCTNAGC	2 cut(s)	2787, 3845
BspACI	CCGC	7 cut(s)	991, 1251, 2355, 2450, 2643, 3057, 3065
BspANI	GGCC	3 cut(s)	1080, 1213, 2646
BspCNI	CTCAG	9 cut(s)	963, 1012, 1036, 1458, 1807, 1859, 2806, 3550, 3837
BspFNI	CGCG	1 cut(s)	1862
BspHI	TCATGA	2 cut(s)	2913, 3835
BspLI	GGNNCC	6 cut(s)	184, 220, 1429, 1492, 2235, 2543
BspMAI	CTGCAG	4 cut(s)	356, 1633, 2593, 3128
BspMI	ACCTGC	1 cut(s)	1636
BspPI	GGATC	6 cut(s)	128, 488, 572, 950, 1565, 3814
BspQI	GCTCTTC	1 cut(s)	2314
BspT104I	TTCGAA	1 cut(s)	937
BspT107I	GGYRCC	2 cut(s)	182, 1490
BsrBI	CCGCTC	1 cut(s)	3065
BsrDI	GCAATG	3 cut(s)	1681, 1756, 3606
BsrFI	RCCGGY	2 cut(s)	2259, 3575
BsrGI	TGTACA	1 cut(s)	3935
BssAI	RCCGGY	2 cut(s)	2259, 3575
BssSI	CACGAG	2 cut(s)	774, 2419
BssT1I	CCWWGG	5 cut(s)	1075, 2212, 2960, 3213, 3762
Bst2BI	CACGAG	2 cut(s)	774, 2419
Bst2UI	CCWGG	7 cut(s)	125, 551, 2460, 3297, 3672, 3722, 3871
Bst6I	CTCTTC	3 cut(s)	526, 1986, 2314
BstAPI	GCANNNNNTGC	4 cut(s)	360, 2666, 2696, 3041
BstAUI	TGTACA	1 cut(s)	3935
BstBI	TTCGAA	1 cut(s)	937
BstC8I	GCNNGC	7 cut(s)	43, 1067, 1468, 1664, 2151, 3654, 3658
BstF5I	GGATG	5 cut(s)	247, 2107, 2351, 3424, 3879
BstFNI	CGCG	1 cut(s)	1862
BstH2I	RGCGCY	1 cut(s)	2858
BstHHI	GCGC	1 cut(s)	2857
BstMAI	GTCTC	3 cut(s)	1713, 1751, 1988
BstMCI	CGRYCG	1 cut(s)	2397
BstNI	CCWGG	7 cut(s)	125, 551, 2460, 3297, 3672, 3722, 3871
BstNSI	RCATGY	7 cut(s)	303, 1106, 1621, 1875, 1935, 3629, 3934
BstPAI	GACNNNNGTC	1 cut(s)	3384
BstSFI	CTRYAG	5 cut(s)	352, 1275, 1629, 2589, 3124
BstUI	CGCG	1 cut(s)	1862
BstV2I	GAAGAC	3 cut(s)	2833, 3380, 3807
BstX2I	RGATCY	4 cut(s)	493, 1014, 1570, 3806
BstXI	CCANNNNNNTGG	1 cut(s)	2654
BstYI	RGATCY	4 cut(s)	493, 1014, 1570, 3806
BsuI	GTATCC	2 cut(s)	1357, 1861
BsuRI	GGCC	3 cut(s)	1080, 1213, 2646
BtsCI	GGATG	5 cut(s)	247, 2107, 2351, 3424, 3879
BtsI	GCAGTG	3 cut(s)	753, 2788, 3450
BveI	ACCTGC	1 cut(s)	1636
Cac8I	GCNNGC	7 cut(s)	43, 1067, 1468, 1664, 2151, 3654, 3658
CciI	TCATGA	2 cut(s)	2913, 3835
CfoI	GCGC	1 cut(s)	2857
Cfr10I	RCCGGY	2 cut(s)	2259, 3575
