MD12G1188500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011413
Physical Location & Seq
Forward (+)
27031585 .. 27035501
3917 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1188500.v1.1.491

Sequence Viewer

Length: 1224 bp
ATGGATAGAATCGTTTTTCTGGCGGTGTTTTTCATCGTCTTCGTCGTCGCCGATGTTTCCGATGCTGCTGCTGCGCTCTCCAAGCACAGAATTTTGATTGGTGAGCCTCAAAACGACAATACGACAGTACCAGGCAAGGAGGGTTCTCCGGTGCCTAGTCCGCTGCTGCCTCCTACGGTGTCAAGTTCTGGTGGAAATGGGTCGGATACTAAGCCGGTTGATGACCCGAGTAAGGAGAAAGTGGAGTCTTCGCAACCCGCGAAGACTCCGAAACCGAAAGTGGATCAGGGCCCCAAGGTGGGTTTGAATAATGATACTAAAAAGGGTGAAGGGAAGGATGTGGAAACAGAAAAGAAGCATCCAGAGCCTGCCAAGACATCGAATGATGATCCGAAGCCGGATACCGACAAGGTCAAATATAATCCAAAGGAGGACACTGACAAGGTGTTGAAAGATAATCCTAAGGAGAAGGAGAAGGATAATCCAAAGGAGAAGGAGAAGGAGAAGGAGAAGGATAATCCAAAGGAGAAGGAGAAGGATAATCCGAAGGAGAAGGAGAAGGAGAAGGATAATCCAAGGGAGAAGGAGAAGGAGAAGCAGGAGAGCTGCGCTGGGGTAACCAAAATCAAAACATGTGCGGTCAAGGATGTGGCAGTTGCTTGCATTAAGAACTTCAGTAGTGGGTCCAAAGAAGTGGCCATTCTGGTTCAAAACGTAGGAAACAGCACTTTGAACGCAACCCTTTCTGCAAATGGTGCCAGCAAGGTCCTAGAGATTCTGAAACACCTAAATAGAAAGGTCAATATATCAATTGATCCGGGTAAAAGCACCAATATAACATTAAATACTGGAACCGGAGAATGTAAGCTTCATATAGATCCTCCCCCTCCGGCGTTTCCTTCTCTCAAGACCATAGCGACCCCAATCAATGGTGCCTACTTCTTGATTGTTGCAGTGCTAACTTTTGGAGGAACGTGGGCTTGCTGCTTGATCAGGAAGGGGAAAGAGCGTACTGGAGGTGGAGTTCCATATCAGGAACTTGAAATGGGATTGCCGGAATCTGTTCCAGCAACCGCCGTGGAAACGGCTGAAGGTTGGGATGAAGGTTGGGATGATGACTGGGATGGAGAAAATGCGGTGAAAACACCTGGGGATCGTCTGGGAAGCATCTCTGTGAATGGCCTTACATCGAGGGCTGCAAACAAAGATGGGTGGAATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

408

Amino Acids

43.89

Weight (kDa)

5.73

Isoelectric Point (pI)

