Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0002992

Physical Location & Seq

Genomic Coordinates Reverse (-)

52757007 .. 52764274

Gene Structure 7268 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000003790

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3693 bp

ATGGAAGATGAATTGTATAATGAAGAACTAGTGCCATGTGCTTGCATAACTGGGGTACATCTAAATGTTTCTACTCGGGAAGATACGGAGAAAATATCAACATTATTAATCGAGGCAGCTGCCCAGGTTAATGATCCAAAGTTGGGACTTCCAAATCCAACGAATCAGTGTTCTACTTGTGGTACCCAAGATATAAAATCCTGTGATGGTCATTTTGGGTCCATCAAGTTTCCATGCACGATACTCCATCCATACTATCTACCGGAAGTTGCTCTTATTTTAAACAAGATTTGCCCGGCATGTAAAACCATCAGGCAAGGTGTAAGGGTCAAGGGCTCTGAACGGAGGGGCTGCAGATATTGCTTTGGAGGTTCATCGGATTACCCTAGAATGAAGTTCAAAGTTTCTTCTAGAGAGCTTTTCAGAAGAAGTACAATAATAGTAGAAGCAGTTGGAGAATTGCCTCCTGATTATTGGGACTTCATTCCCAAAGATCCCCAACATGATGAAACACACACAAAACCAAATAGAAGAGTTCTATCTCATGCCCAGGTTCATTTTTTGTTGAATGATGTTGATCCAGATTTCATCACAAAGTTTGTTCCAACAACCAGCTCACTTTCTTTGGACTGTTTCCTTGTCACTCCAAATTGTCATCGTGTGACAGAAGTTATGTATTCATTTAATAATGGGCAGTCATTGATGTTCGATGATCGGACTAGAGCTTACAGGAAACTAGTGGATTTCAGAGGCACTGCGAACGAGCTGGGTTCTCGTGTTTTGGACTGCCTGAAAATTTCCAAGATAAACAAAGACAAAACAGGCAGAGATTCAATTTCAGTTGAGCAACAAAAGATTAAGGATTCTCCGTCCAGAACATCTGGCTTGAGATGGATAAAAGATGTTGTTCTTGGAAAGAGAAGTGATCACTGTTTTCGAACTGTTGTTGTTGGTGATCCAAACATCAAGCTGAGTGAGATTGGCATACCCCGCCATATTGCAGAGAAAATGCAGATCTCTGAGAATCTGAACCAATATAACCTTCAGAAGTTGTATGCGTTTTGCCAGCTACGCCTTGGCCATCAGGGGAGAGGTGACATACATGTTCGTAGAAATGATAGTCTGGTTAGGATAAGTAATTTAGAAGAACTGGAGATGGGAGATATTATATACAGGGCATTGAGTGATGGAGATGTTGTGTTGATAAATAGGCCTCCATCAATACATCAACACTCCCTAATAGCTTTAACCGCAAAGGTCCTTCCAGTAAACTCTGTAGTGTCGATAAATCCACTGTGTTGCTCTCCTTTTCGTGGTGATTTTGATGGTGATTGCCTTCATGGTTATGTTCCTCAATCAGTGGATACCAGAGTCGAGCTTACAGAGCTTGTTGCTTTAGATAAGCAATTAGTTAATGGGCAGAGTGGTTCCAACCTTCTTTCTCTCAGTCAAGATAGTTTAACAGCTTGCTATCTCATCATGGAAGATGGCACCCTGATGAATAAGTTTCAAATGCAACAACTGAAAATGCTTTGTCCCCCTGAACTGCCGTCACCAGCGATAATTAAAGATCCTTCTGGTACCTCCGTTTCTTGGACGGCAAAGCAGATAATCAGCATGTTTCTACCTGCAGATTTTAATTATGCTTTTCCTTCAAATGGCGTGCATATTAGCAATGGAGAGCTTCTAGCTTCTTCTGAAGGTTCTTCTTGGCTGCGAGACACCAGTGGGAATCTTTTCCAACATCTCATCGAACATTGCCCTGGTCAGATACTTGACATCTTCTTTACTGCTCAGGGAGTTCTATGTGAGTGGTTGTCAATGAGGGGCTTGAGTGTTTCTCTCTCAGACTTGTATCTCGCGTCGGATACATGTTCAAGGAAAAACTTGGTGGAGGAAATCTTCTATGGGTTACAAGAAGCAGAGGAGGCATGTACTATCAGACAGTTGATGCTTGATTCTTGTCAGGAATTTCTTATGGGCTATGCTGAAGAGACAAAAAGTCCTTTGACTTTTGGTTCAGAACATTTTTGTTATCAGAAGCAGAAATCTGCTGCACTTAGTCAGGTCACGGTAGATGCTTTCAAAGAAGGTTTCCGGGATGTCCAAAATTTAGCATACAAATATGCCCGTGAGGACAACTCGTTGCTGGCTATGTTTAAGGCTGGAAGCAAGGGTAATATGCTGAAATTAGTTCAGCATGGTATGTGCCTTGGTTTGCAGCACTCACTGGTTCCCTTATCATTCAAGTTTCCGAACCAGCTCTCATGTGATGCATGGAATGATCAAAAAGCACGTGGTATTGTTCAGGAGGTTGGAAGAGCTTTTGAATCCATCGAGTCATATATCCCATCCGCTGTCGTTAAAAGTTCGTTCTTGACTGGGCTGAATCCAGTCGAATGCTTTGTTCATTCAGTCACGAGTCGAGATGGTTCTTTTAGTAATAATGCGGAACTTCCTGGGACTCTGAATCGAAAGCTCATGTATTTCATGCGTGATTTGCATACTGCTTATGACGGAACAGTTAGAAACGCATATGGGAACCAGCTGGTTCAGTTCTCCTATGATATTGACAAGGGCACATCTACTGCAGATAGCACCACAAACAGCTCATATGCAAAGAGTGCTATTTTTTGTGGTGGCGGCCAACCTGTTGGTGCATTGTCTGCTTGTGCAATATCTGAAGCTGCATACAGTGCTTTAGATCAGCCAGTTAGTCTACTCGAAACTTCTCCTCTGCTAAATCTGAAGAACATTTTGGAGTGTGGTTCAAAGAAAAGCAGTGCTAAGCAAACTGTGTCATTATTTTTATCTGAGAAGCTTGGAAGACATAGGCATGGTTTCGAGTATGGAGCGCTAGAAGTTAAAAATCATCTAGAGGGACTCACATTTTCAGACATCGTTTCTACTGTCATGATAATGTTCTCCTCACAAACTGGCAGCAAGATGCATTTTAGTCCCTGGGTTTCTCACTTTCAGATATCTAAGGAAATTGTACGAAGAAGAAGGCTGAATGTGCGATCTATTATAGAGGCACTTTATGCAAGATGCAACTCCGCTGGAGCGGAGTTGAAAATTATCCTTTGGAATTTGAAGATCACAAGAAATAAGGAATGTGATTGCTGCAGCACACCTAATGATACATTTTGCATCAGTGTCACACTGACTGAAAAGCACGGCAATTCTTCTGAAGAACTGGATAAAACTCGAGTCTTGGTGATACCATTCTTGCTTAAAACAGTAGTTAAAGGTTTTCTAGAGATAAAAAAGGTGGATATTATATGGAATGATCGACCTGGTTCATCTGGTGAACTCTACTTGAGAGTTTCTATGTCAGGAAAGACCAGAAGGGGGACTACCTTTTGGAATATGCTTATGGACGATTGTCTTCCAGTTATGGATATGATTGATTGGTCACGAAGTCATCCAGATAACGTCGATGACTTCTGCACTGCCTATGGAATAGATGTTGGGTGGAAACATTTCCTTAATAAGTTGGAGTCTGCAACCGTAGATATTGGAAAGACAATTCTTCCTCAGCATTTGCTTCTTGCAGCAGATTGTCTGTCAGCCACCGGCGAGTTTGTCAGCTTAAATGCAAAAGGCATTGCTCAACAATTGGAACATGCATCTGTCACAGCCCCTTTTATGCAAGCTTGCTTTTCTGTTAACTCCTGGTCCTTGCTTGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000418 GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010495 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901698 GO:1901699 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1231

Amino Acids

137.03

Weight (kDa)

6.45

Isoelectric Point (pI)

43.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	43 - 104	3.6e-07	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	305 - 464	2.8e-35	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	469 - 618	2.8e-20	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	687 - 742	3.1e-08	RNA polymerase Rpb1, domain 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002992)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63020 AT1G63020 AT1G63020 AT1G63020 AT1G63020
