Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005150

Physical Location & Seq

Genomic Coordinates Reverse (-)

24114318 .. 24116667

Gene Structure 2350 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1152000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 774 bp

ATGGACATTGCTCTCTTTTCCCCATCTTCGCTTTTTCATGACGACGATGACTCTTCCAGTGGTGAGGAAAACACAGAGAGCCAGCAAAACTTCGTAGAGAGGAGGCACGAGTTTCCTGGGATGGAGTTGGTTGTCCGAGAATTTTCGTTTCATCAGTTGAATGCCAATTTACTCTGGCCTGGGACATTTGCATTTGCAGAATGGTTAGTTGAACACAGGCCATCAATTGAAGGAAGGCATTGTCTTGAACTGGGCAGTGGCACGGGTGCTTTGGCAATTTTTCTACGAAGATTGTTTAATCTTGACATTACGACATCAGACTATAATGATCAGGAAATTGAAGATAATATAGCTCATAACTGCAGGGTAAATGGAATTACACCTGTCCTTCCTCATATAAAGCATACATGGGGTGACAGTTTCCCTACTGCTGATCCAGACTGGGACCTAATCATTGCTAGTGATATCTTATTGTATGTGAAGCAGTATCCAAATTTGATAAAAACCCTTTTGTTTCTCCTCAAATCTTACAAGCCGAAGGATGGTAAAGCACAGCCTCAAGCAAAGGATGAACAGAGTGGTGGAGACTCTTTTAGTTTTCCCCGGCCAGCATTTCTGATGAGTTGGAGACGCAGAATCGGAAAAGAGGATGAATCACTTTTCTTCACTGGTTGCGAGGATGCTGGCCTTGAAGTAAAGCATCTGGGATCTCGTGTATACTGCATCAAACCAAGAGAAGCAACTTCCCACGGCGACCAAATAGAGAAGGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_16

Protein Analysis

258

Amino Acids

29.24

Weight (kDa)

5.11

Isoelectric Point (pI)

