Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0004412

Physical Location & Seq

Genomic Coordinates Forward (+)

25701425 .. 25706105

Gene Structure 4681 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1166800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2856 bp

ATGGCGGACCACGTGCTCCTCAGGTGCGGTCACCCTCTCATCCTCCCGACAATTTCCGCCGACGAACCTCTCTCTCCCGAAGTCCTGCCTCCCCTGAGATCCCTCATCGTCAACCCCTCAACTCCCGGGCCCACAATCACCTCCATCCTCGAAACCCTAACTCGCTCTCTCCCACTCAGTCGCGATCCGCTCACCATCCGGTTCACTCTCAAGCTCCTCTCCGACCTCGCCTCGCGACACCCCCACCTCTCGTCCTTCGTATTCGACTCGGTCCGTTCCCACCTCCTCTCCACCGGCTCGCCCCGCGTTGCTGCCGACTCACTCGACGCGCTTGCTTCAATCGTGGAGAGCAACCGAGCCCTAGCTCCCGGAATTGAAGAATTGGATGACCGGCTGTTCGCCTCGCTGTGTTTCAGTCCGTCTTGCTCCGTACGGCCGTGGCTGCTCAGAAACGCCGAGAGGTTCGGCGTACAGCCTCATTTGCTGTTCACTCTGTTTCTAGGGTTTACGAAGGATCCATATCCGAATGTGAGAAAAGAGGCGTTGGACGGGCTCGTTGGGTTGAGTGAGAACGGTGTGATCGAGGACCGTGACATGATCGAAGGTTGCTATTTTCGTGCCGTTGAGCTCCTAAACGACATGGAAGATTGCGTTCGATTGGCTGCCGTTCGCACGGTGTGTTCTTGGGGTCTAATGCTCGTGGCGTGCATTTCGGAGATGAAAGCATATTGGTCTGCTGAAGTATTTGTCAAACTTTGTTCAATGGTGAGAGACATGAGCATGGAGGTTAGGGTTGAAGCTTTTTATGCTCTTGGGAAGATAAAGTTGGTATCTGAGGATATTCTTTTGCAAACCCTGTCAAAGAGAGTTCTGGTAACCACGAAAGGAAAAGGATCTTTTGCTCAGTGCTCTGATGAACAACTAGAAGTATCAGGTTCAAGTGTTGCTGGAGCTTTCGTGCACGGACTAGAGGATGAATTTCACGAGGTGCGAAAAGGTGCCTGTCATGCATTGAGAACACTGGCCATACTCTCTGCCAAGTTTGCTGGAGAAGCCTTAAACTTATTGATGGATGTACTGAATGATGACTCAATACTTGTTCGGTTACAAGCTTTTGAAACTATGCATCAATTGGCTACCTTTGACCTTCTGAAGGTGCAAGAAGCACACATGCATATGTTTCTTGGCACTCTGGTTGACAATGATACCCTCATAAGATCTTCCGCAAGGAAAGTACTTACATTGGCAAAGCTGCCTCAGTTAAAGATGTTTAGGTTAACTATTGATGCCCTTCTAGAAAATATGGAAAGATATCCACAGGATGAAGCAGATGCATTATCTGTTTTGTTTCATATTGGACGAAATCATGGAAAGTTTGTGGTCCGCATGATTGAGGAAGTTTCTCCACAGATGGAGCCAATGTCTAATGGAAAGTTGGACTTTGATAGCGTGAGAGTGGCCGGCTTGCTAGTTCTGGCCATCTCAGCTCCAGTTTCAGATGAACGTGATTGCAATATTCCACCTGCAATATTTTCTTATGCAGTAACGTATCTGGGAAGGATTTCTCATGCACTGAGTGATATTATCAATCAAAATAGCCTTTTGGATTATTTGTCTCAGTGCAGTAGATCCCAAGGACCATATGATGTAGAGTTCAACAAGTTCAACTTTAAAGCGGGGGAGCCATGCTTACCTTTACTTGAAAATGATGGTTCAACCTGTACTAGTAACAAGATGACTGGTTCAGCTGAAATGTCTGAAATTGTGTCTCCAATAATGGAGCCAAGGGAAGTAGGAACTTCACTTGTAGCATACCAGCTAGAGGTGCATGATGAAGTGACTAAGTTAGTGAATGTTGTTCTTGCAAGGGCCAAGGATATCTGGCCACTAGTACAATCAGGATTCGTGAATGAAGTAATGAGGACTTTGAGAAGTTGCAAGGAAGAGCTGGCAACATTCACTTCTGACTCACTTCCATCTGCGGGTGTTTTGCCCTTCACGAAGCAATATGTTCAGATAATGAAACTGCTTACAAGGGCATGGATGAACTTTCTGCCTTCAGTGCTTTTTCCTCCTTATGGAATGGGAGAATTAGACTTGGTATTAAGAAAGCTGGACACAAGGCTTAGAGATCTGAAGAGTACATTTATAAGATTGTCTAAAAGGGAGGAGTTGCATATCTTGGAGCTGATACTTGTGACCTGTGTGCTCAGATTGTCGAAAGTAGAAATTTGCTGCCATCTTCGAACCTTGAGAAAGCTCTCTAGTACGATGTCACAGGTTGAATCTCTCCTTAGAGATGGATCTCTTGAACCTTCCAGGTTTATAACTGAAGTTGAGAAGTTATCATCGGAAACCGGTACCTTTCCCAATGAAGGTTCCTGTAATCCCCGCCTTTTTCAAAGAGCACTTGAAAGCTTCTCCCTCAAGCAGCTTGTGTTGAGTGGTGGAAACAAGCACGTAAACGCAGAGCTGGATATACCCGACAACAGCTACGAAAATCCTCTCCGGTTTGTCGCAGGACTGCCCGTTGGCATACCCTGCCATATCACACTCCATAACGTTTTAGCCGAAAGTCGGCTGTGGCTGAAGATGACTGTAAATGAAGACGACGATTCAACTCGATTTGTGTTTTTGGATTTAAACCTTTTCGGAGGCTCCGAAGATATCCGGATCTTTACGTTTAGTCCACCCTTTTACAGAACCCCAAAAGCATTTTCTTTCACAATTAAGGTGTGTATCTATATGGAATGTTTGTCCGAGGTTGAGGATGTCTCCTCTGTCAAAAGTCGGGGCCCTAGACATGAAGTAACGTATCTTTGTCGAGAAAAGGATGTTTATCTTTCTATGAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006810 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009956 GO:0009987 GO:0010467 GO:0010496 GO:0012505 GO:0016070 GO:0016073 GO:0016180 GO:0022622 GO:0031123 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0034470 GO:0034472 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043628 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048364 GO:0048731 GO:0048856 GO:0051179 GO:0051234 GO:0071704 GO:0090057 GO:0090304 GO:0097708 GO:0099402 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

952

Amino Acids

106.32

Weight (kDa)

5.66

Isoelectric Point (pI)

48.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
INTS4_C	PF25458	827 - 949	3.8e-12	Integrator subunit 4 family C-terminal Ig-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004412)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08800 AT3G08800 AT3G08800
fragaria_vesca	FvH4_6g10600 FvH4_6g10600 FvH4_6g10600 FvH4_6g10600 FvH4_6g10600
malus_domestica	MD04G1166800.v1.1
prunus_persica	Prupe.6G285000_v2.0.a1 Prupe.6G285000_v2.0.a1
pyrus_communis	pycom01g05880 pycom04g14820
rosa_chinensis	RchiOBHm_Chr3g0461591 RchiOBHm_Chr3g0461601
rosa_laevigata	RLG00000024915
rosa_multiflora	Rmu_co8098260.1_g000001 Rmu_sc0003578.1_g000001 Rmu_sc0032177.1_g000001 Rmu_sc0040016.1_g000001 Rmu_ssc0000193.1_g000007
rosa_roxburghii	Rroxscaffold_6G00418110 Rroxscaffold_6G00418480