fragaria_vesca	FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400
malus_domestica	MD04G1068800.v1.1 MD06G1069300.v1.1 MD06G1069400.v1.1 MD06G1069500.v1.1 MD06G1069800.v1.1
prunus_persica	Prupe.5G089300_v2.0.a1
pyrus_communis	pycom04g06250
rosa_chinensis	RchiOBHm_Chr7g0199481
rosa_laevigata	RLG00000003790
rosa_multiflora	Rmu_co8411067.1_g000001 Rmu_sc0003890.1_g000001
rosa_roxburghii	Rroxscaffold_3G00256700
rosa_rugosa	Rorug07G0054300 Rorug07G0054400 Rorug07G0054500 Rorug07G0054500
rosa_samantha	Rh7AG179300 Rh7BG182800 Rh7CG188100 Rh7DG182200
rosa_wichuraiana	Rw7G015760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1636
Acc65I	GGTACC	2 cut(s)	182, 1580
AccB1I	GGYRCC	3 cut(s)	182, 1490, 1580
AccBSI	CCGCTC	1 cut(s)	3065
AccI	GTMKAC	1 cut(s)	2719
AccII	CGCG	1 cut(s)	1862
AciI	CCGC	7 cut(s)	991, 1251, 2355, 2450, 2643, 3057, 3065
AclWI	GGATC	5 cut(s)	128, 488, 572, 950, 1565
AcoI	YGGCCR	2 cut(s)	1078, 2644
AcsI	RAATTY	4 cut(s)	795, 1970, 2110, 3088
AcuI	CTGAAG	6 cut(s)	1028, 1719, 2010, 2703, 2768, 3210
AcvI	CACGTG	1 cut(s)	2297
AfaI	GTAC	6 cut(s)	57, 184, 433, 1582, 1936, 2997
AfeI	AGCGCT	1 cut(s)	2856
AfiI	CCNNNNNNNGG	5 cut(s)	143, 1313, 1593, 1658, 3351
AflIII	ACRYGT	2 cut(s)	1102, 1871
AhdI	GACNNNNNGTC	1 cut(s)	2001
AhlI	ACTAGT	2 cut(s)	28, 736
AjnI	CCWGG	7 cut(s)	123, 549, 1762, 2458, 2960, 3295, 3674
AjuI	GAANNNNNNNTTGG	4 cut(s)	1026, 1058, 2741, 2773
Alw26I	GTCTC	2 cut(s)	1713, 1988
AlwI	GGATC	5 cut(s)	128, 488, 572, 950, 1565
Ama87I	CYCGRG	2 cut(s)	75, 3207
Aor51HI	AGCGCT	1 cut(s)	2856
AoxI	GGCC	3 cut(s)	1078, 1211, 2644
ApoI	RAATTY	4 cut(s)	795, 1970, 2110, 3088
AseI	ATTAAT	1 cut(s)	107
Asp700I	GAANNNNTTC	2 cut(s)	1736, 3482
Asp718I	GGTACC	2 cut(s)	182, 1580
AspLEI	GCGC	1 cut(s)	2857
AspS9I	GGNCC	3 cut(s)	219, 1258, 3678
AsuC2I	CCSGG	2 cut(s)	296, 2099
AsuHPI	GGTGA	7 cut(s)	965, 1106, 1328, 1340, 1545, 3229, 3320
AsuII	TTCGAA	1 cut(s)	937
AvaI	CYCGRG	2 cut(s)	75, 3207
AvaII	GGWCC	3 cut(s)	219, 1258, 3678
BaeGI	GKGCMC	1 cut(s)	2582
BaeI	ACNNNNGTAYC	4 cut(s)	1356, 1389, 1572, 1605
BalI	TGGCCA	1 cut(s)	1080
BanI	GGYRCC	3 cut(s)	182, 1490, 1580
