Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0004412

Physical Location & Seq

Genomic Coordinates Forward (+)

39665202 .. 39670371

Gene Structure 5170 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00418110.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2784 bp

ATGGAGGAGGACCACGCGCCCGGCGCGTGCAATCACTCTCTCAACCTGCCATCAATTTCCCCCACCGAACCCCTCTCCACCGAAACCCTAGCTTCCCTGAGATCCCTAATCATTAACCCCTCAACTCCCTCCCCCGCAATTTCTTCAATCATCGAAACCCTAACTCGCTCTCTCCAACTCAGTCGCGACCCGCTCCGCACTCTCAAGCTCCTCTCCGACCTGGCCGCCCGACACCCCCACCTCTCCGCCCTCGTCTTCCACTCCGTCCGCTCCGACTCGCTCCTCTCCACCGAGTCGACTCGCGTCGCCGCCGAGTCACTCGATGTCTTGGCTTCGATCGCAGAGCGGAATCGAGAGCTAGCGCCGGCTGTTGAGGAAATCGACGACCGGTTGTTGGCCTCGCTTTGTTTTAGTCCCTCTCCGGCGATACGGCCGTGGCTTCTCAGAAACGCCGAGAGGTTTGGTGTTCAGCCTTACTTGCTATCAAGCCTGTTTCTAGGGTTTACGAAGGATCCGTATCCGAATGTGAGGAAAGCTGCATTGGATGGACTCGTTGGTTTGAGTAAGAGCGGCGTTATTGACGACGGTGATATGATCAGAGGCTGCTACTTCCGCGCCGGTGAGCTTCTAAACGACATGGAAGACGGCGTTAGGTCCGCTGCAATTCGAGCGGTGTTTGCTTGGGGTCTAATCCTCATGGTGTGCGATTCGGAGGCGAAAGCATACTGGTCTGATGAAGTATTTGTCAAGATTTGTTCGATGGTAAGAGACATGAGCATGGAGGTCAGGATTGAAGCTTTTCATGCCCTTGGGAAGATAGGAATGGTATCTGAAGATATTCTTTTGCAAACCCTGTCAAAGAAAGTCCTGGTGGCTACTAAAGAAAAGAAATCTCTTGCTCAGTGTTCCGATGAACAACTGGAAGGATCAGGGTCTAGTGTTGCAGGAGCTTTTATGCATGGACTTGAGGATGAATTCCATGAGGTACGAAAAGCTGCATGTCATTCTTTGGGTACACTCACCATACTCTCTTCCAAGTTTGCTGGAGAAGCCTTGAACTTACTGATGGATGTACTGAATGATGACTCAATGCTTGTGCGGTTACAAGCTTTTGAAACTATGCATCAAATGGCAACTTTTGGTCATCTGAAGGTGCAAGAAAAACACATGCATATGTTTCTTGGCACTCTGGTTGACCGTAATACCCTGACAAGATCTGCTGCAAGGAAAGTACTTAAATTGGCAAAACTGCCAGATTTGAAGTTGTTTAGATCAACTATTGATACCCTTTTAGATAACTTGGAAATCTGTCCACAGGATGAAGCAGATATATTAAATGTTTTGTTTCATATTGGACGAAATCATGGAAAGTTTGTGGTCCGCATTATTGAGGAAGTTTCTCAGCAGATGGAGCCAGTGTCTAATGGCAAGTTAGGCTTTGATAACGTGAGGGTGGGCGCCTTGCTAGTTCTGGCCATCTCAGCTCCACTTTCACAAGAACGTGATTGCAGTATCCCACCTAGTATATTTTCTTATGCAGTTACATACCTGGGAAGGATTTCTCATGCTTTGAGTGACATTATTCATCAAGAGGGCCTGTTGAATTACTTGTCTCAATGCAGTAGATCCTCTGGACCGTATGATATGGAGTTTGACTTCAGCGCGAAAAGAGGTGGAACCTCTGAAATCTTGTCTCCAACAGTTAAGAAACCAAGGGAGGCTGGAAATTCTCTTGTAGAGTACAGTCTAGATGTGCAAGATGAAGTGACCAATTCAATGAATGTGGTTCTTGAAAAGGTGAAGGATATCTGGCCATTTGTACAAGCTGGATTCACGACTGAAGGACTCAGGACTTTAAGAAGTTGCAGGGAAGAATTGGCAACATTGACGTCTGGCTCTATTGCATCTGGTGGTATCTTTGCCTTTACGATGCAGTATATCAAGATCACGAAACTGCTTGCAAAGGCATGGGTGAACTTCGTGCCTTCAAGACACCTTCCTTCTAGCGGGATGGGAGACTTGGACTTTGTGTTGGGAAAGCTAGACGCGAGGCTTAGTGAACTGAAAAGTACGTTTATAGGATTGTCTAAAGTGGAAGAGTTGCATATTTTGGAGCTGGTACTTGTGACTTGTATGCTCAAGCTTTCTAAAGTTGAAATTTGCTGCCATCTTGGTACTTTGAGAAAGCTCTCTTGTACAATGGCACATGTGGAATCTCTCCTTGAAGATGGAACTGTTGAACCATCCAGGTTTATGATTGAAGTCAGAAAATTATTATCTGAAATTGGGACGTCTCTCAATGGTAGTTCCTGTAATCCAATTCTTTTTAGAAGAGTACTGGAAAGTTTCTTCCTTGAGCGGCTTGTGTTATGTGGTGGACTAAAGCACATAAAGGCAGATCTGGACATACCTGACAATGACTACGAACATCCTCTTTGCTTTGTCACAGGGTTGCCCGTTGGTATACCATGCCACATCACTCTTCATAATGTTTCGGTTGAAAGCAGATTATGGCTCAAAATGACTGCCAATGGAATTGATGATACTCAGTTTGTCTCCTTGGATTCAAACCTTTTTGGAGGCTGCGATGATCATAGAATAGTTGTGTTTACCGCACCATTTTACAAAACCCCGAAGGCATTTTCTTTTACAGTCAGCGTTTGCATTTGTATGGAATATTTGTCTGAGGATGAGGATGTGTCTTTTCTCAAACGTTGTGGACCTAGACATGAGCTTACATCTCTTTGCCGAGAAAAGGAAGTTTTTCTTTCTATGGTCAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006810 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009956 GO:0009987 GO:0010467 GO:0010496 GO:0012505 GO:0016070 GO:0016073 GO:0016180 GO:0022622 GO:0031123 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0034470 GO:0034472 GO:0034641 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043628 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048364 GO:0048731 GO:0048856 GO:0051179 GO:0051234 GO:0071704 GO:0090057 GO:0090304 GO:0097708 GO:0099402 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

927

Amino Acids

102.51

Weight (kDa)

5.75

Isoelectric Point (pI)

46.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
INTS4_C	PF25458	803 - 923	1.4e-09	Integrator subunit 4 family C-terminal Ig-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004412)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G08800 AT3G08800 AT3G08800
fragaria_vesca	FvH4_6g10600 FvH4_6g10600 FvH4_6g10600 FvH4_6g10600 FvH4_6g10600
malus_domestica	MD04G1166800.v1.1
prunus_persica	Prupe.6G285000_v2.0.a1 Prupe.6G285000_v2.0.a1
pyrus_communis	pycom01g05880 pycom04g14820
