pycom04g15420

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0011413
Physical Location & Seq
Forward (+)
18311696 .. 18315531
3836 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g15420.2

Sequence Viewer

Length: 1257 bp
ATGGATAGAATCGGTTTTCTGGCGGTGTTCTTCATTTTCTTCGTCGTCGTCGAGGTTTCCGATGCTTCTTATCTTCTCTCCAAGCACAGAGTTTTGATTGGTGAGCCTCAAAACGACACTACGGCAGTACCAGATAACGACAAGGTCAAGGAGGATCCTCCGGTCCCGAGTCCGCTGCTGCCTTCTACGGTGTCAAGTTCTGGTGGAAATGGGTCGGATACTAAGCCGGTTGATGATCCGAGTAAGGACAAAGCGGAGACTCCGCAACCCACGAAGACTACGACACAGATAGTGGATCAGGTCCCCAAGGGGGGCTTGAATAATGATACTAAAAAGGGTGAAGGGAAGGCTCCGGAAACAGAAAAGAAGGATCCGGAAACGGAAAAGAAGAAGCCGGACACTGACAAGGTGGCGAAAGATGATCCAAAGCCGGACACTGACAAGGTGTCTAAAGATGATCCTAAGCTGGTTCATGACAAGTCAAAGGATGATAATGTGACTCGACCCGCGGATGAAGGTGGTAAGAAAAAGAGTAAGGAGAAGGAGAAGAAGAAGAAGAAGAAGAATGATGATGTGACCCAATTGCCAAAAGAGGATAAGGAAAGCTGTAATGGGGTAATAAAAAAATGTGAGGTCAAGGATGTGGCGATTGCTTGCATTAAGAGCTTCGATAGTGGGTCCAAAGAAGTGGCCATTCTGGTCCAAAACATAGGAGACAGCACTTTGAAGGCAAAGCTTTCTGCAGATGATACCAACAAGGACCTAGAGATCCTCAAACACAAAAATAAAAAGGTCAATATATCAGTTGATATGGGCAAAAGCACCACCAAAATAACACTAACTTCTGGAAATGGAGAATGTGAGCTTCTTATGGATCCTCTCCCTCTGGCATATGAAGGAAACTTATTCATGCGTTTCCCTTCACTCGAGACTGTAGCGACCCCGATCAATGCTGCCTACTTCTTGATTATTACGGTGCTAATTTTTGGAGGAACGTGGGCTTGCTGCTTGGTCAGGAAGAGGAAAGAGCGTACTGGAGGTGGAGTTCCATATCAGGAACTCGAAATGGGATTGCCGGAATCTGTTTCAGCAACTGCTGTGGAAACGGCTGAAGGTTGGGACGAAGGTTGGGATGATGATTGGGATGGCGATAATGCGGTGAAAACACCTGGGACGCATCTGGGAAGCATCTCTGCGAATGGCCTTACATCGAGAACCGCAAACAAGGATGGGTGGGATAATGATTGGGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

419

Amino Acids

45.54

Weight (kDa)

5.14

Isoelectric Point (pI)

