Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7A

Orthogroup ID

OG0002992

Physical Location & Seq

Genomic Coordinates Forward (+)

15881310 .. 15893981

Gene Structure 12672 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7AG179300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4419 bp

ATGTATGCTATACTGTTTAACGCTCATGGCTATCTATGTAGGTTGCTTGATTTGTTTCTGCAAAATATAGAAGTAATAGAGCCCATGGAAGATGAATTGTATAATGAAGAACTAGTGCCATGTGCTTGCATAACTGGGGTACATCTAAAGGTTTCTACTCGGGAAGATACGGAGAAAATATCAACATTATTAATCGAGGCAGCTGCCCAGGTTAATGATCCAAAGTTGGGACTTCCAAATCCAACGAATCAGTGTTCTACTTGTGGTACCCAAGATATAAAATCCTGTGATGGTCATTTTGGGTCCATCAAGTTTCCATGCACGATACTCCATCCATACTATCTACCGGAAGTTGCTCTCATTTTAAACAAGATTTGCCCGGCATGTAAAACCATCAGGCAAGGTGTAAGGGTCAAGGGATCTGAACGGAGGGGCTGCAGATATTGCTTTGGAGGTTCATCGGATTACCCTAGAATGAAGTTCAAAGTTTCTTCTAGAGAGCTTTTCAGAAGAAGTACAATAATAGTAGAAGCAGTTGGAGAATTGCCTCCTGATTATTGGGACTTCATTCCCAAAGATCCCCAACATGATGAAACACACACAAAACCAAATAGAAGAGTTCTATCTCATGCCCAGGTTCATTTTTTGTTGAATGATGTTGATCCAGATTTCATCACAAAGTTTGTTCCAACAACCAGCTCACTTTCTTTGGACTGTTTCCTTGTCACGCCAAATTGTCATCGTGTGACAGAAGTTATGTATTCATTTAATAATGGGCAGTCATTGATGTTTGATGATCGGACTAGAGCTTACAGGAAACTAGTGGATTTCAGAGGCACTGCGAACGAGCTGGGTTCTCGTGTTTTGGACTGCCTGAAAATTTCCAAGATAAACAAAGACAAAACAGGCAGAGATTCAATTTCAGTTGAGCAACAAAAGATTAAGGATTCTCCGTCCAGAACATCCGGCCTGAGATGGATAAAAGATGTTGTTCTTGGAAAGAGAAGTGATCACTGTTTTCGAACTGTTGTCGTTGGTGATCCAAACATCAAGCTGAGTGAGATTGGCATACCCCGCCATATTGCAGAGAAAATGCAGATCTCTGAGAATCTGAACCAATATAACCTTGAGAAGTTGTATGCGTTTTGCCAGCTACGCCTTGGCCATCAGGGGAGAGGTGACATACATGTTCGTAGAAATGATAGTCTGGTTAGGATAAGTAATTTAGAAGAACTGGAGATGGGAGATATTATATACAGGGCGTTGAGTGATGGAGATGTTGTGTTGATAAATAGGCCTCCATCAATACATCAACACTCCCTAATAGCTTTAACCGCAAAGGTCCTTCCAGTAAACTCTGTAGTGTCGATAAATCCACTGTGTTGCTCTCCTTTTCGTGGTGATTTTGATGGTGATTGCCTGCATGGTTATGTTCCTCAATCAGTGGATACCAGAGTCGAGCTTACAGAGCTTGTTGCTTTAGATAAGCAATTAGTTAATGGGCAGAGTGGTTCCAACCTTCTTTCTCTCAGTCAAGATAGTTTAACTGCTTGCTATCTCATCATGGAAGATGGCACCCTGATGAATAAGTTTCAAATGCAACAACTGAAAATGCTTTGTCCCCCTGAACTGCCATCACCAGCGATAATTAAAGATCCTTCTGGTAACTCCGTTTCTTGGACGGCAAAGCAGATAATCAGCATGTTTCTACCTGCAAATTTTAATTATGCTTTTCCTTCAAATGGTGTGCATATTAGCAATGGAGAGCTTCTAGCTTCTTCTGAAGGTTCTTCTTGGCTGCGAGACACCAGTGGGAATCTTTTCCAAAGTCTCATCGAACATTGCCCCGGTCAGATACTGGACATCTTCTTTACTGCTCAGGGAGTTCTATGTGAGTGGTTGTCAATGAGGGGCTTGAGTGTTTCTCTCTCAGACTTGTATATCGCGTCGGATACATGTTCAAGGAAAAACTTGGTGGAGGAAATCTTCTATGGGTTACAAGAAGCAGAGGAGGCATGTACCATCAGACAGTTGATGCTTGATTCTTGTCAGGAATTTCTTATGGGCTATGCTGAAGAGACAAAAAGTCCTTTGACTTTTGGTTCAGAACATTTTTGTTATCAGAAGCAGAAATCTGCTGCACTTAGTCAGATCACGGTAGATGCTTTCAAAGAAGGTTTCCGGGATGTCCAAAATTTAGCATACAAATATGCCCGTGAGGACAACTCGTTGCTGGCTATGTTTAAGGCTGGAAGCAAGGGTAATATGCTGAAATTAGTTCAGCATGGTATGTGCCTTGGTTTGCAACATTCACTGGTTCCCTTATCATTCAAGTTTCCGAACCAGCTCTCATGTGATGCATGGAATGATCAAAAAGCACGTGGTATCGTTCAGGAGGTTGGAAGAGCTTTTGAATCCATCGAGTCATATATCCCATCCGCTGTCGTTAAAAGTTCGTTCTTGACTGGGCTGAATCCGGTCGAATGCTTTGTTCATTCAGTCACGAGTCGAGATGGTTCTTTTAGTAATAATGCGGAACTTCCTGGGACTCTGAATCGAAAGCTCATGTATTTCATGCGTGATTTGCATACTGCTTATGACGGAACAGTTAGAAACGCATATGGGAACCAGCTGGTTCAGTTCTCCTATGATAATGACGAGGGCATATCTACTGCAGATAGCACCACAAACAGCTCATATGCAAAGAGTGTTATTGATTGTGGTGGCGGCCAACCCGTTGGTGCATTGTCTGCTTGTGCAATATCTGAAGCTGCATACAGTGCTTTAGATCAGCCAGTTAGTCTACTTGAAACTTCTCCTCTGCTAAATCTGAAGAACATTTTGGAGTGTGGTTCAAAGAAAAGGAGTGCTAAGCAAACTATGTCATTATTTTTATCTGAGAAGCTTGGAAGACATAGGCATGGTTTCGAGTATGGAGCGCTAGAAGTTAAAAATCATCTAGAGGGACTCACATTTTCAGACATCGTTTCTACTGTCATGATAATGTTCTCCTCACAAACTGGCAGCAAGATGCATTTTAGTCCTTGGGTTTCTCACTTTCATATATCTAAGGAAATTGTACGAAGAAGAAGGCTGAATATGCGATCTATTATAGAGGCACTTTATGCAAGATGCAACTCCGCTGGAGCGGAGTTGAAAATTATCCTTTGGAATTTGAAGATCACAAGAAATAAGGAATGTGATTGCTGCAGCACACCTAATGATACATTTTGCATCAGTGTCACACTGACTGAAAAGCACGACAATTCTTCTGAAGAACTGGATAAAACTCGAGTCTTGGTGATACCATTCTTGCTTAAAACAGTAGTTAAAGGTTTTCTGGAGATAAAAAAGGTGGATATTATATGGAATGATCGACCTGGTTCACCTGGTGAACTCTACTTGAGAGTTTCTATGTCAGGAAAGACCAGAAGGGGGGCTACCTTTTGGAATATGCTTATGGACGATTGTCTTCCGGTTATGGATATGATTGATTGTTCACGAAGTCATCCAGATAACGTCGATGACTTCTGCACTGCCTATGGAATAGATGTTGGGTGGAAACATTTCCTTAATAAGTTGAAGTCTGCAACAGTAGATATTGGAAAGACAATTCTTCCTCAGCATTTGCTTCTTGCAGCAGATTGTCTGTCAGCCACCGGCGAGTTTGTCAGCTTAAACGCAAAAGGCATTGCTCAACAATTGGAACATGCATCTGTCACAGCCCCTTTTATGCAAGCTTGCTTTTCTACTCCTGGTCCTTGCTTGGTTAAAGCTGCTAAGGCTGGAGTGGTGGATAATCTCCAGGGGAGTTTGGACGCATTGACATGGGGAAATACTCCGTCCTTGGGAAGTGGTGGACAGTTTGATATTATATTTGCTGGGGAGGGATCTGTATCTTCTAAGCCATTTGATGTTCATGATATGCTAAGCAGACAAATCAGTTCCAACCCCCAGAATGTATTTGAAATGTCTGATGCTCCAATTCATCTATCTGACAAATATGTTGCTCCACATGTGTACAAGTATGGTCTTTTGTGCAAGTTGAAGAGTATTACCAAATCAGTCTTAAGAGAAACTTTTACGATCCAGGATATTGATAGATTGTCTCAAACCTTGAAGAATATATTGCACAAGTATGACATCAATGAAAGCATAAATGAGAAGGAGAAGAAAGATTTGATGACGGCTTTAGAGTTTCACCCTCATAGGGATCGTAAGATCGGATTAGGAATCAAAGATATAAAGGTTGGCCAGCACCCTAACCATGAGAATGCGCGATGCTTCATGCTAGTACATACGGACGGGACGATTGAAGATGTTTCGTACCACAAGTGCATCATTGGTGCTTTTGATATCATAGCTCCTCATAGGGCAAAGGGCTATCGCTTGAGACATTTGAGCCGTGGTCCTCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000418 GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010495 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901698 GO:1901699 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1472