rosa_rugosa	Rorug03G0052200 Rorug03G0052200 Rorug03G0052300 Rorug03G0052300
rosa_samantha	Rh3AG110400 Rh3BG113600 Rh3CG115500 Rh3DG115300
rosa_wichuraiana	Rw3G009340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2150, 2327
AarI	CACCTGC	1 cut(s)	1531
Acc36I	ACCTGC	1 cut(s)	1531
Acc65I	GGTACC	1 cut(s)	2360
AccB1I	GGYRCC	2 cut(s)	998, 2360
AccBSI	CCGCTC	1 cut(s)	190
AccII	CGCG	4 cut(s)	183, 235, 306, 329
AccIII	TCCGGA	1 cut(s)	2670
AclI	AACGTT	1 cut(s)	2562
AclWI	GGATC	8 cut(s)	93, 179, 509, 522, 901, 1623, 2311, 2681
AcoI	YGGCCR	5 cut(s)	434, 1023, 1458, 1476, 1883
AcsI	RAATTY	2 cut(s)	977, 2229
AcuI	CTGAAG	6 cut(s)	759, 1172, 2043, 2156, 2352, 2609
AcvI	CACGTG	1 cut(s)	13
AdeI	CACNNNGTG	3 cut(s)	678, 988, 1577
AfaI	GTAC	9 cut(s)	432, 471, 1077, 1236, 1723, 1893, 2143, 2269, 2362
AfiI	CCNNNNNNNGG	6 cut(s)	1153, 1822, 1982, 2078, 2375, 2577
AgeI	ACCGGT	1 cut(s)	2357
AhlI	ACTAGT	2 cut(s)	1724, 1888
AjnI	CCWGG	1 cut(s)	2318
AjuI	GAANNNNNNNTTGG	4 cut(s)	809, 841, 2701, 2733
Alw21I	GWGCWC	6 cut(s)	18, 630, 911, 963, 2211, 2410
Alw26I	GTCTC	4 cut(s)	765, 1620, 1773, 2779
Alw44I	GTGCAC	1 cut(s)	959
AlwI	GGATC	8 cut(s)	93, 179, 509, 522, 901, 1623, 2311, 2681
Ama87I	CYCGRG	1 cut(s)	125
Aor13HI	TCCGGA	1 cut(s)	2670
AoxI	GGCC	8 cut(s)	128, 434, 1023, 1458, 1476, 1869, 1883, 2794
ApaI	GGGCCC	2 cut(s)	132, 2798
ApaLI	GTGCAC	1 cut(s)	959
ApeKI	GCWGC	6 cut(s)	311, 442, 662, 1252, 2235, 2431
ApoI	RAATTY	2 cut(s)	977, 2229
ArsI	GACNNNNNNTTYG	2 cut(s)	380, 412
AsiGI	ACCGGT	1 cut(s)	2357
Asp700I	GAANNNNTTC	1 cut(s)	1561
Asp718I	GGTACC	1 cut(s)	2360
AspLEI	GCGC	1 cut(s)	331
AsuC2I	CCSGG	3 cut(s)	126, 127, 369
AsuHPI	GGTGA	4 cut(s)	23, 130, 184, 778
AsuII	TTCGAA	1 cut(s)	2245
AvaI	CYCGRG	1 cut(s)	125
AvaII	GGWCC	5 cut(s)	7, 271, 586, 1381, 1637
AxyI	CCTNAGG	1 cut(s)	20
BaeGI	GKGCMC	3 cut(s)	132, 963, 2798
BalI	TGGCCA	3 cut(s)	1025, 1478, 1885
BamHI	GGATCC	1 cut(s)	514
BanI	GGYRCC	2 cut(s)	998, 2360
BanII	GRGCYC	5 cut(s)	132, 361, 555, 630, 2798
BauI	CACGAG	2 cut(s)	698, 983
BbrPI	CACGTG	1 cut(s)	13
BbsI	GAAGAC	1 cut(s)	2613
Bbv12I	GWGCWC	6 cut(s)	18, 630, 911, 963, 2211, 2410
BbvI	GCAGC	6 cut(s)	298, 429, 649, 1239, 2222, 2443
BccI	CCATC	9 cut(s)	152, 203, 1063, 1405, 1487, 1703, 1984, 2247, 2294
BceAI	ACGGC	4 cut(s)	421, 449, 605, 650
BcgI	CGANNNNNNTGC	2 cut(s)	314, 348
