Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0004412

Physical Location & Seq

Genomic Coordinates Forward (+)

40006068 .. 40010851

Gene Structure 4784 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00418480.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2865 bp

ATGGAGGAGGACCACGCGCCCGGCGCGTGCAATCACTCTCTCAACCTGCCATCAATTTCCCCCACCGAACCCCTCTCCACCGAAACCCTAGCTTCCCTGAGATCCCTAATCATTAACCCCTCAACTCCCTCCCCCGCAATTTCTTCAATCATCGAAACCCTAACTCGCTCTCTCCAACTCAGTCGCGACCCGCTCCGCACTCTCAAGCTCCTCTCCGACCTGGCCGCCCGACACCCCCACCTCTCCGCCCTCGTCTTCCACTCCGTCCGCTCCGACTCGCTCCTCTCCACCGAGTCGACTCGCGTCGCCGCCGAGTCACTCGATGTCTTGGCTTCGATCGCAGAGCGGAATCGAGAGCTAGCGCCGGCTGTTGAGGAAATCGACGACCGGTTGTTGGCCTCGCTTTGTTTTAGTCCCTCTCCGGCGATACGGCCGTGGCTTCTCAGAAACGCCGAGAGGTTTGGTGTTCAGCCTTACTTGCTATCAAGCCTGTTTCTAGGGTTTACGAAGGATCCGTATCCGAATGTGAGGAAAGCTGCATTGGATGGACTCGTTGGTTTGAGTAAGAGCGGCGTTATTGACGACGGTGATATGATCAGAGGCTGCTACTTCCGCGCCGGTGAGCTTCTAAACGACATGGAAGACGGCGTTAGGTCCGCTGCAATTCGAGCCGTGTTTGCTTGGGGTCTAATCCTCATGGTGTGCGATTCGGAGGCGAAAGCATACTGGTCTGATGAAGTATTTGTCAAGATTTGTTCGATGGTAAGAGACATGAGCATGGAGGTCAGGATTGAAGCTTTTCATGCCCTTGGGAAGATAGGAATGGTATCTGAAGATATTCTTTTGCAAACCCTGTCAAAGAAAGTCCTGGTGGCTACTAAAGAAAAGAAATCTCTTGCTCAGTGTTCCGATGAACAACTGGAAGGATCAGGGTCTAGTGTTGCAGGAGCTTTTATGCATGGACTTGAGGATGAATTCCATGAGGTATCTTTTGACTCTGACATGCAACGAACTTTTACCAAAAGCGCGCTTTTGAATAATGAACTCTTTTTTATCAATATGAAGGTACGAAAAGCTGCATGTCATTCTTTGGGTACACTCACCATACTCTCTTCCAAGTTTGCTGGAGAAGCCTTGAACTTACTGATGGATGTACTGAATGATGACTCAATGCTTGTGCGGTTACAAGCTTTTGAAACTATGCATCAAATGGCAACTTTTGGTCATCTGAAGGTGCAAGAAAAACACATGCATATGTTTCTTGGCACTCTGGTTGACCGTAATACCCTGACAAGATCTGCTGCAAGGAAAGTACTTAAATTGGCAAAACTGCCAGATTTGAAGTTGTTTAGATCAACTATTGATACCCTTTTAGATAACTTGGAAATCTGTCCACAGGATGAAGCAGATATATTAAATGTTTTGTTTCATATTGGACGAAATCATGGAAAGTTTGTGGTCCGCATTATTGAGGAAGTTTCTCAGCAGATGGAGCCAGTGTCTAATGGCAAGTTAGGCTTTGATAACGTGAGGGTGGGCGCCTTGCTAGTTCTGGCCATCTCAGCTCCACTTTCACAAGAACGTGATTGCAGTATCCCACCTAGTATATTTTCTTATGCAGTTACATACCTGGGAAGGATTTCTCATGCTTTGAGTGACATTATTCATCAAGAGGGCCTGTTGAATTACTTGTCTCAATGCAGTAGATCCTCTGGACCGTATGATATGGAGTTTGACTTCAGCGCGAAAAGAGGTGGAACCTCTGAAATCTTGTCTCCAACAGTTAAGAAACCAAGGGAGGCTGGAAATTCTCTTGTAGAGTACAGTCTAGATGTGCAAGATGAAGTGACCAATTCAATGAATGTGGTTCTTGAAAAGGTGAAGGATATCTGGCCATTTGTACAAGCTGGATTCACGACTGAAGGACTCAGGACTTTAAGAAGTTGCAGGGAAGAATTGGCAACATTGACGTCTGGCTCTATTGCATCTGGTGGTATCTTTGCCTTTACGATGCAGTATATCAAGATCACGAAACTGCTTGCAAAGGCATGGGTGAACTTCGTGCCTTCAAGACACCTTCCTTCTAGCGGGATGGGAGACTTGGACTTTGTGTTGGGAAAGCTAGACGCGAGGCTTAGTGAACTGAAAAGTACGTTTATAGGATTGTCTAAAGTGGAAGAGTTGCATATTTTGGAGCTGGTACTTGTGACTTGTATGCTCAAGCTTTCTAAAGTTGAAATTTGCTGCCATCTTGGTACTTTGAGAAAGCTCTCTTGTACAATGGCACATGTGGAATCTCTCCTTGAAGATGGAACTGTTGAACCATCCAGGTTTATGATTGAAGTCAGAAAATTATTATCTGAAATTGGGACGTCTCTCAATGGTAGTTCCTGTAATCCAATTCTTTTTAGAAGAGTACTGGAAAGTTTCTTCCTTGAGCGGCTTGTGTTATGTGGTGGACTAAAGCACATAAAGGCAGATCTGGACATACCTGACAATGACTACGAACATCCTCTTTGCTTTGTCACAGGGTTGCCCGTTGGTATACCATGCCACATCACTCTTCATAATGTTTCGGTTGAAAGCAGATTATGGCTCAAAATGACTGCCAATGGAATTGATGATACTCAGTTTGTCTCCTTGGATTCAAACCTTTTTGGAGGCTGCGATGATCATAGAATAGTTGTGTTTACCGCACCATTTTACAAAACCCCGAAGGCATTTTCTTTTACAGTCAGCGTTTGCATTTGTATGGAATATTTGTCTGAGGATGAGGATGTGTCTTTTCTCAAACGTTGTGGACCTAGACATGAGCTTACATCTCTTTGCCGAGAAAAGGAAGTTTTTCTTTCTATGGTCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006810 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009956 GO:0009987 GO:0010467 GO:0010496 GO:0012505 GO:0016070 GO:0016073 GO:0016180 GO:0022622 GO:0031123 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0034470 GO:0034472 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043628 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048364 GO:0048731 GO:0048856 GO:0051179 GO:0051234 GO:0071704 GO:0090057 GO:0090304 GO:0097708 GO:0099402 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

954

Amino Acids

105.69

Weight (kDa)

5.75

Isoelectric Point (pI)

45.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
INTS4_C	PF25458	830 - 950	1.4e-09	Integrator subunit 4 family C-terminal Ig-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004412)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08800 AT3G08800 AT3G08800
fragaria_vesca	FvH4_6g10600 FvH4_6g10600 FvH4_6g10600 FvH4_6g10600 FvH4_6g10600
malus_domestica	MD04G1166800.v1.1
prunus_persica	Prupe.6G285000_v2.0.a1 Prupe.6G285000_v2.0.a1
pyrus_communis	pycom01g05880 pycom04g14820
