Rw3G010200

Protein-lysine methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005150
Physical Location & Seq
Forward (+)
9805352 .. 9807956
2605 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G010200.1

Sequence Viewer

Length: 777 bp
ATGGACATAGCTCTCTTTTCCCCATCTTCCCTTTTTGCCAACGACGATGACTCTTCCAGTGGTTTCCTGGAAAACACAGAGACCCAGCAAAGCTTCGTAGAGAGGAAGCATGAGTTTCCTGGAATGGAATTGATCGTTCGAGAATTTTCATTTCATCAGCTGAATGCCAATTTACTCTGGCCGGGGACATTTGCATTTGCAGAATGGTTAGTTCAAAACAGGCCATTGATTGAGGGACGGCATTGTATTGAACTGGGCAGTGGTACTGGTGCTTTGGCCATTTTCCTACTAAGATTGTGTAATCTTGACATCACTACATCAGACTATAATGATCAAGAAATTGAAGAGAATATAGCTTACAACTGCAGGGTAAATGGAATTGCACCTGCTCTCCCTCATATAAAGCATACGTGGGGTGACAGCTTCCCCAATAGGGATCCAGACTGGGACCTAATTATTGCGAGTGATATCTTATTGTATGTGAAACAGTATCCAAATTTGATAAAAACTCTTTCGTTTCTCCTCACATCTTACAAGCCAAAGGAGAATGAATCACAGCCTCGACCCAAGGACGAGCCAACTGGTAGACAATCTTTCAGTTTACCTCGGCCGTCATTTGTGATGAGTTGGAGACGAAGAATTGGAAAAGAGGACGAATCACTGTTCTTCACAGGGTGTGAGGATTCTTGTCTTGAAGTAAAGCATCTGGGATCCCGGGTGTATTGCATCAAACCTAGAGAAACTATTACAAACGGCGACCAAATAGTGAATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

258

Amino Acids

29.47

Weight (kDa)

5.04

Isoelectric Point (pI)

