MD04G1174800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011413
Physical Location & Seq
Forward (+)
26609188 .. 26613903
4716 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1174800.v1.1.491

Sequence Viewer

Length: 1236 bp
ATGGATAGAATCGGTTTTCTGGCGGTGTTCTTCATCATCTTCGTCGTCGTTGAGGTTTCCGATGCTACTTATCTTCTCTCCAAGCACAGAGTTTTGATTGGTGAGCCTCAAAACGACACTACGGCAGTACCAGAGAACGTCAAGGTTAAGGAGGATTCTCCGGTCCCGAGTCCGCTGCTGCCTTCTACGGTGTCAAGATCTGGTGGAAATGGGTCGGATACTAAGCCGGTTGATGATCCGAGTAAGGACAAAGTGGAGACTCCGCAACCCACGAAGACTACGAAACAGATAGTGGATCAGGTCCCCAAGGGGGGCTTGAATAATCAAACTAAAAAGGGTGAAGGGAAGGATCCGGAAACAGAAAAGAAGGATCCGGAGCCTGACAAGACATTGAAGAAGGATCCGGAAACAGAAAAGAAGAAGCCGGACGCTGACAAGGTGGATCCTCAGCTGGTTCATGACAAGTCAAAGGATGATAATGTGACTCGTTCCGTGGATGAAGGTGGTAAGAAAAAGAGTAAGGAGAAGGAGAAGGAGAAGAAGAAGAATGATGATGTGACCCAATTGCCAAAAGAGGATAAGGAAAGCTGTGATGGGGTAATCAAAACATGTGAGGTCAAGGGTGTGGCGATTGCTTGCATTAAGAGCTTCGATAGTGGGTCCAAAGAAGTGGCCATTCTGGTCCAAAACGTAGGAGACAGCACTTTGAAGGCAAAGCTTTCTGCAGATGATGCCAACAAGGATCTAGAGATCCTCAAACACAAAAATAAAAAGGTCAATATATCAGTTGATATGGGCAAAAGCACCACAATAACACTAAATTCTGGAAATGGAGAATGTGAGCTTCTTATGGATCCTCTCCCTCTGGCACCTGAAGGAAACTTATTCATGCGTTTCCCTTCGCTCGAGACTTTAGCGACCCCGATCAATGCTGCGTACTTCTTGATTATTACGGTGCTAATTTTTGGAGGAACGTGGGCTTGCTGCTTGGTCAGGAAGAGGAAACAACGTACTGGAGGTGGAGTCCCATATCAGGAACTTGAAATGGGATTGCCAGAATCTGTTTCAGCAACTGCTGTGGAAACAGCTGAAGGTTGGGACGAAGGTTGGGATGATGATTGGGATGGCGATAATGCGGTGAAAACACCTGGGACGCATCTGGGAAGCATCTCTGCGAATGGCCTTACATCGAGGACCGCGAACAAGGATGGGTGGGATAATGATTGGGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

412

Amino Acids

44.76

Weight (kDa)

5.08

Isoelectric Point (pI)