rosa_chinensis	RchiOBHm_Chr3g0461591 RchiOBHm_Chr3g0461601
rosa_laevigata	RLG00000024915
rosa_multiflora	Rmu_co8098260.1_g000001 Rmu_sc0003578.1_g000001 Rmu_sc0032177.1_g000001 Rmu_sc0040016.1_g000001 Rmu_ssc0000193.1_g000007
rosa_roxburghii	Rroxscaffold_6G00418110 Rroxscaffold_6G00418480
rosa_rugosa	Rorug03G0052200 Rorug03G0052200 Rorug03G0052300 Rorug03G0052300
rosa_samantha	Rh3AG110400 Rh3BG113600 Rh3CG115500 Rh3DG115300
rosa_wichuraiana	Rw3G009340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	1973, 2375
Acc36I	ACCTGC	1 cut(s)	54
AccB1I	GGYRCC	1 cut(s)	1538
AccBSI	CCGCTC	5 cut(s)	193, 270, 346, 570, 2440
AccI	GTMKAC	2 cut(s)	296, 2545
AccII	CGCG	8 cut(s)	17, 26, 186, 303, 615, 1028, 1745, 2129
AclI	AACGTT	1 cut(s)	2794
AclWI	GGATC	5 cut(s)	96, 506, 519, 934, 1701
AcoI	YGGCCR	4 cut(s)	222, 431, 1554, 1892
AcsI	RAATTY	3 cut(s)	974, 1807, 2238
AcuI	CTGAAG	4 cut(s)	852, 1250, 1723, 1941
AcyI	GRCGYC	3 cut(s)	1539, 1970, 2372
AfiI	CCNNNNNNNGG	3 cut(s)	394, 2087, 2836
AflIII	ACRYGT	1 cut(s)	2287
AgeI	ACCGGT	1 cut(s)	387
AjnI	CCWGG	4 cut(s)	219, 867, 1629, 2327
AjuI	GAANNNNNNNTTGG	2 cut(s)	524, 556
Alw26I	GTCTC	6 cut(s)	762, 1698, 1779, 2091, 2379, 2641
AlwI	GGATC	5 cut(s)	96, 506, 519, 934, 1701
AlwNI	CAGNNNCTG	1 cut(s)	603
AoxI	GGCC	6 cut(s)	222, 396, 431, 1554, 1675, 1892
ApeKI	GCWGC	7 cut(s)	536, 603, 659, 1076, 1301, 2244, 2664
ApoI	RAATTY	3 cut(s)	974, 1807, 2238
ArsI	GACNNNNNNTTYG	2 cut(s)	2090, 2122
AsiGI	ACCGGT	1 cut(s)	387
Asp700I	GAANNNNTTC	4 cut(s)	798, 837, 1639, 2844
AspLEI	GCGC	8 cut(s)	19, 26, 364, 617, 1028, 1030, 1541, 1745
AspS9I	GGNCC	6 cut(s)	10, 654, 1459, 1675, 1715, 2801
AsuC2I	CCSGG	1 cut(s)	21
AsuHPI	GGTGA	5 cut(s)	599, 632, 1093, 1891, 2065
AsuNHI	GCTAGC	1 cut(s)	358
AvaII	GGWCC	5 cut(s)	10, 654, 1459, 1715, 2801
BaeI	ACNNNNGTAYC	2 cut(s)	419, 452
BalI	TGGCCA	2 cut(s)	1556, 1894
BamHI	GGATCC	1 cut(s)	511
BanI	GGYRCC	1 cut(s)	1538
BbsI	GAAGAC	2 cut(s)	247, 648
BbvI	GCAGC	7 cut(s)	523, 590, 646, 1063, 1288, 2231, 2651
BceAI	ACGGC	4 cut(s)	418, 446, 656, 661
BciT130I	CCWGG	4 cut(s)	221, 869, 1631, 2329
BciVI	GTATCC	2 cut(s)	528, 1604
BclI	TGATCA	2 cut(s)	594, 2671
BcnI	CCSGG	1 cut(s)	21