BanII	GRGCYC	1 cut(s)	338
BarI	GAAGNNNNNNTAC	2 cut(s)	3340, 3372
BauI	CACGAG	2 cut(s)	774, 2419
BbrPI	CACGTG	1 cut(s)	2297
BbsI	GAAGAC	2 cut(s)	2833, 3380
BbvCI	CCTCAGC	1 cut(s)	3537
BceAI	ACGGC	3 cut(s)	1534, 1614, 3193
BcgI	CGANNNNNNTGC	2 cut(s)	101, 135
BciT130I	CCWGG	7 cut(s)	125, 551, 1764, 2460, 2962, 3297, 3676
BciVI	GTATCC	2 cut(s)	1357, 1861
BclI	TGATCA	2 cut(s)	925, 2284
BcnI	CCSGG	2 cut(s)	296, 2099
BcoDI	GTCTC	2 cut(s)	1713, 1988
BcuI	ACTAGT	2 cut(s)	28, 736
BfaI	CTAG	9 cut(s)	29, 387, 411, 720, 737, 1688, 2858, 2876, 3257
BfmI	CTRYAG	5 cut(s)	352, 1275, 1629, 2589, 3124
BfoI	RGCGCY	1 cut(s)	2858
BfuAI	ACCTGC	1 cut(s)	1636
BfuI	GTATCC	2 cut(s)	1357, 1861
BglII	AGATCT	1 cut(s)	1014
BlpI	GCTNAGC	1 cut(s)	2787
Bme1390I	CCNGG	9 cut(s)	125, 296, 551, 1764, 2099, 2460, 2962, 3297, 3676
Bme18I	GGWCC	3 cut(s)	219, 1258, 3678
BmeRI	GACNNNNNGTC	1 cut(s)	2001
BmeT110I	CYCGRG	2 cut(s)	75, 3207
BmgT120I	GGNCC	3 cut(s)	219, 1258, 3678
BmiI	GGNNCC	7 cut(s)	184, 220, 1429, 1492, 1582, 2235, 2543
BmrFI	CCNGG	9 cut(s)	125, 296, 551, 1764, 2099, 2460, 2962, 3297, 3676
BmrI	ACTGGG	2 cut(s)	60, 2391
BmsI	GCATC	7 cut(s)	1941, 2068, 2263, 2937, 3038, 3159, 3638
BmuI	ACTGGG	2 cut(s)	60, 2391
BoxI	GACNNNNGTC	1 cut(s)	3384
BpiI	GAAGAC	2 cut(s)	2833, 3380
BplI	GAGNNNNNCTC	4 cut(s)	1825, 1857, 2126, 2158
BpmI	CTGGAG	2 cut(s)	1172, 3081
Bpu10I	CCTNAGC	2 cut(s)	1794, 3537
Bpu1102I	GCTNAGC	1 cut(s)	2787
Bpu14I	TTCGAA	1 cut(s)	937
BpuEI	CTTGAG	3 cut(s)	907, 1852, 3340
BpuMI	CCSGG	2 cut(s)	296, 2099
BsaAI	YACGTR	1 cut(s)	2297
BsaBI	GATNNNNATC	1 cut(s)	576
BsaJI	CCNNGG	8 cut(s)	123, 549, 1075, 1762, 2212, 2459, 2960, 2961
BsaWI	WCCGGW	1 cut(s)	262
BsaXI	ACNNNNNCTCC	2 cut(s)	1182, 1212
Bsc4I	CCNNNNNNNGG	5 cut(s)	143, 1313, 1593, 1658, 3351
Bse118I	RCCGGY	1 cut(s)	3575
Bse3DI	GCAATG	3 cut(s)	1681, 1756, 3606
Bse8I	GATNNNNATC	1 cut(s)	576
BseBI	CCWGG	7 cut(s)	125, 551, 1764, 2460, 2962, 3297, 3676
BseDI	CCNNGG	8 cut(s)	123, 549, 1075, 1762, 2212, 2459, 2960, 2961
BseGI	GGATG	4 cut(s)	247, 2107, 2351, 3424
BseJI	GATNNNNATC	1 cut(s)	576
BseLI	CCNNNNNNNGG	5 cut(s)	143, 1313, 1593, 1658, 3351
BseMI	GCAATG	3 cut(s)	1681, 1756, 3606
BseMII	CTCAG	7 cut(s)	962, 1011, 1459, 1808, 1860, 2805, 3551
BseRI	GAGGAG	3 cut(s)	1940, 2724, 2917
BseSI	GKGCMC	1 cut(s)	2582
BseYI	CCCAGC	1 cut(s)	766