Amino Acids

164.31

Weight (kDa)

6.72

Isoelectric Point (pI)

43.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	70 - 132	4.3e-07	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	333 - 492	3.5e-35	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	497 - 646	1.1e-19	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	713 - 770	2.9e-08	RNA polymerase Rpb1, domain 4
DUF3223	PF11523	1374 - 1447	2.3e-25	Protein of unknown function (DUF3223)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002992)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63020 AT1G63020 AT1G63020 AT1G63020 AT1G63020
fragaria_vesca	FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400
malus_domestica	MD04G1068800.v1.1 MD06G1069300.v1.1 MD06G1069400.v1.1 MD06G1069500.v1.1 MD06G1069800.v1.1
prunus_persica	Prupe.5G089300_v2.0.a1
pyrus_communis	pycom04g06250
rosa_chinensis	RchiOBHm_Chr7g0199481
rosa_laevigata	RLG00000003790
rosa_multiflora	Rmu_co8411067.1_g000001 Rmu_sc0003890.1_g000001
rosa_roxburghii	Rroxscaffold_3G00256700
rosa_rugosa	Rorug07G0054300 Rorug07G0054400 Rorug07G0054500 Rorug07G0054500
rosa_samantha	Rh7AG179300 Rh7BG182800 Rh7CG188100 Rh7DG182200
rosa_wichuraiana	Rw7G015760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1720
Acc65I	GGTACC	1 cut(s)	266
AccB1I	GGYRCC	2 cut(s)	266, 1574
AccBSI	CCGCTC	1 cut(s)	3149
AccI	GTMKAC	1 cut(s)	2803
AccII	CGCG	2 cut(s)	1946, 4279
AciI	CCGC	7 cut(s)	1075, 1335, 2439, 2534, 2727, 3141, 3149
AclWI	GGATC	9 cut(s)	212, 427, 572, 656, 1034, 1649, 3898, 4081, 4221
AcoI	YGGCCR	3 cut(s)	1162, 2728, 4252
AcsI	RAATTY	5 cut(s)	879, 1717, 2054, 2194, 3172
AcuI	CTGAAG	5 cut(s)	1803, 2094, 2787, 2852, 3294
AcvI	CACGTG	1 cut(s)	2381
AdeI	CACNNNGTG	1 cut(s)	3392
AfaI	GTAC	8 cut(s)	141, 268, 517, 2020, 3081, 4022, 4296, 4328
AfeI	AGCGCT	1 cut(s)	2940
AfiI	CCNNNNNNNGG	7 cut(s)	227, 1397, 1677, 1742, 3435, 3848, 4210
AflII	CTTAAG	1 cut(s)	4069
AflIII	ACRYGT	3 cut(s)	1186, 1955, 4015
AhdI	GACNNNNNGTC	1 cut(s)	2085
AhlI	ACTAGT	2 cut(s)	112, 820
AjnI	CCWGG	8 cut(s)	207, 633, 2542, 3379, 3388, 3754, 3804, 4089
AjuI	GAANNNNNNNTTGG	2 cut(s)	2825, 2857
Alw26I	GTCTC	5 cut(s)	1797, 1835, 2072, 4113, 4387
AlwI	GGATC	9 cut(s)	212, 427, 572, 656, 1034, 1649, 3898, 4081, 4221
AlwNI	CAGNNNCTG	1 cut(s)	1858
Ama87I	CYCGRG	2 cut(s)	159, 3291
Aor51HI	AGCGCT	1 cut(s)	2940
AoxI	GGCC	5 cut(s)	967, 1162, 1295, 2728, 4252
ApoI	RAATTY	5 cut(s)	879, 1717, 2054, 2194, 3172
AseI	ATTAAT	1 cut(s)	191