49.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 55 - 176 7.3e-13 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 394
Acc36I ACCTGC 1 cut(s) 394
AccI GTMKAC 1 cut(s) 586
AclWI GGATC 4 cut(s) 431, 444, 705, 718
AcoI YGGCCR 3 cut(s) 179, 276, 608
AcsI RAATTY 3 cut(s) 143, 496, 769
AdeI CACNNNGTG 1 cut(s) 675
AfaI GTAC 1 cut(s) 265
AfiI CCNNNNNNNGG 1 cut(s) 433
AgsI TTSAA 4 cut(s) 215, 251, 344, 695
AjnI CCWGG 2 cut(s) 66, 118
AluBI AGCT 5 cut(s) 11, 93, 160, 356, 423
AluI AGCT 5 cut(s) 11, 93, 160, 356, 423
Alw26I GTCTC 2 cut(s) 74, 625
AlwI GGATC 4 cut(s) 431, 444, 705, 718
Ama87I CYCGRG 1 cut(s) 714
AoxI GGCC 4 cut(s) 179, 221, 276, 608
ApoI RAATTY 3 cut(s) 143, 496, 769
AspS9I GGNCC 1 cut(s) 448
AsuC2I CCSGG 3 cut(s) 183, 715, 716
AsuHPI GGTGA 1 cut(s) 428
AvaI CYCGRG 1 cut(s) 714
AvaII GGWCC 1 cut(s) 448
BalI TGGCCA 1 cut(s) 278
BamHI GGATCC 2 cut(s) 436, 710
BccI CCATC 1 cut(s) 31
BceAI ACGGC 3 cut(s) 254, 595, 769
BciT130I CCWGG 2 cut(s) 68, 120
BciVI GTATCC 1 cut(s) 501
BclI TGATCA 1 cut(s) 331
BcnI CCSGG 3 cut(s) 183, 715, 716
BcoDI GTCTC 2 cut(s) 74, 625
BfaI CTAG 1 cut(s) 735
BfmI CTRYAG 1 cut(s) 364
BfuAI ACCTGC 1 cut(s) 394
BfuI GTATCC 1 cut(s) 501
Bme1390I CCNGG 5 cut(s) 68, 120, 183, 715, 716
Bme18I GGWCC 1 cut(s) 448
BmeT110I CYCGRG 1 cut(s) 714
BmgT120I GGNCC 1 cut(s) 448
BmiI GGNNCC 3 cut(s) 438, 449, 712
BmrFI CCNGG 5 cut(s) 68, 120, 183, 715, 716
BmrI ACTGGG 2 cut(s) 263, 454
BmsI GCATC 2 cut(s) 712, 735
BmuI ACTGGG 2 cut(s) 263, 454
BpuMI CCSGG 3 cut(s) 183, 715, 716
BsaAI YACGTR 1 cut(s) 411
BsaI GGTCTC 1 cut(s) 74
BsaJI CCNNGG 4 cut(s) 182, 567, 605, 714
BsaXI ACNNNNNCTCC 2 cut(s) 375, 405
Bsc4I CCNNNNNNNGG 1 cut(s) 433
Bse1I ACTGG 5 cut(s) 57, 258, 271, 449, 586
BseBI CCWGG 2 cut(s) 68, 120
BseDI CCNNGG 4 cut(s) 182, 567, 605, 714
BseLI CCNNNNNNNGG 1 cut(s) 433
BseNI ACTGG 5 cut(s) 57, 258, 271, 449, 586
BseRI GAGGAG 1 cut(s) 512
BseX3I CGGCCG 1 cut(s) 608
BseYI CCCAGC 1 cut(s) 84
Bsh1285I CGRYCG 1 cut(s) 611
BshFI GGCC 4 cut(s) 181, 223, 278, 610
BsiEI CGRYCG 1 cut(s) 611
BsiHKCI CYCGRG 1 cut(s) 714
BsiSI CCGG 2 cut(s) 182, 715
BslFI GGGAC 3 cut(s) 199, 249, 461
BslI CCNNNNNNNGG 1 cut(s) 433
BsmAI GTCTC 2 cut(s) 74, 625
BsmBI CGTCTC 1 cut(s) 625
BsmFI GGGAC 3 cut(s) 199, 249, 461
BsmI GAATGC 1 cut(s) 169
BsnI GGCC 4 cut(s) 181, 223, 278, 610
Bso31I GGTCTC 1 cut(s) 74
BsoBI CYCGRG 1 cut(s) 714
Bsp143I GATC 4 cut(s) 132, 331, 436, 710
BspANI GGCC 4 cut(s) 181, 223, 278, 610
BspLI GGNNCC 3 cut(s) 438, 449, 712
