MD12G1138600.v1.1

Glycine-rich RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008734
Physical Location & Seq
Reverse (-)
21600548 .. 21602099
1552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1138600.v1.1.491

Sequence Viewer

Length: 429 bp
ATGGCGTTCATTTCCACCTTCCAACGCGTCCTCGGCGGATCTTCCTCCGCTCTCCACTCCCAATTCGCCGCCATTCGTCACAATTCAACTCTCACATCCCCCAGGCTATTCATCAGCGGTCTTTCGAGATTAACAACGGACGAAAAGCTTAAAGAGGCATTTTCCCCATTTGGACAGATTGTAGATGCTAAGGTGGTCATGGATAGGGTGTCTCAAAGATCAAAGGGGTTCGGTTTCATCACATACGCATCAGTTGAAGAAGCTGAGAAAGCTAGAGAAGGAATGAATGCCAAGTTCTTGGATGGGTGGGTTATTTTCGTCGACCCTGCCAAGCCTAGGGAGCCTAGATATGCCCCTCCACCGCAAGCATCGCAAGACCCTTCAGAAACCGGCTTCAGATCAAACAAGACGATTGGATGGTGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

143

Amino Acids

15.65

Weight (kDa)

9.46

Isoelectric Point (pI)

48.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 36 - 102 2.8e-20 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 50
AccI GTMKAC 1 cut(s) 321
AccII CGCG 1 cut(s) 27
AciI CCGC 5 cut(s) 36, 48, 69, 117, 362
AclWI GGATC 1 cut(s) 46
AcuI CTGAAG 2 cut(s) 366, 379
AfiI CCNNNNNNNGG 1 cut(s) 336
AflIII ACRYGT 1 cut(s) 25
AgsI TTSAA 2 cut(s) 87, 257
AjnI CCWGG 1 cut(s) 101
AluBI AGCT 3 cut(s) 148, 263, 272
AluI AGCT 3 cut(s) 148, 263, 272
Alw26I GTCTC 1 cut(s) 216
AlwI GGATC 1 cut(s) 46
AspA2I CCTAGG 1 cut(s) 335
AvrII CCTAGG 1 cut(s) 335
BccI CCATC 2 cut(s) 296, 411
BciT130I CCWGG 1 cut(s) 103
BcoDI GTCTC 1 cut(s) 216
BfaI CTAG 3 cut(s) 273, 336, 345
BisI GCNGC 1 cut(s) 69
BlnI CCTAGG 1 cut(s) 335
BlsI GCNGC 1 cut(s) 70
Bme1390I CCNGG 1 cut(s) 103
BmiI GGNNCC 1 cut(s) 342
BmrFI CCNGG 1 cut(s) 103
BmsI GCATC 3 cut(s) 175, 257, 377
Bpu10I CCTNAGC 1 cut(s) 189
BsaJI CCNNGG 3 cut(s) 31, 101, 335
Bsc4I CCNNNNNNNGG 1 cut(s) 336
Bse118I RCCGGY 1 cut(s) 389
BseBI CCWGG 1 cut(s) 103
BseDI CCNNGG 3 cut(s) 31, 101, 335
BseGI GGATG 3 cut(s) 95, 307, 422
BseLI CCNNNNNNNGG 1 cut(s) 336
BseMII CTCAG 1 cut(s) 255
Bsh1236I CGCG 1 cut(s) 27
BsiSI CCGG 1 cut(s) 390
BslI CCNNNNNNNGG 1 cut(s) 336
BsmAI GTCTC 1 cut(s) 216
BsmI GAATGC 1 cut(s) 292
Bsp143I GATC 3 cut(s) 38, 218, 398
BspACI CCGC 5 cut(s) 36, 48, 69, 117, 362
BspCNI CTCAG 1 cut(s) 256
BspFNI CGCG 1 cut(s) 27
BspLI GGNNCC 1 cut(s) 342
BspPI GGATC 1 cut(s) 46
BsrBI CCGCTC 1 cut(s) 50
BsrFI RCCGGY 1 cut(s) 389
BssAI RCCGGY 1 cut(s) 389
BssECI CCNNGG 3 cut(s) 31, 101, 335
BssMI GATC 3 cut(s) 38, 218, 398
BssT1I CCWWGG 1 cut(s) 335
Bst2UI CCWGG 1 cut(s) 103
BstC8I GCNNGC 1 cut(s) 366
BstDEI CTNAG 2 cut(s) 189, 264
BstF5I GGATG 3 cut(s) 95, 307, 422
BstFNI CGCG 1 cut(s) 27
BstKTI GATC 3 cut(s) 41, 221, 401
BstMAI GTCTC 1 cut(s) 216
BstMBI GATC 3 cut(s) 38, 218, 398
BstMWI GCNNNNNNNGC 4 cut(s) 33, 269, 340, 370
BstNI CCWGG 1 cut(s) 103
BstSCI CCNGG 1 cut(s) 101
BstUI CGCG 1 cut(s) 27
BstX2I RGATCY 1 cut(s) 38
BstXI CCANNNNNNTGG 1 cut(s) 298
BstYI RGATCY 1 cut(s) 38
BtgZI GCGATG 1 cut(s) 354
BtsCI GGATG 3 cut(s) 95, 307, 422
Cac8I GCNNGC 1 cut(s) 366