33.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7356 PF24053 191 - 286 5.2e-27 Domain of unknown function (DUF7356)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011413)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51580 AT3G51580
fragaria_vesca FvH4_6g09850
malus_domestica MD04G1174800.v1.1 MD12G1188500.v1.1
prunus_persica Prupe.6G293800_v2.0.a1
pyrus_communis pycom04g15420 pycom12g17610
rosa_chinensis RchiOBHm_Chr3g0460451
rosa_laevigata RLG00000025010
rosa_multiflora Rmu_sc0004267.1_g000009 Rmu_sc0021028.1_g000003
rosa_roxburghii Rroxscaffold_6G00419470
rosa_rugosa Rorug03G0042600 Rorug03G0042700
rosa_samantha Rh3AG100800 Rh3BG104800 Rh3CG106600 Rh3DG106100
rosa_wichuraiana Rw3G008580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 868
AccII CGCG 1 cut(s) 1199
AccIII TCCGGA 3 cut(s) 352, 373, 403
AciI CCGC 5 cut(s) 23, 173, 263, 1136, 1197
AcoI YGGCCR 1 cut(s) 672
AcsI RAATTY 1 cut(s) 820
AcuI CTGAAG 2 cut(s) 894, 1110
AfaI GTAC 3 cut(s) 129, 938, 1012
AfiI CCNNNNNNNGG 3 cut(s) 310, 311, 1033
AflIII ACRYGT 1 cut(s) 608
AgsI TTSAA 4 cut(s) 319, 394, 709, 1043
AjnI CCWGG 1 cut(s) 1147
AluBI AGCT 6 cut(s) 451, 588, 648, 718, 844, 1088
AluI AGCT 6 cut(s) 451, 588, 648, 718, 844, 1088
Alw26I GTCTC 3 cut(s) 251, 690, 902
AlwNI CAGNNNCTG 2 cut(s) 1061, 1073
Ama87I CYCGRG 2 cut(s) 166, 905
Aor13HI TCCGGA 3 cut(s) 352, 373, 403
AoxI GGCC 2 cut(s) 672, 1180
ApeKI GCWGC 4 cut(s) 175, 178, 932, 984
ApoI RAATTY 1 cut(s) 820
AspS9I GGNCC 5 cut(s) 163, 301, 660, 682, 1194
AsuHPI GGTGA 3 cut(s) 113, 350, 1150
AvaI CYCGRG 2 cut(s) 166, 905
AvaII GGWCC 5 cut(s) 163, 301, 660, 682, 1194
BaeI ACNNNNGTAYC 2 cut(s) 111, 144
BalI TGGCCA 1 cut(s) 674
BamHI GGATCC 5 cut(s) 349, 370, 400, 442, 853
BanI GGYRCC 1 cut(s) 868
BbsI GAAGAC 1 cut(s) 281
BbvCI CCTCAGC 1 cut(s) 447
BbvI GCAGC 4 cut(s) 162, 165, 919, 971
BccI CCATC 3 cut(s) 587, 1118, 1202
BceAI ACGGC 1 cut(s) 138
BcgI CGANNNNNNTGC 2 cut(s) 157, 191
BciT130I CCWGG 1 cut(s) 1149
BciVI GTATCC 1 cut(s) 211
BcoDI GTCTC 3 cut(s) 251, 690, 902
BfaI CTAG 1 cut(s) 746
BfmI CTRYAG 1 cut(s) 723
BfuI GTATCC 1 cut(s) 211
BglII AGATCT 1 cut(s) 197
BisI GCNGC 4 cut(s) 176, 179, 933, 985
BlsI GCNGC 4 cut(s) 177, 180, 934, 986
Bme1390I CCNGG 1 cut(s) 1149
Bme18I GGWCC 5 cut(s) 163, 301, 660, 682, 1194
BmeT110I CYCGRG 2 cut(s) 166, 905
