Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0002992

Physical Location & Seq

Genomic Coordinates Forward (+)

14574843 .. 14585741

Gene Structure 10899 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG182800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4356 bp

ATGGAAGATGAATTGTATGATGAAGAACTAGTGCCATGTGCTTGCATAACTGGGGTACATCTAAATGTTTCTACTCGGGAAGATACGGAGAAAATATCAACATTATTAATCGAGGCAGCTGCCCAGGTTAATGATCCAAAGTTGGGACTTCCAAATCCAACGAATCAGTGTTCTACTTGTGGTACCCAAGATATAAAATCCTGTGATGGTCATTTTGGGTCCATCAAGTTTCCATGCACGATACTCCATCCATACTATCTTCCGGAAGTTGCTCTTATTTTAAACAAGATTTGCCCGGCATGTAAAACCATCAGGCAAGGTGTAAGGGTCAAGGGCTCCGAACGGAGGGGCTGCAGATATTGCTTTGGAGGTTCATCGGATTACCCTAGAATGAAGTTCAAAGTTTCTTCTAGAGAGCTTTTCAGAAGAAGTACAATAATAGTAGAAGCAGTTGGAAAATTGCCTCCTGATTATTGGGACTTCATTCCCAAAGATCCCCAACATGATGAAACACACACAAAACCAAATAGAAGAGTTCTATCTCATGCCCAGGTTCATTTTTTGTTGAATGATGTTGATCCAGATTTCATCACAAAGTTTGTTCCAACAACCAGCTCACTTTCTTTGGACTGTTTCCTTGTCACGCCAAATTGTCATCGTGTGACAGAAGTTATGTATTCATTTAATAATGGGCAGTCACTGATGTTTGATGATCGGACTAGAGCTTACAGGAAACTAGTGGATTTCAGAGGCACAGCGAACGAGCTGGGTTCTCGTGTTTTGGACTGCCTGAAAATTTCCAAGATAAACAAAGACAAAACAGGCAGAGATTCAATTTCAGTTGAGCAACAAAAGATTAAGGATTCTCCGTCCAGAACATCTGGCTTGAGATGGATAAAAGATGTTGTTCTTGGAAAGAGAAGTGATCACTGTTTTCGAACGGTTGTCGTTGGTGATCCAAACATCAAGCTGAGTGAGATTGGCATACCCCGACATATTGCAGAGAAAATGCAGATCTCTGAAAATCTGAACCAATATAACCTTGAGAAGTTGTATGCGTTTTGCCAGCTACGCCTTGGCCATCAGGGGAGAGGTGACATACATGTTCGTAGAAATGATAGTCTGGTTAGGATAAGTAATTTAGAAGAACTGGAGATGGGAGATATTATATACAGGGCGTTGAGTGATGGAGATGTTGTGTTGATAAATAGGCCTCCATCAATACATCAACACTCCCTAATAGCTTTAACCACAAAGGTCCTTCCAGTAAACTCTGTAGTGTCGATAAATCCACTGTGTTGCTCTCCTTTTCGTGGTGATTTTGATGGTGATTGCCTTCATGGTTATGTTCCTCAATCAGTGGATACCAGAGTCGAGCTTACAGAGCTTGTTGCTTTAGATAAGCAATTAGTTAATGGGCAGAGTGGTACCAACCTTCTTTCTCTCAGTCAAGATAGTTTAACTGCTTGCTATCTCATCATGGAAGATGGCACCCTGATGAATAAGTTTCAAATGCAACAACTGAAAATGCTTTGTCCCCCTGAACTTCCATCACCAGCGATAATTAAAGATCCTTCTGGTAATTCCGTTTCTTGGACGGCAAAGCAGATAATCAGCATGTTTCTACCTGCAGATTTTGATTATGCTTTTCCTTCAAATGGCGTGCATATTAGCAATGGACAGCTTATAGCTTCTTCTGAAGGTTCTTCTTGGCTGCGAGACACCAGTGGGAATCTTTTCCAAAGTCTCATGGAACATTGCCCCGGTCAGATACTTGACATCTTCTTTACTGCTCAGGGAGTTCTATGTGAGTGGTTGTCAATGAGGGGCTTGAGTGTTTCTCTCTCAGACTTGTATCTCGCATCGGATACATGTTCAAGGAAAAACTTGATGGAGGAAATCTTCTATGGGTTACAGGAAGCAGAGGAGGCATGTACTATCAGACAGTTGATGCTTGATTCTTGTCAGGAATTTCTTATGGGCTATGCTGAAGAGACAAAAAGTCCTTTGACTTTTGGTTCAGAACATTTTTCTTATCAGAAGCAGAAATCTGCTGCACTTAGTCAGGTCACACTAGATGCTTTCAAAGAAGGTTTCCGGGATGTCCAAAATTTAGCATACAAATATGCCCGTGAGGACAACTCGTTGCTGGCTATGTTTAAGGCTGGAAGCAAGGGTAATATGCTGAAATTAGTTCAGCACGGTATGTGCCTTGGTTTGCAGCATTCACTGGTTCCCTTATCATTCAAGTTTCCGAACCAGCTCTCATGTGATGCATGGAATGATCAAAAAGCACATGGTATCGTTCAGGAGGTTGGAAGAGCTTTTGAATCCATCGAGTCATATATCCCATCCGCTGTTGTTAAAAGTTCGTTCTTGACTGGGCTGAATCCAGTCGAATGCTTTGTTCATTCAGTCACGAGTCGAGATGGTTCTTTTAGTAATAATGCGGAACTTCCTGGGACTCTGAATCGAAAGCTCATGTATTTCATGCGTGATTTGCATACTGCTTATGACGGAACAGTTAGAAACGCATATGGGAACCACCTGGTTCAGTTCTCCTATGATATTGACGAGGGCATATCTACTGCAGATAGCACCACAAACAGCTCATATGCAAAGAGTGTTATTGATTGTGGTGGCGGCCAACCTGTTGGTGCATTGTCTGCTTGCGCAATATCTGAAGCTGCATACAGTGCTTTAGATCAGCCAGTTAGTCTACTTGAAACTTCTCCTCTGCTAAATCTGAAGAACATTTTGGAGTGTGGTTCAAAGAAAAGCAGTGCTAAGCAAACTATGTCATTATTTTTATCTGAGAAGCTTCGAAGACATAGGCATGGTTTCGAGTATGGAGCGCTAGAAGTTAAAAATCATCTAGAGGGACTCGCATTTTCAGACATCGTTTCTACTGTCATGATAATGTTCTCCTCACAAACTGGCAGCAAGATGCATTTTAGTCCTTGGGTTTCTCACTTTCATATATCTAAGGAAATTGTACGAAGAAGAAGGCTGAATGTGCGATCTATTATAGAGGCACTTTATGCAAGATGCAACTCCACTCCAGCGGAGTTGAAAACTATCCTTTGGAATTTGAAGATCACAAGAAATAAGGAATGTGATTGCTGCAGCACACCTACTGATACATTTTGCATCAGTGTCACACTGACTGAAAACTGTGTCACACTGACTGAAAAGCACAACAATTCTCCTGAAGAACTGGATAAAATTCGAGTCTTGGTGATACCATTCTTGCTTAAAACAGTAGTTAAAGGTTTTCTGGAGATAAAAAAGGTGGATATTATATGGAATGATCGACCTGGTTCATCTGGTGAACTCTACTTGAGAGTTTCTATGTCAGGAAAGACCGGAAGAGGGACTACCTTTTGGAATATGCTTATGGACGATTGTCTTCCGGTCATGGATATGATTGATTGGTCACGAAGTCATCCAGATAACGTCGATGACTTCTGCACTGCCTATGGAATAGATGTTGGGTGGAAACATTTCCTTAATAAGTTGGAGTCTGCAACCGTAGATATTGGAAAGACAATTCTTCCTCAGCATTTGCTTCTTGCAGCAGATTGTCTGTCAGCCACCGGCGAGTTTGTCAGCTTAAACGCAAAAGGCATTGCTCAACAATTGGAACATGCATCTGTCACAGCCCCTTTTATGCAAGCTTGCTTTTCTACTCCTGGTCCTTGCTTGGTTAAAGCTGCTAAGGCTGGAGTGGTGGATAATCTCCAGGGGAGTTTGGACGCATTGACATGGGGAAATACTCCGTCCTTGGGAAGTGGTGGACAGTTTGATATTATATTTGCTGGGGAGGGATCTGTGTCTTCTAAGCCATTTGATGTTCATGATATGCTGAGCAGACAAATCAGTTCCAACCCTCAGAATGTATTTGAAATGTCTGATGCTCCAATTCATCTATCTGACAAATATGTTGCTCCACATGTGTACAAGTATGGTCTTTTGTGCAAGTTGAAGAGTATTACCAAATCAGTCTTAAGAGAAACTTTTACGATCCAGGATATTGATAGATTGTCTCAAACCTTGAAGAATATATTGCACAAGTATGACATCAATGAAAGCATAAATGAGATGGAGAAGAAAGCTTTGATGACGGCTTTAGAATTTCACCCTCATAGGGATCGTAAGATCGGATTAGGAATCAAAGATATAAAGGTTGGCCAGCACCCTAACCATGAGAATGCACGATGCTTCATGCTAGTACATACGGACGGGACGATTGAAGATGTTTCGTACCACAAGTGCATTATTGGTGCTTTTGATATCATAGCTCCTCATAGGGCAAAGGTCTATCGCTTGAGACATTTGAGCCGTGGTCCTCGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000418 GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010495 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0030880 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901698 GO:1901699 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3223 RNA_pol_Rpb1_1 RNA_pol_Rpb1_2 RNA_pol_Rpb1_3 RNA_pol_Rpb1_4 RNA_pol_Rpb1_5