rosa_chinensis	RchiOBHm_Chr3g0461591 RchiOBHm_Chr3g0461601
rosa_laevigata	RLG00000024915
rosa_multiflora	Rmu_co8098260.1_g000001 Rmu_sc0003578.1_g000001 Rmu_sc0032177.1_g000001 Rmu_sc0040016.1_g000001 Rmu_ssc0000193.1_g000007
rosa_roxburghii	Rroxscaffold_6G00418110 Rroxscaffold_6G00418480
rosa_rugosa	Rorug03G0052200 Rorug03G0052200 Rorug03G0052300 Rorug03G0052300
rosa_samantha	Rh3AG110400 Rh3BG113600 Rh3CG115500 Rh3DG115300
rosa_wichuraiana	Rw3G009340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	1892, 2294
Acc36I	ACCTGC	1 cut(s)	54
AccB1I	GGYRCC	1 cut(s)	1457
AccBSI	CCGCTC	6 cut(s)	193, 270, 346, 570, 671, 2359
AccI	GTMKAC	2 cut(s)	296, 2464
AccII	CGCG	7 cut(s)	17, 26, 186, 303, 615, 1664, 2048
AclI	AACGTT	1 cut(s)	2713
AclWI	GGATC	5 cut(s)	96, 506, 519, 934, 1620
AcoI	YGGCCR	4 cut(s)	222, 431, 1473, 1811
AcsI	RAATTY	3 cut(s)	974, 1726, 2157
AcuI	CTGAAG	4 cut(s)	852, 1169, 1642, 1860
AcyI	GRCGYC	3 cut(s)	1458, 1889, 2291
AfiI	CCNNNNNNNGG	3 cut(s)	394, 2006, 2755
AflIII	ACRYGT	1 cut(s)	2206
AgeI	ACCGGT	1 cut(s)	387
AjnI	CCWGG	4 cut(s)	219, 867, 1548, 2246
AjuI	GAANNNNNNNTTGG	2 cut(s)	524, 556
Alw26I	GTCTC	6 cut(s)	762, 1617, 1698, 2010, 2298, 2560
AlwI	GGATC	5 cut(s)	96, 506, 519, 934, 1620
AlwNI	CAGNNNCTG	1 cut(s)	603
AoxI	GGCC	6 cut(s)	222, 396, 431, 1473, 1594, 1811
ApeKI	GCWGC	7 cut(s)	536, 603, 659, 995, 1220, 2163, 2583
ApoI	RAATTY	3 cut(s)	974, 1726, 2157
ArsI	GACNNNNNNTTYG	2 cut(s)	2009, 2041
AsiGI	ACCGGT	1 cut(s)	387
Asp700I	GAANNNNTTC	4 cut(s)	798, 837, 1558, 2763
AspLEI	GCGC	6 cut(s)	19, 26, 364, 617, 1460, 1664
AspS9I	GGNCC	6 cut(s)	10, 654, 1378, 1594, 1634, 2720
AsuC2I	CCSGG	1 cut(s)	21
AsuHPI	GGTGA	5 cut(s)	599, 632, 1012, 1810, 1984
AsuNHI	GCTAGC	1 cut(s)	358
AvaII	GGWCC	5 cut(s)	10, 654, 1378, 1634, 2720
BaeI	ACNNNNGTAYC	2 cut(s)	419, 452
BalI	TGGCCA	2 cut(s)	1475, 1813
BamHI	GGATCC	1 cut(s)	511
BanI	GGYRCC	1 cut(s)	1457
BbsI	GAAGAC	2 cut(s)	247, 648
BbvI	GCAGC	7 cut(s)	523, 590, 646, 982, 1207, 2150, 2570
BceAI	ACGGC	3 cut(s)	418, 446, 661
BciT130I	CCWGG	4 cut(s)	221, 869, 1550, 2248
BciVI	GTATCC	2 cut(s)	528, 1523
BclI	TGATCA	2 cut(s)	594, 2590