BsgI	GTGCAG	2 cut(s)	2040, 3433
Bsh1236I	CGCG	1 cut(s)	1862
BshFI	GGCC	3 cut(s)	1080, 1213, 2646
BshNI	GGYRCC	3 cut(s)	182, 1490, 1580
BsiHKCI	CYCGRG	2 cut(s)	75, 3207
BsiSI	CCGG	4 cut(s)	263, 296, 2098, 3576
BslFI	GGGAC	7 cut(s)	159, 491, 1521, 2476, 2895, 2943, 3367
BslI	CCNNNNNNNGG	5 cut(s)	143, 1313, 1593, 1658, 3351
BsmAI	GTCTC	2 cut(s)	1713, 1988
BsmFI	GGGAC	7 cut(s)	159, 491, 1521, 2476, 2895, 2943, 3367
BsmI	GAATGC	1 cut(s)	2405
BsnI	GGCC	3 cut(s)	1080, 1213, 2646
BsoBI	CYCGRG	2 cut(s)	75, 3207
Bsp119I	TTCGAA	1 cut(s)	937
Bsp1286I	GDGCHC	2 cut(s)	338, 2582
Bsp1720I	GCTNAGC	1 cut(s)	2787
BspACI	CCGC	7 cut(s)	991, 1251, 2355, 2450, 2643, 3057, 3065
BspANI	GGCC	3 cut(s)	1080, 1213, 2646
BspCNI	CTCAG	7 cut(s)	963, 1012, 1458, 1807, 1859, 2806, 3550
BspFNI	CGCG	1 cut(s)	1862
BspHI	TCATGA	1 cut(s)	2913
BspLI	GGNNCC	7 cut(s)	184, 220, 1429, 1492, 1582, 2235, 2543
BspMAI	CTGCAG	4 cut(s)	356, 1633, 2593, 3128
BspMI	ACCTGC	1 cut(s)	1636
BspPI	GGATC	5 cut(s)	128, 488, 572, 950, 1565
BspQI	GCTCTTC	1 cut(s)	2314
BspT104I	TTCGAA	1 cut(s)	937
BspT107I	GGYRCC	3 cut(s)	182, 1490, 1580
BsrBI	CCGCTC	1 cut(s)	3065
BsrDI	GCAATG	3 cut(s)	1681, 1756, 3606
BsrFI	RCCGGY	1 cut(s)	3575
BssAI	RCCGGY	1 cut(s)	3575
BssECI	CCNNGG	8 cut(s)	123, 549, 1075, 1762, 2212, 2459, 2960, 2961
BssSI	CACGAG	2 cut(s)	774, 2419
BssT1I	CCWWGG	2 cut(s)	1075, 2212
Bst2BI	CACGAG	2 cut(s)	774, 2419
Bst2UI	CCWGG	7 cut(s)	125, 551, 1764, 2460, 2962, 3297, 3676
Bst6I	CTCTTC	3 cut(s)	526, 1986, 2314
BstAPI	GCANNNNNTGC	5 cut(s)	360, 2624, 2666, 2696, 3041
BstBAI	YACGTR	1 cut(s)	2297
BstBI	TTCGAA	1 cut(s)	937
BstC8I	GCNNGC	7 cut(s)	43, 1067, 1468, 1664, 2151, 3654, 3658
BstF5I	GGATG	4 cut(s)	247, 2107, 2351, 3424
BstFNI	CGCG	1 cut(s)	1862
BstH2I	RGCGCY	1 cut(s)	2858
BstHHI	GCGC	1 cut(s)	2857
BstMAI	GTCTC	2 cut(s)	1713, 1988
BstNI	CCWGG	7 cut(s)	125, 551, 1764, 2460, 2962, 3297, 3676
BstNSI	RCATGY	6 cut(s)	303, 1106, 1621, 1875, 1935, 3629
BstPAI	GACNNNNGTC	1 cut(s)	3384
BstSCI	CCNGG	9 cut(s)	123, 294, 549, 1762, 2097, 2458, 2960, 3295, 3674
BstSFI	CTRYAG	5 cut(s)	352, 1275, 1629, 2589, 3124
BstSLI	GKGCMC	1 cut(s)	2582
BstUI	CGCG	1 cut(s)	1862
BstV2I	GAAGAC	2 cut(s)	2833, 3380
BstX2I	RGATCY	3 cut(s)	493, 1014, 1570
BstXI	CCANNNNNNTGG	1 cut(s)	2654
BstYI	RGATCY	3 cut(s)	493, 1014, 1570