Asp700I	GAANNNNTTC	2 cut(s)	1820, 3566
Asp718I	GGTACC	1 cut(s)	266
AspLEI	GCGC	2 cut(s)	2941, 4279
AspS9I	GGNCC	4 cut(s)	303, 1342, 3758, 4409
AsuC2I	CCSGG	3 cut(s)	380, 1848, 2183
AsuHPI	GGTGA	9 cut(s)	1049, 1190, 1412, 1424, 1629, 3313, 3378, 3404, 4193
AsuII	TTCGAA	1 cut(s)	1021
AvaI	CYCGRG	2 cut(s)	159, 3291
AvaII	GGWCC	4 cut(s)	303, 1342, 3758, 4409
BaeI	ACNNNNGTAYC	2 cut(s)	1440, 1473
BalI	TGGCCA	2 cut(s)	1164, 4254
BanI	GGYRCC	2 cut(s)	266, 1574
BanII	GRGCYC	1 cut(s)	84
BarI	GAAGNNNNNNTAC	2 cut(s)	3424, 3456
BauI	CACGAG	2 cut(s)	858, 2503
BbrPI	CACGTG	1 cut(s)	2381
BbsI	GAAGAC	2 cut(s)	2917, 3464
BbvCI	CCTCAGC	1 cut(s)	3621
BceAI	ACGGC	3 cut(s)	1698, 4203, 4389
BcgI	CGANNNNNNTGC	2 cut(s)	185, 219
BciT130I	CCWGG	8 cut(s)	209, 635, 2544, 3381, 3390, 3756, 3806, 4091
BciVI	GTATCC	2 cut(s)	1441, 1945
BclI	TGATCA	2 cut(s)	1009, 2368
BcnI	CCSGG	3 cut(s)	380, 1848, 2183
BcoDI	GTCTC	5 cut(s)	1797, 1835, 2072, 4113, 4387
BcuI	ACTAGT	2 cut(s)	112, 820
BfaI	CTAG	9 cut(s)	113, 471, 495, 804, 821, 1772, 2942, 2960, 4292
BfmI	CTRYAG	4 cut(s)	436, 1359, 2673, 3208
BfoI	RGCGCY	1 cut(s)	2942
BfrI	CTTAAG	1 cut(s)	4069
BfuAI	ACCTGC	1 cut(s)	1720
BfuI	GTATCC	2 cut(s)	1441, 1945
BglII	AGATCT	1 cut(s)	1098
BlpI	GCTNAGC	2 cut(s)	2871, 3929
Bme18I	GGWCC	4 cut(s)	303, 1342, 3758, 4409
BmeRI	GACNNNNNGTC	1 cut(s)	2085
BmeT110I	CYCGRG	2 cut(s)	159, 3291
BmgT120I	GGNCC	4 cut(s)	303, 1342, 3758, 4409
BmiI	GGNNCC	6 cut(s)	268, 304, 1513, 1576, 2319, 2627
BmrI	ACTGGG	2 cut(s)	144, 2475
BmuI	ACTGGG	2 cut(s)	144, 2475
BoxI	GACNNNNGTC	1 cut(s)	3468
BpiI	GAAGAC	2 cut(s)	2917, 3464
BplI	GAGNNNNNCTC	4 cut(s)	1909, 1941, 2210, 2242
BpmI	CTGGAG	5 cut(s)	1256, 3165, 3362, 3788, 3807
Bpu10I	CCTNAGC	3 cut(s)	1878, 3621, 3780
Bpu1102I	GCTNAGC	2 cut(s)	2871, 3929
Bpu14I	TTCGAA	1 cut(s)	1021
BpuEI	CTTGAG	4 cut(s)	1148, 1936, 3424, 4411
BpuMI	CCSGG	3 cut(s)	380, 1848, 2183
BsaAI	YACGTR	1 cut(s)	2381
BsaBI	GATNNNNATC	2 cut(s)	660, 3895
BsaWI	WCCGGW	3 cut(s)	346, 2476, 3475
BsaXI	ACNNNNNCTCC	2 cut(s)	1266, 1296
Bsc4I	CCNNNNNNNGG	7 cut(s)	227, 1397, 1677, 1742, 3435, 3848, 4210
Bse118I	RCCGGY	1 cut(s)	3659
Bse3DI	GCAATG	3 cut(s)	1765, 1840, 3690
Bse8I	GATNNNNATC	2 cut(s)	660, 3895
BseBI	CCWGG	8 cut(s)	209, 635, 2544, 3381, 3390, 3756, 3806, 4091
BseGI	GGATG	5 cut(s)	331, 962, 2191, 2435, 3508
BseJI	GATNNNNATC	2 cut(s)	660, 3895
BseLI	CCNNNNNNNGG	7 cut(s)	227, 1397, 1677, 1742, 3435, 3848, 4210
BseMI	GCAATG	3 cut(s)	1765, 1840, 3690
BseMII	CTCAG	8 cut(s)	962, 1046, 1095, 1543, 1892, 1944, 2889, 3635