26.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011413)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51580 AT3G51580
fragaria_vesca FvH4_6g09850
malus_domestica MD04G1174800.v1.1 MD12G1188500.v1.1
prunus_persica Prupe.6G293800_v2.0.a1
pyrus_communis pycom04g15420 pycom12g17610
rosa_chinensis RchiOBHm_Chr3g0460451
rosa_laevigata RLG00000025010
rosa_multiflora Rmu_sc0004267.1_g000009 Rmu_sc0021028.1_g000003
rosa_roxburghii Rroxscaffold_6G00419470
rosa_rugosa Rorug03G0042600 Rorug03G0042700
rosa_samantha Rh3AG100800 Rh3BG104800 Rh3CG106600 Rh3DG106100
rosa_wichuraiana Rw3G008580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 509
AccIII TCCGGA 2 cut(s) 352, 373
AciI CCGC 8 cut(s) 23, 173, 254, 263, 507, 509, 1157, 1218
AcoI YGGCCR 1 cut(s) 690
AcuI CTGAAG 1 cut(s) 1131
AfaI GTAC 2 cut(s) 129, 1033
AfiI CCNNNNNNNGG 2 cut(s) 310, 311
AgsI TTSAA 2 cut(s) 319, 727
AhdI GACNNNNNGTC 1 cut(s) 445
AjnI CCWGG 1 cut(s) 1168
AloI GAACNNNNNNTCC 2 cut(s) 1029, 1061
AluBI AGCT 5 cut(s) 466, 606, 666, 736, 865
AluI AGCT 5 cut(s) 466, 606, 666, 736, 865
Alw26I GTCTC 3 cut(s) 251, 708, 923
AlwNI CAGNNNCTG 1 cut(s) 1094
Ama87I CYCGRG 2 cut(s) 166, 926
Aor13HI TCCGGA 2 cut(s) 352, 373
AoxI GGCC 2 cut(s) 690, 1201
ApeKI GCWGC 4 cut(s) 175, 178, 953, 1005
AspS9I GGNCC 5 cut(s) 163, 301, 678, 700, 760
AsuHPI GGTGA 3 cut(s) 113, 350, 1171
AvaI CYCGRG 2 cut(s) 166, 926
AvaII GGWCC 5 cut(s) 163, 301, 678, 700, 760
BaeI ACNNNNGTAYC 2 cut(s) 111, 144
BalI TGGCCA 1 cut(s) 692
BamHI GGATCC 3 cut(s) 154, 370, 874
BbsI GAAGAC 1 cut(s) 281
BbvI GCAGC 4 cut(s) 162, 165, 940, 992
BccI CCATC 2 cut(s) 1139, 1223
BceAI ACGGC 2 cut(s) 138, 1122
BcgI CGANNNNNNTGC 2 cut(s) 157, 191
BciT130I CCWGG 1 cut(s) 1170
BciVI GTATCC 1 cut(s) 211
BcoDI GTCTC 3 cut(s) 251, 708, 923
BfaI CTAG 1 cut(s) 764
BfmI CTRYAG 2 cut(s) 741, 933
BfuI GTATCC 1 cut(s) 211
BisI GCNGC 4 cut(s) 176, 179, 954, 1006
BlsI GCNGC 4 cut(s) 177, 180, 955, 1007
Bme1390I CCNGG 1 cut(s) 1170
Bme18I GGWCC 5 cut(s) 163, 301, 678, 700, 760
BmeRI GACNNNNNGTC 1 cut(s) 445
BmeT110I CYCGRG 2 cut(s) 166, 926
BmgT120I GGNCC 5 cut(s) 163, 301, 678, 700, 760
BmiI GGNNCC 7 cut(s) 156, 165, 303, 351, 372, 679, 876
BmrFI CCNGG 1 cut(s) 1170
BmsI GCATC 3 cut(s) 52, 1186, 1197
BpiI GAAGAC 1 cut(s) 281
BpmI CTGGAG 1 cut(s) 1056
Bpu10I CCTNAGC 1 cut(s) 462
BsaJI CCNNGG 3 cut(s) 306, 507, 1169
BsaWI WCCGGW 3 cut(s) 160, 352, 373
BsaXI ACNNNNNCTCC 2 cut(s) 1029, 1059
Bsc4I CCNNNNNNNGG 2 cut(s) 310, 311