Protein Analysis

1451

Amino Acids

161.88

Weight (kDa)

6.57

Isoelectric Point (pI)

42.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb1_1	PF04997	43 - 104	4.5e-07	RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2	PF00623	305 - 464	6.2e-35	RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3	PF04983	469 - 618	5.2e-19	RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4	PF05000	690 - 742	5.2e-08	RNA polymerase Rpb1, domain 4
DUF3223	PF11523	1353 - 1426	2.3e-25	Protein of unknown function (DUF3223)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002992)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63020 AT1G63020 AT1G63020 AT1G63020 AT1G63020
fragaria_vesca	FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400 FvH4_5g01400
malus_domestica	MD04G1068800.v1.1 MD06G1069300.v1.1 MD06G1069400.v1.1 MD06G1069500.v1.1 MD06G1069800.v1.1
prunus_persica	Prupe.5G089300_v2.0.a1
pyrus_communis	pycom04g06250
rosa_chinensis	RchiOBHm_Chr7g0199481
rosa_laevigata	RLG00000003790
rosa_multiflora	Rmu_co8411067.1_g000001 Rmu_sc0003890.1_g000001
rosa_roxburghii	Rroxscaffold_3G00256700
rosa_rugosa	Rorug07G0054300 Rorug07G0054400 Rorug07G0054500 Rorug07G0054500
rosa_samantha	Rh7AG179300 Rh7BG182800 Rh7CG188100 Rh7DG182200
rosa_wichuraiana	Rw7G015760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	2674
Acc36I	ACCTGC	1 cut(s)	1636
Acc65I	GGTACC	2 cut(s)	182, 1427
AccB1I	GGYRCC	3 cut(s)	182, 1427, 1490
AccI	GTMKAC	1 cut(s)	2719
AccIII	TCCGGA	1 cut(s)	262
AciI	CCGC	4 cut(s)	2355, 2450, 2643, 3065
AclWI	GGATC	8 cut(s)	128, 488, 572, 950, 1565, 3835, 4018, 4158
AcoI	YGGCCR	3 cut(s)	1078, 2644, 4189
AcsI	RAATTY	6 cut(s)	795, 1970, 2110, 3088, 3225, 4133
AcuI	CTGAAG	5 cut(s)	1719, 2010, 2703, 2768, 3231
AfaI	GTAC	9 cut(s)	57, 184, 433, 1429, 1936, 2997, 3959, 4233, 4265
AfeI	AGCGCT	1 cut(s)	2856
AfiI	CCNNNNNNNGG	8 cut(s)	143, 345, 1313, 1593, 1658, 3372, 3785, 4147
AflII	CTTAAG	1 cut(s)	4006
AflIII	ACRYGT	3 cut(s)	1102, 1871, 3952
AhdI	GACNNNNNGTC	1 cut(s)	2001
AhlI	ACTAGT	2 cut(s)	28, 736
AjnI	CCWGG	8 cut(s)	123, 549, 2458, 2547, 3316, 3691, 3741, 4026
AjuI	GAANNNNNNNTTGG	2 cut(s)	2741, 2773
Alw26I	GTCTC	5 cut(s)	1713, 1751, 1988, 4050, 4324
