RLG00000025010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0011413
Physical Location & Seq
Forward (+)
41291175 .. 41294144
2970 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025010

Sequence Viewer

Length: 1266 bp
ATGGACCGAACTGGTTTTCTGGCGGTGTTCTTATTGCTCTTCATCGTCTCCGATGTCGCCAATGCTTCGATGCTCACCAAGCATAGATTTTTGATCGGTGATCCTCTGAAGGGCAATTCGGAAAATCCCACATTACCGGGTAAGGATTCTCCGGTGGCGAATCCACCTCCGCCTTCTAAACCTGATCCTAACCCTAATCCTAAGCCAGTTGATGATGGGAAGAACAAAACGGGCTCTGCCCCACCCGCCAAGACTCCGAAGACGCCAGAACCGAATCCGGAGCCGAAAGGTTCAAATAACAGTACTAAACCGGCTCCGGTAGGTCCACCAACTGATACCGGTGGTGGGAAGAATAACCAAACACAGAAGAAGGATCCAAATCCTGAGCAGAAGCCGAAGGAGAAACCAACTCCAGAGCCTGAACAGAAGCCGAAGGAGAATCCAACTCCGGAGCCTGAGCAAAAGCCGAAGGAGAATCCAACTCCGGAGCCTGAGCAGAAGCCGAAGGAGAATCCAACTCCGGTGCCTGGCAAGACACCGGAAGAGGATCCAAGTGCAAAGACTCCAGAAATAGAGAAGACTAAGAATAAAAGTGACAATGTGACTAATACACCGAATGCGAAAAAAGAAAGCTGCGATGGGGTAATCAAAACGTGCAGTAATACGGATATGGTTGCCTGCATTAAGAGCTTCAATAGGGAATCAACAGAAGCAGTCATTCTGGTCCAAAATGTACGCGACAGCACATGGAACGGAAGCCTTTCTGCAGAAGATACCAGGGACCTGGTGATGCACAAACACGAATATAAAAAGATCAAGCTCAATATGGGTAAAAGCAACAAAATAGTACTAAAAGCTGAAAATGGAGAATGCGTGCTTGAGATGGACCCTTTTGTATCTGAAGGAAACTTTTTCATGCGTTTCCCTTCTTATGAGAAGTTAGTGACGCCAATCAATGGTGCATACTTCTTGATTGTTACAGTGCTAATTTTTGGAGGGATGTGGGTTTGCTGCCTGGTTAGGAGGAGGAAACAGCGCACTGGAGGTGGAGTTCCTTATCAGGAACTTGAAATGGCATTGCCGGAATCGGCTTCAGCAACTGATGTGGTTACGGCAGAAGGTTGGGATCAGGGTTGGGATGACGATTGGGATGGAGATAATGCAGTGAAGTCACCTGGAGGGCATCTGGTGGGAAGCATATCTGCTAATGGTCTTACTGCAAGATCTTTGAACAAAGATGGGTGGGAAAATAACTGGGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

422

Amino Acids

45.95

Weight (kDa)

5.58

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7356 PF24053 206 - 297 9.7e-19 Domain of unknown function (DUF7356)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011413)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51580 AT3G51580
fragaria_vesca FvH4_6g09850
malus_domestica MD04G1174800.v1.1 MD12G1188500.v1.1
prunus_persica Prupe.6G293800_v2.0.a1
pyrus_communis pycom04g15420 pycom12g17610
rosa_chinensis RchiOBHm_Chr3g0460451
rosa_laevigata RLG00000025010
rosa_multiflora Rmu_sc0004267.1_g000009 Rmu_sc0021028.1_g000003
rosa_roxburghii Rroxscaffold_6G00419470
rosa_rugosa Rorug03G0042600 Rorug03G0042700
rosa_samantha Rh3AG100800 Rh3BG104800 Rh3CG106600 Rh3DG106100
rosa_wichuraiana Rw3G008580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 523
AccB7I CCANNNNNTGG 1 cut(s) 956
AccII CGCG 1 cut(s) 738
AccIII TCCGGA 3 cut(s) 277, 448, 484