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 1 cut(s) 1039
BseAI TCCGGA 2 cut(s) 352, 373
BseBI CCWGG 1 cut(s) 1170
BseDI CCNNGG 3 cut(s) 306, 507, 1169
BseGI GGATG 7 cut(s) 493, 517, 646, 1138, 1150, 1234, 1255
BseLI CCNNNNNNNGG 2 cut(s) 310, 311
BseNI ACTGG 1 cut(s) 1039
BseXI GCAGC 4 cut(s) 162, 165, 940, 992
Bsh1236I CGCG 1 cut(s) 509
BshFI GGCC 2 cut(s) 692, 1203
BsiHKCI CYCGRG 2 cut(s) 166, 926
BsiSI CCGG 7 cut(s) 161, 227, 353, 374, 395, 431, 1076
BslFI GGGAC 4 cut(s) 149, 287, 1133, 1186
BslI CCNNNNNNNGG 2 cut(s) 310, 311
BsmAI GTCTC 3 cut(s) 251, 708, 923
BsmFI GGGAC 4 cut(s) 149, 287, 1133, 1186
BsnI GGCC 2 cut(s) 692, 1203
BsoBI CYCGRG 2 cut(s) 166, 926
Bsp13I TCCGGA 2 cut(s) 352, 373
Bsp143I GATC 9 cut(s) 154, 235, 295, 370, 421, 457, 768, 874, 945
BspACI CCGC 8 cut(s) 23, 173, 254, 263, 507, 509, 1157, 1218
BspANI GGCC 2 cut(s) 692, 1203
BspEI TCCGGA 2 cut(s) 352, 373
BspFNI CGCG 1 cut(s) 509
BspHI TCATGA 1 cut(s) 472
BspLI GGNNCC 7 cut(s) 156, 165, 303, 351, 372, 679, 876
BspMAI CTGCAG 1 cut(s) 745
BsrFI RCCGGY 1 cut(s) 226
BsrI ACTGG 1 cut(s) 1039
BssAI RCCGGY 1 cut(s) 226
BssECI CCNNGG 3 cut(s) 306, 507, 1169
BssMI GATC 9 cut(s) 154, 235, 295, 370, 421, 457, 768, 874, 945
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 1170
Bst4CI ACNGT 3 cut(s) 190, 934, 976
Bst6I CTCTTC 1 cut(s) 1013
BstC8I GCNNGC 2 cut(s) 655, 1003
BstDEI CTNAG 2 cut(s) 222, 462
BstDSI CCRYGG 1 cut(s) 507
BstF5I GGATG 7 cut(s) 493, 517, 646, 1138, 1150, 1234, 1255
BstFNI CGCG 1 cut(s) 509
BstKTI GATC 9 cut(s) 157, 238, 298, 373, 424, 460, 771, 877, 948
BstMAI GTCTC 3 cut(s) 251, 708, 923
BstMBI GATC 9 cut(s) 154, 235, 295, 370, 421, 457, 768, 874, 945
BstMWI GCNNNNNNNGC 1 cut(s) 663
BstNI CCWGG 1 cut(s) 1170
BstSCI CCNGG 1 cut(s) 1168
BstSFI CTRYAG 2 cut(s) 741, 933
BstUI CGCG 1 cut(s) 509
BstV1I GCAGC 4 cut(s) 162, 165, 940, 992
BstV2I GAAGAC 1 cut(s) 281
BstX2I RGATCY 4 cut(s) 154, 370, 768, 874
BstXI CCANNNNNNTGG 1 cut(s) 688
BstYI RGATCY 4 cut(s) 154, 370, 768, 874
BsuI GTATCC 1 cut(s) 211
BsuRI GGCC 2 cut(s) 692, 1203
BtgI CCRYGG 1 cut(s) 507
BtsCI GGATG 7 cut(s) 493, 517, 646, 1138, 1150, 1234, 1255
BtsIMutI CAGTG 2 cut(s) 399, 435
Cac8I GCNNGC 2 cut(s) 655, 1003
CaiI CAGNNNCTG 1 cut(s) 1094
CciI TCATGA 1 cut(s) 472
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 5 cut(s) 163, 301, 678, 700, 760
Cfr42I CCGCGG 1 cut(s) 510
CseI GACGC 1 cut(s) 1183
Csp6I GTAC 2 cut(s) 128, 1032
CspCI CAANNNNNGTGG 2 cut(s) 1080, 1115