AlwI	GGATC	8 cut(s)	128, 488, 572, 950, 1565, 3835, 4018, 4158
AlwNI	CAGNNNCTG	1 cut(s)	700
Ama87I	CYCGRG	1 cut(s)	75
Aor13HI	TCCGGA	1 cut(s)	262
Aor51HI	AGCGCT	1 cut(s)	2856
AoxI	GGCC	4 cut(s)	1078, 1211, 2644, 4189
ApoI	RAATTY	6 cut(s)	795, 1970, 2110, 3088, 3225, 4133
AseI	ATTAAT	1 cut(s)	107
Asp700I	GAANNNNTTC	2 cut(s)	1736, 3503
Asp718I	GGTACC	2 cut(s)	182, 1427
AspLEI	GCGC	2 cut(s)	2675, 2857
AspS9I	GGNCC	4 cut(s)	219, 1258, 3695, 4346
AsuC2I	CCSGG	3 cut(s)	296, 1764, 2099
AsuHPI	GGTGA	8 cut(s)	965, 1106, 1328, 1340, 1545, 3250, 3341, 4130
AsuII	TTCGAA	2 cut(s)	937, 2824
AvaI	CYCGRG	1 cut(s)	75
AvaII	GGWCC	4 cut(s)	219, 1258, 3695, 4346
BaeI	ACNNNNGTAYC	2 cut(s)	1356, 1389
BalI	TGGCCA	2 cut(s)	1080, 4191
BanI	GGYRCC	3 cut(s)	182, 1427, 1490
BanII	GRGCYC	1 cut(s)	338
BarI	GAAGNNNNNNTAC	2 cut(s)	3361, 3393
BauI	CACGAG	2 cut(s)	774, 2419
BbsI	GAAGAC	3 cut(s)	2833, 3401, 3828
BbvCI	CCTCAGC	1 cut(s)	3558
BceAI	ACGGC	3 cut(s)	1614, 4140, 4326
BcgI	CGANNNNNNTGC	2 cut(s)	101, 135
BciT130I	CCWGG	8 cut(s)	125, 551, 2460, 2549, 3318, 3693, 3743, 4028
BciVI	GTATCC	2 cut(s)	1357, 1861
BclI	TGATCA	2 cut(s)	925, 2284
BcnI	CCSGG	3 cut(s)	296, 1764, 2099
BcoDI	GTCTC	5 cut(s)	1713, 1751, 1988, 4050, 4324
BcuI	ACTAGT	2 cut(s)	28, 736
BfaI	CTAG	9 cut(s)	29, 387, 411, 720, 737, 2075, 2858, 2876, 4229
BfmI	CTRYAG	5 cut(s)	352, 1275, 1629, 2589, 3124
BfoI	RGCGCY	1 cut(s)	2858
BfrI	CTTAAG	1 cut(s)	4006
BfuAI	ACCTGC	1 cut(s)	1636
BfuI	GTATCC	2 cut(s)	1357, 1861
BglII	AGATCT	1 cut(s)	1014
BlpI	GCTNAGC	2 cut(s)	2787, 3866
Bme18I	GGWCC	4 cut(s)	219, 1258, 3695, 4346
BmeRI	GACNNNNNGTC	1 cut(s)	2001
BmeT110I	CYCGRG	1 cut(s)	75
BmgT120I	GGNCC	4 cut(s)	219, 1258, 3695, 4346
BmiI	GGNNCC	7 cut(s)	184, 220, 337, 1429, 1492, 2235, 2543
BmrI	ACTGGG	2 cut(s)	60, 2391
BmuI	ACTGGG	2 cut(s)	60, 2391
BoxI	GACNNNNGTC	1 cut(s)	3405
BpiI	GAAGAC	3 cut(s)	2833, 3401, 3828
BplI	GAGNNNNNCTC	4 cut(s)	1825, 1857, 2126, 2158
BpmI	CTGGAG	5 cut(s)	1172, 3045, 3299, 3725, 3744
Bpu10I	CCTNAGC	3 cut(s)	1794, 3558, 3717
Bpu1102I	GCTNAGC	2 cut(s)	2787, 3866
Bpu14I	TTCGAA	2 cut(s)	937, 2824
BpuEI	CTTGAG	5 cut(s)	907, 1064, 1852, 3361, 4348
BpuMI	CCSGG	3 cut(s)	296, 1764, 2099
BsaBI	GATNNNNATC	1 cut(s)	576
BsaWI	WCCGGW	3 cut(s)	262, 3365, 3412
BsaXI	ACNNNNNCTCC	2 cut(s)	1182, 1212
Bsc4I	CCNNNNNNNGG	8 cut(s)	143, 345, 1313, 1593, 1658, 3372, 3785, 4147
Bse118I	RCCGGY	1 cut(s)	3596