Cfr13I	GGNCC	3 cut(s)	219, 1258, 3674
CseI	GACGC	2 cut(s)	1851, 3743
CsiI	ACCWGGT	1 cut(s)	3295
Csp6I	GTAC	6 cut(s)	56, 183, 432, 1935, 2996, 3936
CviQI	GTAC	6 cut(s)	56, 183, 432, 1935, 2996, 3936
DraI	TTTAAA	1 cut(s)	282
DriI	GACNNNNNGTC	1 cut(s)	2001
EaeI	YGGCCR	2 cut(s)	1078, 2644
Eam1104I	CTCTTC	3 cut(s)	526, 1986, 2314
Eam1105I	GACNNNNNGTC	1 cut(s)	2001
EarI	CTCTTC	3 cut(s)	526, 1986, 2314
Eco130I	CCWWGG	5 cut(s)	1075, 2212, 2960, 3213, 3762
Eco147I	AGGCCT	1 cut(s)	1213
Eco24I	GRGCYC	1 cut(s)	338
Eco47I	GGWCC	3 cut(s)	219, 1258, 3674
Eco47III	AGCGCT	1 cut(s)	2856
Eco57I	CTGAAG	5 cut(s)	1719, 2010, 2703, 2768, 3210
Eco88I	CYCGRG	2 cut(s)	75, 3207
EcoO109I	RGGNCCY	1 cut(s)	1258
EcoRII	CCWGG	7 cut(s)	123, 549, 2458, 3295, 3670, 3720, 3869
EcoT14I	CCWWGG	5 cut(s)	1075, 2212, 2960, 3213, 3762
EcoT22I	ATGCAT	3 cut(s)	2278, 2952, 3631
EcoT38I	GRGCYC	1 cut(s)	338
ErhI	CCWWGG	5 cut(s)	1075, 2212, 2960, 3213, 3762
FalI	AAGNNNNNCTT	2 cut(s)	258, 290
FaqI	GGGAC	6 cut(s)	159, 491, 1521, 2476, 2895, 3367
FauI	CCCGC	1 cut(s)	998
FauNDI	CATATG	2 cut(s)	2536, 2614
FbaI	TGATCA	2 cut(s)	925, 2284
FblI	GTMKAC	1 cut(s)	2719
FokI	GGATG	5 cut(s)	234, 2114, 2338, 3411, 3886
FriOI	GRGCYC	1 cut(s)	338
FspBI	CTAG	6 cut(s)	387, 411, 720, 1688, 2858, 2876
GlaI	GCGC	1 cut(s)	2856
GsaI	CCCAGC	1 cut(s)	770
GsuI	CTGGAG	5 cut(s)	1172, 3081, 3278, 3704, 3723
HaeII	RGCGCY	1 cut(s)	2858
HaeIII	GGCC	3 cut(s)	1080, 1213, 2646
HapII	CCGG	8 cut(s)	263, 296, 1764, 2098, 2260, 2393, 3392, 3576
HgaI	GACGC	2 cut(s)	1851, 3743
HhaI	GCGC	1 cut(s)	2857
Hin6I	GCGC	1 cut(s)	2855
HinP1I	GCGC	1 cut(s)	2855
HindIII	AAGCTT	2 cut(s)	2819, 3654
HpaII	CCGG	8 cut(s)	263, 296, 1764, 2098, 2260, 2393, 3392, 3576
HphI	GGTGA	7 cut(s)	965, 1106, 1328, 1340, 1545, 3229, 3320
Hpy166II	GTNNAC	6 cut(s)	1270, 2720, 3311, 3379, 3776, 3936
Hpy8I	GTNNAC	6 cut(s)	1270, 2720, 3311, 3379, 3776, 3936
Hpy99I	CGWCG	3 cut(s)	950, 1867, 3440
HpyCH4IV	ACGT	1 cut(s)	3435
HpySE526I	ACGT	1 cut(s)	3435
HspAI	GCGC	1 cut(s)	2855