BspMAI CTGCAG 1 cut(s) 368
BspMI ACCTGC 1 cut(s) 394
BspPI GGATC 4 cut(s) 431, 444, 705, 718
BspTNI GGTCTC 1 cut(s) 74
BsrI ACTGG 5 cut(s) 57, 258, 271, 449, 586
BssECI CCNNGG 4 cut(s) 182, 567, 605, 714
BssMI GATC 4 cut(s) 132, 331, 436, 710
BssT1I CCWWGG 1 cut(s) 567
Bst2UI CCWGG 2 cut(s) 68, 120
Bst4CI ACNGT 2 cut(s) 489, 663
Bst6I CTCTTC 2 cut(s) 58, 339
BstBAI YACGTR 1 cut(s) 411
BstDEI CTNAG 2 cut(s) 290, 774
BstKTI GATC 4 cut(s) 135, 334, 439, 713
BstMAI GTCTC 2 cut(s) 74, 625
BstMBI GATC 4 cut(s) 132, 331, 436, 710
BstMCI CGRYCG 1 cut(s) 611
BstNI CCWGG 2 cut(s) 68, 120
BstSCI CCNGG 5 cut(s) 66, 118, 181, 713, 714
BstSFI CTRYAG 1 cut(s) 364
BstX2I RGATCY 2 cut(s) 436, 710
BstYI RGATCY 2 cut(s) 436, 710
BstZI CGGCCG 1 cut(s) 608
BsuI GTATCC 1 cut(s) 501
BsuRI GGCC 4 cut(s) 181, 223, 278, 610
BtsI GCAGTG 1 cut(s) 265
BtsIMutI CAGTG 3 cut(s) 64, 265, 659
BveI ACCTGC 1 cut(s) 394
Cfr13I GGNCC 1 cut(s) 448
Cfr9I CCCGGG 1 cut(s) 714
Csp6I GTAC 1 cut(s) 264
CviAII CATG 1 cut(s) 110
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 2 cut(s) 290, 774
DpnI GATC 4 cut(s) 134, 333, 438, 712
DpnII GATC 4 cut(s) 132, 331, 436, 710
DraIII CACNNNGTG 1 cut(s) 675
EaeI YGGCCR 3 cut(s) 179, 276, 608
EagI CGGCCG 1 cut(s) 608
Eam1104I CTCTTC 2 cut(s) 58, 339
EarI CTCTTC 2 cut(s) 58, 339
EclXI CGGCCG 1 cut(s) 608
Eco130I CCWWGG 1 cut(s) 567
Eco31I GGTCTC 1 cut(s) 74
Eco32I GATATC 1 cut(s) 469
Eco47I GGWCC 1 cut(s) 448
Eco52I CGGCCG 1 cut(s) 608
Eco88I CYCGRG 1 cut(s) 714
EcoO109I RGGNCCY 1 cut(s) 448
EcoRI GAATTC 1 cut(s) 769
EcoRII CCWGG 2 cut(s) 66, 118
EcoRV GATATC 1 cut(s) 469
EcoT14I CCWWGG 1 cut(s) 567
ErhI CCWWGG 1 cut(s) 567
Esp3I CGTCTC 1 cut(s) 625
FaeI CATG 1 cut(s) 113
FaiI YATR 8 cut(s) 8, 111, 327, 353, 399, 401, 408, 480
FaqI GGGAC 3 cut(s) 199, 249, 461
FatI CATG 1 cut(s) 109
FbaI TGATCA 1 cut(s) 331
FblI GTMKAC 1 cut(s) 586
FspBI CTAG 1 cut(s) 735
GsaI CCCAGC 1 cut(s) 88
HaeIII GGCC 4 cut(s) 181, 223, 278, 610
HapII CCGG 2 cut(s) 182, 715
Hin1II CATG 1 cut(s) 113
HindIII AAGCTT 1 cut(s) 91
HinfI GANTC 4 cut(s) 50, 551, 656, 683
HpaII CCGG 2 cut(s) 182, 715
HphI GGTGA 1 cut(s) 428
Hpy166II GTNNAC 2 cut(s) 587, 602
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 5 cut(s) 140, 305, 335, 440, 692
Hpy8I GTNNAC 2 cut(s) 587, 602
Hpy99I CGWCG 1 cut(s) 47
HpyCH4III ACNGT 2 cut(s) 489, 663
HpyCH4IV ACGT 1 cut(s) 410
HpyCH4V TGCA 5 cut(s) 194, 200, 366, 383, 726
HpyF3I CTNAG 2 cut(s) 290, 774
HpySE526I ACGT 1 cut(s) 410
Hsp92II CATG 1 cut(s) 113