Bse3DI	GCAATG	3 cut(s)	1681, 1756, 3627
Bse8I	GATNNNNATC	1 cut(s)	576
BseAI	TCCGGA	1 cut(s)	262
BseBI	CCWGG	8 cut(s)	125, 551, 2460, 2549, 3318, 3693, 3743, 4028
BseGI	GGATG	4 cut(s)	247, 2107, 2351, 3445
BseJI	GATNNNNATC	1 cut(s)	576
BseLI	CCNNNNNNNGG	8 cut(s)	143, 345, 1313, 1593, 1658, 3372, 3785, 4147
BseMI	GCAATG	3 cut(s)	1681, 1756, 3627
BseMII	CTCAG	8 cut(s)	962, 1459, 1808, 1860, 2805, 3572, 3857, 3905
BseRI	GAGGAG	4 cut(s)	1940, 2724, 2917, 4293
BseYI	CCCAGC	2 cut(s)	766, 3818
BsgI	GTGCAG	2 cut(s)	2040, 3454
BshFI	GGCC	4 cut(s)	1080, 1213, 2646, 4191
BshNI	GGYRCC	3 cut(s)	182, 1427, 1490
BsiHKCI	CYCGRG	1 cut(s)	75
BsiSI	CCGG	7 cut(s)	263, 296, 1764, 2098, 3366, 3413, 3597
BslFI	GGGAC	7 cut(s)	159, 491, 1521, 2476, 2895, 3388, 4258
BslI	CCNNNNNNNGG	8 cut(s)	143, 345, 1313, 1593, 1658, 3372, 3785, 4147
BsmAI	GTCTC	5 cut(s)	1713, 1751, 1988, 4050, 4324
BsmFI	GGGAC	7 cut(s)	159, 491, 1521, 2476, 2895, 3388, 4258
BsmI	GAATGC	3 cut(s)	2224, 2405, 4216
BsnI	GGCC	4 cut(s)	1080, 1213, 2646, 4191
BsoBI	CYCGRG	1 cut(s)	75
Bsp119I	TTCGAA	2 cut(s)	937, 2824
Bsp1286I	GDGCHC	1 cut(s)	338
Bsp13I	TCCGGA	1 cut(s)	262
Bsp1407I	TGTACA	1 cut(s)	3957
Bsp1720I	GCTNAGC	2 cut(s)	2787, 3866
BspACI	CCGC	4 cut(s)	2355, 2450, 2643, 3065
BspANI	GGCC	4 cut(s)	1080, 1213, 2646, 4191
BspCNI	CTCAG	8 cut(s)	963, 1458, 1807, 1859, 2806, 3571, 3858, 3904
BspEI	TCCGGA	1 cut(s)	262
BspHI	TCATGA	2 cut(s)	2913, 3856
BspLI	GGNNCC	7 cut(s)	184, 220, 337, 1429, 1492, 2235, 2543
BspMAI	CTGCAG	4 cut(s)	356, 1633, 2593, 3128
BspMI	ACCTGC	1 cut(s)	1636
BspPI	GGATC	8 cut(s)	128, 488, 572, 950, 1565, 3835, 4018, 4158
BspQI	GCTCTTC	1 cut(s)	2314
BspT104I	TTCGAA	2 cut(s)	937, 2824
BspT107I	GGYRCC	3 cut(s)	182, 1427, 1490
BspTI	CTTAAG	1 cut(s)	4006
BsrDI	GCAATG	3 cut(s)	1681, 1756, 3627
BsrFI	RCCGGY	1 cut(s)	3596
BsrGI	TGTACA	1 cut(s)	3957
BssAI	RCCGGY	1 cut(s)	3596
BssSI	CACGAG	2 cut(s)	774, 2419
BssT1I	CCWWGG	4 cut(s)	1075, 2212, 2960, 3783
Bst2BI	CACGAG	2 cut(s)	774, 2419
Bst2UI	CCWGG	8 cut(s)	125, 551, 2460, 2549, 3318, 3693, 3743, 4028
Bst6I	CTCTTC	5 cut(s)	526, 1986, 2314, 3364, 3980
BstAFI	CTTAAG	1 cut(s)	4006
BstAPI	GCANNNNNTGC	4 cut(s)	360, 2666, 2696, 3041
BstAUI	TGTACA	1 cut(s)	3957
BstBI	TTCGAA	2 cut(s)	937, 2824
BstC8I	GCNNGC	9 cut(s)	43, 1067, 1468, 1664, 2151, 2671, 3675, 3679, 4193
BstDSI	CCRYGG	1 cut(s)	4342
BstF5I	GGATG	4 cut(s)	247, 2107, 2351, 3445
BstH2I	RGCGCY	1 cut(s)	2858
BstHHI	GCGC	2 cut(s)	2675, 2857