BciT130I	CCWGG	1 cut(s)	2320
BcnI	CCSGG	3 cut(s)	126, 127, 369
BcoDI	GTCTC	4 cut(s)	765, 1620, 1773, 2779
BcuI	ACTAGT	2 cut(s)	1724, 1888
BfuAI	ACCTGC	1 cut(s)	1531
BglII	AGATCT	2 cut(s)	1217, 2131
BisI	GCNGC	6 cut(s)	312, 443, 663, 1253, 2236, 2432
BlsI	GCNGC	6 cut(s)	313, 444, 664, 1254, 2237, 2433
BmcAI	AGTACT	1 cut(s)	1236
Bme1390I	CCNGG	4 cut(s)	126, 127, 369, 2320
Bme18I	GGWCC	5 cut(s)	7, 271, 586, 1381, 1637
BmeT110I	CYCGRG	1 cut(s)	125
BmrFI	CCNGG	4 cut(s)	126, 127, 369, 2320
BmsI	GCATC	3 cut(s)	1135, 1276, 1321
BpiI	GAAGAC	1 cut(s)	2613
BplI	GAGNNNNNCTC	2 cut(s)	2759, 2791
BpmI	CTGGAG	3 cut(s)	969, 1068, 1473
Bpu14I	TTCGAA	1 cut(s)	2245
BpuEI	CTTGAG	3 cut(s)	194, 2272, 2411
BpuMI	CCSGG	3 cut(s)	126, 127, 369
BsaAI	YACGTR	2 cut(s)	13, 2461
BsaBI	GATNNNNATC	2 cut(s)	519, 2838
BsaJI	CCNNGG	6 cut(s)	125, 437, 1633, 1784, 1872, 2760
BsaWI	WCCGGW	4 cut(s)	198, 2357, 2508, 2670
BsaXI	ACNNNNNCTCC	6 cut(s)	272, 302, 942, 972, 1406, 1436
Bsc4I	CCNNNNNNNGG	6 cut(s)	1153, 1822, 1982, 2078, 2375, 2577
Bse118I	RCCGGY	4 cut(s)	293, 390, 1460, 2357
Bse1I	ACTGG	3 cut(s)	1026, 1490, 1744
Bse21I	CCTNAGG	1 cut(s)	20
Bse8I	GATNNNNATC	2 cut(s)	519, 2838
BseAI	TCCGGA	1 cut(s)	2670
BseBI	CCWGG	1 cut(s)	2320
BseDI	CCNNGG	6 cut(s)	125, 437, 1633, 1784, 1872, 2760
BseJI	GATNNNNATC	2 cut(s)	519, 2838
BseLI	CCNNNNNNNGG	6 cut(s)	1153, 1822, 1982, 2078, 2375, 2577
BseNI	ACTGG	3 cut(s)	1026, 1490, 1744
BseRI	GAGGAG	5 cut(s)	8, 206, 275, 2183, 2767
BseSI	GKGCMC	3 cut(s)	132, 963, 2798
BseX3I	CGGCCG	1 cut(s)	434
BseXI	GCAGC	6 cut(s)	298, 429, 649, 1239, 2222, 2443
BsgI	GTGCAG	1 cut(s)	1642
Bsh1236I	CGCG	4 cut(s)	183, 235, 306, 329
Bsh1285I	CGRYCG	1 cut(s)	437
BshFI	GGCC	8 cut(s)	130, 436, 1025, 1460, 1478, 1871, 1885, 2796
BshNI	GGYRCC	2 cut(s)	998, 2360
BshTI	ACCGGT	1 cut(s)	2357
BsiEI	CGRYCG	1 cut(s)	437
BsiHKAI	GWGCWC	6 cut(s)	18, 630, 911, 963, 2211, 2410
BsiHKCI	CYCGRG	1 cut(s)	125
BsiSI	CCGG	9 cut(s)	126, 199, 294, 369, 391, 1461, 2358, 2509, 2671
BsiWI	CGTACG	1 cut(s)	430
BslI	CCNNNNNNNGG	6 cut(s)	1153, 1822, 1982, 2078, 2375, 2577
BsmAI	GTCTC	4 cut(s)	765, 1620, 1773, 2779
BsnI	GGCC	8 cut(s)	130, 436, 1025, 1460, 1478, 1871, 1885, 2796
BsoBI	CYCGRG	1 cut(s)	125
Bsp119I	TTCGAA	1 cut(s)	2245
Bsp120I	GGGCCC	2 cut(s)	128, 2794
Bsp13I	TCCGGA	1 cut(s)	2670