Ksp22I TGATCA 1 cut(s) 331
Kzo9I GATC 4 cut(s) 132, 331, 436, 710
LweI GCATC 2 cut(s) 712, 735
MaeI CTAG 1 cut(s) 735
MaeII ACGT 1 cut(s) 410
MaeIII GTNAC 1 cut(s) 416
MalI GATC 4 cut(s) 134, 333, 438, 712
MboI GATC 4 cut(s) 132, 331, 436, 710
MboII GAAGA 5 cut(s) 18, 45, 356, 648, 658
MflI RGATCY 2 cut(s) 436, 710
MlsI TGGCCA 1 cut(s) 278
MluCI AATT 9 cut(s) 128, 143, 169, 339, 378, 453, 496, 639, 769
MluNI TGGCCA 1 cut(s) 278
MlyI GAGTC 1 cut(s) 44
MmeI TCCRAC 1 cut(s) 608
MnlI CCTC 8 cut(s) 96, 226, 405, 533, 570, 615, 643, 673
Mox20I TGGCCA 1 cut(s) 278
MscI TGGCCA 1 cut(s) 278
Msp20I TGGCCA 1 cut(s) 278
MspA1I CMGCKG 1 cut(s) 160
MspI CCGG 2 cut(s) 182, 715
MspR9I CCNGG 5 cut(s) 68, 120, 183, 715, 716
Mva1269I GAATGC 1 cut(s) 169
MvaI CCWGG 2 cut(s) 68, 120
NciI CCSGG 3 cut(s) 183, 715, 716
NdeII GATC 4 cut(s) 132, 331, 436, 710
NlaIII CATG 1 cut(s) 113
NlaIV GGNNCC 3 cut(s) 438, 449, 712
NmeAIII GCCGAG 1 cut(s) 586
NmuCI GTSAC 1 cut(s) 416
PaqCI CACCTGC 1 cut(s) 394
PctI GAATGC 1 cut(s) 169
PfeI GAWTC 3 cut(s) 551, 656, 683
PfoI TCCNGGA 2 cut(s) 66, 118
PleI GAGTC 1 cut(s) 44
PpsI GAGTC 1 cut(s) 44
Ppu21I YACGTR 1 cut(s) 411
PpuMI RGGWCCY 1 cut(s) 448
Psp5II RGGWCCY 1 cut(s) 448
Psp6I CCWGG 2 cut(s) 66, 118
PspFI CCCAGC 1 cut(s) 84
PspGI CCWGG 2 cut(s) 66, 118
PspN4I GGNNCC 3 cut(s) 438, 449, 712
PspPI GGNCC 1 cut(s) 448
PspPPI RGGWCCY 1 cut(s) 448
PstI CTGCAG 1 cut(s) 368
PsuI RGATCY 2 cut(s) 436, 710
PvuII CAGCTG 1 cut(s) 160
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
Sau3AI GATC 4 cut(s) 132, 331, 436, 710
Sau96I GGNCC 1 cut(s) 448
SchI GAGTC 1 cut(s) 44
ScrFI CCNGG 5 cut(s) 68, 120, 183, 715, 716
SfaNI GCATC 2 cut(s) 712, 735
SfcI CTRYAG 1 cut(s) 364
SinI GGWCC 1 cut(s) 448
SmaI CCCGGG 1 cut(s) 716
Sse9I AATT 9 cut(s) 128, 143, 169, 339, 378, 453, 496, 639, 769
SspMI CTAG 1 cut(s) 735
StyD4I CCNGG 5 cut(s) 66, 118, 181, 713, 714
StyI CCWWGG 1 cut(s) 567
TaaI ACNGT 2 cut(s) 489, 663
TaiI ACGT 1 cut(s) 413
TaqI TCGA 2 cut(s) 139, 562
TasI AATT 9 cut(s) 128, 143, 169, 339, 378, 453, 496, 639, 769
TfiI GAWTC 3 cut(s) 551, 656, 683
TscAI CASTG 3 cut(s) 64, 265, 666
TseFI GTSAC 1 cut(s) 416
Tsp45I GTSAC 1 cut(s) 416
TspDTI ATGAA 3 cut(s) 138, 143, 564
TspMI CCCGGG 1 cut(s) 714
TspRI CASTG 3 cut(s) 64, 265, 666
VpaK11BI GGWCC 1 cut(s) 448
XapI RAATTY 3 cut(s) 143, 496, 769
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
XmaI CCCGGG 1 cut(s) 714
XmiI GTMKAC 1 cut(s) 586
XspI CTAG 1 cut(s) 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.