53.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_16	PF10294	55 - 175	5.9e-13	Lysine methyltransferase
Methyltransf_23	PF13489	77 - 175	6.3e-06	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005150)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01470 AT5G01470 AT5G01470 AT5G01470 AT5G01470 AT5G01470 AT5G01470
fragaria_vesca	FvH4_6g11480 FvH4_6g11480 FvH4_6g11480 FvH4_6g11480 FvH4_6g11480 FvH4_6g11480
malus_domestica	MD04G1152000.v1.1
prunus_persica	Prupe.6G277100_v2.0.a1
pyrus_communis	pycom04g13800
rosa_chinensis	RchiOBHm_Chr3g0463171
rosa_laevigata	RLG00000024806
rosa_multiflora	Rmu_sc0001916.1_g000012 Rmu_sc0008079.1_g000006 Rmu_sc0008124.1_g000004
rosa_roxburghii	Rroxscaffold_6G00416860 Rroxscaffold_6G00416910
rosa_rugosa	Rorug03G0061900 Rorug03G0062000
rosa_samantha	Rh3BG124600 Rh3CG126600 Rh3DG125600
rosa_wichuraiana	Rw3G010170 Rw3G010200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	717
AclWI	GGATC	2 cut(s)	428, 715
AcoI	YGGCCR	1 cut(s)	605
AcsI	RAATTY	2 cut(s)	140, 493
AfiI	CCNNNNNNNGG	1 cut(s)	542
AgsI	TTSAA	6 cut(s)	160, 212, 230, 248, 341, 692
AjnI	CCWGG	2 cut(s)	115, 178
AluBI	AGCT	1 cut(s)	353
AluI	AGCT	1 cut(s)	353
Alw26I	GTCTC	2 cut(s)	579, 622
AlwI	GGATC	2 cut(s)	428, 715
AoxI	GGCC	4 cut(s)	176, 218, 605, 685
ApoI	RAATTY	2 cut(s)	140, 493
AspS9I	GGNCC	1 cut(s)	445
AsuC2I	CCSGG	1 cut(s)	604
AsuHPI	GGTGA	2 cut(s)	74, 425
AvaII	GGWCC	1 cut(s)	445
BauI	CACGAG	2 cut(s)	107, 711
BccI	CCATC	4 cut(s)	31, 115, 229, 536
BceAI	ACGGC	1 cut(s)	766
BciT130I	CCWGG	2 cut(s)	117, 180
BciVI	GTATCC	1 cut(s)	498
BclI	TGATCA	1 cut(s)	328
BcnI	CCSGG	1 cut(s)	604
BcoDI	GTCTC	2 cut(s)	579, 622
BfaI	CTAG	1 cut(s)	459
BfmI	CTRYAG	1 cut(s)	361
BfuI	GTATCC	1 cut(s)	498
Bme1390I	CCNGG	3 cut(s)	117, 180, 604
Bme18I	GGWCC	1 cut(s)	445
BmgT120I	GGNCC	1 cut(s)	445
BmiI	GGNNCC	1 cut(s)	446
BmrFI	CCNGG	3 cut(s)	117, 180, 604
BmrI	ACTGGG	2 cut(s)	260, 451
BmsI	GCATC	3 cut(s)	670, 709, 732
BmuI	ACTGGG	2 cut(s)	260, 451
BpuEI	CTTGAG	1 cut(s)	543
BpuMI	CCSGG	1 cut(s)	604
BsaJI	CCNNGG	4 cut(s)	116, 179, 602, 748
Bsc4I	CCNNNNNNNGG	1 cut(s)	542
Bse1I	ACTGG	4 cut(s)	57, 255, 446, 673
Bse3DI	GCAATG	2 cut(s)	6, 453
BseBI	CCWGG	2 cut(s)	117, 180
BseDI	CCNNGG	4 cut(s)	116, 179, 602, 748
BseGI	GGATG	5 cut(s)	126, 547, 574, 655, 685
BseLI	CCNNNNNNNGG	1 cut(s)	542
BseMI	GCAATG	2 cut(s)	6, 453
BseNI	ACTGG	4 cut(s)	57, 255, 446, 673