BsuI	GTATCC	2 cut(s)	1357, 1861
BsuRI	GGCC	3 cut(s)	1080, 1213, 2646
BtsCI	GGATG	4 cut(s)	247, 2107, 2351, 3424
BtsI	GCAGTG	3 cut(s)	753, 2788, 3450
BveI	ACCTGC	1 cut(s)	1636
Cac8I	GCNNGC	7 cut(s)	43, 1067, 1468, 1664, 2151, 3654, 3658
CciI	TCATGA	1 cut(s)	2913
CfoI	GCGC	1 cut(s)	2857
Cfr10I	RCCGGY	1 cut(s)	3575
Cfr13I	GGNCC	3 cut(s)	219, 1258, 3678
CseI	GACGC	1 cut(s)	1851
CsiI	ACCWGGT	1 cut(s)	3295
Csp6I	GTAC	6 cut(s)	56, 183, 432, 1581, 1935, 2996
CviQI	GTAC	6 cut(s)	56, 183, 432, 1581, 1935, 2996
DraI	TTTAAA	1 cut(s)	282
DriI	GACNNNNNGTC	1 cut(s)	2001
EaeI	YGGCCR	2 cut(s)	1078, 2644
Eam1104I	CTCTTC	3 cut(s)	526, 1986, 2314
Eam1105I	GACNNNNNGTC	1 cut(s)	2001
EarI	CTCTTC	3 cut(s)	526, 1986, 2314
Eco130I	CCWWGG	2 cut(s)	1075, 2212
Eco147I	AGGCCT	1 cut(s)	1213
Eco24I	GRGCYC	1 cut(s)	338
Eco32I	GATATC	1 cut(s)	2982
Eco47I	GGWCC	3 cut(s)	219, 1258, 3678
Eco47III	AGCGCT	1 cut(s)	2856
Eco57I	CTGAAG	6 cut(s)	1028, 1719, 2010, 2703, 2768, 3210
Eco72I	CACGTG	1 cut(s)	2297
Eco88I	CYCGRG	2 cut(s)	75, 3207
EcoO109I	RGGNCCY	1 cut(s)	1258
EcoRII	CCWGG	7 cut(s)	123, 549, 1762, 2458, 2960, 3295, 3674
EcoRV	GATATC	1 cut(s)	2982
EcoT14I	CCWWGG	2 cut(s)	1075, 2212
EcoT22I	ATGCAT	3 cut(s)	2278, 2952, 3631
EcoT38I	GRGCYC	1 cut(s)	338
ErhI	CCWWGG	2 cut(s)	1075, 2212
FalI	AAGNNNNNCTT	2 cut(s)	258, 290
FaqI	GGGAC	7 cut(s)	159, 491, 1521, 2476, 2895, 2943, 3367
FauI	CCCGC	1 cut(s)	998
FauNDI	CATATG	2 cut(s)	2536, 2614
FbaI	TGATCA	2 cut(s)	925, 2284
FblI	GTMKAC	1 cut(s)	2719
FokI	GGATG	4 cut(s)	234, 2114, 2338, 3411
FriOI	GRGCYC	1 cut(s)	338
FspBI	CTAG	9 cut(s)	29, 387, 411, 720, 737, 1688, 2858, 2876, 3257
GlaI	GCGC	1 cut(s)	2856
GsaI	CCCAGC	1 cut(s)	770
GsuI	CTGGAG	2 cut(s)	1172, 3081
HaeII	RGCGCY	1 cut(s)	2858
HaeIII	GGCC	3 cut(s)	1080, 1213, 2646
HapII	CCGG	4 cut(s)	263, 296, 2098, 3576
HgaI	GACGC	1 cut(s)	1851
HhaI	GCGC	1 cut(s)	2857
Hin6I	GCGC	1 cut(s)	2855
HinP1I	GCGC	1 cut(s)	2855
HincII	GTYRAC	1 cut(s)	3670
HindII	GTYRAC	1 cut(s)	3670
HindIII	AAGCTT	2 cut(s)	2819, 3654
HpaI	GTTAAC	1 cut(s)	3670
HpaII	CCGG	4 cut(s)	263, 296, 2098, 3576
HphI	GGTGA	7 cut(s)	965, 1106, 1328, 1340, 1545, 3229, 3320
Hpy166II	GTNNAC	4 cut(s)	1270, 2720, 3311, 3670
Hpy8I	GTNNAC	4 cut(s)	1270, 2720, 3311, 3670
Hpy99I	CGWCG	2 cut(s)	1867, 3440
HpyCH4IV	ACGT	2 cut(s)	2296, 3435
HpySE526I	ACGT	2 cut(s)	2296, 3435