Cfr10I RCCGGY 1 cut(s) 389
CseI GACGC 1 cut(s) 16
CviAII CATG 1 cut(s) 199
CviJI RGCY 7 cut(s) 106, 148, 263, 272, 334, 343, 393
CviKI_1 RGCY 7 cut(s) 106, 148, 263, 272, 334, 343, 393
DdeI CTNAG 2 cut(s) 189, 264
DpnI GATC 3 cut(s) 40, 220, 400
DpnII GATC 3 cut(s) 38, 218, 398
EciI GGCGGA 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 335
Eco57I CTGAAG 2 cut(s) 366, 379
EcoRII CCWGG 1 cut(s) 101
EcoT14I CCWWGG 1 cut(s) 335
ErhI CCWWGG 1 cut(s) 335
FaeI CATG 1 cut(s) 202
FaiI YATR 3 cut(s) 200, 244, 351
FatI CATG 1 cut(s) 198
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 1 cut(s) 69
FokI GGATG 2 cut(s) 82, 314
Fsp4HI GCNGC 1 cut(s) 69
FspBI CTAG 3 cut(s) 273, 336, 345
GluI GCNGC 1 cut(s) 69
HapII CCGG 1 cut(s) 390
HgaI GACGC 1 cut(s) 16
Hin1II CATG 1 cut(s) 202
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HindIII AAGCTT 1 cut(s) 146
HpaII CCGG 1 cut(s) 390
Hpy166II GTNNAC 1 cut(s) 322
Hpy188I TCNGA 2 cut(s) 385, 398
Hpy188III TCNNGA 1 cut(s) 126
Hpy8I GTNNAC 1 cut(s) 322
Hpy99I CGWCG 1 cut(s) 323
HpyAV CCTTC 3 cut(s) 28, 272, 390
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 269, 340, 370
HpyF3I CTNAG 2 cut(s) 189, 264
Hsp92II CATG 1 cut(s) 202
Kzo9I GATC 3 cut(s) 38, 218, 398
LmnI GCTCC 1 cut(s) 340
LpnPI CCDG 4 cut(s) 88, 115, 339, 403
LweI GCATC 3 cut(s) 175, 257, 377
MaeI CTAG 3 cut(s) 273, 336, 345
MaeIII GTNAC 1 cut(s) 77
MalI GATC 3 cut(s) 40, 220, 400
MbiI CCGCTC 1 cut(s) 50
MboI GATC 3 cut(s) 38, 218, 398
MboII GAAGA 2 cut(s) 33, 269
MflI RGATCY 1 cut(s) 38
MluCI AATT 2 cut(s) 62, 82
MluI ACGCGT 1 cut(s) 25
MmeI TCCRAC 1 cut(s) 46
MnlI CCTC 4 cut(s) 41, 55, 148, 366
MseI TTAA 2 cut(s) 131, 150
MspA1I CMGCKG 1 cut(s) 117
MspI CCGG 1 cut(s) 390
MspR9I CCNGG 1 cut(s) 103
Mva1269I GAATGC 1 cut(s) 292
MvaI CCWGG 1 cut(s) 103
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 4 cut(s) 33, 269, 340, 370
NdeII GATC 3 cut(s) 38, 218, 398
NlaIII CATG 1 cut(s) 202
NlaIV GGNNCC 1 cut(s) 342
NmeAIII GCCGAG 1 cut(s) 12
NmuCI GTSAC 1 cut(s) 77
PctI GAATGC 1 cut(s) 292
PkrI GCNGC 1 cut(s) 70
Psp6I CCWGG 1 cut(s) 101
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 1 cut(s) 342
PsuI RGATCY 1 cut(s) 38
SalI GTCGAC 1 cut(s) 320
SaqAI TTAA 2 cut(s) 131, 150
SatI GCNGC 1 cut(s) 69
Sau3AI GATC 3 cut(s) 38, 218, 398
ScrFI CCNGG 1 cut(s) 103
SetI ASST 5 cut(s) 20, 150, 195, 265, 274
SfaNI GCATC 3 cut(s) 175, 257, 377
Sse9I AATT 2 cut(s) 62, 82
SsiI CCGC 5 cut(s) 36, 48, 69, 117, 362
SspMI CTAG 3 cut(s) 273, 336, 345
StyD4I CCNGG 1 cut(s) 101
StyI CCWWGG 1 cut(s) 335
TaqI TCGA 2 cut(s) 125, 321
TasI AATT 2 cut(s) 62, 82
TauI GCSGC 1 cut(s) 71
Tru1I TTAA 2 cut(s) 131, 150
Tru9I TTAA 2 cut(s) 131, 150
TseFI GTSAC 1 cut(s) 77
Tsp45I GTSAC 1 cut(s) 77
TspDTI ATGAA 3 cut(s) 100, 226, 299
TspGWI ACGGA 1 cut(s) 152
XmaJI CCTAGG 1 cut(s) 335
XmiI GTMKAC 1 cut(s) 321
XspI CTAG 3 cut(s) 273, 336, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.