BcoDI	GTCTC	6 cut(s)	762, 1698, 1779, 2091, 2379, 2641
BfoI	RGCGCY	2 cut(s)	365, 1542
BfuAI	ACCTGC	1 cut(s)	54
BfuI	GTATCC	2 cut(s)	528, 1604
BglII	AGATCT	2 cut(s)	1295, 2479
BmcAI	AGTACT	2 cut(s)	1314, 2418
Bme1390I	CCNGG	5 cut(s)	21, 221, 869, 1631, 2329
Bme18I	GGWCC	5 cut(s)	10, 654, 1459, 1715, 2801
BmgT120I	GGNCC	6 cut(s)	10, 654, 1459, 1675, 1715, 2801
BmiI	GGNNCC	4 cut(s)	513, 1494, 1540, 1759
BmrFI	CCNGG	5 cut(s)	21, 221, 869, 1631, 2329
BmsI	GCATC	3 cut(s)	1213, 1994, 2001
BmtI	GCTAGC	1 cut(s)	362
BoxI	GACNNNNGTC	1 cut(s)	302
BpiI	GAAGAC	2 cut(s)	247, 648
BpmI	CTGGAG	1 cut(s)	1146
BpuEI	CTTGAG	4 cut(s)	188, 986, 2204, 2456
BpuMI	CCSGG	1 cut(s)	21
BsaBI	GATNNNNATC	1 cut(s)	516
BsaHI	GRCGYC	3 cut(s)	1539, 1970, 2372
BsaJI	CCNNGG	5 cut(s)	434, 808, 1630, 1793, 2640
BsaWI	WCCGGW	1 cut(s)	387
BsaXI	ACNNNNNCTCC	2 cut(s)	1484, 1514
Bsc4I	CCNNNNNNNGG	3 cut(s)	394, 2087, 2836
Bse118I	RCCGGY	3 cut(s)	364, 387, 617
Bse1I	ACTGG	4 cut(s)	731, 924, 1496, 2424
Bse8I	GATNNNNATC	1 cut(s)	516
BseBI	CCWGG	4 cut(s)	221, 869, 1631, 2329
BseDI	CCNNGG	5 cut(s)	434, 808, 1630, 1793, 2640
BseGI	GGATG	9 cut(s)	550, 976, 1156, 1405, 2097, 2324, 2509, 2776, 2782
BseJI	GATNNNNATC	1 cut(s)	516
BseLI	CCNNNNNNNGG	3 cut(s)	394, 2087, 2836
BseMII	CTCAG	9 cut(s)	89, 193, 457, 914, 1496, 1575, 1942, 2642, 2757
BseNI	ACTGG	4 cut(s)	731, 924, 1496, 2424
BsePI	GCGCGC	1 cut(s)	1026
BseRI	GAGGAG	3 cut(s)	20, 200, 272
BseX3I	CGGCCG	1 cut(s)	431
BseXI	GCAGC	7 cut(s)	523, 590, 646, 1063, 1288, 2231, 2651
Bsh1236I	CGCG	8 cut(s)	17, 26, 186, 303, 615, 1028, 1745, 2129
Bsh1285I	CGRYCG	3 cut(s)	339, 388, 434
BshFI	GGCC	6 cut(s)	224, 398, 433, 1556, 1677, 1894
BshNI	GGYRCC	1 cut(s)	1538
BshTI	ACCGGT	1 cut(s)	387
BsiEI	CGRYCG	3 cut(s)	339, 388, 434
BsiSI	CCGG	5 cut(s)	21, 365, 388, 422, 618
BslFI	GGGAC	2 cut(s)	399, 2383
BslI	CCNNNNNNNGG	3 cut(s)	394, 2087, 2836
BsmAI	GTCTC	6 cut(s)	762, 1698, 1779, 2091, 2379, 2641
BsmBI	CGTCTC	1 cut(s)	2379
BsmFI	GGGAC	2 cut(s)	399, 2383
BsnI	GGCC	6 cut(s)	224, 398, 433, 1556, 1677, 1894
Bsp1407I	TGTACA	2 cut(s)	1900, 2276
Bsp68I	TCGCGA	1 cut(s)	186
BspANI	GGCC	6 cut(s)	224, 398, 433, 1556, 1677, 1894