27.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7356 PF24053 195 - 295 7.7e-23 Domain of unknown function (DUF7356)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011413)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51580 AT3G51580
fragaria_vesca FvH4_6g09850
malus_domestica MD04G1174800.v1.1 MD12G1188500.v1.1
prunus_persica Prupe.6G293800_v2.0.a1
pyrus_communis pycom04g15420 pycom12g17610
rosa_chinensis RchiOBHm_Chr3g0460451
rosa_laevigata RLG00000025010
rosa_multiflora Rmu_sc0004267.1_g000009 Rmu_sc0021028.1_g000003
rosa_roxburghii Rroxscaffold_6G00419470
rosa_rugosa Rorug03G0042600 Rorug03G0042700
rosa_samantha Rh3AG100800 Rh3BG104800 Rh3CG106600 Rh3DG106100
rosa_wichuraiana Rw3G008580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 151, 755, 932
AccB7I CCANNNNNTGG 1 cut(s) 929
AccII CGCG 1 cut(s) 260
AciI CCGC 6 cut(s) 23, 161, 258, 638, 1074, 1136
AclWI GGATC 5 cut(s) 291, 383, 809, 872, 1161
AcoI YGGCCR 1 cut(s) 696
AcsI RAATTY 1 cut(s) 90
AcuI CTGAAG 2 cut(s) 658, 1110
AfaI GTAC 2 cut(s) 129, 1012
AfiI CCNNNNNNNGG 4 cut(s) 232, 298, 299, 929
AflIII ACRYGT 1 cut(s) 632
AgsI TTSAA 5 cut(s) 307, 451, 710, 733, 1043
AjnI CCWGG 2 cut(s) 130, 1147
AloI GAACNNNNNNTCC 2 cut(s) 1008, 1040
AluBI AGCT 2 cut(s) 606, 868
AluI AGCT 2 cut(s) 606, 868
AlwI GGATC 5 cut(s) 291, 383, 809, 872, 1161
AlwNI CAGNNNCTG 1 cut(s) 368
Ama87I CYCGRG 1 cut(s) 226
AoxI GGCC 3 cut(s) 289, 696, 1180
ApaI GGGCCC 1 cut(s) 293
ApeKI GCWGC 8 cut(s) 65, 68, 71, 163, 166, 606, 984, 1196
ApoI RAATTY 1 cut(s) 90
Asp700I GAANNNNTTC 1 cut(s) 1062
AspLEI GCGC 2 cut(s) 76, 611
AspS9I GGNCC 4 cut(s) 289, 290, 684, 766
AsuC2I CCSGG 1 cut(s) 819
AsuHPI GGTGA 3 cut(s) 113, 338, 1150
AvaI CYCGRG 1 cut(s) 226
AvaII GGWCC 2 cut(s) 684, 766
AxyI CCTNAGG 1 cut(s) 462
BaeGI GKGCMC 1 cut(s) 293
BaeI ACNNNNGTAYC 2 cut(s) 111, 144
BalI TGGCCA 1 cut(s) 698
BanI GGYRCC 3 cut(s) 151, 755, 932
BanII GRGCYC 1 cut(s) 293
BbsI GAAGAC 3 cut(s) 31, 240, 269
BbvI GCAGC 8 cut(s) 52, 55, 58, 150, 153, 593, 971, 1183
BccI CCATC 2 cut(s) 1118, 1202
BceAI ACGGC 2 cut(s) 1061, 1101
BcgI CGANNNNNNTGC 2 cut(s) 50, 84
BciT130I CCWGG 2 cut(s) 132, 1149
BciVI GTATCC 2 cut(s) 199, 394
BclI TGATCA 1 cut(s) 990
BcnI CCSGG 1 cut(s) 819
BfaI CTAG 2 cut(s) 156, 770
BfuI GTATCC 2 cut(s) 199, 394
BisI GCNGC 8 cut(s) 66, 69, 72, 164, 167, 607, 985, 1197
BlsI GCNGC 8 cut(s) 67, 70, 73, 165, 168, 608, 986, 1198
Bme1390I CCNGG 3 cut(s) 132, 819, 1149
Bme18I GGWCC 2 cut(s) 684, 766
BmeT110I CYCGRG 1 cut(s) 226
BmgT120I GGNCC 4 cut(s) 289, 290, 684, 766
BmiI GGNNCC 7 cut(s) 153, 291, 292, 685, 757, 853, 934
BmrFI CCNGG 3 cut(s) 132, 819, 1149
BmrI ACTGGG 1 cut(s) 1129
BmsI GCATC 3 cut(s) 52, 367, 1176