BseRI	GAGGAG	2 cut(s)	115, 509
BshFI	GGCC	4 cut(s)	178, 220, 607, 687
BsiSI	CCGG	1 cut(s)	604
BslFI	GGGAC	2 cut(s)	196, 458
BslI	CCNNNNNNNGG	1 cut(s)	542
BsmAI	GTCTC	2 cut(s)	579, 622
BsmBI	CGTCTC	1 cut(s)	622
BsmFI	GGGAC	2 cut(s)	196, 458
BsmI	GAATGC	1 cut(s)	166
BsnI	GGCC	4 cut(s)	178, 220, 607, 687
Bsp143I	GATC	3 cut(s)	328, 433, 707
BspANI	GGCC	4 cut(s)	178, 220, 607, 687
BspHI	TCATGA	1 cut(s)	37
BspLI	GGNNCC	1 cut(s)	446
BspMAI	CTGCAG	1 cut(s)	365
BspPI	GGATC	2 cut(s)	428, 715
BsrDI	GCAATG	2 cut(s)	6, 453
BsrI	ACTGG	4 cut(s)	57, 255, 446, 673
BssECI	CCNNGG	4 cut(s)	116, 179, 602, 748
BssMI	GATC	3 cut(s)	328, 433, 707
BssNAI	GTATAC	1 cut(s)	718
BssSI	CACGAG	2 cut(s)	107, 711
Bst1107I	GTATAC	1 cut(s)	718
Bst2BI	CACGAG	2 cut(s)	107, 711
Bst2UI	CCWGG	2 cut(s)	117, 180
Bst4CI	ACNGT	1 cut(s)	419
Bst6I	CTCTTC	1 cut(s)	58
BstC8I	GCNNGC	3 cut(s)	83, 609, 685
BstDSI	CCRYGG	1 cut(s)	748
BstF5I	GGATG	5 cut(s)	126, 547, 574, 655, 685
BstKTI	GATC	3 cut(s)	331, 436, 710
BstMAI	GTCTC	2 cut(s)	579, 622
BstMBI	GATC	3 cut(s)	328, 433, 707
BstNI	CCWGG	2 cut(s)	117, 180
BstSCI	CCNGG	3 cut(s)	115, 178, 602
BstSFI	CTRYAG	1 cut(s)	361
BstX2I	RGATCY	1 cut(s)	707
BstYI	RGATCY	1 cut(s)	707
BstZ17I	GTATAC	1 cut(s)	718
BsuI	GTATCC	1 cut(s)	498
BsuRI	GGCC	4 cut(s)	178, 220, 607, 687
BtgI	CCRYGG	1 cut(s)	748
BtsCI	GGATG	5 cut(s)	126, 547, 574, 655, 685
BtsI	GCAGTG	1 cut(s)	262
BtsIMutI	CAGTG	3 cut(s)	64, 262, 666
Cac8I	GCNNGC	3 cut(s)	83, 609, 685
CciI	TCATGA	1 cut(s)	37
Cfr13I	GGNCC	1 cut(s)	445
CseI	GACGC	1 cut(s)	639
CviAII	CATG	2 cut(s)	38, 408
CviJI	RGCY	8 cut(s)	81, 178, 220, 353, 535, 556, 607, 687
CviKI_1	RGCY	8 cut(s)	81, 178, 220, 353, 535, 556, 607, 687
DpnI	GATC	3 cut(s)	330, 435, 709
DpnII	GATC	3 cut(s)	328, 433, 707
EaeI	YGGCCR	1 cut(s)	605
Eam1104I	CTCTTC	1 cut(s)	58
EarI	CTCTTC	1 cut(s)	58
Eco32I	GATATC	1 cut(s)	466
Eco47I	GGWCC	1 cut(s)	445
EcoO109I	RGGNCCY	1 cut(s)	445
EcoRII	CCWGG	2 cut(s)	115, 178
EcoRV	GATATC	1 cut(s)	466
Esp3I	CGTCTC	1 cut(s)	622
FaeI	CATG	2 cut(s)	41, 411
FaqI	GGGAC	2 cut(s)	196, 458
FatI	CATG	2 cut(s)	37, 407
FbaI	TGATCA	1 cut(s)	328
FblI	GTMKAC	1 cut(s)	717
FokI	GGATG	5 cut(s)	133, 554, 581, 662, 692
FspBI	CTAG	1 cut(s)	459
HaeIII	GGCC	4 cut(s)	178, 220, 607, 687
HapII	CCGG	1 cut(s)	604
HgaI	GACGC	1 cut(s)	639
Hin1II	CATG	2 cut(s)	41, 411
HinfI	GANTC	4 cut(s)	50, 587, 636, 653
HpaII	CCGG	1 cut(s)	604
HphI	GGTGA	2 cut(s)	74, 425