AciI CCGC 3 cut(s) 23, 170, 246
AclWI GGATC 7 cut(s) 95, 179, 368, 381, 542, 555, 1134
AcuI CTGAAG 3 cut(s) 128, 921, 1077
AcyI GRCGYC 2 cut(s) 263, 947
AfaI GTAC 3 cut(s) 304, 735, 849
AfiI CCNNNNNNNGG 4 cut(s) 110, 345, 527, 956
AgeI ACCGGT 1 cut(s) 338
AgsI TTSAA 4 cut(s) 294, 694, 1070, 1231
AjnI CCWGG 5 cut(s) 526, 776, 783, 1014, 1174
AloI GAACNNNNNNTCC 2 cut(s) 1035, 1067
AluBI AGCT 4 cut(s) 633, 690, 820, 857
AluI AGCT 4 cut(s) 633, 690, 820, 857
Alw26I GTCTC 1 cut(s) 52
AlwI GGATC 7 cut(s) 95, 179, 368, 381, 542, 555, 1134
AlwNI CAGNNNCTG 2 cut(s) 419, 1100
Aor13HI TCCGGA 3 cut(s) 277, 448, 484
ApeKI GCWGC 2 cut(s) 633, 1011
AsiGI ACCGGT 1 cut(s) 338
Asp700I GAANNNNTTC 1 cut(s) 760
AspLEI GCGC 1 cut(s) 1038
AspS9I GGNCC 5 cut(s) 4, 323, 724, 781, 886
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 4 cut(s) 67, 110, 799, 1164
AvaII GGWCC 5 cut(s) 4, 323, 724, 781, 886
BamHI GGATCC 2 cut(s) 373, 547
BanI GGYRCC 1 cut(s) 523
BanII GRGCYC 1 cut(s) 236
BbsI GAAGAC 2 cut(s) 266, 584
BbvI GCAGC 2 cut(s) 620, 998
BccI CCATC 5 cut(s) 209, 632, 877, 1145, 1232
BceAI ACGGC 1 cut(s) 1128
BciT130I CCWGG 5 cut(s) 528, 778, 785, 1016, 1176
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 1 cut(s) 52
BfmI CTRYAG 1 cut(s) 765
BglII AGATCT 1 cut(s) 1223
BisI GCNGC 2 cut(s) 634, 1012
BlsI GCNGC 2 cut(s) 635, 1013
BmcAI AGTACT 2 cut(s) 304, 849
Bme1390I CCNGG 6 cut(s) 138, 528, 778, 785, 1016, 1176
Bme18I GGWCC 5 cut(s) 4, 323, 724, 781, 886
BmgT120I GGNCC 5 cut(s) 4, 323, 724, 781, 886
BmiI GGNNCC 9 cut(s) 282, 315, 375, 453, 489, 525, 549, 782, 888
BmrFI CCNGG 6 cut(s) 138, 528, 778, 785, 1016, 1176
BmrI ACTGGG 1 cut(s) 1264
BmsI GCATC 3 cut(s) 60, 780, 1192
BmuI ACTGGG 1 cut(s) 1264
BpiI GAAGAC 2 cut(s) 266, 584
BpmI CTGGAG 4 cut(s) 396, 549, 1062, 1197
Bpu10I CCTNAGC 4 cut(s) 201, 384, 456, 492
BpuEI CTTGAG 1 cut(s) 899
BpuMI CCSGG 1 cut(s) 138
BsaBI GATNNNNATC 1 cut(s) 378
BsaHI GRCGYC 2 cut(s) 263, 947
BsaJI CCNNGG 1 cut(s) 777
BsaWI WCCGGW 8 cut(s) 151, 277, 316, 338, 448, 484, 520, 538
BsaXI ACNNNNNCTCC 4 cut(s) 858, 888, 1035, 1065
Bsc4I CCNNNNNNNGG 4 cut(s) 110, 345, 527, 956
Bse118I RCCGGY 2 cut(s) 310, 338
Bse1I ACTGG 4 cut(s) 16, 206, 1045, 1259
Bse3DI GCAATG 1 cut(s) 1076
Bse8I GATNNNNATC 1 cut(s) 378
BseAI TCCGGA 3 cut(s) 277, 448, 484
BseBI CCWGG 5 cut(s) 528, 778, 785, 1016, 1176
BseDI CCNNGG 1 cut(s) 777
BseGI GGATG 4 cut(s) 1005, 1144, 1156, 1264
BseJI GATNNNNATC 1 cut(s) 378
BseLI CCNNNNNNNGG 4 cut(s) 110, 345, 527, 956