KpnI	GGTACC	1 cut(s)	186
Ksp22I	TGATCA	2 cut(s)	925, 2284
LguI	GCTCTTC	1 cut(s)	2314
LmnI	GCTCC	4 cut(s)	2852, 3062, 3900, 3930
LweI	GCATC	8 cut(s)	1941, 2068, 2263, 2937, 3038, 3159, 3638, 3882
MabI	ACCWGGT	1 cut(s)	3295
MaeI	CTAG	6 cut(s)	387, 411, 720, 1688, 2858, 2876
MaeII	ACGT	1 cut(s)	3435
MbiI	CCGCTC	1 cut(s)	3065
MfeI	CAATTG	1 cut(s)	3617
MflI	RGATCY	4 cut(s)	493, 1014, 1570, 3806
MhlI	GDGCHC	1 cut(s)	338
MlsI	TGGCCA	1 cut(s)	1080
MluNI	TGGCCA	1 cut(s)	1080
MlyI	GAGTC	6 cut(s)	1380, 2348, 2431, 2458, 2877, 3509
MmeI	TCCRAC	8 cut(s)	182, 433, 629, 1455, 1845, 2296, 3477, 3888
Mox20I	TGGCCA	1 cut(s)	1080
Mph1103I	ATGCAT	3 cut(s)	2278, 2952, 3631
MroXI	GAANNNNTTC	3 cut(s)	1736, 2431, 3482
MscI	TGGCCA	1 cut(s)	1080
MslI	CAYNNNNRTG	6 cut(s)	63, 1344, 1496, 2835, 2918, 3742
Msp20I	TGGCCA	1 cut(s)	1080
MspA1I	CMGCKG	4 cut(s)	119, 2357, 2548, 3059
MspI	CCGG	8 cut(s)	263, 296, 1764, 2098, 2260, 2393, 3392, 3576
MunI	CAATTG	1 cut(s)	3617
Mva1269I	GAATGC	2 cut(s)	2224, 2405
MvaI	CCWGG	7 cut(s)	125, 551, 2460, 3297, 3672, 3722, 3871
MvnI	CGCG	1 cut(s)	1862
NciI	CCSGG	3 cut(s)	296, 1764, 2099
NdeI	CATATG	2 cut(s)	2536, 2614
NlaIV	GGNNCC	6 cut(s)	184, 220, 1429, 1492, 2235, 2543
NsiI	ATGCAT	3 cut(s)	2278, 2952, 3631
NspI	RCATGY	7 cut(s)	303, 1106, 1621, 1875, 1935, 3629, 3934
NspV	TTCGAA	1 cut(s)	937
PaeR7I	CTCGAG	1 cut(s)	3207
PagI	TCATGA	2 cut(s)	2913, 3835
PceI	AGGCCT	1 cut(s)	1213
PciI	ACATGT	3 cut(s)	1102, 1871, 3930
PciSI	GCTCTTC	1 cut(s)	2314
PctI	GAATGC	2 cut(s)	2224, 2405
PdmI	GAANNNNTTC	3 cut(s)	1736, 2431, 3482
PfeI	GAWTC	9 cut(s)	163, 830, 863, 1024, 1732, 1958, 2330, 2389, 2470
PfoI	TCCNGGA	1 cut(s)	2097
PleI	GAGTC	6 cut(s)	1379, 2347, 2430, 2458, 2877, 3508
PpsI	GAGTC	6 cut(s)	1379, 2347, 2430, 2458, 2877, 3508
PpuMI	RGGWCCY	1 cut(s)	1258
PscI	ACATGT	3 cut(s)	1102, 1871, 3930
PshAI	GACNNNNGTC	1 cut(s)	3384
PshBI	ATTAAT	1 cut(s)	107
Psp5II	RGGWCCY	1 cut(s)	1258
Psp6I	CCWGG	7 cut(s)	123, 549, 2458, 3295, 3670, 3720, 3869
PspFI	CCCAGC	1 cut(s)	766