BcnI	CCSGG	1 cut(s)	21
BcoDI	GTCTC	6 cut(s)	762, 1617, 1698, 2010, 2298, 2560
BfoI	RGCGCY	2 cut(s)	365, 1461
BfuAI	ACCTGC	1 cut(s)	54
BfuI	GTATCC	2 cut(s)	528, 1523
BglII	AGATCT	2 cut(s)	1214, 2398
BmcAI	AGTACT	2 cut(s)	1233, 2337
Bme1390I	CCNGG	5 cut(s)	21, 221, 869, 1550, 2248
Bme18I	GGWCC	5 cut(s)	10, 654, 1378, 1634, 2720
BmgT120I	GGNCC	6 cut(s)	10, 654, 1378, 1594, 1634, 2720
BmiI	GGNNCC	4 cut(s)	513, 1413, 1459, 1678
BmrFI	CCNGG	5 cut(s)	21, 221, 869, 1550, 2248
BmsI	GCATC	3 cut(s)	1132, 1913, 1920
BmtI	GCTAGC	1 cut(s)	362
BoxI	GACNNNNGTC	1 cut(s)	302
BpiI	GAAGAC	2 cut(s)	247, 648
BpmI	CTGGAG	1 cut(s)	1065
BpuEI	CTTGAG	4 cut(s)	188, 986, 2123, 2375
BpuMI	CCSGG	1 cut(s)	21
BsaBI	GATNNNNATC	1 cut(s)	516
BsaHI	GRCGYC	3 cut(s)	1458, 1889, 2291
BsaJI	CCNNGG	5 cut(s)	434, 808, 1549, 1712, 2559
BsaWI	WCCGGW	1 cut(s)	387
BsaXI	ACNNNNNCTCC	2 cut(s)	1403, 1433
Bsc4I	CCNNNNNNNGG	3 cut(s)	394, 2006, 2755
Bse118I	RCCGGY	3 cut(s)	364, 387, 617
Bse1I	ACTGG	4 cut(s)	731, 924, 1415, 2343
Bse8I	GATNNNNATC	1 cut(s)	516
BseBI	CCWGG	4 cut(s)	221, 869, 1550, 2248
BseDI	CCNNGG	5 cut(s)	434, 808, 1549, 1712, 2559
BseGI	GGATG	9 cut(s)	550, 976, 1075, 1324, 2016, 2243, 2428, 2695, 2701
BseJI	GATNNNNATC	1 cut(s)	516
BseLI	CCNNNNNNNGG	3 cut(s)	394, 2006, 2755
BseMII	CTCAG	9 cut(s)	89, 193, 457, 914, 1415, 1494, 1861, 2561, 2676
BseNI	ACTGG	4 cut(s)	731, 924, 1415, 2343
BseRI	GAGGAG	3 cut(s)	20, 200, 272
BseX3I	CGGCCG	1 cut(s)	431
BseXI	GCAGC	7 cut(s)	523, 590, 646, 982, 1207, 2150, 2570
Bsh1236I	CGCG	7 cut(s)	17, 26, 186, 303, 615, 1664, 2048
Bsh1285I	CGRYCG	3 cut(s)	339, 388, 434
BshFI	GGCC	6 cut(s)	224, 398, 433, 1475, 1596, 1813
BshNI	GGYRCC	1 cut(s)	1457
BshTI	ACCGGT	1 cut(s)	387
BsiEI	CGRYCG	3 cut(s)	339, 388, 434
BsiSI	CCGG	5 cut(s)	21, 365, 388, 422, 618
BslFI	GGGAC	2 cut(s)	399, 2302
BslI	CCNNNNNNNGG	3 cut(s)	394, 2006, 2755
BsmAI	GTCTC	6 cut(s)	762, 1617, 1698, 2010, 2298, 2560
BsmBI	CGTCTC	1 cut(s)	2298
BsmFI	GGGAC	2 cut(s)	399, 2302
BsnI	GGCC	6 cut(s)	224, 398, 433, 1475, 1596, 1813
Bsp1407I	TGTACA	2 cut(s)	1819, 2195
Bsp68I	TCGCGA	1 cut(s)	186