Bsp68I	TCGCGA	2 cut(s)	183, 235
BspANI	GGCC	8 cut(s)	130, 436, 1025, 1460, 1478, 1871, 1885, 2796
BspEI	TCCGGA	1 cut(s)	2670
BspFNI	CGCG	4 cut(s)	183, 235, 306, 329
BspMI	ACCTGC	1 cut(s)	1531
BspPI	GGATC	8 cut(s)	93, 179, 509, 522, 901, 1623, 2311, 2681
BspQI	GCTCTTC	1 cut(s)	1938
BspT104I	TTCGAA	1 cut(s)	2245
BspT107I	GGYRCC	2 cut(s)	998, 2360
BsrBI	CCGCTC	1 cut(s)	190
BsrFI	RCCGGY	4 cut(s)	293, 390, 1460, 2357
BsrI	ACTGG	3 cut(s)	1026, 1490, 1744
BssAI	RCCGGY	4 cut(s)	293, 390, 1460, 2357
BssECI	CCNNGG	6 cut(s)	125, 437, 1633, 1784, 1872, 2760
BssSI	CACGAG	2 cut(s)	698, 983
BssT1I	CCWWGG	3 cut(s)	1633, 1784, 1872
Bst2BI	CACGAG	2 cut(s)	698, 983
Bst2UI	CCWGG	1 cut(s)	2320
Bst4CI	ACNGT	4 cut(s)	575, 590, 676, 2599
Bst6I	CTCTTC	2 cut(s)	1938, 2132
BstAPI	GCANNNNNTGC	1 cut(s)	2541
BstBAI	YACGTR	2 cut(s)	13, 2461
BstBI	TTCGAA	1 cut(s)	2245
BstC8I	GCNNGC	6 cut(s)	299, 333, 706, 1462, 1466, 1950
BstDSI	CCRYGG	1 cut(s)	437
BstEII	GGTNACC	2 cut(s)	29, 874
BstENI	CCTNNNNNAGG	1 cut(s)	1151
BstFNI	CGCG	4 cut(s)	183, 235, 306, 329
BstHHI	GCGC	1 cut(s)	331
BstMAI	GTCTC	4 cut(s)	765, 1620, 1773, 2779
BstMCI	CGRYCG	1 cut(s)	437
BstNI	CCWGG	1 cut(s)	2320
BstNSI	RCATGY	1 cut(s)	1174
BstPI	GGTNACC	2 cut(s)	29, 874
BstSCI	CCNGG	4 cut(s)	124, 125, 367, 2318
BstSLI	GKGCMC	3 cut(s)	132, 963, 2798
BstUI	CGCG	4 cut(s)	183, 235, 306, 329
BstV1I	GCAGC	6 cut(s)	298, 429, 649, 1239, 2222, 2443
BstV2I	GAAGAC	1 cut(s)	2613
BstX2I	RGATCY	8 cut(s)	98, 514, 893, 1217, 1628, 2131, 2303, 2673
BstYI	RGATCY	8 cut(s)	98, 514, 893, 1217, 1628, 2131, 2303, 2673
BstZI	CGGCCG	1 cut(s)	434
Bsu36I	CCTNAGG	1 cut(s)	20
BsuRI	GGCC	8 cut(s)	130, 436, 1025, 1460, 1478, 1871, 1885, 2796
BtgI	CCRYGG	1 cut(s)	437
BtsIMutI	CAGTG	5 cut(s)	911, 1019, 1571, 1625, 2067
BtuMI	TCGCGA	2 cut(s)	183, 235
BveI	ACCTGC	1 cut(s)	1531
Cac8I	GCNNGC	6 cut(s)	299, 333, 706, 1462, 1466, 1950
CfoI	GCGC	1 cut(s)	331
Cfr10I	RCCGGY	4 cut(s)	293, 390, 1460, 2357
Cfr9I	CCCGGG	1 cut(s)	125
CpoI	CGGWCCG	1 cut(s)	271
CseI	GACGC	1 cut(s)	335
Csp6I	GTAC	9 cut(s)	431, 470, 1076, 1235, 1722, 1892, 2142, 2268, 2361
CspAI	ACCGGT	1 cut(s)	2357
CspI	CGGWCCG	1 cut(s)	271
CviQI	GTAC	9 cut(s)	431, 470, 1076, 1235, 1722, 1892, 2142, 2268, 2361
DraI	TTTAAA	2 cut(s)	1672, 2643
DraIII	CACNNNGTG	3 cut(s)	678, 988, 1577