BmgT120I GGNCC 5 cut(s) 163, 301, 660, 682, 1194
BmrFI CCNGG 1 cut(s) 1149
BmsI GCATC 4 cut(s) 52, 721, 1165, 1176
BpiI GAAGAC 1 cut(s) 281
BpmI CTGGAG 1 cut(s) 1035
Bpu10I CCTNAGC 1 cut(s) 447
BsaJI CCNNGG 3 cut(s) 306, 492, 1148
BsaWI WCCGGW 4 cut(s) 160, 352, 373, 403
BsaXI ACNNNNNCTCC 2 cut(s) 1008, 1038
Bsc4I CCNNNNNNNGG 3 cut(s) 310, 311, 1033
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 1 cut(s) 1018
BseAI TCCGGA 3 cut(s) 352, 373, 403
BseBI CCWGG 1 cut(s) 1149
BseDI CCNNGG 3 cut(s) 306, 492, 1148
BseGI GGATG 6 cut(s) 478, 502, 1117, 1129, 1213, 1234
BseLI CCNNNNNNNGG 3 cut(s) 310, 311, 1033
BseMII CTCAG 1 cut(s) 461
BseNI ACTGG 1 cut(s) 1018
BseXI GCAGC 4 cut(s) 162, 165, 919, 971
Bsh1236I CGCG 1 cut(s) 1199
BshFI GGCC 2 cut(s) 674, 1182
BshNI GGYRCC 1 cut(s) 868
BsiHKCI CYCGRG 2 cut(s) 166, 905
BsiSI CCGG 6 cut(s) 161, 227, 353, 374, 404, 425
BslFI GGGAC 5 cut(s) 149, 287, 1010, 1112, 1165
BslI CCNNNNNNNGG 3 cut(s) 310, 311, 1033
BsmAI GTCTC 3 cut(s) 251, 690, 902
BsmFI GGGAC 5 cut(s) 149, 287, 1010, 1112, 1165
BsnI GGCC 2 cut(s) 674, 1182
BsoBI CYCGRG 2 cut(s) 166, 905
Bsp13I TCCGGA 3 cut(s) 352, 373, 403
BspACI CCGC 5 cut(s) 23, 173, 263, 1136, 1197
BspANI GGCC 2 cut(s) 674, 1182
BspCNI CTCAG 1 cut(s) 460
BspEI TCCGGA 3 cut(s) 352, 373, 403
BspFNI CGCG 1 cut(s) 1199
BspHI TCATGA 1 cut(s) 457
BspMAI CTGCAG 1 cut(s) 727
BspT107I GGYRCC 1 cut(s) 868
BsrFI RCCGGY 1 cut(s) 226
BsrI ACTGG 1 cut(s) 1018
BssAI RCCGGY 1 cut(s) 226
BssECI CCNNGG 3 cut(s) 306, 492, 1148
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 1149
Bst4CI ACNGT 2 cut(s) 190, 955
Bst6I CTCTTC 1 cut(s) 992
BstAPI GCANNNNNTGC 1 cut(s) 731
BstC8I GCNNGC 2 cut(s) 637, 982
BstDEI CTNAG 2 cut(s) 222, 447
BstDSI CCRYGG 1 cut(s) 492
BstF5I GGATG 6 cut(s) 478, 502, 1117, 1129, 1213, 1234
BstFNI CGCG 1 cut(s) 1199
BstMAI GTCTC 3 cut(s) 251, 690, 902
BstMWI GCNNNNNNNGC 2 cut(s) 645, 731
BstNI CCWGG 1 cut(s) 1149
BstNSI RCATGY 1 cut(s) 612
BstSCI CCNGG 1 cut(s) 1147
BstSFI CTRYAG 1 cut(s) 723
BstUI CGCG 1 cut(s) 1199
BstV1I GCAGC 4 cut(s) 162, 165, 919, 971
BstV2I GAAGAC 1 cut(s) 281
BstX2I RGATCY 8 cut(s) 197, 349, 370, 400, 442, 742, 750, 853
BstXI CCANNNNNNTGG 1 cut(s) 670
BstYI RGATCY 8 cut(s) 197, 349, 370, 400, 442, 742, 750, 853
BsuI GTATCC 1 cut(s) 211
BsuRI GGCC 2 cut(s) 674, 1182
BtgI CCRYGG 1 cut(s) 492