BspANI	GGCC	6 cut(s)	224, 398, 433, 1475, 1596, 1813
BspCNI	CTCAG	9 cut(s)	90, 192, 456, 913, 1414, 1493, 1860, 2560, 2677
BspFNI	CGCG	7 cut(s)	17, 26, 186, 303, 615, 1664, 2048
BspLI	GGNNCC	4 cut(s)	513, 1413, 1459, 1678
BspMI	ACCTGC	1 cut(s)	54
BspOI	GCTAGC	1 cut(s)	362
BspPI	GGATC	5 cut(s)	96, 506, 519, 934, 1620
BspT107I	GGYRCC	1 cut(s)	1457
BsrBI	CCGCTC	6 cut(s)	193, 270, 346, 570, 671, 2359
BsrFI	RCCGGY	3 cut(s)	364, 387, 617
BsrGI	TGTACA	2 cut(s)	1819, 2195
BsrI	ACTGG	4 cut(s)	731, 924, 1415, 2343
BssAI	RCCGGY	3 cut(s)	364, 387, 617
BssECI	CCNNGG	5 cut(s)	434, 808, 1549, 1712, 2559
BssNAI	GTATAC	1 cut(s)	2465
BssNI	GRCGYC	3 cut(s)	1458, 1889, 2291
BssT1I	CCWWGG	3 cut(s)	808, 1712, 2559
Bst1107I	GTATAC	1 cut(s)	2465
Bst2UI	CCWGG	4 cut(s)	221, 869, 1550, 2248
Bst4CI	ACNGT	7 cut(s)	587, 1199, 1638, 1702, 1745, 2236, 2653
Bst6I	CTCTTC	4 cut(s)	1036, 2091, 2326, 2487
BstACI	GRCGYC	3 cut(s)	1458, 1889, 2291
BstAUI	TGTACA	2 cut(s)	1819, 2195
BstC8I	GCNNGC	4 cut(s)	28, 360, 366, 1959
BstDSI	CCRYGG	1 cut(s)	434
BstF5I	GGATG	9 cut(s)	550, 976, 1075, 1324, 2016, 2243, 2428, 2695, 2701
BstFNI	CGCG	7 cut(s)	17, 26, 186, 303, 615, 1664, 2048
BstH2I	RGCGCY	2 cut(s)	365, 1461
BstHHI	GCGC	6 cut(s)	19, 26, 364, 617, 1460, 1664
BstMAI	GTCTC	6 cut(s)	762, 1617, 1698, 2010, 2298, 2560
BstMCI	CGRYCG	3 cut(s)	339, 388, 434
BstNI	CCWGG	4 cut(s)	221, 869, 1550, 2248
BstNSI	RCATGY	3 cut(s)	1002, 1171, 2210
BstPAI	GACNNNNGTC	1 cut(s)	302
BstSCI	CCNGG	5 cut(s)	19, 219, 867, 1548, 2246
BstUI	CGCG	7 cut(s)	17, 26, 186, 303, 615, 1664, 2048
BstV1I	GCAGC	7 cut(s)	523, 590, 646, 982, 1207, 2150, 2570
BstV2I	GAAGAC	2 cut(s)	247, 648
BstX2I	RGATCY	5 cut(s)	101, 511, 1214, 1625, 2398
BstYI	RGATCY	5 cut(s)	101, 511, 1214, 1625, 2398
BstZ17I	GTATAC	1 cut(s)	2465
BstZI	CGGCCG	1 cut(s)	431
BsuI	GTATCC	2 cut(s)	528, 1523
BsuRI	GGCC	6 cut(s)	224, 398, 433, 1475, 1596, 1813
BtgI	CCRYGG	1 cut(s)	434
BtgZI	GCGATG	1 cut(s)	2601
BtsCI	GGATG	9 cut(s)	550, 976, 1075, 1324, 2016, 2243, 2428, 2695, 2701
BtsIMutI	CAGTG	2 cut(s)	908, 1422
BtuMI	TCGCGA	1 cut(s)	186
BveI	ACCTGC	1 cut(s)	54
Cac8I	GCNNGC	4 cut(s)	28, 360, 366, 1959