EaeI	YGGCCR	5 cut(s)	434, 1023, 1458, 1476, 1883
EagI	CGGCCG	1 cut(s)	434
Eam1104I	CTCTTC	2 cut(s)	1938, 2132
EarI	CTCTTC	2 cut(s)	1938, 2132
EciI	GGCGGA	2 cut(s)	20, 46
Ecl136II	GAGCTC	1 cut(s)	628
EclXI	CGGCCG	1 cut(s)	434
Eco130I	CCWWGG	3 cut(s)	1633, 1784, 1872
Eco24I	GRGCYC	5 cut(s)	132, 361, 555, 630, 2798
Eco32I	GATATC	3 cut(s)	1313, 1879, 2668
Eco47I	GGWCC	5 cut(s)	7, 271, 586, 1381, 1637
Eco52I	CGGCCG	1 cut(s)	434
Eco53kI	GAGCTC	1 cut(s)	628
Eco57I	CTGAAG	6 cut(s)	759, 1172, 2043, 2156, 2352, 2609
Eco72I	CACGTG	1 cut(s)	13
Eco81I	CCTNAGG	1 cut(s)	20
Eco88I	CYCGRG	1 cut(s)	125
Eco91I	GGTNACC	2 cut(s)	29, 874
EcoICRI	GAGCTC	1 cut(s)	628
EcoNI	CCTNNNNNAGG	1 cut(s)	1151
EcoO109I	RGGNCCY	2 cut(s)	2794, 2795
EcoO65I	GGTNACC	2 cut(s)	29, 874
EcoRII	CCWGG	1 cut(s)	2318
EcoRV	GATATC	3 cut(s)	1313, 1879, 2668
EcoT14I	CCWWGG	3 cut(s)	1633, 1784, 1872
EcoT22I	ATGCAT	4 cut(s)	1012, 1128, 1176, 1336
EcoT38I	GRGCYC	5 cut(s)	132, 361, 555, 630, 2798
ErhI	CCWWGG	3 cut(s)	1633, 1784, 1872
FalI	AAGNNNNNCTT	4 cut(s)	1424, 1456, 1652, 1684
FauI	CCCGC	4 cut(s)	311, 1669, 1975, 2399
FauNDI	CATATG	2 cut(s)	1176, 1642
Fnu4HI	GCNGC	6 cut(s)	312, 443, 663, 1253, 2236, 2432
FriOI	GRGCYC	5 cut(s)	132, 361, 555, 630, 2798
Fsp4HI	GCNGC	6 cut(s)	312, 443, 663, 1253, 2236, 2432
GlaI	GCGC	1 cut(s)	330
GluI	GCNGC	6 cut(s)	312, 443, 663, 1253, 2236, 2432
GsuI	CTGGAG	3 cut(s)	969, 1068, 1473
HaeIII	GGCC	8 cut(s)	130, 436, 1025, 1460, 1478, 1871, 1885, 2796
HapII	CCGG	9 cut(s)	126, 199, 294, 369, 391, 1461, 2358, 2509, 2671
HgaI	GACGC	1 cut(s)	335
HhaI	GCGC	1 cut(s)	331
Hin6I	GCGC	1 cut(s)	329
HinP1I	GCGC	1 cut(s)	329
HincII	GTYRAC	3 cut(s)	112, 1198, 1278
HindII	GTYRAC	3 cut(s)	112, 1198, 1278
HindIII	AAGCTT	3 cut(s)	798, 1110, 2416
HinfI	GANTC	7 cut(s)	266, 317, 1088, 1902, 1967, 2285, 2615
HpaI	GTTAAC	1 cut(s)	1278
HpaII	CCGG	9 cut(s)	126, 199, 294, 369, 391, 1461, 2358, 2509, 2671
HphI	GGTGA	4 cut(s)	23, 130, 184, 778
Hpy166II	GTNNAC	9 cut(s)	112, 204, 489, 507, 961, 1198, 1278, 2464, 2690
Hpy8I	GTNNAC	9 cut(s)	112, 204, 489, 507, 961, 1198, 1278, 2464, 2690
Hpy99I	CGWCG	3 cut(s)	65, 329, 2615
HpyCH4III	ACNGT	4 cut(s)	575, 590, 676, 2599
HpyCH4IV	ACGT	7 cut(s)	12, 1504, 1547, 2460, 2562, 2681, 2813
HpySE526I	ACGT	7 cut(s)	12, 1504, 1547, 2460, 2562, 2681, 2813
HspAI	GCGC	1 cut(s)	329