BmuI ACTGGG 1 cut(s) 1129
BpiI GAAGAC 3 cut(s) 31, 240, 269
BpmI CTGGAG 1 cut(s) 1035
BpuEI CTTGAG 1 cut(s) 890
BpuMI CCSGG 1 cut(s) 819
BsaJI CCNNGG 4 cut(s) 294, 575, 1077, 1148
BsaWI WCCGGW 2 cut(s) 148, 854
BsaXI ACNNNNNCTCC 2 cut(s) 1008, 1038
Bsc4I CCNNNNNNNGG 4 cut(s) 232, 298, 299, 929
Bse118I RCCGGY 1 cut(s) 214
Bse1I ACTGG 3 cut(s) 853, 1018, 1124
Bse21I CCTNAGG 1 cut(s) 462
BseBI CCWGG 2 cut(s) 132, 1149
BseDI CCNNGG 4 cut(s) 294, 575, 1077, 1148
BseGI GGATG 6 cut(s) 343, 358, 652, 1105, 1117, 1129
BseLI CCNNNNNNNGG 4 cut(s) 232, 298, 299, 929
BseNI ACTGG 3 cut(s) 853, 1018, 1124
BseSI GKGCMC 1 cut(s) 293
BseXI GCAGC 8 cut(s) 52, 55, 58, 150, 153, 593, 971, 1183
BseYI CCCAGC 1 cut(s) 611
Bsh1236I CGCG 1 cut(s) 260
BshFI GGCC 3 cut(s) 291, 698, 1182
BshNI GGYRCC 3 cut(s) 151, 755, 932
BsiHKCI CYCGRG 1 cut(s) 226
BsiSI CCGG 7 cut(s) 149, 215, 398, 818, 855, 890, 1055
BslI CCNNNNNNNGG 4 cut(s) 232, 298, 299, 929
BsnI GGCC 3 cut(s) 291, 698, 1182
BsoBI CYCGRG 1 cut(s) 226
Bsp120I GGGCCC 1 cut(s) 289
Bsp1286I GDGCHC 1 cut(s) 293
Bsp143I GATC 6 cut(s) 283, 388, 814, 877, 990, 1153
BspACI CCGC 6 cut(s) 23, 161, 258, 638, 1074, 1136
BspANI GGCC 3 cut(s) 291, 698, 1182
BspFNI CGCG 1 cut(s) 260
BspLI GGNNCC 7 cut(s) 153, 291, 292, 685, 757, 853, 934
BspPI GGATC 5 cut(s) 291, 383, 809, 872, 1161
BspT107I GGYRCC 3 cut(s) 151, 755, 932
BsrFI RCCGGY 1 cut(s) 214
BsrI ACTGG 3 cut(s) 853, 1018, 1124
BssAI RCCGGY 1 cut(s) 214
BssECI CCNNGG 4 cut(s) 294, 575, 1077, 1148
BssMI GATC 6 cut(s) 283, 388, 814, 877, 990, 1153
BssT1I CCWWGG 2 cut(s) 294, 575
Bst2UI CCWGG 2 cut(s) 132, 1149
Bst4CI ACNGT 2 cut(s) 127, 178
BstAPI GCANNNNNTGC 1 cut(s) 755
BstC8I GCNNGC 4 cut(s) 369, 661, 760, 982
BstDEI CTNAG 2 cut(s) 210, 462
BstDSI CCRYGG 1 cut(s) 1077
BstEII GGTNACC 1 cut(s) 616
BstF5I GGATG 6 cut(s) 343, 358, 652, 1105, 1117, 1129
BstFNI CGCG 1 cut(s) 260
BstHHI GCGC 2 cut(s) 76, 611
BstKTI GATC 6 cut(s) 286, 391, 817, 880, 993, 1156
BstMBI GATC 6 cut(s) 283, 388, 814, 877, 990, 1153
BstMWI GCNNNNNNNGC 5 cut(s) 71, 82, 160, 364, 755
BstNI CCWGG 2 cut(s) 132, 1149
BstNSI RCATGY 1 cut(s) 636
BstPI GGTNACC 1 cut(s) 616
BstSCI CCNGG 3 cut(s) 130, 817, 1147
BstSLI GKGCMC 1 cut(s) 293
BstUI CGCG 1 cut(s) 260
BstV1I GCAGC 8 cut(s) 52, 55, 58, 150, 153, 593, 971, 1183
BstV2I GAAGAC 3 cut(s) 31, 240, 269
BstX2I RGATCY 1 cut(s) 877
BstXI CCANNNNNNTGG 1 cut(s) 694
BstYI RGATCY 1 cut(s) 877
Bsu36I CCTNAGG 1 cut(s) 462
BsuI GTATCC 2 cut(s) 199, 394
BsuRI GGCC 3 cut(s) 291, 698, 1182
BtgI CCRYGG 1 cut(s) 1077
BtsCI GGATG 6 cut(s) 343, 358, 652, 1105, 1117, 1129
BtsI GCAGTG 1 cut(s) 960
BtsIMutI CAGTG 2 cut(s) 435, 960
Cac8I GCNNGC 4 cut(s) 369, 661, 760, 982