BseMI GCAATG 1 cut(s) 1076
BseMII CTCAG 3 cut(s) 375, 447, 483
BseNI ACTGG 4 cut(s) 16, 206, 1045, 1259
BseRI GAGGAG 1 cut(s) 1039
BseXI GCAGC 2 cut(s) 620, 998
BsgI GTGCAG 1 cut(s) 676
Bsh1236I CGCG 1 cut(s) 738
BshNI GGYRCC 1 cut(s) 523
BshTI ACCGGT 1 cut(s) 338
BslFI GGGAC 1 cut(s) 794
BslI CCNNNNNNNGG 4 cut(s) 110, 345, 527, 956
BsmAI GTCTC 1 cut(s) 52
BsmBI CGTCTC 1 cut(s) 52
BsmFI GGGAC 1 cut(s) 794
BsmI GAATGC 2 cut(s) 622, 875
Bsp1286I GDGCHC 1 cut(s) 236
Bsp13I TCCGGA 3 cut(s) 277, 448, 484
Bsp143I GATC 8 cut(s) 93, 100, 184, 373, 547, 813, 1126, 1223
BspACI CCGC 3 cut(s) 23, 170, 246
BspCNI CTCAG 3 cut(s) 376, 448, 484
BspEI TCCGGA 3 cut(s) 277, 448, 484
BspFNI CGCG 1 cut(s) 738
BspLI GGNNCC 9 cut(s) 282, 315, 375, 453, 489, 525, 549, 782, 888
BspMAI CTGCAG 1 cut(s) 769
BspPI GGATC 7 cut(s) 95, 179, 368, 381, 542, 555, 1134
BspQI GCTCTTC 1 cut(s) 44
BspT107I GGYRCC 1 cut(s) 523
BsrDI GCAATG 1 cut(s) 1076
BsrFI RCCGGY 2 cut(s) 310, 338
BsrI ACTGG 4 cut(s) 16, 206, 1045, 1259
BssAI RCCGGY 2 cut(s) 310, 338
BssECI CCNNGG 1 cut(s) 777
BssMI GATC 8 cut(s) 93, 100, 184, 373, 547, 813, 1126, 1223
BssNI GRCGYC 2 cut(s) 263, 947
Bst2UI CCWGG 5 cut(s) 528, 778, 785, 1016, 1176
Bst4CI ACNGT 2 cut(s) 302, 982
Bst6I CTCTTC 2 cut(s) 44, 537
BstACI GRCGYC 2 cut(s) 263, 947
BstC8I GCNNGC 2 cut(s) 679, 875
BstDEI CTNAG 5 cut(s) 201, 384, 456, 492, 582
BstF5I GGATG 4 cut(s) 1005, 1144, 1156, 1264
BstFNI CGCG 1 cut(s) 738
BstHHI GCGC 1 cut(s) 1038
BstKTI GATC 8 cut(s) 96, 103, 187, 376, 550, 816, 1129, 1226
BstMAI GTCTC 1 cut(s) 52
BstMBI GATC 8 cut(s) 93, 100, 184, 373, 547, 813, 1126, 1223
BstMWI GCNNNNNNNGC 3 cut(s) 79, 245, 687
BstNI CCWGG 5 cut(s) 528, 778, 785, 1016, 1176
BstSCI CCNGG 6 cut(s) 136, 526, 776, 783, 1014, 1174
BstSFI CTRYAG 1 cut(s) 765
BstUI CGCG 1 cut(s) 738
BstV1I GCAGC 2 cut(s) 620, 998
BstV2I GAAGAC 2 cut(s) 266, 584
BstX2I RGATCY 3 cut(s) 373, 547, 1223
BstXI CCANNNNNNTGG 1 cut(s) 784
BstYI RGATCY 3 cut(s) 373, 547, 1223
BtgZI GCGATG 1 cut(s) 651
BtsCI GGATG 4 cut(s) 1005, 1144, 1156, 1264
BtsI GCAGTG 1 cut(s) 1170
BtsIMutI CAGTG 3 cut(s) 987, 1038, 1170
Cac8I GCNNGC 2 cut(s) 679, 875
CaiI CAGNNNCTG 2 cut(s) 419, 1100
CfoI GCGC 1 cut(s) 1038
Cfr10I RCCGGY 2 cut(s) 310, 338
Cfr13I GGNCC 5 cut(s) 4, 323, 724, 781, 886
CseI GACGC 2 cut(s) 271, 955
CsiI ACCWGGT 1 cut(s) 783
Csp6I GTAC 3 cut(s) 303, 734, 848
CspAI ACCGGT 1 cut(s) 338
CspCI CAANNNNNGTGG 2 cut(s) 1086, 1121
CviAII CATG 2 cut(s) 747, 916