Kpn2I	TCCGGA	1 cut(s)	2670
KpnI	GGTACC	1 cut(s)	2364
KroI	GCCGGC	1 cut(s)	1460
KroNI	GCCGGC	1 cut(s)	1462
KspAI	GTTAAC	1 cut(s)	1278
LguI	GCTCTTC	1 cut(s)	1938
Lsp1109I	GCAGC	6 cut(s)	298, 429, 649, 1239, 2222, 2443
LweI	GCATC	3 cut(s)	1135, 1276, 1321
MaeII	ACGT	7 cut(s)	12, 1504, 1547, 2460, 2562, 2681, 2813
MbiI	CCGCTC	1 cut(s)	190
MfeI	CAATTG	1 cut(s)	1130
MflI	RGATCY	8 cut(s)	98, 514, 893, 1217, 1628, 2131, 2303, 2673
MlsI	TGGCCA	3 cut(s)	1025, 1478, 1885
MluCI	AATT	9 cut(s)	51, 372, 380, 977, 1130, 1761, 2090, 2229, 2727
MluNI	TGGCCA	3 cut(s)	1025, 1478, 1885
MlyI	GAGTC	4 cut(s)	260, 311, 1082, 1961
MmeI	TCCRAC	3 cut(s)	246, 525, 1416
Mox20I	TGGCCA	3 cut(s)	1025, 1478, 1885
Mph1103I	ATGCAT	4 cut(s)	1012, 1128, 1176, 1336
MroI	TCCGGA	1 cut(s)	2670
MroNI	GCCGGC	1 cut(s)	1460
MroXI	GAANNNNTTC	1 cut(s)	1561
MscI	TGGCCA	3 cut(s)	1025, 1478, 1885
MseI	TTAA	7 cut(s)	1058, 1262, 1277, 1671, 2105, 2642, 2730
MslI	CAYNNNNRTG	2 cut(s)	779, 1175
Msp20I	TGGCCA	3 cut(s)	1025, 1478, 1885
MspA1I	CMGCKG	1 cut(s)	1748
MspI	CCGG	9 cut(s)	126, 199, 294, 369, 391, 1461, 2358, 2509, 2671
MspR9I	CCNGG	4 cut(s)	126, 127, 369, 2320
MunI	CAATTG	1 cut(s)	1130
MvaI	CCWGG	1 cut(s)	2320
MvnI	CGCG	4 cut(s)	183, 235, 306, 329
NaeI	GCCGGC	1 cut(s)	1462
NciI	CCSGG	3 cut(s)	126, 127, 369
NdeI	CATATG	2 cut(s)	1176, 1642
NgoMIV	GCCGGC	1 cut(s)	1460
NmeAIII	GCCGAG	1 cut(s)	481
NmuCI	GTSAC	5 cut(s)	29, 590, 1837, 2197, 2274
NruI	TCGCGA	2 cut(s)	183, 235
NsiI	ATGCAT	4 cut(s)	1012, 1128, 1176, 1336
NspI	RCATGY	1 cut(s)	1174
NspV	TTCGAA	1 cut(s)	2245
PaqCI	CACCTGC	1 cut(s)	1531
PciSI	GCTCTTC	1 cut(s)	1938
PcsI	WCGNNNNNNNCGW	3 cut(s)	579, 2568, 2658
PdiI	GCCGGC	1 cut(s)	1462
PdmI	GAANNNNTTC	1 cut(s)	1561
PfeI	GAWTC	3 cut(s)	1902, 2285, 2615
Pfl23II	CGTACG	1 cut(s)	430
PflFI	GACNNNGTC	1 cut(s)	269
PfoI	TCCNGGA	1 cut(s)	367
PinAI	ACCGGT	1 cut(s)	2357
PkrI	GCNGC	6 cut(s)	313, 444, 664, 1254, 2237, 2433
PleI	GAGTC	4 cut(s)	260, 311, 1082, 1961
PmaCI	CACGTG	1 cut(s)	13
PmlI	CACGTG	1 cut(s)	13
PpsI	GAGTC	4 cut(s)	260, 311, 1082, 1961
Ppu21I	YACGTR	2 cut(s)	13, 2461
PsiI	TTATAA	2 cut(s)	2150, 2327
Psp124BI	GAGCTC	1 cut(s)	630
Psp1406I	AACGTT	1 cut(s)	2562
Psp6I	CCWGG	1 cut(s)	2318
PspCI	CACGTG	1 cut(s)	13
PspEI	GGTNACC	2 cut(s)	29, 874
PspGI	CCWGG	1 cut(s)	2318
PspLI	CGTACG	1 cut(s)	430