BspCNI	CTCAG	9 cut(s)	90, 192, 456, 913, 1495, 1574, 1941, 2641, 2758
BspFNI	CGCG	8 cut(s)	17, 26, 186, 303, 615, 1028, 1745, 2129
BspLI	GGNNCC	4 cut(s)	513, 1494, 1540, 1759
BspMI	ACCTGC	1 cut(s)	54
BspOI	GCTAGC	1 cut(s)	362
BspPI	GGATC	5 cut(s)	96, 506, 519, 934, 1701
BspT107I	GGYRCC	1 cut(s)	1538
BsrBI	CCGCTC	5 cut(s)	193, 270, 346, 570, 2440
BsrFI	RCCGGY	3 cut(s)	364, 387, 617
BsrGI	TGTACA	2 cut(s)	1900, 2276
BsrI	ACTGG	4 cut(s)	731, 924, 1496, 2424
BssAI	RCCGGY	3 cut(s)	364, 387, 617
BssECI	CCNNGG	5 cut(s)	434, 808, 1630, 1793, 2640
BssHII	GCGCGC	1 cut(s)	1026
BssNAI	GTATAC	1 cut(s)	2546
BssNI	GRCGYC	3 cut(s)	1539, 1970, 2372
BssT1I	CCWWGG	3 cut(s)	808, 1793, 2640
Bst1107I	GTATAC	1 cut(s)	2546
Bst2UI	CCWGG	4 cut(s)	221, 869, 1631, 2329
Bst4CI	ACNGT	7 cut(s)	587, 1280, 1719, 1783, 1826, 2317, 2734
Bst6I	CTCTTC	4 cut(s)	1117, 2172, 2407, 2568
BstACI	GRCGYC	3 cut(s)	1539, 1970, 2372
BstAUI	TGTACA	2 cut(s)	1900, 2276
BstC8I	GCNNGC	5 cut(s)	28, 360, 366, 1028, 2040
BstDSI	CCRYGG	1 cut(s)	434
BstF5I	GGATG	9 cut(s)	550, 976, 1156, 1405, 2097, 2324, 2509, 2776, 2782
BstFNI	CGCG	8 cut(s)	17, 26, 186, 303, 615, 1028, 1745, 2129
BstH2I	RGCGCY	2 cut(s)	365, 1542
BstHHI	GCGC	8 cut(s)	19, 26, 364, 617, 1028, 1030, 1541, 1745
BstMAI	GTCTC	6 cut(s)	762, 1698, 1779, 2091, 2379, 2641
BstMCI	CGRYCG	3 cut(s)	339, 388, 434
BstNI	CCWGG	4 cut(s)	221, 869, 1631, 2329
BstNSI	RCATGY	4 cut(s)	1006, 1083, 1252, 2291
BstPAI	GACNNNNGTC	1 cut(s)	302
BstSCI	CCNGG	5 cut(s)	19, 219, 867, 1629, 2327
BstUI	CGCG	8 cut(s)	17, 26, 186, 303, 615, 1028, 1745, 2129
BstV1I	GCAGC	7 cut(s)	523, 590, 646, 1063, 1288, 2231, 2651
BstV2I	GAAGAC	2 cut(s)	247, 648
BstX2I	RGATCY	5 cut(s)	101, 511, 1295, 1706, 2479
BstYI	RGATCY	5 cut(s)	101, 511, 1295, 1706, 2479
BstZ17I	GTATAC	1 cut(s)	2546
BstZI	CGGCCG	1 cut(s)	431
BsuI	GTATCC	2 cut(s)	528, 1604
BsuRI	GGCC	6 cut(s)	224, 398, 433, 1556, 1677, 1894
BtgI	CCRYGG	1 cut(s)	434
BtgZI	GCGATG	1 cut(s)	2682
BtsCI	GGATG	9 cut(s)	550, 976, 1156, 1405, 2097, 2324, 2509, 2776, 2782
BtsIMutI	CAGTG	2 cut(s)	908, 1503
BtuMI	TCGCGA	1 cut(s)	186
BveI	ACCTGC	1 cut(s)	54
Cac8I	GCNNGC	5 cut(s)	28, 360, 366, 1028, 2040
CaiI	CAGNNNCTG	1 cut(s)	603