BtsCI GGATG 6 cut(s) 478, 502, 1117, 1129, 1213, 1234
Cac8I GCNNGC 2 cut(s) 637, 982
CaiI CAGNNNCTG 2 cut(s) 1061, 1073
CciI TCATGA 1 cut(s) 457
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 5 cut(s) 163, 301, 660, 682, 1194
CseI GACGC 2 cut(s) 437, 1162
Csp6I GTAC 3 cut(s) 128, 937, 1011
CspCI CAANNNNNGTGG 2 cut(s) 1059, 1094
CviAII CATG 3 cut(s) 458, 609, 889
CviQI GTAC 3 cut(s) 128, 937, 1011
DdeI CTNAG 2 cut(s) 222, 447
EaeI YGGCCR 1 cut(s) 672
Eam1104I CTCTTC 1 cut(s) 992
EarI CTCTTC 1 cut(s) 992
Eco130I CCWWGG 1 cut(s) 306
Eco47I GGWCC 5 cut(s) 163, 301, 660, 682, 1194
Eco57I CTGAAG 2 cut(s) 894, 1110
Eco88I CYCGRG 2 cut(s) 166, 905
EcoO109I RGGNCCY 1 cut(s) 301
EcoRII CCWGG 1 cut(s) 1147
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 3 cut(s) 461, 612, 892
FaiI YATR 7 cut(s) 459, 610, 782, 794, 851, 890, 1030
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FaqI GGGAC 5 cut(s) 149, 287, 1010, 1112, 1165
FatI CATG 3 cut(s) 457, 608, 888
Fnu4HI GCNGC 4 cut(s) 176, 179, 933, 985
FokI GGATG 5 cut(s) 485, 509, 1124, 1136, 1220
Fsp4HI GCNGC 4 cut(s) 176, 179, 933, 985
FspBI CTAG 1 cut(s) 746
GluI GCNGC 4 cut(s) 176, 179, 933, 985
GsuI CTGGAG 1 cut(s) 1035
HaeIII GGCC 2 cut(s) 674, 1182
HapII CCGG 6 cut(s) 161, 227, 353, 374, 404, 425
HgaI GACGC 2 cut(s) 437, 1162
Hin1II CATG 3 cut(s) 461, 612, 892
HindIII AAGCTT 1 cut(s) 716
HinfI GANTC 7 cut(s) 9, 155, 169, 259, 484, 1023, 1058
HpaII CCGG 6 cut(s) 161, 227, 353, 374, 404, 425
HphI GGTGA 3 cut(s) 113, 350, 1150
Hpy188I TCNGA 3 cut(s) 61, 217, 240
Hpy99I CGWCG 2 cut(s) 47, 50
HpyCH4III ACNGT 2 cut(s) 190, 955
HpyCH4IV ACGT 4 cut(s) 138, 690, 974, 1009
HpyCH4V TGCA 2 cut(s) 639, 725
HpyF10VI GCNNNNNNNGC 2 cut(s) 645, 731
HpyF3I CTNAG 2 cut(s) 222, 447
HpySE526I ACGT 4 cut(s) 138, 690, 974, 1009
Hsp92II CATG 3 cut(s) 461, 612, 892
Kpn2I TCCGGA 3 cut(s) 352, 373, 403
LmnI GCTCC 1 cut(s) 376
Lsp1109I GCAGC 4 cut(s) 162, 165, 919, 971
LweI GCATC 4 cut(s) 52, 721, 1165, 1176
MaeI CTAG 1 cut(s) 746
MaeII ACGT 4 cut(s) 138, 690, 974, 1009
MaeIII GTNAC 2 cut(s) 481, 556
MfeI CAATTG 1 cut(s) 563
MflI RGATCY 8 cut(s) 197, 349, 370, 400, 442, 742, 750, 853
MlsI TGGCCA 1 cut(s) 674
MluCI AATT 3 cut(s) 563, 820, 960
MluNI TGGCCA 1 cut(s) 674
MlyI GAGTC 4 cut(s) 178, 253, 478, 1032
MmeI TCCRAC 1 cut(s) 195