PspOMI	GGGCCC	2 cut(s)	128, 2794
PsuI	RGATCY	8 cut(s)	98, 514, 893, 1217, 1628, 2131, 2303, 2673
PsyI	GACNNNGTC	1 cut(s)	269
PvuII	CAGCTG	1 cut(s)	1748
RruI	TCGCGA	2 cut(s)	183, 235
RsaI	GTAC	9 cut(s)	432, 471, 1077, 1236, 1723, 1893, 2143, 2269, 2362
RsaNI	GTAC	9 cut(s)	431, 470, 1076, 1235, 1722, 1892, 2142, 2268, 2361
RseI	CAYNNNNRTG	2 cut(s)	779, 1175
Rsr2I	CGGWCCG	1 cut(s)	271
RsrII	CGGWCCG	1 cut(s)	271
SacI	GAGCTC	1 cut(s)	630
SapI	GCTCTTC	1 cut(s)	1938
SaqAI	TTAA	7 cut(s)	1058, 1262, 1277, 1671, 2105, 2642, 2730
SatI	GCNGC	6 cut(s)	312, 443, 663, 1253, 2236, 2432
ScaI	AGTACT	1 cut(s)	1236
SchI	GAGTC	4 cut(s)	260, 311, 1082, 1961
ScrFI	CCNGG	4 cut(s)	126, 127, 369, 2320
SfaNI	GCATC	3 cut(s)	1135, 1276, 1321
SfuI	TTCGAA	1 cut(s)	2245
SinI	GGWCC	5 cut(s)	7, 271, 586, 1381, 1637
SmaI	CCCGGG	1 cut(s)	127
SmiMI	CAYNNNNRTG	2 cut(s)	779, 1175
SmlI	CTYRAG	3 cut(s)	209, 2251, 2426
SmoI	CTYRAG	3 cut(s)	209, 2251, 2426
SpeI	ACTAGT	2 cut(s)	1724, 1888
Sse9I	AATT	9 cut(s)	51, 372, 380, 977, 1130, 1761, 2090, 2229, 2727
SspI	AATATT	2 cut(s)	1516, 1530
SstI	GAGCTC	1 cut(s)	630
StyD4I	CCNGG	4 cut(s)	124, 125, 367, 2318
StyI	CCWWGG	3 cut(s)	1633, 1784, 1872
TaaI	ACNGT	4 cut(s)	575, 590, 676, 2599
TaiI	ACGT	7 cut(s)	15, 1507, 1550, 2463, 2565, 2684, 2816
TaqII	GACCGA	1 cut(s)	259
TasI	AATT	9 cut(s)	51, 372, 380, 977, 1130, 1761, 2090, 2229, 2727
TatI	WGTACW	5 cut(s)	1075, 1234, 1721, 1891, 2141
TfiI	GAWTC	3 cut(s)	1902, 2285, 2615
Tru1I	TTAA	7 cut(s)	1058, 1262, 1277, 1671, 2105, 2642, 2730
Tru9I	TTAA	7 cut(s)	1058, 1262, 1277, 1671, 2105, 2642, 2730
TscAI	CASTG	5 cut(s)	911, 1026, 1578, 1625, 2067
TseFI	GTSAC	5 cut(s)	29, 590, 1837, 2197, 2274
TseI	GCWGC	6 cut(s)	311, 442, 662, 1252, 2235, 2431
Tsp45I	GTSAC	5 cut(s)	29, 590, 1837, 2197, 2274
TspGWI	ACGGA	4 cut(s)	263, 408, 418, 978
TspMI	CCCGGG	1 cut(s)	125
TspRI	CASTG	5 cut(s)	911, 1026, 1578, 1625, 2067
Tth111I	GACNNNGTC	1 cut(s)	269
VneI	GTGCAC	1 cut(s)	959
VpaK11BI	GGWCC	5 cut(s)	7, 271, 586, 1381, 1637
XagI	CCTNNNNNAGG	1 cut(s)	1151
XapI	RAATTY	2 cut(s)	977, 2229
XbaI	TCTAGA	1 cut(s)	1294
XceI	RCATGY	1 cut(s)	1174
XmaI	CCCGGG	1 cut(s)	125
XmnI	GAANNNNTTC	1 cut(s)	1561
ZrmI	AGTACT	1 cut(s)	1236
Zsp2I	ATGCAT	4 cut(s)	1012, 1128, 1176, 1336

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.