MD04G1122000.v1.1

Glycine-rich RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008734
Physical Location & Seq
Reverse (-)
20773795 .. 20777643
3849 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1122000.v1.1.491

Sequence Viewer

Length: 687 bp
ATGGCGTTCATCTCCGCCTTCCGCCGCATCCACGGCGGATCTTCCTCCGCTCTCCATTCCCAATTCGCAGCCATTCGTCACAATTCAACTCTCACATCCCCCAGGCTGTTCATCAGCGGATTTTTTGCCGAACCTCGTAAATTTCTTGGTGTCTTTATTTCTTGTATTTTATTCTGTTTAACTGAGTGTGATTTTGGTGAGGCACAACATCTCGTGCTTCAATCTGTGTTCCTTTTTTGTTATTTTGGATTTGATTACAAAGCTTCAATCCAAGACATTCCAATTTTTCAACTGATTGATCGAAAACAAAACAAAACTAATACGGCTGCAAATGTGGTAAAGTCTTGTAAATATGCCATCTGTTTACTGAAGAACTGTCTCTCGAGATTAACAACAGACGAAAAGCTTAGAGAGGCATTTTCCCCATTTGGGCAGATTGTAGATGCGAAAGTGGTTATCGATAGGGTGTCTCAAAGATCAAAGGGTTTTGGTTTCATTACATACGCATCAGTTGAAGAAGCCGAGAAAGCTAGAGAAGGGATGAATGCCAAGTTCTTGGATGGGTGGGTTATTTTTGTCGACCCTGCCAAACCTAGGGAGCCTAGATTTCCTCCTCCTCCGCAAGCACAGCAGGACCCTTCTGAAACCGGCTTCAGATCAAACAAGACCATCGGATGGTGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

229

Amino Acids

25.62

Weight (kDa)

9.04

Isoelectric Point (pI)