CviQI GTAC 3 cut(s) 303, 734, 848
DdeI CTNAG 5 cut(s) 201, 384, 456, 492, 582
DpnI GATC 8 cut(s) 95, 102, 186, 375, 549, 815, 1128, 1225
DpnII GATC 8 cut(s) 93, 100, 184, 373, 547, 813, 1126, 1223
Eam1104I CTCTTC 2 cut(s) 44, 537
EarI CTCTTC 2 cut(s) 44, 537
EciI GGCGGA 1 cut(s) 159
Eco24I GRGCYC 1 cut(s) 236
Eco47I GGWCC 5 cut(s) 4, 323, 724, 781, 886
Eco57I CTGAAG 3 cut(s) 128, 921, 1077
EcoO109I RGGNCCY 1 cut(s) 781
EcoRII CCWGG 5 cut(s) 526, 776, 783, 1014, 1174
EcoT38I GRGCYC 1 cut(s) 236
Esp3I CGTCTC 1 cut(s) 52
FaeI CATG 2 cut(s) 750, 919
FaiI YATR 9 cut(s) 84, 671, 748, 807, 827, 917, 933, 964, 1199
FaqI GGGAC 1 cut(s) 794
FatI CATG 2 cut(s) 746, 915
FauI CCCGC 1 cut(s) 253
Fnu4HI GCNGC 2 cut(s) 634, 1012
FokI GGATG 3 cut(s) 1012, 1151, 1163
FriOI GRGCYC 1 cut(s) 236
Fsp4HI GCNGC 2 cut(s) 634, 1012
GlaI GCGC 1 cut(s) 1037
GluI GCNGC 2 cut(s) 634, 1012
GsuI CTGGAG 4 cut(s) 396, 549, 1062, 1197
HgaI GACGC 2 cut(s) 271, 955
HhaI GCGC 1 cut(s) 1038
Hin1I GRCGYC 2 cut(s) 263, 947
Hin1II CATG 2 cut(s) 750, 919
Hin6I GCGC 1 cut(s) 1036
HinP1I GCGC 1 cut(s) 1036
HphI GGTGA 4 cut(s) 67, 110, 799, 1164
Hpy166II GTNNAC 1 cut(s) 326
Hpy188I TCNGA 5 cut(s) 52, 108, 121, 258, 901
Hpy188III TCNNGA 8 cut(s) 278, 383, 413, 449, 485, 566, 970, 1061
Hpy8I GTNNAC 1 cut(s) 326
HpyCH4III ACNGT 2 cut(s) 302, 982
HpyCH4IV ACGT 1 cut(s) 653
HpyCH4V TGCA 8 cut(s) 557, 657, 681, 767, 793, 962, 1163, 1220
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 245, 687
HpyF3I CTNAG 5 cut(s) 201, 384, 456, 492, 582
HpySE526I ACGT 1 cut(s) 653
Hsp92I GRCGYC 2 cut(s) 263, 947
Hsp92II CATG 2 cut(s) 750, 919
HspAI GCGC 1 cut(s) 1036
Kpn2I TCCGGA 3 cut(s) 277, 448, 484
Kzo9I GATC 8 cut(s) 93, 100, 184, 373, 547, 813, 1126, 1223
LguI GCTCTTC 1 cut(s) 44
LmnI GCTCC 4 cut(s) 280, 319, 451, 487
Lsp1109I GCAGC 2 cut(s) 620, 998
LweI GCATC 3 cut(s) 60, 780, 1192
MabI ACCWGGT 1 cut(s) 783
MaeII ACGT 1 cut(s) 653
MaeIII GTNAC 6 cut(s) 593, 601, 943, 976, 1108, 1170
MalI GATC 8 cut(s) 95, 102, 186, 375, 549, 815, 1128, 1225
MboI GATC 8 cut(s) 93, 100, 184, 373, 547, 813, 1126, 1223
MboII GAAGA 8 cut(s) 31, 232, 271, 361, 379, 554, 589, 782
MflI RGATCY 3 cut(s) 373, 547, 1223
MhlI GDGCHC 1 cut(s) 236
MluCI AATT 2 cut(s) 115, 987
MlyI GAGTC 2 cut(s) 247, 556
MmeI TCCRAC 3 cut(s) 467, 503, 539
MnlI CCTC 8 cut(s) 114, 177, 538, 989, 1017, 1020, 1037, 1172
MroI TCCGGA 3 cut(s) 277, 448, 484
MroXI GAANNNNTTC 1 cut(s) 760
MseI TTAA 1 cut(s) 684
MspR9I CCNGG 6 cut(s) 138, 528, 778, 785, 1016, 1176