HspAI	GCGC	1 cut(s)	2855
KpnI	GGTACC	2 cut(s)	186, 1584
Ksp22I	TGATCA	2 cut(s)	925, 2284
KspAI	GTTAAC	1 cut(s)	3670
LguI	GCTCTTC	1 cut(s)	2314
LmnI	GCTCC	2 cut(s)	2852, 3062
LweI	GCATC	7 cut(s)	1941, 2068, 2263, 2937, 3038, 3159, 3638
MabI	ACCWGGT	1 cut(s)	3295
MaeI	CTAG	9 cut(s)	29, 387, 411, 720, 737, 1688, 2858, 2876, 3257
MaeII	ACGT	2 cut(s)	2296, 3435
MbiI	CCGCTC	1 cut(s)	3065
MfeI	CAATTG	1 cut(s)	3617
MflI	RGATCY	3 cut(s)	493, 1014, 1570
MhlI	GDGCHC	2 cut(s)	338, 2582
MlsI	TGGCCA	1 cut(s)	1080
MluNI	TGGCCA	1 cut(s)	1080
MlyI	GAGTC	7 cut(s)	1380, 2348, 2431, 2458, 2877, 3219, 3509
MmeI	TCCRAC	8 cut(s)	182, 433, 629, 1455, 1765, 1845, 2296, 3477
Mox20I	TGGCCA	1 cut(s)	1080
Mph1103I	ATGCAT	3 cut(s)	2278, 2952, 3631
MroXI	GAANNNNTTC	2 cut(s)	1736, 3482
MscI	TGGCCA	1 cut(s)	1080
MslI	CAYNNNNRTG	5 cut(s)	63, 1344, 1496, 2835, 2918
Msp20I	TGGCCA	1 cut(s)	1080
MspA1I	CMGCKG	4 cut(s)	119, 2357, 2548, 3059
MspI	CCGG	4 cut(s)	263, 296, 2098, 3576
MspR9I	CCNGG	9 cut(s)	125, 296, 551, 1764, 2099, 2460, 2962, 3297, 3676
MunI	CAATTG	1 cut(s)	3617
Mva1269I	GAATGC	1 cut(s)	2405
MvaI	CCWGG	7 cut(s)	125, 551, 1764, 2460, 2962, 3297, 3676
MvnI	CGCG	1 cut(s)	1862
NciI	CCSGG	2 cut(s)	296, 2099
NdeI	CATATG	2 cut(s)	2536, 2614
NlaIV	GGNNCC	7 cut(s)	184, 220, 1429, 1492, 1582, 2235, 2543
NmuCI	GTSAC	9 cut(s)	640, 661, 1094, 1551, 2068, 2416, 3157, 3414, 3634
NsiI	ATGCAT	3 cut(s)	2278, 2952, 3631
NspI	RCATGY	6 cut(s)	303, 1106, 1621, 1875, 1935, 3629
NspV	TTCGAA	1 cut(s)	937
PaeR7I	CTCGAG	1 cut(s)	3207
PagI	TCATGA	1 cut(s)	2913
PasI	CCCWGGG	1 cut(s)	2961
PceI	AGGCCT	1 cut(s)	1213
PciI	ACATGT	2 cut(s)	1102, 1871
PciSI	GCTCTTC	1 cut(s)	2314
PctI	GAATGC	1 cut(s)	2405
PdmI	GAANNNNTTC	2 cut(s)	1736, 3482
PfeI	GAWTC	9 cut(s)	163, 830, 863, 1024, 1732, 1958, 2330, 2389, 2470
PfoI	TCCNGGA	1 cut(s)	2097
PleI	GAGTC	7 cut(s)	1379, 2347, 2430, 2458, 2877, 3218, 3508
PmaCI	CACGTG	1 cut(s)	2297
PmlI	CACGTG	1 cut(s)	2297
PpsI	GAGTC	7 cut(s)	1379, 2347, 2430, 2458, 2877, 3218, 3508
Ppu21I	YACGTR	1 cut(s)	2297
PpuMI	RGGWCCY	1 cut(s)	1258
PscI	ACATGT	2 cut(s)	1102, 1871
PshAI	GACNNNNGTC	1 cut(s)	3384
PshBI	ATTAAT	1 cut(s)	107
Psp5II	RGGWCCY	1 cut(s)	1258
Psp6I	CCWGG	7 cut(s)	123, 549, 1762, 2458, 2960, 3295, 3674
PspCI	CACGTG	1 cut(s)	2297
PspFI	CCCAGC	1 cut(s)	766