52.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 127 - 188 2.2e-17 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 675
AccBSI CCGCTC 1 cut(s) 50
AccI GTMKAC 1 cut(s) 579
AciI CCGC 7 cut(s) 15, 22, 25, 36, 48, 117, 620
AclWI GGATC 1 cut(s) 46
AcsI RAATTY 1 cut(s) 140
AcuI CTGAAG 2 cut(s) 389, 637
AfiI CCNNNNNNNGG 3 cut(s) 429, 594, 675
AgsI TTSAA 5 cut(s) 87, 221, 267, 290, 515
AjnI CCWGG 1 cut(s) 101
AluBI AGCT 3 cut(s) 263, 406, 530
AluI AGCT 3 cut(s) 263, 406, 530
Alw26I GTCTC 2 cut(s) 383, 474
AlwI GGATC 1 cut(s) 46
Ama87I CYCGRG 1 cut(s) 382
ApeKI GCWGC 2 cut(s) 68, 326
ApoI RAATTY 1 cut(s) 140
AspA2I CCTAGG 1 cut(s) 593
AspS9I GGNCC 1 cut(s) 634
AsuHPI GGTGA 1 cut(s) 209
AvaI CYCGRG 1 cut(s) 382
AvaII GGWCC 1 cut(s) 634
AvrII CCTAGG 1 cut(s) 593
BauI CACGAG 1 cut(s) 212
BbvI GCAGC 2 cut(s) 80, 313
BccI CCATC 4 cut(s) 365, 554, 669, 677
BceAI ACGGC 2 cut(s) 49, 339
BciT130I CCWGG 1 cut(s) 103
BcoDI GTCTC 2 cut(s) 383, 474
BfaI CTAG 3 cut(s) 531, 594, 603
BisI GCNGC 3 cut(s) 25, 69, 327
BlnI CCTAGG 1 cut(s) 593
BlsI GCNGC 3 cut(s) 26, 70, 328
Bme1390I CCNGG 1 cut(s) 103
Bme18I GGWCC 1 cut(s) 634
BmeT110I CYCGRG 1 cut(s) 382
BmgT120I GGNCC 1 cut(s) 634
BmiI GGNNCC 2 cut(s) 600, 636
BmrFI CCNGG 1 cut(s) 103
BmsI GCATC 3 cut(s) 36, 433, 515
Bsa29I ATCGAT 1 cut(s) 459
BsaJI CCNNGG 3 cut(s) 31, 101, 593
Bsc4I CCNNNNNNNGG 3 cut(s) 429, 594, 675
Bse118I RCCGGY 1 cut(s) 647
BseBI CCWGG 1 cut(s) 103
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 3 cut(s) 31, 101, 593
BseGI GGATG 5 cut(s) 27, 95, 546, 565, 680
BseLI CCNNNNNNNGG 3 cut(s) 429, 594, 675
BseMII CTCAG 1 cut(s) 174
BseRI GAGGAG 2 cut(s) 603, 606
BseXI GCAGC 2 cut(s) 80, 313
BshVI ATCGAT 1 cut(s) 459
BsiHKCI CYCGRG 1 cut(s) 382
BsiSI CCGG 1 cut(s) 648
BslI CCNNNNNNNGG 3 cut(s) 429, 594, 675
BsmAI GTCTC 2 cut(s) 383, 474
BsmI GAATGC 1 cut(s) 550
BsoBI CYCGRG 1 cut(s) 382
Bsp143I GATC 4 cut(s) 38, 298, 476, 656
BspACI CCGC 7 cut(s) 15, 22, 25, 36, 48, 117, 620
BspCNI CTCAG 1 cut(s) 175
BspDI ATCGAT 1 cut(s) 459
BspLI GGNNCC 2 cut(s) 600, 636
BspPI GGATC 1 cut(s) 46
BsrBI CCGCTC 1 cut(s) 50
BsrFI RCCGGY 1 cut(s) 647
BssAI RCCGGY 1 cut(s) 647
BssECI CCNNGG 3 cut(s) 31, 101, 593
BssMI GATC 4 cut(s) 38, 298, 476, 656
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 1 cut(s) 593
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 1 cut(s) 103
Bst4CI ACNGT 1 cut(s) 377
BstC8I GCNNGC 1 cut(s) 624
BstDEI CTNAG 2 cut(s) 183, 407
BstDSI CCRYGG 1 cut(s) 31
BstF5I GGATG 5 cut(s) 27, 95, 546, 565, 680
BstKTI GATC 4 cut(s) 41, 301, 479, 659
BstMAI GTCTC 2 cut(s) 383, 474
BstMBI GATC 4 cut(s) 38, 298, 476, 656
BstMWI GCNNNNNNNGC 3 cut(s) 33, 527, 628
BstNI CCWGG 1 cut(s) 103
BstSCI CCNGG 1 cut(s) 101
BstV1I GCAGC 2 cut(s) 80, 313
BstX2I RGATCY 1 cut(s) 38
BstXI CCANNNNNNTGG 1 cut(s) 556
BstYI RGATCY 1 cut(s) 38
Bsu15I ATCGAT 1 cut(s) 459
BsuTUI ATCGAT 1 cut(s) 459
BtgI CCRYGG 1 cut(s) 31
BtsCI GGATG 5 cut(s) 27, 95, 546, 565, 680
Cac8I GCNNGC 1 cut(s) 624
Cfr10I RCCGGY 1 cut(s) 647
Cfr13I GGNCC 1 cut(s) 634
ClaI ATCGAT 1 cut(s) 459
CviJI RGCY 9 cut(s) 71, 106, 263, 326, 406, 521, 530, 601, 651
CviKI_1 RGCY 9 cut(s) 71, 106, 263, 326, 406, 521, 530, 601, 651
DdeI CTNAG 2 cut(s) 183, 407