BstMAI	GTCTC	5 cut(s)	1713, 1751, 1988, 4050, 4324
BstNI	CCWGG	8 cut(s)	125, 551, 2460, 2549, 3318, 3693, 3743, 4028
BstNSI	RCATGY	7 cut(s)	303, 1106, 1621, 1875, 1935, 3650, 3956
BstPAI	GACNNNNGTC	1 cut(s)	3405
BstSFI	CTRYAG	5 cut(s)	352, 1275, 1629, 2589, 3124
BstV2I	GAAGAC	3 cut(s)	2833, 3401, 3828
BstX2I	RGATCY	4 cut(s)	493, 1014, 1570, 3827
BstXI	CCANNNNNNTGG	1 cut(s)	2654
BstYI	RGATCY	4 cut(s)	493, 1014, 1570, 3827
BsuI	GTATCC	2 cut(s)	1357, 1861
BsuRI	GGCC	4 cut(s)	1080, 1213, 2646, 4191
BtgI	CCRYGG	1 cut(s)	4342
BtsCI	GGATG	4 cut(s)	247, 2107, 2351, 3445
BtsI	GCAGTG	2 cut(s)	2788, 3471
BveI	ACCTGC	1 cut(s)	1636
Cac8I	GCNNGC	9 cut(s)	43, 1067, 1468, 1664, 2151, 2671, 3675, 3679, 4193
CaiI	CAGNNNCTG	1 cut(s)	700
CciI	TCATGA	2 cut(s)	2913, 3856
CfoI	GCGC	2 cut(s)	2675, 2857
Cfr10I	RCCGGY	1 cut(s)	3596
Cfr13I	GGNCC	4 cut(s)	219, 1258, 3695, 4346
CseI	GACGC	1 cut(s)	3764
CsiI	ACCWGGT	2 cut(s)	2547, 3316
Csp6I	GTAC	9 cut(s)	56, 183, 432, 1428, 1935, 2996, 3958, 4232, 4264
CviQI	GTAC	9 cut(s)	56, 183, 432, 1428, 1935, 2996, 3958, 4232, 4264
DraI	TTTAAA	1 cut(s)	282
DriI	GACNNNNNGTC	1 cut(s)	2001
EaeI	YGGCCR	3 cut(s)	1078, 2644, 4189
Eam1104I	CTCTTC	5 cut(s)	526, 1986, 2314, 3364, 3980
Eam1105I	GACNNNNNGTC	1 cut(s)	2001
EarI	CTCTTC	5 cut(s)	526, 1986, 2314, 3364, 3980
Eco130I	CCWWGG	4 cut(s)	1075, 2212, 2960, 3783
Eco147I	AGGCCT	1 cut(s)	1213
Eco24I	GRGCYC	1 cut(s)	338
Eco32I	GATATC	1 cut(s)	4294
Eco47I	GGWCC	4 cut(s)	219, 1258, 3695, 4346
Eco47III	AGCGCT	1 cut(s)	2856
Eco57I	CTGAAG	5 cut(s)	1719, 2010, 2703, 2768, 3231
Eco88I	CYCGRG	1 cut(s)	75
EcoO109I	RGGNCCY	1 cut(s)	1258
EcoRII	CCWGG	8 cut(s)	123, 549, 2458, 2547, 3316, 3691, 3741, 4026
EcoRV	GATATC	1 cut(s)	4294
EcoT14I	CCWWGG	4 cut(s)	1075, 2212, 2960, 3783
EcoT22I	ATGCAT	3 cut(s)	2278, 2952, 3652
EcoT38I	GRGCYC	1 cut(s)	338
ErhI	CCWWGG	4 cut(s)	1075, 2212, 2960, 3783
FalI	AAGNNNNNCTT	4 cut(s)	258, 290, 4000, 4032
FaqI	GGGAC	7 cut(s)	159, 491, 1521, 2476, 2895, 3388, 4258
FauNDI	CATATG	2 cut(s)	2536, 2614
FbaI	TGATCA	2 cut(s)	925, 2284
FblI	GTMKAC	1 cut(s)	2719
FokI	GGATG	4 cut(s)	234, 2114, 2338, 3432
FriOI	GRGCYC	1 cut(s)	338
FspBI	CTAG	9 cut(s)	29, 387, 411, 720, 737, 2075, 2858, 2876, 4229
FspI	TGCGCA	1 cut(s)	2674
GlaI	GCGC	2 cut(s)	2674, 2856
GsaI	CCCAGC	2 cut(s)	770, 3822
GsuI	CTGGAG	5 cut(s)	1172, 3045, 3299, 3725, 3744
HaeII	RGCGCY	1 cut(s)	2858
HaeIII	GGCC	4 cut(s)	1080, 1213, 2646, 4191