BseRI	GAGGAG	4 cut(s)	2024, 2808, 3001, 4356
BseYI	CCCAGC	2 cut(s)	850, 3881
BsgI	GTGCAG	2 cut(s)	2124, 3517
Bsh1236I	CGCG	2 cut(s)	1946, 4279
Bsh1285I	CGRYCG	1 cut(s)	2481
BshFI	GGCC	5 cut(s)	969, 1164, 1297, 2730, 4254
BshNI	GGYRCC	2 cut(s)	266, 1574
BsiEI	CGRYCG	1 cut(s)	2481
BsiHKCI	CYCGRG	2 cut(s)	159, 3291
BsiSI	CCGG	8 cut(s)	347, 380, 966, 1848, 2182, 2477, 3476, 3660
BslFI	GGGAC	6 cut(s)	243, 575, 1605, 2560, 2979, 4321
BslI	CCNNNNNNNGG	7 cut(s)	227, 1397, 1677, 1742, 3435, 3848, 4210
BsmAI	GTCTC	5 cut(s)	1797, 1835, 2072, 4113, 4387
BsmFI	GGGAC	6 cut(s)	243, 575, 1605, 2560, 2979, 4321
BsmI	GAATGC	2 cut(s)	2489, 4279
BsnI	GGCC	5 cut(s)	969, 1164, 1297, 2730, 4254
BsoBI	CYCGRG	2 cut(s)	159, 3291
Bsp119I	TTCGAA	1 cut(s)	1021
Bsp1286I	GDGCHC	1 cut(s)	84
Bsp1407I	TGTACA	1 cut(s)	4020
Bsp1720I	GCTNAGC	2 cut(s)	2871, 3929
Bsp19I	CCATGG	1 cut(s)	84
BspACI	CCGC	7 cut(s)	1075, 1335, 2439, 2534, 2727, 3141, 3149
BspANI	GGCC	5 cut(s)	969, 1164, 1297, 2730, 4254
BspCNI	CTCAG	8 cut(s)	963, 1047, 1096, 1542, 1891, 1943, 2890, 3634
BspFNI	CGCG	2 cut(s)	1946, 4279
BspHI	TCATGA	2 cut(s)	2997, 3919
BspLI	GGNNCC	6 cut(s)	268, 304, 1513, 1576, 2319, 2627
BspMAI	CTGCAG	3 cut(s)	440, 2677, 3212
BspMI	ACCTGC	1 cut(s)	1720
BspPI	GGATC	9 cut(s)	212, 427, 572, 656, 1034, 1649, 3898, 4081, 4221
BspQI	GCTCTTC	1 cut(s)	2398
BspT104I	TTCGAA	1 cut(s)	1021
BspT107I	GGYRCC	2 cut(s)	266, 1574
BspTI	CTTAAG	1 cut(s)	4069
BsrBI	CCGCTC	1 cut(s)	3149
BsrDI	GCAATG	3 cut(s)	1765, 1840, 3690
BsrFI	RCCGGY	1 cut(s)	3659
BsrGI	TGTACA	1 cut(s)	4020
BssAI	RCCGGY	1 cut(s)	3659
BssSI	CACGAG	2 cut(s)	858, 2503
BssT1I	CCWWGG	5 cut(s)	84, 1159, 2296, 3044, 3846
Bst2BI	CACGAG	2 cut(s)	858, 2503
Bst2UI	CCWGG	8 cut(s)	209, 635, 2544, 3381, 3390, 3756, 3806, 4091
Bst6I	CTCTTC	4 cut(s)	610, 2070, 2398, 4043
BstAFI	CTTAAG	1 cut(s)	4069
BstAPI	GCANNNNNTGC	4 cut(s)	444, 2750, 2780, 3125
BstAUI	TGTACA	1 cut(s)	4020
BstBAI	YACGTR	1 cut(s)	2381
BstBI	TTCGAA	1 cut(s)	1021
BstC8I	GCNNGC	8 cut(s)	127, 1151, 1421, 1552, 2235, 3738, 3742, 4256
BstDSI	CCRYGG	2 cut(s)	84, 4405
BstF5I	GGATG	5 cut(s)	331, 962, 2191, 2435, 3508
BstFNI	CGCG	2 cut(s)	1946, 4279
BstH2I	RGCGCY	1 cut(s)	2942
BstHHI	GCGC	2 cut(s)	2941, 4279
BstMAI	GTCTC	5 cut(s)	1797, 1835, 2072, 4113, 4387
BstMCI	CGRYCG	1 cut(s)	2481
BstNI	CCWGG	8 cut(s)	209, 635, 2544, 3381, 3390, 3756, 3806, 4091
BstNSI	RCATGY	7 cut(s)	387, 1190, 1705, 1959, 2019, 3713, 4019
BstPAI	GACNNNNGTC	1 cut(s)	3468