CfoI	GCGC	8 cut(s)	19, 26, 364, 617, 1028, 1030, 1541, 1745
Cfr10I	RCCGGY	3 cut(s)	364, 387, 617
Cfr13I	GGNCC	6 cut(s)	10, 654, 1459, 1675, 1715, 2801
CseI	GACGC	2 cut(s)	292, 2135
CspAI	ACCGGT	1 cut(s)	387
CspCI	CAANNNNNGTGG	4 cut(s)	1845, 1880, 2779, 2814
DinI	GGCGCC	1 cut(s)	1540
EaeI	YGGCCR	4 cut(s)	222, 431, 1554, 1892
EagI	CGGCCG	1 cut(s)	431
Eam1104I	CTCTTC	4 cut(s)	1117, 2172, 2407, 2568
EarI	CTCTTC	4 cut(s)	1117, 2172, 2407, 2568
EciI	GGCGGA	1 cut(s)	235
EclXI	CGGCCG	1 cut(s)	431
Eco130I	CCWWGG	3 cut(s)	808, 1793, 2640
Eco32I	GATATC	1 cut(s)	1888
Eco47I	GGWCC	5 cut(s)	10, 654, 1459, 1715, 2801
Eco52I	CGGCCG	1 cut(s)	431
Eco57I	CTGAAG	4 cut(s)	852, 1250, 1723, 1941
EcoO109I	RGGNCCY	1 cut(s)	1675
EcoRI	GAATTC	1 cut(s)	974
EcoRII	CCWGG	4 cut(s)	219, 867, 1629, 2327
EcoRV	GATATC	1 cut(s)	1888
EcoT14I	CCWWGG	3 cut(s)	808, 1793, 2640
EcoT22I	ATGCAT	3 cut(s)	960, 1206, 1254
EgeI	GGCGCC	1 cut(s)	1540
EheI	GGCGCC	1 cut(s)	1540
ErhI	CCWWGG	3 cut(s)	808, 1793, 2640
Esp3I	CGTCTC	1 cut(s)	2379
FalI	AAGNNNNNCTT	6 cut(s)	825, 857, 1502, 1534, 2832, 2864
FaqI	GGGAC	2 cut(s)	399, 2383
FauI	CCCGC	3 cut(s)	142, 198, 2081
FauNDI	CATATG	1 cut(s)	1254
FbaI	TGATCA	2 cut(s)	594, 2671
FblI	GTMKAC	2 cut(s)	296, 2545
FokI	GGATG	9 cut(s)	557, 983, 1163, 1412, 2104, 2311, 2496, 2783, 2789
GlaI	GCGC	8 cut(s)	18, 25, 363, 616, 1027, 1029, 1540, 1744
GsuI	CTGGAG	1 cut(s)	1146
HaeII	RGCGCY	2 cut(s)	365, 1542
HaeIII	GGCC	6 cut(s)	224, 398, 433, 1556, 1677, 1894
HapII	CCGG	5 cut(s)	21, 365, 388, 422, 618
HgaI	GACGC	2 cut(s)	292, 2135
HhaI	GCGC	8 cut(s)	19, 26, 364, 617, 1028, 1030, 1541, 1745
Hin1I	GRCGYC	3 cut(s)	1539, 1970, 2372
Hin6I	GCGC	8 cut(s)	17, 24, 362, 615, 1026, 1028, 1539, 1743
HinP1I	GCGC	8 cut(s)	17, 24, 362, 615, 1026, 1028, 1539, 1743
HincII	GTYRAC	2 cut(s)	297, 1276
HindII	GTYRAC	2 cut(s)	297, 1276
HindIII	AAGCTT	3 cut(s)	795, 1188, 2222
HpaII	CCGG	5 cut(s)	21, 365, 388, 422, 618
HphI	GGTGA	5 cut(s)	599, 632, 1093, 1891, 2065
Hpy99I	CGWCG	3 cut(s)	308, 386, 587
HpyCH4III	ACNGT	7 cut(s)	587, 1280, 1719, 1783, 1826, 2317, 2734
HpyCH4IV	ACGT	6 cut(s)	1527, 1582, 1970, 2153, 2372, 2794
HpySE526I	ACGT	6 cut(s)	1527, 1582, 1970, 2153, 2372, 2794