HapII	CCGG	7 cut(s)	263, 296, 1764, 2098, 3366, 3413, 3597
HgaI	GACGC	1 cut(s)	3764
HhaI	GCGC	2 cut(s)	2675, 2857
Hin6I	GCGC	2 cut(s)	2673, 2855
HinP1I	GCGC	2 cut(s)	2673, 2855
HindIII	AAGCTT	3 cut(s)	2819, 3675, 4113
HpaII	CCGG	7 cut(s)	263, 296, 1764, 2098, 3366, 3413, 3597
HphI	GGTGA	8 cut(s)	965, 1106, 1328, 1340, 1545, 3250, 3341, 4130
Hpy166II	GTNNAC	5 cut(s)	1270, 2720, 3332, 3797, 3958
Hpy8I	GTNNAC	5 cut(s)	1270, 2720, 3332, 3797, 3958
Hpy99I	CGWCG	1 cut(s)	3461
HpyAV	CCTTC	8 cut(s)	1271, 1346, 1445, 1584, 1662, 1694, 2084, 3000
HpyCH4IV	ACGT	1 cut(s)	3456
HpySE526I	ACGT	1 cut(s)	3456
HspAI	GCGC	2 cut(s)	2673, 2855
Kpn2I	TCCGGA	1 cut(s)	262
KpnI	GGTACC	2 cut(s)	186, 1431
Ksp22I	TGATCA	2 cut(s)	925, 2284
LguI	GCTCTTC	1 cut(s)	2314
LmnI	GCTCC	5 cut(s)	341, 2852, 3922, 3952, 4306
MabI	ACCWGGT	2 cut(s)	2547, 3316
MaeI	CTAG	9 cut(s)	29, 387, 411, 720, 737, 2075, 2858, 2876, 4229
MaeII	ACGT	1 cut(s)	3456
MfeI	CAATTG	1 cut(s)	3638
MflI	RGATCY	4 cut(s)	493, 1014, 1570, 3827
MhlI	GDGCHC	1 cut(s)	338
MlsI	TGGCCA	2 cut(s)	1080, 4191
MluNI	TGGCCA	2 cut(s)	1080, 4191
MlyI	GAGTC	7 cut(s)	1380, 2348, 2431, 2458, 2877, 3240, 3530
MmeI	TCCRAC	6 cut(s)	182, 433, 629, 2296, 3498, 3909
Mox20I	TGGCCA	2 cut(s)	1080, 4191
Mph1103I	ATGCAT	3 cut(s)	2278, 2952, 3652
MroI	TCCGGA	1 cut(s)	262
MroXI	GAANNNNTTC	2 cut(s)	1736, 3503
MscI	TGGCCA	2 cut(s)	1080, 4191
MslI	CAYNNNNRTG	9 cut(s)	63, 1344, 1496, 2835, 2918, 3422, 3763, 4074, 4209
Msp20I	TGGCCA	2 cut(s)	1080, 4191
MspA1I	CMGCKG	3 cut(s)	119, 2357, 3065
MspCI	CTTAAG	1 cut(s)	4006
MspI	CCGG	7 cut(s)	263, 296, 1764, 2098, 3366, 3413, 3597
MunI	CAATTG	1 cut(s)	3638
Mva1269I	GAATGC	3 cut(s)	2224, 2405, 4216
MvaI	CCWGG	8 cut(s)	125, 551, 2460, 2549, 3318, 3693, 3743, 4028
NciI	CCSGG	3 cut(s)	296, 1764, 2099
NdeI	CATATG	2 cut(s)	2536, 2614
NlaIV	GGNNCC	7 cut(s)	184, 220, 337, 1429, 1492, 2235, 2543
NsbI	TGCGCA	1 cut(s)	2674
NsiI	ATGCAT	3 cut(s)	2278, 2952, 3652
NspI	RCATGY	7 cut(s)	303, 1106, 1621, 1875, 1935, 3650, 3956
NspV	TTCGAA	2 cut(s)	937, 2824
PagI	TCATGA	2 cut(s)	2913, 3856
PceI	AGGCCT	1 cut(s)	1213
PciI	ACATGT	3 cut(s)	1102, 1871, 3952
PciSI	GCTCTTC	1 cut(s)	2314
PcsI	WCGNNNNNNNCGW	1 cut(s)	4244
PctI	GAATGC	3 cut(s)	2224, 2405, 4216
PdmI	GAANNNNTTC	2 cut(s)	1736, 3503
PfeI	GAWTC	9 cut(s)	163, 830, 863, 1732, 1958, 2330, 2389, 2470, 4170
PfoI	TCCNGGA	2 cut(s)	2097, 4026
PleI	GAGTC	7 cut(s)	1379, 2347, 2430, 2458, 2877, 3239, 3529