BstSFI	CTRYAG	4 cut(s)	436, 1359, 2673, 3208
BstUI	CGCG	2 cut(s)	1946, 4279
BstV2I	GAAGAC	2 cut(s)	2917, 3464
BstX2I	RGATCY	5 cut(s)	419, 577, 1098, 1654, 3890
BstXI	CCANNNNNNTGG	1 cut(s)	2738
BstYI	RGATCY	5 cut(s)	419, 577, 1098, 1654, 3890
BsuI	GTATCC	2 cut(s)	1441, 1945
BsuRI	GGCC	5 cut(s)	969, 1164, 1297, 2730, 4254
BtgI	CCRYGG	2 cut(s)	84, 4405
BtgZI	GCGATG	1 cut(s)	4294
BtsCI	GGATG	5 cut(s)	331, 962, 2191, 2435, 3508
BtsI	GCAGTG	2 cut(s)	837, 3534
BveI	ACCTGC	1 cut(s)	1720
Cac8I	GCNNGC	8 cut(s)	127, 1151, 1421, 1552, 2235, 3738, 3742, 4256
CaiI	CAGNNNCTG	1 cut(s)	1858
CciI	TCATGA	2 cut(s)	2997, 3919
CfoI	GCGC	2 cut(s)	2941, 4279
Cfr10I	RCCGGY	1 cut(s)	3659
Cfr13I	GGNCC	4 cut(s)	303, 1342, 3758, 4409
CseI	GACGC	2 cut(s)	1935, 3827
CsiI	ACCWGGT	2 cut(s)	3379, 3388
Csp6I	GTAC	8 cut(s)	140, 267, 516, 2019, 3080, 4021, 4295, 4327
CviQI	GTAC	8 cut(s)	140, 267, 516, 2019, 3080, 4021, 4295, 4327
DraI	TTTAAA	1 cut(s)	366
DraIII	CACNNNGTG	1 cut(s)	3392
DriI	GACNNNNNGTC	1 cut(s)	2085
EaeI	YGGCCR	3 cut(s)	1162, 2728, 4252
Eam1104I	CTCTTC	4 cut(s)	610, 2070, 2398, 4043
Eam1105I	GACNNNNNGTC	1 cut(s)	2085
EarI	CTCTTC	4 cut(s)	610, 2070, 2398, 4043
Eco130I	CCWWGG	5 cut(s)	84, 1159, 2296, 3044, 3846
Eco147I	AGGCCT	1 cut(s)	1297
Eco24I	GRGCYC	1 cut(s)	84
Eco32I	GATATC	1 cut(s)	4357
Eco47I	GGWCC	4 cut(s)	303, 1342, 3758, 4409
Eco47III	AGCGCT	1 cut(s)	2940
Eco57I	CTGAAG	5 cut(s)	1803, 2094, 2787, 2852, 3294
Eco72I	CACGTG	1 cut(s)	2381
Eco88I	CYCGRG	2 cut(s)	159, 3291
EcoO109I	RGGNCCY	1 cut(s)	1342
EcoRII	CCWGG	8 cut(s)	207, 633, 2542, 3379, 3388, 3754, 3804, 4089
EcoRV	GATATC	1 cut(s)	4357
EcoT14I	CCWWGG	5 cut(s)	84, 1159, 2296, 3044, 3846
EcoT22I	ATGCAT	3 cut(s)	2362, 3036, 3715
EcoT38I	GRGCYC	1 cut(s)	84
ErhI	CCWWGG	5 cut(s)	84, 1159, 2296, 3044, 3846
FalI	AAGNNNNNCTT	2 cut(s)	4063, 4095
FaqI	GGGAC	6 cut(s)	243, 575, 1605, 2560, 2979, 4321
FauI	CCCGC	1 cut(s)	1082
FauNDI	CATATG	2 cut(s)	2620, 2698
FbaI	TGATCA	2 cut(s)	1009, 2368
FblI	GTMKAC	1 cut(s)	2803
FokI	GGATG	5 cut(s)	318, 949, 2198, 2422, 3495
FriOI	GRGCYC	1 cut(s)	84
FspBI	CTAG	9 cut(s)	113, 471, 495, 804, 821, 1772, 2942, 2960, 4292
GlaI	GCGC	2 cut(s)	2940, 4278
GsaI	CCCAGC	2 cut(s)	854, 3885
GsuI	CTGGAG	5 cut(s)	1256, 3165, 3362, 3788, 3807
HaeII	RGCGCY	1 cut(s)	2942
HaeIII	GGCC	5 cut(s)	969, 1164, 1297, 2730, 4254
HapII	CCGG	8 cut(s)	347, 380, 966, 1848, 2182, 2477, 3476, 3660
HgaI	GACGC	2 cut(s)	1935, 3827
HhaI	GCGC	2 cut(s)	2941, 4279
Hin6I	GCGC	2 cut(s)	2939, 4277
HinP1I	GCGC	2 cut(s)	2939, 4277