Mva1269I GAATGC 2 cut(s) 622, 875
MvaI CCWGG 5 cut(s) 528, 778, 785, 1016, 1176
MvnI CGCG 1 cut(s) 738
MwoI GCNNNNNNNGC 3 cut(s) 79, 245, 687
NciI CCSGG 1 cut(s) 138
NdeII GATC 8 cut(s) 93, 100, 184, 373, 547, 813, 1126, 1223
NlaIII CATG 2 cut(s) 750, 919
NlaIV GGNNCC 9 cut(s) 282, 315, 375, 453, 489, 525, 549, 782, 888
NmuCI GTSAC 4 cut(s) 593, 601, 943, 1170
PciSI GCTCTTC 1 cut(s) 44
PcsI WCGNNNNNNNCGW 1 cut(s) 269
PctI GAATGC 2 cut(s) 622, 875
PdmI GAANNNNTTC 1 cut(s) 760
PfeI GAWTC 8 cut(s) 146, 160, 274, 439, 475, 511, 701, 1085
PflMI CCANNNNNTGG 1 cut(s) 956
PinAI ACCGGT 1 cut(s) 338
PkrI GCNGC 2 cut(s) 635, 1013
PleI GAGTC 2 cut(s) 247, 556
PpsI GAGTC 2 cut(s) 247, 556
PpuMI RGGWCCY 1 cut(s) 781
Psp5II RGGWCCY 1 cut(s) 781
Psp6I CCWGG 5 cut(s) 526, 776, 783, 1014, 1174
PspGI CCWGG 5 cut(s) 526, 776, 783, 1014, 1174
PspN4I GGNNCC 9 cut(s) 282, 315, 375, 453, 489, 525, 549, 782, 888
PspPI GGNCC 5 cut(s) 4, 323, 724, 781, 886
PspPPI RGGWCCY 1 cut(s) 781
PstI CTGCAG 1 cut(s) 769
PstNI CAGNNNCTG 2 cut(s) 419, 1100
PsuI RGATCY 3 cut(s) 373, 547, 1223
RsaI GTAC 3 cut(s) 304, 735, 849
RsaNI GTAC 3 cut(s) 303, 734, 848
SapI GCTCTTC 1 cut(s) 44
SaqAI TTAA 1 cut(s) 684
SatI GCNGC 2 cut(s) 634, 1012
Sau3AI GATC 8 cut(s) 93, 100, 184, 373, 547, 813, 1126, 1223
Sau96I GGNCC 5 cut(s) 4, 323, 724, 781, 886
ScaI AGTACT 2 cut(s) 304, 849
SchI GAGTC 2 cut(s) 247, 556
ScrFI CCNGG 6 cut(s) 138, 528, 778, 785, 1016, 1176
SduI GDGCHC 1 cut(s) 236
SexAI ACCWGGT 1 cut(s) 783
SfaNI GCATC 3 cut(s) 60, 780, 1192
SfcI CTRYAG 1 cut(s) 765
SinI GGWCC 5 cut(s) 4, 323, 724, 781, 886
SmlI CTYRAG 1 cut(s) 878
SmoI CTYRAG 1 cut(s) 878
Sse9I AATT 2 cut(s) 115, 987
SsiI CCGC 3 cut(s) 23, 170, 246
StyD4I CCNGG 6 cut(s) 136, 526, 776, 783, 1014, 1174
TaaI ACNGT 2 cut(s) 302, 982
TaiI ACGT 1 cut(s) 656
TaqI TCGA 1 cut(s) 68
TaqII GACCGA 1 cut(s) 21
TasI AATT 2 cut(s) 115, 987
TatI WGTACW 2 cut(s) 302, 847
TfiI GAWTC 8 cut(s) 146, 160, 274, 439, 475, 511, 701, 1085
Tru1I TTAA 1 cut(s) 684
Tru9I TTAA 1 cut(s) 684
TscAI CASTG 3 cut(s) 987, 1045, 1170
TseFI GTSAC 4 cut(s) 593, 601, 943, 1170
TseI GCWGC 2 cut(s) 633, 1011
Tsp45I GTSAC 4 cut(s) 593, 601, 943, 1170
TspDTI ATGAA 2 cut(s) 31, 904
TspGWI ACGGA 2 cut(s) 680, 768
TspRI CASTG 3 cut(s) 987, 1045, 1170
Van91I CCANNNNNTGG 1 cut(s) 956
VpaK11BI GGWCC 5 cut(s) 4, 323, 724, 781, 886
XmnI GAANNNNTTC 1 cut(s) 760
ZrmI AGTACT 2 cut(s) 304, 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.