PspGI	CCWGG	7 cut(s)	123, 549, 2458, 3295, 3670, 3720, 3869
PspN4I	GGNNCC	6 cut(s)	184, 220, 1429, 1492, 2235, 2543
PspPI	GGNCC	3 cut(s)	219, 1258, 3674
PspPPI	RGGWCCY	1 cut(s)	1258
PspXI	VCTCGAGB	1 cut(s)	3207
PstI	CTGCAG	4 cut(s)	356, 1633, 2593, 3128
PsuI	RGATCY	4 cut(s)	493, 1014, 1570, 3806
PvuII	CAGCTG	2 cut(s)	119, 2548
RsaI	GTAC	6 cut(s)	57, 184, 433, 1936, 2997, 3937
RsaNI	GTAC	6 cut(s)	56, 183, 432, 1935, 2996, 3936
RseI	CAYNNNNRTG	6 cut(s)	63, 1344, 1496, 2835, 2918, 3742
SapI	GCTCTTC	1 cut(s)	2314
Sau96I	GGNCC	3 cut(s)	219, 1258, 3674
SchI	GAGTC	6 cut(s)	1380, 2348, 2431, 2458, 2877, 3509
SduI	GDGCHC	1 cut(s)	338
SexAI	ACCWGGT	1 cut(s)	3295
SfaNI	GCATC	8 cut(s)	1941, 2068, 2263, 2937, 3038, 3159, 3638, 3882
SfcI	CTRYAG	5 cut(s)	352, 1275, 1629, 2589, 3124
Sfr274I	CTCGAG	1 cut(s)	3207
SfuI	TTCGAA	1 cut(s)	937
SgrAI	CRCCGGYG	1 cut(s)	3575
SinI	GGWCC	3 cut(s)	219, 1258, 3674
SlaI	CTCGAG	1 cut(s)	3207
SmiMI	CAYNNNNRTG	6 cut(s)	63, 1344, 1496, 2835, 2918, 3742
SmlI	CTYRAG	4 cut(s)	886, 1831, 3207, 3319
SmoI	CTYRAG	4 cut(s)	886, 1831, 3207, 3319
SseBI	AGGCCT	1 cut(s)	1213
SsiI	CCGC	7 cut(s)	991, 1251, 2355, 2450, 2643, 3057, 3065
SspI	AATATT	1 cut(s)	2569
SspMI	CTAG	6 cut(s)	387, 411, 720, 1688, 2858, 2876
StuI	AGGCCT	1 cut(s)	1213
StyI	CCWWGG	5 cut(s)	1075, 2212, 2960, 3213, 3762
TaiI	ACGT	1 cut(s)	3438
TatI	WGTACW	3 cut(s)	431, 1934, 3935
TauI	GCSGC	1 cut(s)	2646
TfiI	GAWTC	9 cut(s)	163, 830, 863, 1024, 1732, 1958, 2330, 2389, 2470
TspGWI	ACGGA	5 cut(s)	101, 358, 858, 1576, 3747
VpaK11BI	GGWCC	3 cut(s)	219, 1258, 3674
VspI	ATTAAT	1 cut(s)	107
XapI	RAATTY	4 cut(s)	795, 1970, 2110, 3088
XbaI	TCTAGA	2 cut(s)	410, 2875
XceI	RCATGY	7 cut(s)	303, 1106, 1621, 1875, 1935, 3629, 3934
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1073
XhoI	CTCGAG	1 cut(s)	3207
XmiI	GTMKAC	1 cut(s)	2719
XmnI	GAANNNNTTC	3 cut(s)	1736, 2431, 3482
XspI	CTAG	6 cut(s)	387, 411, 720, 1688, 2858, 2876
Zsp2I	ATGCAT	3 cut(s)	2278, 2952, 3631

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.