Hpy166II	GTNNAC	1 cut(s)	718
Hpy188I	TCNGA	4 cut(s)	137, 319, 618, 641
Hpy188III	TCNNGA	5 cut(s)	38, 245, 302, 332, 437
Hpy8I	GTNNAC	1 cut(s)	718
Hpy99I	CGWCG	1 cut(s)	47
HpyAV	CCTTC	5 cut(s)	224, 228, 398, 532, 760
HpyCH4III	ACNGT	1 cut(s)	419
HpyCH4V	TGCA	4 cut(s)	191, 197, 363, 723
Hsp92II	CATG	2 cut(s)	41, 411
Ksp22I	TGATCA	1 cut(s)	328
Kzo9I	GATC	3 cut(s)	328, 433, 707
LweI	GCATC	3 cut(s)	670, 709, 732
MaeI	CTAG	1 cut(s)	459
MaeIII	GTNAC	1 cut(s)	413
MalI	GATC	3 cut(s)	330, 435, 709
MboI	GATC	3 cut(s)	328, 433, 707
MboII	GAAGA	5 cut(s)	18, 45, 300, 353, 655
MfeI	CAATTG	1 cut(s)	225
MflI	RGATCY	1 cut(s)	707
MluCI	AATT	7 cut(s)	140, 166, 225, 276, 336, 375, 493
MlyI	GAGTC	2 cut(s)	44, 581
MmeI	TCCRAC	1 cut(s)	605
MnlI	CCTC	8 cut(s)	58, 93, 96, 402, 530, 567, 640, 670
MseI	TTAA	1 cut(s)	297
MspI	CCGG	1 cut(s)	604
MspR9I	CCNGG	3 cut(s)	117, 180, 604
MunI	CAATTG	1 cut(s)	225
Mva1269I	GAATGC	1 cut(s)	166
MvaI	CCWGG	2 cut(s)	117, 180
NciI	CCSGG	1 cut(s)	604
NdeII	GATC	3 cut(s)	328, 433, 707
NlaIII	CATG	2 cut(s)	41, 411
NlaIV	GGNNCC	1 cut(s)	446
NmuCI	GTSAC	1 cut(s)	413
PagI	TCATGA	1 cut(s)	37
PctI	GAATGC	1 cut(s)	166
PfeI	GAWTC	2 cut(s)	636, 653
PleI	GAGTC	2 cut(s)	44, 581
PpsI	GAGTC	2 cut(s)	44, 581
PpuMI	RGGWCCY	1 cut(s)	445
Psp5II	RGGWCCY	1 cut(s)	445
Psp6I	CCWGG	2 cut(s)	115, 178
PspGI	CCWGG	2 cut(s)	115, 178
PspN4I	GGNNCC	1 cut(s)	446
PspPI	GGNCC	1 cut(s)	445
PspPPI	RGGWCCY	1 cut(s)	445
PstI	CTGCAG	1 cut(s)	365
PsuI	RGATCY	1 cut(s)	707
SaqAI	TTAA	1 cut(s)	297
Sau3AI	GATC	3 cut(s)	328, 433, 707
Sau96I	GGNCC	1 cut(s)	445
SchI	GAGTC	2 cut(s)	44, 581
ScrFI	CCNGG	3 cut(s)	117, 180, 604
SetI	ASST	4 cut(s)	355, 385, 450, 771
SfaNI	GCATC	3 cut(s)	670, 709, 732
SfcI	CTRYAG	1 cut(s)	361
SinI	GGWCC	1 cut(s)	445
SmlI	CTYRAG	1 cut(s)	558
SmoI	CTYRAG	1 cut(s)	558
Sse9I	AATT	7 cut(s)	140, 166, 225, 276, 336, 375, 493
SspMI	CTAG	1 cut(s)	459
StyD4I	CCNGG	3 cut(s)	115, 178, 602
TaaI	ACNGT	1 cut(s)	419
TasI	AATT	7 cut(s)	140, 166, 225, 276, 336, 375, 493
TfiI	GAWTC	2 cut(s)	636, 653
Tru1I	TTAA	1 cut(s)	297
Tru9I	TTAA	1 cut(s)	297
TscAI	CASTG	3 cut(s)	64, 262, 673
TseFI	GTSAC	1 cut(s)	413
Tsp45I	GTSAC	1 cut(s)	413
TspDTI	ATGAA	4 cut(s)	26, 140, 585, 666
TspRI	CASTG	3 cut(s)	64, 262, 673
VpaK11BI	GGWCC	1 cut(s)	445
XapI	RAATTY	2 cut(s)	140, 493
XmiI	GTMKAC	1 cut(s)	717
XspI	CTAG	1 cut(s)	459

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.