Mox20I TGGCCA 1 cut(s) 674
MroI TCCGGA 3 cut(s) 352, 373, 403
MscI TGGCCA 1 cut(s) 674
MseI TTAA 2 cut(s) 147, 642
Msp20I TGGCCA 1 cut(s) 674
MspA1I CMGCKG 3 cut(s) 175, 451, 1088
MspI CCGG 6 cut(s) 161, 227, 353, 374, 404, 425
MspR9I CCNGG 1 cut(s) 1149
MunI CAATTG 1 cut(s) 563
MvaI CCWGG 1 cut(s) 1149
MvnI CGCG 1 cut(s) 1199
MwoI GCNNNNNNNGC 2 cut(s) 645, 731
NlaIII CATG 3 cut(s) 461, 612, 892
NmuCI GTSAC 2 cut(s) 481, 556
NspI RCATGY 1 cut(s) 612
PaeR7I CTCGAG 1 cut(s) 905
PagI TCATGA 1 cut(s) 457
PciI ACATGT 1 cut(s) 608
PcsI WCGNNNNNNNCGW 2 cut(s) 278, 1196
PfeI GAWTC 3 cut(s) 9, 155, 1058
PkrI GCNGC 4 cut(s) 177, 180, 934, 986
PleI GAGTC 4 cut(s) 177, 253, 478, 1031
PpsI GAGTC 4 cut(s) 177, 253, 478, 1031
PpuMI RGGWCCY 1 cut(s) 301
PscI ACATGT 1 cut(s) 608
Psp5II RGGWCCY 1 cut(s) 301
Psp6I CCWGG 1 cut(s) 1147
PspGI CCWGG 1 cut(s) 1147
PspPI GGNCC 5 cut(s) 163, 301, 660, 682, 1194
PspPPI RGGWCCY 1 cut(s) 301
PstI CTGCAG 1 cut(s) 727
PstNI CAGNNNCTG 2 cut(s) 1061, 1073
PsuI RGATCY 8 cut(s) 197, 349, 370, 400, 442, 742, 750, 853
PvuII CAGCTG 2 cut(s) 451, 1088
RsaI GTAC 3 cut(s) 129, 938, 1012
RsaNI GTAC 3 cut(s) 128, 937, 1011
SaqAI TTAA 2 cut(s) 147, 642
SatI GCNGC 4 cut(s) 176, 179, 933, 985
Sau96I GGNCC 5 cut(s) 163, 301, 660, 682, 1194
SchI GAGTC 4 cut(s) 178, 253, 478, 1032
ScrFI CCNGG 1 cut(s) 1149
SfaNI GCATC 4 cut(s) 52, 721, 1165, 1176
SfcI CTRYAG 1 cut(s) 723
Sfr274I CTCGAG 1 cut(s) 905
SinI GGWCC 5 cut(s) 163, 301, 660, 682, 1194
SlaI CTCGAG 1 cut(s) 905
SmlI CTYRAG 1 cut(s) 905
SmoI CTYRAG 1 cut(s) 905
Sse9I AATT 3 cut(s) 563, 820, 960
SsiI CCGC 5 cut(s) 23, 173, 263, 1136, 1197
SspMI CTAG 1 cut(s) 746
StyD4I CCNGG 1 cut(s) 1147
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 2 cut(s) 190, 955
TaiI ACGT 4 cut(s) 141, 693, 977, 1012
TaqI TCGA 3 cut(s) 651, 906, 1190
TasI AATT 3 cut(s) 563, 820, 960
TfiI GAWTC 3 cut(s) 9, 155, 1058
Tru1I TTAA 2 cut(s) 147, 642
Tru9I TTAA 2 cut(s) 147, 642
TseFI GTSAC 2 cut(s) 481, 556
TseI GCWGC 4 cut(s) 175, 178, 932, 984
Tsp45I GTSAC 2 cut(s) 481, 556
TspDTI ATGAA 4 cut(s) 22, 446, 513, 877
TspGWI ACGGA 1 cut(s) 481
VpaK11BI GGWCC 5 cut(s) 163, 301, 660, 682, 1194
XapI RAATTY 1 cut(s) 820
XbaI TCTAGA 1 cut(s) 745
XceI RCATGY 1 cut(s) 612
XhoI CTCGAG 1 cut(s) 905
XspI CTAG 1 cut(s) 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.