PspGI	CCWGG	7 cut(s)	123, 549, 1762, 2458, 2960, 3295, 3674
PspN4I	GGNNCC	7 cut(s)	184, 220, 1429, 1492, 1582, 2235, 2543
PspPI	GGNCC	3 cut(s)	219, 1258, 3678
PspPPI	RGGWCCY	1 cut(s)	1258
PspXI	VCTCGAGB	1 cut(s)	3207
PstI	CTGCAG	4 cut(s)	356, 1633, 2593, 3128
PsuI	RGATCY	3 cut(s)	493, 1014, 1570
PvuII	CAGCTG	2 cut(s)	119, 2548
RsaI	GTAC	6 cut(s)	57, 184, 433, 1582, 1936, 2997
RsaNI	GTAC	6 cut(s)	56, 183, 432, 1581, 1935, 2996
RseI	CAYNNNNRTG	5 cut(s)	63, 1344, 1496, 2835, 2918
SapI	GCTCTTC	1 cut(s)	2314
Sau96I	GGNCC	3 cut(s)	219, 1258, 3678
SchI	GAGTC	7 cut(s)	1380, 2348, 2431, 2458, 2877, 3219, 3509
ScrFI	CCNGG	9 cut(s)	125, 296, 551, 1764, 2099, 2460, 2962, 3297, 3676
SduI	GDGCHC	2 cut(s)	338, 2582
SexAI	ACCWGGT	1 cut(s)	3295
SfaNI	GCATC	7 cut(s)	1941, 2068, 2263, 2937, 3038, 3159, 3638
SfcI	CTRYAG	5 cut(s)	352, 1275, 1629, 2589, 3124
Sfr274I	CTCGAG	1 cut(s)	3207
SfuI	TTCGAA	1 cut(s)	937
SgrAI	CRCCGGYG	1 cut(s)	3575
SinI	GGWCC	3 cut(s)	219, 1258, 3678
SlaI	CTCGAG	1 cut(s)	3207
SmiMI	CAYNNNNRTG	5 cut(s)	63, 1344, 1496, 2835, 2918
SmlI	CTYRAG	4 cut(s)	886, 1831, 3207, 3319
SmoI	CTYRAG	4 cut(s)	886, 1831, 3207, 3319
SpeI	ACTAGT	2 cut(s)	28, 736
SseBI	AGGCCT	1 cut(s)	1213
SsiI	CCGC	7 cut(s)	991, 1251, 2355, 2450, 2643, 3057, 3065
SspMI	CTAG	9 cut(s)	29, 387, 411, 720, 737, 1688, 2858, 2876, 3257
StuI	AGGCCT	1 cut(s)	1213
StyD4I	CCNGG	9 cut(s)	123, 294, 549, 1762, 2097, 2458, 2960, 3295, 3674
StyI	CCWWGG	2 cut(s)	1075, 2212
TaiI	ACGT	2 cut(s)	2299, 3438
TatI	WGTACW	2 cut(s)	431, 1934
TauI	GCSGC	1 cut(s)	2646
TfiI	GAWTC	9 cut(s)	163, 830, 863, 1024, 1732, 1958, 2330, 2389, 2470
TseFI	GTSAC	9 cut(s)	640, 661, 1094, 1551, 2068, 2416, 3157, 3414, 3634
Tsp45I	GTSAC	9 cut(s)	640, 661, 1094, 1551, 2068, 2416, 3157, 3414, 3634
TspGWI	ACGGA	5 cut(s)	101, 358, 858, 1576, 2532
VpaK11BI	GGWCC	3 cut(s)	219, 1258, 3678
VspI	ATTAAT	1 cut(s)	107
XapI	RAATTY	4 cut(s)	795, 1970, 2110, 3088
XbaI	TCTAGA	3 cut(s)	410, 2875, 3256
XceI	RCATGY	6 cut(s)	303, 1106, 1621, 1875, 1935, 3629
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1073
XhoI	CTCGAG	1 cut(s)	3207
XmiI	GTMKAC	1 cut(s)	2719
XmnI	GAANNNNTTC	2 cut(s)	1736, 3482
XspI	CTAG	9 cut(s)	29, 387, 411, 720, 737, 1688, 2858, 2876, 3257
Zsp2I	ATGCAT	3 cut(s)	2278, 2952, 3631

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.