CaiI CAGNNNCTG 1 cut(s) 368
CfoI GCGC 2 cut(s) 76, 611
Cfr10I RCCGGY 1 cut(s) 214
Cfr13I GGNCC 4 cut(s) 289, 290, 684, 766
Csp6I GTAC 2 cut(s) 128, 1011
CspCI CAANNNNNGTGG 2 cut(s) 1059, 1094
CviAII CATG 1 cut(s) 633
CviQI GTAC 2 cut(s) 128, 1011
DdeI CTNAG 2 cut(s) 210, 462
DpnI GATC 6 cut(s) 285, 390, 816, 879, 992, 1155
DpnII GATC 6 cut(s) 283, 388, 814, 877, 990, 1153
EaeI YGGCCR 1 cut(s) 696
Eco130I CCWWGG 2 cut(s) 294, 575
Eco24I GRGCYC 1 cut(s) 293
Eco47I GGWCC 2 cut(s) 684, 766
Eco57I CTGAAG 2 cut(s) 658, 1110
Eco81I CCTNAGG 1 cut(s) 462
Eco88I CYCGRG 1 cut(s) 226
Eco91I GGTNACC 1 cut(s) 616
EcoO109I RGGNCCY 3 cut(s) 289, 290, 766
EcoO65I GGTNACC 1 cut(s) 616
EcoRII CCWGG 2 cut(s) 130, 1147
EcoT14I CCWWGG 2 cut(s) 294, 575
EcoT38I GRGCYC 1 cut(s) 293
ErhI CCWWGG 2 cut(s) 294, 575
FaeI CATG 1 cut(s) 636
FaiI YATR 8 cut(s) 420, 634, 806, 836, 873, 875, 914, 1030
FatI CATG 1 cut(s) 632
FauI CCCGC 1 cut(s) 265
FbaI TGATCA 1 cut(s) 990
Fnu4HI GCNGC 8 cut(s) 66, 69, 72, 164, 167, 607, 985, 1197
FokI GGATG 6 cut(s) 345, 350, 659, 1112, 1124, 1136
FriOI GRGCYC 1 cut(s) 293
Fsp4HI GCNGC 8 cut(s) 66, 69, 72, 164, 167, 607, 985, 1197
FspBI CTAG 2 cut(s) 156, 770
GlaI GCGC 2 cut(s) 75, 610
GluI GCNGC 8 cut(s) 66, 69, 72, 164, 167, 607, 985, 1197
GsaI CCCAGC 1 cut(s) 615
GsuI CTGGAG 1 cut(s) 1035
HaeIII GGCC 3 cut(s) 291, 698, 1182
HapII CCGG 7 cut(s) 149, 215, 398, 818, 855, 890, 1055
HhaI GCGC 2 cut(s) 76, 611
Hin1II CATG 1 cut(s) 636
Hin6I GCGC 2 cut(s) 74, 609
HinP1I GCGC 2 cut(s) 74, 609
HindIII AAGCTT 1 cut(s) 866
HinfI GANTC 5 cut(s) 9, 245, 265, 775, 1058
HpaII CCGG 7 cut(s) 149, 215, 398, 818, 855, 890, 1055
HphI GGTGA 3 cut(s) 113, 338, 1150
Hpy188I TCNGA 6 cut(s) 61, 205, 270, 393, 546, 780
Hpy188III TCNNGA 5 cut(s) 362, 907, 943, 994, 1034
Hpy99I CGWCG 2 cut(s) 47, 50
HpyCH4III ACNGT 2 cut(s) 127, 178
HpyCH4IV ACGT 2 cut(s) 714, 974
HpyCH4V TGCA 4 cut(s) 663, 749, 953, 1199
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 82, 160, 364, 755
HpyF3I CTNAG 2 cut(s) 210, 462
HpySE526I ACGT 2 cut(s) 714, 974
Hsp92II CATG 1 cut(s) 636
HspAI GCGC 2 cut(s) 74, 609
Ksp22I TGATCA 1 cut(s) 990
Kzo9I GATC 6 cut(s) 283, 388, 814, 877, 990, 1153
Lsp1109I GCAGC 8 cut(s) 52, 55, 58, 150, 153, 593, 971, 1183
LweI GCATC 3 cut(s) 52, 367, 1176
MaeI CTAG 2 cut(s) 156, 770
MaeII ACGT 2 cut(s) 714, 974
MaeIII GTNAC 1 cut(s) 616
MalI GATC 6 cut(s) 285, 390, 816, 879, 992, 1155
MboI GATC 6 cut(s) 283, 388, 814, 877, 990, 1153
MboII GAAGA 3 cut(s) 31, 240, 274
MfeI CAATTG 1 cut(s) 810
MflI RGATCY 1 cut(s) 877
MhlI GDGCHC 1 cut(s) 293
MlsI TGGCCA 1 cut(s) 698
MluCI AATT 2 cut(s) 90, 810
MluNI TGGCCA 1 cut(s) 698
MlyI GAGTC 2 cut(s) 254, 259
MmeI TCCRAC 1 cut(s) 183