PpsI	GAGTC	7 cut(s)	1379, 2347, 2430, 2458, 2877, 3239, 3529
PpuMI	RGGWCCY	1 cut(s)	1258
PscI	ACATGT	3 cut(s)	1102, 1871, 3952
PshAI	GACNNNNGTC	1 cut(s)	3405
PshBI	ATTAAT	1 cut(s)	107
Psp5II	RGGWCCY	1 cut(s)	1258
Psp6I	CCWGG	8 cut(s)	123, 549, 2458, 2547, 3316, 3691, 3741, 4026
PspFI	CCCAGC	2 cut(s)	766, 3818
PspGI	CCWGG	8 cut(s)	123, 549, 2458, 2547, 3316, 3691, 3741, 4026
PspN4I	GGNNCC	7 cut(s)	184, 220, 337, 1429, 1492, 2235, 2543
PspPI	GGNCC	4 cut(s)	219, 1258, 3695, 4346
PspPPI	RGGWCCY	1 cut(s)	1258
PstI	CTGCAG	4 cut(s)	356, 1633, 2593, 3128
PstNI	CAGNNNCTG	1 cut(s)	700
PsuI	RGATCY	4 cut(s)	493, 1014, 1570, 3827
PvuII	CAGCTG	1 cut(s)	119
RsaI	GTAC	9 cut(s)	57, 184, 433, 1429, 1936, 2997, 3959, 4233, 4265
RsaNI	GTAC	9 cut(s)	56, 183, 432, 1428, 1935, 2996, 3958, 4232, 4264
RseI	CAYNNNNRTG	9 cut(s)	63, 1344, 1496, 2835, 2918, 3422, 3763, 4074, 4209
SapI	GCTCTTC	1 cut(s)	2314
Sau96I	GGNCC	4 cut(s)	219, 1258, 3695, 4346
SchI	GAGTC	7 cut(s)	1380, 2348, 2431, 2458, 2877, 3240, 3530
SduI	GDGCHC	1 cut(s)	338
SexAI	ACCWGGT	2 cut(s)	2547, 3316
SfcI	CTRYAG	5 cut(s)	352, 1275, 1629, 2589, 3124
SfuI	TTCGAA	2 cut(s)	937, 2824
SgrAI	CRCCGGYG	1 cut(s)	3596
SinI	GGWCC	4 cut(s)	219, 1258, 3695, 4346
SmiMI	CAYNNNNRTG	9 cut(s)	63, 1344, 1496, 2835, 2918, 3422, 3763, 4074, 4209
SmlI	CTYRAG	6 cut(s)	886, 1043, 1831, 3340, 4006, 4327
SmoI	CTYRAG	6 cut(s)	886, 1043, 1831, 3340, 4006, 4327
SpeI	ACTAGT	2 cut(s)	28, 736
SseBI	AGGCCT	1 cut(s)	1213
SsiI	CCGC	4 cut(s)	2355, 2450, 2643, 3065
SspMI	CTAG	9 cut(s)	29, 387, 411, 720, 737, 2075, 2858, 2876, 4229
StuI	AGGCCT	1 cut(s)	1213
StyI	CCWWGG	4 cut(s)	1075, 2212, 2960, 3783
TaiI	ACGT	1 cut(s)	3459
TatI	WGTACW	4 cut(s)	431, 1934, 3957, 4231
TauI	GCSGC	1 cut(s)	2646
TfiI	GAWTC	9 cut(s)	163, 830, 863, 1732, 1958, 2330, 2389, 2470, 4170
TspGWI	ACGGA	7 cut(s)	101, 358, 858, 1576, 2532, 3768, 4253
Vha464I	CTTAAG	1 cut(s)	4006
VpaK11BI	GGWCC	4 cut(s)	219, 1258, 3695, 4346
VspI	ATTAAT	1 cut(s)	107
XapI	RAATTY	6 cut(s)	795, 1970, 2110, 3088, 3225, 4133
XbaI	TCTAGA	2 cut(s)	410, 2875
XceI	RCATGY	7 cut(s)	303, 1106, 1621, 1875, 1935, 3650, 3956
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1073
XmiI	GTMKAC	1 cut(s)	2719
XmnI	GAANNNNTTC	2 cut(s)	1736, 3503
XspI	CTAG	9 cut(s)	29, 387, 411, 720, 737, 2075, 2858, 2876, 4229
Zsp2I	ATGCAT	3 cut(s)	2278, 2952, 3652

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.