CaiI	CAGNNNCTG	1 cut(s)	603
CfoI	GCGC	6 cut(s)	19, 26, 364, 617, 1460, 1664
Cfr10I	RCCGGY	3 cut(s)	364, 387, 617
Cfr13I	GGNCC	6 cut(s)	10, 654, 1378, 1594, 1634, 2720
CseI	GACGC	2 cut(s)	292, 2054
CspAI	ACCGGT	1 cut(s)	387
CspCI	CAANNNNNGTGG	4 cut(s)	1764, 1799, 2698, 2733
DinI	GGCGCC	1 cut(s)	1459
EaeI	YGGCCR	4 cut(s)	222, 431, 1473, 1811
EagI	CGGCCG	1 cut(s)	431
Eam1104I	CTCTTC	4 cut(s)	1036, 2091, 2326, 2487
EarI	CTCTTC	4 cut(s)	1036, 2091, 2326, 2487
EciI	GGCGGA	1 cut(s)	235
EclXI	CGGCCG	1 cut(s)	431
Eco130I	CCWWGG	3 cut(s)	808, 1712, 2559
Eco32I	GATATC	1 cut(s)	1807
Eco47I	GGWCC	5 cut(s)	10, 654, 1378, 1634, 2720
Eco52I	CGGCCG	1 cut(s)	431
Eco57I	CTGAAG	4 cut(s)	852, 1169, 1642, 1860
EcoO109I	RGGNCCY	1 cut(s)	1594
EcoRI	GAATTC	1 cut(s)	974
EcoRII	CCWGG	4 cut(s)	219, 867, 1548, 2246
EcoRV	GATATC	1 cut(s)	1807
EcoT14I	CCWWGG	3 cut(s)	808, 1712, 2559
EcoT22I	ATGCAT	3 cut(s)	960, 1125, 1173
EgeI	GGCGCC	1 cut(s)	1459
EheI	GGCGCC	1 cut(s)	1459
ErhI	CCWWGG	3 cut(s)	808, 1712, 2559
Esp3I	CGTCTC	1 cut(s)	2298
FalI	AAGNNNNNCTT	6 cut(s)	825, 857, 1421, 1453, 2751, 2783
FaqI	GGGAC	2 cut(s)	399, 2302
FauI	CCCGC	3 cut(s)	142, 198, 2000
FauNDI	CATATG	1 cut(s)	1173
FbaI	TGATCA	2 cut(s)	594, 2590
FblI	GTMKAC	2 cut(s)	296, 2464
FokI	GGATG	9 cut(s)	557, 983, 1082, 1331, 2023, 2230, 2415, 2702, 2708
GlaI	GCGC	6 cut(s)	18, 25, 363, 616, 1459, 1663
GsuI	CTGGAG	1 cut(s)	1065
HaeII	RGCGCY	2 cut(s)	365, 1461
HaeIII	GGCC	6 cut(s)	224, 398, 433, 1475, 1596, 1813
HapII	CCGG	5 cut(s)	21, 365, 388, 422, 618
HgaI	GACGC	2 cut(s)	292, 2054
HhaI	GCGC	6 cut(s)	19, 26, 364, 617, 1460, 1664
Hin1I	GRCGYC	3 cut(s)	1458, 1889, 2291
Hin6I	GCGC	6 cut(s)	17, 24, 362, 615, 1458, 1662
HinP1I	GCGC	6 cut(s)	17, 24, 362, 615, 1458, 1662
HincII	GTYRAC	2 cut(s)	297, 1195
HindII	GTYRAC	2 cut(s)	297, 1195
HindIII	AAGCTT	3 cut(s)	795, 1107, 2141
HpaII	CCGG	5 cut(s)	21, 365, 388, 422, 618
HphI	GGTGA	5 cut(s)	599, 632, 1012, 1810, 1984
Hpy99I	CGWCG	3 cut(s)	308, 386, 587
HpyCH4III	ACNGT	7 cut(s)	587, 1199, 1638, 1702, 1745, 2236, 2653
HpyCH4IV	ACGT	6 cut(s)	1446, 1501, 1889, 2072, 2291, 2713