Hsp92I	GRCGYC	3 cut(s)	1539, 1970, 2372
HspAI	GCGC	8 cut(s)	17, 24, 362, 615, 1026, 1028, 1539, 1743
KasI	GGCGCC	1 cut(s)	1538
KroI	GCCGGC	1 cut(s)	364
KroNI	GCCGGC	1 cut(s)	366
Ksp22I	TGATCA	2 cut(s)	594, 2671
LmnI	GCTCC	8 cut(s)	198, 213, 275, 285, 947, 1492, 1570, 2194
Lsp1109I	GCAGC	7 cut(s)	523, 590, 646, 1063, 1288, 2231, 2651
LweI	GCATC	3 cut(s)	1213, 1994, 2001
MaeII	ACGT	6 cut(s)	1527, 1582, 1970, 2153, 2372, 2794
MaeIII	GTNAC	7 cut(s)	315, 1182, 1621, 1655, 1846, 2206, 2524
MbiI	CCGCTC	5 cut(s)	193, 270, 346, 570, 2440
MflI	RGATCY	5 cut(s)	101, 511, 1295, 1706, 2479
MlsI	TGGCCA	2 cut(s)	1556, 1894
MluNI	TGGCCA	2 cut(s)	1556, 1894
Mly113I	GGCGCC	1 cut(s)	1539
MlyI	GAGTC	8 cut(s)	269, 292, 302, 323, 543, 989, 1160, 1920
MmeI	TCCRAC	4 cut(s)	199, 240, 297, 1802
Mox20I	TGGCCA	2 cut(s)	1556, 1894
Mph1103I	ATGCAT	3 cut(s)	960, 1206, 1254
MroNI	GCCGGC	1 cut(s)	364
MroXI	GAANNNNTTC	4 cut(s)	798, 837, 1639, 2844
MscI	TGGCCA	2 cut(s)	1556, 1894
MseI	TTAA	5 cut(s)	114, 1317, 1415, 1785, 1937
MslI	CAYNNNNRTG	3 cut(s)	776, 1253, 2750
Msp20I	TGGCCA	2 cut(s)	1556, 1894
MspA1I	CMGCKG	1 cut(s)	659
MspI	CCGG	5 cut(s)	21, 365, 388, 422, 618
MspR9I	CCNGG	5 cut(s)	21, 221, 869, 1631, 2329
MvaI	CCWGG	4 cut(s)	221, 869, 1631, 2329
MvnI	CGCG	8 cut(s)	17, 26, 186, 303, 615, 1028, 1745, 2129
NaeI	GCCGGC	1 cut(s)	366
NarI	GGCGCC	1 cut(s)	1539
NciI	CCSGG	1 cut(s)	21
NdeI	CATATG	1 cut(s)	1254
NgoMIV	GCCGGC	1 cut(s)	364
NheI	GCTAGC	1 cut(s)	358
NlaIV	GGNNCC	4 cut(s)	513, 1494, 1540, 1759
NmeAIII	GCCGAG	3 cut(s)	337, 478, 2855
NmuCI	GTSAC	5 cut(s)	315, 1655, 1846, 2206, 2524
NruI	TCGCGA	1 cut(s)	186
NsiI	ATGCAT	3 cut(s)	960, 1206, 1254
NspI	RCATGY	4 cut(s)	1006, 1083, 1252, 2291
PauI	GCGCGC	1 cut(s)	1026
PciI	ACATGT	1 cut(s)	2287
PcsI	WCGNNNNNNNCGW	1 cut(s)	512
PdiI	GCCGGC	1 cut(s)	366
PdmI	GAANNNNTTC	4 cut(s)	798, 837, 1639, 2844
PfeI	GAWTC	5 cut(s)	349, 707, 1911, 2294, 2645
PinAI	ACCGGT	1 cut(s)	387
Ple19I	CGATCG	1 cut(s)	339
PleI	GAGTC	8 cut(s)	269, 292, 301, 322, 543, 989, 1160, 1920
PluTI	GGCGCC	1 cut(s)	1542
PpsI	GAGTC	8 cut(s)	269, 292, 301, 322, 543, 989, 1160, 1920
PscI	ACATGT	1 cut(s)	2287