DpnI GATC 4 cut(s) 40, 300, 478, 658
DpnII GATC 4 cut(s) 38, 298, 476, 656
EciI GGCGGA 3 cut(s) 4, 11, 51
Eco130I CCWWGG 1 cut(s) 593
Eco47I GGWCC 1 cut(s) 634
Eco57I CTGAAG 2 cut(s) 389, 637
Eco88I CYCGRG 1 cut(s) 382
EcoO109I RGGNCCY 1 cut(s) 634
EcoRII CCWGG 1 cut(s) 101
EcoT14I CCWWGG 1 cut(s) 593
ErhI CCWWGG 1 cut(s) 593
FaiI YATR 2 cut(s) 354, 502
FblI GTMKAC 1 cut(s) 579
Fnu4HI GCNGC 3 cut(s) 25, 69, 327
FokI GGATG 4 cut(s) 14, 82, 553, 572
Fsp4HI GCNGC 3 cut(s) 25, 69, 327
FspBI CTAG 3 cut(s) 531, 594, 603
GluI GCNGC 3 cut(s) 25, 69, 327
HapII CCGG 1 cut(s) 648
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HindIII AAGCTT 2 cut(s) 261, 404
HpaII CCGG 1 cut(s) 648
HphI GGTGA 1 cut(s) 209
Hpy166II GTNNAC 2 cut(s) 365, 580
Hpy188I TCNGA 3 cut(s) 643, 656, 674
Hpy188III TCNNGA 2 cut(s) 382, 384
Hpy8I GTNNAC 2 cut(s) 365, 580
HpyAV CCTTC 3 cut(s) 28, 530, 648
HpyCH4III ACNGT 1 cut(s) 377
HpyCH4V TGCA 1 cut(s) 329
HpyF10VI GCNNNNNNNGC 3 cut(s) 33, 527, 628
HpyF3I CTNAG 2 cut(s) 183, 407
Kzo9I GATC 4 cut(s) 38, 298, 476, 656
LmnI GCTCC 1 cut(s) 598
LpnPI CCDG 5 cut(s) 88, 115, 597, 617, 661
Lsp1109I GCAGC 2 cut(s) 80, 313
LweI GCATC 3 cut(s) 36, 433, 515
MaeI CTAG 3 cut(s) 531, 594, 603
MaeIII GTNAC 1 cut(s) 77
MalI GATC 4 cut(s) 40, 300, 478, 658
MbiI CCGCTC 1 cut(s) 50
MboI GATC 4 cut(s) 38, 298, 476, 656
MboII GAAGA 3 cut(s) 33, 382, 527
MflI RGATCY 1 cut(s) 38
MluCI AATT 4 cut(s) 62, 82, 140, 282
MnlI CCTC 7 cut(s) 55, 144, 193, 406, 621, 624, 627
MseI TTAA 2 cut(s) 179, 389
MspA1I CMGCKG 1 cut(s) 117
MspI CCGG 1 cut(s) 648
MspR9I CCNGG 1 cut(s) 103
Mva1269I GAATGC 1 cut(s) 550
MvaI CCWGG 1 cut(s) 103
MwoI GCNNNNNNNGC 3 cut(s) 33, 527, 628
NdeII GATC 4 cut(s) 38, 298, 476, 656
NlaIV GGNNCC 2 cut(s) 600, 636
NmeAIII GCCGAG 1 cut(s) 547
NmuCI GTSAC 1 cut(s) 77
PaeR7I CTCGAG 1 cut(s) 382
PctI GAATGC 1 cut(s) 550
PflMI CCANNNNNTGG 1 cut(s) 675
PkrI GCNGC 3 cut(s) 26, 70, 328
PpuMI RGGWCCY 1 cut(s) 634
Psp5II RGGWCCY 1 cut(s) 634
Psp6I CCWGG 1 cut(s) 101
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 2 cut(s) 600, 636
PspPI GGNCC 1 cut(s) 634
PspPPI RGGWCCY 1 cut(s) 634
PsuI RGATCY 1 cut(s) 38
SalI GTCGAC 1 cut(s) 578
SaqAI TTAA 2 cut(s) 179, 389
SatI GCNGC 3 cut(s) 25, 69, 327
Sau3AI GATC 4 cut(s) 38, 298, 476, 656
Sau96I GGNCC 1 cut(s) 634
ScrFI CCNGG 1 cut(s) 103
SetI ASST 5 cut(s) 136, 265, 408, 532, 595
SfaNI GCATC 3 cut(s) 36, 433, 515
Sfr274I CTCGAG 1 cut(s) 382
SinI GGWCC 1 cut(s) 634
SlaI CTCGAG 1 cut(s) 382
SmlI CTYRAG 1 cut(s) 382
SmoI CTYRAG 1 cut(s) 382
Sse9I AATT 4 cut(s) 62, 82, 140, 282
SsiI CCGC 7 cut(s) 15, 22, 25, 36, 48, 117, 620
SspMI CTAG 3 cut(s) 531, 594, 603
StyD4I CCNGG 1 cut(s) 101
StyI CCWWGG 1 cut(s) 593
TaaI ACNGT 1 cut(s) 377
TaqI TCGA 4 cut(s) 301, 383, 459, 579
TasI AATT 4 cut(s) 62, 82, 140, 282
TauI GCSGC 1 cut(s) 27
Tru1I TTAA 2 cut(s) 179, 389
Tru9I TTAA 2 cut(s) 179, 389
TseFI GTSAC 1 cut(s) 77
TseI GCWGC 2 cut(s) 68, 326
Tsp45I GTSAC 1 cut(s) 77
TspDTI ATGAA 3 cut(s) 100, 484, 557
Van91I CCANNNNNTGG 1 cut(s) 675
VpaK11BI GGWCC 1 cut(s) 634
XapI RAATTY 1 cut(s) 140
XhoI CTCGAG 1 cut(s) 382
XmaJI CCTAGG 1 cut(s) 593
XmiI GTMKAC 1 cut(s) 579
XspI CTAG 3 cut(s) 531, 594, 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.