HindIII	AAGCTT	2 cut(s)	2903, 3738
HpaII	CCGG	8 cut(s)	347, 380, 966, 1848, 2182, 2477, 3476, 3660
HphI	GGTGA	9 cut(s)	1049, 1190, 1412, 1424, 1629, 3313, 3378, 3404, 4193
Hpy166II	GTNNAC	7 cut(s)	1354, 2804, 3386, 3395, 3500, 3860, 4021
Hpy8I	GTNNAC	7 cut(s)	1354, 2804, 3386, 3395, 3500, 3860, 4021
Hpy99I	CGWCG	2 cut(s)	1951, 3524
HpyAV	CCTTC	9 cut(s)	1355, 1529, 1668, 1746, 1778, 2168, 3084, 3426, 4159
HpyCH4IV	ACGT	2 cut(s)	2380, 3519
HpySE526I	ACGT	2 cut(s)	2380, 3519
HspAI	GCGC	2 cut(s)	2939, 4277
KpnI	GGTACC	1 cut(s)	270
Ksp22I	TGATCA	2 cut(s)	1009, 2368
LguI	GCTCTTC	1 cut(s)	2398
LmnI	GCTCC	5 cut(s)	2936, 3146, 3985, 4015, 4369
MabI	ACCWGGT	2 cut(s)	3379, 3388
MaeI	CTAG	9 cut(s)	113, 471, 495, 804, 821, 1772, 2942, 2960, 4292
MaeII	ACGT	2 cut(s)	2380, 3519
MaeIII	GTNAC	8 cut(s)	724, 745, 1178, 1664, 1995, 2500, 3241, 3718
MbiI	CCGCTC	1 cut(s)	3149
MfeI	CAATTG	1 cut(s)	3701
MflI	RGATCY	5 cut(s)	419, 577, 1098, 1654, 3890
MhlI	GDGCHC	1 cut(s)	84
MlsI	TGGCCA	2 cut(s)	1164, 4254
MluNI	TGGCCA	2 cut(s)	1164, 4254
MlyI	GAGTC	6 cut(s)	1464, 2432, 2515, 2542, 2961, 3303
MmeI	TCCRAC	7 cut(s)	266, 517, 713, 1539, 1929, 2380, 3972
Mox20I	TGGCCA	2 cut(s)	1164, 4254
Mph1103I	ATGCAT	3 cut(s)	2362, 3036, 3715
MroXI	GAANNNNTTC	2 cut(s)	1820, 3566
MscI	TGGCCA	2 cut(s)	1164, 4254
MslI	CAYNNNNRTG	7 cut(s)	1428, 1580, 2919, 3002, 3826, 4137, 4272
Msp20I	TGGCCA	2 cut(s)	1164, 4254
MspA1I	CMGCKG	4 cut(s)	203, 2441, 2632, 3143
MspCI	CTTAAG	1 cut(s)	4069
MspI	CCGG	8 cut(s)	347, 380, 966, 1848, 2182, 2477, 3476, 3660
MunI	CAATTG	1 cut(s)	3701
Mva1269I	GAATGC	2 cut(s)	2489, 4279
MvaI	CCWGG	8 cut(s)	209, 635, 2544, 3381, 3390, 3756, 3806, 4091
MvnI	CGCG	2 cut(s)	1946, 4279
NciI	CCSGG	3 cut(s)	380, 1848, 2183
NcoI	CCATGG	1 cut(s)	84
NdeI	CATATG	2 cut(s)	2620, 2698
NlaIV	GGNNCC	6 cut(s)	268, 304, 1513, 1576, 2319, 2627
NmuCI	GTSAC	6 cut(s)	724, 745, 1178, 2500, 3241, 3718
NsiI	ATGCAT	3 cut(s)	2362, 3036, 3715
NspI	RCATGY	7 cut(s)	387, 1190, 1705, 1959, 2019, 3713, 4019
NspV	TTCGAA	1 cut(s)	1021
PaeR7I	CTCGAG	1 cut(s)	3291
PagI	TCATGA	2 cut(s)	2997, 3919
PceI	AGGCCT	1 cut(s)	1297
PciI	ACATGT	3 cut(s)	1186, 1955, 4015
PciSI	GCTCTTC	1 cut(s)	2398
PcsI	WCGNNNNNNNCGW	1 cut(s)	4307
PctI	GAATGC	2 cut(s)	2489, 4279
PdmI	GAANNNNTTC	2 cut(s)	1820, 3566
PfoI	TCCNGGA	2 cut(s)	2181, 4089
PleI	GAGTC	6 cut(s)	1463, 2431, 2514, 2542, 2961, 3302
PmaCI	CACGTG	1 cut(s)	2381
PmlI	CACGTG	1 cut(s)	2381
PpsI	GAGTC	6 cut(s)	1463, 2431, 2514, 2542, 2961, 3302
Ppu21I	YACGTR	1 cut(s)	2381