HpySE526I	ACGT	6 cut(s)	1446, 1501, 1889, 2072, 2291, 2713
Hsp92I	GRCGYC	3 cut(s)	1458, 1889, 2291
HspAI	GCGC	6 cut(s)	17, 24, 362, 615, 1458, 1662
KasI	GGCGCC	1 cut(s)	1457
KroI	GCCGGC	1 cut(s)	364
KroNI	GCCGGC	1 cut(s)	366
Ksp22I	TGATCA	2 cut(s)	594, 2590
LmnI	GCTCC	8 cut(s)	198, 213, 275, 285, 947, 1411, 1489, 2113
Lsp1109I	GCAGC	7 cut(s)	523, 590, 646, 982, 1207, 2150, 2570
LweI	GCATC	3 cut(s)	1132, 1913, 1920
MaeII	ACGT	6 cut(s)	1446, 1501, 1889, 2072, 2291, 2713
MaeIII	GTNAC	7 cut(s)	315, 1101, 1540, 1574, 1765, 2125, 2443
MbiI	CCGCTC	6 cut(s)	193, 270, 346, 570, 671, 2359
MflI	RGATCY	5 cut(s)	101, 511, 1214, 1625, 2398
MlsI	TGGCCA	2 cut(s)	1475, 1813
MluNI	TGGCCA	2 cut(s)	1475, 1813
Mly113I	GGCGCC	1 cut(s)	1458
MlyI	GAGTC	7 cut(s)	269, 292, 302, 323, 543, 1079, 1839
MmeI	TCCRAC	4 cut(s)	199, 240, 297, 1721
Mox20I	TGGCCA	2 cut(s)	1475, 1813
Mph1103I	ATGCAT	3 cut(s)	960, 1125, 1173
MroNI	GCCGGC	1 cut(s)	364
MroXI	GAANNNNTTC	4 cut(s)	798, 837, 1558, 2763
MscI	TGGCCA	2 cut(s)	1475, 1813
MseI	TTAA	5 cut(s)	114, 1236, 1334, 1704, 1856
MslI	CAYNNNNRTG	3 cut(s)	776, 1172, 2669
Msp20I	TGGCCA	2 cut(s)	1475, 1813
MspA1I	CMGCKG	1 cut(s)	659
MspI	CCGG	5 cut(s)	21, 365, 388, 422, 618
MspR9I	CCNGG	5 cut(s)	21, 221, 869, 1550, 2248
MvaI	CCWGG	4 cut(s)	221, 869, 1550, 2248
MvnI	CGCG	7 cut(s)	17, 26, 186, 303, 615, 1664, 2048
NaeI	GCCGGC	1 cut(s)	366
NarI	GGCGCC	1 cut(s)	1458
NciI	CCSGG	1 cut(s)	21
NdeI	CATATG	1 cut(s)	1173
NgoMIV	GCCGGC	1 cut(s)	364
NheI	GCTAGC	1 cut(s)	358
NlaIV	GGNNCC	4 cut(s)	513, 1413, 1459, 1678
NmeAIII	GCCGAG	3 cut(s)	337, 478, 2774
NmuCI	GTSAC	5 cut(s)	315, 1574, 1765, 2125, 2443
NruI	TCGCGA	1 cut(s)	186
NsiI	ATGCAT	3 cut(s)	960, 1125, 1173
NspI	RCATGY	3 cut(s)	1002, 1171, 2210
PciI	ACATGT	1 cut(s)	2206
PcsI	WCGNNNNNNNCGW	1 cut(s)	512
PdiI	GCCGGC	1 cut(s)	366
PdmI	GAANNNNTTC	4 cut(s)	798, 837, 1558, 2763
PfeI	GAWTC	5 cut(s)	349, 707, 1830, 2213, 2564
PinAI	ACCGGT	1 cut(s)	387
Ple19I	CGATCG	1 cut(s)	339
PleI	GAGTC	7 cut(s)	269, 292, 301, 322, 543, 1079, 1839
PluTI	GGCGCC	1 cut(s)	1461
PpsI	GAGTC	7 cut(s)	269, 292, 301, 322, 543, 1079, 1839
PscI	ACATGT	1 cut(s)	2206
PshAI	GACNNNNGTC	1 cut(s)	302