MnlI CCTC 9 cut(s) 117, 133, 180, 424, 891, 897, 962, 1010, 1185
Mox20I TGGCCA 1 cut(s) 698
MroXI GAANNNNTTC 1 cut(s) 1062
MscI TGGCCA 1 cut(s) 698
MseI TTAA 2 cut(s) 666, 842
MslI CAYNNNNRTG 1 cut(s) 1172
Msp20I TGGCCA 1 cut(s) 698
MspA1I CMGCKG 1 cut(s) 163
MspI CCGG 7 cut(s) 149, 215, 398, 818, 855, 890, 1055
MspR9I CCNGG 3 cut(s) 132, 819, 1149
MunI CAATTG 1 cut(s) 810
MvaI CCWGG 2 cut(s) 132, 1149
MvnI CGCG 1 cut(s) 260
MwoI GCNNNNNNNGC 5 cut(s) 71, 82, 160, 364, 755
NciI CCSGG 1 cut(s) 819
NdeII GATC 6 cut(s) 283, 388, 814, 877, 990, 1153
NlaIII CATG 1 cut(s) 636
NlaIV GGNNCC 7 cut(s) 153, 291, 292, 685, 757, 853, 934
NspI RCATGY 1 cut(s) 636
PciI ACATGT 1 cut(s) 632
PcsI WCGNNNNNNNCGW 3 cut(s) 42, 48, 257
PdmI GAANNNNTTC 1 cut(s) 1062
PfeI GAWTC 3 cut(s) 9, 775, 1058
PflFI GACNNNGTC 1 cut(s) 410
PflMI CCANNNNNTGG 1 cut(s) 929
PkrI GCNGC 8 cut(s) 67, 70, 73, 165, 168, 608, 986, 1198
PleI GAGTC 2 cut(s) 253, 259
PpsI GAGTC 2 cut(s) 253, 259
PpuMI RGGWCCY 1 cut(s) 766
PscI ACATGT 1 cut(s) 632
Psp5II RGGWCCY 1 cut(s) 766
Psp6I CCWGG 2 cut(s) 130, 1147
PspEI GGTNACC 1 cut(s) 616
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 2 cut(s) 130, 1147
PspN4I GGNNCC 7 cut(s) 153, 291, 292, 685, 757, 853, 934
PspOMI GGGCCC 1 cut(s) 289
PspPI GGNCC 4 cut(s) 289, 290, 684, 766
PspPPI RGGWCCY 1 cut(s) 766
PstNI CAGNNNCTG 1 cut(s) 368
PsuI RGATCY 1 cut(s) 877
PsyI GACNNNGTC 1 cut(s) 410
RsaI GTAC 2 cut(s) 129, 1012
RsaNI GTAC 2 cut(s) 128, 1011
RseI CAYNNNNRTG 1 cut(s) 1172
SaqAI TTAA 2 cut(s) 666, 842
SatI GCNGC 8 cut(s) 66, 69, 72, 164, 167, 607, 985, 1197
Sau3AI GATC 6 cut(s) 283, 388, 814, 877, 990, 1153
Sau96I GGNCC 4 cut(s) 289, 290, 684, 766
SchI GAGTC 2 cut(s) 254, 259
ScrFI CCNGG 3 cut(s) 132, 819, 1149
SduI GDGCHC 1 cut(s) 293
SfaNI GCATC 3 cut(s) 52, 367, 1176
SinI GGWCC 2 cut(s) 684, 766
SmiMI CAYNNNNRTG 1 cut(s) 1172
SmlI CTYRAG 1 cut(s) 905
SmoI CTYRAG 1 cut(s) 905
Sse9I AATT 2 cut(s) 90, 810
SsiI CCGC 6 cut(s) 23, 161, 258, 638, 1074, 1136
SspMI CTAG 2 cut(s) 156, 770
StyD4I CCNGG 3 cut(s) 130, 817, 1147
StyI CCWWGG 2 cut(s) 294, 575
TaaI ACNGT 2 cut(s) 127, 178
TaiI ACGT 2 cut(s) 717, 977
TaqI TCGA 2 cut(s) 380, 1190
TasI AATT 2 cut(s) 90, 810
TfiI GAWTC 3 cut(s) 9, 775, 1058
Tru1I TTAA 2 cut(s) 666, 842
Tru9I TTAA 2 cut(s) 666, 842
TscAI CASTG 2 cut(s) 442, 960
TseI GCWGC 8 cut(s) 65, 68, 71, 163, 166, 606, 984, 1196
TspDTI ATGAA 3 cut(s) 22, 860, 1116
TspRI CASTG 2 cut(s) 442, 960
Tth111I GACNNNGTC 1 cut(s) 410
Van91I CCANNNNNTGG 1 cut(s) 929
VpaK11BI GGWCC 2 cut(s) 684, 766
XapI RAATTY 1 cut(s) 90
XceI RCATGY 1 cut(s) 636
XmnI GAANNNNTTC 1 cut(s) 1062
XspI CTAG 2 cut(s) 156, 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.