CviAII CATG 2 cut(s) 473, 910
CviQI GTAC 2 cut(s) 128, 1032
DdeI CTNAG 2 cut(s) 222, 462
DpnI GATC 9 cut(s) 156, 237, 297, 372, 423, 459, 770, 876, 947
DpnII GATC 9 cut(s) 154, 235, 295, 370, 421, 457, 768, 874, 945
DriI GACNNNNNGTC 1 cut(s) 445
EaeI YGGCCR 1 cut(s) 690
Eam1104I CTCTTC 1 cut(s) 1013
Eam1105I GACNNNNNGTC 1 cut(s) 445
EarI CTCTTC 1 cut(s) 1013
Eco130I CCWWGG 1 cut(s) 306
Eco47I GGWCC 5 cut(s) 163, 301, 678, 700, 760
Eco57I CTGAAG 1 cut(s) 1131
Eco88I CYCGRG 2 cut(s) 166, 926
EcoO109I RGGNCCY 2 cut(s) 301, 760
EcoRII CCWGG 1 cut(s) 1168
EcoT14I CCWWGG 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 306
FaeI CATG 2 cut(s) 476, 913
FaiI YATR 9 cut(s) 474, 710, 800, 812, 872, 892, 894, 911, 1051
FalI AAGNNNNNCTT 2 cut(s) 299, 331
FaqI GGGAC 4 cut(s) 149, 287, 1133, 1186
FatI CATG 2 cut(s) 472, 909
FauI CCCGC 1 cut(s) 514
FauNDI CATATG 1 cut(s) 892
Fnu4HI GCNGC 4 cut(s) 176, 179, 954, 1006
FokI GGATG 6 cut(s) 500, 524, 653, 1145, 1157, 1241
Fsp4HI GCNGC 4 cut(s) 176, 179, 954, 1006
FspBI CTAG 1 cut(s) 764
GluI GCNGC 4 cut(s) 176, 179, 954, 1006
GsuI CTGGAG 1 cut(s) 1056
HaeIII GGCC 2 cut(s) 692, 1203
HapII CCGG 7 cut(s) 161, 227, 353, 374, 395, 431, 1076
HgaI GACGC 1 cut(s) 1183
Hin1II CATG 2 cut(s) 476, 913
HindIII AAGCTT 1 cut(s) 734
HinfI GANTC 5 cut(s) 9, 169, 259, 499, 1079
HpaII CCGG 7 cut(s) 161, 227, 353, 374, 395, 431, 1076
HphI GGTGA 3 cut(s) 113, 350, 1171
Hpy188I TCNGA 3 cut(s) 61, 217, 240
Hpy99I CGWCG 3 cut(s) 47, 50, 53
HpyCH4III ACNGT 3 cut(s) 190, 934, 976
HpyCH4IV ACGT 1 cut(s) 995
HpyCH4V TGCA 2 cut(s) 657, 743
HpyF10VI GCNNNNNNNGC 1 cut(s) 663
HpyF3I CTNAG 2 cut(s) 222, 462
HpySE526I ACGT 1 cut(s) 995
Hsp92II CATG 2 cut(s) 476, 913
Kpn2I TCCGGA 2 cut(s) 352, 373
KspI CCGCGG 1 cut(s) 510
Kzo9I GATC 9 cut(s) 154, 235, 295, 370, 421, 457, 768, 874, 945
LmnI GCTCC 1 cut(s) 355
Lsp1109I GCAGC 4 cut(s) 162, 165, 940, 992
LweI GCATC 3 cut(s) 52, 1186, 1197
MaeI CTAG 1 cut(s) 764
MaeII ACGT 1 cut(s) 995
MaeIII GTNAC 2 cut(s) 496, 574
MalI GATC 9 cut(s) 156, 237, 297, 372, 423, 459, 770, 876, 947
MboI GATC 9 cut(s) 154, 235, 295, 370, 421, 457, 768, 874, 945
MfeI CAATTG 1 cut(s) 581
MflI RGATCY 4 cut(s) 154, 370, 768, 874
MlsI TGGCCA 1 cut(s) 692
MluCI AATT 2 cut(s) 581, 981
MluNI TGGCCA 1 cut(s) 692
MlyI GAGTC 3 cut(s) 178, 253, 493
MmeI TCCRAC 1 cut(s) 195
Mox20I TGGCCA 1 cut(s) 692
MroI TCCGGA 2 cut(s) 352, 373
MscI TGGCCA 1 cut(s) 692
MseI TTAA 1 cut(s) 660