Psp1406I	AACGTT	1 cut(s)	2713
Psp6I	CCWGG	4 cut(s)	219, 867, 1548, 2246
PspGI	CCWGG	4 cut(s)	219, 867, 1548, 2246
PspN4I	GGNNCC	4 cut(s)	513, 1413, 1459, 1678
PspPI	GGNCC	6 cut(s)	10, 654, 1378, 1594, 1634, 2720
PstNI	CAGNNNCTG	1 cut(s)	603
PsuI	RGATCY	5 cut(s)	101, 511, 1214, 1625, 2398
PvuI	CGATCG	1 cut(s)	339
RruI	TCGCGA	1 cut(s)	186
RseI	CAYNNNNRTG	3 cut(s)	776, 1172, 2669
SalI	GTCGAC	1 cut(s)	295
SaqAI	TTAA	5 cut(s)	114, 1236, 1334, 1704, 1856
Sau96I	GGNCC	6 cut(s)	10, 654, 1378, 1594, 1634, 2720
ScaI	AGTACT	2 cut(s)	1233, 2337
SchI	GAGTC	7 cut(s)	269, 292, 302, 323, 543, 1079, 1839
ScrFI	CCNGG	5 cut(s)	21, 221, 869, 1550, 2248
SfaNI	GCATC	3 cut(s)	1132, 1913, 1920
SfoI	GGCGCC	1 cut(s)	1459
SgrAI	CRCCGGYG	1 cut(s)	617
SinI	GGWCC	5 cut(s)	10, 654, 1378, 1634, 2720
SmiMI	CAYNNNNRTG	3 cut(s)	776, 1172, 2669
SmlI	CTYRAG	4 cut(s)	203, 965, 2138, 2354
SmoI	CTYRAG	4 cut(s)	203, 965, 2138, 2354
SspDI	GGCGCC	1 cut(s)	1457
SspI	AATATT	1 cut(s)	2678
StyD4I	CCNGG	5 cut(s)	19, 219, 867, 1548, 2246
StyI	CCWWGG	3 cut(s)	808, 1712, 2559
TaaI	ACNGT	7 cut(s)	587, 1199, 1638, 1702, 1745, 2236, 2653
TaiI	ACGT	6 cut(s)	1449, 1504, 1892, 2075, 2294, 2716
TaqI	TCGA	8 cut(s)	153, 296, 321, 335, 352, 381, 667, 758
TatI	WGTACW	6 cut(s)	1072, 1231, 1740, 1819, 2195, 2335
TauI	GCSGC	4 cut(s)	227, 311, 573, 2362
TfiI	GAWTC	5 cut(s)	349, 707, 1830, 2213, 2564
Tru1I	TTAA	5 cut(s)	114, 1236, 1334, 1704, 1856
Tru9I	TTAA	5 cut(s)	114, 1236, 1334, 1704, 1856
TscAI	CASTG	2 cut(s)	908, 1422
TseFI	GTSAC	5 cut(s)	315, 1574, 1765, 2125, 2443
TseI	GCWGC	7 cut(s)	536, 603, 659, 995, 1220, 2163, 2583
Tsp45I	GTSAC	5 cut(s)	315, 1574, 1765, 2125, 2443
TspGWI	ACGGA	2 cut(s)	253, 504
TspRI	CASTG	2 cut(s)	908, 1422
VpaK11BI	GGWCC	5 cut(s)	10, 654, 1378, 1634, 2720
XapI	RAATTY	3 cut(s)	974, 1726, 2157
XbaI	TCTAGA	1 cut(s)	1747
XceI	RCATGY	3 cut(s)	1002, 1171, 2210
XmiI	GTMKAC	2 cut(s)	296, 2464
XmnI	GAANNNNTTC	4 cut(s)	798, 837, 1558, 2763
ZraI	GACGTC	2 cut(s)	1890, 2292
ZrmI	AGTACT	2 cut(s)	1233, 2337
Zsp2I	ATGCAT	3 cut(s)	960, 1125, 1173

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.