PpuMI	RGGWCCY	1 cut(s)	1342
PscI	ACATGT	3 cut(s)	1186, 1955, 4015
PshAI	GACNNNNGTC	1 cut(s)	3468
PshBI	ATTAAT	1 cut(s)	191
Psp5II	RGGWCCY	1 cut(s)	1342
Psp6I	CCWGG	8 cut(s)	207, 633, 2542, 3379, 3388, 3754, 3804, 4089
PspCI	CACGTG	1 cut(s)	2381
PspFI	CCCAGC	2 cut(s)	850, 3881
PspGI	CCWGG	8 cut(s)	207, 633, 2542, 3379, 3388, 3754, 3804, 4089
PspN4I	GGNNCC	6 cut(s)	268, 304, 1513, 1576, 2319, 2627
PspPI	GGNCC	4 cut(s)	303, 1342, 3758, 4409
PspPPI	RGGWCCY	1 cut(s)	1342
PspXI	VCTCGAGB	1 cut(s)	3291
PstI	CTGCAG	3 cut(s)	440, 2677, 3212
PstNI	CAGNNNCTG	1 cut(s)	1858
PsuI	RGATCY	5 cut(s)	419, 577, 1098, 1654, 3890
PvuII	CAGCTG	2 cut(s)	203, 2632
RsaI	GTAC	8 cut(s)	141, 268, 517, 2020, 3081, 4022, 4296, 4328
RsaNI	GTAC	8 cut(s)	140, 267, 516, 2019, 3080, 4021, 4295, 4327
RseI	CAYNNNNRTG	7 cut(s)	1428, 1580, 2919, 3002, 3826, 4137, 4272
SapI	GCTCTTC	1 cut(s)	2398
Sau96I	GGNCC	4 cut(s)	303, 1342, 3758, 4409
SchI	GAGTC	6 cut(s)	1464, 2432, 2515, 2542, 2961, 3303
SduI	GDGCHC	1 cut(s)	84
SexAI	ACCWGGT	2 cut(s)	3379, 3388
SfcI	CTRYAG	4 cut(s)	436, 1359, 2673, 3208
Sfr274I	CTCGAG	1 cut(s)	3291
SfuI	TTCGAA	1 cut(s)	1021
SgrAI	CRCCGGYG	1 cut(s)	3659
SinI	GGWCC	4 cut(s)	303, 1342, 3758, 4409
SlaI	CTCGAG	1 cut(s)	3291
SmiMI	CAYNNNNRTG	7 cut(s)	1428, 1580, 2919, 3002, 3826, 4137, 4272
SmlI	CTYRAG	6 cut(s)	1127, 1915, 3291, 3403, 4069, 4390
SmoI	CTYRAG	6 cut(s)	1127, 1915, 3291, 3403, 4069, 4390
SpeI	ACTAGT	2 cut(s)	112, 820
SseBI	AGGCCT	1 cut(s)	1297
SsiI	CCGC	7 cut(s)	1075, 1335, 2439, 2534, 2727, 3141, 3149
SspMI	CTAG	9 cut(s)	113, 471, 495, 804, 821, 1772, 2942, 2960, 4292
StuI	AGGCCT	1 cut(s)	1297
StyI	CCWWGG	5 cut(s)	84, 1159, 2296, 3044, 3846
TaiI	ACGT	2 cut(s)	2383, 3522
TatI	WGTACW	3 cut(s)	515, 4020, 4294
TauI	GCSGC	1 cut(s)	2730
TseFI	GTSAC	6 cut(s)	724, 745, 1178, 2500, 3241, 3718
Tsp45I	GTSAC	6 cut(s)	724, 745, 1178, 2500, 3241, 3718
TspGWI	ACGGA	7 cut(s)	185, 442, 942, 1660, 2616, 3831, 4316
Vha464I	CTTAAG	1 cut(s)	4069
VpaK11BI	GGWCC	4 cut(s)	303, 1342, 3758, 4409
VspI	ATTAAT	1 cut(s)	191
XapI	RAATTY	5 cut(s)	879, 1717, 2054, 2194, 3172
XbaI	TCTAGA	2 cut(s)	494, 2959
XceI	RCATGY	7 cut(s)	387, 1190, 1705, 1959, 2019, 3713, 4019
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1157
XhoI	CTCGAG	1 cut(s)	3291
XmiI	GTMKAC	1 cut(s)	2803
XmnI	GAANNNNTTC	2 cut(s)	1820, 3566
XspI	CTAG	9 cut(s)	113, 471, 495, 804, 821, 1772, 2942, 2960, 4292
Zsp2I	ATGCAT	3 cut(s)	2362, 3036, 3715

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.