Msp20I TGGCCA 1 cut(s) 692
MspA1I CMGCKG 2 cut(s) 175, 509
MspI CCGG 7 cut(s) 161, 227, 353, 374, 395, 431, 1076
MspR9I CCNGG 1 cut(s) 1170
MunI CAATTG 1 cut(s) 581
MvaI CCWGG 1 cut(s) 1170
MvnI CGCG 1 cut(s) 509
MwoI GCNNNNNNNGC 1 cut(s) 663
NdeI CATATG 1 cut(s) 892
NdeII GATC 9 cut(s) 154, 235, 295, 370, 421, 457, 768, 874, 945
NlaIII CATG 2 cut(s) 476, 913
NlaIV GGNNCC 7 cut(s) 156, 165, 303, 351, 372, 679, 876
NmuCI GTSAC 2 cut(s) 496, 574
PaeR7I CTCGAG 1 cut(s) 926
PagI TCATGA 1 cut(s) 472
PcsI WCGNNNNNNNCGW 3 cut(s) 48, 57, 278
PfeI GAWTC 2 cut(s) 9, 1079
PflFI GACNNNGTC 1 cut(s) 143
PkrI GCNGC 4 cut(s) 177, 180, 955, 1007
PleI GAGTC 3 cut(s) 177, 253, 493
PpsI GAGTC 3 cut(s) 177, 253, 493
PpuMI RGGWCCY 2 cut(s) 301, 760
Psp5II RGGWCCY 2 cut(s) 301, 760
Psp6I CCWGG 1 cut(s) 1168
PspGI CCWGG 1 cut(s) 1168
PspN4I GGNNCC 7 cut(s) 156, 165, 303, 351, 372, 679, 876
PspPI GGNCC 5 cut(s) 163, 301, 678, 700, 760
PspPPI RGGWCCY 2 cut(s) 301, 760
PstI CTGCAG 1 cut(s) 745
PstNI CAGNNNCTG 1 cut(s) 1094
PsuI RGATCY 4 cut(s) 154, 370, 768, 874
PsyI GACNNNGTC 1 cut(s) 143
RsaI GTAC 2 cut(s) 129, 1033
RsaNI GTAC 2 cut(s) 128, 1032
SacII CCGCGG 1 cut(s) 510
SaqAI TTAA 1 cut(s) 660
SatI GCNGC 4 cut(s) 176, 179, 954, 1006
Sau3AI GATC 9 cut(s) 154, 235, 295, 370, 421, 457, 768, 874, 945
Sau96I GGNCC 5 cut(s) 163, 301, 678, 700, 760
SchI GAGTC 3 cut(s) 178, 253, 493
ScrFI CCNGG 1 cut(s) 1170
SfaNI GCATC 3 cut(s) 52, 1186, 1197
SfcI CTRYAG 2 cut(s) 741, 933
Sfr274I CTCGAG 1 cut(s) 926
Sfr303I CCGCGG 1 cut(s) 510
SgrBI CCGCGG 1 cut(s) 510
SinI GGWCC 5 cut(s) 163, 301, 678, 700, 760
SlaI CTCGAG 1 cut(s) 926
SmlI CTYRAG 1 cut(s) 926
SmoI CTYRAG 1 cut(s) 926
Sse9I AATT 2 cut(s) 581, 981
SsiI CCGC 8 cut(s) 23, 173, 254, 263, 507, 509, 1157, 1218
SspMI CTAG 1 cut(s) 764
StyD4I CCNGG 1 cut(s) 1168
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 3 cut(s) 190, 934, 976
TaiI ACGT 1 cut(s) 998
TaqI TCGA 6 cut(s) 51, 502, 669, 927, 1062, 1211
TasI AATT 2 cut(s) 581, 981
TfiI GAWTC 2 cut(s) 9, 1079
Tru1I TTAA 1 cut(s) 660
Tru9I TTAA 1 cut(s) 660
TscAI CASTG 2 cut(s) 406, 442
TseFI GTSAC 2 cut(s) 496, 574
TseI GCWGC 4 cut(s) 175, 178, 953, 1005
Tsp45I GTSAC 2 cut(s) 496, 574
TspDTI ATGAA 5 cut(s) 22, 461, 528, 898, 909
TspGWI ACGGA 1 cut(s) 395
TspRI CASTG 2 cut(s) 406, 442
Tth111I GACNNNGTC 1 cut(s) 143
VpaK11BI GGWCC 5 cut(s) 163, 301, 678, 700, 760
XhoI CTCGAG 1 cut(s) 926
XspI CTAG 1 cut(s) 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.