PshAI	GACNNNNGTC	1 cut(s)	302
Psp1406I	AACGTT	1 cut(s)	2794
Psp6I	CCWGG	4 cut(s)	219, 867, 1629, 2327
PspGI	CCWGG	4 cut(s)	219, 867, 1629, 2327
PspN4I	GGNNCC	4 cut(s)	513, 1494, 1540, 1759
PspPI	GGNCC	6 cut(s)	10, 654, 1459, 1675, 1715, 2801
PstNI	CAGNNNCTG	1 cut(s)	603
PsuI	RGATCY	5 cut(s)	101, 511, 1295, 1706, 2479
PteI	GCGCGC	1 cut(s)	1026
PvuI	CGATCG	1 cut(s)	339
RruI	TCGCGA	1 cut(s)	186
RseI	CAYNNNNRTG	3 cut(s)	776, 1253, 2750
SalI	GTCGAC	1 cut(s)	295
SaqAI	TTAA	5 cut(s)	114, 1317, 1415, 1785, 1937
Sau96I	GGNCC	6 cut(s)	10, 654, 1459, 1675, 1715, 2801
ScaI	AGTACT	2 cut(s)	1314, 2418
SchI	GAGTC	8 cut(s)	269, 292, 302, 323, 543, 989, 1160, 1920
ScrFI	CCNGG	5 cut(s)	21, 221, 869, 1631, 2329
SfaNI	GCATC	3 cut(s)	1213, 1994, 2001
SfoI	GGCGCC	1 cut(s)	1540
SgrAI	CRCCGGYG	1 cut(s)	617
SinI	GGWCC	5 cut(s)	10, 654, 1459, 1715, 2801
SmiMI	CAYNNNNRTG	3 cut(s)	776, 1253, 2750
SmlI	CTYRAG	4 cut(s)	203, 965, 2219, 2435
SmoI	CTYRAG	4 cut(s)	203, 965, 2219, 2435
SspDI	GGCGCC	1 cut(s)	1538
SspI	AATATT	1 cut(s)	2759
StyD4I	CCNGG	5 cut(s)	19, 219, 867, 1629, 2327
StyI	CCWWGG	3 cut(s)	808, 1793, 2640
TaaI	ACNGT	7 cut(s)	587, 1280, 1719, 1783, 1826, 2317, 2734
TaiI	ACGT	6 cut(s)	1530, 1585, 1973, 2156, 2375, 2797
TaqI	TCGA	8 cut(s)	153, 296, 321, 335, 352, 381, 667, 758
TatI	WGTACW	6 cut(s)	1153, 1312, 1821, 1900, 2276, 2416
TauI	GCSGC	4 cut(s)	227, 311, 573, 2443
TfiI	GAWTC	5 cut(s)	349, 707, 1911, 2294, 2645
Tru1I	TTAA	5 cut(s)	114, 1317, 1415, 1785, 1937
Tru9I	TTAA	5 cut(s)	114, 1317, 1415, 1785, 1937
TscAI	CASTG	2 cut(s)	908, 1503
TseFI	GTSAC	5 cut(s)	315, 1655, 1846, 2206, 2524
TseI	GCWGC	7 cut(s)	536, 603, 659, 1076, 1301, 2244, 2664
Tsp45I	GTSAC	5 cut(s)	315, 1655, 1846, 2206, 2524
TspGWI	ACGGA	2 cut(s)	253, 504
TspRI	CASTG	2 cut(s)	908, 1503
VpaK11BI	GGWCC	5 cut(s)	10, 654, 1459, 1715, 2801
XapI	RAATTY	3 cut(s)	974, 1807, 2238
XbaI	TCTAGA	1 cut(s)	1828
XceI	RCATGY	4 cut(s)	1006, 1083, 1252, 2291
XmiI	GTMKAC	2 cut(s)	296, 2545
XmnI	GAANNNNTTC	4 cut(s)	798, 837, 1639, 2844
ZraI	GACGTC	2 cut(s)	1971, 2373
ZrmI	AGTACT	2 cut(s)	1314, 2418
Zsp2I	ATGCAT	3 cut(s)	960, 1206, 1254

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.